Thermus thermophilus HB8 Expression and Disruption plasmids were constructed by “Whole-Cell Project of a Model Organism, Thermus thermophilus HB8” and deposited by Dr. Seiki Kuramitsu.
Expression plasmid of Thermus thermophilus HB8 proteins in E. coli
Data Sheet
Resource name | Thermus thermophilus HB8 expression plasmid |
Vector | pET-11a (Ampr), pET-11b (Ampr) or pET-3a (Ampr) |
Insert | Genomic DNA of Thermus thermophilus strain HB8 |
Host | Plasmids were amplified using DH5alpha, E. coli |
Individual clone data | Available at Thermus thermophilus gene plasmid |
Reference |
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Related information | Please visit Informaion site for articles published by using this materials, references and tips. |
Disruption plasmid of Thermus Thermophilus HB8 gene
The gene disruptant of T. thermophils HB8 can be easily prepared by adding this plasmid into the culture medium. The target gene in this plasmid was replaced by the thermostable kanamycin resistant gene from Staphylococcus aureus. The length of the homologous region outside of the target gene is about 500 bp (only 10 bp of the target geneare left in both sides) .
Data Sheet
Resource name | Thermus thermophilus HB8 Disruption plasmid |
Vector | pGEM derivative (Ampr) |
Insert | Genomic DNA of Thermus thermophilus strain HB8, thermostable kanamycin resistant gene from Staphylococcus aureus |
Host | Plasmids were amplified using E. coli host strain for unstable inserts (ex. Stbl2) |
Individual clone data | Available at Thermus thermophilus gene plasmid |
Sequencing Primer |
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Related information | Please visit Informaion site for articles published by using this materials, references and tips. |
Disruption method
Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus.
Hoseki, J., Yano, T., Koyama, Y., Kuramitsu, S., Kagamiyama, H.
J. Biochem. 126 (5): 951-956 (1999). PubMed PMID 10544290.
Catalog no. | Name of resource | Description |
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RDB03436 | pUC18-HTK (with promoter) | Expression vector of Thermus highly thermostable kanamycin nucleotidyltransferase (HTK) gene |
Depositor: Dr. Jun Hoseki
Related article
- Molecular mechanisms of the whole DNA repair system: a comparison of bacterial and eukaryotic systems.
Shimada, A., Inoue, M., Iino, H., Wakamatsu, T., Fukui, K., Nakagawa, N., Masui, R., Kuramitsu, S. (2010)
J. Nucleic Acids 2010: 179594 - Increased Rigidity of Domain Structures Enhances the Stability of a Mutant Enzyme Created by Directed Evolution
Hoseki, J., Okamoto, A., Takada, N., Suenaga, A., Funatsugi, N., Konagaya, A., Taiji, M., Yano, T., Kuramitsu, S. and Kagamiyama, H. (2003)
Biochemistry 42, 14469-14475. - Disruption of Thermus thermophilus Genes by Homologous Recombination Using A Thermostable Kanamycin-Resistant Marker
Hashimoto, Y., Yano, T., Kuramitsu, S. and Kagamiyama, H. (2001)
FEBS Lett. 506, 231-234. - Directed Evolution of Thermostable Kanamycin-Resistant Gene Products in an Extremely Thermophilic Bacterium, Thermus thermophilus
Hoseki, J., Yano, T., Koyama, Y., Kuramitsu, S. and Kagamiyama, H. (1999)
J. Biochem. 126, 951-956.
Ordering information
Forms
Please specify ID (ex. TEx01A01, TDs01A01) of individual clone(s).
Please indicate Terms and Conditions for Distribution in MTA as follows:
(Yokoyama et al., 2000, Nature Struct. Biol. 7, 943-945).
Order Form: Please complete the form with your shipping information including your account number of an international courier (FedEx. World Courier, TNT Express, DHL Global Forwarding and others). See detail in Information of Request for Distribution Note: The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries |
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Order Form for Bank Transfer Payment. [Word] [Example of order form] |
Material Transfer Agreement (Category I MTA) [Word] For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.
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Send forms to:
The DNA Bank, RIKEN BioResource Research Center (BRC), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan E-mail: dna_sec.brc@riken.jp FAX: (+81)-29-836-9120 |
Please visit further information of distribution and fees.
Distribution fee per clone (as of April 1st, 2023): JPY 9,460 (For use in research for not-for-profit academic purpose). JPY 18,920 (For use in research for-profit-research purpose).
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TIPS
- Strain Thermus thermophilus HB8 (JCM 10941T) of BRC-JCM
- Genomic DNA (cat# JGD05989) of Thermus thermophilus HB8 (JCM 10941T)
(GRP0051e 2012.10.22 T.M.)
2023.06.08