Please review the QC test results indicated by icon below as well as clone information before placing your order.

NPY-mK-GO/pcDNA3.1(+)

Neuropeptide Y (NPY)-tagged fluorescent timer protein, monomeric Kusabira Green Orange (mK-GO).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A. (2010) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Mol. Biol. Cell 21 (1): 87-94. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15245_A7H9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert 5'(NPY,mK-GO 5') Sequence file: RDB15245_A7H9g.seq
>D04498F3-2_A7H9_CMV-Forward_D08_11_ABI24.ab1     1 NNNNNNNNNN NNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT   121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTAGCCAC CATGCTAGGT   181 AACAAGCGAC TGGGGCTGTC CGGACTGACC CTCGCCCTGT CCCTGCTCGT GTGCCTGGGT   241 GCGCTGGCCG AGGCGTACCC CTCCAAGCCG GACAACCCGG GCGAGGACGC ACCAGCGGAG   301 GACATGGCCA GATACTACTC GGCGCTGCGA CACTACATCA ACCTCATCAC CAGGCAGAGA   361 TATGGAAAAC GATCCAGCCC AGAGACACTG ATTTCAGACC TCTTGATGAG AGAAAGCACA   421 GAAAATGTTC CCAGAACTCG GCTTGAAGAC CCTGCAATGT GGACGGTACC GCGGGCCCGG   481 GATCCGATGG TGAGTGTGAT TAAACCAGAG ATGAAGATGA GGTACTACAT GGACGGCTCC   541 GTCAATGGGC ATGAGTTCAC AATTGAAGGT GAAGGCACAG GCAGACCTTA CGAGGGACAT   601 CAAGAGATGA CACTACGCGT CACAATGGCC GAGGGCGGGC CAATGCCTTT CGCGTTTGAC   661 TTAGTGTCAC ACGTGTTCTG TTACGGCCAC AGAGTATTTA CTAAATATCC AGAAGAGATA   721 CCAGACTATT TCAAACAAGC ATTTCCTGAA NGCCTGTCAT GGGAAAGGTC GTTGGAGTTC   781 GAAGANGGTG GGTCCGCTTC AGTCAGTGCG CATATAAGCC TTAGAGGAAA CACCTTCTAC   841 CACNAATCCN AATTTACTGG GGTTNANTTT CCTGNNNATN GNCCTATCAT GNNAAANCNA   901 AGNGTTGNNT GGGANCNNTC AACCNANNAA NNTACTGNNN NNNNNNGNNT NCTNNANNNN 961 NGATNNTNNN NNNNNNN //
Primer: bGH_rev2 (Pr0606) Region: bGHpA,insert 3'(mK-GO) Sequence file: RDB15245_A7H9h.seq
>D04498F3-2_A7H9_BGH_rev2_E08_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNGGNNGN NNNNNNNNNN GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG   121 TGCTGGATAT CTGCAGAATT CTTAGGAATG AGCTACTGCA TCTTCTACCA GCTCAGTAAT   181 GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCCGATGTAA TGGTCTCCTG GCATTTCAAG   241 AATCTCTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT TTGTGATTGC CGCCTCCTTC   301 AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG TCGCTGGCAG TAATTTTCTC   361 GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA GGACCATCGG CAGGAAAGTT   421 AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT CTAAGGCTTA TATGCGCACT   481 GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT TCCCATGACA GGCCTTCAGG   541 AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT TTAGTAAATA CTCTGTGGCC   601 GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAAGGC ATTGGCCCGC CCTCGGCCAT   661 TGTGACGCGT AGTGTCATCT CTTGATGTCC CTCGTAAGGT CTGCCTGTGC CTTCACCTTC   721 AATTGTGAAC TCATGCCCAT TGACGGANCC GTCCATGTAG TACCTCATCT TCATCTCTGG   781 TTTAATCACA CTCACCATCG GATCCCGGGC CCGCGGTACC GTCCACATTG CANGGNCTTC   841 NAGCCNAGTT CTGGGAACAT TTTCTGTGCT TTCTCTCATC NAGANGNCTG AAATCAGTGT   901 CTCTGGGCTG GATNGNTTTN NNTATCTCTG CNNGGNNNNN ANNNNGANGN ANNNNCNCAN 961 CGCCNANNAN TATCNNGNNN NTNNNNNNNN NCNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB15245_A7H9i.seq
>D04498F3-2_A7H9_SV40pro_F_F08_17_ABI24.ab1     1 CNNNNNNNNN NNNNNNNNNN NNNNGTGNNG NNGNTTTTTT GGNNGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GANTCATCGA CTGTGNNCGG CTGGGTGTGG CGGANCGCTA   781 TCAGGACATA NCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AANGGGCTGA   841 NCGCTTCCTC GNGCTTTACN GTATCGCCGC TCCCGATTCG CAGCGCATCG CCNTCTATCG   901 CCTTCTTGNN GAGTNCTTNN NNNCGGGNNT NNNGGGGNTC NAAATGNNCG ANNNANNGAC 961 NCCNNANCNG NNNTCNCNNN NNTNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.02.16

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