Conditional Gene Expression
Cre-loxP system
Vector backbone
| RDB15114 | pBSIISK-hFLEX Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch. |
| RDB04397 | pUSwS A cassette plasmid for inserting DNA between two loxP S sequences in the same orientation |
| RDB04396 | pUVwV A cassette plasmid for inserting DNA between two loxP V sequences in the same orientation |
| RDB03446 | pUAd5LCALNL(W1975C23R) Shuttle vector to generate recombinant adenovirus |
| RDB01862 | pCALNL5 A cassette to introduce cDNA into mammalian chromosome |
| RDB01679 | pCALwL A cassette plasmid to insert cDNA into downstream CAG promoter |
| RDB01677 | pULwL A cassette plasmid to insert stuffer DNA between two loxP site |
| RDB01674 | pCALNLw A cassette to introduce cDNA into mammalian chromosome |
Cre expression
| RDB16797 | pAcGFP-NCre Mammalian expression vector encoding Cre recombinase tagged with AcGFP1. |
| RDB15053 | pK073.TRE-Dre-WPRE (Supernova) Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase. |
| RDB15050 | pK170.AAV-TRE-Cre-WPRE (Supernova) Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. |
| RDB15043 | pK031.TRE-Cre (Supernova) Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. |
| RDB14533 | pCS2-OlCre Expression vector of P1 phage Cre recombinase, codon-optimized for Oryzias latipes (japanese medaka). |
| RDB12117 | pPyCAG-CreGR-IB Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. Blasticidin S resistance. |
| RDB09596 | pPyCAG-Cre-IRESpuropA Expression vector of Cre recombinase. Puromycin resistance. |
| RDB08998 | pCAGGS-Cre Expression clone of Cre recombinase. |
| RDB07106 | pPyCAG-CreGR-ID Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. HisD resistance. |
| RDB06073 | pKM2C Reporter construct with Cre recombinase gene |
| RDB01748 | AxCANCre Recombinant adenovirus expressing recombinase Cre with nuclear localization signal |
| RDB01675 | pxCANCre A plasmid expressing the recombinase Cre tagged with a nls |
Positive control vector
Plasmid clone pxCALNLEYFP (RDB06232) can be used for a positive control of Cre-mediated gene regulation. The pxCALNLEYFP contains CAG promoter, loxP-Neo-loxP stuffer sequence and coding region of EYFP. In this example, the pxCALNLEYFP was transfected to COS1 cells with or without pxCANCre (RDB01675), which expresses Cre recombinase.
Expression of YFP was detected in cells transfected pxCALNLEYFP with pxCANCre (right) but not pCALNLEYFP alone (left).
| RDB16747 | pCALNL5:mCherry Cre recombinase inducible expression cassette of mCherry. |
| RDB06232 | pxCALNLEYFP Expression vector of EYFP |
| RDB03347 | AxCALNLEGFP (forward) Recombinant adenovirus harboring enhanced green fluorescent protein cDNA |
| RDB03262 | pAxCALNLEYFP (reverse) Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA |
| RDB03261 | pAxCALNLEYFP (forward) Shuttle vector for rAd harboring enhanced yellow fluorescent protein cDNA. |
| RDB03260 | pAxCALNLEGFP (reverse) Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA |
| RDB03259 | pAxCALNLEGFP (forward) Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA. |
| RDB03252 | pAxCALNLBFP (reverse) Shuttle vector for rAd harboring blue fluorescent protein cDNA |
| RDB03251 | pAxCALNLBFP (forward) Shuttle vector for rAd harboring blue fluorescent protein cDNA. |
| RDB01750 | AxCALNLNZ Recombinant adenovirus expressing E. coli lacZ |
| RDB01680 | pCALNLZ A control plasmid to introduce LacZ gene into mammalian chromosome |
2025.05.03 (T.M.)
