CRITGI, a new tool to control gene induction in budding yeast
CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner.
Hanasaki M, Masumoto H.
Sci. Rep. 9 (1): 15300 (2019). PubMed PMID 31653950.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB18135 | PHM661 | Integration plasmid to Ty1 loci, YIplac128 back bone (pTy1). CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18136 | PHM688 | Integration plasmid to Ty1 loci, pRS403 back bone. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18137 | PHM663 | Cas9 and sgRNA plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18138 | PHM664 | Cas9 and sgRNA #4 plasmid helping pTy1 integration into Ty1 loci. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18139 | PHM740 | Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18140 | PHM760 | Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18141 | PHM745 | Integration plasmid with TDH3 promoter to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18142 | PHM757 | Integration plasmid expressing histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18143 | PHM829 | Integration plasmid of histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18144 | PHM820 | Integration plasmid expressing Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18145 | PHM833 | Integration plasmid expressing indolepyruvate decarboxylase 1 (PDC1) to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18146 | PHM821 | Integration plasmid of histone H3 (HHT1) and Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
| RDB18147 | PHM802 | Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone. |
Saccharomyces cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli
Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals.
Iida, K., Teng, J., Tada, T., Saka, A., Tamai, M., Izumi-Nakaseko, H., Adachi-Akahane, S., Iida, H.
J. Biol. Chem. 282 (35): 25659-25667 (2007). PubMed PMID 17569661.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB15296 | pBC111 | S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli. |
Empty expression vector designed to produce GFP-tagged protein
Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1
Sasaki, T., Takagi, H.
Genes Cells, 18 (6): 459-475 (2013). PubMed PMID 23517290.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB08380 | pRS416-PADH1-ccdB-yEGFP-TTEF1 | Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria. |
| RDB08386 | pRS416-PADH1-yEGFP-TTEF1 | Empty expression vector backbone of S. cerevisiae |
Expression vector of S. cerevisiae Singlecopy plasmid
Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants.
Matsuda, N., Ueda, T., Sasaki, Y., Nakano, A.
Cell. Struct. Funct. 25 (1): 11-20 (2000). PubMed PMID 10791890.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB08655 | pSKY5 | Expression vector of S. cerevisiae. Single copy plasmid harboring the TDH3 promoter, EGFP, CMK1 terminator and the URA3 marker. |
| RDB08752 | pYO773 | Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the URA3 marker. |
| RDB08753 | pNMV3 | Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the HIS3 marker. |
| RDB08754 | pNMV4 | Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the TRP1 marker. |
Expression vector designed to produce C-terminal-Myc-tagged fusion proteins
Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins
Iha, H., Tsurugi, K.
Biotechniques, 25, 936-938 (1998). PubMed PMID 9863043.
| Catalog no. | Name | Tag | Ori | Marker | Promoter | Size (kb) | Usable sites |
|---|---|---|---|---|---|---|---|
| RDB01947 | pAMH10 | Myc | centromere-type | HIS3 | ADH1 | 4.5 | 6 |
| RDB01948 | pAMH20 | Myc | 2micron | HIS3 | ADH1 | 5.3 | 6 |
| RDB01949 | pAML10 | Myc | centromere-type | LEU2 | ADH1 | 5.1 | 6 |
| RDB01950 | pAML20 | Myc | 2micron | LEU2 | ADH1 | 5.9 | 6 |
| RDB01951 | pAMT10 | Myc | centromere-type | TRP1 | ADH1 | 4.3 | 5 |
| RDB01952 | pAMT20 | Myc | 2micron | TRP1 | ADH1 | 5.1 | 5 |
| RDB01953 | pAMU10 | Myc | centromere-type | URA3 | ADH1 | 4.6 | 6 |
| RDB01954 | pAMU20 | Myc | 2micron | URA3 | ADH1 | 5.4 | 6 |
| RDB01955 | pGMH10 | Myc | centromere-type | HIS3 | GAL1-10 | 4.8 | 6 |
| RDB01956 | pGMH20 | Myc | 2micron | HIS3 | GAL1-10 | 5.6 | 6 |
| RDB01957 | pGML10 | Myc | centromere-type | LEU2 | GAL1-10 | 5.4 | 6 |
| RDB01958 | pGML20 | Myc | 2micron | LEU2 | GAL1-10 | 6.2 | 6 |
| RDB01959 | pGMT10 | Myc | centromere-type | TRP1 | GAL1-10 | 4.6 | 5 |
| RDB01960 | pGMT20 | Myc | 2micron | TRP1 | GAL1-10 | 5.4 | 5 |
| RDB01961 | pGMU10 | Myc | centromere-type | URA3 | GAL1-10 | 4.9 | 6 |
| RDB01962 | pGMU20 | Myc | 2micron | URA3 | GAL1-10 | 5.7 | 6 |
Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.
National BioResource Project – Yeast
Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.
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2020.10.15