{"id":6260,"date":"2025-07-21T13:53:09","date_gmt":"2025-07-21T04:53:09","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6260"},"modified":"2025-12-22T08:16:13","modified_gmt":"2025-12-21T23:16:13","slug":"crisperja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/regurationja\/crisperja","title":{"rendered":"\u30b2\u30ce\u30e0\u7de8\u96c6 CRISPR\/Cas9"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>CRISPR\/Cas\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853<\/h2>\n<p>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001 \u6a19\u7684\u907a\u4f1d\u5b50\u3092\u5207\u65ad\u30fb\u6539\u5909\u3067\u304d\u308bCRISPR\/Cas\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u30c4\u30fc\u30eb\u3092\u53ce\u96c6\u30fb\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u5f53\u958b\u767a\u5ba4\u3067\u63d0\u4f9b\u3059\u308b\u7814\u7a76\u30c4\u30fc\u30eb\u306e\u591a\u304f\u306f\u3001\u56fd\u5185\u5916\u306e\u7814\u7a76\u8005\u306b\u3088\u3063\u3066\u4f5c\u88fd\u3055\u308c\u3001\u7814\u7a76\u6210\u679c\u304c\u8ad6\u6587\u3068\u3057\u3066\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u5b9f\u7e3e\u3042\u308b\u30ea\u30bd\u30fc\u30b9\u3067\u3059\u3002<br \/>\n\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u30c4\u30fc\u30eb\u306e\u63d0\u4f9b\u306e\u3054\u4f9d\u983c\u306e\u307f\u306a\u3089\u305a\u3001\u3054\u5bc4\u8a17\u3067\u306e\u3054\u5229\u7528\u3082\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"#\">CRISPR\/Cas3\u30b7\u30b9\u30c6\u30e0<\/a><\/li>\n<li><a href=\"#Cas9_plasmid\">Cas9\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9<\/a><\/li>\n<li><a href=\"#Cas9_adenovirus\">Cas9\u767a\u73fe\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9<\/a><\/li>\n<li><a href=\"#guide_RNA\">\u30ac\u30a4\u30c9RNA\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc<\/a><\/li>\n<li><a href=\"#donor\">\u907a\u4f1d\u5b50\u633f\u5165\u7528\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc<\/a><script>newImage(2024,11,23)<\/script><\/li>\n<li><a href=\"#CRISPR_evaluation\">\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u691c\u8a3c\u7528\u30d7\u30e9\u30b9\u30df\u30c9<\/a><\/li>\n<\/ul>\n<h3>CRISPR\/Cas3\u30b7\u30b9\u30c6\u30e0<\/h3>\n<p style=\"text-decoration: underline;\">CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.<\/p>\n<p>Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., Mashimo, T.<br \/>\nNat. Commun. 10 (1): 5302 (2019). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31811138\">PubMed PMID 31811138<\/a>.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB18212<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18212\">pCAG-All-in-one-hCascade<\/a><\/td>\n<td>Expression vector of CRISPR-associated complex for antiviral defense for genome editing tool.<\/td>\n<\/tr>\n<tr>\n<td>RDB18213<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18213\">pPB-CAG-hCas3<\/a><\/td>\n<td>Expression vector of Cas3 with bpNLS, CAG promoter for genome editing tool.<\/td>\n<\/tr>\n<tr>\n<td>RDB18214<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18214\">pBS-U6-crRNA-empty<\/a><\/td>\n<td>Expression vector of crRNA, U6 promoter for genome editing tool.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<a name=\"Cas9_plasmid\"><\/a><\/p>\n<h3>Cas9\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9<\/h3>\n<p style=\"text-decoration: underline;\">ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.<\/p>\n<p>Yoshimi, K., Kunihiro, Y., Kaneko, T., Nagahora, H., Voigt, B., Mashimo, T.<br \/>\nNat. Commun. 7: 10431 (2016). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26786405\">PubMed PMID 26786405<\/a>.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th style=\"text-align: center;\">Catalog number<\/th>\n<th style=\"text-align: center;\">Name of resource<\/th>\n<th style=\"text-align: center;\">Description<\/th>\n<\/tr>\n<tr>\n<td>RDB13130<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13130\">T7-NLS hCas9-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\nT7-NLS-hCAS9-pA \u306f\u3001poly A\u914d\u5217\u3092\u4ed8\u52a0\u3059\u308b\u3053\u3068\u3067Cas9\u304c\u5b89\u5b9a\u3057\u3066\u767a\u73fe\u3059\u308bin vitro\u8ee2\u5199\u7528\u306e\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002\u8ee2\u5199\u3057\u305fCas9 mRNA\u3068\u5171\u306b\u9577\u93961\u672c\u9396DNA\u3092\u30de\u30a6\u30b9\u306a\u3089\u3073\u306b\u30e9\u30c3\u30c8\u306e\u53d7\u7cbe\u5375\u306b\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3(Yoshimi, K. et al., 2016)\u3001\u3042\u308b\u3044\u306f\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3(Miyasaka, Y. et al., 2018)\u3059\u308b\u3053\u3068\u3067\u3001\u30b5\u30a4\u30ba\u306e\u5927\u304d\u306a\u5916\u6765\u907a\u4f1d\u5b50\u3092\u9ad8\u3044\u52b9\u7387\u3067\u30ce\u30c3\u30af\u30a4\u30f3\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"http:\/\/resou.osaka-u.ac.jp\/ja\/research\/2016\/20160120_1\">\u30de\u30a6\u30b9\u30fb\u30e9\u30c3\u30c8\u7b49\u306e\u907a\u4f1d\u5b50\u6539\u5909\u52b9\u7387\u3092\u5411\u4e0a\u3055\u305b\u308b\u65b0\u3057\u3044\u6280\u8853\u3092\u958b\u767a<\/a><\/li>\n<\/ul>\n<\/table>\n<hr width=\"50%\" \/>\nT7-NLS hCas9-pA (cat.# RDB13130)\u3092\u6539\u5909\u3057\u305fCas9-poly(A)\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\n<div align=\"center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB13130_01.png\" width=\"550\" \/>\n<\/div>\n<table>\n<tbody>\n<tr>\n<td><strong>Catalog no.<\/strong><\/td>\n<td><strong>Name of resource<\/strong><\/td>\n<td><strong>Description<\/strong><\/td>\n<\/tr>\n<tr>\n<td>RDB14419<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14419\">T7-NLS-hCAS9(D10A)-NLS-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<tr>\n<td>RDB14420<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14420\">T7-NLS-hCAS9(ver1.1)-NLS-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<tr>\n<td>RDB14421<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14421\">T7-NLS-hCAS9(D10A_ver1.1)-NLS-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<tr>\n<td>RDB14657<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14657\">T7-NLS-hCAS9-NLS-P2A-EGFP-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<tr>\n<td>RDB14658<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14658\">T7-NLS-hCAS9-NLS-P2A-mCherry-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<tr>\n<td>RDB14602<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14602\">T7-3xFLAG-NLS-hCAS9-NLS-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<tr>\n<td>RDB14604<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14604\">T7-3xFLAG-NLS-hCAS9-Cdt1-NLS-pA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-decoration: underline;\">Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes.<script>newImage(2021,5,13)<\/script>\n<p>Mizuno-Iijima, S., Ayabe, S., Kato, K., Matoba, S., Ikeda, Y., Dinh, T.T.H., Le, H.T., Suzuki, H., Nakashima, K., Hasegawa, Y., Hamada, Y., Tanimoto, Y., Daitoku, Y., Iki, N., Ishida, M., Ibrahim, E.A.E., Nakashiba, T., Hamada, M., Murata, K., Miwa, Y., Okada-Iwabu, M., Iwabu, M., Yagami, K.I., Ogura, A., Obata, Y., Takahashi, S., Mizuno, S., Yoshiki, A., Sugiyama, F.<br \/>\nMethods 191: 23-31 (2021). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32334080\">PubMed PMID 32334080<\/a>.<\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>Catalog no.<\/strong><\/td>\n<td><strong>Name of resource<\/strong><\/td>\n<td><strong>Description<\/strong><\/td>\n<\/tr>\n<tr>\n<td>RDB14406<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14406\">px330-mC<\/a><\/td>\n<td>Expression vector of sgRNA with hSpCas9-Cdt1(mouse) fusion protein.<\/td>\n<\/tr>\n<tr>\n<td>RDB14423<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14423\">pT7-hCas9-mC-polyA<\/a><\/td>\n<td>Cas9-poly(A) expressing improved plasmid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells.<\/p>\n<p>Sakakura, M., Ohta, S., Yagi, M., Tanaka, A., Norihide, J., Woltjen, K., Yamamoto, T., Yamada, Y.<br \/>\nBiochem. Biophys. Res. Commun. 519 (4): 705-713 (2019).<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31543342\">PubMed PMID 31543342<\/a>.<\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>Catalog no.<\/strong><\/td>\n<td><strong>Name of resource<\/strong><\/td>\n<td><strong>Description<\/strong><\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,1,31)<\/script>RDB18386<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18386\">px330-PPP<\/a><\/td>\n<td>Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">A genome editing vector that enables easy selection and identification of knockout cells.<\/p>\n<p>Nagasaki, A., Kato, Y., Meguro, K., Yamagishi, A., Nakamura, C., Uyeda, T.Q.P.<br \/>\nPlasmid 98: 37-44 (2018). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30196057\">PubMed PMID 30196057<\/a>.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th style=\"text-align: center;\">Catalog number<\/th>\n<th style=\"text-align: center;\">Name of resource<\/th>\n<th style=\"text-align: center;\">Description<\/th>\n<\/tr>\n<tr>\n<td>RDB16763<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16763\">pGedit<\/a><\/td>\n<td>Plasmid vector for high efficiency genome editing oligo DNA containing target sequence.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div align=\"center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB16763.png\" width=\"550\" \/>\n<\/div>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u7523\u696d\u6280\u8853\u7dcf\u5408\u7814\u7a76\u6240\u306e\u8fd1\u6c5f\u8c37\u514b\u88d5\u5148\u751f\u3001\u9577\u5d0e\u6643\u5148\u751f\u7b49\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u305f\u3001Cas9\u304a\u3088\u3073gRNA \u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002 <\/li>\n<li>\u30b2\u30ce\u30e0\u7de8\u96c6\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u304c\u81f4\u6b7b\u3001\u3042\u308b\u3044\u306f\u691c\u51fa\u304c\u5bb9\u6613\u306a\u8868\u73fe\u578b\u3092\u793a\u3055\u306a\u3044\u3088\u3046\u306a\u5834\u5408\u3001\u6a19\u7684\u5916\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3092\u6291\u3048\u308b\u3053\u3068\u3067\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3068\u81f4\u6b7b\u907a\u4f1d\u5b50\u540c\u5b9a\u306b\u5fc5\u8981\u306a\u52b4\u529b\u3092\u5c11\u3057\u3067\u3082\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30e9\u30f3\u30c0\u30e0\u306b\u30b2\u30ce\u30e0\u306b\u633f\u5165\u3055\u308c\u308b\u30d9\u30af\u30bf\u30fc\u6570\u304c\u591a\u3044\u3068\u30b2\u30ce\u30e0\u3078\u306e\u904e\u5270\u306aCas9\u9175\u7d20\u306e\u30a2\u30bf\u30c3\u30af\u304c\u8d77\u304d\u3001Off-target\u306e\u539f\u56e0\u3068\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u9577\u5d0e\u5148\u751f\u3089\u304c\u958b\u767a\u3057\u305fpGedit (cat# RDB16763)\u306fblasticidin S \u8010\u6027\u907a\u4f1d\u5b50 (Bsr) \u3092\u6301\u3064Cas9\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3067\u3059\u3002\u3088\u308a\u77ed\u6642\u9593\u3067\u7d30\u80de\u3092\u9078\u629e\u3067\u304d\u308bblasticidin S \u3092\u4f7f\u3044\u3001blasticidin S\u306e\u57f9\u5730\u6dfb\u52a0\u309224\uff5e48\u6642\u9593\u306e\u307f\u306b\u9650\u5b9a\u3059\u308b\u3053\u3068\u3067\u30d9\u30af\u30bf\u30fc\u306e\u30b2\u30ce\u30e0\u3078\u306e\u30a4\u30f3\u30c6\u30b0\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u6291\u3048\u3001\u3055\u3089\u306bEGFP\u3092Bsr\u3068\u878d\u5408\u3057\u3066\u767a\u73fe\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30d9\u30af\u30bf\u30fc\u306e\u6b8b\u5b58\u3092\u30e2\u30cb\u30bf\u30fc\u3067\u304d\u308b\u3088\u3046\u306a\u5de5\u592b\u3092\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001sgRNA\u30b9\u30ab\u30d5\u30a9\u30fc\u30eb\u30c9\u5185\u306b\u3042\u308bRNA polymerase III terminal signal \uff08TTTT\uff09\u3092\u53d6\u308a\u9664\u304f\u3053\u3068\u3067sgRNA\u91cf\u3092\u5897\u52a0\u3055\u305b\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306e\u5897\u52a0\u3092\u671f\u5f85\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u3053\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u6709\u7528\u6027\u3092\u307f\u308b\u305f\u3081\u3001\u30d2\u30c8\u9aa8\u8089\u816b\u4e0a\u76ae\u7d30\u80deU2OS\u3092\u7528\u3044\u30662\u7a2e\u985e\u306e\u30d2\u30c8\u7d30\u80de\u8cea\u30a2\u30af\u30c1\u30f3\u907a\u4f1d\u5b50\u3001ACTB\u3068ACTG1\u306b\u5bfe\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u3092pGedit\u306b\u633f\u5165\u3057\u3001\u3053\u306e\uff12\u3064\u306e\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7d30\u80de\u682a\u3092\u53d6\u5f97\u3059\u308b\u5b9f\u9a13\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u305d\u308c\u305d\u308c\u306e\u907a\u4f1d\u5b50\u306b\u3064\u3044\u3066\u7d0420\u306e\u5019\u88dc\u7d30\u80de\u304b\u3089\u5c11\u306a\u304f\u3068\u30825\u500b\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7d30\u80de\u682a\u3092\u5f97\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors.<\/p>\n<p>Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.<br \/>\nCell Rep. 15 (1): 210-218 (2016). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27052166\">PubMed PMID 27052166<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<td class=\"t02\"><strong>Catalog no.<\/strong><\/td>\n<td class=\"t02\"><strong>Name of resource<\/strong><\/td>\n<td class=\"t02\"><strong>Description<\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\">RDB13917<\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13917\">AAVS1 T2 CRIPR in pX330<\/a><\/td>\n<td class=\"t02\">A CRISPR\/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Please visit <a href=\"https:\/\/brc.riken.jp\/dna\/GRP0016e\">the Auxin Inducible Degron (AID) System<\/a>.<\/p>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">CRISPR\/Cas9-mediated gene knockout in the ascidian Ciona intestinalis.<\/p>\n<p>Sasaki, H., Yoshida, K., Hozumi, A., Sasakura, Y.<br \/>\nDev. Growth Differ.56 (7): 499-510 (2014). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25212715\">PubMed PMID 25212715<\/a>.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th style=\"text-align: center;\">Catalog number<\/th>\n<th style=\"text-align: center;\">Name of resource<\/th>\n<th style=\"text-align: center;\">Description<\/th>\n<\/tr>\n<tr>\n<td>RDB12994<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12994\">pHTB Cas9<\/a><\/td>\n<td>In vitro production of Cas9 mRNA.<\/td>\n<\/tr>\n<tr>\n<td>RDB12999<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12999\">pSPCiEF1>Cas9<\/a><\/td>\n<td>Expression plasmid of Cas9.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u7b51\u6ce2\u5927\u5b66\u306e\u7b39\u5009\u9756\u5fb3\u5148\u751f\u7b49\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u305f\u3001\u30ab\u30bf\u30e6\u30a6\u30ec\u30a4\u30dc\u30e4\u5b9f\u9a13\u7528Cas9-poly(A)\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002 <\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<a name=\"Cas9_adenovirus\"><\/a><\/p>\n<h3>Cas9\u767a\u73fe\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9<\/h3>\n<p style=\"text-decoration: underline;\">\nConstruction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR\/Cas9 and in vivo genome editing.<\/p>\n<p>Nakanishi, T., Maekawa, A., Suzuki, M., Tabata, H., Sato, K., Mori, M., Saito, I.<br \/>\nSci. Rep. 11 (1): 3961 (2021).  <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33597562\">PubMed PMID 33597562<\/a>.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB19290<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19290\">AxCBCas9<\/a><script>newImage(2022,01,20)<\/script><\/td>\n<td>Recombinant adenovirus expressing spCas9 under the control of CB promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB19291<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19291\">AxCBNC9<\/a><script>newImage(2022,01,20)<\/script><\/td>\n<td>Recombinant adenovirus expressing spCas9 nickase under the control of CB promoter.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n\u7406\u7814BRC\u306f\u3001<a href=\"https:\/\/web.brc.riken.jp\/ja\/about\/link\/broad_institute\">\u7c73\u56fdBroad Institute of MIT and Harvard\u304b\u3089\u3001CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u30c4\u30fc\u30eb\u306e\u5bc4\u8a17\u306e\u53d7\u3051\u5165\u308c\u3001\u306a\u3089\u3073\u306b\u63d0\u4f9b\u306e\u8a31\u8afe<\/a>\u3092\u53d7\u3051\u3066\u304a\u308a\u307e\u3059\u3002\u3053\u306e\u8a31\u8afe\u306b\u3088\u308a\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u30ea\u30bd\u30fc\u30b9\u306e\u5bc4\u8a17\u3092\u53d7\u5165\u308c\u3001\u7dad\u6301\u30fb\u7ba1\u7406\u30fb\u4fdd\u5b58\u3057\u3001Broad Institute\u306b\u3088\u308a\u6307\u5b9a\u3055\u308c\u305f\u55b6\u5229\u76ee\u7684\u5229\u7528\u3078\u306e\u63d0\u4f9b\u7981\u6b62\u306e\u6761\u9805\u3092\u4ed8\u8a18\u3057\u305f\u5c02\u7528\u306e\u751f\u7269\u907a\u4f1d\u8cc7\u6e90\u63d0\u4f9b\u540c\u610f\u66f8\u3092\u7de0\u7d50\u3057\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u3067\u3001\u975e\u55b6\u5229\u6a5f\u95a2\u306e\u5b66\u8853\u7814\u7a76\u3078\u63d0\u4f9b\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n<hr \/>\n<p>&nbsp;<a name=\"guide_RNA\"><\/a><\/p>\n<h3>\u30ac\u30a4\u30c9RNA\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc<\/h3>\n<p style=\"text-decoration: underline;\">\nCRISPR screening in human trophoblast stem cells reveals both shared and distinct aspects of human and mouse placental development.<\/p>\n<p>Shimizu, T., Oike, A., Kobayashi, E.H., Sekiya, A., Kobayashi, N., Shibata, S., Hamada, H., Saito, M., Yaegashi, N., Suyama, M., Arima, T., Okae, H.<br \/>\nProc. Natl. Acad. Sci. U.S.A. 120 (51): e2311372120 (2023). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/38085778\">PubMed PMID  38085778<\/a>.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB21099<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB21099\">pCS-hU6-Neo(V2)<\/a><script>newImage(2025,12,21)<\/script><\/td>\n<td>Guide RNA expression vector under the control of human U6 (hU6) promoter.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p>&nbsp;<a name=\"donor\"><\/a><\/p>\n<h3>\u907a\u4f1d\u5b50\u633f\u5165\u7528\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc<\/h3>\n<p style=\"text-decoration: underline;\">Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system.<script>newImage(2024,11,23)<\/script>\n<p>Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y.<br \/>\nCell Rep. Methods 3 (12):100662 (2023). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/38086384\">PubMed PMID 38086384<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB19575<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19575\">pUlrTK<\/a><\/td>\n<td>Genome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, &#038; 5UAS<\/td>\n<\/tr>\n<tr>\n<td>RDB19574<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19574\">pUC wLG3-T-EZ(ERV)<\/a><\/td>\n<td>Genome editing tool to tag tdTomato at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR.<\/td>\n<\/tr>\n<tr>\n<td>RDB19573<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19573\">pUC wLG3-G-EZ(ERV)<\/a><\/td>\n<td>Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR.<\/td>\n<\/tr>\n<tr>\n<td>RDB19569<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19569\">pUCtk2x2<\/a><\/td>\n<td>Universal donor vector for double-tk CRISPR\/Cas9 knock-in system. Kpn I\/Asp718I site is available for insertion of homology arm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a.<\/p>\n<p>Ishibashi, R., Maki, R., Kitano, S., Miyachi, H., Toyoshima, F.<br \/>\nSci. Rep. 12 (1): 17775 (2022). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/36272994\">PubMed PMID 36272994<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB20013<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB20013\">pCriMGET_9-12a<\/a><\/td>\n<td>Plasmid vector to construct a donor cassette for knock-in of long external DNA cleaved by both CRISPR-Cas9 and CRISPR-Cas12a.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p style=\"text-decoration: underline;\">Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence.<\/p>\n<p>Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F.<br \/>\nSci. Rep. 10 (1): 14120 (2020). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32839482\">PubMed PMID 32839482<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB19318<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19318\">pCriMGET_v1<\/a><\/td>\n<td>Plasmid vector to clone a targeting cassette. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo.<\/td>\n<\/tr>\n<tr>\n<td>RDB19319<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19319\">pX330-syn-crRNA-TS-sgRNA<\/a><\/td>\n<td>Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318).<\/td>\n<\/tr>\n<tr>\n<td>RDB19320<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19320\">pCriMGET_v1-AAVS1-SA-Neo-pA<\/a><\/td>\n<td>Targeting construct to insert Splicing Acceptor (SA)-neomycin resistance gene (neo)-bGHpA into the human AAVS1 locus by the homologous recombination.<\/td>\n<\/tr>\n<tr>\n<td>RDB19321<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19321\">pCriMGET_v1-Tbx3-3xFlag-2A-EGFP<\/a><\/td>\n<td>Targeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene.<\/td>\n<\/tr>\n<tr>\n<td>RDB19322<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19322\">pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP<\/a><\/td>\n<td>Targeting construct ot insert IRES-hDTR-P2A-EGFP into 3&#8217;UTR region of mouse Clec4f gene.<\/td>\n<\/tr>\n<tr>\n<td>RDB19323<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19323\">pX330-AAVS1-sgRNA<\/a><\/td>\n<td>Expression vector for hCas9 and guideRNA of human AAVS1 locus.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing.<\/p>\n<p>Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y.<br \/>\nStem Cell Res. 53: 102363 (2021). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/34087992\">PubMed PMID 34087992<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB18672<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18672\">pUC-TEZ<\/a><\/td>\n<td>tdTomato Reporter\/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.<\/td>\n<\/tr>\n<tr>\n<td>RDB18670<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18670\">pCRtk2x2NN<\/a><\/td>\n<td>Backbone vector for constructing donor vector with double HSV-thymidine kinase.<\/td>\n<\/tr>\n<tr>\n<td>RDB17468<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17468\">pUC tk2<\/a><\/td>\n<td>Backbone vector for constructing donor vector with single HSV-thymidine kinase.<\/td>\n<\/tr>\n<tr>\n<td>RDB17462<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17462\">pUC GEB<\/a><\/td>\n<td>EGFP Reporter\/Blasticidin resistance selection marker cassette for constructing knock-in donor vector.<\/td>\n<\/tr>\n<tr>\n<td>RDB17461<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17461\">pUC TEBl-EcoRV<\/a><\/td>\n<td>tdTomato Reporter\/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.<\/td>\n<\/tr>\n<tr>\n<td>RDB17460<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17460\">pUC GEB-EcoRV<\/a><\/td>\n<td>EGFP Reporter\/Blasticidin resistance selection marker cassette for constructing knock-in donor vector.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes).<\/p>\n<p>Murakami, Y., Ansai, S., Yonemura, A., Kinoshita, M.<br \/>\nZoological Lett. 3: 10 (2017). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28694996\">PubMed PMID 28694996<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15409<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15409\">pBaitD-gap43-linker-EGFP<\/a><\/td>\n<td>Donor plasmid for targeted insertion into the gap43 locus of Japanese medaka by the BaitD system.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Precise in-frame integration of exogenous DNA mediated by CRISPR\/Cas9 system in zebrafish.<\/p>\n<p>Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A.<br \/>\nSci. Rep. 5: 8841 (2015). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25740433\">PubMed PMID 25740433<\/a>.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB13480<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13480\">pCS2P-tyr-mCherry-donor<\/a><\/td>\n<td>Donor vector for integrating mCherry into zebrafish tyrosinase locus.<\/td>\n<\/tr>\n<tr>\n<td>RDB13481<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13481\">pCS2P-krtt1c19e-linker-eGFP-mut-donor<\/a><\/td>\n<td>Donor vector for integrating eGFP mutant into zebrafish krtt1c19e locus<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u7406\u5316\u5b66\u7814\u7a76\u6240\u751f\u547d\u30b7\u30b9\u30c6\u30e0\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08QBiC\uff09\u306e\u4e45\u91ce\u60a0\u5148\u751f\u7b49\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u5bc4\u8a17\u3055\u308c\u305f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u5fdc\u7528\u3057\u3066\u5916\u6765\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u30de\u30fc\u30ab\u30fc\u3067\u3059 (Hisano, Y. et al., 2015)\u3002 RDB13481\u306f\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u306e\u8868\u76ae\u7d30\u80de\u306b\u767a\u73fe\u3059\u308b\u30b1\u30e9\u30c1\u30f3\u3092GFP\u306b\u3088\u3063\u3066\u53ef\u8996\u5316\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nHisano, Y. et al., Precise in-frame integration of exogenous DNA mediated by CRISPR\/Cas9 system in Zebrafish. Sci Rep. 5: 8841 (2015). [PMID: 25740433]<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.<\/p>\n<p>Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.<br \/>\nCell Rep. 15 (1): 210-218 (2016). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27052166\">PubMed PMID 27052166<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<td class=\"t02\" rowspan=\"2\"><strong>Selection marker<\/strong><\/td>\n<td class=\"t02\"colspan=\"3\"><strong>Attached tag at the N-terminus<\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>mClover<\/td>\n<td class=\"t02\"><strong>mCherry2<\/td>\n<td class=\"t02\"><strong>S tag-3FLAG<\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>Hygro<\/strong><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16814\">pMK376<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16815\">pMK377<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16813\">pMK375<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<td class=\"t02\" rowspan=\"2\"><strong>Selection marker<\/strong><\/td>\n<td class=\"t02\"colspan=\"3\"><strong>Attached tag at the C-terminus<\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>mClover<\/strong><\/td>\n<td class=\"t02\"><strong>mCherry2<\/strong><\/td>\n<td class=\"t02\"><strong>S tag-3FLAG<\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>Neo<\/strong><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13920\">pMK277<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13923\">pMK280<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13926\">pMK283<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>Hygro<\/strong><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13921\">pMK278<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13924\">pMK281<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13927\">pMK284<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>BSD<\/strong><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16816\">pMK388<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16817\">pMK389<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16818\">pMK390<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\"><strong>Bsr<\/strong><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13922\">pMK279<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13925\">pMK282<\/a><\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13928\">pMK285<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\u306e\u9418\u5dfb\u5c06\u4eba\u5148\u751f\u7b49\u306b\u3088\u308a\u958b\u767a\u3055\u305f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u5fdc\u7528\u3057\u3066\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u907a\u4f1d\u5b50\u3084\u85ac\u5264\u8010\u6027\u907a\u4f1d\u5b50\u3092\u30b2\u30ce\u30e0\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u30de\u30fc\u30ab\u30fc\u3067\u3059\u3002<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"CRISPR_evaluation\"><\/a><\/p>\n<h3>\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u691c\u8a3c\u7528\u30d7\u30e9\u30b9\u30df\u30c9<\/h3>\n<p style=\"text-decoration: underline;\">Generation of CRISPR\/Cas9-mediated bicistronic knock-in ins1-cre driver mice.<\/p>\n<p>Hasegawa, Y., Hoshino, Y., Ibrahim, A.E., Kato, K., Daitoku, Y., Tanimoto, Y., Ikeda, Y., Oishi, H., Takahashi, S., Yoshiki, A., Yagami, K., Iseki, H., Mizuno, S., Sugiyama, F.<br \/>\nExp. Anim. 65 (3): 319-327 (2016). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27053096\">PubMed PMID 27053096<\/a>.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\">\n<tbody>\n<tr>\n<th style=\"text-align: center;\">Catalog number<\/th>\n<th style=\"text-align: center;\">Name of resource<\/th>\n<th style=\"text-align: center;\">Description<\/th>\n<\/tr>\n<tr>\n<td>RDB13948<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13948\">p2color<\/a><\/td>\n<td>Genome editing tool for confirming the activity of CRISPR\/Cas9 system.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.<\/p>\n<p>Mashiko, D., Fujihara, Y., Satouh, Y., Miyata, H., Isotani, A., Ikawa, M.<br \/>\nSci. Rep. 3: 3355 (2013). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24284873\">PubMed PMID 24284873<\/a>.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th style=\"text-align: center;\">Catalog number<\/th>\n<th style=\"text-align: center;\">Name of resource<\/th>\n<th style=\"text-align: center;\">Description<\/th>\n<\/tr>\n<tr>\n<td>RDB13532<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13532\">pCAG-EGxxFP<\/a><\/td>\n<td>Plasmid clone for validation of sgRNA on double strand breaks by observation of green fluorescence.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div align=\"center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB13532_01.png\" width=\"550\" \/>\n<\/div>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u5927\u962a\u5927\u5b66\u306e\u4f0a\u5ddd\u6b63\u4eba\u5148\u751f\u7b49\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u5bc4\u8a17\u3055\u308c\u305f\u3001\u86cd\u5149\u5f37\u5ea6\u3092\u5229\u7528\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u3092\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u30d9\u30af\u30bf\u30fc\u3067\u3059 (Mashiko, D. et al., 2013)\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u5c0e\u5165\u3057\u305f\u3044\u6a19\u7684\u914d\u5217\u3092PCR\u306b\u3088\u308a\u5897\u5e45\u3057\u305f\u5f8c\u3001EGFP\u914d\u5217\u4e2d\u306b\u7d44\u8fbc\u307f\u307e\u3059\u3002\u3053\u306e\u30af\u30ed\u30fc\u30f3\u3068Cas9\u767a\u73fe\u30af\u30ed\u30fc\u30f3\u3001\u306a\u3089\u3073\u306b\u30ac\u30a4\u30c9RNA\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u540c\u6642\u306b\u52d5\u7269\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3068\u3001\u6a19\u7684\u914d\u5217\u306e\u4e8c\u672c\u9396DNA\u306e\u5207\u65ad\u306b\u4f34\u3044\u3001EGFP\u914d\u5217\u304c\u4fee\u5fa9\u3055\u308c\u3066\u7dd1\u8272\u86cd\u5149\u3092\u751f\u3058\u307e\u3059\u3002<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">pCAG-NexxoR, the plasmid to enhance the productivity of aimed gene KO cell by CRISPR-Cas9 genome editing.<\/p>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\">\n<tbody>\n<tr>\n<td class=\"t02\"><strong>Catalog no.<\/strong><\/td>\n<td class=\"t02\"><strong>Name of resource<\/strong><\/td>\n<td class=\"t02\"><strong>Description<\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"t02\">RDB16803<\/td>\n<td class=\"t02\"><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16803\">pCAG-NexxoR<\/a><\/td>\n<td class=\"t02\">Selection plasmid for the positive clone of CRISPR\/Cas9 system in the culture cells.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u56fd\u7acb\u7cbe\u795e\u30fb\u795e\u7d4c\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc \u7167\u5149\u5b9f\u52a0\u5148\u751f\u3001\u6a4b\u6238\u548c\u592b\u5148\u751f\u3089\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u305f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8a2d\u8a08\u901a\u308a\u306b\u8d77\u304d\u305f\u7d30\u80de\u3092\u85ac\u5264\u8010\u6027\u306b\u3088\u308a\u52b9\u7387\u7684\u306b\u6fc3\u7e2e\u3067\u304d\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002pCAG-EGxxFP (RDB13532)\u306eEGFP\u3092\u30cd\u30aa\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u306b\u7f6e\u304d\u63db\u3048\u6539\u5909\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002<\/li>\n<li>\u307e\u305a\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u3092\u542b\u3080\u30b2\u30ce\u30e0\u65ad\u7247\u3092\u633f\u5165\u3057\u305fpCAG-NexxoR \u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u3068\u3057\u3066\u4f5c\u88fd\u3057\u307e\u3059\u3002\u6b21\u306b\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u5fc5\u8981\u306a\u9175\u7d20\u304a\u3088\u3073\u30ac\u30a4\u30c9RNA\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u3068\u5171\u306b\u7d30\u80de\u306b\u5c0e\u5165\u3057\u307e\u3059\u3002\u305d\u306e\u5f8cG418\u3092\u52a0\u3048\u305f\u57f9\u5730\u3067\u8010\u6027\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u4e0a\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8d77\u304d\u305f\u7d30\u80de\u306e\u307f\u304c\u751f\u80b2\u3057\u9ad8\u52b9\u7387\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8d77\u304d\u305f\u7d30\u80de\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n\u7167\u5149\u5148\u751f\u3089\u306b\u3088\u308bC2C12\u57f9\u990a\u7d30\u80de\u3092\u7528\u3044\u305f\u30d1\u30a4\u30ed\u30c3\u30c8\u5b9f\u9a13\u3067\u306f\u3001\u7d0498%\u306e\u52b9\u7387\u3067\u5909\u7570\u4f53\u304c\u5f97\u3089\u308c\u3001\u5909\u7570\u4f53\u4e2d\u306e\u7d04\uff12\uff05\u304c\u30db\u30e2\u5909\u7570\u4f53\u3067\u3057\u305f\u3002\uff08\u56f3\uff11\uff09<\/li>\n<li>\u5b9f\u9a13\u7d50\u679c\u306e\u4e00\u4f8b<br \/>\n\u30b2\u30ce\u30e0\u7de8\u96c6\u7528\u30d7\u30e9\u30b9\u30df\u30c9pX330-\u30ac\u30a4\u30c9RNA\uff08pX330\u306b\u30ac\u30a4\u30c9RNA\u914d\u5217\u3092\u633f\u5165)\u3001pCAG-EGxxFP-target (pCAG-EGxxFP\u306btarget\u914d\u5217\u3092\u633f\u5165)\u3092C2C12\u7d30\u80de\u306bCotransfection\u3057\u300124\u6642\u9593\u5f8c\u304b\u3089G418\u6dfb\u52a0\u57f9\u5730\u3067\u57f9\u990a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3068\u304d\u3001pCAG-NexxoR-target\uff08pCAG-NexxoR\u306btarget\u914d\u5217\u3092\u633f\u5165\uff09\u3001\u3042\u308b\u3044\u306fpCAG-NeoR (G418\u8010\u6027\u907a\u4f1d\u5b50\u767a\u73fe\u30d9\u30af\u30bf\u30fc) \u3082Transfection\u3057\u307e\u3057\u305f\u3002<br \/>\n\u3053\u306e\u7d50\u679c\u304b\u3089\u3001\u85ac\u5264\u8010\u6027\u907a\u4f1d\u5b50\u306e\u5171\u767a\u73fe\u3060\u3051\u3067\u306f\u9078\u5225\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u793a\u3055\u308cNexxoR\u306e\u6709\u7528\u6027\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\uff08\u5b9f\u9a13\u7d50\u679c\u306f\u3001\u56fd\u7acb\u7cbe\u795e\u30fb\u795e\u7d4c\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u7167\u5149\u5148\u751f\u306b\u63d0\u4f9b\u3057\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\uff09<br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB16803_1en.png\" width=\"550\" \/><br \/>\n\u56f3\uff11\uff0e\u5de6\uff1a G418\u6dfb\u52a048\u6642\u9593\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u751f\u3058\u305f\u7d30\u80de\uff08GFP\u967d\u6027\uff09\u304c\u9078\u5225\u3055\u308c\u3001GFP\u9670\u6027\u7d30\u80de\u306f\u307b\u307c\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<br \/>\n\u4e2d\uff1a G418\u6dfb\u52a0\u7121\u3067\u306fGFP\u9670\u6027\u7d30\u80de\u304c\u591a\u304f\u898b\u3089\u308c\u307e\u3057\u305f\u3002<br \/>\n\u53f3\uff1a pCAG-NeoR\u306eCotransfection\u3067\u306f\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u751f\u3058\u305f\u7d30\u80de\uff08GFP\u967d\u6027\uff09\u304c\u9078\u5225\u3055\u308c\u305aGFP\u9670\u6027\u7d30\u80de\u304c\u591a\u304f\u898b\u3089\u308c\u307e\u3057\u305f\u3002\n<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<p style=\"text-decoration: underline;\">Quantitative assay for TALEN activity at endogenous genomic loci.<\/p>\n<p>Hisano, Y., Ota, S., Arakawa, K., Muraki, M., Kono, N., Oshita, K., Sakuma, T., Tomita, M., Yamamoto, T., Okada, Y., Kawahara, A.<br \/>\nBiol. Open. 2 (4): 363-367 (2013). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23616919\">PubMed PMID 23616919<\/a>.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog number<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB12261<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12261\">pBR-lacZ alpha<\/a><\/td>\n<td>Plasmid clone for quantitative assay for genome editing at endogenous genomic loci.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div align=\"center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB12261_01.png\" width=\"550\" \/>\n<\/div>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6982\u8981<br \/>\n\u7406\u5316\u5b66\u7814\u7a76\u6240\u751f\u547d\u30b7\u30b9\u30c6\u30e0\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08QBiC\uff09\u306e\u4e45\u91ce\u60a0\u5148\u751f\u7b49\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u5bc4\u8a17\u3055\u308c\u305f\u3001\u9752\u8272\u30fb\u767d\u8272\u30b3\u30ed\u30cb\u30fc\u306e\u51fa\u73fe\u6bd4\u7387\u306b\u3088\u3063\u3066\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u3092\u8a55\u4fa1\u3067\u304d\u308b\u30d9\u30af\u30bf\u30fc\u3067\u3059 (Hisano, Y. et al., 2013)\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u8a66\u884c\u5f8c\u3001\u6a19\u7684\u914d\u5217\u3092PCR\u306b\u3088\u308a\u5897\u5e45\u3057\u3001\u672c\u30af\u30ed\u30fc\u30f3\u306elacZ\u3092\u30b3\u30fc\u30c9\u3059\u308b\u5869\u57fa\u914d\u5217\u4e2d\u306b\u633f\u5165\u3057\u307e\u3059\u3002\u3053\u306e\u969b\u3001\u5897\u5e45\u3057\u305f\u914d\u5217\u304c\u30a4\u30f3\u30d5\u30ec\u30fc\u30e0\u306b\u306a\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3057\u3066\u4f5c\u88fd\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u7528\u3044\u3066\u5927\u8178\u83cc\u3092\u5f62\u8cea\u8ee2\u63db\u3057\u3001X-gal\u3001IPTG\u3001\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3092\u6dfb\u52a0\u3057\u305f\u5bd2\u5929\u57f9\u5730\u306b\u64ad\u7a2e\u3059\u308b\u3068\u3001\u5897\u5e45\u3057\u305f\u6a19\u7684\u914d\u5217\u304c\u91ce\u751f\u578b\u30a2\u30ec\u30eb\u3067\u3042\u308c\u3070\u9752\u8272\u30b3\u30ed\u30cb\u30fc\u304c\u3001\u7de8\u96c6\u304c\u8d77\u3053\u308a\u8aad\u307f\u67a0\u306b\u30ba\u30ec\u304c\u751f\u3058\u305f\u5834\u5408\u306f\u3001\u767d\u8272\u30b3\u30ed\u30cb\u30fc\u304c\u51fa\u73fe\u3057\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nHisano,Y. et al., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open, 2, 363-367 (2013). [PMID: 23616919]<\/li>\n<\/ul>\n<hr \/>\n<h3><span style=\"text-decoration: underline;\">\u30fb\u30b2\u30ce\u30e0\u7de8\u96c6\u30ea\u30bd\u30fc\u30b9\u306e\u3054\u5bc4\u8a17\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059<\/span><\/h3>\n<p>\u3053\u3053\u6570\u5e74\u3067\u5287\u7684\u306a\u9032\u6b69\u3092\u9042\u3052\u3066\u3044\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u306f\u3001\u57fa\u790e\u7814\u7a76\u3001\u5fdc\u7528\u7814\u7a76\u554f\u308f\u305a\u3001\u751f\u547d\u79d1\u5b66\u3092\u652f\u3048\u308b\u6838\u3068\u306a\u308b\u6280\u8853\u306e\u4e00\u3064\u306b\u307e\u3067\u767a\u5c55\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u305d\u308c\u306b\u4f34\u3063\u3066\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u30ea\u30bd\u30fc\u30b9\u958b\u767a\u8005\u3078\u306e\u5206\u4e0e\u4f9d\u983c\u304c\u5897\u52a0\u3059\u308b\u3053\u3068\u304c\u4e88\u60f3\u3055\u308c\u307e\u3059\u3002\u30ea\u30bd\u30fc\u30b9\u3092\u7814\u7a76\u8005\u9593\u3067\u5171\u6709\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u5b9f\u9a13\u306e\u518d\u73fe\u6027\u304c\u691c\u8a3c\u3055\u308c\u3001\u65b0\u3057\u3044\u7814\u7a76\u6210\u679c\u3078\u3068\u7e4b\u304c\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u5206\u4e0e\u306b\u304b\u304b\u308b\u4f5c\u696d\u306f\u500b\u3005\u306e\u7814\u7a76\u8005\u306e\u5927\u304d\u306a\u8ca0\u62c5\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u3002\u5f53\u958b\u767a\u5ba4\u306b\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u3067\u3001\u5206\u4e0e\u306b\u304b\u304b\u308b\u8ca0\u62c5\u3092\u8efd\u6e1b\u3057\u3001\u7814\u7a76\u6d3b\u52d5\u306b\u5c02\u5fc3\u3057\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u662f\u975e\u3068\u3082\u30ea\u30bd\u30fc\u30b9\u306e\u5bc4\u8a17\u3092\u3054\u691c\u8a0e\u3044\u305f\u3060\u304d\u307e\u3059\u3088\u3046\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<\/p>\n<p><a href=\"\/ja\/kitaku\">\u300c\u5bc4\u8a17\u30fb\u8b72\u6e21\u306e\u7533\u8fbc\u307f\u300d<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p style=\"text-align: left;\">2025.12.21 (N.N. GRP0024j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR\/Cas\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853 \u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001 \u6a19\u7684\u907a\u4f1d\u5b50\u3092\u5207\u65ad\u30fb\u6539\u5909\u3067\u304d\u308bCRISPR\/Cas\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u30c4\u30fc\u30eb\u3092\u53ce\u96c6\u30fb\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u5f53\u958b\u767a\u5ba4\u3067\u63d0\u4f9b\u3059\u308b\u7814\u7a76\u30c4\u30fc\u30eb\u306e\u591a\u304f\u306f\u3001\u56fd\u5185\u5916\u306e\u7814\u7a76\u8005\u306b\u3088\u3063\u3066\u4f5c\u88fd\u3055\u308c\u3001\u7814\u7a76\u6210\u679c\u304c\u8ad6\u6587\u3068\u3057\u3066\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u5b9f\u7e3e\u3042\u308b\u30ea\u30bd\u30fc\u30b9\u3067\u3059\u3002 \u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u30c4\u30fc\u30eb\u306e\u63d0\u4f9b\u306e\u3054\u4f9d\u983c\u306e\u307f\u306a\u3089\u305a\u3001\u3054\u5bc4\u8a17\u3067\u306e\u3054\u5229\u7528\u3082\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002 CRISPR\/Cas3\u30b7\u30b9\u30c6\u30e0 Cas9\u767a [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":6248,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6260","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6260"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6260\/revisions"}],"predecessor-version":[{"id":6578,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6260\/revisions\/6578"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6248"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}