Gene Engineering Division - DNA BANK - > Clone Set, Library & Genomic DNA > JCM Microbial Genomic DNA

JCM Microbial Genomic DNA

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General Information

Bacteria and archaeans are cultured in the RIKEN BRC-JCM and their DNA are prepared in the Gene Engineering Division. High molecular weight DNA is prepared by phenol-chloroform extraction.
 
The authenticity of microbial genomic DNAs for the distribution are confirmed by PCR amplification of 16S rRNA gene and nucleotide sequencing of the amplified products. However, these tests do not exclude contamination of any other species. Please be aware that the genomic DNA send from us may not necessarily fit for your specific research purpose.
For DNA from strains not on List of genomic DNA, please contact us.
 
Attention International Transfer – “Veterinary Permission” and/or “Permit to Import Quarantine Material” may be required for shipment of the biological materials. Please follow guide lines in your country.

Forms for Distribution

Order Form:
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
See detail in Information of Request for Distribution

Note:
The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries
Order Form for Credit Card Payment. (Visa or Master Card only) [Word] [Example of order form]
Order Form for Bank Transfer Payment. [Word] [Example of order form]

Material Transfer Agreement (Category I MTA) [Word]

For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.
  • Regarding Section 2(a):
    Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it.
    We can check whether the documents are filled out correctly or not in advance. Please email documents to us.
  • Regarding signature line:
    "Authorized Representative" is a person who is responsible for intellectual property rights. We request that the candidate is in one of the following positions, he/she can sign the MTA as the Authorized Representative. For anything unclear, please contact us by email.
    • College/University/Graduate School: President, Dean, Director or Head of Department
    • Research Institution: Director
    • Corporation: President, CEO, Director
    • Any officer appointed as Intellectual Property Administrator by the organization
    The MTA is a formal contract to be executed between institutions. Therefore, we ask your Authorized Representative to be an authority listed above.
Material Transfer Agreement (Category II MTA) [Word]

For the use of our bioresource in research for the following cases:
  • For research to be conducted by for-profit organizations.
  • For collaborative research between for-profit organization and not-for-profit organization.
  • For research by not-for- organization outsourced and sponsored by for-profit organization.
  • For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.

Please visit Information of Request for Distribution and Fee and Payment for further information.

  • If you are inquiring about the availability of a specific DNA, please give as much information about the strain stock number as possible.
  • The availability of some DNAs is limited. All requests for these DNAs will be given due consideration, but we cannot assure availability.
    For more information contact:
    The DNA Bank, RIKEN BioResource Research Center (BRC),
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    E-mail: dna_sec.brc@riken.jp

    For strains:
    Microbe Division / Japan Collection of Microorganisms, RIKEN BioResource Research Center (BRC),
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    E-mail: inquiry.jcm@riken.jp

Related Information

Reconstitution of DNA

  • In the case you received plastic vials contains precipitated DNA with ethanol.
  • A precipitant might stick to the lid or wall during transportation.
  • Before opening the tube, please spin down a minute or two at 5,000 g (~7,000 rpm, table top microfuge).
  • Please remove ethanol with pipet (DO NOT tooch precipitate!)
  • Immediately after removal of ethanol resuspend the DNA in an appropriate amount of TE buffer (10 mM Tris-Cl, pH 7.5, 1 mM EDTA), and dissolve the DNA pellet by gently tapping with fingers (DO NOT shake vigorously! A little amount of ethanol won’t affect to many applications).
  • If the DNA is difficult to solve, leave the vial at 4 degreeC overnight to allow the DNA to dissolve.
  • Once reconstituted in TE buffer, the DNA should be stored at -30 degreeC.
  • Please refer Genomic DNA Information for further information.

(GRP0005e 2017.06.30 T.M.)

2023.09.13



 

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