{"id":6322,"date":"2025-07-21T14:44:45","date_gmt":"2025-07-21T05:44:45","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6322"},"modified":"2025-07-21T14:45:01","modified_gmt":"2025-07-21T05:45:01","slug":"thermoja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/researchja\/thermoja","title":{"rendered":"Thermophile Gene Expression Plasmid"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><br \/>\n<i>Aeropyrum pernix<\/i>, <i>Pyrococcus horikoshii<\/i>, <i>Sulfolobus tokodaii<\/i> and <i>Thermus thermophilus<\/i> gene expression plasmids and <i>T thermophilus<\/i> gene disruption plasmids were deposited by Dr.Yutaka Kawarabayasi, National Institute of Advanced Industrial Science and Dr. Seiki Kuramitsu, Osaka University. <\/p>\n<h3>\u63d0\u4f9b\u30ea\u30bd\u30fc\u30b9\u30ea\u30b9\u30c8<\/h3>\n<ul>\n<li>\u5927\u8178\u83cc\u3067\u597d\u71b1\u83cc\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3059\u308b\u305f\u3081\u306e\u30d7\u30e9\u30b9\u30df\u30c9\n<ul>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_ape\"><i>Aeropyrum pernix<\/i><\/a><\/li>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_pho\"><i>Pyrococcus horikoshii<\/i><\/a><\/li>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_sto\"><i>Sulfolobus tokodaii<\/i><\/a><\/li>\n<li><a href=\"\/en\/researchen\/thermoen\/thermo_tth\"><i>Thermus thermophilus<\/i><\/a><\/li>\n<\/ul>\n<\/li>\n<li><i>Thermus thermophilus<\/i> \u907a\u4f1d\u5b50\u306e<a href=\"\/en\/researchen\/thermoen\/thermo_tth\">\u7834\u58ca\u682a\u4f5c\u88fd\u7528\u30d7\u30e9\u30b9\u30df\u30c9<\/a><\/li>\n<li>\u30d7\u30e9\u30b9\u30df\u30c9\u306fDNA\u6eb6\u6db2 (TE buffer)\u3067\u304a\u9001\u308a\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n<hr \/>\n<h3>\u5165\u624b\u65b9\u6cd5<\/h3>\n<h4>\u66f8\u985e\u306e\u5165\u624b\u3068\u8a18\u5165<\/h4>\n<ul>\n<li>\u751f\u7269\u907a\u4f1d\u8cc7\u6e90\u63d0\u4f9b\u4f9d\u983c\u66f8\n<ol>\n<li>\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\u3068\u30d7\u30e9\u30b9\u30df\u30c9\u756a\u53f7 (\u4f8b\uff1aTHR041204, StoEx03A04)\u3092\u8a18\u5165<\/li>\n<\/ol>\n<p>\n<table id=\"tablepress-13\" class=\"tablepress tablepress-id-13\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmjad\">\u66f8\u5f0fA \uff08\u907a\u4f1d\u5b50\u6750\u6599\u63d0\u4f9b\u4f9d\u983c\u66f8\uff09 [Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmjexord\">\u4f9d\u983c\u66f8\u306e\u8a18\u5165\u4f8b<\/a>]<\/td><td class=\"column-2\">1\u90e8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-13 from cache -->\n<\/li>\n<li>\u751f\u7269\u907a\u4f1d\u8cc7\u6e90\u63d0\u4f9b\u540c\u610f\u66f8\n<ol>\n<li>\u7531\u6765\u597d\u71b1\u83cc\u3054\u3068\u306b\u4f5c\u6210\u3057\u3001\u30ea\u30bd\u30fc\u30b9\u306f\u300cAeropyrum pernix\u907a\u4f1d\u5b50\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\uff08\u5225\u6dfb\u53c2\u7167\uff09\u300d\u3001\u300cPyrococcus horikoshii\u907a\u4f1d\u5b50\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\uff08\u5225\u6dfb\u53c2\u7167\uff09\u300d\u3042\u308b\u3044\u306f\u300cSulfolobus tokodaii\u907a\u4f1d\u5b50\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\uff08\u5225\u6dfb\u53c2\u7167\uff09\u300d\u3068\u8a18\u5165<\/li>\n<li>\u56fa\u6709\u8a18\u53f7\u6b04\u306e\u8a18\u5165\u306f\u4e0d\u8981<\/li>\n<li>\u30ea\u30bd\u30fc\u30b9\u306e\u5229\u7528\u6761\u4ef6\u6b04\u306b\u306f\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u6bce\u306b\u30a6\u30a7\u30d6\u30ab\u30bf\u30ed\u30b0\u3092\u3054\u53c2\u7167\u3044\u305f\u3060\u304d\u3001\u8a18\u5165\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059<\/li>\n<li>\u5229\u7528\u76ee\u7684\u306b\u3088\u308a\u3001\u975e\u55b6\u5229\u5b66\u8853\u76ee\u7684\u7528MTA\u3042\u308b\u3044\u306f\u55b6\u5229\u76ee\u7684\u7528MTA\u3092\u9078\u629e\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ol>\n<p>\n<table id=\"tablepress-15\" class=\"tablepress tablepress-id-15\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><a href=\"\/DataSheet\/frmjcad\">\u66f8\u5f0fC (\u751f\u7269\u907a\u4f1d\u8cc7\u6e90\u63d0\u4f9b\u540c\u610f\u66f8, MTA) \u975e\u55b6\u5229\u5b66\u8853\u76ee\u7684\u7528&nbsp;[Word]<\/a><br \/><br \/>\n<a href=\"\/DataSheet\/frmjcpd\">\u66f8\u5f0fC (\u751f\u7269\u907a\u4f1d\u8cc7\u6e90\u63d0\u4f9b\u540c\u610f\u66f8, MTA) \u55b6\u5229\u76ee\u7684\u7528&nbsp;[Word]<\/a><\/td><td class=\"column-2\">2\u90e8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-15 from cache -->\n<\/li>\n<\/ul>\n<p>&nbsp;<br \/>\n\u66f8\u985e\u306e\u9001\u4ed8\u3068\u304a\u554f\u3044\u5408\u308f\u305b<br \/>\n\n<table id=\"tablepress-5\" class=\"tablepress tablepress-id-5\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">\u66f8\u985e\u624b\u7d9a\u304d\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u3001PDF\u3067\u306e\u53d6\u308a\u4ea4\u308f\u3057\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n<ul><br \/>\n\t<li>PDF\u3067\u306e\u53d6\u308a\u4ea4\u308f\u3057\u306e\u53ef\u5426\u306b\u3064\u3044\u3066\u306f\u3001\u4e8b\u524d\u306b\u3054\u6240\u5c5e\u306e\u77e5\u8ca1\u62c5\u5f53\u90e8\u7f72\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30ab\u30e9\u30fc\u30b9\u30ad\u30e3\u30f3\u3057\u305fPDF\u30d5\u30a1\u30a4\u30eb\u3092email\u306b\u3066\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002<\/li><br \/>\n\t<li>\u516c\u5370\u62bc\u5370\u6e08\u307fMTA\u306e\u539f\u672c\uff08\u66f8\u9762\uff09\u306e\u8fd4\u9001\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408\u306f\u3001\u539f\u672c\uff08\u66f8\u9762\uff092\u90e8\u3092\u5f53\u5ba4\u3078\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002\u306a\u304a\u3001\u66f8\u985e\u306e\u8fd4\u9001\u306b\u4fc2\u308b\u9001\u6599\u3092\u3054\u8ca0\u62c5\u3044\u305f\u3060\u304d\u307e\u3059\u3002<\/li><br \/>\n\t<li>\u66f8\u985e\u3092PDF\u3067\u53d6\u308a\u4ea4\u308f\u3059\u5834\u5408\u3067\u3082\u3001\u7de0\u7d50\u6e08\u307f\u66f8\u985e\u306e\u8fd4\u9001\u304c\u5225\u9001\u3068\u306a\u308a\u305d\u308c\u306b\u4fc2\u308b\u9001\u6599\u3092\u3054\u8ca0\u62c5\u3044\u305f\u3060\u304f\u5834\u5408\u304c\u3054\u3056\u3044\u307e\u3059\u3002<br \/>\n\u4f8b\uff09\u3000\u66f8\u985e\u8fd4\u9001\u304c\u5225\u9001\u3068\u306a\u308b\u5834\u5408\u306e\u4f8b<br \/>\n1.\u3000\u51b7\u51cd\u54c1\uff08\u30b2\u30ce\u30e0DNA\uff09\u3084\u7d44\u63db\u3048\u4f53\u306a\u3069\u3001\u30ea\u30bd\u30fc\u30b9\u3092\u5b85\u914d\u4fbf\u3067\u914d\u9001\u3059\u308b\u5834\u5408<br \/>\n2.\u3000\u30ea\u30bd\u30fc\u30b9\u3068\u306f\u5225\u306e\u90e8\u7f72\u3078\u306e\u66f8\u985e\u306e\u8fd4\u9001\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408<br \/>\n3.\u3000\u30ea\u30bd\u30fc\u30b9\u767a\u9001\u524d\u306b\u66f8\u985e\u8fd4\u9001\u3092\u3054\u5e0c\u671b\u306e\u5834\u5408<\/li><br \/>\n<li>\u66f8\u985e\u306e\u9001\u4ed8\u5148\u3001\u554f\u3044\u5408\u308f\u305b\u5148<br \/>\n\n<table id=\"tablepress-1\" class=\"tablepress tablepress-id-1\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">\u3012305-0074\u3000 \u8328\u57ce\u770c\u3064\u304f\u3070\u5e02\u9ad8\u91ce\u53f03-1-1<br \/>\n\u3000 \u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u7814\u7a76\u30bb\u30f3\u30bf\u30fc (BRC)<br \/>\n\u3000 \u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4<br \/>\n\u3000 E-mail: &#100;&#110;&#97;&#98;&#97;&#110;&#107;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<br \/>\n\u3000 TEL: 029-836-3612 (\u5e73\u65e5\u306e9:00\uff5e12:00 \/ 13:00\uff5e17:00)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li><br \/>\n<li>\u63d0\u4f9b\u7533\u8fbc\u306e\u524d\u306b\u3001\u300c<a href=\"\/ja\/teikyo#step2\">\u7533\u8fbc\u66f8\u985e\u306e\u8a18\u5165\u3068\u9001\u4ed8<\/a>\u300d\u300c<a href=\"\/ja\/teikyo#terms_of_use\">\u3054\u5229\u7528\u306b\u3042\u305f\u3063\u3066<\/a>\u300d\u300c<a href=\"\/ja\/resource\">\u907a\u4f1d\u5b50\u6750\u6599\u306e\u54c1\u8cea\u7ba1\u7406<\/a>\u300d\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li><br \/>\n<li>\u66f8\u5f0f\u306e\u8a73\u7d30\u306f\u300c<a href=\"\/ja\/teikyo\/form\">\u7533\u8fbc\u307f\u306b\u5fc5\u8981\u306a\u66f8\u5f0f<\/a>\u300d\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li><br \/>\n<\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-5 from cache --><br \/>\n<span style=\"text-decoration: underline;\">\u63d0\u4f9b\u624b\u6570\u6599<\/span>\uff1a\uff081\u672c\u3042\u305f\u308a)<br \/>\n\n<table id=\"tablepress-10\" class=\"tablepress tablepress-id-10\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><ul><li>\u63d0\u4f9b\u624b\u6570\u6599\u306f\u975e\u55b6\u5229\u5b66\u8853\u76ee\u7684\u5229\u7528\u3068\u55b6\u5229\u76ee\u7684\u5229\u7528\u3067\u7570\u306a\u308a\u307e\u3059\u3002\u300c\u63d0\u4f9b\u624b\u6570\u6599\u300d\u3068\u300c\u975e\u55b6\u5229\u5b66\u8853\u76ee\u7684\u5229\u7528\u300d\u304a\u3088\u3073\u300c\u55b6\u5229\u76ee\u7684\u5229\u7528\u300d\u306e\u533a\u5206\u306f\u3001\u300c<a href=\"https:\/\/brc.riken.jp\/dna\/frmfeej\">\u63d0\u4f9b\u624b\u6570\u6599\u3068\u8acb\u6c42<\/a>\u300d\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li><li>\u5225\u9014\u3001\u30ea\u30bd\u30fc\u30b9\u306e\u9001\u6599\u4e26\u3073\u306b\u7bb1\u4ee3\u53ca\u3073\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u4ee3\u7b49\u304c\u52a0\u7b97\u3055\u308c\u307e\u3059\u3002<\/li><li>\u8acb\u6c42\u66f8\u306f\u63d0\u4f9b\u4f9d\u983c\u66f8\u306b\u8a18\u8f09\u3055\u308c\u305f\u300c\u62c5\u5f53\u8005\u300d\u306b\u9001\u4ed8\u3044\u305f\u3057\u307e\u3059\u3002\u7279\u5225\u306a\u69d8\u5f0f\u306e\u8acb\u6c42\u66f8\u30fb\u7d0d\u54c1\u66f8\u30fb\u9818\u53ce\u66f8\u3092\u5fc5\u8981\u3068\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u4e88\u3081\u305d\u306e\u65e8\u3092\u3054\u76f8\u8ac7\u3044\u305f\u3060\u304f\u3088\u3046\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002\u8acb\u6c42\u66f8\u306f\u907a\u4f1d\u5b50\u6750\u6599\u3068\u306f\u5225\u306b\u9001\u4ed8\u3044\u305f\u3057\u307e\u3059\u3002<\/li><li>\u63d0\u4f9b\u624b\u6570\u6599\u306f\u6307\u5b9a\u306e\u9280\u884c\u53e3\u5ea7\u306b\u304a\u632f\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002\u632f\u8fbc\u307f\u624b\u6570\u6599\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u3001\u4f9d\u983c\u8005\u8ca0\u62c5\u306b\u3066\u304a\u9858\u3044\u81f4\u3057\u307e\u3059\u3002<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-10 from cache --><\/p>\n<hr \/>\n<p style=\"text-align: left;\">2025.07.21 (T.M. GRP0062j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Aeropyrum pernix, Pyrococcus horikoshii, Sulfolobus tokodaii and Thermus thermophilus gene expression plasmids and T thermophilus gene disruption plasmids were deposited by Dr.Yutaka Kawarabayasi, Nat [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":5842,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6322","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6322","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6322"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6322\/revisions"}],"predecessor-version":[{"id":6326,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6322\/revisions\/6326"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5842"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6322"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}