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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Dscr1-pCAGEN

Expression clone of mouse DSCR1.1L

Catalog number RDB12852
Resource name Dscr1-pCAGEN
Alternative name Dscr1.1L/pCAGEN
Clone info. The plasmid expressing mouse DSCR1.1L under the control of the CAG promoter.
Comment Expression was confirmed by the depositor with anti-DSCR1 antibody.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Dscr1 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
Other clones in our bank
External Database
Mouse Dscr1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12852 Dscr1-pCAGEN DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12852_A4Imp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB12852_A4Ima.seq check
          >RDB12852_2_pAxCA_F1_C03_09.ab1
              1 CGAGCCCTGT CAACATGTTA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
             61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA CCATGGAGGA CGGCGTGGCC
            121 GGGCCGCGGC TCGGAGAGGT GGCGGAGGCG GTCGAGGCAC GGGCGCCCCG TAGGGTGACT
            181 CTGCGGCCCT TCGCGCCCTT CTCTGCGGCT GCCGAGGGGG ACGGGGGCGG CGGCGGCGAC
            241 TGGAGCTTCA TCGACTGCGA GATGGAGGAG GTGGATCTGC AGGACCTGCC GAGCGCCACC
            301 ATCGCCTGCC ACCTGGACCC GCGCGTGTTC GTGGACGGCC TGTGCCGGGC CAAATTTGAA
            361 TCCCTCTTCA GAACATATGA CAAGGACACC ACCTTCCAGT ATTTTAAGAG CTTCAAACGT
            421 GTCCGGATAA ACTTCAGCAA CCCCTTATCT GCAGCCGATG CCAGGCTGCG GCTGCACAAG
            481 ACCGAGTTCC TGGGGAAGGA AATGAAGTTG TATTTTGCTC AGACTTTACA CATAGGAAGT
            541 TCACACCTGG CTCCGCCCAA TCCCGACAAA CAGTTCCTCA TCTCCCCTCC GGCCTCTCCT
            601 CCCGTTGGCT GGAAACAAGT AGAAGATGCC ACCCCCGTCA TAAATTACGA TCTTTTATAT
            661 GCCATCTCCA AGCTGGGGCC AGGAGAGAAG TATGAACTGC ATGCAGCGAC AGACACCACT
            721 CCCAGTGTGG TGGTCCACGT GTGTGAGAGT GACCAAGAGA ATGAGGAGGA AGAGGAAGAG
            781 ATGGAGAGAA TGAAGAGACC CAAGCCCAAA ATCATCCAGA CACGGAGACC GGAGTACACA
            841 CCCATCCACC TCAGCTGAGC GGCCGCACTC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG
            901 TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA
            961 AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTTCTGGCTA ATAAGGAAAT
           1021 TTATTTTCAT TGCATAGTGT GTTGGAAATT TTTTGTGTCT CTCACTCGGA GACATATGGG
           1081 AGGCAAATCA ATTAAACATC AGATGAGTAT TGGGTTAGAG TGCCACTATG GCCATATGCT
          1141 GGCTTGCCAT GAACCAAGGT TGGCCTAATT AAG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl