Clone Search

Resources for

Almost all our operation is carried out normally and smoothly but it will take about a month to ship after the reception. We appreciate your understanding and patience in the cases that these inconveniences occur.

Clones & Vectors for Gene Expression
  in E. coli
  in T. thermophilus HB8
  in S. pombe
  in S. cerevisiae
  in Mammalian Cells

Research Tools
  Genome Editing
  Fluorescent Proteins
  Luminescent Proteins
  Sensor & Visualization
  Plant gene resources

Clone Set, Library & Genomic DNA
  Genomic clone
  cDNA clone
  Expression clone
  Libraries
  Genomic DNA

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vectors

Gene Set Collection
  Autophagy
  Circadian Clock
  Notch Signaling
  Sphingolipid Signaling

Search and Browse
  Key word search
  Browsing by category
  Browsing by species
  Depositors List

Takara Bio USA, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, such as DsRed2 and mCherry for academic use

BRC News

Expression Vector Backbone of Saccharomyces cerevisiae


CRITGI, a new tool to control gene induction in budding yeast

CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner.

Hanasaki M, Masumoto H.
Sci. Rep. 9 (1): 15300 (2019). PubMed PMID 31653950.

Catalog no. Name Running title
RDB18135 PHM661 Integration plasmid to Ty1 loci, YIplac128 back bone (pTy1). CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18136 PHM688 Integration plasmid to Ty1 loci, pRS403 back bone. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18137 PHM663 Cas9 and sgRNA plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18138 PHM664 Cas9 and sgRNA #4 plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18139 PHM740 Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18140 PHM760 Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18141 PHM745 Integration plasmid with TDH3 promoter to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18142 PHM757 Integration plasmid expressing histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18143 PHM829 Integration plasmid of histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18144 PHM820 Integration plasmid expressing Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18145 PHM833 Integration plasmid expressing indolepyruvate decarboxylase 1 (PDC1) to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18146 PHM821 Integration plasmid of histone H3 (HHT1) and Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.
RDB18147 PHM802 Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Saccharomyces cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli

Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals.

Iida, K., Teng, J., Tada, T., Saka, A., Tamai, M., Izumi-Nakaseko, H., Adachi-Akahane, S., Iida, H.
J. Biol. Chem. 282 (35): 25659-25667 (2007). PubMed PMID 17569661.

Catalog no. Name Running title
RDB15296 pBC111 S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.

Empty expression vector designed to produce GFP-tagged protein

Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1

Sasaki, T., Takagi, H.
Genes Cells, 18 (6): 459-475 (2013). PubMed PMID 23517290.

Catalog no. Name Running title
RDB08380 pRS416-PADH1-ccdB-yEGFP-TTEF1 Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria.
RDB08386 pRS416-PADH1-yEGFP-TTEF1 Empty expression vector backbone of S. cerevisiae

Expression vector of S. cerevisiae Singlecopy plasmid

Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants.

Matsuda, N., Ueda, T., Sasaki, Y., Nakano, A.
Cell. Struct. Funct. 25 (1): 11-20 (2000). PubMed PMID 10791890.

Catalog no. Name Running title
RDB08655 pSKY5 Expression vector of S. cerevisiae. Single copy plasmid harboring the TDH3 promoter, EGFP, CMK1 terminator and the URA3 marker.
RDB08752 pYO773 Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the URA3 marker.
RDB08753 pNMV3 Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the HIS3 marker.
RDB08754 pNMV4 Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the TRP1 marker.

Expression vector designed to produce C-terminal-Myc-tagged fusion proteins

Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins

Iha, H., Tsurugi, K.
Biotechniques, 25, 936-938 (1998). PubMed PMID 9863043.

Catalog no. Name Tag Ori Marker Promoter Size (kb) Usable sites
RDB01947 pAMH10 Myc centromere-type HIS3 ADH1 4.5 6
RDB01948 pAMH20 Myc 2micron HIS3 ADH1 5.3 6
RDB01949 pAML10 Myc centromere-type LEU2 ADH1 5.1 6
RDB01950 pAML20 Myc 2micron LEU2 ADH1 5.9 6
RDB01951 pAMT10 Myc centromere-type TRP1 ADH1 4.3 5
RDB01952 pAMT20 Myc 2micron TRP1 ADH1 5.1 5
RDB01953 pAMU10 Myc centromere-type URA3 ADH1 4.6 6
RDB01954 pAMU20 Myc 2micron URA3 ADH1 5.4 6
RDB01955 pGMH10 Myc centromere-type HIS3 GAL1-10 4.8 6
RDB01956 pGMH20 Myc 2micron HIS3 GAL1-10 5.6 6
RDB01957 pGML10 Myc centromere-type LEU2 GAL1-10 5.4 6
RDB01958 pGML20 Myc 2micron LEU2 GAL1-10 6.2 6
RDB01959 pGMT10 Myc centromere-type TRP1 GAL1-10 4.6 5
RDB01960 pGMT20 Myc 2micron TRP1 GAL1-10 5.4 5
RDB01961 pGMU10 Myc centromere-type URA3 GAL1-10 4.9 6
RDB01962 pGMU20 Myc 2micron URA3 GAL1-10 5.7 6

Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.


National BioResource Project – Yeast
Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.

go to Head


(GRP0047e 2008.05.20 T.M.)

2020.10.15