Expression Vector Backbone of S. pombe | Gene Engineering Division - DNA BANK

Empty expression vector backbone of Schizosaccharomyces pombe

Reference: PLoS One 9, e112488 (2014)., PMID 25401760.

Catalog No.	plasmid	backborne	Sp marker	Ec marker	insert	remarks
RDB08041	pCtrlki-kanMX6	bacterial	kanMX6	Ap	MCS1-opal stop codon-kanMX6-MCS2	Plasmid vector to constract C-terminal truncated allele by the integration of opal stop codon into target gene using homologous recombination.
RDB08042	pCtFEM3ki-kanMX6	bacterial	kanMX6	Ap	MCS1-Flag-tev-3myc-opal stop codon-kanMX6-MCS2	Plasmid vector to construct C-terminally FEM3 (FLAG-tev-3xmyc) tagged allele of target gene by using homologous recombination.
RDB08045	pCtFLATAki-kanMX6	bacterial	kanMX6	Ap	MCS1-Flag-tev-protein A-opal stop codon-kanMX6-MCS2	Plasmid vector to construct C-terminally FLATA (FLAG-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08048	pFOX1-CFLATA	replicative	ScLEU2 (complement leu1-)	Ap	P_tef-MCS1-Flag-tev-protein A-opal stop codon-T_tef	Plasmid vector to constract replicative expression clone constitutively expressing C-terminally FLATA tag (FLAG-tev-protein A) allele in leu1- strain of S. pombe.
RDB08051	pCtHATAki-kanMX6	bacterial	kanMX6	Ap	MCS1-HA-tev-protein A-opal stop codon-kanMX6-MCS2	Plasmid vector to constract C-terminally HATA (HA-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08052	pFOX1-CHATA	replicative	ScLEU2 (complement leu1-)	Ap	P_tef-MCS1-HA-tev-protein A-opal stop codon-T_tef	Plasmid vector to constract replicative expression clone constitutively expressing C-terminally HATA tag (HA-tev-protein A) allele in leu1- strain of S. pombe.

