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pAxCAmIL12p40

Shuttle vector to generate rAd expressing mouse IL-12 p40

Catalog number RDB01416
Resource name pAxCAmIL12p40
Alternative name pAdex1CAmIL12p40
Clone info. Shuttle vector to generate recombinant adenovirus expressing mouse IL-12p40 gene.
Comment Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Mouse IL-12 p40 cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
Mouse IL-12 p40
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01416.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Clin. Invest., 102, 1807-1814 (1988).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Clin. Invest., 102, 1807-1814 (1988).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01416 pAxCAmIL12p40 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAxCAmIL12p40 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01416).

          Reference section:

          Yasuda, H., Nagata, M., Arisawa, K., Yoshida, R., Fujihira, K., Okamoto, N., Moriyama, H., Miki, M., Saito, I., Hamada, H., Yokono, K., Kasuga, M., Local expression of immunoregulatory IL-12p40 gene prolonged syngeneic islet graft survival in diabetic NOD mice. J. Clin. Invest., 102, 1807-1814 (1988). PMID 9819366.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB01416_B2L1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CAG_R1 (Pr0584)
          Region: CAG pro,insert 5'
          Sequence file: RDB01416_B2L1a.seq check
          >01416_01416_B2L1_3_CAG_R1_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNAN CGGGCGCGGG GCGNNNCGGT GCGGCGCCGG CAGGAAGGAA
             61 ATGGGCGGGG NGGGCCTTCG TGCGTCGCCG CGCCGCCGTC CCCTTCTCCC TCTCCAGCCT
            121 CGGGGCTGTC CGCGGGGGGA CGGCTGCCTT CGGGGGGGAC GGGGCAGGGC GGGGTTCGGC
            181 TTCTGGCGTG TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT
            241 TTCCTACAGC TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA
            301 TTAGACGGCA TCGCAGCTTG GATACACGCC GCCCACGTGA AGGCTGCCGA CCCCGGGGGT
            361 GGACCATCCT CTAGACTGCC ATGGGATGTC CTCAGAAGCT AACCATCTCC TGGTTTGCCA
            421 TCGTTTTGCT GGTGTCTCCA CTCATGGCCA TGTGGGAGCT GGAGAAAGAC GTTTATGTTG
            481 TAGAGGTGGA CTGGACTCCC GATGCCCCTG GAGAAACAGT GAACCTCACC TGTGACACGC
            541 CTGAAGAAGA TGACATCACC TGGACCTCAG ACCAGAGACA TGGAGTCATA GGCTCTGGAA
            601 AGACCCTGAC CATCACTGTC AAAGAGTTTC TAGATGCTGG CCAGTACACC TGCCACAAAG
            661 GAGGCGAGAC TCTGAGCCAC TCACATCTGC TGCTCCACAA GAANGAAAAT GGAATTTGGT
            721 CCACTGAAAT TTTAAAAAAT TTCAAAAACA AGACTTTCCT GAAGTGTGAA GCACCNAATT
            781 ACTCCGGACG GTTCACGTGC TCATGGCTGG TGCNAAGAAA CATGGNCTTG ANNTTCAACA
            841 TCNAGAGCAG TANCAGTTCC CNNNNNTCTC GGGCAGNGNN ATGTGGAATN GCGTCTCNGN
            901 CNGCNNANNA NNNNCACACN NGNNNNNAAN GGNNTNNNAN AANGTATTCN GTGNNCNNNN
          961 NNNNNN
          //
          Primer: pCAGGS_R1 (Pr0614)
          Region: beta-globin polyA,insert 3'
          Sequence file: RDB01416_B2L1b.seq check
          >01416_01416_B2L1_3_pCAGGS_R1_G03_21_ABI24.ab1
              1 ANNNNNNNNN NNNNNACNAT TGNNNTGNAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA
             61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG
            121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
            181 TGAGGAGTGA ATTATCCTGT CTTTAACAAA TTGGACTAAT CCGGATCCTA GGATCGGACC
            241 CTGCAGGGAA CACATGCCCA CTTGCTGCAT GAGGAATTGT AATAGCGATC CTGAGCTTGC
            301 ACGCAGACAT TCCCGCCTTT GCATTGGACT TCGGTAGATG TCTTCTCTAC GAGGAACGCA
            361 CCTTTCTGGT TACACCCCTC CTCTGTCTCC TTCATCTTTT CTTTCTTGCG CTGGATTCGA
            421 ACAAAGAACT TGAGGGAGAA GTAGGAATGG GGAGTGCTCC AGGAGTCAGG GTACTCCCAG
            481 CTGACCTCCA CCTGTGAGTT CTTCAAAGGC TTCATCTGCA AGTTCTTGGG CGGGTCTGGT
            541 TTGATGATGT CCCTGATGAA GAAGCTGGTG CTGTAGTTCT CATATTTATT CTGCTGCCGT
            601 GCTTCCAACG CCAGTTCAAT GGGCAGGGTC TCCTCGGCAG TTGGGCAGGT GACATCCTCC
            661 TGGCAGGACA CTGAATACTT CTCATAGTCC CTTTGGTCCA GTGTGACCTT CTCTGCAGAC
            721 AGAGACGCCA TTCCACATGT CACTGCCCGA NAGTCANGNG AACTGCTACT GCTCTTGATG
            781 TTGAACTTCA AGTCCATGTT TCTTTGCACC AGCCATGANC ACGTGAACCG TCCGGANTAA
            841 TTNGGTGCTT CACACTTCNN NAAAGTCTTG NTTTTTGAAA TTTTTTTAAA ATTTCANNGG
            901 ANNNAATTNC ATTTTTCCTN NTNGNNGNAN CANCANANGN NNNNNNNNCA NANNNNCGNC
          961 NNNCNNTNNN NNNNNNN
          //
          Primer: Amp_R (Pr0008)
          Region: AmpR pro,ITR,Ad5 psi
          Sequence file: RDB01416_B2L1c.seq check
          >01416_01416_B2L1_3_Amp_R_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGGTTATTG TCTCATGAGC GGANACATAT TTGAATGTAT
             61 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT
            121 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
            181 TCGTCTTCAA GAATTCCGGA TCCGGGCCAA TATGATAATG AGGGGGTGGA GTTTGTGACG
            241 TGGCGCGGGG CGTGGGAACG GGGCGGGTGA CGTAGTAGTG TGGCGGAAGT GTGATGTTGC
            301 AAGTGTGGCG GAACACATGT AAGCGACGGA TGTGGCAAAA GTGACGTTTT TGGTGTGCGC
            361 CGGTGTACAC AGGAAGTGAC AATTTTCGCG CGGTTTTAGG CGGATGTTGT AGTAAATTTG
            421 GGCGTAACCG AGTAAGATTT GGCCATTTTC GCGGGAAAAC TGAATAAGAG GAAGTGAAAT
            481 CTGAATAATT TTGTGTTACT CATAGCGCGT AATATTTGTC TAGGGCCGCG GGGACTTTGA
            541 CCGTTTACGT GGAGACTCGC CCAGGTGTTT TTCTCAGGTG TTTTCCGCGT TCCGGGTCAA
            601 AGTTGGCGTT TTATTATTAT AGTCAGCATC GATGAGCTTG ATAGCTTGGG CTGCAGGTCG
            661 AGGGATCTCC ATAAGAGAAG AGGGACAGCT ATGACTGGGA GTAGTCAGGA GAGGAGGAAA
            721 AATCTGGCTA GTAAAACATG TAAGGAAAAT TTTAGGGATG TTAAAGAAAA AAAATAACAC
            781 AAAACAAAAT ATAAAAAAAA TCTAACCTCN AGTCAAGGCT TTTTCTATGG AATAANGANN
            841 GGNCAGCNNG GGGCTGTTTC ATATACTGAT GANCTCTTTA TAGCCAACCT TNNTNCATGG
            901 NAGCCAGCAT ATGGGNATAT GNTGNNNNNN NTNNNNNNAA NNNNNNTTNN NNNGNTTNNA
          961 NNNNNTNNNC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Yasuda, H., Local expression of immunoregulatory IL-12p40 gene prolonged syngeneic islet graft survival in diabetic NOD mice. J. Clin. Invest., 102, 1807-1814 (1988). PMID 9819366.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl