Cre-loxP and FLP-FRT System
Plasmid clone pxCALNLEYFP (RDB06232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB01675), which expresses Cre recombinase.
Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).
- Cre-loxP
Catalog no. Name of resource RDB01748 AxCANCre
Recombinant adenovirus expressing recombinase Cre with nuclear localization signalRDB06073 pKM2C
Reporter construct with Cre recombinase geneRDB01675 pxCANCre
A plasmid expressing the recombinase Cre tagged with a nlsRDB08998 pCAGGS-Cre
Expression clone of Cre recombinase.RDB09596 pPyCAG-Cre-IP
Expression clone of Cre recombinase. Puromycin resistance.RDB12117 pPyCAG-CreGR-IB
Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. BSD resistance.RDB07106 pPyCAG-CreGR-ID
Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance.RDB03347 AxCALNLEGFP (forward)
Recombinant adenovirus harboring enhanced green fluorescent protein cDNARDB03251 pAxCALNLBFP (forward)
Shuttle vector for rAd harboring blue fluorescent protein cDNARDB03252 pAxCALNLBFP (reverse)
Shuttle vector for rAd harboring blue fluorescent protein cDNARDB03259 pAxCALNLEGFP (forward)
Shuttle vector for rAd harboring enhanced green fluorescent protein cDNARDB03260 pAxCALNLEGFP (reverse)
Shuttle vector for rAd harboring enhanced green fluorescent protein cDNARDB03261 pAxCALNLEYFP (forward)
Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNARDB03262 pAxCALNLEYFP (reverse)
Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNARDB06232 pxCALNLEYFP
Expression vector of EYFPRDB01750 AxCALNLNZ
Recombinant adenovirus expressing E. coli lacZRDB01862 pCALNL5
A cassette to introduce cDNA into mammalian chromosomeRDB01674 pCALNLw
A cassette to introduce cDNA into mammalian chromosomeRDB01680 pCALNLZ
A control plasmid to introduce LacZ gene into mammalian chromosomeRDB01679 pCALwL
A cassette plasmid to insert cDNA into downstream CAG promoterRDB03448 pUAd5LCALNL(W1975C1F)
Shuttle vector to generate recombinant adenovirusRDB03446 pUAd5LCALNL(W1975C23R)
Shuttle vector to generate recombinant adenovirusRDB01677 pULwL
A cassette plasmid to insert stuffer DNA between two loxP siteRDB04397 pUSwS
A cassette plasmid for inserting DNA between two loxP S sequences in the same orientationRDB04396 pUVwV
A cassette plasmid for inserting DNA between two loxP V sequences in the same orientationRDB15114 pBSIISK-hFLEX
Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch.RDB14533 pCS2-OlCre
Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka)RDB16747 pCALNL5:mCherry
Cre recombinase inducible expression cassette of mCherry.RDB16797 pAcGFP-NCre
Mammalian expression vector encoding Cre recombinase tagged with AcGFP1. - FLP-FRT
Catalog no. Name of resource RDB08123 AxEFhFLPe
Recombinant adenovirus expressing humanized FLPeRDB08124 pAxEFhFLPeit2
Shuttle vector to generate recombinant adenovirus expressing humanized FLPeRDB07083 pxCAhFLPe
Expression vector of site-specific recombinase, FLP
Tet Inducible System
| Catalog no. | Name of resource |
|---|---|
| RDB13211 | pAAV-Enk-FtTA AAV vector of tTA under the control of Enkephalin promoter |
| RDB13212 | pAAV-SP-FtTA AAV vector of tTA under the control of Substance P promoter |
| RDB01683 | pTet NtTA Tet-transactivator driven by tet-operator promoter |
| RDB01684 | pCM-NtTA Tet-transactivator driven by CM promoter |
| RDB09232 | pBlKS hCMV*-1 b-globinpA Expression vector of tet-inducible CMV promoter b-globin poly(A) casette. |
| RDB12109 | pBRPyCAG-tTAFF-IN Expression vector of DNA binding domain from tTA and two transcription activation modules from VP16. |
| RDB01583 | pAxCMtTA Shuttle vector to generate rAd expressing E. coli Tet-inducible tTA gene |
| RDB01584 | pAxTetZ Shuttle vector to generate rAd expressing tet-inducible lacZ |
| RDB01708 | pAx1-RxNtTA Recombinant adenoviral vector expressing NtTA |
| RDB01709 | pAx1-Tet NtTA Shuttle vector to generate rAd expressing NtTA |
| RDB01710 | pAx1-CA-NtTA Shuttle vector to generate rAd expressing NtTA |
| RDB01711 | pAx1-CM-NtTA Shuttle vector to generate rAd expressing NtTA |
| RDB06012 | pAdex TRE-SwaI Shuttle vector to generate rAd with Tet-responsive element (TRE) promoter |
| RDB01574 | AxTetZ Recombinant adenovirus expressing Tet-inducible lacZ gene |
| RDB01730 | Ax1-RxNtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01731 | Ax1-Tet NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01732 | Ax1-CA-NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB01733 | Ax1 CM-NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
| RDB02351 | Ax1 CM-NtTA Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal |
Luciferase
| Catalog no. | Name of resource | Type |
|---|---|---|
| RDB03988 | pCMVluc+ Expression vector of firefly luciferase |
 |
| RDB06411 | pGL3-RSV Expression vector of firefly luciferase, RSV promoter |
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| RDB03989 | pCAluc+ Expression vector of firefly luciferase |
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| RDB02443 | pCAcc-Luc+ Expressing luciferase under the control of CAG promoter |
