Distribution of CoralHue™ Fluorescent Protein Resources

With the generosity of Medical & Biological Laboratories Co., Ltd. (MBL), 88 items of CoralHue™ fluorescent protein resources were deposited to RIKEN BioResource Research Center (BRC). On September 28, 2020, we will start distributing these resources as they are ready to be shipped. For details of each resource, please refer the attached table and the home page. These resources will be distributed to not-for-profit academic research conducted by universities and national/public research institutions at normal fees without license fee to MBL. In addition, we will distribute these resources to commercial entities and to for-profit-use at non-profit organizations after these users obtain an approval from MBL in advance.

These resources include large numbers of functional fluorescent protein resources developed by Dr. Atsushi Miyawaki Laboratory, RIKEN Center for Brain Science (CBS), such as (1) fluorescent proteins with various wavelength characteristics, (2) photoconversion type that changes color by light irradiation, and (3) photochromism type that can control light emission and extinction and can be used for super-resolution imaging. There are also a series of resources that can visualize various subcellular locations.

We are looking forward to receiving many requests for these resources from you.

For questions regarding the fluorescent protein resources, please refer at the attached table or the home page, and contact the Gene Engineering Division (dnabank.brc@riken.jp).

CoralHue™ Basic Fluorescent Proteins

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Kusabira-Cyan Kikuchi A et al. Crystal structure of a new cyan fluorescent protein and its hue-shifted variants. Biochemistry 48, 5276-83 (2009) (PMID: 19402703)
KCy1	Dimer	453	486	RDB18310

Midoriishi-Cyan Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
MiCy1	Dimer	472	495	RDB18265
mMiCy1	Monomer	470	496	RDB18289
hmMiCy1	Monomer	470	496		RDB18290	RDB18291	RDB18292	RDB18288

Umikinoko-Green Tsutsui H et al. Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods. 5, 683-685 (2008) (PMID: 18622396)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
mUKG1	Monomer	483	499	RDB18306
hmUKG1	Monomer	483	499	RDB18307	RDB18308	RDB18309

Azami-Green Karasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
AG	Tetramer	492	505	RDB18244
mAG1	Monomer	492	505	RDB18246	RDB18247	RDB18248
hmAG1	Monomer	492	505	RDB18249	RDB18250	RDB18251	RDB18252	RDB18245
hmAG407	Monomer	407	498	RDB18328

Kusabira-Orange Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
KO1	Dimer	548	561	RDB18253
mKO1	Monomer	548	559	RDB18258	RDB18259	RDB18260
mKO2	Monomer	551	565	RDB18299
hKO1	Dimer	548	561	RDB18254	RDB18255	RDB18256
hmKO1	Monomer	548	559	RDB18261	RDB18262	RDB18263	RDB18264	RDB18257
hmKO2	Monomer	551	565		RDB18300	RDB18301	RDB18302	RDB18298

Keima570 Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
dKeima570	Dimer	440	570	RDB18294
hdKeima570	Dimer	440	570	RDB18295			RDB18296	RDB18293

Keima-Red Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
dKeima-Red	Dimer	440	616	RDB18285
mKeima-Red	Monomer	440	620	RDB18280		RDB18281
hdKeima-Red	Dimer	440	616	RDB18286			RDB18287	RDB18284
hmKeima-Red	Monomer	440	620	RDB18282			RDB18283	RDB18279

Kaede (photoconversion type) Ando R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Kaede	Tetramer	508/572	518/580	RDB18241	RDB18242	RDB18243

Kikume Green-Red (photoconversion type) Habuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591) Tsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
KikGR1	Tetramer	507/583	517/593	RDB18272	RDB18273	RDB18274
mKikGR1	Monomer	505/580	517/591	RDB18304
hKikGR1	Tetramer	507/583	517/593	RDB18275	RDB18276	RDB18277	RDB18278	RDB18271
hmKikGR1	Monomer	505/580	517/591				RDB18305	RDB18303

Dronpa Green (photochromism type) Ando R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670)
Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
DG1	Monomer	503	518	RDB18267	RDB18268	RDB18269
hDG1	Monomer	503	518				RDB18270	RDB18266
DG3	Monomer	491	514	RDB18297

#	S1 vector: Vectors for subcloning
$	MC1 vector, MN1 vector: Expression vectors
*	MCLinker vector, MNLinker vector: Expression vectors with flexible linker (The linker sequence: TGNSADGGGGSGGSGGSGGGSTQG)

Organelle Targeting Vector Resources

Visualizing intracellular organs with fluorescent proteins in expression vector.

Abbr.	Plasma membrane	Mitochondria	Endoplasmic reticulum	Nucleoplasm	Beta-actin
mMiCy1 ⁽¹⁾		RDB18323
hKCy1 ⁽¹⁾				RDB18325
mAG1 ⁽²⁾	RDB18313	RDB18311	RDB18312		RDB18314
AG ⁽²⁾				RDB18315
mAG407 ⁽²⁾			RDB18326
mKO1 ⁽³⁾	RDB18318	RDB18316	RDB18317
hmKO1 ⁽³⁾					RDB18319
KO1 ⁽³⁾				RDB18320
hdKeima570 ⁽¹⁾				RDB18327
mKeima-Red ⁽¹⁾	RDB18322
hdKeima-Red ⁽¹⁾				RDB18324

References

(1)	Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
(2)	Karasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)
(3)	Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)

(2020.09.28 T.M.)

2023.09.06