Depositor:
Dr. Akihisa Matsuyama,
Dr. Minoru Yoshida

S. pombe expression vectors (Gateway compatible)

Reference

Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.
Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M. ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotechnol. 2006 Jul; 24(7):841-7.
Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.
Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL series (Gateway-compatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06130	pDUAL-HFF1c	pDUAL	His₆-FLAG₂	5′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06485	pDUAL-HFF21c	pDUAL	His₆-FLAG₂	5′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06486	pDUAL-HFF31c	pDUAL	His₆-FLAG₂	5′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06131	pDUAL-HFF41c	pDUAL	His₆-FLAG₂	5′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06487	pDUAL-HFF51c	pDUAL	His₆-FLAG₂	5′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06488	pDUAL-HFF61c	pDUAL	His₆-FLAG₂	5′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06132	pDUAL-HFF81c	pDUAL	His₆-FLAG₂	5′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06133	pDUAL-HFG1c	pDUAL	His₆-FLAG-GFP(S65C)	5′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06493	pDUAL-HFG21c	pDUAL	His₆-FLAG-GFP(S65C)	5′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06494	pDUAL-HFG31c	pDUAL	His₆-FLAG-GFP(S65C)	5′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06134	pDUAL-HFG41c	pDUAL	His₆-FLAG-GFP(S65C)	5′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06495	pDUAL-HFG51c	pDUAL	His₆-FLAG-GFP(S65C)	5′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06496	pDUAL-HFG61c	pDUAL	His₆-FLAG-GFP(S65C)	5′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06135	pDUAL-HFG81c	pDUAL	His₆-FLAG-GFP(S65C)	5′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06136	pDUAL-HFC1c	pDUAL	His₆-FLAG-CFP	5′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06137	pDUAL-HFC41c	pDUAL	His₆-FLAG-CFP	5′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06138	pDUAL-HFC81c	pDUAL	His₆-FLAG-CFP	5′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06139	pDUAL-HFY1c	pDUAL	His₆-FLAG-YFP	5′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06140	pDUAL-HFY41c	pDUAL	His₆-FLAG-YFP	5′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06141	pDUAL-HFY81c	pDUAL	His₆-FLAG-YFP	5′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06142	pDUAL-FFH1c	pDUAL	FLAG₂-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06489	pDUAL-FFH21c	pDUAL	FLAG₂-His₆	3′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06490	pDUAL-FFH31c	pDUAL	FLAG₂-His₆	3′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06143	pDUAL-FFH41c	pDUAL	FLAG₂-His₆	3′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06491	pDUAL-FFH51c	pDUAL	FLAG₂-His₆	3′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06492	pDUAL-FFH61c	pDUAL	FLAG₂-His₆	3′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06144	pDUAL-FFH81c	pDUAL	FLAG₂-His₆	3′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06145	pDUAL-GFH1c	pDUAL	GFP(S65C)-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06497	pDUAL-GFH21c	pDUAL	GFP(S65C)-FLAG-His₆	3′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06498	pDUAL-GFH31c	pDUAL	GFP(S65C)-FLAG-His₆	3′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06146	pDUAL-GFH41c	pDUAL	GFP(S65C)-FLAG-His₆	3′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06499	pDUAL-GFH51c	pDUAL	GFP(S65C)-FLAG-His₆	3′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06500	pDUAL-GFH61c	pDUAL	GFP(S65C)-FLAG-His₆	3′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06147	pDUAL-GFH81c	pDUAL	GFP(S65C)-FLAG-His₆	3′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06148	pDUAL-CFH1c	pDUAL	CFP-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06149	pDUAL-CFH41c	pDUAL	CFP-FLAG-His₆	3′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06150	pDUAL-CFH81c	pDUAL	CFP-FLAG-His₆	3′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06151	pDUAL-YFH1c	pDUAL	YFP-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06152	pDUAL-YFH41c	pDUAL	YFP-FLAG-His₆	3′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06153	pDUAL-YFH81c	pDUAL	YFP-FLAG-His₆	3′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06481	pDUAL-FFH1c rfA	pDUAL	FLAG₂-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06482	pDUAL-FFH1c rfC	pDUAL	FLAG₂-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06483	pDUAL-YFH1c rfA	pDUAL	YFP-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06484	pDUAL-YFH1c rfC	pDUAL	YFP-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL2 series (Gateway-compatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06154	pDUAL2-HFF1c	pDUAL2	His₆-FLAG₂	5′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06155	pDUAL2-HFF41c	pDUAL2	His₆-FLAG₂	5′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06156	pDUAL2-HFF81c	pDUAL2	His₆-FLAG₂	5′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06157	pDUAL2-HFG1c	pDUAL2	His₆-FLAG-GFP(S65C)	5′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06158	pDUAL2-HFG41c	pDUAL2	His₆-FLAG-GFP(S65C)	5′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06159	pDUAL2-HFG81c	pDUAL2	His₆-FLAG-GFP(S65C)	5′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06160	pDUAL2-HFC1c	pDUAL2	His₆-FLAG-CFP	5′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06161	pDUAL2-HFC41c	pDUAL2	His₆-FLAG-CFP	5′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06162	pDUAL2-HFC81c	pDUAL2	His₆-FLAG-CFP	5′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06163	pDUAL2-HFY1c	pDUAL2	His₆-FLAG-YFP	5′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06164	pDUAL2-HFY41c	pDUAL2	His₆-FLAG-YFP	5′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06165	pDUAL2-HFY81c	pDUAL2	His₆-FLAG-YFP	5′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06166	pDUAL2-FFH1c	pDUAL2	FLAG₂-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06167	pDUAL2-FFH41c	pDUAL2	FLAG₂-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06168	pDUAL2-FFH81c	pDUAL2	FLAG₂-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06169	pDUAL2-GFH1c	pDUAL2	GFP(S65C)-FLAG-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06170	pDUAL2-GFH41c	pDUAL2	GFP(S65C)-FLAG-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06171	pDUAL2-GFH81c	pDUAL2	GFP(S65C)-FLAG-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06172	pDUAL2-CFH1c	pDUAL2	CFP-FLAG-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06173	pDUAL2-CFH41c	pDUAL2	CFP-FLAG-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06174	pDUAL2-CFH81c	pDUAL2	CFP-FLAG-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06175	pDUAL2-YFH1c	pDUAL2	YFP-FLAG-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06503	pDUAL2-YFH21c	pDUAL2	YFP-FLAG-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06176	pDUAL2-YFH41c	pDUAL2	YFP-FLAG-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06177	pDUAL2-YFH81c	pDUAL2	YFP-FLAG-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06501	pDUAL2-BFH41c	pDUAL2	BFP-FLAG-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06502	pDUAL2-BFH81c	pDUAL2	BFP-FLAG-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pREP series (Gateway-compatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Selection marker
RDB06519	pREP1-ccdB2	pREP1	–	–	nmt1	LEU2	[sequence]
RDB06520	pREP41-ccdB2	pREP41	–	–	nmt1*	LEU2	[sequence]
RDB06521	pREP81-ccdB2	pREP81	–	–	nmt1**	LEU2	[sequence]
RDB06522	pHFF1-ccdB2	pREP1	His₆-FLAG₂	5′	nmt1	LEU2	[sequence]
RDB06523	pHFF41-ccdB2	pREP41	His₆-FLAG₂	5′	nmt1*	LEU2	[sequence]
RDB06524	pHFF81-ccdB2	pREP81	His₆-FLAG₂	5′	nmt1**	LEU2	[sequence]
RDB06525	pHFG1-ccdB2	pREP1	His₆-FLAG-GFP(S65C)	5′	nmt1	LEU2	[sequence]
RDB06526	pHFG41-ccdB2	pREP41	His₆-FLAG-GFP(S65C)	5′	nmt1*	LEU2	[sequence]
RDB06527	pHFG81-ccdB2	pREP81	His₆-FLAG-GFP(S65C)	5′	nmt1**	LEU2	[sequence]
RDB06506	pFFH1-ccdB2	pREP1	FLAG₂-His₆	3′	nmt1	LEU2	[sequence]
RDB06507	pFFH41-ccdB2	pREP41	FLAG₂-His₆	3′	nmt1*	LEU2	[sequence]
RDB06508	pFFH81-ccdB2	pREP81	FLAG₂-His₆	3′	nmt1**	LEU2	[sequence]
RDB06509	pGFH1-ccdB2	pREP1	GFP(S65C)-FLAG-His₆	3′	nmt1	LEU2	[sequence]
RDB06510	pGFH41-ccdB2	pREP41	GFP(S65C)-FLAG-His₆	3′	nmt1*	LEU2	[sequence]
RDB06511	pGFH81-ccdB2	pREP81	GFP(S65C)-FLAG-His₆	3′	nmt1**	LEU2	[sequence]
RDB06513	pHFY1-ccdB2	pREP1	His₆-FLAG-YFP	5′	nmt1	LEU2	[sequence]
RDB06512	pHFC1-ccdB2	pREP1	His₆-FLAG-CFP	5′	nmt1	LEU2	[sequence]
RDB06515	pYFH1-ccdB2	pREP1	YFP-FLAG-His₆	3′	nmt1	LEU2	[sequence]
RDB06505	pYFH1-ccdB2 rfA	pREP1	YFP-FLAG-His₆	3′	nmt1	LEU2	[sequence]
RDB06504	pYFH1-ccdB2 rfC	pREP1	YFP-FLAG-His₆	3′	nmt1	LEU2	[sequence]
RDB06514	pCFH1-ccdB2	pREP1	CFP-FLAG-His₆	3′	nmt1	LEU2	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

Gateway-compatible vectors for chromosomal integration

Cat. num.	Plasmid name	Backbone	Promoter	Tag	Tag position	Multicopy marker	Targeted locus (negative) selection	Positive selection marker
RDB06462	pLYS1U-CFH1c	pUC119	nmt1	CFP-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06463	pLYS1U-CFH21c	pUC119	tub1	CFP-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06464	pLYS1U-CFH61c	pUC119	ef1a-c	CFP-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06465	pLYS1U-FFH1c	pUC119	nmt1	FLAG₂-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06466	pLYS1U-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06467	pLYS1U-GFH21c	pUC119	tub1	GFP(S65C)-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06468	pLYS1U-GFH61c	pUC119	ef1a-c	GFP(S65C)-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06477	pLYS1U-YFH1c	pUC119	nmt1	YFP-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06478	pLYS1U-YFH21c	pUC119	tub1	YFP-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06479	pLYS1U-YFH61c	pUC119	ef1a-c	YFP-FLAG-His₆	3′	–	lys1⁺	ura4⁺	[sequence]
RDB06469	pLYS1U-HFC1c	pUC119	nmt1	His₆-FLAG-CFP	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06470	pLYS1U-HFC21c	pUC119	tub1	His₆-FLAG-CFP	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06471	pLYS1U-HFC61c	pUC119	ef1a-c	His₆-FLAG-CFP	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06472	pLYS1U-HFF1c	pUC119	nmt1	His₆-FLAG₂	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06473	pLYS1U-HFG1c	pUC119	nmt1	His₆-FLAG-GFP(S65C)	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06474	pLYS1U-HFY1c	pUC119	nmt1	His₆-FLAG-YFP	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06475	pLYS1U-HFY21c	pUC119	tub1	His₆-FLAG-YFP	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06476	pLYS1U-HFY61c	pUC119	ef1a-c	His₆-FLAG-YFP	5′	–	lys1⁺	ura4⁺	[sequence]
RDB06458	pLYS1K-FFH1c	pUC119	nmt1	FLAG₂-His₆	3′	–	lys1⁺	kan^r	[sequence]
RDB06459	pLYS1K-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His₆	3′	–	lys1⁺	kan^r	[sequence]
RDB06460	pLYS1K-HFF1c	pUC119	nmt1	His₆-FLAG₂	5′	–	lys1⁺	kan^r	[sequence]
RDB06461	pLYS1K-HFG1c	pUC119	nmt1	His₆-FLAG-GFP(S65C)	5′	–	lys1⁺	kan^r	[sequence]
RDB06517	pHIS3U-CFH1c	pUC119	nmt1	CFP-FLAG-His₆	3′	–	his3⁺	ura4⁺	[sequence]
RDB06455	pHIS3U-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His₆	3′	–	his3⁺	ura4⁺	[sequence]
RDB06518	pHIS3U-YFH1c	pUC119	nmt1	YFP-FLAG-His₆	3′	–	his3⁺	ura4⁺	[sequence]
RDB06456	pHIS3U-HFF1c	pUC119	nmt1	His₆-FLAG₂	5′	–	his3⁺	ura4⁺	[sequence]
RDB06516	pHIS3U-HFC1c	pUC119	nmt1	His₆-FLAG-CFP	5′	–	his3⁺	ura4⁺	[sequence]
RDB06457	pHIS3U-HFG1c	pUC119	nmt1	His₆-FLAG-GFP(S65C)	5′	–	his3⁺	ura4⁺	[sequence]
RDB06445	pHIS3K-CFH1c	pUC119	nmt1	CFP-FLAG-His₆	3′	–	his3⁺	kan^r	[sequence]
RDB06446	pHIS3K-CFH21c	pUC119	tub1	CFP-FLAG-His₆	3′	–	his3⁺	kan^r	[sequence]
RDB06447	pHIS3K-CFH61c	pUC119	ef1a-c	CFP-FLAG-His₆	3′	–	his3⁺	kan^r	[sequence]
RDB06448	pHIS3K-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His₆	3′	–	his3⁺	kan^r	[sequence]
RDB06449	pHIS3K-GFH21c	pUC119	tub1	GFP(S65C)-FLAG-His₆	3′	–	his3⁺	kan^r	[sequence]
RDB06450	pHIS3K-GFH61c	pUC119	ef1a-c	GFP(S65C)-FLAG-His₆	3′	–	his3⁺	kan^r	[sequence]
RDB06451	pHIS3K-HFC1c	pUC119	nmt1	His₆-FLAG-CFP	5′	–	his3⁺	kan^r	[sequence]
RDB06452	pHIS3K-HFC21c	pUC119	tub1	His₆-FLAG-CFP	5′	–	his3⁺	kan^r	[sequence]
RDB06453	pHIS3K-HFF1c	pUC119	nmt1	His₆-FLAG₂	5′	–	his3⁺	kan^r	[sequence]
RDB06454	pHIS3K-HFG1c	pUC119	nmt1	His₆-FLAG-GFP(S65C)	5′	–	his3⁺	kan^r	[sequence]
RDB06441	pARG1U-FFH1c	pUC119	nmt1	FLAG₂-His₆	3′	–	arg1⁺	ura4⁺	[sequence]
RDB06442	pARG1U-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His₆	3′	–	arg1⁺	ura4⁺	[sequence]
RDB06443	pARG1U-HFF1c	pUC119	nmt1	His₆-FLAG₂	5′	–	arg1⁺	ura4⁺	[sequence]
RDB06444	pARG1U-HFG1c	pUC119	nmt1	His₆-FLAG-GFP(S65C)	5′	–	arg1⁺	ura4⁺	[sequence]
RDB06437	pARG1K-FFH1c	pUC119	nmt1	FLAG₂-His₆	3′	–	arg1⁺	kan^r	[sequence]
RDB06438	pARG1K-GFH1c	pUC119	nmt1	GFP(S65C)-FLAG-His₆	3′	–	arg1⁺	kan^r	[sequence]
RDB06439	pARG1K-HFF1c	pUC119	nmt1	His₆-FLAG₂	5′	–	arg1⁺	kan^r	[sequence]
RDB06440	pARG1K-HFG1c	pUC119	nmt1	His₆-FLAG-GFP(S65C)	5′	–	arg1⁺	kan^r	[sequence]
RDB06480	pARG1KF-CFH1c	pUC119	nmt1	CFP-FLAG-His₆	3′	ura4⁺	arg1⁺	kan^r	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

S. pombe expression vectors

Reference

Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.
Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.
Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL series (Gateway-incompatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06178	pDUAL	(pUC119)	–	–	–	ura4⁺	leu1⁺	[sequence]
RDB06528	pDUAL-S	(pUC119)	–	–	–	ura4⁺	leu1⁺	[sequence]
RDB06529	pDUAL-Pef1a	pDUAL	–	–	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06530	pDUAL-Ptub1	pDUAL	–	–	tub1	ura4⁺	leu1⁺	[sequence]
RDB06179	pDUAL-HFF1	pDUAL	His₆-FLAG₂	5′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06531	pDUAL-HFF21	pDUAL	His₆-FLAG₂	5′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06532	pDUAL-HFF31	pDUAL	His₆-FLAG₂	5′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06180	pDUAL-HFF41	pDUAL	His₆-FLAG₂	5′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06533	pDUAL-HFF51	pDUAL	His₆-FLAG₂	5′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06534	pDUAL-HFF61	pDUAL	His₆-FLAG₂	5′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06181	pDUAL-HFF81	pDUAL	His₆-FLAG₂	5′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06182	pDUAL-HFG1	pDUAL	His₆-FLAG-GFP(S65C)	5′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06539	pDUAL-HFG21	pDUAL	His₆-FLAG-GFP(S65C)	5′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06540	pDUAL-HFG31	pDUAL	His₆-FLAG-GFP(S65C)	5′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06183	pDUAL-HFG41	pDUAL	His₆-FLAG-GFP(S65C)	5′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06541	pDUAL-HFG51	pDUAL	His₆-FLAG-GFP(S65C)	5′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06542	pDUAL-HFG61	pDUAL	His₆-FLAG-GFP(S65C)	5′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06184	pDUAL-HFG81	pDUAL	His₆-FLAG-GFP(S65C)	5′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06185	pDUAL-FFH1	pDUAL	FLAG₂-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06535	pDUAL-FFH21	pDUAL	FLAG₂-His₆	3′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06536	pDUAL-FFH31	pDUAL	FLAG₂-His₆	3′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06186	pDUAL-FFH41	pDUAL	FLAG₂-His₆	3′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06537	pDUAL-FFH51	pDUAL	FLAG₂-His₆	3′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06538	pDUAL-FFH61	pDUAL	FLAG₂-His₆	3′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06187	pDUAL-FFH81	pDUAL	FLAG₂-His₆	3′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06188	pDUAL-GFH1	pDUAL	GFP(S65C)-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]
RDB06543	pDUAL-GFH21	pDUAL	GFP(S65C)-FLAG-His₆	3′	tub1	ura4⁺	leu1⁺	[sequence]
RDB06544	pDUAL-GFH31	pDUAL	GFP(S65C)-FLAG-His₆	3′	cam1	ura4⁺	leu1⁺	[sequence]
RDB06189	pDUAL-GFH41	pDUAL	GFP(S65C)-FLAG-His₆	3′	nmt1*	ura4⁺	leu1⁺	[sequence]
RDB06545	pDUAL-GFH51	pDUAL	GFP(S65C)-FLAG-His₆	3′	tif51	ura4⁺	leu1⁺	[sequence]
RDB06546	pDUAL-GFH61	pDUAL	GFP(S65C)-FLAG-His₆	3′	ef1a-c	ura4⁺	leu1⁺	[sequence]
RDB06190	pDUAL-GFH81	pDUAL	GFP(S65C)-FLAG-His₆	3′	nmt1**	ura4⁺	leu1⁺	[sequence]
