{"id":6253,"date":"2025-07-21T13:45:21","date_gmt":"2025-07-21T04:45:21","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6253"},"modified":"2025-07-21T13:47:03","modified_gmt":"2025-07-21T04:47:03","slug":"auxindegronja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/regurationja\/auxindegronja","title":{"rendered":"\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3\u6cd5 (AID system) \u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u5236\u5fa1"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<ul>\n<li>\u30aa\u30fc\u30ad\u30b7\u30f3\u4f9d\u5b58\u7684\u306b\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\u3067\u3001\u767a\u73fe\u5236\u5fa1\u3057\u307e\u3059\u3002<\/li>\n<li>\u57f9\u990a\u7d30\u80de\u306b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u7531\u6765aid\u30bf\u30b0\u3092\u4ed8\u52a0\u3057\u305f\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30a4\u30cdF-box\u30bf\u30f3\u30d1\u30af\u8ceaTIR1\u3092\u3042\u3089\u304b\u3058\u3081\u540c\u6642\u306b\u767a\u73fe\u3055\u305b\u3066\u304a\u304d\u3001\u57f9\u5730\u306b\u30aa\u30fc\u30ad\u30b7\u30f3\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067\u767a\u73fe\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n<h2>\u30ea\u30bd\u30fc\u30b9\u4e00\u89a7<\/h2>\n<p><span style=\"text-decoration: underline;\">Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system.<\/span><br \/>\nHatoyama, Y., Homma, Y., Hiragi, S., Fukuda, M.<br \/>\nJ. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB19431<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19431\">pMRX-bsr-FLAG-OsTIR1<\/a><script>newImage(2022,03,25)<\/script><\/td>\n<td>Retroviral expression vector of oryza sativa TIR-L1 tagged with FLAG.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p><span style=\"text-decoration: underline;\">A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.<\/span><br \/>\nNishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U,. Fukagawa, T.<br \/>\nNucleic Acids Res. 48 (18): e108, 2020. PMID: 32941625.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB19226<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19226\">pAID1.2-CMV-NmScarlet-mAID<\/a><script>newImage(2022,01,12)<\/script><\/td>\n<td>Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by CMV promoter for the super-sensitive AID system.<\/td>\n<\/tr>\n<tr>\n<td>RDB19227<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19227\">pAID1.2-EF1a-NmScarlet-mAID<\/a><script>newImage(2022,01,12)<\/script><\/td>\n<td>Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by EF1 alpha promoter for the super-sensitive AID system.<\/td>\n<\/tr>\n<tr>\n<td>RDB19228<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19228\">pAIDFA-CMV-NmScarlet-mAID<\/a><script>newImage(2022,01,12)<\/script><\/td>\n<td>Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by CMV promoter for the super-sensitive AID system.<\/td>\n<\/tr>\n<tr>\n<td>RDB19229<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19229\">pAIDFA-EF1a-NmScarlet-mAID<\/a><script>newImage(2022,01,12)<\/script><\/td>\n<td>Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by EF1 alpha promoter for the super-sensitive AID system.<\/td>\n<\/tr>\n<tr>\n<td>RDB19230<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19230\">pAID1.2-CMV-linearizing in pX330<\/a><script>newImage(2022,01,12)<\/script><\/td>\n<td>Expression vector of Cas9 protein and sgRNA to linearize pAID-CMV plasmids in vivo.<\/td>\n<\/tr>\n<tr>\n<td>RDB19231<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19231\">pAID-EF1a- linearizing in pX330<\/a><script>newImage(2022,01,12)<\/script><\/td>\n<td>Expression vector of Cas9 protein and sgRNA to linearize to pAID-EF1 alpha plasmids in vivo.<\/td>\n<\/tr>\n<table>\n<tbody>\n<hr \/>\n<p><span style=\"text-decoration: underline;\">Application of auxin-inducible degron technology to mouse oocyte activation with PLC\u03b6.<\/span><br \/>\nMiura, K., Matoba, S., Ogonuki, N., Namiki, T., Ito, J., Kashiwazaki, N., Ogura, A.<br \/>\nJ. Reprod. Dev. 64 (4): 319-326 (2018). PMID 29731504.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB16762<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16762\">pcDNA-Ostir1-pA<\/a><\/td>\n<td>Expression vector of transport inhibitor response 1 derived from Oryza sativa (OsTIR).<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p style=\"text-decoration: underline;\"><script>newImage(2020,12,22)<\/script>The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.<\/p>\n<p>Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T.<br \/>\nNat. Commun. 11: 5701, 2020.<\/p>\n<p style=\"text-decoration: underline;\">Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole.<\/p>\n<p>Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T.<br \/>\nMethods pii: S1046-2023 (18) 30331-1, 2019. PMID: 31026591.<\/p>\n<p style=\"text-decoration: underline;\">Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.<\/p>\n<p>Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.<br \/>\nCell Reports 15: 210-218, 2016. PMID: 27052166.<\/p>\n<h3>Table 1. CsTIR1 and AtAFB2 expression vectors and helper plasmid<\/h3>\n<h4>a. CsTIR1 and AtAFB2 expression vectors<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB13918<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13918\">pMK232<\/a><\/td>\n<td>OsTIR1 expression vector, PURO<\/td>\n<\/tr>\n<tr>\n<td>RDB16811<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16811\">pMK364<\/a><\/td>\n<td>OsTIR1 expression vector, loxP-PURO-loxP<\/td>\n<\/tr>\n<tr>\n<td>RDB18337<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18337\">pMK381<\/a><script>newImage(2020,12,22)<\/script><\/td>\n<td>OsTIR1 F74G expression vector, loxP-PURO-loxP<\/td>\n<\/tr>\n<tr>\n<td>RDB18347<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18347\">pMK233<\/a><script>newImage(2020,12,22)<\/script><\/td>\n<td>AtAFB2 expression vector, loxP-PURO-loxP<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>b. Helper plasmid for knock-in a expression vector to the human AAVS1 site<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB13917<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13917\">AAVS1 T2 CRIPR in pX330<\/a><\/td>\n<td>A CRISPR\/Cas plasmid for making DNA dounle-strand break at the human AAVS1 locus.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>c. CsTIR1 expression vector of yeast<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB18341<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18341\">pMK198<\/a><script>newImage(2020,12,22)<\/script><\/td>\n<td>GAL-OsTIR1 expression vector of yeast<\/td>\n<\/tr>\n<tr>\n<td>RDB18342<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18342\">pMK419<\/a><script>newImage(2020,12,22)<\/script><\/td>\n<td>GAL-OsTIR1(F74G) expression vector of yeast<\/td>\n<\/tr>\n<tr>\n<td>RDB18343<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18343\">pMK425<\/a><script>newImage(2020,12,22)<\/script><\/td>\n<td>GAL-OsTIR1(F74A) expression vector of yeast<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h3>Table 2. Tags<\/h3>\n<h4>a. mAID tags, N-terminal tagging<\/h4>\n<table>\n<tbody>\n<tr>\n<th rowspan=\"2\">Selection<br \/>\nmarker<\/th>\n<th colspan=\"4\">Attached tag at the N-terminus<\/th>\n<\/tr>\n<tr>\n<th>mAID<\/th>\n<th>mAID-mClover<\/th>\n<th>mAID-mCherry2<\/th>\n<th>mAID-Nluc<\/th>\n<\/tr>\n<tr>\n<tr>\n<th>Hygro<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16805\">pMK344<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16806\">pMK345<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16807\">pMK346<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16823\">pMK401<\/a><\/td>\n<\/tr>\n<tr>\n<th>BSD<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16808\">pMK347<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16809\">pMK348<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16810\">pMK349<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16824\">pMK407<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>b. mAID tags, C-terminal tagging<\/h4>\n<table>\n<tbody>\n<tr>\n<th rowspan=\"2\">Selection<br \/>\nmarker<\/th>\n<th colspan=\"4\">Attached tag at the C-terminus<\/th>\n<\/tr>\n<tr>\n<th>mAID<\/th>\n<th>mAID-mClover<\/th>\n<th>mAID-mCherry2<\/th>\n<th>mAID-Nluc<\/th>\n<\/tr>\n<tr>\n<th>Neo<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13929\">pMK286<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13932\">pMK289<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13935\">pMK292<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16822\">pMK394<\/a><\/td>\n<\/tr>\n<tr>\n<th>Hygro<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13930\">pMK287<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13933\">pMK290<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13936\">pMK293<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16825\">pMK408<\/a><\/td>\n<\/tr>\n<tr>\n<th>BSD<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16819\">pMK391<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16820\">pMK392<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16821\">pMK393<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16826\">pMK409<\/a><\/td>\n<\/tr>\n<tr>\n<th>Bsr<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13931\">pMK288<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13934\">pMK291<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13937\">pMK294<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h3>Table 3. Tags<\/h3>\n<h4>a. Tags without mAID, N-terminal tagging<\/h4>\n<table>\n<tbody>\n<tr>\n<th rowspan=\"2\">Selection<br \/>\nmarker<\/th>\n<th colspan=\"3\">Attached tag at the N-terminus<\/th>\n<\/tr>\n<tr>\n<th>mClover<\/th>\n<th>mCherry2<\/th>\n<th>S tag-3FLAG<\/th>\n<\/tr>\n<tr>\n<th>Hygro<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16814\">pMK376<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16815\">pMK377<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16813\">pMK375<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>b. Tags without mAID, C-terminal tagging<\/h4>\n<table>\n<tbody>\n<tr>\n<th rowspan=\"2\">Selection<br \/>\nmarker<\/th>\n<th colspan=\"3\">Attached tag at the C-terminus<\/th>\n<\/tr>\n<tr>\n<th>mClover<\/th>\n<th>mCherry2<\/th>\n<th>S tag-3FLAG<\/th>\n<\/tr>\n<tr>\n<th>Neo<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13920\">pMK277<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13923\">pMK280<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13926\">pMK283<\/a><\/td>\n<\/tr>\n<tr>\n<th>Hygro<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13921\">pMK278<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13924\">pMK281<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13927\">pMK284<\/a><\/td>\n<\/tr>\n<tr>\n<th>BSD<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16816\">pMK388<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16817\">pMK389<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16818\">pMK390<\/a><\/td>\n<\/tr>\n<tr>\n<th>Bsr<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13922\">pMK279<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13925\">pMK282<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13928\">pMK285<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h3>Table 4. <script>newImage(2020,12,22)<\/script>mAID and mIAA7 tagging specific gene<\/h3>\n<table>\n<tbody>\n<tr>\n<th>Gene<\/th>\n<th>Helper<\/th>\n<th>Tagging with Hygro marker<\/th>\n<th>Tagging with Neo marker<\/th>\n<\/tr>\n<tr>\n<td>BRD4<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18359\">BRD4-N CRISPR<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18358\">mAID-BRD4 donor<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>CTCF<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18355\">CTCF-C CRISPR<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18354\">CTCF-mAC donor (Hygro)<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18353\">CTCF-mAC donor (Neo)<\/a><\/td>\n<\/tr>\n<tr>\n<td>DHC1<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18352\">DHC1 CRISPR<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18349\">pMK297<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18348\">pMK296<\/a><\/td>\n<\/tr>\n<tr>\n<td>DHC1<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18352\">DHC1 CRISPR<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18351\">DHC1-mIAA7 Hygro<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18350\">DHC1-mIAA7 Neo<\/a><\/td>\n<\/tr>\n<tr>\n<td>RAD21<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18346\">RAD21 C-tag CIRPSR<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18345\">pMK265<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18344\">pMK262<\/a><\/td>\n<\/tr>\n<tr>\n<td>SMC2<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18357\">SMC2 CRISPR<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18356\">SMC2-mAC donor (Hygro)<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>TOP2A<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18362\">pMK312<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18361\">pMK322<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18360\">pMK321<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h3>Table 5. <script>newImage(2020,12,22)<\/script>Simultaneous expression of OsTIR1 and mAID-EGFP<\/h3>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<\/tr>\n<tr>\n<td>RDB18338<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18338\">pAAV-hSyn-OsTIR1(WT)<\/a><\/td>\n<td>Adeno-associated virus (AAV) expression vector of OsTIR1(wild type) and mAID-EGFP-NES marker with hSyn promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB18339<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18339\">pAAV-hSyn-OsTIR1(F74G)<\/a><\/td>\n<td>Adeno-associated virus (AAV) expression vector of OsTIR1(F74G) and mAID-EGFP-NES marker with hSyn promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB18340<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18340\">pAAV-hSyn-OsTIR1(F74A)<\/a><\/td>\n<td>Adeno-associated virus (AAV) expression vector of OsTIR1(F74A) and mAID-EGFP-NES marker with hSyn promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB18333<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18333\">pAY7<\/a><\/td>\n<td>TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-NLS marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB18334<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18334\">pAY15<\/a><\/td>\n<td>TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-NLS marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB18335<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18335\">pAY19<\/a><\/td>\n<td>TOL2 expression vector of OsTIR1(F74A) and mAID-EGFP-NLS marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB18363<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18363\">pMK427<\/a><\/td>\n<td>TOL2 expression vector of OsTIR1(WT) and mAID-EGFP-Nluc marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB18364<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18364\">pMK411<\/a><\/td>\n<td>TOL2 expression vector of OsTIR1(F74G) and mAID-EGFP-Nluc marker.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<hr \/>\n<p style=\"text-decoration: underline;\">An auxin-based degron system for the rapid depletion of proteins in nonplant cells.<\/p>\n<p>Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., Kanemaki, M.<br \/>\nNat. Methods., 6, 917-922, 2009. PMID: 19915560.<\/p>\n<table width=\"100%\" border=\"1\" cellspacing=\"0\" cellpadding=\"5\" bgcolor=\"#FFFFFF\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name of clone<\/th>\n<th>Short description<\/th>\n<th>Type<\/th>\n<\/tr>\n<tr>\n<td>RDB08468<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08468\">pNHK60<\/a><\/td>\n<td>Expression clone for an auxin-based degron (AID) system<\/td>\n<td>Plasmid<\/td>\n<\/tr>\n<tr>\n<td>RDB08469<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08469\">pMK106<\/a><\/td>\n<td>Expression clone for an auxin-based degron (AID) system<\/td>\n<td>Plasmid<\/td>\n<\/tr>\n<tr>\n<td>RDB08470<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08470\">pMK107<\/a><\/td>\n<td>Expression clone for an auxin-based degron (AID) system<\/td>\n<td>Plasmid<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul>\n<li>Supplementary Figure 11 for pMK106 (aid-CENPH)<\/li>\n<li>Supplementary Figure 11 for pMK107 (CENPH-aid)<\/li>\n<li>Supplementary figure 10 for pNHK60 (pIRES OsTIR1-EGFP-IAA17)<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" alt=\"construction images\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB08468a.png\" width=\"500\" height=\"326\" \/><\/p>\n<h4>\u53c2\u8003\u6587\u732e\u30fb\u95a2\u9023\u6587\u732e<\/h4>\n<ol>\n<li>Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U,. Fukagawa, T. A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108, 2020. PMID: 32941625.<\/li>\n<li>Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T. Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1, 2019. PMID: 31026591.<\/li>\n<li>Research highlights<br \/>\n<a href=\"https:\/\/www.nig.ac.jp\/nig\/ja\/2019\/05\/research-highlights_ja\/rh20190510.html\">\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3(AID)\u6cd5\u306e\u305f\u3081\u306eCRISPR\/Cas9\u3092\u5229\u7528\u3057\u305f\u65b0\u898f\u30bf\u30b0\u5c0e\u5165\u3068\u5206\u89e3\u963b\u5bb3\u5264\u306e\u958b\u767a<\/a><\/li>\n<li>Natsume, T., Nishimura, K., Minocherhomji, S., Bhowmick, R., Hickson, I.D., Kanemaki, M.T. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 31 (8): 816-829, 2017.. PMID: 28487407.<\/li>\n<li>Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Reports 15: 210-218, 2016. PMID: 27052166.<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"https:\/\/www.nig.ac.jp\/nig\/ja\/2017\/05\/research-highlights_ja\/20170510.html\">\u58ca\u308c\u305f\u300cDNA\u306e\u30d5\u30a1\u30b9\u30ca\u30fc\u300d\u3092\u4fee\u7406\u3059\u308b\u306b\u306f\uff1f\uff5e\u7d30\u80de\u304cDNA\u3092\u30b3\u30d4\u30fc\u3059\u308b\u969b\u306e\u30d0\u30c3\u30af\u30a2\u30c3\u30d7\u30b7\u30b9\u30c6\u30e0\u3092\u767a\u898b\uff5e<\/a><\/li>\n<li>Takagi, M., Natsume, T., Kanemaki, M.T., Imamoto, N. Perichromosomal protein Ki67 supports mitotic chromosome architecture. Genes Cells 21 (10): 1113-1124, 2016. PMID: 27610954.<\/li>\n<li>Samejima, K., Ogawa, H., Ageichik, A.V., Peterson, K.L., Kaufmann, S.H., Kanemaki, M.T., Earnshaw, W.C. Auxin-induced Rapid Degradation of Inhibitor of Caspase-activated DNase (ICAD) Induces Apoptotic DNA Fragmentation, Caspase Activation, and Cell Death: A CELL SUICIDE MODULE. J. Biol. Chem. 289 (45): 31617-31623, 2014. PMID: 25248749<\/li>\n<li>Rodriguez-Bravo, V., Maciejowski, J., Corona, J., Buch, H.K., Collin, P., Kanemaki, M.T., Shah, J.V., Jallepalli, P.V. Nuclear pores protect genome integrity by assembling a premitotic and Mad1-dependent anaphase inhibitor. Cell 156 (5): 1017-1031, 2014. PMID: 24581499<\/li>\n<li>Kanemaki, M.T. Frontiers of protein expression control with conditional degrons. Eur. J. Physiol., 465, 419-425, 2013. PMID: 23271452.<\/li>\n<li>Holland, A.J., Fachinetti, D., Han, J.S., Cleveland, D.W. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 109 (49): E3350-E3357, 2012. PMID: 23150568<\/li>\n<li>Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., Kanemaki, M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat. Methods., 6, 917-922, 2009. PMID: 19915560.<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/DEP007157\">Masato Kanemaki Lab Resources<\/a><\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"https:\/\/www.nig.ac.jp\/nig\/ja\/2016\/03\/research-highlights_ja\/20160325.html\">\u53d6\u308a\u9664\u3051\u3070\u50cd\u304d\u304c\u308f\u304b\u308b\uff01\uff5e\u7279\u5b9a\u306e\u30d2\u30c8\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d20\u65e9\u304f\u53d6\u308a\u9664\u3044\u3066\u6a5f\u80fd\u3092\u63a2\u308b\u65b9\u6cd5\u3092\u958b\u767a\uff5e <\/a><br \/>\n<a href=\"https:\/\/www.nig.ac.jp\/nig\/images\/research_highlights\/PR20160325.pdf\">\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\u8cc7\u6599 [PDF]<\/a><\/li>\n<li><a href=\"http:\/\/www.shigen.nig.ac.jp\/shigen\/news\/n_letter\/2011\/nl201107.pdf\">BioResource Now! Issue 7, July 2011<\/a> (PDF, in Japanese)<\/li>\n<li><a href=\"http:\/\/www.shigen.nig.ac.jp\/shigen\/news\/n_letter\/2011\/nl201107En.pdf\">BioResource Now! Issue 7, July 2011<\/a> (PDF, in English)<\/li>\n<\/ol>\n<p style=\"text-align: left;\">2025.07.21 (T.M. GRP0016j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30aa\u30fc\u30ad\u30b7\u30f3\u4f9d\u5b58\u7684\u306b\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\u3067\u3001\u767a\u73fe\u5236\u5fa1\u3057\u307e\u3059\u3002 \u57f9\u990a\u7d30\u80de\u306b\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u7531\u6765aid\u30bf\u30b0\u3092\u4ed8\u52a0\u3057\u305f\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30a4\u30cdF-box\u30bf\u30f3\u30d1\u30af\u8ceaTIR1\u3092\u3042\u3089\u304b\u3058\u3081\u540c\u6642\u306b\u767a\u73fe\u3055\u305b\u3066\u304a\u304d\u3001\u57f9\u5730\u306b\u30aa\u30fc\u30ad\u30b7\u30f3\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067\u767a\u73fe\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u3092\u8a98\u5c0e\u3057\u307e\u3059\u3002 \u30ea\u30bd\u30fc\u30b9\u4e00\u89a7 Establishment and analysis of conditional Rab1- and Rab5-kno [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":6248,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6253","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6253","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6253"}],"version-history":[{"count":4,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6253\/revisions"}],"predecessor-version":[{"id":6259,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6253\/revisions\/6259"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6248"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6253"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}