Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSKY5wRER1-0

Plasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiae

Distribution information

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Terms and conditions for distribution	Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003).
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提供条件	営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Cell. 14, 3605-3616, 2003)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08585_A5D1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TRP1pro_F Sequence file: RDB08585_A5D1a.seq
>08585_08585_A5D1_TRP1pro_F_F01_16.ab1     1 GCAAGGTGAA TAGCAACAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT    61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACGGA GGCCGCAGAA TGTGCTCTAG   121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC   181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC   241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA   301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG   361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC   421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG   481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC   541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC   601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA   661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT   721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA   781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT   841 CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT   901 CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA   961 GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAAGG GCGAAAAACC CGTCTATCAG  1021 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAGTTTT TTTGGGGTCG AGTGCCGTAA  1081 AGCACTTAAT TCGACCTTAA TGGAGCCCCC GATTTAAGCT GACTGGCGAG CGGCGAACGT 1141 GCGAGAACGC AAGGCAAGAA AAAG //
Primer: TDH3pro_F Sequence file: RDB08585_A5D1b.seq
>08585_08585_A5D1_TDH3pro_F_G01_19.ab1     1 TAGCCCTGTA GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA    61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAAG AACTTAGTTT CGAATAAACA   121 CACATAAACA GAATTCGCGG CCGCAGATCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC   181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG   241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC   301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG   361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC   421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC   481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC   541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC   601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC   661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC   721 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA   781 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC   841 ACTCTCGGCA TGGACGAGCT GTACAAGAGA TCTATGGATT ACGATAGCTC TGATACAATG   901 AACGGTGGTT CAAGTAACCC CTTAATCACT AAGATGAATA CAATGAAATT ATTATATCAA   961 CACTATTTGG ATAAAGTCAC TCCTCACGCT AAAGGAGAGG TGGGCTGTAT TGGTGGGTTT  1021 GTTATGTTTG TTTATGGTTT CGTATTACAA TGGCCCGAAC GCTGGGTATG TGATTTGCTA  1081 TGGGTCTAGT CTATTTTTTA TGGATTCACT TTTTAAGACG TTTTTTGACC CCAAAATTCG 1141 ATATATTGTT TCCGTTTACG //
Primer: EGFP-C Sequence file: RDB08585_A5D1c.seq
>08585_08585_A5D1_EGFP-C_H01_22.ab1     1 AAGGGATATC TCGGGCATGG ACGAGCTGTA CAAGAGATCT ATGGATTACG ATAGCTCTGA    61 TACAATGAAC GGTGGTTCAA GTAACCCCTT AATCACTAAG ATGAATACAA TGAAATTATT   121 ATATCAACAC TATTTGGATA AAGTCACTCC TCACGCTAAG GAGAGGTGGG CTGTATTGGG   181 TGGTTTGTTA TGTTTGTTTA TGGTTCGTAT TACAATGGCC GAAGGCTGGT ATGTGATTTG   241 TTATGGTCTA GGTCTATTTT TATTGAATCA ATTTTTAGCC TTTTTGACCC CAAAATTCGA   301 TATGTCCTTA CAGCAAGATG AAGAAAACAA CGAATTGGAA GCTGGAGAAA AATCAGAAGA   361 ATTCCGTCCA TTCATCAGAA GATTACCAGA GTTCAAATTC TGGTATAACA GCATTAGAGC   421 CACTGTCATT TCCCTCTTGT TGTCGCTATT TTCAATCTTC GATATTCCAG TATTTTGGCC   481 CATCTTATTG ATGTATTTCA TATTATTGTT TTTTTTAACT ATGAGAAGGC AGATTCAACA   541 TATGATAAAA TATAGATATA TACCCTTAGA TATCGGTAAG AAGAAATATT CTCATTCTTC   601 TAACTGAAGC TTTTGCACAA TTAGTTAACA CAGTGCTTGC CGAAAAGGAA AAATTTTTAA   661 ATATTAACAG AGTCTGTAGT TCTGATAGCG ATCTTCCTGG GAGTGATATA AAGTCTTTGG   721 ATGAGGCGAA GGAGAAACCA GAGGGGAAGG ATACCAAAAC AGAAGAATGA TAACAGTATG   781 CCGTTTTACC ACACTGGCGA ATAAATCTTC AATGACTTTG TAAGCTCGAA TATGCGCAAG   841 ATTTGACTTT GATATTTTGC ACTTTTTCGT CCTCACTTTC TACCTTTTTT TTTTTATGCC   901 TTAGTTGAAA AGATACGCGT ATCTAATTAT GTACTAATAG TCTTAATTTT ACATTACTTA   961 ATTAATCGAA ATGCAGCCTG TAAGGAGTTT CACAATCTTT CACGTTCGTT GAAAGGCTAG  1021 TTGTCTAAGT GCGCTGCCCT TTTAACTCTA CCATCATCAA AAGAAGAATC ATTGTCGTGG  1081 TCAATCATAA TATCAGCTTC GTCGTCTCGT CATCATCGAA CAATATTTCC TCGCTATCAT 1141 GTCGCAAATT ACGTTACGTT CTGTTCGCGA TAAATC //
Primer: Amp_R Sequence file: RDB08585_A5D1d.seq
>08585_08585_A5D1_Amp_R_A02_02.ab1     1 TTCGGTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA   181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC   241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC   301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA   361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT   421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT   481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC   541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA   601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC   661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT   721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA   781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG   841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAGATCACGA   901 CATTACTATA TATATAATAT AGGAAGCATT TAATAGAACA GCATCGTAAT ATATGTGTAC   961 TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAAGATAT TCTTTATTGA AAAATAGCTT  1021 GTCACCTTTA CGTACAATCT TGATCCGGGA GCTTTTCTTT TTTTGCCGAT AGAATTATTC  1081 GGTCGAAAAA AAGAAAATGA GAGGCCAGAG GAGGCATGTG ACTATGAGCA CGTGAGTATA 1141 CGTGATTAGC ACACAAAGGC CAGCCTTGGG GAGATAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.06.19

GNP_filter3_RDBDEP_html_230615.pl