{"id":296,"date":"2013-02-27T16:55:19","date_gmt":"2013-02-27T07:55:19","guid":{"rendered":"http:\/\/dna.brc.riken.jp:8001\/?page_id=296"},"modified":"2021-04-21T10:31:01","modified_gmt":"2021-04-21T01:31:01","slug":"manual","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/manual","title":{"rendered":"\u30ea\u30bd\u30fc\u30b9\u306e\u53d6\u308a\u6271\u3044"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<ul>\n<li><a href=\"#daichou\">\u5927\u8178\u83cc\u306e\u53d6\u6271\u3044<\/a><\/li>\n<li><a href=\"#plasmid\">\u30d7\u30e9\u30b9\u30df\u30c9\u3001\u30b3\u30b9\u30df\u30c9\u3001BAC\u30af\u30ed\u30fc\u30f3<\/a><\/li>\n<li><a href=\"#genomic\">\u30b2\u30ce\u30e0DNA<\/a><\/li>\n<li><a href=\"#honyu\">\u54fa\u4e73\u52d5\u7269\u57f9\u990a\u7d30\u80de<\/a><\/li>\n<li><a href=\"#adeno\">\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9<\/a><\/li>\n<li><a href=\"#tech_h\">Technical notes<\/a><\/li>\n<li><a href=\"#ronbun\">\u5bc4\u8a17\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u3092\u7528\u3044\u305f\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb<\/a><\/li>\n<li><a href=\"#reference\">Reference of Genetic Materials<\/a><\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"daichou\"><\/a><\/p>\n<h3>\u5927\u8178\u83cc\u306e\u53d6\u6271\u3044<\/h3>\n<ul>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0004j\">\u5927\u8178\u83cc\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0016j\">\u5927\u8178\u83cc\u7d44\u63db\u3048\u86cb\u767d\u8cea\u306e\u8abf\u88fd<\/a><\/li>\n<li>\u30b9\u30bf\u30d6\u5bd2\u5929\u304b\u3089\u306e\u83cc\u306e\u57f9\u990a<br \/>\n\u767d\u91d1\u8033\u3067\u30b9\u30bf\u30d6\u5bd2\u5929\u306e\u83cc\u306e\u751f\u3048\u3066\u3044\u308b\u3068\u3053\u308d\u306b\u7a81\u304d\u523a\u3057\u3066\u83cc\u3092\u3068\u308a\u3001\u5bd2\u5929\u30d7\u30ec\u30fc\u30c8\u306b\u30b9\u30c8\u30ea\u30fc\u30af\u3057\u3001\u57f9\u990a\u3057\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/strain.gif\" width=\"200\" height=\"311\" \/>\n<\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"plasmid\"><\/a><a name=\"cosmid\"><\/a><a name=\"bac\"><\/a><\/p>\n<h3>\u30d7\u30e9\u30b9\u30df\u30c9\u3001\u30b3\u30b9\u30df\u30c9\u3001BAC \u30af\u30ed\u30fc\u30f3<\/h3>\n<ul>\n<li><a title=\"\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u53d6\u6271\u3044\" href=\"https:\/\/brc.riken.jp\/dna\/MAN0005j\">\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u53d6\u6271\u3044<\/a><\/li>\n<li><a title=\"\u30b3\u30b9\u30df\u30c9\u306e\u53d6\u6271\u3044\" href=\"https:\/\/brc.riken.jp\/dna\/MAN0006j\">\u30b3\u30b9\u30df\u30c9\u306e\u53d6\u6271\u3044<\/a><\/li>\n<li><a title=\"BAC DNA\u8abf\u88fd\" href=\"https:\/\/brc.riken.jp\/dna\/MAN0001j\">BAC \u30af\u30ed\u30fc\u30f3\u306e\u53d6\u308a\u6271\u3044<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"genomic\"><\/a><\/p>\n<h3>\u30b2\u30ce\u30e0DNA<\/h3>\n<ul>\n<li><a title=\"\u5fae\u751f\u7269\u30b2\u30ce\u30e0DNA\u306e\u8abf\u88fd\" href=\"https:\/\/brc.riken.jp\/dna\/MAN0007j\">\u5fae\u751f\u7269\u30b2\u30ce\u30e0DNA\u306e\u8abf\u88fd<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"honyu\"><\/a><\/p>\n<h4>\u54fa\u4e73\u52d5\u7269\u57f9\u990a\u7d30\u80de<\/h4>\n<ul>\n<li><a title=\"\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u4f7f\u7528\" href=\"https:\/\/brc.riken.jp\/dna\/MAN0008j\">\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u4f7f\u7528, \u57f9\u990a\u7d30\u80de\u306e\u30e9\u30a4\u30bb\u30fc\u30c8\u306e\u8abf\u88fd<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0009j\">Luciferase assay\u306e\u30b3\u30c4<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"adeno\"><\/a><\/p>\n<h3>\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9<\/h3>\n<ul>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0011j\">\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306e\u8a71\u3057<\/a><\/li>\n<li><a title=\"\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u6a19\u6e96\u64cd\u4f5c\u624b\u9806\u66f8\u96c6\" href=\"https:\/\/brc.riken.jp\/dna\/MAN0002j\">\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u96c6\uff08\u65e5\u672c\u8a9e\uff09<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0002e\" target=\"_blank\" rel=\"noopener noreferrer\">\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u96c6\uff08\u82f1\u8a9e\uff09<\/a><\/li>\n<li><a title=\"\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\" href=\"https:\/\/brc.riken.jp\/dna\/GRP0046j\">\u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9\u306e\u53d6\u6271\u3044<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0052j\">\u4e09\u597d\u6d69\u4e4b\u535a\u58eb\u304c\u958b\u767a\u3057\u305f\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc [link]<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"tech_h\"><\/a><a name=\"tech_p\"><\/a><\/p>\n<h3>Technical notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0012j\">PCR\u306e\u8a71\u3057<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0003j\">\u5869\u57fa\u914d\u5217\u89e3\u6790<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0017j\">ponceau S<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0018j\">\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0015j\" target=\"_blank\" rel=\"noopener noreferrer\">\u30af\u30ed\u30de\u30c1\u30f3\u514d\u75ab\u6c88\u964d\u6cd5 (ChIP)-PCR [pdf]<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<h3>Sequencing &amp; PCR primer<\/h3>\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0035e\">Sequencing &amp; PCR primers<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0007j\">Primers for PCR of rRNA gene (Microbe Genomic DNA Information)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/rvd\/stsmkr.html\">List of STS Markers for Detection of Virus Genes<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#head\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"ronbun\"><\/a><\/p>\n<h2>\u5bc4\u8a17\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u3092\u7528\u3044\u305f\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb<\/h2>\n<ul style=\"line-height: 1.5em;\">\n<li><span style=\"text-decoration:underline;\">\u7d44\u63db\u3048\u30ac\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3068\u7cbe\u88fd<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GSB0048e\">Lectin (GSB0048) &#8211; Gene Set Collection<\/a><\/td>\n<td>\u897f \u671b\u300c\u7d44\u63db\u3048\u30ac\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3068\u7cbe\u88fd\u306b\u95a2\u3059\u308b\u30ce\u30fc\u30c8\u300d Glycoforum Vol.23 (5), A15 (2020). DOI: https:\/\/doi.org\/10.32285\/glycoforum.23A15J.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\"><a href=\"http:\/\/dna.brc.riken.jp\/DataSheet\/MAN0021j\">\u30a2\u30ab\u30cd\u30ba\u30df\uff08Apodemus speciosus\uff09\u67d3\u8272\u4f53\u7279\u7570\u7684 DNA \u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u305f FISH \u306b\u3088\u308b\u67d3\u8272\u4f53\u30da\u30a4\u30f3\u30c6\u30a3\u30f3\u30b0\u6cd5 (\u4e45\u4fdd\u7530 \u5584\u4e45\u535a\u58eb) [pdf]<\/a><\/span><br \/>\nKawagoshi, T., Chromosomal Aberrations in Large Japanese Field Mice (Apodemus speciosus) Captured near Fukushima Dai-ichi Nuclear Power Plant. Environ. Sci. Technol. 51 (8): 4632-4641 (2017). PMID 28363014.<\/p>\n<table>\n<tbody>\n<tr>\n<td Align=\"Left\">Catalog #<\/td>\n<td Align=\"Left\">Name of clone<\/td>\n<td Align=\"Left\">Running title<\/td>\n<\/tr>\n<tr bgcolor=\"#ffffff\">\n<td>RDB16699<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16699\">Painting probe DNA for A. speciosus chromosome 1<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 1.<\/td>\n<\/tr>\n<tr>\n<td>RDB16700<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16700\">Painting probe DNA for A. speciosus chromosome 2<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 2.<\/td>\n<\/tr>\n<tr bgcolor=\"#ffffff\">\n<td>RDB16701<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16701\">Painting probe DNA for A. speciosus chromosome 3 and 4<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 3 and 4.<\/td>\n<\/tr>\n<tr>\n<td>RDB16702<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16702\">Painting probe DNA for A. speciosus chromosome 5<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 5.<\/td>\n<\/tr>\n<tr bgcolor=\"#ffffff\">\n<td>RDB16703<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16703\">Painting probe DNA for A. speciosus chromosome 6<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 6.<\/td>\n<\/tr>\n<tr>\n<td>RDB16704<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16704\">Painting probe DNA for A. speciosus chromosome 7<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 7.<\/td>\n<\/tr>\n<tr bgcolor=\"#ffffff\">\n<td>RDB16705<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16705\">Painting probe DNA for A. speciosus chromosome 8<\/a><\/td>\n<td>Painting probe DNA for A. speciosus chromosome 8.<\/td>\n<\/tr>\n<tr>\n<td>RDB16706<\/td>\n<td><a Href=\"https:\/\/brc.riken.jp\/dna\/RDB16706\">Painting probe DNA for A. speciosus X chromosome<\/a><\/td>\n<td>Painting probe DNA for A. speciosus X chromosome.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\"><a href=\"https:\/\/brc.riken.jp\/dna\/MAN0020e\">Purification of Recombinant Tubulin by Dr. Etsuko Muto [pdf]<\/a><\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<tr>\n<td>RDB12753<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12753\">pFBD-Hs alpha1CGH\/beta3CGF<\/a><\/td>\n<td>Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin.<\/td>\n<\/tr>\n<tr>\n<td>RDB12754<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12754\">pFBD-Hs alpha1(K40R)CGH\/beta3CGF<\/a><\/td>\n<td>Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin.<\/td>\n<\/tr>\n<tr>\n<td>RDB14530<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14530\">pFBD-Hs alpha1 CFXaGH\/beta3 CFXaGF<\/a><\/td>\n<td>Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3.<\/td>\n<\/tr>\n<tr>\n<td>RDB14531<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14531\">pFBD-Hs alpha1(K40R) CFXaGH\/beta3 CFXaGF<\/a><\/td>\n<td>Expression vector of His tagged human tubulin alpha-1(mutant, K40R, constitutive active form) and Flag tagged human tubulin beta-3.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">Expression of heterologous proteins in Drosophila Schneider S2 (S2)<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0048e#insect\">Empty Backbone for Insect Cell Experiment<\/a><\/td>\n<td>A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins.<br \/>\nIwaki, T., Castellino, F.J.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=19003171\">Cytotechnology 57 (1), 45-49, (2008). PubMed PMID 19003171.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13444\">Chinese hamster genomic BAC library clone<\/a><\/td>\n<td>Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells.<br \/>\nCao, Y., Kimura, S., Itoi, T., Honda, K., Ohtake, H., Omasa, T.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=22100493\">Methods 56 (3): 418-423 (2012). PubMed PMID 22100493.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">Visualization of sphingolipids<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GSB0027e\">Genes involved in the sphingolipid signaling pathway<\/a><\/td>\n<td>Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis.<br \/>\nAbe, M., Kobayashi, T.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=28065303\">Methods Cell Biol. 137: 15-24  (2017). PubMed PMID 28065303.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">Optogenetic technology using cultured cell lines<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0057e\">Genetic Resources for Optgenetics Research<\/a><\/td>\n<td>Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells.<br \/>\nAsano, T., Ishizuka, T., Yawo, H.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=28842907\">Methods Mol. Biol. 1668: 135-145 (2017). PubMed PMID 28842907.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<li><span style=\"text-decoration:underline;\">Cell-free protein synthesis with noncanonical amino acids using Escherichia coli cell extract<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0028e#grp0028e\">E. coli strain for producing recombinant proteins incorporating synthetic amino acids<\/a><\/td>\n<td>Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains.<br \/>\nSeki, E., Yanagisawa, T., Yokoyama, S.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=29404990\">Methods Mol. Biol. 1728: 49-65 (2018). PubMed PMID 29404990.<\/a><\/td>\n<\/tr>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">Cell cycle observation by using Fluorescence Ubiquitination Cell Cycle Indicator (Fucci)<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0044e\">Fucci &#8211; Fluorescent Ubiquitination-based Cell Cycle Indicator<\/a><\/td>\n<td>Methods to study the proliferation and differentiation of cardiac side population (CSP) cells.<br \/>\nSereti, K.I., Oikonomopoulos, A., Unno, K., Liao, R.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=23807790\">Methods Mol. Biol.  1036: 95-106 (2013). PubMed PMID 23807790.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">Transduction of gene to fission yeast by pDUAL and pDUAL2 vectors<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0027e\">Expression Vector Backbone of S. pombe<\/a><\/td>\n<td>Heterologous gene expression by chromosomal integration in fission yeast.<br \/>\nMatsuyama, A., Yoshida, M.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=22160913\">Methods Mol. Biol. 824: 433-450 (2012). PubMed PMID 22160913.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration:underline;\">E. coli-based in-cell NMR experiment<\/span><br \/>\n<table>\n<tbody>\n<tr>\n<th>Resource page<\/th>\n<th>Protocol<\/th>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0051e\">Thermus thermophilus Expression plasmid<\/a><\/td>\n<td>In-cell NMR of intrinsically disordered proteins in prokaryotic cells.<br \/>\nIto, Y., Mikawa, T., Smith, B.O.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=22760309\">Methods Mol. Biol., 895: 19-31 (2012). PubMed PMID 22760309.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#top\">go to top<\/a><\/p>\n<hr \/>\n<p><a name=\"reference\"><\/a><\/p>\n<h3>Reference of Genetic Materials<\/h3>\n<ul style=\"line-height: 1.5em;\">\n<li>List of <a href=\"\/en\/manualen\/references_en\">references of genetic materials<\/a> (promoters, plasmid vectors and so on).<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0065j\">\u5229\u7528\u8005\u306b\u3088\u308b\u5b66\u8853\u8ad6\u6587\u306e\u6210\u679c<\/a><br \/>\n\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u3088\u308a\u5165\u624b\u3057\u305f\uff24\uff2e\uff21\u3084\u30a6\u30a4\u30eb\u30b9\u682a\u3092\u7528\u3044\u3066\u6210\u679c\u3092\u8ad6\u6587\u767a\u8868\u3055\u308c\u308b\u5834\u5408\u3001\u5fc5\u305a\u7406\u7814BRC\u306a\u3089\u3073\u306b\u6587\u90e8\u79d1\u5b66\u7701\u30ca\u30b7\u30e7\u30ca\u30eb\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3078\u306e\u8b1d\u8f9e (Acknowledgement) \u3092Acknowledgement\u6b04\u3084Materials &#038; Methods\u6b04\u306b\u8a18\u8f09\u3057\u3066\u9802\u304f\u3088\u3046\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<\/li>\n<li><a href=\"https:\/\/web.brc.riken.jp\/ja\/method\/anthology\"> \u30d7\u30ed\u30c8\u30b3\u30eb\u3001\u30de\u30cb\u30e5\u30a2\u30eb (\u7406\u7814BRC \u30a6\u30a7\u30d6\u30b5\u30a4\u30c8) [link]<\/a><\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a href=\"#top\">go to top<\/a><\/p>\n<hr \/>\n<p style=\"text-align: right;\">(MAN0000j 2016.06.21 T.M.)<\/p>\n<p style=\"text-align: left;\">2021.03.29<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5927\u8178\u83cc\u306e\u53d6\u6271\u3044 \u30d7\u30e9\u30b9\u30df\u30c9\u3001\u30b3\u30b9\u30df\u30c9\u3001BAC\u30af\u30ed\u30fc\u30f3 \u30b2\u30ce\u30e0DNA \u54fa\u4e73\u52d5\u7269\u57f9\u990a\u7d30\u80de \u7d44\u63db\u3048\u30a6\u30a4\u30eb\u30b9 Technical notes \u5bc4\u8a17\u3055\u308c\u305f\u907a\u4f1d\u5b50\u6750\u6599\u3092\u7528\u3044\u305f\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb Reference of Genetic Materials \u5927\u8178\u83cc\u306e\u53d6\u6271\u3044 \u5927\u8178\u83cc\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb \u5927\u8178\u83cc\u7d44\u63db\u3048\u86cb\u767d\u8cea\u306e\u8abf\u88fd \u30b9\u30bf\u30d6\u5bd2\u5929\u304b\u3089\u306e\u83cc\u306e\u57f9\u990a \u767d\u91d1\u8033\u3067\u30b9\u30bf\u30d6\u5bd2\u5929\u306e\u83cc\u306e\u751f\u3048\u3066\u3044\u308b\u3068\u3053\u308d\u306b\u7a81\u304d\u523a\u3057\u3066\u83cc\u3092\u3068\u308a\u3001\u5bd2\u5929\u30d7\u30ec [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-296","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/296","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=296"}],"version-history":[{"count":20,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/296\/revisions"}],"predecessor-version":[{"id":5188,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/296\/revisions\/5188"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=296"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}