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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

BRC News

Genome Network Project Human cDNA Clones


Genome Network Project Human cDNA Clone

The RIKEN BRC is distributing copies of full-length human cDNA clones, produced and organized through the MEXT Genome Network Project to academia under its distribution policy.
There are two types of cDNA clone collections.
1) Human Full-Length cDNA clones
Approximately 30,000 clones corresponding to 60% of all human genes (14,000 genes) produced and organized by Dr. Sumio Sugano of the University of Tokyo and Dr. Yoshihide Hayashizaki of RIKEN OSC.
2) Human Gateway® Entry clones
Approximately 50,000 clones corresponding to 6,300 genes produced by cloning PCR-amplified open reading frame (ORF) fragments from the human full-length cDNA clones and studied through the government-supported MEXT Genome Network Project. DNA fragments cloned into Gateway® entry clones can be transferred into one or more destination vectors simultaneously using Gateway® technology. The Gateway® Entry clones include clones made from cDNA created by the Research Association for Biotechnology (RAB) under the New Energy and Industrial Technology Development Organization (NEDO) Full-length Human cDNA Sequencing Project.

Results of the project attained using the Genome Network Project cDNA clones have appeared in journals including Nature Genetics. Detailed information on these clones is available via the Genome Network Platform.

References:

The papers describing these libraries have been published in journals indicated below:

  1. Ota T. et al., Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36: 40-45, 2004.
  2. Otsuki T. et al., Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res., 12: 117-126, 2005.
  3. Kimura K. et al., Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16: 55-65, 2006.
  4. Itoh M., Yasunishi A., Imamura K., Kanamori M., Suzuki H., Suzuki M., Carninci P., Kawai J., Hayashizaki Y. Constructing ORFeome resources with removable termination codons, Biotechniques, 41: 44-48, 2006.

 

  • For more information about Gateway® technology, please visit the website of Invitrogen.
  • Gateway® is a registered trademark of Invitrogen.
  • Please visit the website of the Biological Resource Center (NBRC) at the National Institute of Technology and Evaluation (NITE) for more information about cDNA of the NEDO Full-length Human cDNA Sequencing Project.

Please visit Informaion site for articles published by using this materials, references and tips.

Clone search

  • site search
  • Browse by A to Z list
    • Browse your interesting gene by A to Z list sorted by locus symbol.
  • Search by GNP Viewer

In the case of Full-Length Clone

  • Full-Length Clone(s) is indicated as “IRxxxxxxxx” in the “Clone:” column.
  • Link of the insert sequence is indicated in the “Accession:” column. Please make sure if or not your desired sequence is included.
  • The name of vector appears in “Vector:”. Please confirm the name of vector. In the case of Gateway® Expression Clone (pCMV SPORT6 and related vectors), users are asked to pay RIKEN BRC an additional 1,250 YEN per clone that includes the license fee, handling of currency exchange, money transfer and other expense.

In the case of Entry Clone

  • Entry Clone(s) is indicated as “W01xxxxxxx” and “M01xxxxxxx” in the “Clone:”.
  • Link of the parental sequence but not the Entry Clone is indicated in the “Accession” column. Please e-mail us for a junction of insert-vector sequence of Entry Clone.
  • The name of vector appears in “Vector:”. Please confirm the name of vector.

Ordering Genome Network Project Clones

in Japanese
Terms and Conditions for distribution:
The BIOLOGICAL RESOURCE is provided only for academic research in the non-profit organization

  1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures designated by the Research Association for Biotechnology, Dr. Yoshihide Hayashizaki and Dr. Sumio Sugano is requested.:
    • Ota T. et al., Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36: 40-45, 2004.
    • Otsuki T. et al., Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res., 12: 117-126, 2005.
    • Kimura K. et al., Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16: 55-65, 2006.
    • Itoh M., Yasunishi A., Imamura K., Kanamori M., Suzuki H., Suzuki M., Carninci P., Kawai J., Hayashizaki Y. Constructing ORFeome resources with removable termination codons, Biotechniques, 41: 44-48, 2006.
  2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Research Association for Biotechnology, Dr. Yoshihide Hayashizaki of RIKEN and Dr. Sumio Sugano of Tokyo University is requested.
  3. The Research Association for Biotechnology does not warrant that the use of the BIOLOGICAL RESOURCE by the RECIPIENT will not infringe any patent, copyright, trademark or other intellectual property rights of third parties.
  4. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

Distribution fee per clone:
8,440 YEN (For use in research for not-for-profit academic purpose).
(Shipping cost is not included.)

For details for fee and payment, please visit Information of Request for Distribution.
The distribution fee was revised from October 1, 2019

In the case of Gateway® Expression Clone (pCMV SPORT6 and related vectors), users are asked to pay RIKEN BRC an additional 1,250 YEN per clone that includes the license fee, handling of currency exchange, money transfer and other expense.
Information for the license fee
Please reffer Data Sheet for the backbone vectors.

Form:
DNA solution with TE buffer (approx. 1 microgram) per clone.
Remarks:
Terminal nucleotide sequences of cDNA are checked prior to delivery.
Please allow 2 weeks before shipment.

If you have any questions regarding our DNA resources or related matters, please feel free to contact the Gene Engineering Division.

Order Forms and Material Transfer Agreement (MTA)

Obtain FormA.

Order Form:
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
See detail in Information of Request for Distribution

Note:
The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries
For Credit Card Payment. (Visa or Master Card only)
Order Form (Credit Card Payment) 
For bank transfer or check payment.
Order Form (Bank Transfer or Check Payment) 

Forms Description
Form C Material Transfer Agreement [Doc] (For use for not-for-profit academic purpose) We request a signature of an authorized representative of a recipient institution. In the the Section 2(a), please write your purpose of use of clone in 10 to 20 words.
Limited Use Label License: No.19 (Gateway® Cloning Products) pdf Please download and read the Ordering Form and Label License No. 19.

Address for orders

Please complete one copy of Form A and two copies of Form C and send them to the Gene Engineering Division by post or e-mail. In case sending them by e-mail, please send us the originals by post afterwards.
We look forward to receiving your order.

The DNA Bank, RIKEN BioResource Research Center,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dnabank.brc@riken.jp
FAX: (+81)-29-836-9120

 

Payment and charges

An INVOICE will be sent to you or your designated billing address. Prompt payment will be most appreciated. Please refer to the following page for more details: Information of Request for Distribution
RIKEN requests all applicants to cover all bank charges, (including exchange, commission and handling fees) incurred in sending payment for resources you have ordered.

Notice of Citations

When publishing, in scientific journals, the results of research involving the use of materials from RIKEN BRC, please be sure to cite the paper designated by the depositor as well as to mention that these materials were provided by RIKEN BRC as follows: (name of BIOLOGICAL RESOURCE) was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan.

To contact us

The DNA Bank, RIKEN BioResource Research Center,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dnabank.brc@riken.jp
FAX: (+81)-29-836-9120

Announcement

Life Technologies Corporation signs license agreement with RIKEN BRC

  • The Life Technologies Corporation (formerly Invitrogen IP Holdings, Inc.) and the RIKEN BRC signed a license agreement on July 2, 2009. Thanks to the generosity of the Life Technologies Corporation, the RIKEN BRC is now able, under this agreement, to receive, maintain, replicate and distribute Gateway® Entry clones and Expression clones for not-for-profit academic research.(more…)

(Gateway® is a registered trademark of Invitrogen.)

data sheet

Nucleotide Sequences of Vectors

For maps, lease visit the Vector Information and Library Method Overviews in the MGC.

Vector (link to the PlasmID Repository) Seq File & Primers
pBluescriptR [link] Primer A: T7 GTAATACGACTCACTATAGGGC
Primer B: Reverse2 GCGGATAACAATTTCACACAGG
pCMV-SPORT6 [link] Primer A: Reverse2 GCGGATAACAATTTCACACAGG
Primer B: M13 GTTTTCCCAGTCACGACGTTGTA
Primer C: TTTTTTTTTTTTTTTTTTTTNNN
pCMV-SPORT6.1 [link] Primer A: Reverse2 GCGGATAACAATTTCACACAGG
Primer B: M13 GTTTTCCCAGTCACGACGTTGTA
Primer C: TTTTTTTTTTTTTTTTTTTTNNN
pCMV-SPORT6.ccdb [link] Primer A: Reverse2 GCGGATAACAATTTCACACAGG
Primer B: M13 GTTTTCCCAGTCACGACGTTGTA
Primer C: TTTTTTTTTTTTTTTTTTTTNNN
pCR4-TOPO [link] Primer A: M13 GTTTTCCCAGTCACGACGTTGTA
Primer B: Reverse2 GCGGATAACAATTTCACACAGG
pDONR221 [link] Primer A: M13 GTTTTCCCAGTCACGACGTTGTA
Primer B: T7 TAATACGACTCACTATAGGG
pDNR-Dual [link]
pDNR-LIB [link] Primer A: T7 TAATACGACTCACTATAGGG
Primer B: M13 Reverse AAACAGCTATGACCATGTTCA
pENTR/D-TOPO [link] Primer A: M13 GTTTTCCCAGTCACGACGTTGTA
Primer B: T7 TAATACGACTCACTATAGGG
pOTB7 (pBR322 ori) [link] Primer A: pOTB7_F(7-32) AACGCGGCTACAATTAATACATAACC
Primer B: pOTB7_R(358-335) GTACTGCAGCCGATTCATTAATGC
Primer C: TTTTTTTTTTTTTTTTTTTTNNN

 

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(GRP0032e 2010.12.02 T.M.)

2019.08.12