Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

S-Myc-pRc/CMV (#RDB02140)

Expressing Myc-tagged fusion protein, CMV promoter.

Alternative name S-Myc, single Myc-tagged expression vector
Clone info. Expression vector. Expressing Myc-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37 C, LB+Amp
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
 
Sequence RDB02140z.seq
Additional map
Remarks, protocol and/or map (pdf) RDB02140.pdf

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02140 S-Myc-pRc/CMV DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The S-Myc-pRc/CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02140).

Reference section:

Takemoto, Y., Sato, M., Furuta, M., Hashimoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14003_A6Csp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14003_A6Csa.seq check
>02140_14003_A6Cs_1_CMV-Forward_C01_07.ab1
    1 ACGTTCCAAC CTCCCGCCCC ATTGACGGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
  121 TAATACGACT CACTATAGGG AGACCCAAGC TTCCATGGAG CAGAAGCTGA TCAGCGAGGA
  181 GGACCTGGCG GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT
  241 GCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC
  301 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA
  361 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG
  421 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG
  481 GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CGAGGGGGGA TCCCCACGCG
  541 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
  601 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
  661 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
  721 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
  781 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
  841 TTGTTCCAAA ACTGGGAACA ACACTCAACC CTATCTCGGG TCTATTCTTT TGATTTATAA
  901 GGGATTTTGC CGATTTCGGC CTATTGGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA
  961 CGCGAAATTT AACAAAATAT TAACGCTTAC AAATTAATTA TTTGCTTATA CAATCTTCCC
 1021 TGGTTTTTGG GGGCTTTTTC TGATTATCAC CGGGGGGTGG GGTACCGAGC TCGGAATTCT
 1081 GGTGGGAATG GTGGTGTCAG TTTAAGGGTG TGGAAAAGTC CCCCAGCTCC CCAGCAGGCA
1141 GAGGTTATGC AAAGCATGCC AATCCTCCCA TTAGGTCCGA CGCAGAACAC TCGAGAG
//
Primer: SV40pro_F_V2
Sequence file: RDB14003_A6Csb.seq check
>02140_14003_A6Cs_1_SV40pro_F_V2_D01_10.ab1
    1 TTCCCCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
  961 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GGCTGGGATG
 1021 ATCCTCCAGC GCGGGGATCT CATGCTGAGG TTCTTCGCCC ACCCCCAACT TGTTTATTGC
 1081 AGCTTATAAT GGCTTTACGA TAAGGCCATA GCCATTCACT AATTTCACAA ATTAAAGCAT
1141 TTTTTTTTCC ACCTGGCCAA TC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [link to RRC of NBRP]