Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAhIL12

Expression vector of human IL-12 (p40 and p35)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB03958_A8Ahp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330) Region: CAG pro,insert 5'(hIL12p40 5') Sequence file: RDB03958_A8Ahd.seq
>03958_03958_A8Ah_pCAG-F_V2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTG NGCGGNNCCG ANTCTGGGNN GCGCCGCCGC ACCCCCTCTA    61 GCGGGCGCGG GGCNANNNNN NNCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC   121 GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG   181 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG   241 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA   301 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCTAGACT GCCATGTGTC   361 ACCAGCAGTT GGTCATCTCT TGGTTTTCCC TGGTTTTTCT GGCATCTCCC CTCGTGGCCA   421 TATGGGAACT GAAGAAAGAT GTTTATGTCG TAGAATTGGA TTGGTATCCG GATGCCCCTG   481 GAGAAATGGT GGTCCTCACC TGTGACACCC CTGAAGAAGA TGGTATCACC TGGACCTTGG   541 ACCAGAGCAG TGAGGTCTTA GGCTCTGGCA AAACCCTGAC CATCCAAGTC AAAGAGTTTG   601 GAGATGCTGG CCAGTACACC TGTCACAAAG GAGGCGAGGT TCTAAGCCAT TCGCTCCTGC   661 TGCTTCACAA AAAGGAAGAT GGAATTTGGT CGACTGATAT TTTAAAGGAN CAGAAAGAAC   721 CCAAAAATAA GANCTTTCTA AGANGCGAGG CCNAGAATTA TTCTGGACGT TTCACCTGCT   781 GGTGGCTGAC GACAATCAGT ACTGATTTGA CATTCAGTGT CNAAAGCAGC AGANGCTCTT   841 CTGANCCCNN AGGGGTGANG TGCGGANCTG CTACACTCTC TGCAGANAGA NTCANANGGN   901 ANAACAANGA GTATGANTAC TCANNGGNNT GNCNNGANGA NAGTGNCTGC CCAGCTGNTG   961 ANGANANTCT GNCCNTTGAN GNNNTNNNNG NNGNCNTTCN NNAGCTNNNG TATNAAAACT 1021 ACNNNNNCNN CTTCTTCNTN ANGNNNNNCA TNNNNNTNNN NNNNNNN //
Primer: pBAC_lac_R (Pr0545) Region: insert 3'(beta-globin pA) Sequence file: RDB03958_A8Ahe.seq
>03958_03958_B2LlF_pBAC_lac_R_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTGG GCTGCAGGTC GAGGGNNCTC CATAAGAGAA    61 GAGGGACAGC TATGACTGGG AGTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT   121 GTAAGGAAAA TTTTAGGGAT GTTAAAGAAA AAAATAACAC AAAACAAAAT ATAAAAAAAA   181 TCTAACCTCA AGTCAAGGCT TTTCTATGGA ATAAGGAATG GACAGCAGGG GGCTGTTTCA   241 TATACTGATG ACCTCTTTAT AGCCAACCTT TGTTCATGGC AGCCAGCATA TGGGCATATG   301 TTGCCAAACT CTAAACCAAA TACTCATTCT GATGTTTTAA ATGATTTGCC CTCCCATATG   361 TCCTTCCGAG TGAGAGACAC AAAAAATTCC AACACACTAT TGCAATGAAA ATAAATTTCC   421 TTTATTANCC ANAAGTCANA TGCTCAAGGG GCTTCATGAT GTCCCCNNNN TTTTTGGCAG   481 AGNNAANAAG ATCCTTANGA ANNNNTCNNA NNNNNCNNNN NNCTATCNAT AGNCACTGCC 541 CGAATTCNNA AAGCATGAAN ANNNNNGNNN ANNTTGATTN TNANNNNNAN NNNNNNNNN //
Primer: RDB03958#1 (Pr0535) Region: insert mid(hIL12p40 3') Sequence file: RDB03958_A8Ahf.seq
>03958_03958_A8Ah_RDB03958#1_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NGNNNATCNG TACTGATTTG ACNTTCNGTG TCAAAAGCAG    61 CAGAGGCTCT TCTGACCCCC AAGGGGTGAC GTGCGGAGCT GCTACACTCT CTGCAGAGAG   121 AGTCAGAGGG GACAACAAGG AGTATGAGTA CTCAGTGGAG TGCCAGGAGG ACAGTGCCTG   181 CCCAGCTGCT GAGGAGAGTC TGCCCATTGA GGTCATGGTG GATGCCGTTC ACAAGCTCAA   241 GTATGAAAAC TACACCAGCA GCTTCTTCAT CAGGGACATC ATCAAACCTG ACCCACCCAA   301 GAACTTGCAG CTGAAGCCAT TAAAGAATTC TCGGCAGGTG GAGGTCAGCT GGGAGTACCC   361 TGACACCTGG AGTACTCCAC ATTCCTACTT CTCCCTGACA TTCTGCGTTC AGGTCCAGGG   421 CAAGAGCAAG AGAGAAAAGA AAGATAGAGT CTTCACGGAC AAGACCTCAG CCACGGTCAT   481 CTGCCGCAAA AATGCCAGCA TTAGCGTGCG GGCCCAGGAC CGCTACTATA GCTCATCTTG   541 GAGCGAATGG GCATCTGTGC CCTGCAGTTA GGATCGATCC TCTAGAGAAT TCCGCCCCCC   601 CNNNNNNNCC NNNNNNNNCT CCCCNNNNCC NAACGTTACT GGCCGAAGCC GCTTGGANTA   661 AGGNCGGNGT GCGTTTGTCT ATATGNNATT TTCCNCCANA TTGCCNNCNT TTGGNNNTGT   721 GAGGGCCCGN NANNCTGNCC CTGTCTTCTT GACNANCNTT CCTANGGGTC TTTCCCCTCT 781 CGCNAAAGGA ATGNNANGNT CTGNNNAATG NNNNNNANNA ANNANNNNNN NNNN //
Primer: IRES-F (Pr0036) Region: insert mid(hIL12p35) Sequence file: RDB03958_A8Ahg.seq
>03958_03958_A8Ah_IRES-F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTTN NGTCGANGTT ANNANACGTC TAGGCCCCCC GAACCACGGG    61 GACGTGGTTT TCNNNNNNNN NNNNNNATGA TAAGCTTGCC ACGATCCGGC TCTAGACTCA   121 NNATGTGTCC AGCGCGCAGC CTCCTCCTTG TGGCTACCCT GGTCCTCCTG GACCACCTCA   181 GTTTGGCCAG AAACCTCCCC GTGGCCACTC CAGACCCAGG AATGTTCCCA TGCCTTCACC   241 ACTCCCAAAA CCTGCTGAGG GCCGTCAGCA ACATGCTCCA GAAGGCCAGA CAAACTCTAG   301 AATTTTACCC TTGCACTTCT GAAGAGATTG ATCATGAAGA TATCACAAAA GATAAAACCA   361 GCACAGTGGA GGCCTGTTTA CCATTGGAAT TAACCAAGAA TGAGAGTTGC CTAAATTCCA   421 GAGAGACCTC TTTCATAACT AATGGGAGTT GCCTGGCCTC CAGAAAGACC TCTTTTATGA   481 TGGCCCTGTG CCTTAGTAGT ATTTATGAAG ACTTGAAGAT GTACCAGGTG GAGTTCAAGA   541 CCATGAATGC AAAGCTTCTG ATGGATCCTA AGAGGCAGAT CTTTCTAGAT CAAAACATGC   601 TGGCAGTTAT TGATGAGCTG ATGCAGGCCC TGAATTTCAA CAGTGAGACT GTGCCACAAA   661 AATCCTCCCT TGAAGAACCG GATTTTTATA AAACTAAAAT CAAGCTCTGC ATACTTCTTC   721 ATGCTTTCAG AATTCGGGCA GTGACTATTG ATAGAGTGAC GAGCTATCTG AATGCTTCCT   781 AAGGATCTTT TTCCCTCTGC CNAAAATTAT GGGGACATCA TGAAGCCCCT TGANCATCTG   841 ACTTCTGGCT AATAAAGGAA ATTTATTTTC NTTGCAATAG TGTGNTGGAN NTTTTTGNGT   901 CTCTCACTCG GAAGGANATA TGGGAGGGCA AATCATTTNA AACATCANAA NGAGTANTTG   961 GTTTANAGTT NNNCANCATA TGCCNNTATG CTGGCTGNCN TGNNNNANNN NNNNCTATNA 1021 NNANNTCNTC AGNATNNNNN NNNNCCCNNN NTNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,SV40 pA Sequence file: RDB03958_A8Ahh.seq
>03958_03958_B2LlF_SV40pro_ori_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT    61 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG   121 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG   181 TCTGGATCCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG   241 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC   301 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG   361 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGNAAAAAGG CCGCGTTGCT   421 GGCGTTTTTC CATANGCTCC GCCCCCCTGA NNAGCATNNN NNNAATNNNN NNNCAANNNN 481 NANGNGGNNA AACCCNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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