Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab3A-T36N

Expression vector of mouse Rab3A constitutive negative mutant, T36N, fused with EGFP at N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16870_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP, insert 5' (S36N) Sequence file: RDB16870_A9A8a.seq
>D06060E8_A9A8_3_EGFP-C2_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNGN NCNCNTGGTC CTGCTGGANT TCGTGACCGC CGCCGGGNTC    61 ACTCTCGGCA TGNNNNANNT GTACAAGTCC GGACTCAGAT CCATGGCTTC CGCCACAGAC   121 TCTCGCTATG GGCAGAAGGA GTCCTCAGAC CAGAACTTCG ACTATATGTT CAAGATCCTG   181 ATCATTGGGA ACAGCAGCGT GGGCAAGAAT TCGTTCCTCT TCCGCTACGC AGATGACTCC   241 TTCACTCCAG CCTTTGTCAG CACCGTTGGC ATAGACTTCA AGGTCAAAAC CATCTATCGC   301 AACGACAAGA GGATCAAGCT GCAGATCTGG GACACAGCAG GGCAAGAGCG GTACCGCACC   361 ATCACCACAG CCTATTACCG AGGCGCCATG GGCTTCATCC TAATGTATGA CATCACCAAT   421 GAGGAGTCAT TTAATGCAGT GCAGGACTGG TCCACTCAGA TCAAAACTTA CTCGTGGGAC   481 AATGCCCAGG TGCTGCTGGT GGGAAACAAG TGTGACATGG AAGATGAGCG AGTGGTGTCC   541 TCAGAGCGTG GCCGGCAGCT GGCTGACCAC CTGGGCTTTG AGTTCTTTGA GGCCAGCGCC   601 AAGGACAACA TTAATGTCAA GCAGACGTTT GAACGTCTGG TGGACGTGAT CTGTGAGAAA   661 ATGTCAGAGT CCCTGGATAC TGCAGACCCT GCGGTCACCG GTGCCAAGCA GGGCCCGCAG   721 CTCACCGACC AGCAGGCGCC ACCTCATCAG GANTGTGCCT GCTGAAATCA CTAGTGAATT   781 CTGCAGTCGA CGGTACCGCG GGCCCGGGNT CCACCGGATC TAGATAACTG ATCATAATCA   841 GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCNNNNCACC TCCCCCCTGN   901 ACCTGAAACA TAAAATGAAT GCNATTGTTG TTGTNNNCTT GTTTANTGCA NCTTATAANG   961 GTTANAAATA AAGCAATANC ATCNCAAATT TCACAANTAA NNCNNTTTTT TTNNNNNNNN 1021 NN //
Primer: SV40polyA_R2 (Pr0623) Region: insert (S36N) Sequence file: RDB16870_A9A8b.seq
>D06060E8_A9A8_3_SV40polyA-R2_F02_17_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNN NAGTTAANNA CAACNATTGC ATTCNTTTTA TGTTTCNGGT    61 TCNGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC   121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACTGCAG   181 AATTCACTAG TGATTTCAGC AGGCACAATC CTGATGAGGT GGCGCCTGCT GGTCGGTGAG   241 CTGCGGGCCC TGCTTGGCAC CGGTGACCGC AGGGTCTGCA GTATCCAGGG ACTCTGACAT   301 TTTCTCACAG ATCACGTCCA CCAGACGTTC AAACGTCTGC TTGACATTAA TGTTGTCCTT   361 GGCGCTGGCC TCAAAGAACT CAAAGCCCAG GTGGTCAGCC AGCTGCCGGC CACGCTCTGA   421 GGACACCACT CGCTCATCTT CCATGTCACA CTTGTTTCCC ACCAGCAGCA CCTGGGCATT   481 GTCCCACGAG TAAGTTTTGA TCTGAGTGGA CCAGTCCTGC ACTGCATTAA ATGACTCCTC   541 ATTGGTGATG TCATACATTA GGATGAAGCC CATGGCGCCT CGGTAATAGG CTGTGGTGAT   601 GGTGCGGTAC CGCTCTTGCC CTGCTGTGTC CCAGATCTGC AGCTTGATCC TCTTGTCGTT   661 GCGATAGATG GTTTTGACCT TGAAGTCTAT GCCAACGGTG CTGACAAAGG CTGGAGTGAA   721 GGAGTCATCT GCGTAGCGGA AGAGGAACGA ATTCTTGCCC ACGCTGCTGT TCCCAATGAT   781 CAGGATCTTG AACATATAGT CGAAGTTCTG GTCTGANGAN TCCTTCTGCC CATAGCGAGA   841 GTCTGTGGCG GAAGCCATGG ATCTGANNCC GGANNTGTAC AGCTCGTCCA TGCNGANANT   901 GATCCNGGCG GCGGTCNNGA ACTCCAGCAN GNNNNTGTGA TCGCGCTTCN CNTTGGGGTC   961 TTTGCTCNNN NCNNANNGGG NGNNCNNNTA NNGGNTTGNN NGGCANCAGC NCGGGGNCNN 1021 NNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP Sequence file: RDB16870_A9A8c.seq
>D06060E8_A9A8_3_CMV_Forward_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA   121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA   181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA   241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT   361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG   421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG   601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC   661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA   721 CCCAGTCCGC CCTGANCAAA GANCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT   781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGNCGANCT GTACAAGTCC GGANTCAGAT   841 CCATGGCTTC CGCCACAGAC TCTCGCTATG GGCAGAANGA NNNCNCAGAN NAGAACTTCG   901 ANTATATGTN CAANATNNNG ATCATTGGGA ACAGCAGCGN GGGNNAGANN NCGTNCCNNT   961 NNCGNNACGC ANANGANNNN NNCNNNCCNG CNNTTNNNAN CNNNNNTNNG NNNNNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB16870_A9A8d.seq
>D06060E8_A9A8_3_SV40pro_F_V2_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNGGNTTTTG NAAGNNCGAT CANNGAGACA GGATGAGGAT    61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA   601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAANA NCTTGNCGGC GAATGGGCTG   781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC   841 GCCTTCTTGA NGAGTTCTTC TGANCGGGNN TCTGGGGNNC GAAATGACCG ACCAAGCGAN   901 GCCNANCCTG NCNTCNCGAN NNTTCNATTC CACCGCCGCC TNCNNTNNNN NNNNGGCTTC 961 NGNANNCNTT TNNNNGNNNN NNGGCTNNNN GATNCNNNCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl