Genome Network Project Human cDNA Clones | Gene Engineering Division (RIKEN BRC)

Genome Network Project Human cDNA Clone

The RIKEN BioResource Research Center (BRC) is distributing copies of full-length human cDNA clones, produced and organized through the MEXT Genome Network Project to academia under its distribution policy.
There are two types of cDNA clone collections.
1) Human Full-Length cDNA clones
Approximately 30,000 clones corresponding to 60% of all human genes (14,000 genes) produced and organized by Dr. Sumio Sugano of the University of Tokyo and Dr. Yoshihide Hayashizaki of RIKEN OSC.
2) Human Gateway® Entry clones
Approximately 50,000 clones corresponding to 6,300 genes produced by cloning PCR-amplified open reading frame (ORF) fragments from the human full-length cDNA clones and studied through the government-supported MEXT Genome Network Project. DNA fragments cloned into Gateway® entry clones can be transferred into one or more destination vectors simultaneously using Gateway® technology. The Gateway® Entry clones include clones made from cDNA created by the Research Association for Biotechnology (RAB) under the New Energy and Industrial Technology Development Organization (NEDO) Full-length Human cDNA Sequencing Project.

Results of the project attained using the Genome Network Project cDNA clones have appeared in journals including Nature Genetics. Detailed information on these clones is available via the Genome Network Platform.

References:

The papers describing these libraries have been published in journals indicated below:

Ota T. et al., Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36: 40-45, 2004.
Otsuki T. et al., Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res., 12: 117-126, 2005.
Kimura K. et al., Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16: 55-65, 2006.
Itoh M., Yasunishi A., Imamura K., Kanamori M., Suzuki H., Suzuki M., Carninci P., Kawai J., Hayashizaki Y. Constructing ORFeome resources with removable termination codons, Biotechniques, 41: 44-48, 2006.

For more information about Gateway® technology, please visit the website of Invitrogen.
Gateway® is a registered trademark of Invitrogen.
Please visit the website of the Biological Resource Center (NBRC) at the National Institute of Technology and Evaluation (NITE) for more information about cDNA of the NEDO Full-length Human cDNA Sequencing Project.
Please visit Japan Biological Informatics Consortium (JBIC) regarding the NEDO Full-length Human cDNA provided by NITE.

Please visit Informaion site for articles published by using this materials, references and tips.
Information for the license fee
Please reffer Data Sheet for the backbone vectors.

Clone search

Key word search

Browse by A to Z list

Browse your interesting gene by A to Z list sorted by locus symbol.

How to distinguish between Full-length and Entry clones

Full-Length Clone(s) is indicated as “IRxxxxxxxx” in the “Clone name” line.
- Link of the insert sequence is indicated in the “Sequence, submitted” line. Please make sure if or not your desired sequence is included.
- Please confirm the name of vector in “Vector” line. In the case of Gateway® Expression Clone (pCMV SPORT6 and related vectors), users are asked to pay BRC an additional 1,250 YEN per clone that includes the license fee, handling of currency exchange, money transfer and other expense.
Entry Clone(s) is indicated as “W01xxxxxxx” and “M01xxxxxxx” in the “Clone name” line.
- Link of the parental sequence but not the Entry Clone is indicated in the “Accession” column. Please e-mail us for a junction of insert-vector sequence of Entry Clone.

Ordering Genome Network Project Clones

in Japanese
The BIOLOGICAL RESOURCE is provided only for academic research in the non-profit organization

Terms and Conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures designated by the Research Association for Biotechnology, Dr. Yoshihide Hayashizaki and Dr. Sumio Sugano is requested.:
- Ota T. et al., Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36: 40-45, 2004.
- Otsuki T. et al., Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res., 12: 117-126, 2005.
- Kimura K. et al., Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16: 55-65, 2006.
- Itoh M., Yasunishi A., Imamura K., Kanamori M., Suzuki H., Suzuki M., Carninci P., Kawai J., Hayashizaki Y. Constructing ORFeome resources with removable termination codons, Biotechniques, 41: 44-48, 2006.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Research Association for Biotechnology, Dr. Yoshihide Hayashizaki of RIKEN and Dr. Sumio Sugano of Tokyo University is requested.
The Research Association for Biotechnology does not warrant that the use of the BIOLOGICAL RESOURCE by the RECIPIENT will not infringe any patent, copyright, trademark or other intellectual property rights of third parties.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

Distribution Fees

Distribution fee per clone (as of April 1st, 2023):
JPY 9,460 (For use in research for not-for-profit academic purpose).

The cost of shipping containers, dry ice (if required) and freight will be charged separately. These are not included in the fee.
For details for fee and payment, please visit Information of Request for Distribution.

In the case of Gateway® Expression Clone (pCMV SPORT6 and related vectors), users are asked to pay BRC an additional 1,250 YEN per clone that includes the license fee, handling of currency exchange, money transfer and other expense.

Forms

Order Form

Order Form:
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
See detail in Information of Request for Distribution

Note:
The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries

Order Form for Credit Card Payment. (Visa or Master Card only) [Word]

Order Form for Bank Transfer Payment. [Word]

Material Transfer Agreement (MTA)

Forms	Description
Form C Material Transfer Agreement [Docx] (For use for not-for-profit academic purpose)	For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization. Regarding Section 2(a): Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it. We can check whether the documents are filled out correctly or not in advance. Please email documents to us. Regarding signature line: “Authorized Representative” is a person who is responsible for intellectual property rights. We request that the candidate is in one of the following positions, he/she can sign the MTA as the Authorized Representative. For anything unclear, please contact us by email. College/University/Graduate School: President, Dean, Director or Head of Department Research Institution: Director Corporation: President, CEO, Director Any officer appointed as Intellectual Property Administrator by the organization The MTA is a formal contract to be executed between institutions. Therefore, we ask your Authorized Representative to be an authority listed above.
Limited Use Label License: No.19 (Gateway® Cloning Products) pdf	Please download and read the Ordering Form and Label License No. 19.

Address for orders

Please complete one copy of Form A and two copies of Form C and send them to the Gene Engineering Division by post or e-mail. In case sending them by e-mail, please send us the originals by post afterwards.
We look forward to receiving your order.

The DNA Bank, RIKEN BioResource Research Center (BRC),
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dna_sec.brc@riken.jp
FAX: (+81)-29-836-9120

Payment and charges

An INVOICE will be sent to you or your designated billing address. Prompt payment will be most appreciated. Please refer to the following page for more details: Information of Request for Distribution
RIKEN requests all applicants to cover all bank charges, (including exchange, commission and handling fees) incurred in sending payment for resources you have ordered.

Shipment

Form:
DNA solution with TE buffer (approx. 1 microgram) per clone.
Remarks:
Terminal nucleotide sequences of cDNA are checked prior to delivery.
Please allow 2 weeks before shipment.

If you have any questions regarding our DNA resources or related matters, please feel free to contact the Gene Engineering Division.

Notice

Citation

When publishing, in scientific journals, the results of research involving the use of materials from RIKEN BRC, please be sure to cite the paper designated by the depositor as well as to mention that these materials were provided by RIKEN BRC as follows: (name of BIOLOGICAL RESOURCE) was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan.

To contact us

The DNA Bank, RIKEN BioResource Research Center (BRC),
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dna_sec.brc@riken.jp
FAX: (+81)-29-836-9120

Data Sheet

Primers for sequencing vectors

Vectors	Primers
pBluescriptR	Insert 5′: T7 (Pr0202) GTAATACGACTCACTATAGGGC Insert 3′: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG
pCMV-SPORT6	Insert 5′: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG Insert 3′: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA Upstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN (V: mixture of A,G and C)
pCMV-SPORT6.1	Insert 5′: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG Insert 3′: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA Upstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN (V: mixture of A,G and C)
pCMV-SPORT6.ccdb	Insert 5′: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG Insert 3′: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA Upstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN (V: mixture of A,G and C)
pCR4-TOPO	Insert 5′: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA Insert 3′: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG
pDONR221	Insert 5′: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA Insert 3′:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG
pDNR-Dual	Insert 5′:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG Insert 3′:Dual-R (Pr0072) ATCCAAGCACTAGTCATGAC
pDNR-LIB	Insert 5′:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG Insert 3′: M13 Reverse (Pr0042) AAACAGCTATGACCATGTTCA
pENTR/D-TOPO	Insert 5′: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA Insert 3′:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG
pOTB7 (pBR322 ori)	Insert 5′: pOTB7_F (Pr0224) AACGCGGCTACAATTAATACATAACC Insert 3′: pOTB7_R (Pr0225) GTACTGCAGCCGATTCATTAATGC Upstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN (V: mixture of A,G and C)

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2023.09.06