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Expression Vector Backbone of S. pombe


Empty expression vector backbone of Schizosaccharomyces pombe

Reference: PLoS One 9, e112488 (2014)., PMID 25401760.

Catalog No. plasmid backborne Sp marker Ec marker insert remarks
RDB08041 pCtrlki-kanMX6 bacterial kanMX6 Ap MCS1-opal stop codon-kanMX6-MCS2 Plasmid vector to constract C-terminal truncated allele by the integration of opal stop codon into target gene using homologous recombination.
RDB08042 pCtFEM3ki-kanMX6 bacterial kanMX6 Ap MCS1-Flag-tev-3myc-opal stop codon-kanMX6-MCS2 Plasmid vector to construct C-terminally FEM3 (FLAG-tev-3xmyc) tagged allele of target gene by using homologous recombination.
RDB08045 pCtFLATAki-kanMX6 bacterial kanMX6 Ap MCS1-Flag-tev-protein A-opal stop codon-kanMX6-MCS2 Plasmid vector to construct C-terminally FLATA (FLAG-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08048 pFOX1-CFLATA replicative ScLEU2 (complement leu1-) Ap Ptef-MCS1-Flag-tev-protein A-opal stop codon-Ttef Plasmid vector to constract replicative expression clone constitutively expressing C-terminally FLATA tag (FLAG-tev-protein A) allele in leu1- strain of S. pombe.
RDB08051 pCtHATAki-kanMX6 bacterial kanMX6 Ap MCS1-HA-tev-protein A-opal stop codon-kanMX6-MCS2 Plasmid vector to constract C-terminally HATA (HA-tev-protein A) tagged allele of target gene by using homologous recombination.
RDB08052 pFOX1-CHATA replicative ScLEU2 (complement leu1-) Ap Ptef-MCS1-HA-tev-protein A-opal stop codon-Ttef Plasmid vector to constract replicative expression clone constitutively expressing C-terminally HATA tag (HA-tev-protein A) allele in leu1- strain of S. pombe.

S. pombe expression vectors (Gateway compatible)

Reference

  • Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.
  • Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M. ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombeNature Biotechnol. 2006 Jul; 24(7):841-7.
  • Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.
  • Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

 

pDUAL series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL series (Gateway-compatible)

Cat. num. Plasmid name Backbone Tag Tag
position
Promoter Multicopy marker Integration marker
RDB06130 pDUAL-HFF1c
pDUAL
His6-FLAG2
5′
nmt1
ura4+
leu1+
[sequence]
RDB06485 pDUAL-HFF21c
pDUAL
His6-FLAG2
5′
tub1
ura4+
leu1+
[sequence]
RDB06486 pDUAL-HFF31c
pDUAL
His6-FLAG2
5′
cam1
ura4+
leu1+
[sequence]
RDB06131 pDUAL-HFF41c pDUAL
His6-FLAG2
5′
nmt1*
ura4+
leu1+
[sequence]
RDB06487 pDUAL-HFF51c
pDUAL
His6-FLAG2
5′
tif51
ura4+
leu1+
[sequence]
RDB06488 pDUAL-HFF61c
pDUAL
His6-FLAG2
5′
ef1a-c
ura4+
leu1+
[sequence]
RDB06132 pDUAL-HFF81c
pDUAL
His6-FLAG2
5′
nmt1**
ura4+
leu1+
[sequence]
RDB06133 pDUAL-HFG1c
pDUAL
His6-FLAG-GFP(S65C)
5′
nmt1
ura4+
leu1+
[sequence]
RDB06493 pDUAL-HFG21c
pDUAL
His6-FLAG-GFP(S65C)
5′
tub1
ura4+
leu1+
[sequence]
RDB06494 pDUAL-HFG31c
pDUAL
His6-FLAG-GFP(S65C)
5′
cam1
ura4+
leu1+
[sequence]
RDB06134 pDUAL-HFG41c
pDUAL
His6-FLAG-GFP(S65C)
5′
nmt1*
ura4+
leu1+
[sequence]
RDB06495 pDUAL-HFG51c
pDUAL
His6-FLAG-GFP(S65C)
5′
tif51
ura4+
leu1+
[sequence]
RDB06496 pDUAL-HFG61c
pDUAL
His6-FLAG-GFP(S65C)
5′
ef1a-c
ura4+
leu1+
[sequence]
RDB06135 pDUAL-HFG81c
pDUAL
His6-FLAG-GFP(S65C)
5′
nmt1**
ura4+
leu1+
[sequence]
RDB06136 pDUAL-HFC1c
pDUAL
His6-FLAG-CFP
5′
nmt1
ura4+
leu1+
[sequence]
RDB06137 pDUAL-HFC41c
pDUAL
His6-FLAG-CFP
5′
nmt1*
ura4+
leu1+
[sequence]
RDB06138 pDUAL-HFC81c
pDUAL
His6-FLAG-CFP
5′
nmt1**
ura4+
leu1+
[sequence]
RDB06139 pDUAL-HFY1c
pDUAL
His6-FLAG-YFP
5′
nmt1
ura4+
leu1+
[sequence]
RDB06140 pDUAL-HFY41c
pDUAL
His6-FLAG-YFP
5′
nmt1*
ura4+
leu1+
[sequence]
RDB06141 pDUAL-HFY81c
pDUAL
His6-FLAG-YFP
5′
nmt1**
ura4+
leu1+
[sequence]
RDB06142 pDUAL-FFH1c
pDUAL
FLAG2-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06489 pDUAL-FFH21c
pDUAL
FLAG2-His6
3′
tub1
ura4+
leu1+
[sequence]
RDB06490 pDUAL-FFH31c
pDUAL
FLAG2-His6
3′
cam1
ura4+
leu1+
[sequence]
RDB06143 pDUAL-FFH41c
pDUAL
FLAG2-His6
3′
nmt1*
ura4+
leu1+
[sequence]
RDB06491 pDUAL-FFH51c
pDUAL
FLAG2-His6
3′
tif51
ura4+
leu1+
[sequence]
RDB06492 pDUAL-FFH61c
pDUAL
FLAG2-His6
3′
ef1a-c
ura4+
leu1+
[sequence]
RDB06144 pDUAL-FFH81c
pDUAL
FLAG2-His6
3′
nmt1**
ura4+
leu1+
[sequence]
RDB06145 pDUAL-GFH1c
pDUAL
GFP(S65C)-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06497 pDUAL-GFH21c
pDUAL
GFP(S65C)-FLAG-His6
3′
tub1
ura4+
leu1+
[sequence]
RDB06498 pDUAL-GFH31c
pDUAL
GFP(S65C)-FLAG-His6
3′
cam1
ura4+
leu1+
[sequence]
RDB06146 pDUAL-GFH41c
pDUAL
GFP(S65C)-FLAG-His6
3′
nmt1*
ura4+
leu1+
[sequence]
RDB06499 pDUAL-GFH51c
pDUAL
GFP(S65C)-FLAG-His6
3′
tif51
ura4+
leu1+
[sequence]
RDB06500 pDUAL-GFH61c
pDUAL
GFP(S65C)-FLAG-His6
3′
ef1a-c
ura4+
leu1+
[sequence]
RDB06147 pDUAL-GFH81c
pDUAL
GFP(S65C)-FLAG-His6
3′
nmt1**
ura4+
leu1+
[sequence]
RDB06148 pDUAL-CFH1c
pDUAL
CFP-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06149 pDUAL-CFH41c
pDUAL
CFP-FLAG-His6
3′
nmt1*
ura4+
leu1+
[sequence]
RDB06150 pDUAL-CFH81c
pDUAL
CFP-FLAG-His6
3′
nmt1**
ura4+
leu1+
[sequence]
RDB06151 pDUAL-YFH1c
pDUAL
YFP-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06152 pDUAL-YFH41c
pDUAL
YFP-FLAG-His6
3′
nmt1*
ura4+
leu1+
[sequence]
RDB06153 pDUAL-YFH81c
pDUAL
YFP-FLAG-His6
3′
nmt1**
ura4+
leu1+
[sequence]
RDB06481 pDUAL-FFH1c rfA
pDUAL
FLAG2-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06482 pDUAL-FFH1c rfC
pDUAL
FLAG2-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06483 pDUAL-YFH1c rfA
pDUAL
YFP-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06484 pDUAL-YFH1c rfC
pDUAL
YFP-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL2 series (Gateway-compatible)

Cat. num. Plasmid name Backbone Tag Tag
position
Promoter Multicopy marker Integration marker
RDB06154 pDUAL2-HFF1c
pDUAL2
His6-FLAG2
5′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06155 pDUAL2-HFF41c
pDUAL2
His6-FLAG2
5′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06156 pDUAL2-HFF81c
pDUAL2
His6-FLAG2
5′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06157 pDUAL2-HFG1c
pDUAL2
His6-FLAG-GFP(S65C)
5′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06158 pDUAL2-HFG41c
pDUAL2
His6-FLAG-GFP(S65C)
5′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06159 pDUAL2-HFG81c
pDUAL2
His6-FLAG-GFP(S65C)
5′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06160 pDUAL2-HFC1c
pDUAL2
His6-FLAG-CFP
5′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06161 pDUAL2-HFC41c
pDUAL2
His6-FLAG-CFP
5′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06162 pDUAL2-HFC81c
pDUAL2
His6-FLAG-CFP
5′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06163 pDUAL2-HFY1c
pDUAL2
His6-FLAG-YFP
5′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06164 pDUAL2-HFY41c
pDUAL2
His6-FLAG-YFP
5′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06165 pDUAL2-HFY81c
pDUAL2
His6-FLAG-YFP
5′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06166 pDUAL2-FFH1c
pDUAL2
FLAG2-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06167 pDUAL2-FFH41c
pDUAL2
FLAG2-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06168 pDUAL2-FFH81c
pDUAL2
FLAG2-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06169 pDUAL2-GFH1c
pDUAL2
GFP(S65C)-FLAG-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06170 pDUAL2-GFH41c
pDUAL2
GFP(S65C)-FLAG-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06171 pDUAL2-GFH81c
pDUAL2
GFP(S65C)-FLAG-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06172 pDUAL2-CFH1c
pDUAL2
CFP-FLAG-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06173 pDUAL2-CFH41c
pDUAL2
CFP-FLAG-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06174 pDUAL2-CFH81c
pDUAL2
CFP-FLAG-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06175 pDUAL2-YFH1c
pDUAL2
YFP-FLAG-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06503 pDUAL2-YFH21c
pDUAL2
YFP-FLAG-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06176 pDUAL2-YFH41c
pDUAL2
YFP-FLAG-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06177 pDUAL2-YFH81c
pDUAL2
YFP-FLAG-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06501 pDUAL2-BFH41c
pDUAL2
BFP-FLAG-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06502 pDUAL2-BFH81c
pDUAL2
BFP-FLAG-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pREP series (Gateway-compatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Selection marker
RDB06519 pREP1-ccdB2
pREP1
nmt1
LEU2
[sequence]
RDB06520 pREP41-ccdB2
pREP41
nmt1*
LEU2
[sequence]
RDB06521 pREP81-ccdB2
pREP81
nmt1**
LEU2
[sequence]
RDB06522 pHFF1-ccdB2
pREP1
His6-FLAG2
5′
nmt1
LEU2
[sequence]
RDB06523 pHFF41-ccdB2
pREP41
His6-FLAG2
5′
nmt1*
LEU2
[sequence]
RDB06524 pHFF81-ccdB2
pREP81
His6-FLAG2
5′
nmt1**
LEU2
[sequence]
RDB06525 pHFG1-ccdB2
pREP1
His6-FLAG-GFP(S65C)
5′
nmt1
LEU2
[sequence]
RDB06526 pHFG41-ccdB2
pREP41
His6-FLAG-GFP(S65C)
5′
nmt1*
LEU2
[sequence]
RDB06527 pHFG81-ccdB2
pREP81
His6-FLAG-GFP(S65C)
5′
nmt1**
LEU2
[sequence]
RDB06506 pFFH1-ccdB2
pREP1
FLAG2-His6
3′
nmt1
LEU2
[sequence]
RDB06507 pFFH41-ccdB2
pREP41
FLAG2-His6
3′
nmt1*
LEU2
[sequence]
RDB06508 pFFH81-ccdB2
pREP81
FLAG2-His6
3′
nmt1**
LEU2
[sequence]
RDB06509 pGFH1-ccdB2
pREP1
GFP(S65C)-FLAG-His6
3′
nmt1
LEU2
[sequence]
RDB06510 pGFH41-ccdB2
pREP41
GFP(S65C)-FLAG-His6
3′
nmt1*
LEU2
[sequence]
RDB06511 pGFH81-ccdB2
pREP81
GFP(S65C)-FLAG-His6
3′
nmt1**
LEU2
[sequence]
RDB06513 pHFY1-ccdB2
pREP1
His6-FLAG-YFP
5′
nmt1
LEU2
[sequence]
RDB06512 pHFC1-ccdB2
pREP1
His6-FLAG-CFP
5′
nmt1
LEU2
[sequence]
RDB06515 pYFH1-ccdB2
pREP1
YFP-FLAG-His6
3′
nmt1
LEU2
[sequence]
RDB06505 pYFH1-ccdB2 rfA
pREP1
YFP-FLAG-His6
3′
nmt1
LEU2
[sequence]
RDB06504 pYFH1-ccdB2 rfC
pREP1
YFP-FLAG-His6
3′
nmt1
LEU2
[sequence]
RDB06514 pCFH1-ccdB2
pREP1
CFP-FLAG-His6
3′
nmt1
LEU2
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

Gateway-compatible vectors for chromosomal integration

Cat. num.
Plasmid name
Backbone
Promoter
Tag
Tag
position
Multicopy marker
Targeted locus
(negative) selection
Positive selection marker
RDB06462 pLYS1U-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06463 pLYS1U-CFH21c
pUC119
tub1
CFP-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06464 pLYS1U-CFH61c
pUC119
ef1a-c
CFP-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06465 pLYS1U-FFH1c
pUC119
nmt1
FLAG2-His6
3′
lys1+
ura4+
[sequence]
RDB06466 pLYS1U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06467 pLYS1U-GFH21c
pUC119
tub1
GFP(S65C)-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06468 pLYS1U-GFH61c
pUC119
ef1a-c
GFP(S65C)-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06477 pLYS1U-YFH1c
pUC119
nmt1
YFP-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06478 pLYS1U-YFH21c
pUC119
tub1
YFP-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06479 pLYS1U-YFH61c
pUC119
ef1a-c
YFP-FLAG-His6
3′
lys1+
ura4+
[sequence]
RDB06469 pLYS1U-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5′
lys1+
ura4+
[sequence]
RDB06470 pLYS1U-HFC21c
pUC119
tub1
His6-FLAG-CFP
5′
lys1+
ura4+
[sequence]
RDB06471 pLYS1U-HFC61c
pUC119
ef1a-c
His6-FLAG-CFP
5′
lys1+
ura4+
[sequence]
RDB06472 pLYS1U-HFF1c
pUC119
nmt1
His6-FLAG2
5′
lys1+
ura4+
[sequence]
RDB06473 pLYS1U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5′
lys1+
ura4+
[sequence]
RDB06474 pLYS1U-HFY1c
pUC119
nmt1
His6-FLAG-YFP
5′
lys1+
ura4+
[sequence]
RDB06475 pLYS1U-HFY21c
pUC119
tub1
His6-FLAG-YFP
5′
lys1+
ura4+
[sequence]
RDB06476 pLYS1U-HFY61c
pUC119
ef1a-c
His6-FLAG-YFP
5′
lys1+
ura4+
[sequence]
RDB06458 pLYS1K-FFH1c
pUC119
nmt1
FLAG2-His6
3′
lys1+
kanr
[sequence]
RDB06459 pLYS1K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3′
lys1+
kanr
[sequence]
RDB06460 pLYS1K-HFF1c
pUC119
nmt1
His6-FLAG2
5′
lys1+
kanr
[sequence]
RDB06461 pLYS1K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5′
lys1+
kanr
[sequence]
RDB06517 pHIS3U-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3′
his3+
ura4+
[sequence]
RDB06455 pHIS3U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3′
his3+
ura4+
[sequence]
RDB06518 pHIS3U-YFH1c
pUC119
nmt1
YFP-FLAG-His6
3′
his3+
ura4+
[sequence]
RDB06456 pHIS3U-HFF1c
pUC119
nmt1
His6-FLAG2
5′
his3+
ura4+
[sequence]
RDB06516 pHIS3U-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5′
his3+
ura4+
[sequence]
RDB06457 pHIS3U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5′
his3+
ura4+
[sequence]
RDB06445 pHIS3K-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3′
his3+
kanr
[sequence]
RDB06446 pHIS3K-CFH21c
pUC119
tub1
CFP-FLAG-His6
3′
his3+
kanr
[sequence]
RDB06447 pHIS3K-CFH61c
pUC119
ef1a-c
CFP-FLAG-His6
3′
his3+
kanr
[sequence]
RDB06448 pHIS3K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3′
his3+
kanr
[sequence]
RDB06449 pHIS3K-GFH21c
pUC119
tub1
GFP(S65C)-FLAG-His6
3′
his3+
kanr
[sequence]
RDB06450 pHIS3K-GFH61c
pUC119
ef1a-c
GFP(S65C)-FLAG-His6
3′
his3+
kanr
[sequence]
RDB06451 pHIS3K-HFC1c
pUC119
nmt1
His6-FLAG-CFP
5′
his3+
kanr
[sequence]
RDB06452 pHIS3K-HFC21c
pUC119
tub1
His6-FLAG-CFP
5′
his3+
kanr
[sequence]
RDB06453 pHIS3K-HFF1c
pUC119
nmt1
His6-FLAG2
5′
his3+
kanr
[sequence]
RDB06454 pHIS3K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5′
his3+
kanr
[sequence]
RDB06441 pARG1U-FFH1c
pUC119
nmt1
FLAG2-His6
3′
arg1+
ura4+
[sequence]
RDB06442 pARG1U-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3′
arg1+
ura4+
[sequence]
RDB06443 pARG1U-HFF1c
pUC119
nmt1
His6-FLAG2
5′
arg1+
ura4+
[sequence]
RDB06444 pARG1U-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5′
arg1+
ura4+
[sequence]
RDB06437 pARG1K-FFH1c
pUC119
nmt1
FLAG2-His6
3′
arg1+
kanr
[sequence]
RDB06438 pARG1K-GFH1c
pUC119
nmt1
GFP(S65C)-FLAG-His6
3′
arg1+
kanr
[sequence]
RDB06439 pARG1K-HFF1c
pUC119
nmt1
His6-FLAG2
5′
arg1+
kanr
[sequence]
RDB06440 pARG1K-HFG1c
pUC119
nmt1
His6-FLAG-GFP(S65C)
5′
arg1+
kanr
[sequence]
RDB06480 pARG1KF-CFH1c
pUC119
nmt1
CFP-FLAG-His6
3′
ura4+
arg1+
kanr
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

S. pombe expression vectors

Reference

  • Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast. 2004 Nov;21(15):1289-305.
  • Matsuyama A, Shirai A, Yoshida M. A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast 2008 May;25(5):371-6.
  • Matsuyama A, Shirai A, Yoshida M. A novel series of vectors for chromosomal integration in fission yeast. Biochem Biophys Res Commun. 2008 Sep 19;374(2): 315-9.

pDUAL series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL series (Gateway-incompatible)

Cat. num. Plasmid name Backbone Tag Tag
position
Promoter Multicopy marker Integration marker
RDB06178 pDUAL
(pUC119)
ura4+
leu1+
[sequence]
RDB06528 pDUAL-S
(pUC119)
ura4+
leu1+
[sequence]
RDB06529 pDUAL-Pef1a
pDUAL
ef1a-c
ura4+
leu1+
[sequence]
RDB06530 pDUAL-Ptub1
pDUAL
tub1
ura4+
leu1+
[sequence]
RDB06179 pDUAL-HFF1
pDUAL
His6-FLAG2
5′
nmt1
ura4+
leu1+
[sequence]
RDB06531 pDUAL-HFF21
pDUAL
His6-FLAG2
5′
tub1
ura4+
leu1+
[sequence]
RDB06532 pDUAL-HFF31
pDUAL
His6-FLAG2
5′
cam1
ura4+
leu1+
[sequence]
RDB06180 pDUAL-HFF41
pDUAL
His6-FLAG2
5′
nmt1*
ura4+
leu1+
[sequence]
RDB06533 pDUAL-HFF51
pDUAL
His6-FLAG2
5′
tif51
ura4+
leu1+
[sequence]
RDB06534 pDUAL-HFF61
pDUAL
His6-FLAG2
5′
ef1a-c
ura4+
leu1+
[sequence]
RDB06181 pDUAL-HFF81
pDUAL
His6-FLAG2
5′
nmt1**
ura4+
leu1+
[sequence]
RDB06182 pDUAL-HFG1
pDUAL
His6-FLAG-GFP(S65C)
5′
nmt1
ura4+
leu1+
[sequence]
RDB06539 pDUAL-HFG21
pDUAL
His6-FLAG-GFP(S65C)
5′
tub1
ura4+
leu1+
[sequence]
RDB06540 pDUAL-HFG31
pDUAL
His6-FLAG-GFP(S65C)
5′
cam1
ura4+
leu1+
[sequence]
RDB06183 pDUAL-HFG41
pDUAL
His6-FLAG-GFP(S65C)
5′
nmt1*
ura4+
leu1+
[sequence]
RDB06541 pDUAL-HFG51
pDUAL
His6-FLAG-GFP(S65C)
5′
tif51
ura4+
leu1+
[sequence]
RDB06542 pDUAL-HFG61
pDUAL
His6-FLAG-GFP(S65C)
5′
ef1a-c
ura4+
leu1+
[sequence]
RDB06184 pDUAL-HFG81
pDUAL
His6-FLAG-GFP(S65C)
5′
nmt1**
ura4+
leu1+
[sequence]
RDB06185 pDUAL-FFH1
pDUAL
FLAG2-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06535 pDUAL-FFH21
pDUAL
FLAG2-His6
3′
tub1
ura4+
leu1+
[sequence]
RDB06536 pDUAL-FFH31
pDUAL
FLAG2-His6
3′
cam1
ura4+
leu1+
[sequence]
RDB06186 pDUAL-FFH41
pDUAL
FLAG2-His6
3′
nmt1*
ura4+
leu1+
[sequence]
RDB06537 pDUAL-FFH51
pDUAL
FLAG2-His6
3′
tif51
ura4+
leu1+
[sequence]
RDB06538 pDUAL-FFH61
pDUAL
FLAG2-His6
3′
ef1a-c
ura4+
leu1+
[sequence]
RDB06187 pDUAL-FFH81
pDUAL
FLAG2-His6
3′
nmt1**
ura4+
leu1+
[sequence]
RDB06188 pDUAL-GFH1
pDUAL
GFP(S65C)-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]
RDB06543 pDUAL-GFH21
pDUAL
GFP(S65C)-FLAG-His6
3′
tub1
ura4+
leu1+
[sequence]
RDB06544 pDUAL-GFH31
pDUAL
GFP(S65C)-FLAG-His6
3′
cam1
ura4+
leu1+
[sequence]
RDB06189 pDUAL-GFH41
pDUAL
GFP(S65C)-FLAG-His6
3′
nmt1*
ura4+
leu1+
[sequence]
RDB06545 pDUAL-GFH51
pDUAL
GFP(S65C)-FLAG-His6
3′
tif51
ura4+
leu1+
[sequence]
RDB06546 pDUAL-GFH61
pDUAL
GFP(S65C)-FLAG-His6
3′
ef1a-c
ura4+
leu1+
[sequence]
RDB06190 pDUAL-GFH81
pDUAL
GFP(S65C)-FLAG-His6
3′
nmt1**
ura4+
leu1+
[sequence]
RDB06191 pDUAL-YFH1
pDUAL
YFP-FLAG-His6
3′
nmt1
ura4+
leu1+
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pDUAL2 series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pDUAL2 series (Gateway-incompatible)

Cat. num. Plasmid name Backbone Tag Tag
position
Promoter Multicopy marker Integration marker
RDB06192 pDUAL2
(pUC119)
ura4+
ura4+leu1
[sequence]
RDB06193 pDUAL2-HFF1
pDUAL2
His6-FLAG2
5′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06547 pDUAL2-HFF21
pDUAL2
His6-FLAG2
5′
tub1
ura4+
ura4+leu1
[sequence]
RDB06194 pDUAL2-HFF41
pDUAL2
His6-FLAG2
5′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06549 pDUAL2-HFF61
pDUAL2
His6-FLAG2
5′
ef1a-c
ura4+
ura4+leu1
[sequence]
RDB06195 pDUAL2-HFF81
pDUAL2
His6-FLAG2
5′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06196 pDUAL2-HFG1
pDUAL2
His6-FLAG-GFP(S65C)
5′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06197 pDUAL2-HFG41
pDUAL2
His6-FLAG-GFP(S65C)
5′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06198 pDUAL2-HFG81
pDUAL2
His6-FLAG-GFP(S65C)
5′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06199 pDUAL2-FFH1
pDUAL2
FLAG2-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06548 pDUAL2-FFH21
pDUAL2
FLAG2-His6
3′
tub1
ura4+
ura4+leu1
[sequence]
RDB06200 pDUAL2-FFH41
pDUAL2
FLAG2-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06550 pDUAL2-FFH61
pDUAL2
FLAG2-His6
3′
ef1a-c
ura4+
ura4+leu1
[sequence]
RDB06201 pDUAL2-FFH81
pDUAL2
FLAG2-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06202 pDUAL2-GFH1
pDUAL2
GFP(S65C)-FLAG-His6
3′
nmt1
ura4+
ura4+leu1
[sequence]
RDB06203 pDUAL2-GFH41
pDUAL2
GFP(S65C)-FLAG-His6
3′
nmt1*
ura4+
ura4+leu1
[sequence]
RDB06204 pDUAL2-GFH81
pDUAL2
GFP(S65C)-FLAG-His6
3′
nmt1**
ura4+
ura4+leu1
[sequence]
RDB06551 pDUAL2-CFH61
pDUAL2
CFP-FLAG-His6
3′
ef1a-c
ura4+
ura4+leu1
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

pREP series

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

pREP series (Gateway-incompatible)

Cat. num.
Plasmid name
Backbone
Tag
Tag
position
Promoter
Selection marker
RDB06580 pHFF1
pREP1
His6-FLAG2
5′
nmt1
LEU2
[sequence]
RDB06581 pHFF41
pREP41
His6-FLAG2
5′
nmt1*
LEU2
[sequence]
RDB06582 pHFF81
pREP81
His6-FLAG2
5′
nmt1**
LEU2
[sequence]
RDB06592 pHFF148
(pREP)
His6-FLAG2
5′
ADH1
LEU2
[sequence]
RDB06583 pHFG1
pREP1
His6-FLAG-GFP(S65C)
5′
nmt1
LEU2
[sequence]
RDB06584 pHFG41
pREP41
His6-FLAG-GFP(S65C)
5′
nmt1*
LEU2
[sequence]
RDB06585 pHFG81
pREP81
His6-FLAG-GFP(S65C)
5′
nmt1**
LEU2
[sequence]
RDB06593 pHFG148
(pREP)
His6-FLAG-GFP(S65C)
5′
ADH1
LEU2
[sequence]
RDB06586 pFFH1
pREP1
FLAG2-His6
3′
nmt1
LEU2
[sequence]
RDB06587 pFFH41
pREP41
FLAG2-His6
3′
nmt1*
LEU2
[sequence]
RDB06588 pFFH81
pREP81
FLAG2-His6
3′
nmt1**
LEU2
[sequence]
RDB06594 pFFH148
(pREP)
FLAG2-His6
3′
ADH1
LEU2
[sequence]
RDB06589 pGFH1
pREP1
GFP(S65C)-FLAG-His6
3′
nmt1
LEU2
[sequence]
RDB06590 pGFH41
pREP41
GFP(S65C)-FLAG-His6
3′
nmt1*
LEU2
[sequence]
RDB06591 pGFH81
pREP81
GFP(S65C)-FLAG-His6
3′
nmt1**
LEU2
[sequence]
RDB06595 pGFH148
(pREP)
GFP(S65C)-FLAG-His6
3′
ADH1
LEU2
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

