{"id":6420,"date":"2025-07-21T16:04:25","date_gmt":"2025-07-21T07:04:25","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6420"},"modified":"2025-07-21T16:04:33","modified_gmt":"2025-07-21T07:04:33","slug":"gnphumja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/clonedcdnaja\/gnphumja","title":{"rendered":"Genome Network Project Human cDNA Clones"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30af\u30ed\u30fc\u30f3\u306e\u6982\u8981<\/h2>\n<p>\u7406\u7814\uff22\uff32\uff23\u30fb\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u6587\u90e8\u79d1\u5b66\u7701\u300c\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u300d\u304c\u53ce\u96c6\u30fb\u6574\u5099\u3057\u305f\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30af\u30ed\u30fc\u30f3\u306e\u63d0\u4f9b\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u30af\u30ed\u30fc\u30f3\u306f\u3001\u6b21\u306e\uff12\u7a2e\u985e\u3067\u3059\u3002<br \/>\n1)<span style=\"text-decoration: underline;\">\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30b3\u30ec\u30af\u30b7\u30e7\u30f3<\/span><br \/>\n\u6771\u4eac\u5927\u5b66\u306e\u83c5\u91ce\u7d14\u592b\u535a\u58eb\u306a\u3089\u3073\u306b\u7406\u5316\u5b66\u7814\u7a76\u6240\u306e\u6797\u5d0e\u826f\u82f1\u535a\u58eb\u306e\u30b0\u30eb\u30fc\u30d7\u304c\u53ce\u96c6\u30fb\u6574\u5099\u3002\u30d2\u30c8\u306e\u5168\u907a\u4f1d\u5b50\u306e6\u5272\u306b\u76f8\u5f53\u3059\u308b\u304a\u3088\u305d14,000\u907a\u4f1d\u5b50\u3001\u7d043\u4e07\u30af\u30ed\u30fc\u30f3\u3002<br \/>\n2)<span style=\"text-decoration: underline;\">Gateway&reg;\u30a8\u30f3\u30c8\u30ea\u30fc\u30af\u30ed\u30fc\u30f3<\/span><br \/>\n\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u304b\u3089\u629c\u7c8b\u3057\u305f6,300\u907a\u4f1d\u5b50\u5206\u3001\u7d045\u4e07\u30af\u30ed\u30fc\u30f3\u3002Gateway&reg;\u30a8\u30f3\u30c8\u30ea\u30fc\u30af\u30ed\u30fc\u30f3\u306e\u30a4\u30f3\u30b5\u30fc\u30c8DNA\u306f\u3001Gateway&reg;\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306b\u3088\u308a\u7a2e\u3005\u306e\u30c7\u30b9\u30c6\u30a3\u30cd\u30fc\u30b7\u30e7\u30f3\u30d9\u30af\u30bf\u30fc\uff08\u767a\u73fe\u30d9\u30af\u30bf\u30fc\uff09\u3078\u7c21\u5358\u306b\u79fb\u5165\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u306a\u304a\u3001Gateway&reg;\u30a8\u30f3\u30c8\u30ea\u30fc\u30af\u30ed\u30fc\u30f3\u306b\u306f\u3001\u72ec\u7acb\u884c\u653f\u6cd5\u4eba\u65b0\u30a8\u30cd\u30eb\u30ae\u30fc\u30fb\u7523\u696d\u6280\u8853\u7dcf\u5408\u958b\u767a\u7814\u7a76\u6a5f\u69cb\u300c\u5b8c\u5168\u9577cDNA\u69cb\u9020\u89e3\u6790\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u300d\u306b\u304a\u3044\u3066\u3001\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u958b\u767a\u6280\u8853\u7814\u7a76\u7d44\u5408\u304c\u4f5c\u88fd\u3057\u305f\u30d2\u30c8cDNA\u30af\u30ed\u30fc\u30f3\u304b\u3089\u4f5c\u88fd\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3082\u542b\u3093\u3067\u3044\u307e\u3059\u3002<br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/gnp_500.png\" width=\"400\"  \/><\/p>\n<ul>\n<li>\u3053\u308c\u3089\u306e\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30af\u30ed\u30fc\u30f3\u306e\u958b\u767a\u3068\u89e3\u6790\u306e\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u306e\u7814\u7a76\u6210\u679c\u306f\u3001\u4e0b\u8a18\u8ad6\u6587\u3067\u767a\u8868\u3055\u308c\u3001\u30af\u30ed\u30fc\u30f3\u306e\u8a73\u7d30\u60c5\u5831\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u60c5\u5831\u7b49\u304c\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u306e\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9 \u300c<a href=\"https:\/\/cell-innovation.nig.ac.jp\/GNP\/index.html\">\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0<\/a>\u300d\u304b\u3089\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<p>References:<\/p>\n<ol>\n<li>Ota T. et al., Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36: 40-45, 2004.<\/li>\n<li>Otsuki T. et al., Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res., 12: 117-126, 2005.<\/li>\n<li>Kimura K. et al., Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16: 55-65, 2006.<\/li>\n<li>Itoh M., Yasunishi A., Imamura K., Kanamori M., Suzuki H., Suzuki M., Carninci P., Kawai J., Hayashizaki Y. Constructing ORFeome resources with removable termination codons, Biotechniques, 41: 44-48, 2006.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ul>\n<li>\u914d\u5217\u691c\u67fb\u306b\u7528\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u306f<a href=\"#datasheet\">Data Sheet <img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet1.png\" width=\"145\" height=\"30\" \/><\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u6587\u732e\u60c5\u5831\u306f<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07566\">Informaion site <img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet097.gif\" width=\"30\" \/><\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>Gateway&reg;\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306b\u3064\u3044\u3066\u306fLife Technologies\u793e\u306e\u300c<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/gateway-cloning.html\">Gateway \u30af\u30ed\u30fc\u30cb\u30f3\u30b0<\/a>\u300d\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u72ec\u7acb\u884c\u653f\u6cd5\u4eba\u88fd\u54c1\u8a55\u4fa1\u6280\u8853\u57fa\u76e4\u6a5f\u69cb(NITE)\u304c\u63d0\u4f9b\u3057\u3066\u3044\u305f\u30d2\u30c8cDNA\u30af\u30ed\u30fc\u30f3\u306f\u300c\u4e00\u822c\u793e\u56e3\u6cd5\u4eba\u30d0\u30a4\u30aa\u7523\u696d\u60c5\u5831\u5316\u30b3\u30f3\u30bd\u30fc\u30b7\u30a2\u30e0 (JBIC) <a href=\"https:\/\/www.jbic.or.jp\/enterprise_result\/003\">\u30d2\u30c8Gateway(TM)\u30a8\u30f3\u30c8\u30ea\u30fc\u30af\u30ed\u30fc\u30f3\u4e8b\u52d9\u5c40<\/a>\u300d\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u3092\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<h3>\u30af\u30ed\u30fc\u30f3\u306e\u691c\u7d22<\/h3>\n<ul>\n<li>\u30ad\u30fc\u30ef\u30fc\u30c9\u691c\u7d22\u306e\u5834\u5408<br \/>\n<form action=\"https:\/\/knowledge.brc.riken.jp\/resource\/dna\/list\" target=\"_blank\"  accept-charset=\"utf-8\"><input type=\"search\" class=\"field\" name=\"query\" placeholder=\"\u907a\u4f1d\u5b50\u540d\"><input  type=\"hidden\" name=\"__lang__\" value=\"ja\"> <button class=\"button\"  type=\"submit\">Search<\/button><\/form>\n<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"order\"><\/a><\/p>\n<h2>\u4f9d\u983c\u306e\u6982\u8981<\/h2>\n<p>\u25a0\u3000\u5f53\u8a72\u30af\u30ed\u30fc\u30f3\u306f\u5b66\u8853\u6a5f\u95a2\u306b\u3088\u308b\u5b66\u8853\u7814\u7a76\u306b\u9650\u308a\u63d0\u4f9b\u53ef\u80fd\u3067\u3059<\/p>\n<h3>\u4f9d\u983c\u306b\u5fc5\u8981\u306a\u66f8\u5f0f<\/h3>\n<ul>\n<li>\u907a\u4f1d\u5b50\u6750\u6599\u63d0\u4f9b\u4f9d\u983c\u66f8<br \/>\n\n<table id=\"tablepress-13\" class=\"tablepress tablepress-id-13\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmjad\">\u66f8\u5f0fA \uff08\u907a\u4f1d\u5b50\u6750\u6599\u63d0\u4f9b\u4f9d\u983c\u66f8\uff09 [Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmjexord\">\u4f9d\u983c\u66f8\u306e\u8a18\u5165\u4f8b<\/a>]<\/td><td class=\"column-2\">1\u90e8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-13 from cache --><\/p>\n<ul>\n<li>\u300c\u30ab\u30bf\u30ed\u30b0No.\u300d\u3068\u300c\u907a\u4f1d\u5b50\u6750\u6599\u540d\u300d\u306f\u3001\u6b21\u306e\u3088\u3046\u306b\u8a18\u8f09\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30ab\u30bf\u30ed\u30b0No. \u907a\u4f1d\u5b50\u6750\u6599\u540d<br \/>\nHGY088130 IRAL020F10<br \/>\nHGY084224 IRAL010J08<br \/>\nHGE091280 M01C028D08<br \/>\nHGE009243 W01A023B19<\/li>\n<\/ul>\n<\/li>\n<li>\u751f\u7269\u907a\u4f1d\u8cc7\u6e90\u63d0\u4f9b\u540c\u610f\u66f8\u3001MTA<br \/>\n<table>\n<tbody>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/frmjcagnpd\">\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u30af\u30ed\u30fc\u30f3\u5c02\u7528\uff2d\uff34\uff21 \uff08\u975e\u55b6\u5229\u5b66\u8853\u76ee\u7684\u7528\uff09 [Word]<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul>\n<li>\uff2d\uff34\uff21\u306b\u306f\u3001\u500b\u3005\u306e\u30af\u30ed\u30fc\u30f3\u540d\u306e\u8a18\u8f09\u306f\u4e0d\u8981\u3067\u3059<\/li>\n<li>\u5229\u7528\u6761\u4ef6\u306f\uff2d\uff34\uff21\u306b\u3042\u3089\u304b\u3058\u3081\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<li>Gateway&reg;\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306b\u3088\u308a\u4f5c\u88fd\u3055\u308c\u305f\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u3092\u4f7f\u7528\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u300c<a href=\"https:\/\/dna.brc.riken.jp\/ja\/tectradocja\/tec24ceja\">Gateway&reg;\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u4f7f\u7528\u3057\u305f\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u63d0\u4f9b\u6642\u306e\u30e9\u30a4\u30bb\u30f3\u30b9\u6599\u76f8\u5f53\u984d\u6539\u5b9a\u306e\u304a\u77e5\u3089\u305b [link]<\/a>\u300d\u306e\u5185\u5bb9\u3092\u304a\u8aad\u307f\u9802\u304d<a href=\"https:\/\/brc.riken.jp\/dna\/frmegtwpdf\">Limited Use Label License: No.19 (GatewayR Cloning Products) [PDF]<\/a>\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u66f8\u985e\u306e\u9001\u4ed8\u3068\u304a\u554f\u3044\u5408\u308f\u305b<br \/>\n\n<table id=\"tablepress-5\" class=\"tablepress tablepress-id-5\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">\u66f8\u985e\u624b\u7d9a\u304d\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u3001PDF\u3067\u306e\u53d6\u308a\u4ea4\u308f\u3057\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n<ul><br \/>\n\t<li>PDF\u3067\u306e\u53d6\u308a\u4ea4\u308f\u3057\u306e\u53ef\u5426\u306b\u3064\u3044\u3066\u306f\u3001\u4e8b\u524d\u306b\u3054\u6240\u5c5e\u306e\u77e5\u8ca1\u62c5\u5f53\u90e8\u7f72\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30ab\u30e9\u30fc\u30b9\u30ad\u30e3\u30f3\u3057\u305fPDF\u30d5\u30a1\u30a4\u30eb\u3092email\u306b\u3066\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002<\/li><br \/>\n\t<li>\u516c\u5370\u62bc\u5370\u6e08\u307fMTA\u306e\u539f\u672c\uff08\u66f8\u9762\uff09\u306e\u8fd4\u9001\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408\u306f\u3001\u539f\u672c\uff08\u66f8\u9762\uff092\u90e8\u3092\u5f53\u5ba4\u3078\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002\u306a\u304a\u3001\u66f8\u985e\u306e\u8fd4\u9001\u306b\u4fc2\u308b\u9001\u6599\u3092\u3054\u8ca0\u62c5\u3044\u305f\u3060\u304d\u307e\u3059\u3002<\/li><br \/>\n\t<li>\u66f8\u985e\u3092PDF\u3067\u53d6\u308a\u4ea4\u308f\u3059\u5834\u5408\u3067\u3082\u3001\u7de0\u7d50\u6e08\u307f\u66f8\u985e\u306e\u8fd4\u9001\u304c\u5225\u9001\u3068\u306a\u308a\u305d\u308c\u306b\u4fc2\u308b\u9001\u6599\u3092\u3054\u8ca0\u62c5\u3044\u305f\u3060\u304f\u5834\u5408\u304c\u3054\u3056\u3044\u307e\u3059\u3002<br \/>\n\u4f8b\uff09\u3000\u66f8\u985e\u8fd4\u9001\u304c\u5225\u9001\u3068\u306a\u308b\u5834\u5408\u306e\u4f8b<br \/>\n1.\u3000\u51b7\u51cd\u54c1\uff08\u30b2\u30ce\u30e0DNA\uff09\u3084\u7d44\u63db\u3048\u4f53\u306a\u3069\u3001\u30ea\u30bd\u30fc\u30b9\u3092\u5b85\u914d\u4fbf\u3067\u914d\u9001\u3059\u308b\u5834\u5408<br \/>\n2.\u3000\u30ea\u30bd\u30fc\u30b9\u3068\u306f\u5225\u306e\u90e8\u7f72\u3078\u306e\u66f8\u985e\u306e\u8fd4\u9001\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408<br \/>\n3.\u3000\u30ea\u30bd\u30fc\u30b9\u767a\u9001\u524d\u306b\u66f8\u985e\u8fd4\u9001\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408<\/li><br \/>\n<li>\u66f8\u985e\u306e\u9001\u4ed8\u5148\u3001\u554f\u3044\u5408\u308f\u305b\u5148<br \/>\n\n<table id=\"tablepress-1\" class=\"tablepress tablepress-id-1\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">\u3012305-0074\u3000 \u8328\u57ce\u770c\u3064\u304f\u3070\u5e02\u9ad8\u91ce\u53f03-1-1<br \/>\n\u3000 \u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u7814\u7a76\u30bb\u30f3\u30bf\u30fc (BRC)<br \/>\n\u3000 \u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4<br \/>\n\u3000 E-mail: &#100;&#110;&#97;&#98;&#97;&#110;&#107;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<br \/>\n\u3000 TEL: 029-836-3612 (\u5e73\u65e5\u306e9:00\uff5e12:00 \/ 13:00\uff5e17:00)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-1 from cache --><\/li><br \/>\n<li>\u63d0\u4f9b\u7533\u8fbc\u306e\u524d\u306b\u3001\u300c<a href=\"\/ja\/teikyo#step2\">\u7533\u8fbc\u66f8\u985e\u306e\u8a18\u5165\u3068\u9001\u4ed8<\/a>\u300d\u300c<a href=\"\/ja\/teikyo#terms_of_use\">\u3054\u5229\u7528\u306b\u3042\u305f\u3063\u3066<\/a>\u300d\u300c<a href=\"\/ja\/resource\">\u907a\u4f1d\u5b50\u6750\u6599\u306e\u54c1\u8cea\u7ba1\u7406<\/a>\u300d\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li><br \/>\n<li>\u66f8\u5f0f\u306e\u8a73\u7d30\u306f\u300c<a href=\"\/ja\/teikyo\/form\">\u7533\u8fbc\u307f\u306b\u5fc5\u8981\u306a\u66f8\u5f0f<\/a>\u300d\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li><br \/>\n<\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-5 from cache --><\/li>\n<\/ul>\n<h3>\u63d0\u4f9b\u5f62\u614b<\/h3>\n<ul>\n<li>DNA\uff08\u7d041\u03bcg\uff09<\/li>\n<li><span style=\"text-decoration: underline;\">\u5099\u8003<\/span>\uff1a<br \/>\n\u63d0\u4f9b\u524d\u306b\u30d7\u30e9\u30b9\u30df\u30c9\u5168\u9577\u306e\u914d\u5217\u78ba\u8a8d\u3092\u3057\u307e\u3059\u3002<br \/>\n\u767a\u9001\u307e\u3067\uff12\u9031\u9593\u306e\u304a\u6642\u9593\u3092\u9802\u304d\u307e\u3059\u3002\u3054\u4e86\u627f\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<h3>\u63d0\u4f9b\u624b\u6570\u6599<\/h3>\n<ul>\n<li><span style=\"text-decoration: underline;\">\u63d0\u4f9b\u624b\u6570\u6599<\/span>\uff1a\uff081\u672c\u3042\u305f\u308a)<br \/>\n<strong>\n<table id=\"tablepress-9\" class=\"tablepress tablepress-id-9\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><ul><li>\u300c\u63d0\u4f9b\u624b\u6570\u6599\u300d\u3068\u300c\u975e\u55b6\u5229\u5b66\u8853\u76ee\u7684\u5229\u7528\u300d\u304a\u3088\u3073\u300c\u55b6\u5229\u76ee\u7684\u5229\u7528\u300d\u306e\u533a\u5206\u306f\u3001\u300c<a href=\"https:\/\/brc.riken.jp\/dna\/frmfeej\">\u63d0\u4f9b\u624b\u6570\u6599\u3068\u8acb\u6c42<\/a>\u300d\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li><li>\u5225\u9014\u3001\u30ea\u30bd\u30fc\u30b9\u306e\u9001\u6599\u4e26\u3073\u306b\u7bb1\u4ee3\u53ca\u3073\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u4ee3\u7b49\u304c\u52a0\u7b97\u3055\u308c\u307e\u3059\u3002<\/li><li>\u8acb\u6c42\u66f8\u306f\u63d0\u4f9b\u4f9d\u983c\u66f8\u306b\u8a18\u8f09\u3055\u308c\u305f\u300c\u62c5\u5f53\u8005\u300d\u306b\u9001\u4ed8\u3044\u305f\u3057\u307e\u3059\u3002\u7279\u5225\u306a\u69d8\u5f0f\u306e\u8acb\u6c42\u66f8\u30fb\u7d0d\u54c1\u66f8\u30fb\u9818\u53ce\u66f8\u3092\u5fc5\u8981\u3068\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u4e88\u3081\u305d\u306e\u65e8\u3092\u3054\u76f8\u8ac7\u3044\u305f\u3060\u304f\u3088\u3046\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002\u8acb\u6c42\u66f8\u306f\u907a\u4f1d\u5b50\u6750\u6599\u3068\u306f\u5225\u306b\u9001\u4ed8\u3044\u305f\u3057\u307e\u3059\u3002<\/li><li>\u63d0\u4f9b\u624b\u6570\u6599\u306f\u6307\u5b9a\u306e\u9280\u884c\u53e3\u5ea7\u306b\u304a\u632f\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002\u632f\u8fbc\u307f\u624b\u6570\u6599\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u3001\u4f9d\u983c\u8005\u8ca0\u62c5\u306b\u3066\u304a\u9858\u3044\u81f4\u3057\u307e\u3059\u3002<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-9 from cache --><\/strong><\/li>\n<li>\u30d9\u30af\u30bf\u30fc\u304cpCMV SPORT6\u30b7\u30ea\u30fc\u30ba(Gateway&reg;\u767a\u73fe\u30d9\u30af\u30bf\u30fc)\u306e\u5834\u5408\u3001\u30e9\u30a4\u30bb\u30f3\u30b9\u6599\u76f8\u5f53\u984d\u3092\u8ffd\u52a0\u3067\u3054\u8ca0\u62c5\u3044\u305f\u3060\u304d\u307e\u3059\u3002\u30e9\u30a4\u30bb\u30f3\u30b9\u306b\u3064\u3044\u3066\u306f\u300c<a href=\"https:\/\/dna.brc.riken.jp\/ja\/tectradocja\/tec24ceja\">Gateway&reg;\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u4f7f\u7528\u3057\u305f\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u63d0\u4f9b\u6642\u306e\u30e9\u30a4\u30bb\u30f3\u30b9\u6599\u76f8\u5f53\u984d\u6539\u5b9a\u306e\u304a\u77e5\u3089\u305b [link]<\/a>\u300d\u3092\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<hr \/>\n<h3>\u5229\u7528\u6761\u4ef6<\/h3>\n<ol>\n<li>\u5229\u7528\u8005\u306f\u3001\u7814\u7a76\u6210\u679c\u306e\u516c\u8868\u306b\u3042\u305f\u3063\u3066\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u958b\u767a\u6280\u8853\u7814\u7a76\u7d44\u5408\uff08\u4ee5\u4e0b\u300c\u30d0\u30a4\u30aa\u7d44\u5408\u300d\u3068\u3044\u3046\u3002\uff09\u3001\u7406\u5316\u5b66\u7814\u7a76\u6240 \u6797\u5d0e \u826f\u82f1\u535a\u58eb(\u6797\u5d0e\u535a\u58eb)\u4e26\u3073\u306b\u6771\u4eac\u5927\u5b66\u3000\u83c5\u91ce\u7d14\u592b\u6559\u6388(\u83c5\u91ce\u6559\u6388)\u306e\u6307\u5b9a\u3059\u308b\u6587\u732e\u3092\u5f15\u7528\u3059\u308b\u3002\n<ul>\n<li>Ota T. et al., Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36: 40-45, 2004.<\/li>\n<li>Otsuki T. et al., Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res., 12: 117-126, 2005.<\/li>\n<li>Kimura K. et al., Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes. Genome Res., 16: 55-65, 2006.<\/li>\n<li>Itoh M., Yasunishi A., Imamura K., Kanamori M., Suzuki H., Suzuki M., Carninci P., Kawai J., Hayashizaki Y. Constructing ORFeome resources with removable termination codons, Biotechniques, 41: 44-48, 2006.<\/li>\n<\/ul>\n<\/li>\n<li>\u5229\u7528\u8005\u306f\u3001\u7814\u7a76\u6210\u679c\u306e\u516c\u8868\u306b\u3042\u305f\u3063\u3066\u30d0\u30a4\u30aa\u7d44\u5408\u3001\u6797\u5d0e\u535a\u58eb\u4e26\u3073\u306b\u83c5\u91ce\u6559\u6388\u3078\u306e\u8b1d\u8f9e\u306e\u8868\u660e\u3092\u5fc5\u8981\u3068\u3059\u308b\u3002<\/li>\n<li>\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u306e\u4f7f\u7528\u306f\u5b66\u8853\u6a5f\u95a2\u306b\u3088\u308b\u5b66\u8853\u7814\u7a76\u306b\u9650\u308b\u3002<\/li>\n<li>\u30d0\u30a4\u30aa\u7d44\u5408\u306f\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u306e\u4f7f\u7528\u306b\u3088\u308b\u6f5c\u5728\u7684\u306a\u7b2c\u4e09\u8005\u306e\u7279\u8a31\u3001\u8457\u4f5c\u6a29\u3001\u5546\u6a19\u3082\u3057\u304f\u306f\u305d\u306e\u4ed6\u306e\u6240\u6709\u6a29\u7b49\u306e\u6a29\u5229\u4fb5\u5bb3\u7b49\u306b\u3064\u3044\u3066\u3044\u304b\u306a\u308b\u4fdd\u8a3c\u3092\u3059\u308b\u3082\u306e\u3067\u306f\u306a\u3044\u3002\u5229\u7528\u8005\u306f\u3001\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u306e\u4f7f\u7528\u3001\u4fdd\u5b58\u3001\u51e6\u5206\u7b49\u306b\u3088\u3063\u3066\u751f\u3058\u308b\u3044\u304b\u306a\u308b\u640d\u5bb3\u53ca\u3073\u7b2c\u4e09\u8005\u304b\u3089\u306e\u640d\u5bb3\u8ce0\u511f\u7b49\u306e\u8acb\u6c42\u306b\u3064\u3044\u3066\u5168\u3066\u306e\u8cac\u4efb\u3092\u8ca0\u3046\u3082\u306e\u3068\u3059\u308b\u3002<\/li>\n<\/ol>\n<h3>\u304a\u554f\u3044\u5408\u305b\u5148<\/h3>\n<p><strong>\n<table id=\"tablepress-1\" class=\"tablepress tablepress-id-1\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">\u3012305-0074\u3000 \u8328\u57ce\u770c\u3064\u304f\u3070\u5e02\u9ad8\u91ce\u53f03-1-1<br \/>\n\u3000 \u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u7814\u7a76\u30bb\u30f3\u30bf\u30fc (BRC)<br \/>\n\u3000 \u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4<br \/>\n\u3000 E-mail: &#100;&#110;&#97;&#98;&#97;&#110;&#107;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<br \/>\n\u3000 TEL: 029-836-3612 (\u5e73\u65e5\u306e9:00\uff5e12:00 \/ 13:00\uff5e17:00)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-1 from cache --><\/strong><\/p>\n<hr \/>\n<p><a name=\"datasheet\"><\/a><br \/>\n<img loading=\"lazy\" decoding=\"async\" alt=\"data sheet\" src=\"https:\/\/dnaconda.riken.jp\/images\/datasheet1.png\" width=\"145\" height=\"30\" \/><\/p>\n<h3>Primers for sequencing vectors<\/h3>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Vectors<\/th>\n<th>Primers<\/th>\n<\/tr>\n<tr valign=\"Top\">\n<td>pBluescriptR<\/td>\n<td>Insert 5&#8242;: T7 (Pr0202) GTAATACGACTCACTATAGGGC<br \/>\nInsert 3&#8242;: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pCMV-SPORT6<\/td>\n<td>Insert 5&#8242;: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG<br \/>\nInsert 3&#8242;: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA<br \/>\nUpstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN<br \/>\n(V: mixture of A,G and C)<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pCMV-SPORT6.1<\/td>\n<td>Insert 5&#8242;: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG<br \/>\nInsert 3&#8242;: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA<br \/>\nUpstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN<br \/>\n(V: mixture of A,G and C)<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pCMV-SPORT6.ccdb<\/td>\n<td>Insert 5&#8242;: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG<br \/>\nInsert 3&#8242;: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA<br \/>\nUpstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN<br \/>\n(V: mixture of A,G and C)<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pCR4-TOPO<\/td>\n<td>Insert 5&#8242;: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA<br \/>\nInsert 3&#8242;: Reverse2 (Pr0100) GCGGATAACAATTTCACACAGG<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pDONR221<\/td>\n<td>Insert 5&#8242;: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA<br \/>\nInsert 3&#8242;:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pDNR-Dual<\/td>\n<td>Insert 5&#8242;:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG<br \/>\nInsert 3&#8242;:Dual-R (Pr0072) ATCCAAGCACTAGTCATGAC<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pDNR-LIB<\/td>\n<td>Insert 5&#8242;:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG<br \/>\nInsert 3&#8242;: M13 Reverse (Pr0042) AAACAGCTATGACCATGTTCA<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pENTR\/D-TOPO<\/td>\n<td>Insert 5&#8242;: M13_-40 (Pr0410) GTTTTCCCAGTCACGACGTTGTA<br \/>\nInsert 3&#8242;:T7long (Pr0244) CGCCAAGCTCTAATACGACTCACTATAGGG<\/td>\n<\/tr>\n<tr valign=\"Top\">\n<td>pOTB7 (pBR322 ori)<\/td>\n<td>Insert 5&#8242;: pOTB7_F (Pr0224) AACGCGGCTACAATTAATACATAACC<br \/>\nInsert 3&#8242;: pOTB7_R (Pr0225) GTACTGCAGCCGATTCATTAATGC<br \/>\nUpstream of poly(A): TTTTTTTTTTTTTTTTTTTTVNN<br \/>\n(V: mixture of A,G and C)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p style=\"text-align:left;\">2025.07.21 (T.M. GRP0032j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30af\u30ed\u30fc\u30f3\u306e\u6982\u8981 \u7406\u7814\uff22\uff32\uff23\u30fb\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u6587\u90e8\u79d1\u5b66\u7701\u300c\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u300d\u304c\u53ce\u96c6\u30fb\u6574\u5099\u3057\u305f\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30af\u30ed\u30fc\u30f3\u306e\u63d0\u4f9b\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u30af\u30ed\u30fc\u30f3\u306f\u3001\u6b21\u306e\uff12\u7a2e\u985e\u3067\u3059\u3002 1)\u30d2\u30c8\u5b8c\u5168\u9577cDNA\u30b3\u30ec\u30af\u30b7\u30e7\u30f3 \u6771\u4eac\u5927\u5b66\u306e\u83c5\u91ce\u7d14\u592b\u535a\u58eb\u306a\u3089\u3073\u306b\u7406\u5316\u5b66\u7814\u7a76\u6240\u306e\u6797\u5d0e\u826f\u82f1\u535a\u58eb\u306e\u30b0\u30eb\u30fc\u30d7\u304c\u53ce\u96c6\u30fb\u6574\u5099\u3002\u30d2\u30c8\u306e\u5168\u907a\u4f1d\u5b50\u306e6\u5272\u306b\u76f8\u5f53\u3059\u308b\u304a\u3088\u305d14,000 [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":6385,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6420","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6420","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6420"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6420\/revisions"}],"predecessor-version":[{"id":6424,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6420\/revisions\/6424"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6385"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}