{"id":4496,"date":"2018-10-22T09:45:21","date_gmt":"2018-10-22T00:45:21","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=4496"},"modified":"2018-10-22T09:45:29","modified_gmt":"2018-10-22T00:45:29","slug":"technicalnotes18","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/manual\/technicalnotes18","title":{"rendered":"technicalnotes18"},"content":{"rendered":"

\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3<\/h3>\n

\u25a0\u3000\u7406\u7814\uff24\uff2e\uff21\u30d0\u30f3\u30af\u304c\u63d0\u4f9b\u3059\u308b\u30af\u30ed\u30fc\u30f3\u306f\u3001\u7814\u7a76\u8005\u306e\u7686\u69d8\u304b\u3089\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u3044\u305f\u30af\u30ed\u30fc\u30f3\u306e\u307f\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u5f53\u30d0\u30f3\u30af\u81ea\u3089\u30b2\u30ce\u30e0DNA \u3084cDNA \u3092\u8abf\u88fd\u3057\u3001\u30d9\u30af\u30bf\u30fc\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u3082\u306e\u3082\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u3089\u306f\u300c\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u30d0\u30f3\u30af\u30af\u30ed\u30fc\u30f3\u300d\u3067\u3042\u308a\u300cpCMV_tag and pRSV_tag \u30af\u30ed\u30fc\u30f3\u300d\u3067\u3042\u308a\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u7528\u300c\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u300d\u3067\u3059\u3002\u3053\u308c\u307e\u3067\u306b600\u3092\u8d8a\u3048\u308b\u30af\u30ed\u30fc\u30f3\u3092\u69cb\u7bc9\u3057\u307e\u3057\u305f\u3002
\n\u25a0\u3000\u30af\u30ed\u30fc\u30f3\u3092\u4f5c\u88fd\u3059\u308b\u306b\u306f\u3001\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u4f5c\u696d\u304c\u6b20\u304b\u305b\u307e\u305b\u3093\u3002\u73fe\u5728\u4f7f\u7528\u4e2d\u306e\u9175\u7d20\u306f16\u2103\u3067\u306e\u53cd\u5fdc\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u30b3\u30fc\u30eb\u30c9\u30eb\u30fc\u30e0\u5185\u306b\u7f6e\u3044\u305f16\u2103\u306e\u6052\u6e29\u6c34\u69fd\u3067\u53cd\u5fdc\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u3092\u30d5\u30ed\u30fc\u30c8\u306b\u523a\u3057\u3001\u6c34\u9762\u306b\u6d6e\u304b\u3079\u3066\u53cd\u5fdc\u3092\u3059\u308b\u3068\u3001\u9577\u3044\u53cd\u5fdc\u6642\u9593\u3067\u306f\u84cb\u306b\u6c34\u6ef4\u3092\u4f5c\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u30b3\u30fc\u30eb\u30c9\u30eb\u30fc\u30e0\u5185\u306b\u6052\u6e29\u6c34\u69fd\u3092\u7f6e\u3044\u3066\u3044\u308b\u306e\u3067\u3001\u53cd\u5fdc\u6db2\u306f16\u2103\u306b\u3001\u84cb\u306f4\u2103\u306b\u3055\u3089\u3055\u308c\u3001\u84b8\u767a\u3057\u305f\u53cd\u5fdc\u6db2\u306e\u6c34\u5206\u304c\u3001\u51b7\u5374\u3055\u308c\u305f\u84cb\u306b\u3064\u304f\u304b\u3089\u3067\u3059\u3002\u5168\u304f\u6c34\u304c\u306a\u304f\u306a\u308b\u307e\u3067\u6c34\u5206\u304c\u84b8\u767a\u3059\u308b\u3053\u3068\u306f\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u6b63\u5e38\u306b\u53cd\u5fdc\u3057\u3066\u3044\u308b\u3068\u306f\u304a\u3082\u3048\u307e\u305b\u3093\u3002
\n\u25a0\u3000\u305d\u3053\u3067\u3001\u30c1\u30e5\u30fc\u30d6\u3092\u6c34\u306e\u4e2d\u306b\u6c88\u3081\u3066\u3084\u308c\u3070\u3053\u306e\u554f\u984c\u306f\u89e3\u6c7a\u3057\u307e\u3059\u3002\u307e\u305a\u300150 mL \u306e\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u30c1\u30e5\u30fc\u30d6\u306b\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u3092\u8a70\u3081\u3066\u6c88\u3081\u308b\u3053\u3068\u3092\u8003\u3048\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u6d6e\u529b\u306e\u3042\u308b50 mL \u30c1\u30e5\u30fc\u30d6\u3092\u6c88\u3081\u308b\u306e\u306f\u3072\u3068\u82e6\u52b4\u3067\u3059\u3002\u91cd\u3057\u3092\u7f6e\u3053\u3046\u306b\u3082\u3001\u4e38\u3044\u30c1\u30e5\u30fc\u30d6\u306b\u5b89\u5b9a\u3057\u3066\u4e57\u3063\u3066\u304f\u308c\u308b\u91cd\u3057\u3092\u898b\u3064\u3051\u308b\u3053\u3068\u306f\u51fa\u6765\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u6c88\u3081\u308b\u3082\u306e\u306b\u7a7a\u6c17\u304c\u3042\u308b\u304b\u3089\u6d6e\u529b\u304c\u3042\u308b\u3068\u6c17\u3065\u304d\u3001\u306a\u308b\u3079\u304f\u7a7a\u6c17\u3092\u629c\u3044\u305f\u5bb9\u5668\u306b\u3059\u308b\u305f\u3081\u306b\u3001\u6b21\u306b\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u3092\u30cf\u30a4\u30d6\u30ea\u30d0\u30c3\u30b0\u306b\u5165\u308c\u3066\u30b7\u30fc\u30eb\u3057\u307e\u3057\u305f\u3002\u7a7a\u6c17\u3092\u629c\u3044\u3066\u6c88\u307f\u3084\u3059\u304f\u3057\u305f\u3082\u306e\u306f\u3067\u304d\u305f\u306e\u3067\u3059\u304c\u3001\u6bce\u56de\u3001\u71b1\u30b7\u30fc\u30eb\u3092\u3059\u308b\u306e\u306f\u9762\u5012\u3067\u3059\u3057\u3001\u6d6e\u529b\u3092\u6291\u3048\u305f\u3068\u306f\u3044\u3048\u3001\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u81ea\u8eab\u306e\u7a7a\u6c17\u5206\u3092\u629c\u304f\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\u6c88\u3081\u308b\u305f\u3081\u306e\u91cd\u3057\u3092\u63a2\u3055\u306a\u3051\u308c\u3070\u3044\u3051\u306a\u3044\u3053\u3068\u306b\u306f\u5909\u308f\u308a\u3042\u308a\u307e\u305b\u3093\u3002
\n\u25a0\u3000\u4f55\u304b\u305d\u308c\u81ea\u8eab\u304c\u91cd\u3044\u5bb9\u5668\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3001\u624b\u3054\u308d\u306a\u30ac\u30e9\u30b9\u88fd\u54c1\u3092\u63a2\u3057\u3066\u3044\u305f\u3068\u3053\u308d\u3001\u30b8\u30e3\u30e0\u306e\u30d3\u30f3\u3092\u601d\u3044\u3064\u304d\u307e\u3057\u305f\u3002\u30b8\u30e3\u30e0\u306e\u30d3\u30f3\u306f\u81ea\u91cd\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u84cb\u3092\u3057\u305f\u3068\u304d\u306b\u305d\u308c\u81ea\u8eab\u3067\u6c88\u3080\u307b\u3069\u306f\u91cd\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u5c11\u3057\u5927\u304d\u3081\u306e\u30ca\u30c3\u30c8\u3084\u91e3\u308a\u7528\u306e\u30aa\u30e2\u30ea\u3092\u5165\u308c\u308b\u3068\u3001\u6c34\u69fd\u306e\u5e95\u306b\u6c88\u307f\u3001\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u3092\u7acb\u3066\u308b\u305f\u3081\u306e\u7a7a\u9593\u3082\u3042\u308a\u307e\u3059\u3002\u5e83\u53e3\u3067\u8089\u539a\u306e150 mL \u7a0b\u306e\u3082\u306e\u304c\u304a\u52e7\u3081\u3067\u3059 (T.M.)
\n(Mail News 2008.05.02 \u63b2\u8f09\u8a18\u4e8b\u3088\u308a)<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3 \u25a0\u3000\u7406\u7814\uff24\uff2e\uff21\u30d0\u30f3\u30af\u304c\u63d0\u4f9b\u3059\u308b\u30af\u30ed\u30fc\u30f3\u306f\u3001\u7814\u7a76\u8005\u306e\u7686\u69d8\u304b\u3089\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u3044\u305f\u30af\u30ed\u30fc\u30f3\u306e\u307f\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u5f53\u30d0\u30f3\u30af\u81ea\u3089\u30b2\u30ce\u30e0DNA \u3084cDNA \u3092\u8abf\u88fd\u3057\u3001\u30d9\u30af\u30bf\u30fc\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u3082\u306e\u3082\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u3089\u306f\u300c\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u30d0\u30f3\u30af\u30af\u30ed\u30fc\u30f3\u300d\u3067\u3042\u308a\u300cpCMV_tag and pRSV_tag \u30af\u30ed\u30fc\u30f3\u300d\u3067\u3042\u308a\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u7523\u751f\u7528\u300c\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u300d\u3067\u3059\u3002\u3053\u308c\u307e\u3067\u306b600\u3092\u8d8a\u3048\u308b\u30af […]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":296,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-4496","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4496","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=4496"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4496\/revisions"}],"predecessor-version":[{"id":4498,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4496\/revisions\/4498"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/296"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=4496"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}