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BRC News

Ciona intestinalis EST Clones

General Information

Ciona intestinalis EST Clones were deposited by Dr. Noriyuki Satoh, Facuity of Science Graduate School of Science, Kyoto University; Individual clones are available. This set is known as the “Ciona intestinalis Gene Collection Release I” (Satou, Y. et al., 2002, Genesis 33 (4): 153-154; PMID, 12203911).

Library information:

Library ID Library Clone ID vector
CiEG Egg cieg001a01-cieg101p24 pBluescriptII SK(-)
CiCL Cleaving embryo cicl001a01-cicl022p24
pBluescriptII SK(-)
CiGN Gastruia/Neuruia cign001a01-cign081p24 pBluescriptII SK(-)
CiTB Tailbud embryo citb001a01-citb105p24 pBluescriptII SK(-)
CiLV Larva cilv001a01-cilv090p24 pBluescriptII SK(-)
CiAD Young aduit ciad001a01-ciad020p24
pBluescriptII SK(-)
CiGD Gonad cigd001a01-cigd049p24 pBluescriptII SK(-)
CiTS Testis cits001a01-cits050p24 pBluescriptII SK(-)
CiES Endostyle cies001a01-cies009p24 pBluescriptII SK(-)
CiNC Neural complex cinc001a01-cinc034p24 pBluescriptII SK(-)
CiHT Heart ciht001a01-ciht042p24 pBluescriptII SK(-)
CiBD Blood cells cibd001a01-cibd085p24 pBluescriptII SK(-)
CiEM Embryo mix ciem801a01-ciem854p24 pDONR222
CiJV Juvenile cijv001a01-cijv051p24
pBluescriptII SK(-)
CiDG Digestive Gland cidg001a01-cidg007p24
CiMA Mature aduit cima001a01-cima041p24
pBluescriptII SK(-)
CiMA Mature aduit cima801a01-cima854p24 pDONR222

The papers describing these libraries have been published in journals indicated below:

  1. Jeffery, W.R., Chiba, T., Krajka, F.R., Deyts, C., Satoh, N., Joly, J.S., 2008, Trunk lateral cells are neural crest-like cells in the ascidian Ciona intestinalis: insights into the ancestry and evolution of the neural crest. Dev. Biol., 324 (1): 152-160. PMID: 18801357.
  2. Imai, K.S., Hino, K., Yagi, K., Satoh, N., Satou, Y., 2004, Gene expression profiles of transcription factors and signaling molecuies in the ascidian embryo: towards a comprehensive understanding of gene networks. Development, 131 (16): 4047-4058. PMID: 15269171.
  3. Azumi, K., Takahashi, H., Miki, Y., Fujie, M., Usami, T., Ishikawa, H., Kitayama, A., Satou, Y., Ueno, N., Satoh, N., 2003, Construction of a cDNA microarray derived from the ascidian Ciona intestinalis. Zoolog. Sci. 20 (10): 1223-1229. PMID: 14569145.
  4. Satou, Y., Yamada, L., Mochizuki, Y., Takatori, N., Kawashima, T., Sasaki, A., Hamaguchi, M., Awazu, S., Yagi, K., Sasakura, Y., Nakayama, A., Ishikawa, H., Inaba, K., Satoh, N., 2002, A cDNA resource from the basal chordate Ciona intestinalis. Genesis, 33 (4): 153-154. PMID: 12203911.
  5. Embryonic retinoic acid target genes in the ascidian Ciona intestinalis web site
  6. The Ghost Database web site

Please visit Informaion site for articles published by using this materials, references and tips.

Ordering Ciona intestinalis EST Clones

Terms and Conditions for Distribution

  1. In publishing their resuits in any kind of publication types, RECIPIENT refers to the Ghost Home ( genomic and cDNA resources ) web site
    ( and DEPOSITOR’s related publications,
  2. Sending the DEPOSITOR a reprint or equivalent of RECIPIENT’s publication after publishing their resuits,
  3. Using the clones only for academic purpose, and
  4. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT’s name and their affiliation.

   Uses other than for academic research by non-profit institutions:
   Please contact the Gene Engineering Division.

Distribution fee per clone:
8,440 YEN (For use in research for not-for-profit academic purpose).
(Shipping cost is not included.)

For details for fee and payment, please visit Information of Request for Distribution.
The distribution fee was revised from October 1, 2019

DNA solution with TE buffer (approx. 1 microgram) per clone.

♦ Terminal nucleotide sequences of cDNA are checked prior to delivery.
♦ Please allow 2 weeks before shipment.

If you have any questions regarding our DNA resources or related matters, please feel free to contact the Gene Engineering Division.

Clone search

Please find ID of individual clone(s) using the following sites.

Order Forms and Material Transfer Agreement (MTA)

Order Form:
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
See detail in Information of Request for Distribution

The resident of the European Economic Area (EEA) and China, please read Special distribution Information to Residents of the Foreign Countries
For Credit Card Payment. (Visa or Master Card only)
Order Form (Credit Card Payment) 
For bank transfer or check payment.
Order Form (Bank Transfer or Check Payment) 

Material Transfer Agreement (Category I MTA) [Word]
For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.

  • We ask a signature of the Authorized Representative of a recipient institution.
    In the Section 2(a), please write your purpose of use of clone in 10 to 20 words.
  • Please ask us if applicable for the following use:
    • For research to be conducted by for-profit organizations.
    • For collaborative research between for-profit organization and not-for-profit organization.
    • For research by not-for- organization outsourced and sponsored by for-profit organization.
    • For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.

Address for orders

Please complete one copy of Form A and two copies of Form C and send them to the Gene Engineering Division by post or e-mail. In case sending them by e-mail, please send us the originals by post afterwards.
We look forward to receiving your order.

The DNA Bank, RIKEN BioResource Research Center,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
FAX: (+81)-29-836-9120

Payment and charges

An INVOICE will be sent to you once you have received the requested materials.
Please refer to the following page for more details:
Information of Request for Distribution.
RIKEN is a non-profit organization and therefore requires all applicants to cover all bank charges, (including exchange, commission and handling fees) incurred in sending payment for resources they have ordered.

Notice of Citations

♦ When publishing, in scientific journals, the resuits of research involving the use of materials from RIKEN BRC, please be sure to cite the paper designated by the depositor as well as to mention that these materials were provided by RIKEN BRC as follows:(name of BIOLOGICAL RESOURCE) was provided by the RIKEN BRC which is participating in the MEXT National BioResource Project (Japan).

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(2011.07.25 T.M.)