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BRC News

Expression Vector Backbone of Saccharomyces cerevisiae


Saccharomyces cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli

Depositor: Dr. Hidetoshi Iida
Reference:
Cho, T. et al. Genes Cells 22 (1): 94-104 (2017). PMID:27935186.
Iida, K. et al. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

Catalog no. Name Running title
RDB15296 pBC111 S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.

Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein

Depositor: Dr. Hiroshi Takagi
Reference: Sasaki, T., Takagi, H. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.

Catalog no. Name Running title
RDB08380 pRS416-PADH1-ccdB-yEGFP-TTEF1 Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria.
RDB08386 pRS416-PADH1-yEGFP-TTEF1 Empty expression vector backbone of S. cerevisiae

Expression vector of S. cerevisiae Singlecopy plasmid

Depositor: Dr. Akihiko Nakano
Reference: Sato, K., Sato, M., Nakano, A. J. Cell Biol. 152 (5): 935-944. PMID 11238450.
Reference: Matsuda, N., Ueda, T., Sasaki, Y., Nakano, A. Cell. Struct. Funct. 25 (1): 11-20 (2000). PMID 10791890.

Catalog no. Name Running title
RDB08655 pSKY5 Expression vector of S. cerevisiae. Single copy plasmid harboring the TDH3 promoter, EGFP, CMK1 terminator and the URA3 marker.
RDB08752 pYO773 Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the URA3 marker.
RDB08753 pNMV3 Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the HIS3 marker.
RDB08754 pNMV4 Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the TRP1 marker.

Shuttle-vector system for S. cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins

Depositor: Dr. Hidekatsu Iha
Reference: Iha, H., Tsurugi, K. Biotechniques, 25, 936-938 (1998)., PMID 9863043.

Catalog no. Name Tag Ori Marker Promoter Size (kb) Usable sites
RDB01947 pAMH10 Myc centromere-type HIS3 ADH1 4.5 6
RDB01948 pAMH20 Myc 2micron HIS3 ADH1 5.3 6
RDB01949 pAML10 Myc centromere-type LEU2 ADH1 5.1 6
RDB01950 pAML20 Myc 2micron LEU2 ADH1 5.9 6
RDB01951 pAMT10 Myc centromere-type TRP1 ADH1 4.3 5
RDB01952 pAMT20 Myc 2micron TRP1 ADH1 5.1 5
RDB01953 pAMU10 Myc centromere-type URA3 ADH1 4.6 6
RDB01954 pAMU20 Myc 2micron URA3 ADH1 5.4 6
RDB01955 pGMH10 Myc centromere-type HIS3 GAL1-10 4.8 6
RDB01956 pGMH20 Myc 2micron HIS3 GAL1-10 5.6 6
RDB01957 pGML10 Myc centromere-type LEU2 GAL1-10 5.4 6
RDB01958 pGML20 Myc 2micron LEU2 GAL1-10 6.2 6
RDB01959 pGMT10 Myc centromere-type TRP1 GAL1-10 4.6 5
RDB01960 pGMT20 Myc 2micron TRP1 GAL1-10 5.4 5
RDB01961 pGMU10 Myc centromere-type URA3 GAL1-10 4.9 6
RDB01962 pGMU20 Myc 2micron URA3 GAL1-10 5.7 6

Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.


National BioResource Project – Yeast
Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.

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(GRP0047e 2008.05.20 T.M.)

2019.01.23