G1-S transition

G1-S trasition

Summary of Cyclin function that regulates G1-S trasitionCyclin Ds are upregulated in response to Ras-Map kinase signalling and assembled with cyclin D-dependent kinases (CDKs). The CDKs initiate Rb phosphorylation, releasing E2F from negative constraints and facilitating activation of a series of E2F-responsive genes, the products of which are necessary for S-phase entry. Activation of cyclin E by E2F enables formation of the cyclin E-cdk2 complex. This is accelerated by the continued sequestration of Cip/Kip proteins into complexes with assembling cyclin D-cdk complexes. Cyclin E-cdk2 completes the phosphorylation of Rb, further enabling activation of E2F-responsive genes, including cyclin A. Cyclin E-Cdk2 also stimulates DNA synthesis presumably by facilitating origin firing through Cdc45-dependent loading of DNA polymerase and promoting histone biosynthesis through direct phosphorylation of NPAT, a factor required for histone transcription. (Contributed by Dr. Koji Nakade)

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Reporters

• Fucci cell cycle indicators

List of gene resources

Locus	RDB no.	Type	Name of clone	Running title
RB1	1278	DNA clone	pTZRb1.05	Human Rb cDNA (amino acid 282-633)
PPP3CA	4132	ShuttleVector	pAxCALNLhCalAalpha	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
PPP3CA	3559	ShuttleVector	pAxCALNLhCalAalpha(forward)	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
MYC	6671	DNA clone	pCMFlag_hsc-Myc	Expression vector of human c-Myc
MYC	6435	DNA clone	pENTR/hc-Myc
MYC	6432	DNA clone	pGEM3Zf_hsc-Myc	Plasmid clone of human c-Myc cDNA
MYC	6428	DNA clone	pGEM3Zf_hsc-Myc(alternative translation initiations from an upstream)	Plasmid clone of human MYC (alternative translation initiations from an upstream) cDNA
MYC	4659	ShuttleVector	pAxCALNLhc-Myc(forward)	Shuttle vector to generate rAd expressing human MYC
MYC	4650	ShuttleVector	pAxCALNLhc-Myc(reverse)	Shuttle vector to generate rAd expressing human MYC
Myc	2958	DNA clone	mouse c-myc	Plasmid clone of mouse c-myc cDNA, PCR fragment
E2F1	5537	DNA clone	pKM2L-phE2F1(2)	Promoter Bank clone, Human transcription factor E2F1 (E2F1) promoter
E2F1	5483	DNA clone	pKM2L-phE2F1(1)	Promoter Bank clone, Human transcription factor E2F1 (E2F1) promoter
Cdk4	6971		H3147D06	Cdk4, NIA/NIH Mouse 15K cDNA Clone (full CDS)
CDC25A	5476	DNA clone	pKM2L-phCDC25A	Promoter Bank clone, Human cdc25A promoter
CDC25A	4653	ShuttleVector	pAxCALNLhCDC25A(forward)	Shuttle vector to generate rAd expressing human CDC25A
CDC25A	4646	ShuttleVector	pAxCALNLhCDC25A(reverse)	Shuttle vector to generate rAd expressing human CDC25A
CCNH	6416	DNA clone	pCMFlag_hsCCNH	Expression vector of human CCNH
CCNH	4093	ShuttleVector	pAxCALNLhCyclin H	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCNH	3510	ShuttleVector	pAxCALNLhCyclin H(forward)	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CCND3	4589	DNA clone	SEREX clone NGO-Br-20 (ID 859) #1	SEREX clone NGO-Br-20 (ID 859) #1
Camk2g	6890	DNA clone	H4064B06	Camk2g, NIA/NIH Mouse 7.4K cDNA Clone (full CDS)
CAMK2B	4139	ShuttleVector	pAxCALNLhCAMK2B	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B	4136	ShuttleVector	pAxCALNLhCAMK2B	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B	4131	ShuttleVector	pAxCALNLhCAMK2B	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B	3568	ShuttleVector	pAxCALNLhCAMK2B(forward)	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B	3563	ShuttleVector	pAxCALNLhCAMK2B(forward)	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK2B	3558	ShuttleVector	pAxCALNLhCAMK2B(forward)	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK1G	4142	ShuttleVector	pAxCALNLhCAMK1G	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
CAMK1G	3571	ShuttleVector	pAxCALNLhCAMK1G(forward)	Shuttle vector to generate rAd harboring Sugano full-length cDNA clone
Camk1g	1782	DNA clone	CaM kinase I gamma	Rat calmodulin kinase I gamma cDNA

(GSB0008e, T.M. 2004.04.07)

2018.09.24