Integration vectors

Vector sequence is abailable at SPD, Chemical Genetics Laboratory.

Gateway-incompatible vectors for chromosomal integration

Cat. num.
Plasmid name
Backbone
Promoter
Multicopy marker
Targeted locus
Positive selection marker
RDB06552 pLYS1U
pUC119
lys1+
ura4+
[sequence]
RDB06567 pLYS1U-Pnmt1
pUC119
nmt1
lys1+
ura4+
[sequence]
RDB06566 pLYS1U-Ptub1
pUC119
tub1
lys1+
ura4+
[sequence]
RDB06553 pLYS1K
pUC119
lys1+
kanr
[sequence]
RDB06554 pLYS1H
pUC119
lys1+
hygr
[sequence]
RDB06555 pLYS1B
pUC119
lys1+
blar
[sequence]
RDB06560 pHIS3U
pUC119
his3+
ura4+
[sequence]
RDB06565 pHIS3U-Pnmt1
pUC119
nmt1
his3+
ura4+
[sequence]
RDB06561 pHIS3K
pUC119
his3+
kanr
[sequence]
RDB06564 pHIS3K-Ptub1
pUC119
tub1
his3+
kanr
[sequence]
RDB06562 pHIS3H
pUC119
his3+
hygr
[sequence]
RDB06563 pHIS3B
pUC119
his3+
blar
[sequence]
RDB06556 pARG1U
pUC119
arg1+
ura4+
[sequence]
RDB06557 pARG1K
pUC119
arg1+
kanr
[sequence]
RDB06558 pARG1H
pUC119
arg1+
hygr
[sequence]
RDB06559 pARG1B
pUC119
arg1+
blar
[sequence]
RDB06573 pLYS1UF
pUC119
ura4+
lys1+
ura4+
[sequence]
RDB06572 pLYS1KF
pUC119
ura4+
lys1+
kanr
[sequence]
RDB06574 pLYS1HF
pUC119
ura4+
lys1+
hygr
[sequence]
RDB06575 pLYS1BF
pUC119
ura4+
lys1+
blar
[sequence]
RDB06569 pHIS3UF
pUC119
ura4+
his3+
ura4+
[sequence]
RDB06568 pHIS3KF
pUC119
ura4+
his3+
kanr
[sequence]
RDB06570 pHIS3HF
pUC119
ura4+
his3+
hygr
[sequence]
RDB06571 pHIS3BF
pUC119
ura4+
his3+
blar
[sequence]
RDB06577 pARG1UF
pUC119
ura4+
arg1+
ura4+
[sequence]
RDB06576 pARG1KF
pUC119
ura4+
arg1+
kanr
[sequence]
RDB06578 pARG1HF
pUC119
ura4+
arg1+
hygr
[sequence]
RDB06579 pARG1BF
pUC119
ura4+
arg1+
blar
[sequence]

* Medium strength nmt1 promoter, ** Low strength nmt1 promoter

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(GRP0027e 2011.09.30 T.M.)

2019.02.02