\u25a0\u3000\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u30d0\u30f3\u30af\u4e8b\u696d\u3067\u306f\u672a\u516c\u958b\u5206\u3092\u542b\u3081\u3066\u3001\u3053\u308c\u307e\u3067\u306b584\u901a\u308a\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u3053\u308c\u307e\u3067\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u901a\u3058\u3066\u7d4c\u9a13\u3057\u305f\u7d30\u80de\u306e\u53d6\u308a\u6271\u3044\u306b\u3064\u3044\u3066\u8ff0\u3079\u307e\u3059\u3002 (Mail News 2006.02.24 \u63b2\u8f09\u8a18\u4e8b\u3088\u308a)<\/p>\n","protected":false},"excerpt":{"rendered":" Luciferase assay\u306e\u30b3\u30c4 \u25a0\u3000\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u30d0\u30f3\u30af\u4e8b\u696d\u3067\u306f\u672a\u516c\u958b\u5206\u3092\u542b\u3081\u3066\u3001\u3053\u308c\u307e\u3067\u306b584\u901a\u308a\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u3053\u308c\u307e\u3067\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u901a\u3058\u3066\u7d4c\u9a13\u3057\u305f\u7d30\u80de\u306e\u53d6\u308a\u6271\u3044\u306b\u3064\u3044\u3066\u8ff0\u3079\u307e\u3059\u3002 \u25a0\u3000\u4f7f\u7528\u3057\u305f\u3059\u3079\u3066\u306e\u7d30\u80de\u306b\u3044\u3048\u308b\u3053\u3068\u3067\u3059\u304c\u3001transfection\u306e\u5f53\u65e5\u306b\u5bc6\u5ea6\u304c70\uff5e90%\u306b\u306a\u308b\u3088\u3046\u7d30\u80de\u3092\u64ad\u7a2e\u3059\u308b\u3053\u3068\u3067\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u3092\u4e0a\u3052\u308b\u3053\u3068\u304c\u51fa\u6765\u307e\u3057\u305f\u3002\u307e\u305ftransfect […]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":296,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-4504","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=4504"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4504\/revisions"}],"predecessor-version":[{"id":4868,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4504\/revisions\/4868"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/296"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=4504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n\u25a0\u3000\u4f7f\u7528\u3057\u305f\u3059\u3079\u3066\u306e\u7d30\u80de\u306b\u3044\u3048\u308b\u3053\u3068\u3067\u3059\u304c\u3001transfection\u306e\u5f53\u65e5\u306b\u5bc6\u5ea6\u304c70\uff5e90%\u306b\u306a\u308b\u3088\u3046\u7d30\u80de\u3092\u64ad\u7a2e\u3059\u308b\u3053\u3068\u3067\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u3092\u4e0a\u3052\u308b\u3053\u3068\u304c\u51fa\u6765\u307e\u3057\u305f\u3002\u307e\u305ftransfection\u5f8c\u306e\u57f9\u5730\u4ea4\u63db\u306f\u3001\u6975\u529b\u7d30\u80de\u306b\u30b9\u30c8\u30ec\u30b9\u3092\u4e0e\u3048\u306a\u3044\u3088\u3046\u306b\u614e\u91cd\u306b\u884c\u3046\u3068\u3088\u3044\u3067\u3057\u3087\u3046\u3002PBS(-)\u3067\u6d17\u6d44\u3092\u884c\u3046\u3088\u3046\u6307\u793a\u3059\u308b\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3082\u3042\u308a\u307e\u3059\u304c\u3001\u3053\u308c\u3092\u884c\u3046\u3053\u3068\u3067\u7d30\u80de\u304c\u30c0\u30e1\u30fc\u30b8\u3092\u53d7\u3051\u3066\u6d6e\u904a\u3057\u3066\u3057\u307e\u3044\u3001\u30a2\u30c3\u30bb\u30a4\u3092\u3057\u3088\u3046\u3068\u3057\u305f\u6642\uff08\u3053\u3053\u3067\u306ftransfection\u3088\u308a48hr\u5f8c\uff09\u306b\u306f\u7d30\u80de\u304c\u6b7b\u6ec5\u3057\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u3082\u3042\u308b\u306e\u3067\u6ce8\u610f\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u7d30\u80de\u304c\u6d6e\u904a\u3057\u3066\u3057\u307e\u3063\u3066\u3082\u30a2\u30c3\u30bb\u30a4\u3092\u3057\u3066\u307f\u308b\u3068\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u6d3b\u6027\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u6700\u9069\u306a\u6761\u4ef6\u3092\u691c\u8a0e\u3057\u305f\u4e0a\u3067\u63a5\u7740\u7d30\u80de\u304c\u6e1b\u3063\u3066\u3057\u307e\u3063\u3066\u3082\u8ae6\u3081\u305a\u306b\u30a2\u30c3\u30bb\u30a4\u3057\u3066\u307f\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002
\n\u25a0\u3000\u5404\u7d30\u80de\u3054\u3068\u306e\u7d99\u4ee3\u6642\u3001\u30a2\u30c3\u30bb\u30a4\u6642\u306e\u6ce8\u610f\u70b9\u306f\u4e0b\u8a18\u306e\u901a\u308a\u3067\u3059\u3002\u307e\u305f\u3001\u5206\u5316\u5b9f\u9a13\u3092\u884c\u3063\u305f\u5834\u5408\u306f\u57f9\u990a\u6761\u4ef6\u3068\u53c2\u8003\u6587\u732e\u3092\u4ed8\u8a18\u3057\u307e\u3057\u305f\u3002
\n\u25a0\u3000HeLa<\/span>: \u6bd4\u8f03\u7684\u5bb9\u6613\u306b\u57f9\u990a\u3067\u304d\u305f\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: [1]+20% FCS: Transfection\u3088\u308a6hr\u5f8c\u3001\u901a\u5e38\u57f9\u5730\u306bmedium change \uff08\u4ee5\u4e0bMC\uff09\u3057\u3001\u3055\u3089\u306b30min\u5f8cMC (DMEM + 20% FCS)\u3002[2]+TNF\u03b1: Transfection\u3088\u308a6hr\u5f8cMC (DMEM + TNF\u03b1 1ug\/mL)\u3002
\n\u53c2\u8003\u6587\u732e: [1]M. Kurabayashi. et al. (1995): Sequences of the 5′-flanking region of the human helix-loop-helix protein-encoding Id2A gene, and promoter activity regulated by serum and c-Jun\/AP-1. Gene 156, 311-312. [2]C. Y. Ito et al. (1994): Three NF-\u03baB sites in the I\u03baB-\u03b1 promoter are required for induction of gene expression by TNF\u03b1. Nucleic Acids Res. 22, 3787-3792.
\n\u25a0\u3000HepG2<\/span>: \u5265\u304c\u308c\u306b\u304f\u304f\u96c6\u584a\u3092\u5f62\u6210\u3057\u3084\u3059\u3044\u7d30\u80de\u306a\u306e\u3067\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u3092\u884c\u3063\u305f\u3042\u3068\u306b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u3088\u304f\u884c\u3044\u3001\u7d30\u80de\u3092\u5206\u6563\u3055\u305b\u3066\u304b\u3089\u64ad\u7a2e\u3059\u308b\u3068\u3088\u3044\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: [1]+dexamethasone: Transfection\u3088\u308a6hr\u5f8cMC (MEM + dexamethasone 1 uM)\u3002[2]heat shock: Transfection\u3088\u308a1hr\u5f8cmedium addition\u3002\u3055\u3089\u306b5hr\u5f8cMC +42\u2103 incubation\u3002[3]+CoCl2: Transfection\u3088\u308a6hr\u5f8cMC (MEM + CoCl2 150 uM)\u3002Transfection\u3088\u308a24hr\u5f8cAssay\u3002
\n\u53c2\u8003\u6587\u732e: [1]H. Nakabayashi. et al. (1989): Transcriptional regulation of \u03b1-fetoprotein expression by dexamethasone in human hepatoma cells. J. Biol. Chem. 264, 266-271. [2]R. C. Smith et al. (2002): Spatial and temporal control of transgene expression through ultrasound-mediated induction of the heat shock protein 70B promoter in vivo. Human Gene Ther. 13:697-706. [3]E. Minet et al. (1999): HIF1A gene transcription is dependent on a core promoter sequence encompassing activating and inhibiting sequences located upstream from the transcription initiation site and cis elements located within the 5′ URT. Biochem. Biophys. Res. Commun. 261, 534-540.
\n\u25a0\u3000HEK293<\/span>: \u7d30\u80de\u57f9\u990a\u7528\u306e\u30ce\u30f3\u30b3\u30fc\u30c8\u30c7\u30a3\u30c3\u30b7\u30e5\u3067\u3082\u57f9\u990a\u53ef\u80fd\u3067\u3042\u308b\u304c\u3001\u63a5\u7740\u529b\u304c\u5f31\u304f\u5265\u304c\u308c\u3084\u3059\u3044\u3002\u3069\u3046\u3057\u3066\u3082\u5265\u304c\u308c\u3066\u3057\u307e\u3046\u5834\u5408\u306b\u306f\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u4f7f\u7528\u3059\u308b\u3068\u3088\u3044\u3002
\n\u25a0\u3000Saos-2<\/span>: \u9577\u671f\u7d99\u4ee3\u3059\u308b\u3068\u4e0d\u898f\u5247\u306a\u5f62\u614b\u3092\u793a\u3059\u7d30\u80de\u304c\u73fe\u308c\u308b\u5834\u5408\u304c\u3042\u3063\u305f\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: serum starvation: Transfection\u3088\u308a6hr\u5f8cMC (McCoy’s 5A + 0.5% FCS)\u3002Transfection\u3088\u308a24hr\u5f8cAssay\u3002
\n\u53c2\u8003\u6587\u732e: A. Zaika. et al. (2001): Oncogenes induce and activate endogenous p73 protein. J. Biol. Chem. 276, 11310-11316.
\n\u25a0\u3000MCF-7<\/span>: \u9577\u671f\u7d99\u4ee3\u3059\u308b\u3068\u6027\u8cea\u304c\u5909\u5316\u3059\u308b\u305f\u3081\u3001\u30a2\u30c3\u30bb\u30a4\u306b\u306f\u306a\u308b\u3079\u304f\u7d99\u4ee3\u6570\u306e\u5c11\u306a\u3044\u7d30\u80de\u3092\u4f7f\u7528\u3059\u308b\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: +progesterone: Transfection\u3088\u308a6hr\u5f8cMC (DMEM + progesterone 10 ug\/mL)\u3002
\n\u53c2\u8003\u6587\u732e: K. Nishida et al. (1997): Indentification of regulatory elements of human \u03b16 integrin subunit gene. Biochem. Biophys. Res. Commun. 241, 258-263.
\n\u25a0\u3000B16<\/span>: pH\u304c\u4e0b\u304c\u308a\u3084\u3059\u3044\u306e\u3067\u3001\u7d30\u80de\u5bc6\u5ea6\u306e\u9ad8\u3044\u72b6\u614b\u304c\u7d9a\u304b\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u3002
\n\u25a0\u3000NIH3T3<\/span>: \u98fd\u548c\u5bc6\u5ea6\u306b\u306a\u308b\u3068\u63a5\u89e6\u963b\u5bb3\u3092\u8d77\u3053\u3059\u305f\u3081\u3001\u7d99\u4ee3\u306f\u7d30\u80de\u540c\u58eb\u306b\u9699\u9593\u304c\u3042\u308b\u3046\u3061\u306b\u884c\u3046\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: serum starvation: Transfection\u3088\u308a6hr\u5f8cMC (DMEM + 0.5% FCS)\u3002Transfection\u3088\u308a24hr\u5f8cMC (DMEM + 10% FCS)\u3002
\n\u53c2\u8003\u6587\u732e: J. Schaley et al. (2000): Induction of the cellular E2F-1 promoter by the adenovirus E4-6\/7 protein. J. Virol. 74, 2084-2093.
\n\u25a0\u3000WI38<\/span>: \u6709\u9650\u306e\u5bff\u547d\u3057\u304b\u3082\u305f\u306a\u3044\u306e\u3067\u3001\u98fd\u548c\u5bc6\u5ea6\u306b\u306a\u3089\u306a\u304f\u306a\u3063\u305f\u3089\u5897\u6b96\u671f\u304c\u7d42\u4e86\u3057\u305f\u3068\u5224\u65ad\u3057\u3001\u30a2\u30c3\u30bb\u30a4\u306b\u306f\u4f7f\u7528\u3057\u306a\u3044\u3002
\n\u25a0\u3000C2C12<\/span>: \u6bd4\u8f03\u7684\u5bb9\u6613\u306b\u57f9\u990a\u3067\u304d\u305f\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: [1]+DM: Transfection\u3088\u308a6hr\u5f8cMC (DMEM + FCS-, insulin 10 ug\/mL, transferrin 10 ug\/mL)\u3002[2]+2% horse serum: Transfection\u3088\u308a6hr\u5f8cMC (DMEM + horse serum 2%)\u3002
\n\u53c2\u8003\u6587\u732e: [1]H.-S. Kwon et al. (2004): Protein kinase B-\u03b1 inhibits human pyruvate dehydrogenase kinase-4 gene induction by dexamethasone through inactivation of FOXO transcription factors. Diabetes 53, 899-910. [2]M. M. Katabi et al. (1999): Hexokinase type II: a novel tumor-specific promoter for gene-targeted therapy differentially expressed and regulated in human cancer cells. Hum. Gene Ther. 10, 155-164.
\n\u25a0\u3000CHO-K1<\/span>: \u6bd4\u8f03\u7684\u5bb9\u6613\u306b\u57f9\u990a\u3067\u304d\u305f\u3002
\n\u25a0\u3000COS-7<\/span>: \u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u3092\u3057\u3059\u304e\u308b\u3068\u4ed8\u7740\u7387\u304c\u4f4e\u4e0b\u3059\u308b\u306e\u3067\u6ce8\u610f\u3002
\n\u25a0\u3000F9<\/span>: \u9577\u671f\u9593\u306e\u57f9\u990a\u3067\u6027\u8cea\u304c\u5909\u5316\u3059\u308b\u3053\u3068\u304c\u591a\u304b\u3063\u305f\u305f\u3081\u3001\u7d99\u4ee3\u306f\u4e00\u5b9a\u306e\u6761\u4ef6\u3067\u884c\u3046\u3002\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u7d30\u80de\u306e\u751f\u5b58\u7387\u304c\u4e0a\u304c\u308b\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: +RA: Transfection\u3088\u308a6hr\u5f8c\u306b\u901a\u5e38\u57f9\u5730\u306bMC\u3057\u3001\u3055\u3089\u306b30min\u5f8cMC (DMEM + retinoic acid 5 uM)\u3002Transfection\u3088\u308a72hr\u5f8cAssay\u3002
\n\u53c2\u8003\u6587\u732e: J. P. Donahue et al. (1994): The integrin \u03b1v gene: identification and characterization of the promoter region. Biochem. Biophys. Acta 1219, 228-232.
\n\u25a0\u3000LNCaP.FGC<\/span>: \u63a5\u7740\u529b\u304c\u5f31\u304f\u3001\u975e\u5e38\u306b\u306f\u304c\u308c\u3084\u3059\u304b\u3063\u305f\u306e\u3067\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u4f7f\u7528\u3059\u308b\u3002Transfection\u5f8c\u306e\u57f9\u5730\u4ea4\u63db\u306f\u7279\u306b\u5265\u304c\u308c\u3084\u3059\u3044\u3002\u819c\u72b6\u306b\u4e00\u6c17\u306b\u5265\u304c\u308c\u3066\u3057\u307e\u3046\u306e\u3067\u6ce8\u610f\u304c\u5fc5\u8981\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: +dexamethasone: Transfection\u3088\u308a24hr\u5f8cMC (RPMI1640 + dexamethasone 10 uM)\u3002
\n\u53c2\u8003\u6587\u732e: H. Uemura et al. (1995): Identification of a new enhancer in the promoter region of human TR3 orphan receptor gene. J. Biol. Chem. 270, 5427-5433.
\n\u25a0\u3000MIN7<\/span>: \u63a5\u7740\u306b\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u63a5\u7740\u529b\u3082\u5f31\u3044\u306e\u3067transfection\u306e\u969b\u306f\u65e9\u3081\u306b\u7d30\u80de\u3092\u64ad\u7a2e\u3057\u3066\u304a\u304f\u3002
\n\u25a0\u3000MtT\/SM<\/span>: \u534a\u6d6e\u904a\u7d30\u80de\u3067\u3042\u308a\u63a5\u7740\u529b\u304c\u5f31\u3044\u306e\u3067\u3001\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u4f7f\u7528\u3059\u308b\u3002\u7403\u72b6\u306e\u7d30\u80de\u304c\u584a\u306b\u306a\u3063\u3066\u5897\u3048\u3001\u591a\u5c11\u584a\u304c\u5206\u6563\u3057\u306b\u304f\u3044\u304c\u7d30\u80de\u304c\u75db\u307f\u3084\u3059\u3044\u306e\u3067\u7121\u7406\u306b\u307b\u3050\u3055\u306a\u304f\u3066\u3088\u3044\u3002
\n\u25a0\u3000NEC8<\/span>: \u63a5\u7740\u306b\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u63a5\u7740\u529b\u3082\u5f31\u3044\u306e\u3067transfection\u306e\u969b\u306f\u65e9\u3081\u306b\u7d30\u80de\u3092\u64ad\u7a2e\u3057\u3066\u304a\u304f\u3002\u6d6e\u904a\u3057\u305f\u7d30\u80de\u584a\u3082\u751f\u7d30\u80de\u3067\u3042\u308b\u3002
\n\u25a0\u3000PC12<\/span>: \u63a5\u7740\u529b\u306f\u5f31\u3044\u304c\u30b3\u30e9\u30fc\u30b2\u30f3\u30b3\u30fc\u30c8\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u4f7f\u7528\u3059\u308b\u3068\u751f\u7740\u304c\u975e\u5e38\u306b\u3088\u304f\u306a\u3063\u305f\u3002\u30a2\u30c3\u30bb\u30a4\u6642\u3001\u795e\u7d4c\u7cfb\u306b\u5206\u5316\u3055\u305b\u308b\u969b\u306b\u306f\u30dd\u30ea\u30ea\u30b8\u30f3\u30b3\u30fc\u30c8\u30d7\u30ec\u30fc\u30c8\u3092\u4f7f\u7528\u3059\u308b\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: +NGF: Transfection\u3088\u308a6hr\u5f8c\u306b\u901a\u5e38\u57f9\u5730\u306bMC\u3057\u3001\u3055\u3089\u306b30min\u5f8cMC (DMEM + NGF 100 ng\/mL)\u3002
\n\u53c2\u8003\u6587\u732e: M. Mbikay et al. (2002): Characterization of a repressor element in the promoter region of proprotein convertase 2 (PC2) gene. Mol. Brain Res. 102, 35-47.
\n\u25a0\u3000IEC6<\/span>: \u4ed6\u306e\u7d30\u80de\u3068\u540c\u69d8\u306e\u6761\u4ef6\u3067Lipofectamine 2000 (Invitrogen)\u3092\u7528\u3044\u305ftransfection\u3067\u306f\u7d30\u80de\u304c\u6b7b\u6ec5\u3057\u3066\u3044\u307e\u3044\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u6d3b\u6027\u3082\u6e2c\u5b9a\u3067\u304d\u306a\u304b\u3063\u305f\u3002
\n\u25a0\u3000K562<\/span>: \u6d6e\u904a\u7d30\u80de\u3067\u3042\u308atransfection\u5f8c\u306e\u57f9\u5730\u4ea4\u63db\u3067\u7d30\u80de\u3082\u5438\u3063\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308b\u3067\u3001\u57f9\u5730\u6dfb\u52a0\u306b\u5909\u66f4\u3057\u30a2\u30c3\u30bb\u30a4\u6642\u306b\u4e0d\u5fc5\u8981\u5206\u306e\u4e0a\u6e05\u3092\u3068\u308a\u9664\u304f\u307b\u3046\u304c\u3088\u3044\u3002
\n\u5206\u5316\u7528\u57f9\u5730\u306e\u7d44\u6210\u3068\u65b9\u6cd5: +TPA: Transfection\u3088\u308a6hr\u5f8cmedium addition (RPMI1640 + TPA f.c. 50ng\/mL)\u3002
\n\u53c2\u8003\u6587\u732e: M. Villa-Garcia. et al. (1994): Isolation and characterization of a TATA-less promoter for the human \u03b23 integrin gene. Blood 83, 668-676.
\n\u25a0\u3000\u4ee5\u4e0a\u306etransfection\u306f\u3059\u3079\u3066Lipofectamine 2000 (Invitrogen)\u3092\u7528\u3044\u3066\u884c\u3044\u307e\u3057\u305f\u3002\u30ea\u30f3\u9178\u30ab\u30eb\u30b7\u30a6\u30e0\u6cd5\u7b49\u3001\u4ed6\u306e\u65b9\u6cd5\u3067transfection\u3059\u308b\u5834\u5408\u306b\u306f\u57f9\u990a\u6761\u4ef6\u304c\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u4e88\u5099\u5b9f\u9a13\u3092\u884c\u3044\u9069\u5207\u306a\u6761\u4ef6\u3092\u63a2\u3057\u3066\u4e0b\u3055\u3044\u3002
\n\u25a0\u3000\u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3057\u305f\u7d30\u80de\u306e\u3046\u3061MIN7\u306f\u5bae\u5d0e\u7d14\u4e00\u5148\u751f(\u5927\u962a\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7cfb\u7814\u7a76\u79d1)\u3088\u308a\u3054\u4f9b\u4e0e\u3057\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\u3053\u306e\u5834\u3092\u304a\u501f\u308a\u3057\u3066\u304a\u793c\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002\u307e\u305fHela\u3001CHO-K1\u3001 MtT\/SM\u3001NEC8\u3001IEC6\u306f\u7406\u7814 BRC \u7d30\u80de\u6750\u6599\u958b\u767a\u5ba4\u3001MCF7\u306f(\u8ca1)\u30d2\u30e5\u30fc\u30de\u30f3\u30b5\u30a4\u30a8\u30f3\u30b9\u632f\u8208\u8ca1\u56e3 \u7814\u7a76\u8cc7\u6e90\u30d0\u30f3\u30af\u3001HEK293\u3001LNCaP.FGC\u306fATCC\u3088\u308a\u5165\u624b\u3044\u305f\u3057\u307e\u3057\u305f\u3002(M.H. & M.Y.)<\/p>\n