{"id":6215,"date":"2025-06-23T08:24:17","date_gmt":"2025-06-22T23:24:17","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6215"},"modified":"2025-06-23T08:24:37","modified_gmt":"2025-06-22T23:24:37","slug":"adenoja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/viralvecja\/adenoja","title":{"rendered":"\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<ul>\n<li><a href=\"#about_adeno\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066<\/a><\/li>\n<li><a href=\"#vector_adeno\">\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf<\/a><\/li>\n<li><a href=\"#reference_adeno\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599<\/a><\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"about_adeno\"><\/a><\/p>\n<p style=\"text-align: right;\"><img loading=\"lazy\" decoding=\"async\" alt=\"aden01\" src=\"https:\/\/dnaconda.riken.jp\/images\/aden01.png\" width=\"73\" height=\"66\" \/><\/p>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066<\/h3>\n<p>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001\u56fd\u5185\u306a\u3089\u3073\u306b\u6d77\u5916\u306b\u304a\u3044\u3066\u7523\u51fa\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u306e\u5bc4\u8a17\u3092\u3046\u3051\u3001\u691c\u5b9a\u5f8c\u4fdd\u5b58\u3057\u54c1\u8cea\u7ba1\u7406\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u7814\u7a76\u3067\u5b9f\u969b\u306b\u4f7f\u7528\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306a\u3089\u3073\u306b\u5f53\u958b\u767a\u5ba4\u3067\u72ec\u81ea\u306b\u958b\u767a\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u3001\u4ed6\u306b\u306f\u306a\u3044\u30e6\u30cb\u30fc\u30af\u306a\u3082\u306e\u304c\u591a\u6570\u3042\u308a\u307e\u3059\u3002<br \/>\n\u7406\u7814BRC\u30fb\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u304a\u3088\u3073\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30d9\u30af\u30bf\u30fc\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u307e\u3059\u3002<br \/>\n\u65b0\u305f\u306b\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u307e\u305f\u306f\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30d9\u30af\u30bf\u30fc\u7b49\u306e\u7814\u7a76\u6750\u6599\u3092\u958b\u767a\u3055\u308c\u305f\u65b9\u306f\u3001\u305c\u3072\u3001\u5f53\u65b9\u306b\u5fa1\u5bc4\u8a17\u4e0b\u3055\u3044\u307e\u3059\u3088\u3046\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<br \/>\n\u305f\u3060\u3057\u3001\u5f53\u30d0\u30f3\u30af\u306f\u3001\u75c5\u539f\u6027\u30a6\u30a4\u30eb\u30b9\u306f\u6271\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\u3042\u3089\u304b\u3058\u3081\u3054\u4e86\u627f\u9858\u3044\u307e\u3059\u3002<\/p>\n<hr \/>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u53d7\u53d6\u3063\u305f\u3089<\/h3>\n<p>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u53d7\u53d6\u3063\u3066\u304b\u3089\u7cbe\u88fd\u3059\u308b\u307e\u3067\u306e\u624b\u9806\u306e\u6982\u8981\u3067\u3059\u3002\u64cd\u4f5c\u624b\u9806\u306f<a href=\"\/ja\/wp-content\/uploads\/manual\/adenovirus3.pdf\" target=\"_blank\">\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300d<\/a>\u306b\u8a18\u8f09\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u5e45\n<ul>\n<li>\u7406\u7814DNA Bank\u304c\u63d0\u4f9b\u3059\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u3068\u3057\u3066\u304a\u9001\u308a\u81f4\u3057\u307e\u3059\u3002\u529b\u4fa1\u306f\u6e2c\u5b9a\u3057\u3066\u3044\u307e\u305b\u3093\u304c\uff0c\u304a\u304a\u3088\u305d10<sup>6-8<\/sup>pfu\/ml\u306e\u6db2\u3092100 \u03bcl \u304a\u9001\u308a\u3044\u305f\u3057\u307e\u3059\u3002\u304a\u53d7\u3051\u53d6\u308a\u5f8c\u306f\u76f4\u3061\u306b\u57f9\u990a\u3092\u958b\u59cb\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u76f4\u3061\u306b\u57f9\u990a\u304c\u3067\u304d\u306a\u3044\u5834\u5408\u306f-80\u2103\u3067\u4fdd\u7ba1\u3057\u3001\u306a\u308b\u3079\u304f\u65e9\u304f\u57f9\u990a\u3057\u3066\u4e0b\u3055\u3044\u3002<\/li>\n<li>\u64cd\u4f5c\u65b9\u6cd5\n<ul>\n<li>10 cm\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u30c7\u30a3\u30c3\u30b7\u30e5\u306b\u57f9\u990a\u3057\u305f293 \u7d30\u80de\uff08\u57f9\u990a\u6db210ml, \u7d049&#215;10<span style=\"vertical-align: super;\">6<\/span>cells\uff09\u3092\u7528\u610f\u3059\u308b\u3002<\/li>\n<li>\u30a6\u30a4\u30eb\u30b9\u6db2\u3092100 \u03bcl\u611f\u67d3\u3055\u305b\u308b\uff08\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300dProtocol C-1 ,3, 4 \u6b21\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u53c2\u7167\uff09<\/li>\n<li>\u7d043\uff5e4 \u65e5\u5f8c\u306b\u7d30\u80de\u304c\u6b7b\u6ec5\u3057\u305f\u3089\u3001\u7d0410ml\u306e\u57f9\u990a\u6db2\u3092\u7d30\u80de\u3054\u3068\uff08\u6b7b\u6ec5\u7d30\u80de\u3054\u3068\uff0950 mL\u30c1\u30e5\u30fc\u30d6\u306b\u56de\u53ce\u3059\u308b\u3002\u3059\u3050\u306b\u4f7f\u308f\u306a\u3044\u5834\u5408\u306f\u7c97\u30a6\u30a4\u30eb\u30b9\u6db2\u3068\u3057\u3066\u305d\u306e\u307e\u307e\uff0d80\u2103\u306b\u4fdd\u5b58\u3059\u308b\u3002\u3059\u3050\u306b\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u5bc6\u9589\u8d85\u97f3\u6ce2\u7834\u7815\u307e\u305f\u306f\u51cd\u7d50\u878d\u89e3\u3057\u3001\u30a6\u30a4\u30eb\u30b9\u3092\u904a\u96e2\u3055\u305b,9,400 g\u300110 \u5206\u30014\u2103\u3067\u9060\u5fc3\u3057\u3066\u4e0a\u6e05\u3092\u56de\u53ce\u3057\u30a6\u30a4\u30eb\u30b9\u6db2\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\uff08\u30a6\u30a4\u30eb\u30b9\u6db2\u7d0410ml\u304c\u5f97\u3089\u308c\u308b\uff09\u3002\u6b8b\u308a\u306f\u3001\u84cb\u304c\u30cd\u30b8\u5f0f\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u5206\u6ce8\u3057\u3066-80\u2103\u306b\u4fdd\u5b58\u3059\u308b\u3002 \u30fb\u3053\u306e\u5f8c\u3001\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300d\u306e\u300c\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u306e\u8d85\u9060\u5fc3\u306b\u3088\u308b\u5927\u91cf\u8abf\u88fd\u300d\u306b\u5f93\u3063\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u7cbe\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8f38\u9001\u306a\u3089\u3073\u306b\u4fdd\u5b58\u6642\u306e\u5b89\u5b9a\u6027\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/12036457\/\">Ugai, H. et al., Jpn. J. Cancer Res., 93, 598-603 (2002).<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u5897\u5e45\u306b\u306fHEK293\u7d30\u80de (RCB1637)\u3092\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/li>\n<li>\u914d\u5217\u3068\u69cb\u9020\u306e\u691c\u67fb\n<ul>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u9650\u3089\u305a\u3001\u7d44\u63db\u3048DNA\u30af\u30ed\u30fc\u30f3\u306f\u7a81\u7136\u5909\u7570\u3092\u8d77\u3053\u3059\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u7d44\u8fbc\u307f\u907a\u4f1d\u5b50\uff08\u30d5\u30a1\u30a4\u30d0\u30fc\u5909\u7570\u4f53\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u30d5\u30a1\u30a4\u30d0\u30fc\u9818\u57df\u3082\uff09\u306e\u6027\u72b6\u3092\u5b9f\u9a13\u306e\u524d\u306b\u3054\u81ea\u8eab\u3067\u8abf\u3079\u3066\u4e0b\u3055\u3044\u3002\u305d\u306e\u65b9\u6cd5\u3068\u3057\u3066\u3001PCR\u3001DNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3042\u308b\u3044\u306f\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u81ea\u5f8b\u5897\u6b96\u30a6\u30a4\u30eb\u30b9\u6df7\u5165\u306e\u691c\u67fb\n<ul>\n<li>\u7406\u7814DNA Bank\u306e\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u305d\u306e\u307b\u3068\u3093\u3069\u304cCOS-TPC\u6cd5\u306b\u3088\u308a\u4f5c\u88fd\u3055\u308c\u3066\u304a\u308a(Miyake S. et al., 1996)\u3001\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9(Replication Competent Adenovirus, RCA)\u304c\u6df7\u5165\u3059\u308b\u6050\u308c\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u5897\u6b96\u904e\u7a0b\u3067\u3082\u5bbf\u4e3b\u3067\u3042\u308bHEK293\u7d30\u80de\u306e\u6301\u3064E1\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u63db\u3048\u306b\u3088\u308a\u7372\u5f97\u3059\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u3057\u3088\u3046\u3059\u308b\u524d\u306b\u306f\u3001RCA\u304c\u5b58\u5728\u3057\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002Suzuki E et al. (2004)\u306b\u305d\u306e\u65b9\u6cd5\u3092\u63b2\u8f09\u3057\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/li>\n<li>\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u691c\u51fa\u306b\u306fHeLa\u7d30\u80de (RCB0007)\u3092\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u7cbe\u88fd\n<ul>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u7c21\u4fbf\u306a\u7cbe\u88fd\u6cd5\u306f\u3001<a href=\"\/ja\/wp-content\/uploads\/manual\/adenovirus3.pdf\" target=\"_blank\">\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300d<\/a>\u306e\u307b\u304b\u3001<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/15882984\/\">Ugai et al., Biochem. Biophys. Res. Commun. 331: 1053-1060 (2005)<\/a>\u3001 \u9d5c\u98fc\u3089\u3001\u5b9f\u9a13\u533b\u5b66\u3001\u7f8a\u571f\u793e\u300124, 981-985 (2006)\u306b\u8a18\u8f09\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<hr \/>\n<p><a name=\"vector_adeno\"><\/a><\/p>\n<h3>\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc<\/h3>\n<h4>\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u4f7f\u7528\u6642\u306e\u6ce8\u610f<\/h4>\n<ul>\n<li>\u7b2c\u4e8c\u7a2e\u4f7f\u7528\u7b49\u3092\u76ee\u7684\u3068\u3057\u305f\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u3067\u3059\u3002<\/li>\n<li>\u5bbf\u4e3b\u306e\u540d\u79f0\u306a\u3089\u3073\u306b\u7d44\u63db\u3048\u6838\u9178\u306e\u540d\u79f0\u306f\u3001\u5404\u30af\u30ed\u30fc\u30f3\u306e\u8a73\u7d30\u60c5\u5831\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\u3002<\/li>\n<li>\u672c\u30d9\u30af\u30bf\u30fc\u306f\u5b9a\u3081\u3089\u308c\u305f\u5b9f\u9a13\u6761\u4ef6\u4e0b\u3067\u5b9a\u3081\u3089\u308c\u305f\u5bbf\u4e3b\u7d30\u80de\u306b\u5c0e\u5165\u3057\u305f\u6642\u306b\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u7523\u751f\u3059\u308b\u69d8\u306b\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u308c\u4ee5\u5916\u306e\u6761\u4ef6\u4e0b\u3067\u30a6\u30a4\u30eb\u30b9\u304c\u7523\u751f\u3055\u308c\u308b\u53ef\u80fd\u6027\u306f\u5426\u5b9a\u3067\u304d\u307e\u305b\u3093\u3002\u5f93\u3044\u307e\u3057\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u4ee5\u5916\u306e\u76ee\u7684\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u4f7f\u7528\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u5341\u5206\u306b\u6ce8\u610f\u3057\u3066\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<h4>\u6982\u8981<\/h4>\n<ul>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u7523\u751f\u3059\u308b\u305f\u3081\u306e\u30d9\u30af\u30bf\u30fc\uff08\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\uff09\u3067\u3059\u3002\u672c\u30af\u30ed\u30fc\u30f3\u3092\u7136\u308b\u3079\u304d\u6761\u4ef6\u4e0b\u3067\u5b9a\u3081\u3089\u308c\u305f\u7d30\u80de\u682a\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\uff08\u642d\u8f09\u907a\u4f1d\u5b50\u306b\u3088\u308b\u7d30\u80de\u6bd2\u6027\u7b49\u306e\u7406\u7531\u304b\u3089\u3001\u4e00\u90e8\u306e\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u304b\u3089\u306f\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u7523\u751f\u3067\u304d\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\uff09<\/li>\n<li>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u57fa\u672c\u696d\u52d9\u3068\u3057\u3066\u3001\u56fd\u5185\u306a\u3089\u3073\u306b\u6d77\u5916\u306b\u304a\u3044\u3066\u7523\u51fa\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u306e\u5bc4\u8a17\u3092\u3046\u3051\u3001\u691c\u5b9a\u5f8c\u4fdd\u5b58\u3057\u54c1\u8cea\u7ba1\u7406\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u306f\u3001\u7814\u7a76\u3067\u5b9f\u969b\u306b\u4f7f\u7528\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306a\u3089\u3073\u306b\u5f53\u958b\u767a\u5ba4\u3067\u72ec\u81ea\u306b\u958b\u767a\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u3001\u4ed6\u306b\u306f\u306a\u3044\u30e6\u30cb\u30fc\u30af\u306a\u3082\u306e\u304c\u591a\u6570\u3042\u308a\u307e\u3059\u3002\n<\/li>\n<li>\u5bc4\u8a17\u8005\u304b\u3089\u63d0\u4f9b\u3055\u308c\u305f\u914d\u5217\u306a\u3089\u3073\u306b\u691c\u5b9a\u306e\u4e00\u74b0\u3068\u3057\u3066\u884c\u3063\u305f\u672b\u7aef\u5869\u57fa\u914d\u5217\u3092\u3001\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u4e0a\u3067\u516c\u958b\u3057\u3066\u3044\u307e\u3059\u3002\u633f\u5165\u907a\u4f1d\u5b50\u306e\u4e21\u7aef\u3092\u30d9\u30af\u30bf\u30fc\u5074\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f7f\u7528\u3057\u3066\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3057\u305f\u7d50\u679c\uff08\u30c9\u30e9\u30d5\u30c8\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\uff09\u3092\u63d0\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u914d\u5217\u306f\u5bc4\u8a17\u6642\u306b\u30af\u30ed\u30fc\u30f3\u306b\u6dfb\u3048\u3089\u308c\u308b\u5bc4\u8a17\u66f8\u985e\u306e\u8a18\u8f09\u5185\u5bb9\u3068\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u5f97\u305f\u3082\u306e\u3067\u3001\u30ea\u30bd\u30fc\u30b9\u306e\u5168\u5869\u57fa\u914d\u5217\u3092\u4fdd\u8a3c\u3059\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\uff08\u30b3\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\uff09\u306f\u3001\u300c\u30a2\u30c7\u30ce\u843d\u3068\u3057\u300d\u306b\u3088\u308a\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306b\u5909\u63db\u53ef\u80fd\u3067\u3042\u308a\uff08\u5236\u9650\u9175\u7d20\u30b5\u30a4\u30c8\u306e\u5236\u7d04\u306b\u3088\u308b\u4e00\u90e8\u306e\u30af\u30ed\u30fc\u30f3\u3092\u9664\u304f\uff09\u3001\u305d\u308c\u306b\u3088\u308a\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u300c\u30a2\u30c7\u30ce\u843d\u3068\u3057\u300d\u306b\u3064\u3044\u3066\u306f\u300c\u5b9f\u9a13\u533b\u5b66 Vol. 21, No. 7 (\uff15\u6708\u53f7) \u30af\u30ed\u30fc\u30ba\u30a2\u30c3\u30d7\u5b9f\u9a13\u6cd5\u3001\u5bfa\u5cf6\u4ed6 pp.931-936\u300d\u3092\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<h4>\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u304b\u3089\u3069\u306e\u69d8\u306b\u3057\u3066\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u5f97\u308b\u306e\u304b\uff1f<\/h4>\n<ul>\n<li>\u5b8c\u5168\u9577\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\n<ul>\n<li>\u76f4\u9396\u5316\u3057\u305f\u30d9\u30af\u30bf\u30fc\u3092HEK293\u7d30\u80de\u306b\u907a\u4f1d\u5b50\u5c0e\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u7523\u751f\u3055\u305b\u308b\u65b9\u6cd5\u3067\u3059\u3002\u4e0b\u8a18\u306e\u30d9\u30af\u30bf\u30fc\u304c\u5229\u7528\u53ef\u80fd\u3067\u3059\u3002<br \/>\n<table>\n<tbody>\n<tr>\n<td>RDB03120&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03120\">pAxcwit<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB03121&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03121\">pAxCAwtit<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus containing CAG promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB05212&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05212\">pAxcwit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB05213&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05213\">pAxCAwtit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB05214&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05214\">pAxCALNLwtit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB05215&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05215\">pAxEFwtit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<\/li>\n<li>\uff23\uff2f\uff33\uff0d\uff34\uff30\uff23\u6cd5\n<ul>\n<li>\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0DNA\u3068\u672b\u7aef\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8907\u5408\u4f53\u3067\u3042\u308b DNA-Terminal Protein Complex (TPC) \u3092\u7528\u3044\u308b\u65b9\u6cd5\u3067\u3059 (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/8577762\/\">Miyake et al., 1996<\/a>)\u3002DNA-TPC \u306f <a href=\"https:\/\/brc.riken.jp\/dna\/RDB01747\" target=\"_blank\">Ad5-dlX (cat# RDB01747)<\/a> \u304b\u3089\u81ea\u4f5c\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h4>\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u6301\u3064\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u3092\u69cb\u7bc9\u3059\u308b<\/h4>\n<ul>\n<li>\u7406\u7814BRC\u30fb\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u69d8\u3005\u306a\u7528\u9014\u306b\u5bfe\u5fdc\u3059\u3079\u304f\u3001\u6570\u591a\u304f\u306e\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u30d9\u30af\u30bf\u30fc\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u3002<a href=\"http:\/\/brc.riken.jp\/dna\/GRP0046e\">Adenovirus Vector [English site]<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"reference_adeno\"><\/a><\/p>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599<\/h3>\n<h4>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u53d6\u6271\u3044<\/h4>\n<ul>\n<li>Ugai H, Yamasaki T, Hirose M, Inabe K, Kujime Y, Terashima M, Liu B, Tang H, Zhao M, Murata T, Kimura M, Pan J, Obata Y, Hamada H, Yokoyama KK. (2005) Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=15882984\">Biochem. Biophys. Res. Commun. 331: 1053-1060.<\/a><\/li>\n<li>Suzuki E, Murata T, Watanabe S, Kujime Y, Hirose M, Pan J, Yamazaki T, Ugai H, Yokoyama KK. (2004) A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=14654922\">Oncol Rep. 11: 173-178.<\/a><\/li>\n<li>H. Ugai, E. Suzuki, K. Inabe, T. Murata, H. Hamada, K. K. Yokoyama (2003) Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells. Biochem. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=12504105\">Biophys. Res. Commun. 300: 448-456.<\/a><\/li>\n<li>\u9d5c\u98fc\u82f1\u4e16\u3001\u5bfa\u5cf6\u7f8e\u4fdd\u3001\u6751\u7530\u6b66\u82f1\u3001\u6a2a\u5c71\u548c\u5c1a\uff1a\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8fc5\u901f\u306a\u7cbe\u88fd\u6cd5 \u300d\u5b9f\u9a13\u533b\u5b66\u3001\u7f8a\u571f\u793e\u300124, 981-985 (2006).<\/li>\n<li>\u5bfa\u5cf6 \u7f8e\u4fdd\u3001\u8fd1\u85e4 \u5c0f\u8cab\u3001\u9418\u30f6\u6c5f \u88d5\u7f8e\u3001\u658e\u85e4 \u6cc9 (2003) \u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u7c21\u4fbf\u306a\u4f5c\u88fd\u6cd5. \u5b9f\u9a13\u533b\u5b66, 21: 931-936.<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u65b0\u8a02\u65b0\u907a\u4f1d\u5b50\u5de5\u5b66\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3001\u6539\u8a02\u7b2c\uff13\u7248\u3001\u6751\u677e\u6b63\u5be6\u3001\u5c71\u672c\u96c5\u7de8\u30011999\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u30d0\u30a4\u30aa\u30de\u30cb\u30e5\u30a2\u30ebUP\u30b7\u30ea\u30fc\u30ba\u3001\u6539\u8a02\u7248\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u5b9f\u9a13\u6cd5\u3001\u7af9\u7e04\u5fe0\u81e3\u7de8\u30011998\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u5225\u518a\u5b9f\u9a13\u533b\u5b66\u3001\u30b6\u30fb\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u30b7\u30ea\u30fc\u30ba\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\uff06\u767a\u73fe\u89e3\u6790\u5b9f\u9a13\u6cd5\u3001\u658e\u85e4\u6cc9\u3001\u83c5\u91ce\u7d14\u592b\u7de8\u96c6\u30011997\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u30d0\u30a4\u30aa\u30de\u30cb\u30e5\u30a2\u30ebUP\u30b7\u30ea\u30fc\u30ba\u3001\u907a\u4f1d\u5b50\u6cbb\u7642\u306e\u57fa\u790e\u6280\u8853\u3001\u5cf6\u7530\u9686\u3001\u658e\u85e4\u6cc9\u3001\u5c0f\u6fa4\u656c\u4e5f\u7de8\u96c6\u30011996\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u30a6\u30a4\u30eb\u30b9\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3001\u6c38\u4e95\u7f8e\u4e4b\u3001\u77f3\u6d5c\u660e\u76e3\u4fee\u3001\u5c0f\u6797\u4fe1\u4e4b\u3001\u6c38\u7530\u606d\u4ecb\u7de8\u96c6\u30011995\u5e74\u3001\u30e1\u30b8\u30ab\u30eb\u30d3\u30e5\u30fc\u793e<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u30d0\u30a4\u30aa\u30de\u30cb\u30e5\u30a2\u30eb\u30b7\u30ea\u30fc\u30ba\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u3068\u767a\u73fe\u30fb\u89e3\u6790\u6cd5\u3001\u6a2a\u7530\u5d07\u3001\u65b0\u4e95\u8ce2\u4e00\u7de8\u96c6\u30011994\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u5b89\u5b9a\u6027<\/h4>\n<ul>\n<li>Ugai, H., Watanabe, S., Suzuki, E., Tsutsui-Nakata, H., Yokoyama, K.K., Murata, T. (2002) Stability of a recombinant adenoviral vector: optimization of conditions for storage, transport and delivery. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=12036457\">Jpn J Cancer Res., 93: 598-603.<\/a><\/li>\n<li>Croyle, M.A., Yu, Q.C., Wilson, J.M. (2000) Development of a rapid method for the PEGylation of adenoviruses with enhanced transduction and improved stability under harsh storage conditions. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10954905\">Hum Gene Ther. 11 (12): 1713-1722.<\/a><\/li>\n<li>Nyberg-Hoffman, C., Aguilar-Cordova, E. (1999) Instability of adenoviral vectors during transport and its implication for clinical studies. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10426323\">Nat Med. 5 (8): 955-957.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u5b8c\u5168\u9577\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\u306b\u3088\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u4f5c\u88fd<\/h4>\n<ul>\n<li><a href=\"\/ja\/manual\/rvd_sopja\">\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb<\/a><\/li>\n<li>Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., and Saito, I. (2006) Possible mechanism of adenovirus generation from a viral genome tagged with nucleotides at its ends. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=16924150\">Microbiol Immunol. 50: 643-654.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>COS-TPC\u6cd5\u306b\u3088\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u4f5c\u88fd<\/h4>\n<ul>\n<li>Miyake S, Makimura M, Kanegae Y, Harada S, Sato Y, Takamori K, Tokuda C, Saito I. (1996) Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=8577762\">Proc. Natl. Acad. Sci. USA 93: 1320-1324.<\/a><\/li>\n<\/ul>\n<\/li>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9(Replication Competent Adenovirus, RCA)<\/h4>\n<ul>\n<li>Suzuki E, Murata T, Watanabe S, Kujime Y, Hirose M, Pan J, Yamazaki T, Ugai H, Yokoyama KK. (2004) A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=14654922\">Oncol Rep. 11: 173-178.<\/a><\/li>\n<li>\u6751\u7530\u6b66\u82f1\u3001\u9d5c\u98fc\u82f1\u4e16\u3001\u9234\u6728\u6075\u7406\u9999\u3001\u6a2a\u5c71\u548c\u5c1a (2001) \u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u5b89\u5168\u6027\u3001\u7d30\u80de\u5de5\u5b66\u3001Vol. 20, No. 9, 1250-1254.<\/li>\n<li>Fallaux FJ, Kranenburg O, Cramer SJ, Houweling A, Van Ormondt H, Hoeben RC, Van Der Eb AJ. (1996). Characterization of 911: a new helper cell line for the titration and propagation of early region 1-deleted adenoviral vectors. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=8788172\">Hum. Gene Ther. 7: 215-222.<\/a><\/li>\n<li>Lochmuller H, Jani A, Huard J, Prescott S, Simoneau M, Massie B, Karpati G, Acsadi G. (1994). Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (delta E1 + delta E3) during multiple passages in 293 cells. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=7711141\">Hum. Gene Ther. 5: 1485-1491.<\/a><\/li>\n<li>Zhu J, Grace M, Casale J, Chang AT, Musco ML, Bordens R, Greenberg R, Schaefer E, Indelicato SR. (1999). Characterization of replication-competent adenovirus isolates from large-scale production of a recombinant adenoviral vector. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10022536\">Hum. Gene Ther. 10: 113-121.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>293\u7d30\u80de\u306eE1\u907a\u4f1d\u5b50\u3092\u7372\u5f97\u3059\u308b\u3053\u3068\u306b\u3088\u308bRCA\u306e\u767a\u751f<\/h4>\n<ul>\n<li>Hehir KM, Armentano D, Cardoza LM, Choquette TL, Berthelette PB, White GA, Couture LA, Everton MB, Keegan J, Martin<br \/>\nJM, Pratt DA, Smith MP, Smith AE, Wadsworth SC (1996). Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=8970968\">J. Virol. 70: 8459-8467.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>RCA\u767a\u751f\u3092\u6291\u5236\u3057\u305f\u5bbf\u4e3b\u7d30\u80de<\/h4>\n<ul>\n<li>Schiedner G, Hertel S, Kochanek S (2000) Efficient transformation of primary human amniocytes by E1 functions of Ad5: generation of new cell lines for adenoviral vector production. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=11044912\">Hum. Gene Ther. 11: 2105-2116. <\/a><\/li>\n<li>Gao GP, Engdahl RK, Wilson JM (2000). A cell line for high-yield production of E1-deleted adenovirus vectors without the emergence of replication-competent virus. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10646652\">Hum. Gene Ther. 11: 213-219.<\/a><\/li>\n<li>Fallaux FJ, Bout A, van der Velde I, van den Wollenberg DJ, Hehir KM, Keegan J, Auger C, Cramer SJ, van Ormondt H, van<br \/>\nder Eb AJ, Valerio D, Hoeben RC (1998). New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=9741429\">Hum. Gene Ther. 9: 1909-1917.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>Cre\u304c\u52d5\u7269\u7d30\u80de\u306b\u4e0e\u3048\u308btoxicity<\/h4>\n<ul>\n<li>Baba, Y., Nakano, M., Yamada, Y., Saito, I., Kanegae, Y. (2005). Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=15965304\">Microbiol. Immunol. 49 (6): 559-570.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u611f\u67d3\u52b9\u7387<\/h4>\n<ul>\n<li>Table in <a href=\"\/en\/viralvecen\/adenoen\">Adenovirus Vector [English site]<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p style=\"text-align: left;\">2025.06.23 (T.M. GRP0046j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066 \u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf \u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599 \u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066 \u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001\u56fd\u5185\u306a\u3089\u3073\u306b\u6d77\u5916\u306b\u304a\u3044\u3066\u7523\u51fa\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u306e\u5bc4\u8a17\u3092\u3046\u3051\u3001\u691c\u5b9a\u5f8c\u4fdd\u5b58\u3057\u54c1\u8cea\u7ba1\u7406\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u7814\u7a76\u3067\u5b9f\u969b\u306b\u4f7f\u7528\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306a\u3089\u3073\u306b\u5f53\u958b\u767a\u5ba4\u3067\u72ec\u81ea\u306b\u958b\u767a\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u3001\u4ed6\u306b\u306f\u306a\u3044\u30e6\u30cb\u30fc\u30af\u306a\u3082\u306e\u304c\u591a\u6570\u3042 [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":6210,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6215","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6215"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6215\/revisions"}],"predecessor-version":[{"id":6219,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6215\/revisions\/6219"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6210"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}