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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

NFATc4(DN)/pCAGEN

Expression clone of mouse Nfatc4 dominant negative form

Catalog number RDB12859
Resource name NFATc4(DN)/pCAGEN
Clone info. The plasmid expressing dominant-negative (DN) form of mouse NFATc4 under the control of the CAG promoter.
Comment Encoding 1-191aa of mouse Nfatc4. Expression was confirmed by the depositor with anti-NFATc4 antibody.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nfatc4 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
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External Database
Mouse Nfatc4
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12859 NFATc4(DN)/pCAGEN DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12859_A4Iop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB12859_A4Ioa.seq check
          >RDB12859_1_pAxCA_F1_E10_13.ab1
              1 CATGGAGAAA ACATTCTGGC TCAATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
             61 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA CCATGGGGGC
            121 CGCAAGCTGC GAGGATGAGG AGCTGGAATT TAAGCTGGTG TTCGGAGAGG AAAAGGAGCC
            181 CCCCCCGCTG GGCCCTGGGG GTCCTGGGGA AGAGCTGGAC TCAGAGGATA CCCCTCCATG
            241 CTGTCGTTTG GCCCTGGGGG AACCCCTTCC TTATGGTGCT GCCCCTATTG GGATTCCCCG
            301 ACCCCCACCC CCTCGGCCAG GGATGCACTC ACCACCGCCC CGTCCCGCCC CTTCACCTGG
            361 CACCTGGGAG AGCCAGCCAG CTAGGTCGGT GAGGCTGGGG GGCCCAGGAG GTAATGCAGG
            421 GGGTGCTGGG GGTGGTCGTG TTCTTGAGTG TCCCAGCATC CGGATTACCT CCATCTCTCC
            481 CACGCCTGAC CCGCCGACCT CGCTAGAGGA CACATCTGAA ACTTGGGGGG ACGGCTCTCC
            541 TAGAGATTAC CCTCCACCAG AAGGCTTTGG GGGCTATCGA GAGGCTGGAG GTCAGGGCGG
            601 AGGCGCTTTC TTCAGCCCGA GCCCTGGCAG CAGCAGCCTG TCTTCGTGGA GCTTCTTCTC
            661 GGACGCCTCC GACGAGGCAG CCCTGTGAGC GGCCGCACTC CTCAGGTGCA GGCTGCCTAT
            721 CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT
            781 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
            841 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
            901 AGGACATATG GGAGGGCAAA TCATTTAAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT
            961 GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAGGTT GGCTATAAAG AGGTCATCAG
           1021 TATATGAAAC AGCCCCCTGC TGTCCATTCC CTTATTCCAT AGAAAAGCCT GACTGAGGTA
           1081 GATTTTTTTT TATATTTGTT TGTGTATTTT TCTTAACATC CTAATTTCGT ACATGTTTTA
          1141 CTAGCAGAAA TTTTCCCTTC CTCTTCCCTT GA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl