Conditional Gene Expression
Cre-loxP system
Vector backbone
| RDB15114 | pBSIISK-hFLEX Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch. |
| RDB04397 | pUSwS A cassette plasmid for inserting DNA between two loxP S sequences in the same orientation |
| RDB04396 | pUVwV A cassette plasmid for inserting DNA between two loxP V sequences in the same orientation |
| RDB03446 | pUAd5LCALNL(W1975C23R) Shuttle vector to generate recombinant adenovirus |
| RDB01862 | pCALNL5 A cassette to introduce cDNA into mammalian chromosome |
| RDB01679 | pCALwL A cassette plasmid to insert cDNA into downstream CAG promoter |
| RDB01677 | pULwL A cassette plasmid to insert stuffer DNA between two loxP site |
| RDB01674 | pCALNLw A cassette to introduce cDNA into mammalian chromosome |
Cre expression
| RDB16797 | pAcGFP-NCre Mammalian expression vector encoding Cre recombinase tagged with AcGFP1. |
| RDB15053 | pK073.TRE-Dre-WPRE (Supernova) Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase. |
| RDB15050 | pK170.AAV-TRE-Cre-WPRE (Supernova) Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. |
| RDB15043 | pK031.TRE-Cre (Supernova) Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. |
| RDB14533 | pCS2-OlCre Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka). |
| RDB12117 | pPyCAG-CreGR-IB Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. Blasticidin S resistance. |
| RDB09596 | pPyCAG-Cre-IRESpuropA Expression vector of Cre recombinase. Puromycin resistance. |
| RDB08998 | pCAGGS-Cre Expression clone of Cre recombinase. |
| RDB07106 | pPyCAG-CreGR-ID Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance. |
| RDB06073 | pKM2C Reporter construct with Cre recombinase gene |
| RDB01748 | AxCANCre Recombinant adenovirus expressing recombinase Cre with nuclear localization signal |
| RDB01675 | pxCANCre A plasmid expressing the recombinase Cre tagged with a nls |
Arranged vector
| RDB16747 | pCALNL5:mCherry Cre recombinase inducible expression cassette of mCherry. |
| RDB06232 | pxCALNLEYFP Expression vector of EYFP |
| RDB03347 | AxCALNLEGFP (forward) Recombinant adenovirus harboring enhanced green fluorescent protein cDNA |
| RDB03262 | pAxCALNLEYFP (reverse) Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA |
| RDB03261 | pAxCALNLEYFP (forward) Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA. |
| RDB03260 | pAxCALNLEGFP (reverse) Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA |
| RDB03259 | pAxCALNLEGFP (forward) Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA. |
| RDB03252 | pAxCALNLBFP (reverse) Shuttle vector for rAd harboring blue fluorescent protein cDNA |
| RDB03251 | pAxCALNLBFP (forward) Shuttle vector for rAd harboring blue fluorescent protein cDNA. |
| RDB01750 | AxCALNLNZ Recombinant adenovirus expressing E. coli lacZ |
| RDB01680 | pCALNLZ A control plasmid to introduce LacZ gene into mammalian chromosome |
Plasmid clone pxCALNLEYFP (RDB06232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB01675), which expresses Cre recombinase.
Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).
FLP-FRT system
FLP expression
| RDB16011 | pT7-3xFLAG-NLS-Flpe-polyA plasmid FLAG-NLS-Flpe-myc expressing plasmid. |
| RDB15046 | pK036.TRE-Flpe-WPRE (Supernova) Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase. |
| RDB08124 | pAxEFhFLPeit2 Shuttle vector to generate recombinant adenovirus expressing humanized FLPe |
| RDB08123 | AxEFhFLPe Recombinant adenovirus expressing humanized FLPe |
| RDB07083 | pxCAhFLPe Expression vector of site-specific recombinase, FLP |
Inducible Gene Expression
Tetracycline inducible system
Vector backbone
| RDB09232 | pBlKS hCMV*-1 b-globinpA Expression vector of tet-inducible CMV promoter b-globin poly(A) casette. |
| RDB06012 | pAdex TRE-SwaI Shuttle vector to generate rAd with Tet-responsive element (TRE) promoter |
Tet regulatory protein expression
| RDB18685 | pAAV2-SYN-tTAad-BGHpA AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I. |
| RDB15056 | pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova) Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed. |
| RDB15055 | pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova) Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter. |
| RDB15047 | pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter. |
| RDB15045 | pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter. |
| RDB13212 | pAAV-SP-FtTA AAV vector of FLAG-tTA under the control of Substance P promoter |
| RDB13211 | pAAV-Enk-FtTA AAV vector of FLAG-tTA under the control of Enkephalin promoter |
| RDB12109 | pBRPyCAG-tTAFF-IN |
| RDB02351 | Ax1 CM-NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01733 | Ax1 CM-NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01732 | Ax1-CA-NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01731 | Ax1-Tet NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01730 | Ax1-RxNtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01711 | pAx1-CM-NtTA Shuttle vector to generate rAd expressing NtTA |
| RDB01710 | pAx1-CA-NtTA Shuttle vector to generate rAd expressing NtTA |
| RDB01709 | pAx1-Tet NtTA Shuttle vector to generate rAd expressing NtTA |
| RDB01708 | pAx1-RxNtTA Recombinant adenoviral vector expressing NtTA (Tet-transactivator) |
| RDB01684 | pCM-NtTA CM-NtTA (Tet-transactivator driven by CM promoter) |
| RDB01683 | pTet NtTA Tet-NtTA (Tet-transactivator driven by tet promoter) |
| RDB01583 | pAxCMtTA Shuttle vector to generate rAd expressing E. coli Tet-inducible tTA gene |
Arranged vector
| RDB01584 | pAxTetZ Shuttle vector to generate rAd expressing tet-inducible lacZ |
| RDB01574 | AxTetZ Recombinant adenovirus expressing Tet-inducible lacZ gene |
Inducible protein degradation
Auxin Inducible Degron (AID) System
Using the AID System, you can degrade proteins fused with a degron (a small domain that can induce protein degradation) in a very short period of time.
(GRP0073e 2004.08.22 T.M.)
2022.08.28
