Saccharomyces cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli
Depositor: Dr. Hidetoshi Iida
Reference:
Cho, T. et al. Genes Cells 22 (1): 94-104 (2017). PMID:27935186.
Iida, K. et al. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB15296 | pBC111 | S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli. |
Empty expression vector backbone of S. cerevisiae to express GFP-tagged protein
Depositor: Dr. Hiroshi Takagi
Reference: Sasaki, T., Takagi, H. Genes Cells, 18 (6): 459-475 (2013). PMID 23517290.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB08380 | pRS416-PADH1-ccdB-yEGFP-TTEF1 | Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria. |
| RDB08386 | pRS416-PADH1-yEGFP-TTEF1 | Empty expression vector backbone of S. cerevisiae |
Expression vector of S. cerevisiae Singlecopy plasmid
Depositor: Dr. Akihiko Nakano
Reference: Sato, K., Sato, M., Nakano, A. J. Cell Biol. 152 (5): 935-944. PMID 11238450.
Reference: Matsuda, N., Ueda, T., Sasaki, Y., Nakano, A. Cell. Struct. Funct. 25 (1): 11-20 (2000). PMID 10791890.
| Catalog no. | Name | Running title |
|---|---|---|
| RDB08655 | pSKY5 | Expression vector of S. cerevisiae. Single copy plasmid harboring the TDH3 promoter, EGFP, CMK1 terminator and the URA3 marker. |
| RDB08752 | pYO773 | Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the URA3 marker. |
| RDB08753 | pNMV3 | Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the HIS3 marker. |
| RDB08754 | pNMV4 | Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the TRP1 marker. |
Shuttle-vector system for S. cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins
Depositor: Dr. Hidekatsu Iha
Reference: Iha, H., Tsurugi, K. Biotechniques, 25, 936-938 (1998)., PMID 9863043.
| Catalog no. | Name | Tag | Ori | Marker | Promoter | Size (kb) | Usable sites |
|---|---|---|---|---|---|---|---|
| RDB01947 | pAMH10 | Myc | centromere-type | HIS3 | ADH1 | 4.5 | 6 |
| RDB01948 | pAMH20 | Myc | 2micron | HIS3 | ADH1 | 5.3 | 6 |
| RDB01949 | pAML10 | Myc | centromere-type | LEU2 | ADH1 | 5.1 | 6 |
| RDB01950 | pAML20 | Myc | 2micron | LEU2 | ADH1 | 5.9 | 6 |
| RDB01951 | pAMT10 | Myc | centromere-type | TRP1 | ADH1 | 4.3 | 5 |
| RDB01952 | pAMT20 | Myc | 2micron | TRP1 | ADH1 | 5.1 | 5 |
| RDB01953 | pAMU10 | Myc | centromere-type | URA3 | ADH1 | 4.6 | 6 |
| RDB01954 | pAMU20 | Myc | 2micron | URA3 | ADH1 | 5.4 | 6 |
| RDB01955 | pGMH10 | Myc | centromere-type | HIS3 | GAL1-10 | 4.8 | 6 |
| RDB01956 | pGMH20 | Myc | 2micron | HIS3 | GAL1-10 | 5.6 | 6 |
| RDB01957 | pGML10 | Myc | centromere-type | LEU2 | GAL1-10 | 5.4 | 6 |
| RDB01958 | pGML20 | Myc | 2micron | LEU2 | GAL1-10 | 6.2 | 6 |
| RDB01959 | pGMT10 | Myc | centromere-type | TRP1 | GAL1-10 | 4.6 | 5 |
| RDB01960 | pGMT20 | Myc | 2micron | TRP1 | GAL1-10 | 5.4 | 5 |
| RDB01961 | pGMU10 | Myc | centromere-type | URA3 | GAL1-10 | 4.9 | 6 |
| RDB01962 | pGMU20 | Myc | 2micron | URA3 | GAL1-10 | 5.7 | 6 |
Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.
National BioResource Project – Yeast
Strains and plasmids of budding yeast (Saccharomyces cerevisiae) is available.
go to Head(GRP0047e 2008.05.20 T.M.)
2019.01.23