{"id":4488,"date":"2018-10-22T09:42:27","date_gmt":"2018-10-22T00:42:27","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/ja\/?page_id=4488"},"modified":"2023-06-13T09:26:44","modified_gmt":"2023-06-13T00:26:44","slug":"technicalnotes11","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/manual\/technicalnotes11","title":{"rendered":"\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8a71\u3057"},"content":{"rendered":"<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8a71\u3057 (1)<\/h3>\n<p>\u25a0\u3000RIKEN DNA Bank\u306e\u7279\u5fb4\u306e\u4e00\u3064\u306f\u3001\u8c4a\u5bcc\u306a\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8cc7\u6750\u3001\u5373\u3061\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306a\u3089\u3073\u306b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u3067\u3059\u3002\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306a\u3089\u3073\u306bDNA Bank web catalog \u3092\u5229\u7528\u3057\u3066\u3001\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u306e\u69cb\u7bc9\u306e\u305f\u3081\u306b\u7528\u3044\u305f\u30af\u30ed\u30fc\u30f3\u3092\u305f\u3069\u308b\u3053\u3068\u3082\u51fa\u6765\u307e\u3059\u3002\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u8ca9\u58f2\u3059\u308b\u4f1a\u793e\u3082\u5c11\u3057\u305a\u3064\u5897\u3048\u3066\u304d\u307e\u3057\u305f\u304c\u3001RIKEN DNA Bank\u306e\u3088\u3046\u306b \u69d8\u3005\u306a\u907a\u4f1d\u5b50\u306e\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3068\u305d\u306e\u95a2\u9023\u6750\u6599\u3092\u6271\u3063\u3066\u3044\u308b\u30d0\u30f3\u30af\u306f\u4ed6\u306b\u3042\u308a\u307e\u305b\u3093\u3002 (<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0046j#vector_adeno\">\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u306e\u53c2\u8003\u8a18\u4e8b<\/a>)<br \/>\n\u25a0\u3000RIKEN DNA Bank\u3067\u306f\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u6cd5\u3068\u3057\u3066\u3001COS-TPC\u6cd5\u3068\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\u3092\u63a1\u7528\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n\u25a0\u3000\u65e7\u6765\u6cd5\u306eCOS-TPC \u6cd5\u306f\u3001\uff12\u7a2e\u985e\u306eDNA \u3092\u5bbf\u4e3b\u7d30\u80de\u3067\u3042\u308bHEK293 \u7d30\u80de\u306b\u5c0e\u5165\u3057\u3066\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u5f97\u307e\u3059\u3002\u3072\u3068\u3064\u306f\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u306e\u307b\u307c\u5168\u9577\u3092\u6301\u3063\u305f\u30b3\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3067\u3001E1 \u9818\u57df\u3092\u6b20\u5931\u3057\u3066\u3044\u307e\u3059\u3002\u3082\u3046\u3072\u3068\u3064\u306fEcoT22I (+ClaI) \u306b\u3088\u308a\u5236\u9650\u9175\u7d20\u6d88\u5316\u3057\u305f\u672b\u7aef\u30bf\u30f3\u30d1\u30af\u4ed8\u304dAd5-dlx \u30b2\u30ce\u30e0 (DNA\u2010TPC)\u3067\u3001E1\u9818\u57df\u306f\u5236\u9650\u9175\u7d20\u51e6\u7406\u3067\u5207\u65ad\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\u3053\u306e\uff12\u7a2e\u985e\u306eDNA \u304cHEK293\u7d30\u80de\u306b\u5c0e\u5165\u3055\u308c\u308b\u3068\u3001\u7d30\u80de\u5185\u76f8\u540c\u7d44\u63db\u3048\u306b\u3088\u308a\u76ee\u7684\u306e\u767a\u73fe\u30e6\u30cb\u30c3\u30c8\u3092\u642d\u8f09\u3057\u305f\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u304c\u7523\u751f\u3055\u308c\u307e\u3059\u3002\u3053\u3053\u3067\u306e\u554f\u984c\u70b9\u306f\u3001DNA-TPC \u306e\u5236\u9650\u9175\u7d20\u51e6\u7406\u304c\u4e0d\u5341\u5206\u3060\u3068\u3001DNA-TPC \u7531\u6765\u306e\u30a6\u30a4\u30eb\u30b9\u521d\u671f\u907a\u4f1d\u5b50E1A \u3068E1B \u3092\u6301\u3063\u305f\u81ea\u5f8b\u5897\u6b96\u53ef\u80fd\u306a\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9 (RCA; Replication Competent Adenovirus) \u304c\u751f\u6210\u3057\u3066\u304d\u3066\u3057\u307e\u3046\u3053\u3068\u3067\u3059\u3002RCA \u306e\u5206\u96e2\u306b\u306f\u975e\u5e38\u306b\u624b\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u3057\u3001\u304b\u308a\u306b\u691c\u51fa\u9650\u754c\u4ee5\u4e0b\u3067\u3082\u6df7\u5165\u3057\u3066\u3044\u305f\u3068\u3057\u305f\u30892\u306a\u3044\u30573\u4e16\u4ee3\u5f8c\u306b\u306f\u307b\u3068\u3093\u3069\u304cRCA positive \u3068\u306a\u3063\u3066\u3057\u307e\u3044\u3001\u76ee\u7684\u306e\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u5206\u96e2\u3059\u308b\u3053\u3068\u304c\u51fa\u6765\u306a\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002<br \/>\n\u25a0\u3000\u305d\u3053\u3067\u3001\u4f5c\u696d\u306e\u52b9\u7387\u5316\u3001\u7c21\u4fbf\u5316\u3092\u76ee\u7684\u3068\u3057\u3066\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u306bDNA\u2010TPC\u3092\u7528\u3044\u308b\u5fc5\u8981\u306e\u306a\u3044\u5b8c\u5168\u9577\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u3092\u6301\u3063\u305fit\u30d9\u30af\u30bf\u30fc (intact termini vector)\u304c\u958b\u767a\u3055\u308c\u307e\u3057\u305f\u3002\u3053\u306eit \u30d9\u30af\u30bf\u30fc\u306b\u306f\u30d2\u30c8\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b95\u578b\u30b2\u30ce\u30e0\u304c\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001E1 \u9818\u57df\u306f\u6b20\u640d\u3057\u3066\u3044\u307e\u3059\u3002\u5f93\u3063\u3066\u3001it (intact termini)\u30d9\u30af\u30bf\u30fc\uff08pAxcwit\u3001pAxCAwtit\uff09\u3092\u7528\u3044\u305f\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u3067\u306fRCA\u304c\u51fa\u73fe\u3057\u306b\u304f\u3044\u3068\u3044\u3046\u3053\u3068\u306f\u7406\u8ad6\u7684\u306b\u8003\u3048\u3066\u5f53\u7136\u306e\u3053\u3068\u3067\u3059\u3002\u4eee\u306bRCA \u304c\u751f\u6210\u3059\u308b\u3068\u3059\u308c\u3070\u3001\u30a6\u30a4\u30eb\u30b9\u5897\u6b96\u5bbf\u4e3b\u306eHEK293\u7d30\u80de\u304c\u30b2\u30ce\u30e0\u306b\u6301\u3064E1\u907a\u4f1d\u5b50\u3092\u30a6\u30a4\u30eb\u30b9\u304c\u5897\u6b96\u904e\u7a0b\u3067\u7d44\u63db\u3048\u306b\u3088\u308a\u7372\u5f97\u3059\u308b\u5834\u5408\u3067\u3059\u3002\u3053\u306e\u73fe\u8c61\u306f\u6975\u3081\u3066\u4f4e\u3044\u78ba\u7387\u3067\u3057\u304b\u8d77\u3053\u3089\u305a\u3001\u7d30\u80de\u306e\u7d99\u4ee3\u56de\u6570\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u7d99\u4ee3\u56de\u6570\u306b\u6c17\u3092\u3064\u3051\u3066\u3044\u308c\u3070\u307b\u3068\u3093\u3069\u8d77\u3053\u3089\u306a\u3044\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u901a\u5e38\u3001\u7686\u69d8\u304c\u5b9f\u9a13\u306b\u304a\u4f7f\u3044\u306b\u306a\u308b\u30a6\u30a4\u30eb\u30b9\u306e\u7d99\u4ee3\u56de\u6570\u3067\u306f\u5168\u304f\u554f\u984c\u304c\u306a\u3044\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u3067\u306f\u3001\u672c\u5f53\u306b\u305d\u3046\u306a\u306e\u304b\uff1f\u30c7\u30fc\u30bf\u3068\u3057\u3066\u767a\u8868\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u306e\u3067\u4eca\u56de\u6539\u3081\u3066\u691c\u8a3c\u5b9f\u9a13\u3092\u884c\u3044\u307e\u3057\u305f\u3002<br \/>\n\u25a0\u3000\u7570\u306a\u308b\u30b3\u30b9\u30df\u30c9\u682a\u304b\u3089\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\u306b\u3088\u3063\u3066\u4f5c\u88fd\u3057\u305fGFP \u767a\u73fe\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9 (AxCAEGFPit) 3\u682a\u306b\u3064\u3044\u3066\u30013rd seed\u304b\u308913th seed\u307e\u3067\u30926cm\u30c7\u30a3\u30c3\u30b7\u30e5\u306eHEK293\u7d30\u80de\u306b\u3066\u7d99\u4ee3\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u30a6\u30a4\u30eb\u30b9\u306bRCA \u304c\u767a\u751f\u3057\u3066\u3044\u308b\u304b\u3092\u691c\u67fb\u3057\u307e\u3057\u305f\u3002\u4f5c\u88fd\u3057\u305f\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u3092HeLa \u7d30\u80de\u306b\u611f\u67d3\u3057\u3066\u5f97\u305fcell pack \u3088\u308a\u30a6\u30a4\u30eb\u30b9DNA \u3092\u62bd\u51fa\u3057\u307e\u3057\u305f\u3002E1A \u306a\u3089\u3073\u306bE1B \u3092\u691c\u51fa\u3067\u304d\u308b\u3088\u3046\u8a2d\u8a08\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3092\u7528\u3044\u3066PCR \u306b\u3088\u308aRCA \u306e\u691c\u51fa\u3092\u8a66\u307f\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u30013\u682a\u3068\u3082\u306b13\u4ee3\u307e\u3067\u306fRCA \u306f\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u307e\u305f\u3001\u7d30\u80de\u89b3\u5bdf\u3067\u308213\u4ee3\u307e\u3067EGFP \u306e\u767a\u73fe\u306f\u9ad8\u3044\u30ec\u30d9\u30eb\u306b\u4fdd\u305f\u308c\u3066\u304a\u308a\u3001\u5b89\u5b9a\u3057\u3066\u3044\u307e\u3057\u305f\u3002(\u691c\u67fb\u624b\u6cd5\u306e\u53c2\u8003\u8a18\u4e8b: \u300c<a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Adv\/Adv-004ja.html\">\u633f\u5165\u907a\u4f1d\u5b50\u3068RCA\u306e\u78ba\u8a8d [link]<\/a>\u300d\u306a\u3089\u3073\u306b\u300c<a href=\"https:\/\/dnaconda.riken.jp\/rvd\/SOP\/Adv\/adenovirus3.pdf\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u30de\u30cb\u30e5\u30a2\u30eb \uff5e\u30af\u30ed\u30fc\u30f3\u5316\u30b2\u30ce\u30e0\u5c0e\u5165\u6cd5\uff5e [PDF]<\/a>\u300d)<br \/>\n\u25a0\u3000 \u4eca\u56de\u4f7f\u7528\u3057\u305f\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528it \u30d9\u30af\u30bf\u30fc\u306f\u30b3\u30b9\u30df\u30c9\u30d9\u30fc\u30b9\u306e\u30af\u30ed\u30fc\u30f3\u3067\u3042\u308a\u3001\u53d6\u6271\u3044\u306b\u30b3\u30c4\u304c\u5fc5\u8981\u3067\u3059\u3002\u3042\u307e\u308a\u9577\u3044\u6642\u9593\u5927\u8178\u83cc\u3092\u57f9\u990a\u3059\u308b\u3068\u767a\u73fe\u30e6\u30cb\u30c3\u30c8\u304c\u843d\u3061\u3066\u3057\u307e\u3063\u305f\u308a\u3001\u64cd\u4f5c\u4e2d\u3084\u4fdd\u5b58\u4e2d\u306e\u72b6\u614b\u306b\u3088\u3063\u3066\u306fDNA \u304c\u5207\u308c\u3066\u3057\u307e\u3063\u305f\u308a\u3059\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u4e00\u5ea6\u30a6\u30a4\u30eb\u30b9\u306b\u306a\u308c\u3070DNA \u306b\u5909\u7570\u304c\u5165\u3063\u305f\u3082\u306e\u306f\u5897\u3048\u305a\u3001RCA\u3082\u51fa\u73fe\u78ba\u7387\u306f\u975e\u5e38\u306b\u4f4e\u304f\u3001\u76ee\u7684\u30a6\u30a4\u30eb\u30b9\u306e\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u6b96\u304c\u4fdd\u305f\u308c\u3084\u3059\u3044\u305f\u3081\u3001\u6271\u3044\u3084\u3059\u3044\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u601d\u3044\u307e\u3059\u3002<br \/>\n\u25a0\u3000 \u5f53\u30d0\u30f3\u30af\u3067\u306fit\u30d9\u30af\u30bf\u30fc\u3084\u305d\u306e\u6539\u826f\u7248\u3067\u3042\u308bit2\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u3066\u91cd\u8981cDNA\u642d\u8f09\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u4f5c\u88fd\u3057\u3001\u63d0\u4f9b\u3057\u3066\u3044\u304f\u4e88\u5b9a\u3067\u3059\u3002 (M.T. &#038; T.M.)<br \/>\n(Mail News 2006.06.30 \u63b2\u8f09\u8a18\u4e8b\u3088\u308a)<\/p>\n<h3>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8a71\u3057 (2)<\/h3>\n<p>\u25a0\u3000\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u4f7f\u3048\u3070\u3001\u9ad8\u3044\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u304c\u671f\u5f85\u3067\u304d\u308b\u3002\u5b66\u4f1a\u3084\u30bb\u30df\u30ca\u30fc\u306a\u3069\u3067\u30e6\u30fc\u30b6\u30fc\u306e\u7686\u3055\u3093\u3068\u304a\u8a71\u3057\u3059\u308b\u3068\u3001\u305d\u3046\u671f\u5f85\u3055\u308c\u3066\u3044\u308b\u304a\u8a71\u3092\u591a\u304f\u805e\u304d\u307e\u3059\u3002\u78ba\u304b\u306b\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u306e\u60aa\u3044\u30de\u30a6\u30b9\u521d\u4ee3\u57f9\u990a\u7d30\u80de (MEF) \u306e\u5834\u5408\u306f\u3001\u6211\u3005\u304c\u884c\u3063\u305f\u5b9f\u9a13\u3067\u7d75\u306f\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u304c\u4e0a\u304c\u308a\u307e\u3057\u305f\u3002<br \/>\n\u25a0\u3000\u3057\u304b\u3057\u3001\u9ad8\u3044\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u304c\u671f\u5f85\u3067\u304d\u308b\u306e\u306f\u3001\u5168\u3066\u306e\u7d30\u80de\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u7d30\u80de\u306b\u3088\u3063\u3066\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u306f\u307e\u3061\u307e\u3061\u3067\u3059\u3002\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u4f7f\u308f\u308c\u3066\u3044\u308b\u30d2\u30c8\uff15\u578b\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u3001\u30b3\u30af\u30b5\u30c3\u30ad\u30fc\u30a6\u30a4\u30eb\u30b9\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u53d7\u5bb9\u4f53 (CAR) \u3092\u4ecb\u3057\u3066\u7d30\u80de\u306b\u5c0e\u5165\u3055\u308c\u307e\u3059\u3002\u305d\u3053\u3067\u3001CAR \u306e\u767a\u73fe\u304c\u5c11\u306a\u3044\u7d30\u80de\u3067\u306f\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306f\u53d6\u308a\u8fbc\u307e\u308c\u306b\u304f\u304f\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u8840\u7403\u7cfb\u7d30\u80de\u306b\u306f\u5165\u308a\u306b\u304f\u3044\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0046j#reference_adeno\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u53c2\u8003\u8cc7\u6599<\/a><br \/>\n\u25a0\u3000\u6700\u8fd1\u306eCancer Gene Therapy \u306e\u76ee\u6b21\u3092\u898b\u3066\u3082\u3001\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u7279\u5b9a\u306e\u7d30\u80de\u3078\u306e\u611f\u67d3\u52b9\u7387\u3092\u4e0a\u3052\u308b\u52aa\u529b\u304c\u7d9a\u3044\u3066\u3044\u307e\u3059\u3002\u8a00\u3044\u63db\u3048\u308c\u3070\u3001\u3053\u308c\u3089\u306e\u8ad6\u6587\u306b\u51fa\u3066\u304f\u308b\u7d30\u80de\u3092\u4f7f\u3046\u4e88\u5b9a\u306e\u65b9\u306f\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u304c\u3042\u307e\u308a\u9ad8\u304f\u306a\u3044\u3053\u3068\u3092\u899a\u609f\u3057\u306a\u3051\u308c\u3070\u3044\u3051\u307e\u305b\u3093\u3002<\/p>\n<ul>\n<li>Z. Ye et al., Engineered CD8+ cytotoxic T cells with fiber-modified adenovirus-mediated TNF- gene transfection counteract immunosuppressive interleukin-10-secreting lung metastasis and solid tumors. Cancer Gene Ther 14: 661-675, 2007.<\/li>\n<li>K. Rittner et al., Targeting of adenovirus vectors carrying a tumor cell-specific peptide: in vitro and in vivo studies. Cancer Gene Ther 14: 509-518, 2007.<\/li>\n<li>M. K. Magnusson et al., Adenovirus 5 vector genetically re-targeted by an Affibody molecule with specificity for tumor antigen HER2\/neu. Cancer Gene Ther 14: 468-479, 2007.<\/li>\n<li>M. Stevenson et al., Incorporation of a laminin-derived peptide (SIKVAV) on polymer-modified adenovirus permits tumor-specific targeting via 6-integrins. Cancer Gene Ther 14: 335-345, 2007.<\/li>\n<li>E. Brouwer et al., Human adenovirus type 35 vector for gene therapy of brain cancer: improved transduction and bypass of pre-existing anti-vector immunity in cancer patients. Cancer Gene Ther 14: 211-219, 2007.<\/li>\n<li>S. Ni et al., Evaluation of adenovirus vectors containing serotype 35 fibers for tumor targeting. Cancer Gene Ther 13: 1072-1081, 2006.<\/li>\n<li>W. A. Marsman et al., Gene therapy for barrett\u2019s esophagus: adenoviral gene transfer in different intestinal models. Cancer Gene Ther 12: 778-786, 2005.<\/li>\n<li>Y. Okada et al., Transcriptional targeting of RGD fiber-mutant adenovirus vectors can improve the safety of suicide gene therapy for murine melanoma. Cancer Gene Ther 12: 608-616, 2005.<\/li>\n<li>A. V. Borovjagin et al., Complex mosaicism is a novel approach to infectivity enhancement of adenovirus type 5-based vectors. Cancer Gene Ther 12: 475-486, 2005.<\/li>\n<li>B. O. Engesater et al., PCI-enhanced adenoviral transduction employs the known uptake mechanism of adenoviral particles. Cancer Gene Ther 12: 439-448, 2005.<\/li>\n<\/ul>\n<p>\u25a0\u3000\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3067\u306f\u3001\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u95a2\u3059\u308b\u7279\u6027\u60c5\u5831\u306e\u53ce\u96c6\u306b\u52aa\u3081\u3001\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u52b9\u679c\u7684\u306b\u4f7f\u3046\u305f\u3081\u306e\u60c5\u5831\u3092\u84c4\u7a4d\u3057\u3066\u3044\u307e\u3059\u3002\u8a08\u753b\u3055\u308c\u3066\u3044\u308b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u5b9f\u9a13\u304c\u76ee\u7684\u306e\u7d30\u80de\u306b\u5408\u3063\u305f\u624b\u6cd5\u304b\u3069\u3046\u304b\u3001\u8ff7\u3063\u305f\u3068\u304d\u306b\u306f\uff24\uff2e\uff21\u30d0\u30f3\u30af\u307e\u3067\u96fb\u5b50\u30e1\u30fc\u30eb\u3067\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002(T.M.)<br \/>\n(Mail News 2007.11.01 \u63b2\u8f09\u8a18\u4e8b\u3088\u308a)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8a71\u3057 (1) \u25a0\u3000RIKEN DNA Bank\u306e\u7279\u5fb4\u306e\u4e00\u3064\u306f\u3001\u8c4a\u5bcc\u306a\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8cc7\u6750\u3001\u5373\u3061\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306a\u3089\u3073\u306b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u4f5c\u88fd\u7528\u30b7\u30e3\u30c8\u30eb\u30d9\u30af\u30bf\u30fc\u3067\u3059\u3002\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306a\u3089\u3073\u306bDNA Bank web catalog \u3092\u5229\u7528\u3057\u3066\u3001\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u306e\u69cb\u7bc9\u306e\u305f\u3081\u306b\u7528\u3044\u305f\u30af\u30ed\u30fc\u30f3\u3092\u305f\u3069\u308b\u3053\u3068\u3082\u51fa\u6765\u307e\u3059\u3002\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3092\u8ca9\u58f2\u3059\u308b\u4f1a\u793e\u3082\u5c11\u3057\u305a\u3064\u5897\u3048 [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":296,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-4488","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4488","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=4488"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4488\/revisions"}],"predecessor-version":[{"id":5638,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4488\/revisions\/5638"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/296"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=4488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}