Gene Engineering Division > Gene Set Collection > G1-S transition

G1-S transition

G1-S trasition


Summary of Cyclin function that regulates G1-S trasition
Cyclin Ds are upregulated in response to Ras-Map kinase signalling and assembled with cyclin D-dependent kinases (CDKs). The CDKs initiate Rb phosphorylation, releasing E2F from negative constraints and facilitating activation of a series of E2F-responsive genes, the products of which are necessary for S-phase entry. Activation of cyclin E by E2F enables formation of the cyclin E-cdk2 complex. This is accelerated by the continued sequestration of Cip/Kip proteins into complexes with assembling cyclin D-cdk complexes. Cyclin E-cdk2 completes the phosphorylation of Rb, further enabling activation of E2F-responsive genes, including cyclin A. Cyclin E-Cdk2 also stimulates DNA synthesis presumably by facilitating origin firing through Cdc45-dependent loading of DNA polymerase and promoting histone biosynthesis through direct phosphorylation of NPAT, a factor required for histone transcription. (Contributed by Dr. Koji Nakade)

Reporters

List of gene resources

Locus RDB no. Type Name of clone Running title
RB1 1278 DNA clone pTZRb1.05 Human Rb cDNA (amino acid 282-633)
PPP3CA 4132 ShuttleVector pAxCALNLhCalAalpha Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
PPP3CA 3559 ShuttleVector pAxCALNLhCalAalpha(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
MYC 6671 DNA clone pCMFlag_hsc-Myc Expression vector of human c-Myc
MYC 6435 DNA clone pENTR/hc-Myc
MYC 6432 DNA clone pGEM3Zf_hsc-Myc Plasmid clone of human c-Myc cDNA
MYC 6428 DNA clone pGEM3Zf_hsc-Myc(alternative translation initiations from an upstream) Plasmid clone of human MYC (alternative translation initiations from an upstream) cDNA
MYC 4659 ShuttleVector pAxCALNLhc-Myc(forward) Shuttle vector to generate rAd expressing human MYC
MYC 4650 ShuttleVector pAxCALNLhc-Myc(reverse) Shuttle vector to generate rAd expressing human MYC
Myc 2958 DNA clone mouse c-myc Plasmid clone of mouse c-myc cDNA, PCR fragment
E2F1 5537 DNA clone pKM2L-phE2F1(2) Promoter Bank clone, Human transcription factor E2F1 (E2F1) promoter
E2F1 5483 DNA clone pKM2L-phE2F1(1) Promoter Bank clone, Human transcription factor E2F1 (E2F1) promoter
Cdk4 6971 H3147D06 Cdk4, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25A 5476 DNA clone pKM2L-phCDC25A Promoter Bank clone, Human cdc25A promoter
CDC25A 4653 ShuttleVector pAxCALNLhCDC25A(forward) Shuttle vector to generate rAd expressing human CDC25A
CDC25A 4646 ShuttleVector pAxCALNLhCDC25A(reverse) Shuttle vector to generate rAd expressing human CDC25A
CCNH 6416 DNA clone pCMFlag_hsCCNH Expression vector of human CCNH
CCNH 4093 ShuttleVector pAxCALNLhCyclin H Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH 3510 ShuttleVector pAxCALNLhCyclin H(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND3 4589 DNA clone SEREX clone NGO-Br-20 (ID 859) #1 SEREX clone NGO-Br-20 (ID 859) #1
Camk2g 6890 DNA clone H4064B06 Camk2g, NIA/NIH Mouse 7.4K cDNA Clone (full CDS)
CAMK2B 4139 ShuttleVector pAxCALNLhCAMK2B Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B 4136 ShuttleVector pAxCALNLhCAMK2B Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B 4131 ShuttleVector pAxCALNLhCAMK2B Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B 3568 ShuttleVector pAxCALNLhCAMK2B(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B 3563 ShuttleVector pAxCALNLhCAMK2B(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B 3558 ShuttleVector pAxCALNLhCAMK2B(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK1G 4142 ShuttleVector pAxCALNLhCAMK1G Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK1G 3571 ShuttleVector pAxCALNLhCAMK1G(forward) Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
Camk1g 1782 DNA clone CaM kinase I gamma Rat calmodulin kinase I gamma cDNA

(GSB0008e, T.M. 2004.04.07)

2018.09.24



 

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