{"id":6275,"date":"2025-07-21T14:08:41","date_gmt":"2025-07-21T05:08:41","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6275"},"modified":"2025-07-21T14:09:02","modified_gmt":"2025-07-21T05:09:02","slug":"luciferaseja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/luminescenceja\/luciferaseja","title":{"rendered":"\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>\u9ad8\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/h2>\n<p><a name=\"a\"><\/a><\/p>\n<h3>Akaluc \u65b0\u898f\u958b\u767a\u306e\u8d64\u8272\u767a\u5149\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/h3>\n<p>\u30db\u30bf\u30eb\u306e\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u751f\u547d\u73fe\u8c61\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u767a\u5149\u57fa\u8cea\u306e\u7d44\u7e54\u900f\u904e\u6027\u304c\u4f4e\u3044\u3068\u3044\u3046\u6b20\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u7406\u7814\u8133\u795e\u7d4c\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u5bae\u8107 \u6566\u53f2\u5148\u751f\u3001\u5ca9\u91ce \u667a\u5148\u751f\u3001\u96fb\u6c17\u901a\u4fe1\u5927\u5b66\u5927\u5b66\u306e\u7267 \u660c\u6b21\u90ce\u5148\u751f\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u751f\u304d\u305f\u52d5\u7269\u500b\u4f53\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u4eba\u5de5\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0AkaBLI\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>AkaBLI\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u7d44\u7e54\u900f\u904e\u6027\u306e\u5411\u4e0a\u3057\u305f\u4eba\u5de5\u57fa\u8ceaAkaLumine\u3068AkaLumine\u306b\u5408\u308f\u305b\u3066\u958b\u767a\u3057\u305f\u4eba\u5de5\u9175\u7d20Akaluc\u3067\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002AkaBLI\u306e\u767a\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5f37\u5ea6\u306f\u3001\u5f93\u6765\u3068\u6bd4\u3079100\uff5e1000\u500d\u3067\u3059\u3002<\/p>\n<p>\u5bae\u8107\u7814\u7a76\u5ba4\u3088\u308a\u3001\u4eba\u5de5\u9175\u7d20Akaluc\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u304c\u5f53\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u306b\u5bc4\u8a17\u3055\u308c\u3001\u63d0\u4f9b\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n<ul style=\"line-height: 2em;\">\n<li>\u8ad6\u6587<br \/>\nNakashiba, T. et al., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038\/s41684-023-01238-6. Epub ahead of print. PMID 37679611.<br \/>\nIwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.<\/li>\n<li>\u30d7\u30ec\u30b9<br \/>\n<a href=\"https:\/\/web.brc.riken.jp\/ja\/archives\/news\/20230908_01\">\u300c\u751f\u4f53\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u591a\u8272\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u7528\u30de\u30a6\u30b9\u300d<\/a> (2023\/9\/8 \u7406\u7814BRC)<br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2018\/20180223_1\/\">\u300c\u8133\u306e\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u4eba\u5de5\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0AkaBLI\u300d<\/a> (2018\/2\/23 \u7406\u7814)<\/li>\n<li>DNA resource<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15781\">pcDNA3 Venus-Akaluc (cat# RDB15781)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15782\">pAAV2 SynTetOff Venus-Akaluc (cat# RDB15782)<\/a><script>newImage(2021,03,28)<\/script><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15783\">pAAV2 TRE Venus-Akaluc (cat# RDB15783)<\/a><script>newImage(2021,03,28)<\/script><\/li>\n<\/ul>\n<p style=\"text-align: right;\">(2018.05.07 N.N.)<\/p>\n<hr \/>\n<p><a name=\"l\"><\/a><\/p>\n<h3>D-\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u306e\u307e\u307e\u9ad8\u3044\u5f37\u5ea6\u306e\u767a\u5149\u304c\u5f97\u3089\u308c\u308b\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc<\/h3>\n<p>\u4e00\u822c\u7684\u306a\u5317\u7c73\u7523\u30db\u30bf\u30eb\u7531\u6765\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3068\u6bd4\u8f03\u3057\u3066\u30012 \u500d\u4ee5\u4e0a\u3001\u6700\u5927\u3067 \uff14\u500d\u7a0b\u306e\u660e\u308b\u3044\u767a\u5149\u5f37\u5ea6\u304c\u5f97\u3089\u308c\u308b\u65e5\u672c\u7523\u3001\u306a\u3089\u3073\u306b\u30de\u30ec\u30fc\u30b7\u30a2\u7523 ( \u203b1 ) \u30db\u30bf\u30eb\u7531\u6765\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\u3067\u3059\u3002\u30aa\u30ea\u30f3\u30d1\u30b9\u682a\u5f0f\u4f1a\u793e \u5c0f\u6c5f\u514b\u5178\u5148\u751f\u3089\u306b\u3088\u3063\u3066\u958b\u767a\u3055\u308c\u307e\u3057\u305f\u3002\u57fa\u8cea\u3067\u3042\u308b D- \u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u3068\u306e\u53cd\u5fdc\u306b\u3088\u3063\u3066\u9ad8\u3044\u767a\u5149\u5f37\u5ea6\u3092\u5f97\u3089\u308c\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u53c2\u8003\u6587\u732e 1\u30012\u30013\u30014\u30015\uff09\u3002<\/p>\n<p>1. \u30db\u30bf\u30eb\u7531\u6765\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3000\u7279\u958b2012-235756<br \/>\n2. \u30db\u30bf\u30eb\u7531\u6765\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3000\u7279\u958b2013-74861<br \/>\n3. \u30db\u30bf\u30eb\u7531\u6765\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3000\u7279\u958b2013-138668<br \/>\n4. \u30db\u30bf\u30eb\u7531\u6765\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3000\u7279\u958b2013-81459<br \/>\n5. FIREFLY LUCIFERASE United States Patent Application US 2015\/0291938 A1<\/p>\n<ul style=\"line-height: 2em;\">\n<li>\u8ad6\u6587<br \/>\nNakashiba, T. et al., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY). 2023 Sep 7. doi: 10.1038\/s41684-023-01238-6. Epub ahead of print. PMID 37679611.<\/li>\n<li>\u30d7\u30ec\u30b9<br \/>\n<a href=\"https:\/\/web.brc.riken.jp\/ja\/archives\/news\/20230908_01\">\u300c\u751f\u4f53\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u591a\u8272\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u7528\u30de\u30a6\u30b9\u300d<\/a> (2023\/9\/8 \u7406\u7814BRC)<script>newImage(2023,09,13)<\/script><\/li>\n<\/ul>\n<table>\n<tbody>\n<tr>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_001.png\" width=\"300\">\n<\/td>\n<td valign=\"top\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_002.png\" width=\"300\">\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" valign=\"top\">\n\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092HeLa\u7d30\u80de\u306b\u5c0e\u5165\u3057\u30012mM D-luciferin\u5b58\u5728\u4e0b\u3067\u767a\u5149\u3055\u305b\u305f\u3002\u5de6\u4e0a\u304b\u3089PmatLuc1\u3001DkumLuc1\u3001MALuci2Luc\u3002\u53f3\u4e0a\u304b\u3089SflaRE1Luc\u3001PsagRE1Luc\u3002\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>\u9078\u3079\u308b 3 \u8272\u767a\u5149 \uff01\u591a\u8272\u89b3\u5bdf\u306b\u5fdc\u7528\u3067\u304d\u307e\u3059<\/h3>\n<p>D- \u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u3092\u57fa\u8cea\u3068\u3057\u3066<span style=\"color: #008000;\">\u7dd1\u8272<\/span>\u3001<span style=\"color: #d0d000;\">\u9ec4\u8272<\/span>\u3001<span style=\"color: #ee0020;\">\u8d64\u8272<\/span>\u306e\u767a\u5149\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u308c\u3089\u3092\u7d44\u307f\u5408\u308f\u305b\u305f\u591a\u8272\u767a\u5149\u89b3\u5bdf\u306b\u3082\u5fdc\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<table width=\"100%\" border=\"1\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th rowspan=\"2\">Catalog no.<\/th>\n<th rowspan=\"2\">Name of clone<\/th>\n<th rowspan=\"2\">Origin<\/th>\n<th rowspan=\"2\">Intracellular luminescence intensity(vie, Phothinus pyralis)<\/th>\n<th colspan=\"2\">Maximal absorption wavelength (pH8, in vitro)<\/th>\n<th rowspan=\"2\">misc.<\/th>\n<\/tr>\n<tr>\n<th>25\u2103<\/th>\n<th>37\u2103<\/th>\n<tr>\n<td>RDB14359<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14359\">pPmatLuc1<\/a><\/td>\n<td>Pyrocoelia matsumurai<\/a><\/td>\n<td>4 times or more<\/td>\n<td>560 nm<\/td>\n<td>567 nm<\/td>\n<td rowspan=\"5\">luciferase cDNA is cloned in pUC19 vector.<\/td>\n<\/tr>\n<tr>\n<td>RDB14360<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14360\">pDkumLuc1<\/a><\/td>\n<td>Drilaster kumejimensis<\/a><\/td>\n<td>4 times<\/td>\n<td>558 nm<\/td>\n<td>562 nm<\/td>\n<\/tr>\n<tr>\n<td>RDB14363<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14363\">pMALuci2Luc<\/a><\/td>\n<td>Malaysian Luciola sp. \u203b1<\/a><\/td>\n<td>2 times<\/td>\n<td>556 nm<\/td>\n<td>557 nm<\/td>\n<\/tr>\n<tr>\n<td>RDB14362<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14362\">pSflaRE1Luc<\/a><\/td>\n<td>Stenocladius flavipennis<\/a><\/td>\n<td>2 times<\/td>\n<td>608 nm<\/td>\n<td>609 nm<\/td>\n<\/tr>\n<tr>\n<td>RDB14361<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14361\">pPsagRE1Luc<\/a><\/td>\n<td>Pristolycus sagulatus<\/a><\/td>\n<td>2 times<\/td>\n<td>605 nm<\/td>\n<td>606 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>\u74b0\u5883\u8981\u56e0\u306b\u4f5c\u7528\u3055\u308c\u306b\u304f\u3044\u9ad8\u3044\u5b89\u5b9a\u6027\u304c\u3042\u308a\u307e\u3059<\/h3>\n<p>pH \u5909\u5316\u3084\u6e29\u5ea6\u5909\u5316\u306a\u3069\u306b\u8d77\u56e0\u3059\u308b\u767a\u5149\u8272\u3078\u306e\u5f71\u97ff\u304c\u5c11\u306a\u304f\u3001\u5b89\u5b9a\u3057\u305f\u89e3\u6790\u7d50\u679c\u3092\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td colspan=\"2\">\u5927\u8178\u83cc\u3067\u767a\u73fe\u3055\u305b\u7cbe\u88fd\u3057\u305f\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3068GTA Buffer\uff08\u6700\u7d42\u6fc3\u5ea6200mM\uff09\u3001ATP\uff08\u6700\u7d42\u6fc3\u5ea62mM\uff09\u3001MgSO4\uff08\u6700\u7d42\u6fc3\u5ea62mM\uff09\u3001D-Luciferin\uff08\u6700\u7d42\u6fc3\u5ea61mM\uff09\u3092\u542b\u3080\u53cd\u5fdc\u6db2\u3067\u767a\u5149\u3055\u305b\u305f\u3002\u5de6\u300125\u2103\u3002\u53f3\u300137\u2103\u3002\n<\/td>\n<\/tr>\n<tr>\n<td>PmatLuc1<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_003.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>DkumLuc1<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_004.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>MALuci2Luc<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_007.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>PsagRE1Luc<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_005.png\" width=\"500\"><\/td>\n<\/tr>\n<tr>\n<td>SflaRE1Luc<\/td>\n<td><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/inv0039_006.png\" width=\"500\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u203b1\u3000\u751f\u7269\u591a\u69d8\u6027\u6761\u7d04\u306b\u57fa\u3065\u304dPerak State Development Corporation, Nimura Genetic Solutions \u304a\u3088\u3073\u30aa\u30ea\u30f3\u30d1\u30b9\u682a\u5f0f\u4f1a\u793e\u306e\u5171\u540c\u958b\u767a\u3002<br \/>\n(\u6ce8) \u753b\u50cf\u3001\u306a\u3089\u3073\u306b\u30c7\u30fc\u30bf\u306f\u3001\u30aa\u30ea\u30f3\u30d1\u30b9\u682a\u5f0f\u4f1a\u793e\u304b\u3089\u63d0\u4f9b\u3092\u53d7\u3051\u3001\u7de8\u96c6\u3057\u3066\u63b2\u8f09\u3002<br \/>\n&nbsp;<\/p>\n<hr \/>\n<p><a name=\"kim\"><\/a><\/p>\n<h3>Artificial Luciferase Variants<\/h3>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19616<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19616\">ALuc23<\/a><\/td>\n<td>Expression vector of artificial luciferase variant ALuc23 derived from the copepod gene database for mammalian cell imaging.<\/td>\n<td>Kim, S.B. et al., 2013<\/td>\n<\/tr>\n<tr>\n<td>RDB19617<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19617\">ALuc49<\/a><\/td>\n<td>Expression vector of artificial luciferase variant ALuc49 derived from the copepod gene database for mammalian cell imaging.<\/td>\n<td>Kim, S.B. et al., 2017<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Reference<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Kim, S.B. et al. (2013) Bioconjug. Chem. 24 (12): 2067-2075. PMID 24237362<\/li>\n<li>Kim, S.B. et al. (2017) ACS Comb. Sci. 19 (9): 594-599. PMID 28742969<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<p><a name=\"JNC\"><\/a><\/p>\n<h3>JNC Corporation&#8217;s Resource<\/h3>\n<h4>Aequorea  victoria Aequorin<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19836<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19836\">pAQ-B<\/a><\/td>\n<td>Expression vector of A. victoria Aequorin, wild type.<\/td>\n<td>Inouye, S. et al., 1985<\/td>\n<\/tr>\n<tr>\n<td>RDB19830<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19830\">pJN-opAQ<\/a><\/td>\n<td>Expression vector of A. victoria Aequorin, codon-optimized for human (opAQ).<\/td>\n<td>Inouye, S. et al., 1985<\/td>\n<\/tr>\n<tr>\n<td>RDB19847<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19847\">pJNC-opAQ<\/a><\/td>\n<td>Mammalian expression vector of A. victoria Aequorin, codon-optimized (opAQ).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19848<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19848\">pJNC-COsp-opAQ<\/a><\/td>\n<td>Mammalian expression vector of human cytochrome C oxidase signal peptide sequence(COsp) and A. victoria Aequorin, codon-optimized (opAQ).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Clytia gregaria ClytinI and ClytinII<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19837<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19837\">pCL41<\/a><\/td>\n<td>Expression vector of C. gregaria ClytinI, wild type.<\/td>\n<td>Inouye, S. et al., 1993<\/td>\n<\/tr>\n<tr>\n<td>RDB19838<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19838\">pCL31<\/a><\/td>\n<td>Expression vector of C. gregaria ClytinII, wild type.<\/td>\n<td>Inouye, S., 2008<\/td>\n<\/tr>\n<tr>\n<td>RDB19831<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19831\">pJN-opCLII<\/a><\/td>\n<td>Expression vector of C. gregaria ClytinII, codon-optimized for human (opCLII).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19849<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19849\">pJNC-opCLII<\/a><\/td>\n<td>Mammalian expression vector of C. gregaria ClytinII, codon-optimized (opCLII).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19850<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19850\">pJNC-COsp-opCLII<\/a><\/td>\n<td>Mammalian expression vector of human cytochrome C oxidase signal peptide sequence(COsp) and C. gregaria ClytinII, codon-optimized (opCLII).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Renilla reniformis luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19853<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19853\">pJNC-opRL<\/a><\/td>\n<td>Mammalian expression vector of R. reniformis luciferase, codon-optimized (opRLuc). <\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<tr>\n<td>RDB19854<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19854\">pJNC-opRL547<\/a><\/td>\n<td>Mammalian expression vector of R. reniformis Red-shiffted luciferase, codon-optimized (opRLuc547), 547 nm peak.<\/td>\n<td>Unpublished bioresource<\/td>\n<\/tr>\n<tr>\n<td>RDB19832<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19832\">pJN-RLBP<\/a><\/td>\n<td>Expression vector of R. reniformis luciferin-binding protein (RLBP).<\/td>\n<td>Inouye, S. et al., 2007<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Luciola cruciata luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19833<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19833\">pJN-opLcLuc<\/a><\/td>\n<td>Expression vector of L. cruciata firefly luciferase, codon-optimized for human (opLcLuc).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4> Photinus pyralis luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19834<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19834\">pJN-opPyLuc<\/a><\/td>\n<td>Expression vector of P. pyralis firefly luciferase, codon-optimized for human (opPyLuc).<\/td>\n<td>Inouye, S. et al., 2015<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Oplophorus gracilorostris luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19840<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19840\">pKAZ-412<\/a><\/td>\n<td>Expression vector of O. gracilorostris 19 kDa Protein of Oplophorus Luciferase (KAZ), catalytic component.<\/td>\n<td>Inouye, S. et al., 2000<\/td>\n<\/tr>\n<tr>\n<td>RDB19841<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19841\">pJN-eKAZ<\/a><\/td>\n<td>Expression vector of O. gracilorostris enhanced 19 kDa Protein of Oplophorus Luciferase (eKAZ).<\/td>\n<td>Inouye, S. et al., 2014<\/td>\n<\/tr>\n<tr>\n<td>RDB19851<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19851\">pJNC-eKAZ<\/a><\/td>\n<td>Mammalian expression vector of O. gracilorostris enhanced 19 kDa Protein of Oplophorus Luciferase (eKAZ).<\/td>\n<td>Inouye, S. et al., 2014<\/td>\n<\/tr>\n<tr>\n<td>RDB19852<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19852\">pJNC-GLsp-eKAZ<\/a><\/td>\n<td>Mammalian expression vector of signal peptide sequence of G. princeps Gaussia luciferase (GLsp) and O. gracilorostris enhanced 19 kDa Protein of Oplophorus Luciferase (eKAZ).<\/td>\n<td>Inouye, S. et al., 2014<\/td>\n<\/tr>\n<tr>\n<td>RDB19842<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19842\">pOL23<\/a><\/td>\n<td>Expression vector of O. gracilorostris 35 kDa protein of Oplophorus luciferase, unknown function.<\/td>\n<td>Inouye, S. et al., 2000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Gaussia princeps luciferase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19845<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19845\">pJNC-hINS-GLuc<\/a><\/td>\n<td>Mammalian expression vector of human preproinsulin (hINS) and G. princeps Gaussia luciferase (GLuc).<\/td>\n<td>Suzuki, T. et al., 2011a<\/td>\n<\/tr>\n<tr>\n<td>RDB19846<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19846\">pJNC-hMMP2-GLuc<\/a><\/td>\n<td>Mammalian expression vector of human matrix metalloprotease-2 (hMMP2) and G. princeps Gaussia luciferase (GLuc).<\/td>\n<td>Suzuki, T. et al., 2011b<\/td>\n<\/tr>\n<tr>\n<td>RDB19855<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19855\">pJNC-pGLuc<\/a><\/td>\n<td>Mammalian expression vector of G. princeps Gaussia luciferase, codon-optimized (GLuc).<\/td>\n<td>Inouye, S. et al., 2016<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Vibrio fischeri NAD(P)H-flavin oxidoreductase<\/h4>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB19835<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19835\">pJN-FRase<\/a><\/td>\n<td>Expression vector of V. fischeri NAD(P)H-flavin oxidoreductase (FRase).<\/td>\n<td>Inouye, S., 1994<\/td>\n<\/tr>\n<\/tbody>\n<table>\n<h4>Reference<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Inouye, S. et al. (1985) Proc. Natl. Acad. Sci. U S A. 82 (10): 3154-3158. PMID 3858813<\/li>\n<li>Inouye, S. et al. (1993) FEBS Lett. 315 (3): 343-346. PMID 8422928<\/li>\n<li>Inouye, S. (1994) FEBS Lett. 347 (2-3): 163-168. PMID 8033996<\/li>\n<li>Inouye, S. et al. (2000) FEBS Lett. 481 (1): 19-25. PMID 10984608<\/li>\n<li>Inouye, S. et al. (2007) Protein Expr. Purif. 52: 66-73. PMID 16997571<\/li>\n<li>Inouye, S. J. Biochem. 143 (5):711-717, 2008. PMID 18296715<\/li>\n<li>Inouye, S. et al. (2014)Biochem. Biophys. Res. Commun. 445 (1): 157-162. PMID 24491536<\/li>\n<li>Inouye, S. et al. (2015) Protein Expr. Purif. 109: 47-54. PMID 25665506<\/li>\n<li>Inouye, S. et al. (2016) Protein Expr. Purif. 128: 93-100. PMID 27506135<\/li>\n<li>Suzuki, T. et al. (2011a) Anal. Bichem. 415 (2): 182-189. PMID 21477579<\/li>\n<li>Suzuki, T. et al. (2011b) Plos One. 6 (9): e25243. PMID 21969874<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"bacteria\"><\/a><\/p>\n<h2>Bacterial luciferase<\/h2>\n<h3>Vibrio harveyi luciferase<\/h3>\n<table>\n<tbody>\n<tr>\n<th Align=\"Left\">Catalog #<\/th>\n<th Align=\"Left\">Name of clone<\/th>\n<th Align=\"Left\">Running title<\/th>\n<th Align=\"Left\">Publication<\/th>\n<\/tr>\n<tr>\n<td>RDB18951<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18951\">pHLUX20<\/a><\/td>\n<td>Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain.<\/td>\n<td>Iizumi, T., 1998; Iizumi, T., 1997<\/td>\n<\/tr>\n<\/tbody>\n<table>\n<h4>Reference<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Iizumi, T. et al. (1998) Appl. Environ. Microbiol. 64 (10): 3656-3662. PMID 9758781<\/li>\n<li>Iizumi, T. and Nakamura, K. (1997) Appl. Environ. Microbiol. 63 (5): 1777-1784. PMID 9143112<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"control\"><\/a><\/p>\n<h3>Control vector<\/h3>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of resource<\/th>\n<th>Type<\/th>\n<\/tr>\n<tr>\n<td>RDB19350<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19350\">pGL4.14-E1bTATA<\/a><br \/>\nLuciferase reporter vector of the minimal adenovirus E1b promoter sequence.<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB03988<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03988\">pCMVluc+<\/a><br \/>\nExpression vector of firefly luciferase<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06411<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06411\">pGL3-RSV<\/a><br \/>\nExpression vector of firefly luciferase, RSV promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB03989<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03989\">pCAluc+<\/a><br \/>\nExpression vector of firefly luciferase<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02443<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02443\">pCAcc-Luc+<\/a><br \/>\nExpressing luciferase under the control of CAG promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02444<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02444\">pLRICALuc+<\/a><br \/>\nShuttle vector to generate recombinant adenovirus<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02968<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02968\">pTcf7wtluc<\/a><br \/>\nReporter plasmid carring TCF-binding consensus sequence<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02969<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02969\">pTcf7mtluc<\/a><br \/>\nReporter plasmid carring TCF-binding consensus sequence<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB07031<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07031\">ptk-luc<\/a><br \/>\nReporter construct with luciferase expression under tk promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB07032<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07032\">pFGF4tk-luc<\/a><br \/>\nReporter construct with luciferase expression under FGF4 and tk promoter<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB02453<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02453\">AxCA Luc+<\/a><br \/>\nRecombinant adenovirus expressing luciferase gene<\/td>\n<td><img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB04026<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB04026\">pKM2L<\/a><br \/>\nReporter construct with Renilla luciferase gene<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB05549<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB05549\">pKM2L-pvTK<\/a><br \/>\nHSV herpes simplex virus thymidine kinase (HSV-TK)<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB05550<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB05550\">pKM2L-pvSV40<\/a><br \/>\nSV40 simian virus 40 early enhancer\/promoter region (SV40)<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB05551<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB05551\">pKM2L-pvCMV<\/a><br \/>\nCMV cytomegalovirus immediate enhancer\/promoter (CMV)<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06408<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06408\">pRL-CMV mut1<\/a><br \/>\nExpression vector of Renilla luciferase, mutated CMV promoter<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06409<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06409\">pRL-CMV mut2<\/a><br \/>\nExpression vector of Renilla luciferase, mutated CMV promoter<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<tr>\n<td>RDB06410<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06410\">pRL-RSV<\/a><br \/>\nExpression vector of Renilla luciferase, RSV promoter<\/td>\n<td><img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<hr \/>\n<p style=\"text-align: left;\">2025.07.21 (T.M. GRP0025j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9ad8\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc Akaluc \u65b0\u898f\u958b\u767a\u306e\u8d64\u8272\u767a\u5149\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc \u30db\u30bf\u30eb\u306e\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u751f\u547d\u73fe\u8c61\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u767a\u5149\u57fa\u8cea\u306e\u7d44\u7e54\u900f\u904e\u6027\u304c\u4f4e\u3044\u3068\u3044\u3046\u6b20\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u7406\u7814\u8133\u795e\u7d4c\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u5bae\u8107 \u6566\u53f2\u5148\u751f\u3001\u5ca9\u91ce \u667a\u5148\u751f\u3001\u96fb\u6c17\u901a\u4fe1\u5927\u5b66\u5927\u5b66\u306e\u7267 \u660c\u6b21\u90ce\u5148\u751f\u3089\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u751f\u304d\u305f\u52d5\u7269\u500b\u4f53\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u4eba\u5de5\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0AkaBLI\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002 AkaBL [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":6265,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6275","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6275","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6275"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6275\/revisions"}],"predecessor-version":[{"id":6279,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6275\/revisions\/6279"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6265"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6275"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}