::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
\nRIKEN BioResource Research Center DNA Bank Mail News No. 129 (2023.06.05)
\n[hp] https:\/\/dna.brc.riken.jp\/ja\/news_archives\/mail
\n————————————————————————
\n\u3053\u306e\u30e1\u30fc\u30eb\u30cb\u30e5\u30fc\u30b9\u306f\u3001\u7406\u7814BRC\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\uff08DNA Bank\uff09\u3092\u3054\u5229\u7528\u3044\u305f\u3060\u3044\u305f\u65b9\u3001\u306a\u3089\u3073\u306b\u30e1\u30fc\u30eb\u30cb\u30e5\u30fc\u30b9\u914d\u4fe1\u306e\u304a\u7533\u3057\u8fbc\u307f\u3092\u3044\u305f\u3060\u3044\u305f\u65b9\u306b\u304a\u9001\u308a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n
::::: CONTENTS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::
\n1. \u6ce8\u76ee\u30ea\u30bd\u30fc\u30b9
\n2. \u5b66\u4f1a\u5c55\u793a\u60c5\u5831
\n::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
\n1. \u6ce8\u76ee\u30ea\u30bd\u30fc\u30b9
\n::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::<\/p>\n
\u69d8\u3005\u306a\u751f\u7269\u306b\u7531\u6765\u3059\u308b\u9ad8\u8f1d\u5ea6\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3084\u767a\u5149\u30bf\u30f3\u30d1\u30af\u8cea<\/p>\n
\u81ea\u7136\u754c\u306b\u306f\u3001\u30db\u30bf\u30eb\u3084\u6d77\u6d0b\u751f\u7269\u306e\u3088\u3046\u306b\u81ea\u3089\u767a\u5149\u3059\u308b\u751f\u7269\u304c\u305f\u304f\u3055\u3093\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u3002\u767a\u5149\u73fe\u8c61\u306f\u3001\u57fa\u8cea\u306e\u9178\u5316\u53cd\u5fdc\u306b\u3088\u308b\u3082\u306e\u3067\u3059\u304c\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30fb\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4\u306b\u4ee3\u8868\u3055\u308c\u308b\u3088\u3046\u306b\u3001\u767a\u5149\u3092\u6d3b\u7528\u3057\u305f\u7814\u7a76\u624b\u6cd5\u304c\u3001\u5e83\u304f\u666e\u53ca\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u30ec\u30dd\u30fc\u30bf\u30fc\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u308b\u767a\u5149\u9175\u7d20\u3068\u3057\u3066\u3001\u5317\u30a2\u30e1\u30ea\u30ab\u7523\u30db\u30bf\u30eb(Photinus pyralis)\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u304c\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u4ed6\u306b\u3082\u8fd1\u5e74\u307e\u3067\u306b\u3001\u30ab\u30a4\u30a2\u30b7\u985e(\u30d7\u30e9\u30f3\u30af\u30c8\u30f3)\u3084\u30a8\u30d3\u306a\u3069\u306e\u6d77\u6d0b\u751f\u7269\u306b\u7531\u6765\u3059\u308b\u69d8\u3005\u306a\u767a\u5149\u9175\u7d20\u304c\u898b\u51fa\u3055\u308c\u3066\u304a\u308a\u3001\u5206\u6ccc\u578b\u306a\u306e\u3067\u7d30\u80de\u5916\u3067\u4f7f\u7528\u3067\u304d\u308b\u3082\u306e\u3084\u3001\u4eba\u5de5\u7684\u306b\u8a2d\u8a08\u3055\u308c\u305f\u767a\u5149\u9175\u7d20\u306e\u958b\u767a\u306a\u3069\u3082\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u4e2d\u306b\u306f\u3001\u30db\u30bf\u30eb\u7531\u6765\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u6bd4\u8f03\u3057\u3066\u3001\u5206\u5b50\u91cf\u304c\u5c0f\u3055\u3044\u305f\u3081\u3001\u767a\u5149\u9175\u7d20\u306b\u7d50\u5408\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u306b\u304f\u3044\u7279\u5fb4\u3092\u6709\u3057\u3066\u3044\u308b\u3082\u306e\u3082\u3042\u308a\u3001\u751f\u7d30\u80de\u5185\u3067\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u52d5\u614b\u306a\u3069\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u691c\u51fa\u3067\u304d\u308b\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u7814\u7a76\u306a\u3069\u306b\u6d3b\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n
JNC\u682a\u5f0f\u4f1a\u793e(\u4ee5\u4e0b\u3001JNC\u793e) \u306f\u3001\u751f\u7269\u767a\u5149\u306b\u95a2\u3059\u308b\u9577\u5e74\u306b\u308f\u305f\u308b\u7814\u7a76\u6210\u679c\u3068\u3057\u3066\u3001\u7d30\u80de\u304b\u3089\u306e\u30a4\u30f3\u30b9\u30ea\u30f3\u5206\u6ccc\u3092\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u65b9\u6cd5\u306b\u3088\u3063\u3066\u89b3\u5bdf\u3057\u3001\u7d30\u80de\u5168\u4f53\u306b\u304a\u3051\u308b\u30a8\u30ad\u30bd\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u90e8\u4f4d\u306e\u5c40\u5728\u3092\u7279\u5b9a\u3057\u3046\u308b\u3053\u3068\u3001\u7d30\u80de\u304b\u3089\u306e\u30a4\u30f3\u30b9\u30ea\u30f3\u5206\u6ccc\u91cf\u3092\u5b9a\u91cf\u3057\u3046\u308b\u3053\u3068\u306a\u3069\u3092\u898b\u3044\u3060\u3057\u3066\u3044\u307e\u3059(Suzuki, T. et al. Anal. Bichem., 2011., Suzuki, T. et al. Plos One., 2011)\u3002JNC\u793e\u3067\u306f\u3001\u7814\u7a76\u904e\u7a0b\u3067\u958b\u767a\u3057\u305f\u751f\u7269\u767a\u5149\u9175\u7d20\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u8ca9\u58f2\u3057\u3066\u304a\u308a\u307e\u3057\u305f\u304c\u3001\u3053\u306e\u5ea6\u3001\u30ab\u30a4\u30a2\u30b7\u985e\u306e\u30ac\u30a6\u30b7\u30a2\u30fb\u30d7\u30ea\u30f3\u30bb\u30c3\u30d7\u30b9(Gaussia princeps)\u7531\u6765\u3084\u30d2\u30aa\u30c9\u30b7\u30a8\u30d3(Oplophorus gracilirostris)\u7531\u6765\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3084\u30aa\u30ef\u30f3\u30af\u30e9\u30b2(Aequorea victoria)\u7531\u6765\u306e\u30a4\u30af\u30aa\u30ea\u30f3\u306a\u3069\u306e\u767a\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u542b\u308027\u7a2e\u985e\u306e\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3092\u3054\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u5f53\u5ba4\u304c\u5f15\u304d\u7d99\u3044\u3067\u3001\u3054\u63d0\u4f9b\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n
\u307e\u305f\u3001\u7523\u696d\u6280\u8853\u7dcf\u5408\u7814\u7a76\u6240\u306e\u91d1 \u8aa0\u57f9\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u30ab\u30a4\u30a2\u30b7\u985e\u7531\u6765\u306e\u767a\u5149\u9175\u7d20\u3092\u5143\u306b\u3001\u6700\u9069\u306a\u30a2\u30df\u30ce\u9178\u914d\u5217\u3092\u4eba\u70ba\u7684\u306b\u6539\u826f\u30fb\u6539\u5584\u3059\u308b\u3053\u3068\u3067\u3001\u5143\u3005\u306e\u767a\u5149\u9175\u7d20\u3088\u308a\u6700\u5927\u3067100\u500d\u306e\u9ad8\u8f1d\u5ea6\u3067\u3042\u308a\u306a\u304c\u3089\u3001\u767a\u5149\u6301\u7d9a\u6027\u306e\u3042\u308b\u4eba\u5de5\u751f\u7269\u767a\u5149\u9175\u7d20(Artificial luciferase; ALuc)\u3092\u958b\u767a\u3057\u307e\u3057\u305f(Kim, S.B. et al. Bioconjug. Chem., 2013)\u3002\u3053\u306e\u5ea6\u30012\u7a2e\u985e\u306eALuc\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u3054\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002<\/p>\n
\u305d\u306e\u4ed6\u3001\u54fa\u4e73\u52d5\u7269\u306e\u7d44\u7e54\u3067\u5149\u306e\u900f\u904e\u6027\u306e\u9ad8\u3044\u8d64\u8272\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc(Akaluc)\u3001\u74b0\u5883\u8981\u56e0\u306b\u4f5c\u7528\u3055\u308c\u306b\u304f\u3044\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc(PmatLuc1\u3084PsagRE1Luc\u306a\u3069)\u306a\u3069\u3082\u3054\u63d0\u4f9b\u3067\u304d\u307e\u3059\u3002\u7814\u7a76\u7528\u9014\u306b\u5408\u308f\u305b\u3066\u304a\u9078\u3073\u304f\u3060\u3055\u3044\u3002\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n
\u25bc \u8ad6\u6587
\nInouye, S. et al. Proc. Natl. Acad. Sci. U S A. 82 (10): 3154-3158, 1985. PMID 3858813
\nInouye, S. et al. Protein Expr. Purif. 109: 47-54, 2015. PMID 25665506
\nInouye, S. et al. Protein Expr. Purif. 52: 66-73, 2007. PMID 16997571
\nInouye, S. FEBS Lett. 347 (2-3): 163-168, 1994. PMID 8033996
\nInouye, S. et al. FEBS Lett. 315 (3): 343-346, 1993. PMID 8422928
\nInouye, S. J. Biochem. 143 (5):711-717, 2008. PMID 18296715
\nInouye, S. et al. FEBS Lett. 341 (2-3): 277-280, 1994. PMID 8137953
\nInouye, S. et al. FEBS Lett. 481 (1): 19-25, 2000. PMID 10984608
\nInouye, S. et al. Biochem. Biophys. Res. Commun. 445 (1): 157-162, 2014. PMID 24491536
\nInouye, S. et al. Biochem. Biosci. Biotechnol. 75 (3): 568-571, 2011. PMID 21389603
\nInouye, S. et al. Biochem. Biophys. Res. Commun. 376 (3): 448-453, 2008. PMID 18789309
\nSuzuki, T. et al. Anal. Bichem. 415 (2): 182-189, 2011. PMID 21477579
\nSuzuki, T. et al. Plos One. 6 (9): e25243, 2011. PMID 21969874
\nInouye, S. et al. Protein Expr. Purif. 128: 93-100, 2016. PMID 27506135
\nInouye, S. & Sahara, Y. Protein Expr. Purif. 53 (2): 384-389, 2007. PMID 17275329
\nKim, S.B. et al. Bioconjug. Chem. 24 (12): 2067-2075, 2013. PMID 24237362
\nKim, S.B. et al. ACS Comb. Sci. 19 (9): 594-599, 2017. PMID 28742969
\n\u4eba\u70ba\u7684\u306b\u8a2d\u8a08\u30fb\u958b\u767a\u3057\u305f\u751f\u7269\u767a\u5149\u9175\u7d20(ALuc)
\n(\u7523\u696d\u6280\u8853\u7dcf\u5408\u7814\u7a76\u6240\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9 2013.11.26)
\n\u4eba\u5de5\u751f\u7269\u767a\u5149\u9175\u7d20(ALucR)\u306b\u9078\u629e\u7684\u306b\u767a\u5149\u3059\u308b\u57fa\u8cea\u3092\u958b\u767a
\n(\u7523\u696d\u6280\u8853\u7dcf\u5408\u7814\u7a76\u6240\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9 2017.12.22)<\/p>\n
\u25bc \u30ea\u30bd\u30fc\u30b9\u60c5\u5831
\nJNC CORPORATION’s Resource
\nURL: https:\/\/dnaconda.riken.jp\/search\/depositor\/dep104979.html
\nSung-Bae Kim’s Resource
\nURL: https:\/\/dnaconda.riken.jp\/search\/depositor\/dep100041.html
\n\u9ad8\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc
\nURL: https:\/\/dna.brc.riken.jp\/ja\/gene_analysis\/inf0039j<\/p>\n
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\n2. \u5b66\u4f1a\u5c55\u793a\u60c5\u5831
\n::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
\n\u4e0b\u8a18\u306e\u65e5\u7a0b\u3067\u3001\u30d6\u30fc\u30b9\u3092\u51fa\u5c55\u3044\u305f\u3057\u307e\u3059\u3002\u5b66\u4f1a\u306b\u3054\u53c2\u52a0\u306e\u6298\u306b\u306f\u3001\u30d6\u30fc\u30b9\u306b\u304a\u7acb\u3061\u5bc4\u308a\u3044\u305f\u3060\u304d\u307e\u3059\u3088\u3046\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002\u304a\u63a2\u3057\u306e\u907a\u4f1d\u5b50\u6750\u6599\u304c\u3054\u3056\u3044\u307e\u3057\u305f\u3089\u3069\u3046\u305e\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002\u6700\u9069\u306a\u907a\u4f1d\u5b50\u6750\u6599\u3092\u3054\u63d0\u6848\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n
\u65e5\u672c\u30b2\u30ce\u30e0\u7de8\u96c6\u5b66\u4f1a \u7b2c8\u56de\u5927\u4f1a
\n\u4f1a\u3000\u671f\uff1a2023\u5e746\u67086\u65e5(\u706b) \uff5e 6\u67088\u65e5(\u6728)
\n\u4f1a\u3000\u5834\uff1a\u30bf\u30ef\u30fc\u30db\u30fc\u30eb\u8239\u58001F\u3000\u5c55\u793a\u30db\u30fc\u30eb
\nURL: https:\/\/www2.aeplan.co.jp\/ge2023\/<\/p>\n
\u7b2c50\u56de\u65e5\u672c\u6bd2\u6027\u5b66\u4f1a\u5b66\u8853\u5e74\u4f1a
\n\u4f1a\u3000\u671f\uff1a2023\u5e746\u670819\u65e5(\u6708) \uff5e 6\u670821\u65e5(\u6c34)
\n\u4f1a\u3000\u5834\uff1a\u30d1\u30b7\u30d5\u30a3\u30b3\u6a2a\u6d5c \u4f1a\u8b70\u30bb\u30f3\u30bf\u30fc3F\u3000NBRP\u30d6\u30fc\u30b9\u3000No.86
\nURL: https:\/\/jsot2023.jp\/index.html<\/p>\n
\u7b2c75\u56de\u65e5\u672c\u7d30\u80de\u751f\u7269\u5b66\u4f1a\u5927\u4f1a
\n\u4f1a\u3000\u671f\uff1a2023\u5e746\u670828\u65e5(\u6c34) \uff5e 6\u670830\u65e5(\u91d1)
\n\u4f1a\u3000\u5834\uff1a\u5948\u826f\u770c\u30b3\u30f3\u30d9\u30f3\u30b7\u30e7\u30f3\u30bb\u30f3\u30bf\u30fc1F\u3000\u30b3\u30f3\u30d9\u30f3\u30b7\u30e7\u30f3\u30db\u30fc\u30eb
\nURL: https:\/\/synonis.com\/jscb2023<\/p>\n
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\n\u25cb \u672c\u30e1\u30fc\u30eb\u30cb\u30e5\u30fc\u30b9\u306e\u914d\u4fe1\u505c\u6b62\u3084\u30a2\u30c9\u30ec\u30b9\u306e\u5909\u66f4\u306f\u3001DNA Bank (dna_sec.brc@riken.jp)\u307e\u3067\u3054\u9023\u7d61\u4e0b\u3055\u3044\u3002
\n\u25cb \u914d\u4fe1\u6e08\u307f\u306e\u30e1\u30fc\u30eb\u30cb\u30e5\u30fc\u30b9\u306f\u4e0b\u8a18\u306eweb\u30b5\u30a4\u30c8\u306b\u63b2\u8f09\u3057\u3066\u304a\u308a\u307e\u3059\u3002
\nURL: https:\/\/dna.brc.riken.jp\/ja\/news_archives\/mail
\n————————————————————————
\n\u914d\u4fe1
\n\u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u7814\u7a76\u30bb\u30f3\u30bf\u30fc
\n\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4 (DNA Bank)
\n[e-mail] dna_sec.brc@riken.jp
\n[hp] https:\/\/dna.brc.riken.jp\/ja\/
\n::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
\n2023.06.05.<\/p>\n","protected":false},"excerpt":{"rendered":"
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RIKEN BioResource Research Center DNA Bank Mail News No. 129 (2023.06.05) [hp] https:\/\/dna.brc.riken.jp\/ja\/news_archives\/mail & […]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":4816,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-5616","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5616","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=5616"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5616\/revisions"}],"predecessor-version":[{"id":5620,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5616\/revisions\/5620"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4816"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=5616"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}