Expression regulation


Conditional Gene Expression

Cre-loxP system

Vector backbone

RDB15114 pBSIISK-hFLEX
Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch.
RDB04397 pUSwS
A cassette plasmid for inserting DNA between two loxP S sequences in the same orientation
RDB04396 pUVwV
A cassette plasmid for inserting DNA between two loxP V sequences in the same orientation
RDB03446 pUAd5LCALNL(W1975C23R)
Shuttle vector to generate recombinant adenovirus
RDB01862 pCALNL5
A cassette to introduce cDNA into mammalian chromosome
RDB01679 pCALwL
A cassette plasmid to insert cDNA into downstream CAG promoter
RDB01677 pULwL
A cassette plasmid to insert stuffer DNA between two loxP site
RDB01674 pCALNLw
A cassette to introduce cDNA into mammalian chromosome

 

Cre expression

RDB16797 pAcGFP-NCre
Mammalian expression vector encoding Cre recombinase tagged with AcGFP1.
RDB15053 pK073.TRE-Dre-WPRE (Supernova)
Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase.
RDB15050 pK170.AAV-TRE-Cre-WPRE (Supernova)
Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.
RDB15043 pK031.TRE-Cre (Supernova)
Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.
RDB14533 pCS2-OlCre
Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka).
RDB12117 pPyCAG-CreGR-IB
Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. Blasticidin S resistance.
RDB09596 pPyCAG-Cre-IRESpuropA
Expression vector of Cre recombinase. Puromycin resistance.
RDB08998 pCAGGS-Cre
Expression clone of Cre recombinase.
RDB07106 pPyCAG-CreGR-ID
Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance.
RDB06073 pKM2C
Reporter construct with Cre recombinase gene
RDB01748 AxCANCre
Recombinant adenovirus expressing recombinase Cre with nuclear localization signal
RDB01675 pxCANCre
A plasmid expressing the recombinase Cre tagged with a nls

 

Arranged vector

RDB16747 pCALNL5:mCherry
Cre recombinase inducible expression cassette of mCherry.
RDB06232 pxCALNLEYFP
Expression vector of EYFP
RDB03347 AxCALNLEGFP (forward)
Recombinant adenovirus harboring enhanced green fluorescent protein cDNA
RDB03262 pAxCALNLEYFP (reverse)
Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA
RDB03261 pAxCALNLEYFP (forward)
Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA.
RDB03260 pAxCALNLEGFP (reverse)
Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA
RDB03259 pAxCALNLEGFP (forward)
Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA.
RDB03252 pAxCALNLBFP (reverse)
Shuttle vector for rAd harboring blue fluorescent protein cDNA
RDB03251 pAxCALNLBFP (forward)
Shuttle vector for rAd harboring blue fluorescent protein cDNA.
RDB01750 AxCALNLNZ
Recombinant adenovirus expressing E. coli lacZ
RDB01680 pCALNLZ
A control plasmid to introduce LacZ gene into mammalian chromosome

Plasmid clone pxCALNLEYFP (RDB06232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB01675), which expresses Cre recombinase.

Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).


 

FLP-FRT system

FLP expression

RDB16011 pT7-3xFLAG-NLS-Flpe-polyA plasmid
FLAG-NLS-Flpe-myc expressing plasmid.
RDB15046 pK036.TRE-Flpe-WPRE (Supernova)
Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase.
RDB08124 pAxEFhFLPeit2
Shuttle vector to generate recombinant adenovirus expressing humanized FLPe
RDB08123 AxEFhFLPe
Recombinant adenovirus expressing humanized FLPe
RDB07083 pxCAhFLPe
Expression vector of site-specific recombinase, FLP

 

Inducible Gene Expression

Tetracycline inducible system

Vector backbone

RDB09232 pBlKS hCMV*-1 b-globinpA
Expression vector of tet-inducible CMV promoter b-globin poly(A) casette.
RDB06012 pAdex TRE-SwaI
Shuttle vector to generate rAd with Tet-responsive element (TRE) promoter

 

Tet regulatory protein expression

RDB18685 pAAV2-SYN-tTAad-BGHpA
AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.
RDB15056 pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)
Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed.
RDB15055 pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)
Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter.
RDB15047 pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter.
RDB15045 pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.
RDB13212 pAAV-SP-FtTA
AAV vector of FLAG-tTA under the control of Substance P promoter
RDB13211 pAAV-Enk-FtTA
AAV vector of FLAG-tTA under the control of Enkephalin promoter
RDB12109 pBRPyCAG-tTAFF-IN
RDB02351 Ax1 CM-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01733 Ax1 CM-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01732 Ax1-CA-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01731 Ax1-Tet NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01730 Ax1-RxNtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01711 pAx1-CM-NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01710 pAx1-CA-NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01709 pAx1-Tet NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01708 pAx1-RxNtTA
Recombinant adenoviral vector expressing NtTA (Tet-transactivator)
RDB01684 pCM-NtTA
CM-NtTA (Tet-transactivator driven by CM promoter)
RDB01683 pTet NtTA
Tet-NtTA (Tet-transactivator driven by tet promoter)
RDB01583 pAxCMtTA
Shuttle vector to generate rAd expressing E. coli Tet-inducible tTA gene

 

Arranged vector

RDB01584 pAxTetZ
Shuttle vector to generate rAd expressing tet-inducible lacZ
RDB01574 AxTetZ
Recombinant adenovirus expressing Tet-inducible lacZ gene

 


Inducible protein degradation

Auxin Inducible Degron (AID) System

Using the AID System, you can degrade proteins fused with a degron (a small domain that can induce protein degradation) in a very short period of time.
 

(GRP0073e 2004.08.22 T.M.)

2022.08.28



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