Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab3A-Q81L

Expression vector of mouse Rab3A constitutive active mutant, Q81L, fused with EGFP at N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16871_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP, insert 5' (Q81L) Sequence file: RDB16871_A9A8a.seq
>D06060E9_A9A8_2_EGFP-C2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNCGNTC NCNTGGTCCT GCTGGANTTC GTGACCGCCG CCGGGNTCAC    61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCTTCCG CCACAGACTC   121 TCGCTATGGG CAGAAGGAGT CCTCAGACCA GAACTTCGAC TATATGTTCA AGATCCTGAT   181 CATTGGGAAC AGCAGCGTGG GCAAAACCTC GTTCCTCTTC CGCTACGCAG ATGACTCCTT   241 CACTCCAGCC TTTGTCAGCA CCGTTGGCAT AGACTTCAAG GTCAAAACCA TCTATCGCAA   301 CGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGG CTAGAGCGGT ACCGCACCAT   361 CACCACAGCC TATTACCGAG GCGCCATGGG CTTCATCCTA ATGTATGACA TCACCAATGA   421 GGAGTCATTT AATGCAGTGC AGGACTGGTC CACTCAGATC AAAACTTACT CGTGGGACAA   481 TGCCCAGGTG CTGCTGGTGG GAAACAAGTG TGACATGGAA GATGAGCGAG TGGTGTCCTC   541 AGAGCGTGGC CGGCAGCTGG CTGACCACCT GGGCTTTGAG TTCTTTGAGG CCAGCGCCAA   601 GGACAACATT AATGTCAAGC AGACGTTTGA ACGTCTGGTG GACGTGATCT GTGAGAAGAT   661 GTCAGANTCC CTGGATACTG CAGACCCTGC GGTCACCGGT GCCAAGCANG GCCCGCAGCT   721 CACCGACCAG CANGCGCCAC CTCATCANGA NTGTGCCTGC TGAAATCACT AGTGAATTCT   781 GNAGNCGANG GTANCGCGGG CCCGGGNNNC ACCGGATCTA GATAACNGAT CATAATCAGC   841 CNTANNNNNT TTGNANANGN TTNACTNGCT TTAAAAAANN NCCCNCNCCN CCCCCNGAAN 901 CTGAANNNTA AAATGAANGN NNNNNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert (Q81L) Sequence file: RDB16871_A9A8b.seq
>D06060E9_A9A8_2_SV40polyA-R2_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA GTTAANNANN ACAATTGCAT TCNTTTTATG TTTCNGGTTC    61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG   121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA   181 TTCACTAGTG ATTTCAGCAG GCACAATCCT GATGAGGTGG CGCCTGCTGG TCGGTGAGCT   241 GCGGGCCCTG CTTGGCACCG GTGACCGCAG GGTCTGCAGT ATCCAGGGAC TCTGACATCT   301 TCTCACAGAT CACGTCCACC AGACGTTCAA ACGTCTGCTT GACATTAATG TTGTCCTTGG   361 CGCTGGCCTC AAAGAACTCA AAGCCCAGGT GGTCAGCCAG CTGCCGGCCA CGCTCTGAGG   421 ACACCACTCG CTCATCTTCC ATGTCACACT TGTTTCCCAC CAGCAGCACC TGGGCATTGT   481 CCCACGAGTA AGTTTTGATC TGAGTGGACC AGTCCTGCAC TGCATTAAAT GACTCCTCAT   541 TGGTGATGTC ATACATTAGG ATGAAGCCCA TGGCGCCTCG GTAATAGGCT GTGGTGATGG   601 TGCGGTACCG CTCTAGCCCT GCTGTGTCCC AGATCTGCAG CTTGATCCTC TTGTCGTTGC   661 GATAGATGGT TTTGANCTTG AAGTCTATGC CAACGGTGCT GACAAAGGCT GGAGTGAANG   721 AGTCATCTGC GTAGCGGAAG ANGAACGAGG TTTTGCCCAC GCTGCTGTTC CCAATGATCA   781 GGANCTTGAA CATATAGTCG AAGTTCTGGT CTGANGANTC CTTCTGCCCA TAGCGAGANT   841 CTGTGGCGGA AGCCATGGAT CTGANTNCGG ANTTGNNCAG CTCGNCCATG NCNANANTGA   901 TNCCGGNGGN NGNCNCGAAC TCCAGCAGGA NCNTGTGNTC NCGCNTCNCG NNNGGNNCTT   961 TGCTCNNNNC GNNNNGGNNN NNCNNNTANN NNNTNNNNGG GNANCANCNN NGGNNNNNNN 1021 NNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP Sequence file: RDB16871_A9A8c.seq
>D06060E9_A9A8_2_CMV_Forward_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTGN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA   121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG   181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC   241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA   301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG   361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG   421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC   481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG   541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA   601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT   661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA   721 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG   781 ANTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGNCGA NCTGTACAAG TCCGGANTCA   841 GATCCATGGC TTCCGCCACA GACTCTCGCN ATGGGCAGAA NGANTCCTCA NANCAGAACT   901 TCGANNATAT GTTCNNGATN NNGATCATTG GGAANAGCAG CGNGGGNAAA ACNNCNTNCC   961 NNTTCCGNNN CNNAGANNNN NNCNTNNNNC CNNCNNTNNN CAGCNNNNNN NGNNNNNNNN 1021 NN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB16871_A9A8d.seq
>D06060E9_A9A8_2_SV40pro_F_V2_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG CTTTTGCAAG NNCGATCAAG AGANAGGNNG AGGNNCGTTT    61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC   601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA   661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAG   721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG NCGGCGAATG GGCTGANCGC   781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT   841 CTTGANGAGT TCTTCTGANC GGGNNTCTGG GGGTNCGANN TGACCGACCN ANCGACGCCC   901 NACCNGCCAT CACGANNNTT CGANNCCNNC GCNNCCNNCT ATGAAANGNT NGGNNTNCNN 961 NANCGNTTTN CNGGNNNNNC NNNNNNGNTG NNNNNNCNNN NNNGGGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl