Optogenetics resource
Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation.
Ueda, Y., Miura, Y., Tomishige, N., Sugimoto, N., Murase, M., Kawamura, G., Sasaki, N., Ishiwata, T., Ozawa, T.
Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PubMed PMID 36288730.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB20343 | Lenti-pCSII-CMV-PPAP2.1-IRES-puro(Amp) | Lentivirus vector plasmid. To visualize half of the PPAP2 moieties, the mVenus fluorescent proteins were fused to LOV2-SsrA (PPAP2.1). |
| DNA resource | RDB20663 | Lenti-pCSII-CMV-PPAP2.2-IRES-puro(Amp) | Lentivirus vector plasmid. To visualize half of the PPAP2 moieties, the mCherry fluorescent proteins were fused to iSH2-SspB (PPAP2.2). |
| DNA resource | RDB20664 | pCAG-His-iSH-SspB-P2AT2A-mVenus-iLID-CAAX | Expression vector of PPAP2.1. |
| DNA resource | RDB20665 | pCAGkawa-His-iSH-SspB-mCherry-P2AT2A-iLID-CAAX | Expression vector of PPAP2.2. |
Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3.
Imayoshi, I., Tabuchi, S., Matsumoto, M., Kitano, S., Miyachi, H., Kageyama, R., Yamanaka, A.
Sci. Rep. 10 (1): 3191 (2020). PubMed PMID 32081938.
Mouse resource
| Category | Catalog # | Name of resource |
|---|---|---|
| Mouse resource | RBRC05159 | B6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR3/EYFP)Imayo> |
| Mouse resource | RBRC05160 | B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR3/EYFP)Imayo> |
| Mouse resource | RBRC05161 | B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR3/EYFP)Imayo> |
| Mouse resource | RBRC06583 | C57BL/6-Tg(Gt(ROSA)26Sor-eNpHR3/EYFP)1Imayo |
| Mouse resource | RBRC06584 | C57BL/6-Tg(Gt(ROSA)26Sor-eNpHR3/EYFP)1.1Imayo |
Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval.
Nguyen, R., Venkatesan, S., Binko, M., Bang, J.Y., Cajanding, J.D., Briggs, C., Sargin, D., Imayoshi, I., Lambe, E.K., Kim, J.C.
J. Neurosci. 40 (11): 2314-2331 (2020). PubMed PMID 32005764
Mouse resource
| Category | Catalog # | Name of resource |
|---|---|---|
| Mouse resource | RBRC06585 | C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1Imayo |
| Mouse resource | RBRC06586 | C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.1Imayo |
| Mouse resource | RBRC06587 | C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.2Imayo |
Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light.
Asano, T., Igarashi, H., Ishizuka, T., Yawo, H.
Front. Neurosci. 12: 561 (2018). PubMed PMID 30174581.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16748 | pCAGGS-ChRGR-Venus | Expression vector of organelle optogenetics channelrhodopsin. |
| DNA resource | RDB16750 | pCAGGS-ChRGR(ER)-Venus | Expression vector of organelle optogenetics channelrhodopsin. |
Expression vector of the channelrhodopsin-green receiver (ChRGR) (cat# RDB16748) which induces membrane potential oscillation by green light irradiation.
Expression vector of the ChRGR(ER) (cat# RDB16750) which localizes ChRGR to endoplasmic/sarcoplasmic reticulum (ER/SR). ChRGR(ER) is used as a tool to manipulation of intracellular calcium ion release.
A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer.
Hoque, M.R., Ishizuka, T., Inoue, K., Abe-Yoshizumi, R., Igarashi, H., Mishima, T., Kandori, H., Yawo, H.
