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26/D11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 26/D11
NCBI Gene ID 2538725
Gene Symbol SPCC825.02
Protein Name glucosidase II Gtb1 (predicted)
Synonym
RefSeq Protein NP_588052.1
PomBase(1) SPCC825.02
KEGG(2) spo:SPCC825.02
Depositor-supplied data(3) 26D11
Note 1397A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW010483 SpENT26D11 confirmation required
user_report - reference Olson, L.J., Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition. Biochemistry 54 (26): 4097-4111 (2015). PMID 26062005.
user_report - reference Olson, L.J., Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II, an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum. J. Biol Chem. ;288 (23): 16460-16475 (2013). PMID 23609449.
user_report - reference Stigliano, I.D., Glucosidase II and N-glycan mannose content regulate the half-lives of monoglucosylated species in vivo. Mol. Biol. Cell 22 (11): 1810-1823 (2011). PMID 21471007.
user_report - reference Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557.

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW050483 SpFFH26D11 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW090483 SpYFH26D11 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl