{"id":6215,"date":"2025-06-23T08:24:17","date_gmt":"2025-06-22T23:24:17","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=6215"},"modified":"2025-06-23T08:24:37","modified_gmt":"2025-06-22T23:24:37","slug":"adenoja","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/viralvecja\/adenoja","title":{"rendered":"\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<ul>\n<li><a href=\"#about_adeno\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066<\/a><\/li>\n<li><a href=\"#vector_adeno\">\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf<\/a><\/li>\n<li><a href=\"#reference_adeno\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599<\/a><\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"about_adeno\"><\/a><\/p>\n<p style=\"text-align: right;\"><img loading=\"lazy\" decoding=\"async\" alt=\"aden01\" src=\"https:\/\/dnaconda.riken.jp\/images\/aden01.png\" width=\"73\" height=\"66\" \/><\/p>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066<\/h3>\n<p>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001\u56fd\u5185\u306a\u3089\u3073\u306b\u6d77\u5916\u306b\u304a\u3044\u3066\u7523\u51fa\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u306e\u5bc4\u8a17\u3092\u3046\u3051\u3001\u691c\u5b9a\u5f8c\u4fdd\u5b58\u3057\u54c1\u8cea\u7ba1\u7406\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u7814\u7a76\u3067\u5b9f\u969b\u306b\u4f7f\u7528\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306a\u3089\u3073\u306b\u5f53\u958b\u767a\u5ba4\u3067\u72ec\u81ea\u306b\u958b\u767a\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u3001\u4ed6\u306b\u306f\u306a\u3044\u30e6\u30cb\u30fc\u30af\u306a\u3082\u306e\u304c\u591a\u6570\u3042\u308a\u307e\u3059\u3002<br \/>\n\u7406\u7814BRC\u30fb\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u304a\u3088\u3073\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30d9\u30af\u30bf\u30fc\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u307e\u3059\u3002<br \/>\n\u65b0\u305f\u306b\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u307e\u305f\u306f\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30d9\u30af\u30bf\u30fc\u7b49\u306e\u7814\u7a76\u6750\u6599\u3092\u958b\u767a\u3055\u308c\u305f\u65b9\u306f\u3001\u305c\u3072\u3001\u5f53\u65b9\u306b\u5fa1\u5bc4\u8a17\u4e0b\u3055\u3044\u307e\u3059\u3088\u3046\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<br \/>\n\u305f\u3060\u3057\u3001\u5f53\u30d0\u30f3\u30af\u306f\u3001\u75c5\u539f\u6027\u30a6\u30a4\u30eb\u30b9\u306f\u6271\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\u3042\u3089\u304b\u3058\u3081\u3054\u4e86\u627f\u9858\u3044\u307e\u3059\u3002<\/p>\n<hr \/>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u53d7\u53d6\u3063\u305f\u3089<\/h3>\n<p>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u53d7\u53d6\u3063\u3066\u304b\u3089\u7cbe\u88fd\u3059\u308b\u307e\u3067\u306e\u624b\u9806\u306e\u6982\u8981\u3067\u3059\u3002\u64cd\u4f5c\u624b\u9806\u306f<a href=\"\/ja\/wp-content\/uploads\/manual\/adenovirus3.pdf\" target=\"_blank\">\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300d<\/a>\u306b\u8a18\u8f09\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u5e45\n<ul>\n<li>\u7406\u7814DNA Bank\u304c\u63d0\u4f9b\u3059\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u3068\u3057\u3066\u304a\u9001\u308a\u81f4\u3057\u307e\u3059\u3002\u529b\u4fa1\u306f\u6e2c\u5b9a\u3057\u3066\u3044\u307e\u305b\u3093\u304c\uff0c\u304a\u304a\u3088\u305d10<sup>6-8<\/sup>pfu\/ml\u306e\u6db2\u3092100 \u03bcl \u304a\u9001\u308a\u3044\u305f\u3057\u307e\u3059\u3002\u304a\u53d7\u3051\u53d6\u308a\u5f8c\u306f\u76f4\u3061\u306b\u57f9\u990a\u3092\u958b\u59cb\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u76f4\u3061\u306b\u57f9\u990a\u304c\u3067\u304d\u306a\u3044\u5834\u5408\u306f-80\u2103\u3067\u4fdd\u7ba1\u3057\u3001\u306a\u308b\u3079\u304f\u65e9\u304f\u57f9\u990a\u3057\u3066\u4e0b\u3055\u3044\u3002<\/li>\n<li>\u64cd\u4f5c\u65b9\u6cd5\n<ul>\n<li>10 cm\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u30c7\u30a3\u30c3\u30b7\u30e5\u306b\u57f9\u990a\u3057\u305f293 \u7d30\u80de\uff08\u57f9\u990a\u6db210ml, \u7d049&#215;10<span style=\"vertical-align: super;\">6<\/span>cells\uff09\u3092\u7528\u610f\u3059\u308b\u3002<\/li>\n<li>\u30a6\u30a4\u30eb\u30b9\u6db2\u3092100 \u03bcl\u611f\u67d3\u3055\u305b\u308b\uff08\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300dProtocol C-1 ,3, 4 \u6b21\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u53c2\u7167\uff09<\/li>\n<li>\u7d043\uff5e4 \u65e5\u5f8c\u306b\u7d30\u80de\u304c\u6b7b\u6ec5\u3057\u305f\u3089\u3001\u7d0410ml\u306e\u57f9\u990a\u6db2\u3092\u7d30\u80de\u3054\u3068\uff08\u6b7b\u6ec5\u7d30\u80de\u3054\u3068\uff0950 mL\u30c1\u30e5\u30fc\u30d6\u306b\u56de\u53ce\u3059\u308b\u3002\u3059\u3050\u306b\u4f7f\u308f\u306a\u3044\u5834\u5408\u306f\u7c97\u30a6\u30a4\u30eb\u30b9\u6db2\u3068\u3057\u3066\u305d\u306e\u307e\u307e\uff0d80\u2103\u306b\u4fdd\u5b58\u3059\u308b\u3002\u3059\u3050\u306b\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u5bc6\u9589\u8d85\u97f3\u6ce2\u7834\u7815\u307e\u305f\u306f\u51cd\u7d50\u878d\u89e3\u3057\u3001\u30a6\u30a4\u30eb\u30b9\u3092\u904a\u96e2\u3055\u305b,9,400 g\u300110 \u5206\u30014\u2103\u3067\u9060\u5fc3\u3057\u3066\u4e0a\u6e05\u3092\u56de\u53ce\u3057\u30a6\u30a4\u30eb\u30b9\u6db2\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\uff08\u30a6\u30a4\u30eb\u30b9\u6db2\u7d0410ml\u304c\u5f97\u3089\u308c\u308b\uff09\u3002\u6b8b\u308a\u306f\u3001\u84cb\u304c\u30cd\u30b8\u5f0f\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u5206\u6ce8\u3057\u3066-80\u2103\u306b\u4fdd\u5b58\u3059\u308b\u3002 \u30fb\u3053\u306e\u5f8c\u3001\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300d\u306e\u300c\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u306e\u8d85\u9060\u5fc3\u306b\u3088\u308b\u5927\u91cf\u8abf\u88fd\u300d\u306b\u5f93\u3063\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u7cbe\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8f38\u9001\u306a\u3089\u3073\u306b\u4fdd\u5b58\u6642\u306e\u5b89\u5b9a\u6027\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/12036457\/\">Ugai, H. et al., Jpn. J. Cancer Res., 93, 598-603 (2002).<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u5897\u5e45\u306b\u306fHEK293\u7d30\u80de (RCB1637)\u3092\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/li>\n<li>\u914d\u5217\u3068\u69cb\u9020\u306e\u691c\u67fb\n<ul>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u9650\u3089\u305a\u3001\u7d44\u63db\u3048DNA\u30af\u30ed\u30fc\u30f3\u306f\u7a81\u7136\u5909\u7570\u3092\u8d77\u3053\u3059\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u7d44\u8fbc\u307f\u907a\u4f1d\u5b50\uff08\u30d5\u30a1\u30a4\u30d0\u30fc\u5909\u7570\u4f53\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u30d5\u30a1\u30a4\u30d0\u30fc\u9818\u57df\u3082\uff09\u306e\u6027\u72b6\u3092\u5b9f\u9a13\u306e\u524d\u306b\u3054\u81ea\u8eab\u3067\u8abf\u3079\u3066\u4e0b\u3055\u3044\u3002\u305d\u306e\u65b9\u6cd5\u3068\u3057\u3066\u3001PCR\u3001DNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3042\u308b\u3044\u306f\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u81ea\u5f8b\u5897\u6b96\u30a6\u30a4\u30eb\u30b9\u6df7\u5165\u306e\u691c\u67fb\n<ul>\n<li>\u7406\u7814DNA Bank\u306e\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u305d\u306e\u307b\u3068\u3093\u3069\u304cCOS-TPC\u6cd5\u306b\u3088\u308a\u4f5c\u88fd\u3055\u308c\u3066\u304a\u308a(Miyake S. et al., 1996)\u3001\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9(Replication Competent Adenovirus, RCA)\u304c\u6df7\u5165\u3059\u308b\u6050\u308c\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u5897\u6b96\u904e\u7a0b\u3067\u3082\u5bbf\u4e3b\u3067\u3042\u308bHEK293\u7d30\u80de\u306e\u6301\u3064E1\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u63db\u3048\u306b\u3088\u308a\u7372\u5f97\u3059\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u3057\u3088\u3046\u3059\u308b\u524d\u306b\u306f\u3001RCA\u304c\u5b58\u5728\u3057\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002Suzuki E et al. (2004)\u306b\u305d\u306e\u65b9\u6cd5\u3092\u63b2\u8f09\u3057\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/li>\n<li>\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u691c\u51fa\u306b\u306fHeLa\u7d30\u80de (RCB0007)\u3092\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u7cbe\u88fd\n<ul>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u7c21\u4fbf\u306a\u7cbe\u88fd\u6cd5\u306f\u3001<a href=\"\/ja\/wp-content\/uploads\/manual\/adenovirus3.pdf\" target=\"_blank\">\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e\u300d<\/a>\u306e\u307b\u304b\u3001<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/15882984\/\">Ugai et al., Biochem. Biophys. Res. Commun. 331: 1053-1060 (2005)<\/a>\u3001 \u9d5c\u98fc\u3089\u3001\u5b9f\u9a13\u533b\u5b66\u3001\u7f8a\u571f\u793e\u300124, 981-985 (2006)\u306b\u8a18\u8f09\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<hr \/>\n<p><a name=\"vector_adeno\"><\/a><\/p>\n<h3>\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc<\/h3>\n<h4>\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u4f7f\u7528\u6642\u306e\u6ce8\u610f<\/h4>\n<ul>\n<li>\u7b2c\u4e8c\u7a2e\u4f7f\u7528\u7b49\u3092\u76ee\u7684\u3068\u3057\u305f\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u3067\u3059\u3002<\/li>\n<li>\u5bbf\u4e3b\u306e\u540d\u79f0\u306a\u3089\u3073\u306b\u7d44\u63db\u3048\u6838\u9178\u306e\u540d\u79f0\u306f\u3001\u5404\u30af\u30ed\u30fc\u30f3\u306e\u8a73\u7d30\u60c5\u5831\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\u3002<\/li>\n<li>\u672c\u30d9\u30af\u30bf\u30fc\u306f\u5b9a\u3081\u3089\u308c\u305f\u5b9f\u9a13\u6761\u4ef6\u4e0b\u3067\u5b9a\u3081\u3089\u308c\u305f\u5bbf\u4e3b\u7d30\u80de\u306b\u5c0e\u5165\u3057\u305f\u6642\u306b\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u7523\u751f\u3059\u308b\u69d8\u306b\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u308c\u4ee5\u5916\u306e\u6761\u4ef6\u4e0b\u3067\u30a6\u30a4\u30eb\u30b9\u304c\u7523\u751f\u3055\u308c\u308b\u53ef\u80fd\u6027\u306f\u5426\u5b9a\u3067\u304d\u307e\u305b\u3093\u3002\u5f93\u3044\u307e\u3057\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u4ee5\u5916\u306e\u76ee\u7684\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u4f7f\u7528\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u5341\u5206\u306b\u6ce8\u610f\u3057\u3066\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<h4>\u6982\u8981<\/h4>\n<ul>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u7523\u751f\u3059\u308b\u305f\u3081\u306e\u30d9\u30af\u30bf\u30fc\uff08\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\uff09\u3067\u3059\u3002\u672c\u30af\u30ed\u30fc\u30f3\u3092\u7136\u308b\u3079\u304d\u6761\u4ef6\u4e0b\u3067\u5b9a\u3081\u3089\u308c\u305f\u7d30\u80de\u682a\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\uff08\u642d\u8f09\u907a\u4f1d\u5b50\u306b\u3088\u308b\u7d30\u80de\u6bd2\u6027\u7b49\u306e\u7406\u7531\u304b\u3089\u3001\u4e00\u90e8\u306e\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u304b\u3089\u306f\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092\u7523\u751f\u3067\u304d\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\uff09<\/li>\n<li>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u57fa\u672c\u696d\u52d9\u3068\u3057\u3066\u3001\u56fd\u5185\u306a\u3089\u3073\u306b\u6d77\u5916\u306b\u304a\u3044\u3066\u7523\u51fa\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u306e\u5bc4\u8a17\u3092\u3046\u3051\u3001\u691c\u5b9a\u5f8c\u4fdd\u5b58\u3057\u54c1\u8cea\u7ba1\u7406\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u306f\u3001\u7814\u7a76\u3067\u5b9f\u969b\u306b\u4f7f\u7528\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306a\u3089\u3073\u306b\u5f53\u958b\u767a\u5ba4\u3067\u72ec\u81ea\u306b\u958b\u767a\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u3001\u4ed6\u306b\u306f\u306a\u3044\u30e6\u30cb\u30fc\u30af\u306a\u3082\u306e\u304c\u591a\u6570\u3042\u308a\u307e\u3059\u3002\n<\/li>\n<li>\u5bc4\u8a17\u8005\u304b\u3089\u63d0\u4f9b\u3055\u308c\u305f\u914d\u5217\u306a\u3089\u3073\u306b\u691c\u5b9a\u306e\u4e00\u74b0\u3068\u3057\u3066\u884c\u3063\u305f\u672b\u7aef\u5869\u57fa\u914d\u5217\u3092\u3001\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u4e0a\u3067\u516c\u958b\u3057\u3066\u3044\u307e\u3059\u3002\u633f\u5165\u907a\u4f1d\u5b50\u306e\u4e21\u7aef\u3092\u30d9\u30af\u30bf\u30fc\u5074\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f7f\u7528\u3057\u3066\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3057\u305f\u7d50\u679c\uff08\u30c9\u30e9\u30d5\u30c8\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\uff09\u3092\u63d0\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u914d\u5217\u306f\u5bc4\u8a17\u6642\u306b\u30af\u30ed\u30fc\u30f3\u306b\u6dfb\u3048\u3089\u308c\u308b\u5bc4\u8a17\u66f8\u985e\u306e\u8a18\u8f09\u5185\u5bb9\u3068\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u5f97\u305f\u3082\u306e\u3067\u3001\u30ea\u30bd\u30fc\u30b9\u306e\u5168\u5869\u57fa\u914d\u5217\u3092\u4fdd\u8a3c\u3059\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093<\/li>\n<li>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\uff08\u30b3\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\uff09\u306f\u3001\u300c\u30a2\u30c7\u30ce\u843d\u3068\u3057\u300d\u306b\u3088\u308a\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306b\u5909\u63db\u53ef\u80fd\u3067\u3042\u308a\uff08\u5236\u9650\u9175\u7d20\u30b5\u30a4\u30c8\u306e\u5236\u7d04\u306b\u3088\u308b\u4e00\u90e8\u306e\u30af\u30ed\u30fc\u30f3\u3092\u9664\u304f\uff09\u3001\u305d\u308c\u306b\u3088\u308a\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u300c\u30a2\u30c7\u30ce\u843d\u3068\u3057\u300d\u306b\u3064\u3044\u3066\u306f\u300c\u5b9f\u9a13\u533b\u5b66 Vol. 21, No. 7 (\uff15\u6708\u53f7) \u30af\u30ed\u30fc\u30ba\u30a2\u30c3\u30d7\u5b9f\u9a13\u6cd5\u3001\u5bfa\u5cf6\u4ed6 pp.931-936\u300d\u3092\u3054\u89a7\u4e0b\u3055\u3044\u3002<\/li>\n<\/ul>\n<h4>\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u304b\u3089\u3069\u306e\u69d8\u306b\u3057\u3066\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u5f97\u308b\u306e\u304b\uff1f<\/h4>\n<ul>\n<li>\u5b8c\u5168\u9577\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\n<ul>\n<li>\u76f4\u9396\u5316\u3057\u305f\u30d9\u30af\u30bf\u30fc\u3092HEK293\u7d30\u80de\u306b\u907a\u4f1d\u5b50\u5c0e\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u30a6\u30a4\u30eb\u30b9\u7c92\u5b50\u3092\u7523\u751f\u3055\u305b\u308b\u65b9\u6cd5\u3067\u3059\u3002\u4e0b\u8a18\u306e\u30d9\u30af\u30bf\u30fc\u304c\u5229\u7528\u53ef\u80fd\u3067\u3059\u3002<br \/>\n<table>\n<tbody>\n<tr>\n<td>RDB03120&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03120\">pAxcwit<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB03121&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03121\">pAxCAwtit<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus containing CAG promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB05212&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05212\">pAxcwit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB05213&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05213\">pAxCAwtit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB05214&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05214\">pAxCALNLwtit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<tr>\n<td>RDB05215&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05215\">pAxEFwtit2<\/a><\/td>\n<td>A dual cassette for constructing recombinant adenovirus<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<\/li>\n<li>\uff23\uff2f\uff33\uff0d\uff34\uff30\uff23\u6cd5\n<ul>\n<li>\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0DNA\u3068\u672b\u7aef\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8907\u5408\u4f53\u3067\u3042\u308b DNA-Terminal Protein Complex (TPC) \u3092\u7528\u3044\u308b\u65b9\u6cd5\u3067\u3059 (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/8577762\/\">Miyake et al., 1996<\/a>)\u3002DNA-TPC \u306f <a href=\"https:\/\/brc.riken.jp\/dna\/RDB01747\" target=\"_blank\">Ad5-dlX (cat# RDB01747)<\/a> \u304b\u3089\u81ea\u4f5c\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h4>\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u6301\u3064\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u3092\u69cb\u7bc9\u3059\u308b<\/h4>\n<ul>\n<li>\u7406\u7814BRC\u30fb\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u69d8\u3005\u306a\u7528\u9014\u306b\u5bfe\u5fdc\u3059\u3079\u304f\u3001\u6570\u591a\u304f\u306e\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u30d9\u30af\u30bf\u30fc\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u3002<a href=\"http:\/\/brc.riken.jp\/dna\/GRP0046e\">Adenovirus Vector [English site]<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"reference_adeno\"><\/a><\/p>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599<\/h3>\n<h4>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u53d6\u6271\u3044<\/h4>\n<ul>\n<li>Ugai H, Yamasaki T, Hirose M, Inabe K, Kujime Y, Terashima M, Liu B, Tang H, Zhao M, Murata T, Kimura M, Pan J, Obata Y, Hamada H, Yokoyama KK. (2005) Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=15882984\">Biochem. Biophys. Res. Commun. 331: 1053-1060.<\/a><\/li>\n<li>Suzuki E, Murata T, Watanabe S, Kujime Y, Hirose M, Pan J, Yamazaki T, Ugai H, Yokoyama KK. (2004) A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=14654922\">Oncol Rep. 11: 173-178.<\/a><\/li>\n<li>H. Ugai, E. Suzuki, K. Inabe, T. Murata, H. Hamada, K. K. Yokoyama (2003) Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells. Biochem. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=12504105\">Biophys. Res. Commun. 300: 448-456.<\/a><\/li>\n<li>\u9d5c\u98fc\u82f1\u4e16\u3001\u5bfa\u5cf6\u7f8e\u4fdd\u3001\u6751\u7530\u6b66\u82f1\u3001\u6a2a\u5c71\u548c\u5c1a\uff1a\u300c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8fc5\u901f\u306a\u7cbe\u88fd\u6cd5 \u300d\u5b9f\u9a13\u533b\u5b66\u3001\u7f8a\u571f\u793e\u300124, 981-985 (2006).<\/li>\n<li>\u5bfa\u5cf6 \u7f8e\u4fdd\u3001\u8fd1\u85e4 \u5c0f\u8cab\u3001\u9418\u30f6\u6c5f \u88d5\u7f8e\u3001\u658e\u85e4 \u6cc9 (2003) \u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u7c21\u4fbf\u306a\u4f5c\u88fd\u6cd5. \u5b9f\u9a13\u533b\u5b66, 21: 931-936.<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u65b0\u8a02\u65b0\u907a\u4f1d\u5b50\u5de5\u5b66\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3001\u6539\u8a02\u7b2c\uff13\u7248\u3001\u6751\u677e\u6b63\u5be6\u3001\u5c71\u672c\u96c5\u7de8\u30011999\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u30d0\u30a4\u30aa\u30de\u30cb\u30e5\u30a2\u30ebUP\u30b7\u30ea\u30fc\u30ba\u3001\u6539\u8a02\u7248\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u5b9f\u9a13\u6cd5\u3001\u7af9\u7e04\u5fe0\u81e3\u7de8\u30011998\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u5225\u518a\u5b9f\u9a13\u533b\u5b66\u3001\u30b6\u30fb\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u30b7\u30ea\u30fc\u30ba\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\uff06\u767a\u73fe\u89e3\u6790\u5b9f\u9a13\u6cd5\u3001\u658e\u85e4\u6cc9\u3001\u83c5\u91ce\u7d14\u592b\u7de8\u96c6\u30011997\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u30d0\u30a4\u30aa\u30de\u30cb\u30e5\u30a2\u30ebUP\u30b7\u30ea\u30fc\u30ba\u3001\u907a\u4f1d\u5b50\u6cbb\u7642\u306e\u57fa\u790e\u6280\u8853\u3001\u5cf6\u7530\u9686\u3001\u658e\u85e4\u6cc9\u3001\u5c0f\u6fa4\u656c\u4e5f\u7de8\u96c6\u30011996\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<li>\u30a6\u30a4\u30eb\u30b9\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3001\u6c38\u4e95\u7f8e\u4e4b\u3001\u77f3\u6d5c\u660e\u76e3\u4fee\u3001\u5c0f\u6797\u4fe1\u4e4b\u3001\u6c38\u7530\u606d\u4ecb\u7de8\u96c6\u30011995\u5e74\u3001\u30e1\u30b8\u30ab\u30eb\u30d3\u30e5\u30fc\u793e<\/li>\n<li>\u5b9f\u9a13\u533b\u5b66\u5225\u518a\u3001\u30d0\u30a4\u30aa\u30de\u30cb\u30e5\u30a2\u30eb\u30b7\u30ea\u30fc\u30ba\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u3068\u767a\u73fe\u30fb\u89e3\u6790\u6cd5\u3001\u6a2a\u7530\u5d07\u3001\u65b0\u4e95\u8ce2\u4e00\u7de8\u96c6\u30011994\u5e74\u3001(\u682a)\u7f8a\u571f\u793e<\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u5b89\u5b9a\u6027<\/h4>\n<ul>\n<li>Ugai, H., Watanabe, S., Suzuki, E., Tsutsui-Nakata, H., Yokoyama, K.K., Murata, T. (2002) Stability of a recombinant adenoviral vector: optimization of conditions for storage, transport and delivery. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=12036457\">Jpn J Cancer Res., 93: 598-603.<\/a><\/li>\n<li>Croyle, M.A., Yu, Q.C., Wilson, J.M. (2000) Development of a rapid method for the PEGylation of adenoviruses with enhanced transduction and improved stability under harsh storage conditions. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10954905\">Hum Gene Ther. 11 (12): 1713-1722.<\/a><\/li>\n<li>Nyberg-Hoffman, C., Aguilar-Cordova, E. (1999) Instability of adenoviral vectors during transport and its implication for clinical studies. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10426323\">Nat Med. 5 (8): 955-957.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u5b8c\u5168\u9577\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\u306b\u3088\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u4f5c\u88fd<\/h4>\n<ul>\n<li><a href=\"\/ja\/manual\/rvd_sopja\">\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb<\/a><\/li>\n<li>Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., and Saito, I. (2006) Possible mechanism of adenovirus generation from a viral genome tagged with nucleotides at its ends. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=16924150\">Microbiol Immunol. 50: 643-654.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>COS-TPC\u6cd5\u306b\u3088\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u4f5c\u88fd<\/h4>\n<ul>\n<li>Miyake S, Makimura M, Kanegae Y, Harada S, Sato Y, Takamori K, Tokuda C, Saito I. (1996) Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=8577762\">Proc. Natl. Acad. Sci. USA 93: 1320-1324.<\/a><\/li>\n<\/ul>\n<\/li>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9(Replication Competent Adenovirus, RCA)<\/h4>\n<ul>\n<li>Suzuki E, Murata T, Watanabe S, Kujime Y, Hirose M, Pan J, Yamazaki T, Ugai H, Yokoyama KK. (2004) A simple method for the simultaneous detection of E1A and E1B in adenovirus stocks. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=14654922\">Oncol Rep. 11: 173-178.<\/a><\/li>\n<li>\u6751\u7530\u6b66\u82f1\u3001\u9d5c\u98fc\u82f1\u4e16\u3001\u9234\u6728\u6075\u7406\u9999\u3001\u6a2a\u5c71\u548c\u5c1a (2001) \u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u5b89\u5168\u6027\u3001\u7d30\u80de\u5de5\u5b66\u3001Vol. 20, No. 9, 1250-1254.<\/li>\n<li>Fallaux FJ, Kranenburg O, Cramer SJ, Houweling A, Van Ormondt H, Hoeben RC, Van Der Eb AJ. (1996). Characterization of 911: a new helper cell line for the titration and propagation of early region 1-deleted adenoviral vectors. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=8788172\">Hum. Gene Ther. 7: 215-222.<\/a><\/li>\n<li>Lochmuller H, Jani A, Huard J, Prescott S, Simoneau M, Massie B, Karpati G, Acsadi G. (1994). Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (delta E1 + delta E3) during multiple passages in 293 cells. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=7711141\">Hum. Gene Ther. 5: 1485-1491.<\/a><\/li>\n<li>Zhu J, Grace M, Casale J, Chang AT, Musco ML, Bordens R, Greenberg R, Schaefer E, Indelicato SR. (1999). Characterization of replication-competent adenovirus isolates from large-scale production of a recombinant adenoviral vector. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10022536\">Hum. Gene Ther. 10: 113-121.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>293\u7d30\u80de\u306eE1\u907a\u4f1d\u5b50\u3092\u7372\u5f97\u3059\u308b\u3053\u3068\u306b\u3088\u308bRCA\u306e\u767a\u751f<\/h4>\n<ul>\n<li>Hehir KM, Armentano D, Cardoza LM, Choquette TL, Berthelette PB, White GA, Couture LA, Everton MB, Keegan J, Martin<br \/>\nJM, Pratt DA, Smith MP, Smith AE, Wadsworth SC (1996). Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=8970968\">J. Virol. 70: 8459-8467.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>RCA\u767a\u751f\u3092\u6291\u5236\u3057\u305f\u5bbf\u4e3b\u7d30\u80de<\/h4>\n<ul>\n<li>Schiedner G, Hertel S, Kochanek S (2000) Efficient transformation of primary human amniocytes by E1 functions of Ad5: generation of new cell lines for adenoviral vector production. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=11044912\">Hum. Gene Ther. 11: 2105-2116. <\/a><\/li>\n<li>Gao GP, Engdahl RK, Wilson JM (2000). A cell line for high-yield production of E1-deleted adenovirus vectors without the emergence of replication-competent virus. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10646652\">Hum. Gene Ther. 11: 213-219.<\/a><\/li>\n<li>Fallaux FJ, Bout A, van der Velde I, van den Wollenberg DJ, Hehir KM, Keegan J, Auger C, Cramer SJ, van Ormondt H, van<br \/>\nder Eb AJ, Valerio D, Hoeben RC (1998). New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=9741429\">Hum. Gene Ther. 9: 1909-1917.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>Cre\u304c\u52d5\u7269\u7d30\u80de\u306b\u4e0e\u3048\u308btoxicity<\/h4>\n<ul>\n<li>Baba, Y., Nakano, M., Yamada, Y., Saito, I., Kanegae, Y. (2005). Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=15965304\">Microbiol. Immunol. 49 (6): 559-570.<\/a><\/li>\n<\/ul>\n<p><!-- - - - - - - - - - - - - - - - - - - --><\/p>\n<h4>\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u611f\u67d3\u52b9\u7387<\/h4>\n<ul>\n<li>Table in <a href=\"\/en\/viralvecen\/adenoen\">Adenovirus Vector [English site]<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p style=\"text-align: left;\">2025.06.23 (T.M. GRP0046j)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066 \u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf \u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599 \u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3057\u3066 \u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001\u56fd\u5185\u306a\u3089\u3073\u306b\u6d77\u5916\u306b\u304a\u3044\u3066\u7523\u51fa\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u306e\u5bc4\u8a17\u3092\u3046\u3051\u3001\u691c\u5b9a\u5f8c\u4fdd\u5b58\u3057\u54c1\u8cea\u7ba1\u7406\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u7814\u7a76\u3067\u5b9f\u969b\u306b\u4f7f\u7528\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306a\u3089\u3073\u306b\u5f53\u958b\u767a\u5ba4\u3067\u72ec\u81ea\u306b\u958b\u767a\u3057\u305f\u30af\u30ed\u30fc\u30f3\u3092\u53ce\u96c6\u3057\u3066\u304a\u308a\u3001\u4ed6\u306b\u306f\u306a\u3044\u30e6\u30cb\u30fc\u30af\u306a\u3082\u306e\u304c\u591a\u6570\u3042 [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":6210,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6215","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6215"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6215\/revisions"}],"predecessor-version":[{"id":6219,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6215\/revisions\/6219"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6210"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}