PLoS One 11 (11): e0166820 (2016). PubMed PMID 27861619.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16777 | pCMV-KR2-3.0-EYFP | Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16778 | pCaMKIIα-KR2-3.0-EYFP-WPRE | Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CaMKIIα promoter. |
| DNA resource | RDB16779 | pCMV-IaNaR-3.0-EYFP | Mammalian expression vector encoding a light-driven sodiun ion pump from Indibacter alkaliphilus (IaNaR) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16780 | pCMV-I1K6NaR-3.0-EYFP | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16781 | pCMV-I2K5NaR-3.0-EYFP | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16782 | pCMV-I3K4NaR-3.0-EYFP | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16783 | pCMV-I4K3NaR-3.0-EYFP | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16784 | pCMV-I5K2NaR-3.0-EYFP | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16785 | pCMV-I6K1NaR-3.0-EYFP | Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter. |
The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1).
Watanabe, S., Ishizuka, T., Hososhima, S., Zamani, A., Hoque, M.R., Yawo, H.
Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PubMed PMID 26853505.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16786 | pCMV-MvChR1-Venus | Mammalian expression vector encoding Met1-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1) tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16787 | pCMV-eMvChR1#1-Venus | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#1) tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16788 | pCMV-eMvChR1#2-Venus | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16789 | pCMV-eMvChR1#2(A97D)-Venus | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97D mutation was introduced. |
| DNA resource | RDB16790 | pCMV-eMvChR1#2(A97E)-Venus | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97E mutation was introduced. |
| DNA resource | RDB16791 | pCMV-eMvChR1#2(A97Q)-Venus | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97Q mutation was introduced. |
| DNA resource | RDB16792 | pCMV-eMvChR1#2(A97R)-Venus | Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97R mutation was introduced. |
Near-infrared (NIR) up-conversion optogenetics.
Hososhima, S., Yuasa, H., Ishizuka, T., Hoque, M.R., Yamashita, T., Yamanaka, A., Sugano, E., Tomita, H., Yawo, H.
Sci. Rep. 5: 16533 (2015). PubMed PMID 26552717.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16793 | pCMV-C1V1-Venus | Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16794 | pCMV-PsChR-Venus | Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16795 | pCaMKIIα-C1V1-Venus | Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CaMKIIa promoter. |
| DNA resource | RDB16796 | pCaMKIIα-PsChR-Venus | Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CaMKIIa promoter. |
Structural basis for Na(+) transport mechanism by a light-driven Na(+) pump.
Kato, H.E., Inoue, K., Abe-Yoshizumi, R., Kato, Y., Ono, H., Konno, M., Hososhima, S., Ishizuka, T., Hoque, M.R., Kunitomo, H., Ito, J., Yoshizawa, S., Yamashita, K., Takemoto, M., Nishizawa, T., Taniguchi, R., Kogure, K., Maturana, A.D., Iino, Y., Yawo, H., Ishitani, R., Kandori, H., Nureki, O.
Nature 521 (7550): 48-53 (2015). PubMed PMID 25849775.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16777 | pCMV-KR2-3.0-EYFP | Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter. |
| DNA resource | RDB16778 | pCaMKIIα-KR2-3.0-EYFP-WPRE | Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CaMKIIa promoter. |
Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation.
Tsunematsu, T., Ueno, T., Tabuchi, S., Inutsuka, A., Tanaka, K.F., Hasuwa, H., Kilduff, T.S., Terao, A., Yamanaka, A.
J. Neurosci. 34 (20): 6896-6909 (2014). PubMed PMID 24828644.
Mouse resource
| Category | Catalog # | Name of resource |
|---|---|---|
| Mouse resource | RBRC05843 | B6.Cg-Actb<tm1(tetO-ChR2*E123T/T159C)Ahky> |
See also
December 2013 Mouse of the Month. New reporter line for optogenetic stimulation at high frequencies
Long-lasting silencing of orexin/hypocretin neurons using archaerhodopsin induces slow-wave sleep in mice.
Tsunematsu, T., Tabuchi, S., Tanaka, K.F., Boyden, E.S., Tominaga, M., Yamanaka, A.
Behav. Brain Res. 255: 64-74 (2013). PubMed PMID 23707248.
Mouse resource
| Category | Catalog # | Name of resource |
|---|---|---|
| Mouse resource | RBRC05842 | C57BL/6-Tg(tetO-ArchT/EGFP)1Ahky |
Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system.
Tanaka, K.F., Matsui, K., Sasaki, T., Sano, H., Sugio, S., Fan, K., Hen, R., Nakai, J., Yanagawa, Y., Hasuwa, H., Okabe, M., Deisseroth, K., Ikenaka, K., Yamanaka, A.
Cell Rep. 2 (2): 397-406 (2012). PubMed PMID 22854021.
Mouse resource
| Category | Catalog # | Name of resource |
|---|---|---|
| Mouse resource | RBRC05453 | B6(Cg)-Tg(tetO-ChR2*C128S/EYFP)1Kftnk |
| Mouse resource | RBRC05454 | B6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk> |
See also
April 2013 Mouse of the MonthExpanding the repertoire of optogenetic control
Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0.
Imayoshi, I., Tabuchi, S., Hirano, K., Sakamoto, M., Kitano, S., Miyachi, H., Yamanaka, A., Kageyama, R.
Neurosci. Res. 75 (1): 53-58 (2013). PubMed PMID 22465523.
Mouse resource
| Category | Catalog # | Name of resource |
|---|---|---|
| Mouse resource | RBRC05150 | B6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR2/EYFP)Imayo> |
| Mouse resource | RBRC05151 | B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2/EYFP)Imayo> |
| Mouse resource | RBRC05152 | B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo> |
Optically controlled contraction of photosensitive skeletal muscle cells.
Asano, T., Ishizua, T., Yawo, H..
Biotechnolm Bioeng. 2012 109(1): 199-204 (2012). PubMed PMID 21809334.
Cell resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| Cell resource | RCB4693 | ChR2-C2C12 | C2C12 cells stably expressing ChR2 |
Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin.
Wen, L., Wang, H., Tanimoto, S., Egawa, R., Matsuzaka, Y., Mushiake, H., Ishizuka, T., Yawo, H.
PLoS One 5 (9): e12893 (2010). PubMed PMID 20886118.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16775 | pCMV-chopGR-Venus | Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16776 | pCMV-ChRGR-Venus | Mammalian expression vector encoding a mammalian codon-optimized chopGR tagged with Venus at the C-terminus under CMV promoter. |
Molecular determinants differentiating photocurrent properties of two channelrhodopsins from Chlamydomonas.
Wang, H., Sugiyama, Y., Hikima, T., Sugano, E., Tomita, H., Takahashi, T., Ishizuka, T., Yawo, H.
J. Biol. Chem. 284 (9): 5685-5696 (2009). PubMed PMID 19103605.
DNA resource
| Category | Catalog # | Name of resource | description |
|---|---|---|---|
| DNA resource | RDB16769 | pCMV-chop1-Venus | Mammalian expression vector encoding channelopsin-1 (ChR1) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16770 | pCMV-chop2-Venus | Mammalian expression vector encoding channelopsin-2 (ChR2) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16771 | pCMV-chopFR-Venus | Mammalian expression vector encoding a chimeric channelopsin (channelopsin/fast-receiver, chopFR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16772 | pCMV-chopWR-Venus | Mammalian expression vector encoding a chimeric channelopsin (channelopsin/wide-receiver, chopWR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16773 | pCMV-ChRFR-Venus | Mammalian expression vector encoding a mammalian codon-optimized chopFR tagged with Venus at the C-terminus under CMV promoter. |
| DNA resource | RDB16774 | pCMV-ChRWR-Venus | Mammalian expression vector encoding a mammalian codon-optimized chopWR tagged with Venus at the C-terminus under CMV promoter. |
2025.05.03 (T.M. GRP0057e)