RDB06191	pDUAL-YFH1	pDUAL	YFP-FLAG-His₆	3′	nmt1	ura4⁺	leu1⁺	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL2 series (Gateway-incompatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Multicopy marker	Integration marker
RDB06192	pDUAL2	(pUC119)	–	–	–	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06193	pDUAL2-HFF1	pDUAL2	His₆-FLAG₂	5′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06547	pDUAL2-HFF21	pDUAL2	His₆-FLAG₂	5′	tub1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06194	pDUAL2-HFF41	pDUAL2	His₆-FLAG₂	5′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06549	pDUAL2-HFF61	pDUAL2	His₆-FLAG₂	5′	ef1a-c	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06195	pDUAL2-HFF81	pDUAL2	His₆-FLAG₂	5′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06196	pDUAL2-HFG1	pDUAL2	His₆-FLAG-GFP(S65C)	5′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06197	pDUAL2-HFG41	pDUAL2	His₆-FLAG-GFP(S65C)	5′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06198	pDUAL2-HFG81	pDUAL2	His₆-FLAG-GFP(S65C)	5′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06199	pDUAL2-FFH1	pDUAL2	FLAG₂-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06548	pDUAL2-FFH21	pDUAL2	FLAG₂-His₆	3′	tub1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06200	pDUAL2-FFH41	pDUAL2	FLAG₂-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06550	pDUAL2-FFH61	pDUAL2	FLAG₂-His₆	3′	ef1a-c	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06201	pDUAL2-FFH81	pDUAL2	FLAG₂-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06202	pDUAL2-GFH1	pDUAL2	GFP(S65C)-FLAG-His₆	3′	nmt1	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06203	pDUAL2-GFH41	pDUAL2	GFP(S65C)-FLAG-His₆	3′	nmt1*	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06204	pDUAL2-GFH81	pDUAL2	GFP(S65C)-FLAG-His₆	3′	nmt1**	ura4⁺	ura4⁺, leu1^–	[sequence]
RDB06551	pDUAL2-CFH61	pDUAL2	CFP-FLAG-His₆	3′	ef1a-c	ura4⁺	ura4⁺, leu1^–	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pREP series (Gateway-incompatible)

Cat. num.	Plasmid name	Backbone	Tag	Tag position	Promoter	Selection marker
RDB06580	pHFF1	pREP1	His₆-FLAG₂	5′	nmt1	LEU2	[sequence]
RDB06581	pHFF41	pREP41	His₆-FLAG₂	5′	nmt1*	LEU2	[sequence]
RDB06582	pHFF81	pREP81	His₆-FLAG₂	5′	nmt1**	LEU2	[sequence]
RDB06592	pHFF148	(pREP)	His₆-FLAG₂	5′	ADH1	LEU2	[sequence]
RDB06583	pHFG1	pREP1	His₆-FLAG-GFP(S65C)	5′	nmt1	LEU2	[sequence]
RDB06584	pHFG41	pREP41	His₆-FLAG-GFP(S65C)	5′	nmt1*	LEU2	[sequence]
RDB06585	pHFG81	pREP81	His₆-FLAG-GFP(S65C)	5′	nmt1**	LEU2	[sequence]
RDB06593	pHFG148	(pREP)	His₆-FLAG-GFP(S65C)	5′	ADH1	LEU2	[sequence]
RDB06586	pFFH1	pREP1	FLAG₂-His₆	3′	nmt1	LEU2	[sequence]
RDB06587	pFFH41	pREP41	FLAG₂-His₆	3′	nmt1*	LEU2	[sequence]
RDB06588	pFFH81	pREP81	FLAG₂-His₆	3′	nmt1**	LEU2	[sequence]
RDB06594	pFFH148	(pREP)	FLAG₂-His₆	3′	ADH1	LEU2	[sequence]
RDB06589	pGFH1	pREP1	GFP(S65C)-FLAG-His₆	3′	nmt1	LEU2	[sequence]
RDB06590	pGFH41	pREP41	GFP(S65C)-FLAG-His₆	3′	nmt1*	LEU2	[sequence]
RDB06591	pGFH81	pREP81	GFP(S65C)-FLAG-His₆	3′	nmt1**	LEU2	[sequence]
RDB06595	pGFH148	(pREP)	GFP(S65C)-FLAG-His₆	3′	ADH1	LEU2	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

Gateway-incompatible vectors for chromosomal integration

Cat. num.	Plasmid name	Backbone	Promoter	Multicopy marker	Targeted locus	Positive selection marker
RDB06552	pLYS1U	pUC119	–	–	lys1⁺	ura4⁺	[sequence]
RDB06567	pLYS1U-Pnmt1	pUC119	nmt1	–	lys1⁺	ura4⁺	[sequence]
RDB06566	pLYS1U-Ptub1	pUC119	tub1	–	lys1⁺	ura4⁺	[sequence]
RDB06553	pLYS1K	pUC119	–	–	lys1⁺	kan^r	[sequence]
RDB06554	pLYS1H	pUC119	–	–	lys1⁺	hyg^r	[sequence]
RDB06555	pLYS1B	pUC119	–	–	lys1⁺	bla^r	[sequence]
RDB06560	pHIS3U	pUC119	–	–	his3⁺	ura4⁺	[sequence]
RDB06565	pHIS3U-Pnmt1	pUC119	nmt1	–	his3⁺	ura4⁺	[sequence]
RDB06561	pHIS3K	pUC119	–	–	his3⁺	kan^r	[sequence]
RDB06564	pHIS3K-Ptub1	pUC119	tub1	–	his3⁺	kan^r	[sequence]
RDB06562	pHIS3H	pUC119	–	–	his3⁺	hyg^r	[sequence]
RDB06563	pHIS3B	pUC119	–	–	his3⁺	bla^r	[sequence]
RDB06556	pARG1U	pUC119	–	–	arg1⁺	ura4⁺	[sequence]
RDB06557	pARG1K	pUC119	–	–	arg1⁺	kan^r	[sequence]
RDB06558	pARG1H	pUC119	–	–	arg1⁺	hyg^r	[sequence]
RDB06559	pARG1B	pUC119	–	–	arg1⁺	bla^r	[sequence]
RDB06573	pLYS1UF	pUC119	–	ura4⁺	lys1⁺	ura4⁺	[sequence]
RDB06572	pLYS1KF	pUC119	–	ura4⁺	lys1⁺	kan^r	[sequence]
RDB06574	pLYS1HF	pUC119	–	ura4⁺	lys1⁺	hyg^r	[sequence]
RDB06575	pLYS1BF	pUC119	–	ura4⁺	lys1⁺	bla^r	[sequence]
RDB06569	pHIS3UF	pUC119	–	ura4⁺	his3⁺	ura4⁺	[sequence]
RDB06568	pHIS3KF	pUC119	–	ura4⁺	his3⁺	kan^r	[sequence]
RDB06570	pHIS3HF	pUC119	–	ura4⁺	his3⁺	hyg^r	[sequence]
RDB06571	pHIS3BF	pUC119	–	ura4⁺	his3⁺	bla^r	[sequence]
RDB06577	pARG1UF	pUC119	–	ura4⁺	arg1⁺	ura4⁺	[sequence]
RDB06576	pARG1KF	pUC119	–	ura4⁺	arg1⁺	kan^r	[sequence]
RDB06578	pARG1HF	pUC119	–	ura4⁺	arg1⁺	hyg^r	[sequence]
RDB06579	pARG1BF	pUC119	–	ura4⁺	arg1⁺	bla^r	[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

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(GRP0027e 2011.09.30 T.M.)

2023.06.08