Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab7-Q67L

Expression vector of mouse Rab7A constitutive active mutant, Q67L, fused with EGFP at N-terminus.

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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16878_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert Sequence file: RDB16878_A9A8a.seq
>D06060F7_A9A8_3_EGFP-C2_E06_15_ABI24.ab1     1 NNNNNNNNNN NNNAANCGCG NNCNCNTGGT CCTGCTGGAN TTCGTGACCG CCGCCGGGAT    61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGACCT CTAGGAAGAA   121 AGTGTTGCTG AAGGTCATCA TCCTGGGGGA CTCTGGTGTT GGAAAGACCT CTCTCATGAA   181 CCAGTATGTG AACAAGAAGT TCAGTAACCA GTACAAAGCC ACAATAGGAG CGGACTTTCT   241 GACCAAGGAG GTGATGGTGG ACGACAGACT TGTTACCATG CAGATCTGGG ACACAGCCGG   301 TCTAGAACGG TTCCAGTCTC TTGGTGTGGC CTTCTACAGA GGTGCAGATT GCTGTGTTCT   361 GGTGTTTGAT GTGACTGCCC CCAACACTTT CAAAACCCTC GACAGCTGGA GAGACGAGTT   421 TCTCATCCAG GCCAGCCCCC GGGATCCCGA GAACTTCCCT TTTGTTGTGT TGGGAAACAA   481 GATTGACCTG GAAAACAGAC AAGTGGCCAC AAAGAGGGCA CAGGCTTGGT GCTACAGCAA   541 AAACAACATT CCTTACTTCG AGACCAGTGC CAAGGAGGCC ATCAATGTGG AGCAGGCCTT   601 CCAGACAATT GCTCGGAATG CCCTTAAACA GGAAACAGAA GTGGAACTGT ACAATGAATT   661 CCCTGAACCC ATCAAACTGG ACAAGAATGA CCGGGCCAAG GCCTCCGCAG AAAGCTGCAG   721 TTGTTGAAAT CACTAGTGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGA   781 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA   841 AACCTCCCAC ACCTCNNCCN NAACCTGAAA CATAAAATGA ATGCNATTGT TGTTGTTAAC   901 NTNGTTTATT GCAGCTTATA ATGNNTACAA ATAAAGCAAT ANCATCACNA ATTTCACAAA   961 TAAAGCATTT TTTTTCNCNG CATTCNANNN GNNGNNTTGN NCNAAANNCN NCNANGNNNN 1021 NNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB16878_A9A8b.seq
>D06060F7_A9A8_3_SV40polyA-R2_F06_18_ABI24.ab1     1 NNNNNNNNNC NNNANNNANN NGTTAANNAC NACNATTGCA TTCNTTTTAT GTTTCNGGTT    61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT   121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA   181 ATTCACTAGT GATTTCAACA ACTGCAGCTT TCTGCGGAGG CCTTGGCCCG GTCATTCTTG   241 TCCAGTTTGA TGGGTTCAGG GAATTCATTG TACAGTTCCA CTTCTGTTTC CTGTTTAAGG   301 GCATTCCGAG CAATTGTCTG GAAGGCCTGC TCCACATTGA TGGCCTCCTT GGCACTGGTC   361 TCGAAGTAAG GAATGTTGTT TTTGCTGTAG CACCAAGCCT GTGCCCTCTT TGTGGCCACT   421 TGTCTGTTTT CCAGGTCAAT CTTGTTTCCC AACACAACAA AAGGGAAGTT CTCGGGATCC   481 CGGGGGCTGG CCTGGATGAG AAACTCGTCT CTCCAGCTGT CGAGGGTTTT GAAAGTGTTG   541 GGGGCAGTCA CATCAAACAC CAGAACACAG CAATCTGCAC CTCTGTAGAA GGCCACACCA   601 AGAGACTGGA ACCGTTCTAG ACCGGCTGTG TCCCAGATCT GCATGGTAAC AAGTCTGTCG   661 TCCACCATCA CCTCCTTGGT CAGAAAGTCC GCTCCTATTG TGGCTTTGTA CTGGTTACTG   721 AACTTCTTGT TCACATACTG GTTCATGAGA GAGGTCTTTC CAACACCAGA GTCCCCCAGG   781 ATGATGANCT TCAGCAACAC TTTCTTCCTA GAGGTCATGG ATCTGANTCC GGANTTGTAC   841 AGCTCGTCCA TGCCGANAGT GATCCCGGCG GCGGTCACGA ACTCCNGCNN NANNNTGTGA   901 TCGCGCTTCN CGNNNGGGNC TTTGCTCNNG NCNGNNTGGG NNNTCNNNNA NNGGNTGTCG 961 GGNANCANCA CGGGNNNNNN NNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB16878_A9A8c.seq
>D06060F7_A9A8_3_CMV_Forward_G06_21_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC   661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC   721 ACCCAGTCCG CCCTGAGCAA AGANCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN   781 TTCGTGANCG CCGCCGGGNT CACTCTCGGC ATGGNCGANC TGTACAAGTN CGGANTCAGA   841 TNCNTGANCT CTANGAAGAA AGTGTTGCTG AANGTCATCA TCCTGGGGNA NNNNNNNGNT   901 GNAAAGNNNN CTCNNATGNA NCNNGTATGT GNANNNNAAN TTCAGTAANN NGTNNNAAGN 961 NNCNNNNNNA NCNGNNNNTT NNGANCNNNN ANNNNNNNGN NNGNNNNANN NNNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB16878_A9A8d.seq
>D06060F7_A9A8_3_SV40pro_F_V2_H06_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNTTTTGN AAGNNCGATC AAGAGANAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN   781 CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG ANTTCTTCTG AGCGGGACTC TGGGGTTCGA ANTGACCGAC CAAGCGACGC   901 CCAACCTGNC ATCACGAGAT TTCNANNNCN NCGCCGCNTT CTATGAAANG NNNGGNNTCN   961 GNNNCNTTTT CCNGGNNNNN NGNNNGNNGA TNCTNNNNCG CNGGGNTNNN NNGNNNNNNN 1021 NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl