Please review the QC test results indicated by icon below as well as clone information before placing your order.

GEPRA-AA/ pT2KXIGdeltain

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish. Low-affinity verson of the GEPRA-B.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 付加的提供条件： Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15254_A7H9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F2 Sequence file: RDB15254_A7H9a.seq
>D04498G3-1_A7H9_EF1a-C_F2_C11_08_ABI24.ab1     1 GGTGGGGGTT AGGCGGTTCG CTCTCTTCCT CACCGGGTCT GCGGCGAGTT CTAGCTGAAG    61 CTTCCTGCAG GTCGACCGAT CCTGAGAACT TCAGGGTGAG TTTGGGGACC CTTGATTGTT   121 CTTTCTTTTT CGCTATTGTA AAATTCATGT TATATGGAGG GGGCAAAGTT TTCAGGGTGT   181 TGTTTAGAAT GGGAAGATGT CCCTTGTATC ACCATGGACC CTCATGATAA TTTTGTTTCT   241 TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT TTTCTTTTCA TTTTCTGTAA   301 CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATTTT TAAATTCACT TTTGTTTATT   361 TGTCAGATTG TAAGTACTTT CTCTAATCAC TTTTTTTTCA AGGCAATCAG GGTATATTAT   421 ATTGTACTTC AGCACAGTTT TAGAGAACAA TTGTTATAAT TAAATGATAA GGTAGAATAT   481 TTCTGCATAT AAATTCTGGC TGGCGTGGAA ATATTCTTAT TGGTAGAAAC AACTACACCC   541 TGGTCATCAT CCTGCCTTTC TCTTTATGGT TACAATGATA TACACTGTTT GAGATGAGGA   601 TAAAATACTC TGAGTCCAAA CCGGGCCCCT CTGCTAACCA TGTTCATGCC TTCTTCTCTT   661 TCCTACAGCT CCTGGGCAAC GTGCTGGTTG TTGTGCTGTC TCATCATTTT GGCAAAGAAT   721 TCCTCGACGG ATCCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC   781 CTGGTCGAGC TGCAGGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG   841 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC   901 GTGCCCTGGC CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT CAGCCGCTAC   961 CCCGACCACA TGAAAGCAGC ACGACTTTCT TTCAAGTCCC GTCATGCCCG AAGCTACGTC  1021 CAGGAGCGCA CCCATCCTCC TTCAAGGACG ACCGCACTAC CAGGACCGCG CGAGTTGAGG 1081 TTCGAGGCGA CACCTGGTGA CCGCATCGAG CCTGGATGGT CAATTCGAAC CTTCCA //
Primer: pAxCALNL_F1 Sequence file: RDB15254_A7H9b.seq
>D04498G3-1_A7H9_pAxCALNL_F1_D11_11_ABI24.ab1     1 ATTATCTATC TTATCATGTC TGGATCATCA TCGATGAATT AATTCGAGCT CCACCGCGGT    61 GGCGGCCGCT CTAGATGCAT GCTCGAGTTA CTTGTACAGC TCGTTCATGC CTTCAGTGAT   121 CCCGGCGGCG GTCAGGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT   181 GAACAGGGCG GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC   241 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CCGCCGTCCT CGATGTTGTG   301 GCGGATCTTG AAGTTGGCCT TGATGCCGTT CTTCTGCTTG TCGGCGGTGA TATAGACGTT   361 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC   421 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG   481 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG   541 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG   601 CAGGCCGTAG CCCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT   661 GCAGAGCAGC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC   721 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT   781 GAACAGCTCC TCGCCCTTGC TCACCATGAG CTCTTCATGT CCTTCAGAAT TCTCCAGCAT   841 TTCCTGAATG AGAGGTGGCA TTGATCCAGG AATTTCCATT TTCAAAGGTA ATTACACGTT   901 CGGCACCTTT CGCGCTGATG CTGCGGAGAT CTGTGATTTT CATTAAGATC TTTGGAAACA   961 TGTGAGGCTT TGCTGGGTCG TCGTTTTCTA ATGTAAATCT TTAGTGCTTT CCAGCAGTGG  1021 TTCTTCTAGC TTGTCTACTT TTGATGGTTT CCTCACGTCT GACCGGGTCT CAACAGATCA 1081 AACAGATTGC AACTGAGACG CCTTGCTTGT GTGGGTGATC CAATC //
Primer: RARbeta_LBD Sequence file: RDB15254_A7H9c.seq
>D04498G3_1_A7H9_RARbeta_LBD_H01_08_ABI08.ab1     1 CGGTAGGAAA AAGGTGGCAT TGATCCAGGA ATTTCCATTT TCAAGGTAAT TACACGTTCG    61 GCACCTTTCG CGCTGATGCT GCGGAGATCT GTGATTTTCA TTAAGATCTT TGGAAACATG   121 TGAGGCTTGC TGGGTCGTCG TTTTCTAATG TAAATCTTTA GTGCTTCCAG CAGTGGTTCT   181 TGGAGCTTGT CTACTTTTGT TGGTTCCTCA AGGTCCTGGC GGTCTCCACA GATTAAACAG   241 ATGGCACTGA GAAGGCCTGT TTCTGTGTCA TCCATTTCCA AAGGCAGGAG CTGGTTGGCA   301 AAGGTGAACA CAAGGTCAGT CAGAGGACCG AAGCCAGCAT TGTGCATCTG AGTTCGATTT   361 AGTGTAAGGC CATCAGCGAA AGTCATGGTG TCTTGCTCTG GGGTATAGGC GGTACAAATT   421 CTGAGAATCA AGATATCCAA GCAGGCGGCT TTGAGCAGGG TGATCTGGTC TGCGATGGTC   481 AGACCTGTGA AGCCCGGCAG ACGCTTGGCG AACTCCACGA TCTTAATAAT GCACTTGGTG   541 GCCAGCTCAC TGAATTTGTC CCAGAGGCCC AAGTCCAATC GGACCCGGTG GTCAGCGCTG   601 GAATTCGTGG TGTATTTACC CAGCTGGCAG AGTGAGGGAA AGGTTTCCTG GTGGGCTTTC   661 CGGATCTTCT CAGTGAGGTC GTCTAGCTCC GCTGTCATCT CATAGCTCTC ATGCATGCGG   721 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC   781 AGGGCGGACT GGGTGCTCAG GTAGTGGTTG TCGGGCAGGT TCACGGGGCC GTCGCCGATG   841 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCACGAT GTTGTGGCGG   901 ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG   961 CTGATGTAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CCGTCCTCCC TTGAAGTCGA  1021 TGCCCTTCAG CTCGAATGCG GTTCACAGGG TGTCGCCTCG AACTTTCACT CGGCGCGGGT  1081 CCTGTAGGTG CGGTCGTCCT GGAGGAGATT GGGTGCGCCT CCTGGACGTA GCCCTTTCGG 1141 GGCATGGAAC G //
Primer: EF1a-N_R2 Sequence file: RDB15254_A7H9d.seq
>D04498G3-1_A7H9_EF1a-N_R2_E11_14_ABI24.ab1     1 TTACGGGCAA GATTGCAGTT TGATTTGCAT TGGAAAGGGT CGCTGGCTTT TGTGTTACAC    61 GCCCCTTATT TGTGCTTGAT TAGATGATCC CCCTGCTCGA GGGCCCATCT GGCCTGTGTT   121 TCAGACACCA GGGAGTCTCT GCTCACGTTT CCTGCTATTT GCAGCCTCTC TATCAAGACT   181 AATACACCTC TTCCCGCATC GGCTGCCTGT GAGAGGCTTT TCAGCACTGC AGGATTGCTT   241 TTCAGCCCCA AAAGAGCTAG GCTTGACACT AACAATTTTG AGAATCAGCT TCTACTGAAG   301 TTAAATCTGA GGTTTTACAA CTTTGAGTAG CGTGTACTGG CATTAGATTG TCTGTCTTAT   361 AGTTTGATAA TTAAATACAA ACAGTTCTAA AGCAGGATAA AACCTTGTAT GCATTTCATT   421 TAATGTTTTT TGAGATTAAA AGCTTAAACA AGAATCTCTA GTTTTCTTTC TTGCTTTTAC   481 TTTTACTTCC TTAATACTCA AGTACAATTT TAATGGAGTA CTTTTTTACT TTTACTCAAG   541 TAAGATTCTA GCCAGATACT TTTACTTTTA ATTGAGTAAA ATTTTCCCTA AGTACTTGTA   601 CTTTCACTTG AGTAAAATTT TTGAGTACTT TTTACACCTC TGTCAAGAAC TCCTGGACAA   661 ACCTCTGACC TGTGTGGAAC AGAGTGGATA TGGGTGTCTG AACAGATATT CACGTCTTTT   721 GCAGATCAGA GGGCATTTCT GGTGTTGTGC AATGACCTGG GTCCAACAGG TAGGCGTACT   781 CGTATCCAAG AGCATTGTTG GACAGGAGGT AGTCTCCATT CCTATAAAGA GGAAGGAATG   841 GCACCATGTA GTAGCCGTCA TTGTGGCCAA TTGGTGCATT GGTACGTGGG TAGATGGACC   901 GGGGAGGCTG ATGAGTTCTT AGCCAGCGCT CAAGATGCTA GAAATGATGT AAGAAACAAA   961 TATGCAGATG AAAGTTTAAG ATAAGATAAA AAATTAAAAC AATAAATGCA ATTATGTTTT  1021 GTGTTTCTAT CCACCAATTA TTATCTCCAC GCAGTAAAGC CACCAAGAAT ATTTTTTGGT 1081 CCTTGCTCTG CAGCAAGTCA CCTTACATTG AATCTCAACT GTGA //
Primer: pT2KXIG#1 Sequence file: RDB15254_A7H9e.seq
>D04498G3_1_A7H9_pT2KXIG#1_G01_07_ABI08.ab1     1 AGGCCGCCAA TTAACCTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC    61 GCTCTAGAAC TAGTGGATCT GCTGGGCTTG CTGAAGGTAG GGGGTCAAGA ACCAGAGGTG   121 TAAAGTACTT GAGTAATTTT ACTTGATTAC TGTACTTAAG TATTATTTTT GGGGATTTTT   181 ACTTTACTTG AGTACAATTA AAAATCAATA CTTTTACTTT TACTTAATTA CATTTTTTTA   241 GAAAAAAAAG TACTTTTTAC TCCTTACAAT TTTATTTACA GTCAAAAAGT ACTTATTTTT   301 TGGAGATCAC TTCATTCTAT TTTCCCTTGC TATTACCAAA CCAATTGAAT TGCGCTGATG   361 CCCAGTTTAA TTTAAATGTT ATTTATTCTG CCTATGAAAA TCGTTTTCAC ATTATATGAA   421 ATTGGTCAGA CATGTTCATT GGTCCTTTGG AAGTGACGTC ATGTCACATC TATTACCACA   481 ATGCACAGCA CCTTGACCTG GAAATTAGGG AAATTATAAC AGTCAATCAG TGGAAGAAAA   541 TGGAGGAAGT ATGTGATTCA TCAGCAGCTG CGAGCAGCAC AGTCCAAAAT CAGCCACAGG   601 ATCAAGAGCA CCCGTGGCCG TATCTTCGCA GATCTGATCT AGAGGATCAT AATCAGCCAT   661 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG   721 AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC   781 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT   841 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCATCAT CGATGAATTA   901 ATTCGAGCTC CACCGCGGTG GCGGCCGCTC TAGATGCATG CTCGAGTTAC TTGTACAGCT   961 CGTTCATGCC CTTCAGTGAT CCCGGCGGCG GTCAGGAACT CCAGCAGGAC CATGTGATCG  1021 CGCTTCTCGT TGGGGTCTTT TGAACAGGCG GACTGGTAGC TCAGTAGTGG GTTGTCGGCA  1081 AGCAGCACGG TGGTCGCTGA TGGGGGTGTT CTGCTGGTAG TGATCCGCCG AGCCTGCACG 1141 GCCGGCCGAT CCCTTCGCGA ATGGTTTGTT GTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl