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Cloning vectors

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Cloning vector

  • Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli.
    Miyazaki, K.
    Biotechniques 58 (2): 86-88 (2015). PubMed PMID 25652032.

    Catalog# Resource name Description
    RDB17947 pUC18K_ePAG2 Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).
    RDB17948 pUC18Z_ePAG2 Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).
    RDB18372 pUC18K_mPAG2 Cloning vector harboring PheS counterselection marker. Kanamycin resistance.
    RDB18373 pUC18Z_mPAG2 Cloning vector harboring PheS counterselection marker. Zeocin resistance.
    RDB18374 pUC18C_mPAG2 Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance.
  • A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.
    Motohashi, K.
    Sci. Rep. 9 (1): 6417 (2019). PubMed PMID 31015513.

    Catalog num. Clone name Description
    RDB17479 pCRT Plasmid vector for TA-cloning of PCR-products.
    RDB17481 pCRZero Plasmid vector for blunt-end cloning of PCR-products.
    RDB17480 pCRZeroT Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products.

  • Engineering FRET constructs using CFP and YFP.
    Shimozono, S., Miyawaki, A.
    Methods Cell Biol. 85: 381-393 (2008). PubMed PMID 18155471.

    Catalog num. Clone name Description
    RDB15126 pBS coupler 1 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
    RDB15127 pBS coupler 2 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
    RDB15128 pBS coupler 3 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
    RDB15129 pBS coupler 4 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
    RDB15130 pBS coupler 5 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
    RDB15131 pBS coupler 6 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
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    Catalog num. Clone name Description
    RDB03699 pURV19-10 Cloning vector based on pUC19 with EcoRV site.

2025.05.03 (T.M.)



 

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