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| RDB02444 | pLRICALuc+ Shuttle vector to generate recombinant adenovirus |
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| RDB02968 | pTcf7wtluc Reporter plasmid carring TCF-binding consensus sequence |
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| RDB02969 | pTcf7mtluc Reporter plasmid carring TCF-binding consensus sequence |
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| RDB07031 | ptk-luc Reporter construct with luciferase expression under tk promoter |
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| RDB07032 | pFGF4tk-luc Reporter construct with luciferase expression under FGF4 and tk promoter |
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| RDB02453 | AxCA Luc+ Recombinant adenovirus expressing luciferase gene |
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| RDB04026 | pKM2L Reporter construct with Renilla luciferase gene |
 |
| RDB05549 | pKM2L-pvTK HSV herpes simplex virus thymidine kinase (HSV-TK) |
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| RDB05550 | pKM2L-pvSV40 SV40 simian virus 40 early enhancer/promoter region (SV40) |
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| RDB05551 | pKM2L-pvCMV CMV cytomegalovirus immediate enhancer/promoter (CMV) |
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| RDB06408 | pRL-CMV mut1 Expression vector of Renilla luciferase, mutated CMV promoter |
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| RDB06409 | pRL-CMV mut2 Expression vector of Renilla luciferase, mutated CMV promoter |
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| RDB06410 | pRL-RSV Expression vector of Renilla luciferase, RSV promoter |
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LacZ
| Catalog no. | Name of resource |
|---|---|
| RDB06072 | pCMFlag_lacZ Expression vector of lacZ |
| RDB07081 | pCMFlag_lacZ Expression vector of lacZ |
| RDB01676 | pxCANLacZ A plasmid expressing a beta-galactosidase tagged with a NLS |
| RDB01870 | pCAZ 2 E. coli LacZ genomic DNA |
| RDB02037 | pLCAZ2 LacZ clone |
| RDB01680 | pCALNLZ A control plasmid to introduce LacZ gene into mammalian chromosome |
| RDB03380 | pAxCAiLacZit Shuttle vector to generate rAd harbouring E. coli LacZ |
| RDB02104 | pAxCAZ3 Shuttle vector to generate rAd expressing E. coli lacZ |
| RDB01589 | pAxCEAprZ Shuttle vector to generate rAd expressing E. coli lacZ gene driven by CEA promoter |
| RDB07061 | pAxEFFlag_LacZit2 Shuttle vector to generate recombinant adenovirus |
| RDB01699 | pRx nZ ires Neo Nls (nuclear localization signal), lacZ, ires, neo |
| RDB02976 | pMFGlacZ Shuttle vector to generate recombinant retrovirus cexpressing E. coli lacZ |
| RDB02039 | pAxPSZ Shuttle vector to generate rAd expressing E. coli lacZ |
| RDB01590 | pAxRxZ “Shuttle vector to generate rAd expressing E. coli lacZ gene driven by Rx promoter |
| RDB01503 | pAACAZ AAV vector expressing E. coli lacZ |
| RDB02038 | AxPSZ Recombinant adenovirus expressing E. coli lacZ regulated by human PSA promoter |
| RDB02726 | AxCALacZ Recombinant adenovirus expressing beta-galactosidase under the control of CAG promoter |
| RDB01750 | AxCALNLNZ Recombinant adenovirus expressing E. coli lacZ |
| RDB03211 | AxCANLacZ Recombinant adenovirus expressing E. coli lacZ |
| RDB02036 | AxCAZ2-F/K20 Recombinant adenovirus expressing lacZ regulated by CAG promoter. Fiber-mutant, F/K20 |
| RDB02196 | AxCAZ3 Recombinant adenovirus expressing E. coli lacZ regulated by CAG promoter |
| RDB02172 | AxCAZ3 F/asK21MSH beta Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/asK21MSH beta |
| RDB02175 | AxCAZ3 F/gsK21MSH beta Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/gsK21MSH beta |
| RDB02170 | AxCAZ3 F/gsMSH beta Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/gsMSH beta |
| RDB02258 | AxCAZ3-F/540S Recombinant adenovirus expressing E. coli lacZ |
| RDB02240 | AxCAZ3-F/asAT Recombinant adenovirus expressing E. coli lacZ |
| RDB02230 | AxCAZ3-F/asGRP Recombinant adenovirus expressing E. coli lacZ |
| RDB02231 | AxCAZ3-F/asGRP (4th) Recombinant adenovirus expressing E. coli lacZ |
| RDB04375 | AxCAZ3-F/asGRP27 Recombinant adenovirus expressing E. coli lacZ |
| RDB04376 | AxCAZ3-F/asGRP27NM Recombinant adenovirus expressing E. coli lacZ |
| RDB02165 | AxCAZ3-F/asMSH alpha Recombinant adenovirus expressing lacZ. Fiber-mutant, F/asMSH alpha mutant |
| RDB02236 | AxCAZ3-F/asSec Recombinant adenovirus expressing E. coli lacZ |
| RDB02237 | AxCAZ3-F/asSec (4th) Recombinant adenovirus expressing E. coli lacZ |
| RDB02194 | AxCAZ3-F/RGD Recombinant adenovirus expressing E. coli lacZ. Fiber-mutant, F/RGD |
| RDB03937 | AxCAZ3-F40STAA Recombinant adenovirus expressing E.coli lacZ |
| RDB03954 | Ax3CAZ3-F405hiRGD Recombinant adenovirus expressing E. coli lacZ |
| RDB03931 | Ax3CAZ3-F40SthiRGD Recombinant adenovirus expressing E. coli lacZ |
(GRP0037e 2004.08.22 T.M.)
2019.11.02