{"id":2403,"date":"2009-06-13T13:54:26","date_gmt":"2009-06-13T04:54:26","guid":{"rendered":"http:\/\/dna.brc.riken.jp:8002\/?page_id=2403"},"modified":"2018-10-22T09:37:16","modified_gmt":"2018-10-22T00:37:16","slug":"manual_05","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/manual\/manual_05","title":{"rendered":"\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u53d6\u6271\u3044"},"content":{"rendered":"<h3>\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5897\u5e45\u306b\u3064\u3044\u3066<\/h3>\n<p>\u25a0\u3000\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5897\u3048\u305f\u308a\u5897\u3048\u306a\u304b\u3063\u305f\u308a\u3068\u3044\u3046\u7d4c\u9a13\u306f\u3042\u308a\u307e\u305b\u3093\u304b\uff1f3 mL \u306e\u5c11\u91cf\u57f9\u990a\u306a\u3089DNA \u304c\u3068\u308c\u308b\u306e\u306b\u3001\u305d\u308c\u3092\u5927\u91cf\u57f9\u990a\u3059\u308b\u3068\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u53ce\u91cf\u304c\u3068\u3066\u3082\u5c11\u306a\u3044\u3002\u540c\u3058\u554f\u984c\u306f\u3001\u3042\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u30af\u30ed\u30fc\u30f3\u306eovernight culture \u3092\u57f9\u990a\u3057\u76f4\u3057\u305f\u6642\u306b\u8d77\u3053\u308a\u307e\u3057\u305f\u3002\u5168\u304fDNA \u304c\u7121\u3044\u308f\u3051\u3067\u306f\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u5927\u91cf\u57f9\u990a\u306b\u3057\u3066\u306f\u91cf\u304c\u5c11\u306a\u3044\u3002\u3057\u304b\u3057\u3001overnight culture \u305d\u306e\u3082\u306e\u3067\u306f\u30d7\u30e9\u30b9\u30df\u30c9DNA \u304c\u3042\u308b\u7a0b\u5ea6\u306f\u53d6\u308c\u3066\u3044\u308b\u3002\u524d\u57f9\u990a\u3092\u3059\u308b\u3068DNA \u306e\u53ce\u91cf\u304c\u6e1b\u308b\u3002\u305d\u308c\u306f\u306a\u305c\u304b\uff1f\u3082\u3057\u304b\u3057\u305f\u3089\u3001\u3044\u3063\u305f\u3093\u83cc\u304c\u5897\u3048\u304d\u3063\u305f\u3089\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u8131\u843d\u3057\u3066\u3044\u304f\u306e\u3067\u306f\u306a\u3044\u3060\u308d\u3046\u304b\uff1f<br \/>\n\u25a0\u3000\u305d\u3046\u8003\u3048\u3066\u3001log phase \u3067\u5897\u3084\u3057\u7d9a\u3051\u308c\u3070\u554f\u984c\u306f\u8d77\u304d\u306a\u3044\u306e\u3067\u306f\u306a\u3044\u3060\u308d\u3046\u304b\u3001\u3068\u3044\u3046\u524d\u63d0\u306e\u3082\u3068\u3067\u5b9f\u9a13\u3057\u307e\u3057\u305f\u3002<\/p>\n<ul>\n<li>08:00\u3001\u30d7\u30ec\u30fc\u30c8\u306esingle colony \u30924 mL \u306e\u57f9\u5730\u306b\u690d\u3048\u4ed8\u3051\u307e\u3057\u305f\u3002<\/li>\n<li>12:00\u3001\u305d\u3053\u304b\u3089100 ul \u305a\u30645\u672c\u306e2 mL \u57f9\u5730\u306b\u690d\u3048\u307e\u3057\u305f\uff08\u524d\u57f9\u990a\uff09\u3002<\/li>\n<li>13:30\u304b\u30891\u6642\u9593\u304a\u304d\u306b\u3001\u305d\u308c\u305e\u308c\u304b\u3089100 ul \u53d6\u308a\u30012 mL \u306e\u57f9\u5730\u306b\u690d\u3048\u307e\u3057\u305f\uff08\u672c\u57f9\u990a\uff09\u3002\u3000\u524d\u57f9\u990a3.5\u6642\u9593\u3067\u83cc\u306e\u5897\u6b96\u306f\u6700\u5927\u306b\u9054\u3057\u307e\u3057\u305f\u3002<br \/>\n\u690d\u3048\u4ed8\u3051\u305f\u6642\u523b\u3068\u305d\u306e\u6642\u306eOD \u306f\u3001<br \/>\n\uff11\u300013:30\u30000.255<br \/>\n\uff12\u300014:30\u30000.680<br \/>\n\uff13\u300015:30\u30000.792<br \/>\n\uff14\u300016:30\u30000.840<br \/>\n\uff15\u300017:30\u30000.799<\/li>\n<li>23:30\u3001\u305d\u308c\u305e\u308c\u304b\u3089DNA \u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002\uff08\u30b2\u30eb\u30a4\u30e1\u30fc\u30b8\u53c2\u7167\uff09<\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"NWL050730\" src=\"\/\/dnaconda.riken.jp\/images\/nwl050730.gif\" width=\"300\" \/><\/a><br \/>\n\u25a0\u3000\u524d\u57f9\u990a\u306e\u5897\u6b96\u304c\u6700\u5927\u306b\u9054\u3057\u305f\u83cc\uff08\uff14\u3068\uff15\uff09\u3092\u690d\u3048\u4ed8\u3051\u305f\u5834\u5408\u306f\u3001\u5897\u6b96\u9014\u4e2d\u306e\u83cc\u3092\u690d\u3048\u4ed8\u3051\u305f\u5834\u5408\uff08\uff11\u304b\u3089\uff13\uff09\u306b\u6bd4\u3079\u3001DNA \u306e\u53ce\u91cf\u304c\u5c11\u306a\u304f\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u3068\u304b\u3089\u3001\u5897\u6b96\u3057\u304d\u3063\u305f\u524d\u57f9\u990a\u3092\u690d\u3048\u4ed8\u3051\u305f\u5834\u5408\u306f\u3001DNA \u306e\u53ce\u91cf\u304c\u5c11\u306a\u304f\u306a\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002<br \/>\n\u25a0\u3000DNA \u8abf\u88fd\u306e\u305f\u3081\u306e\u5927\u8178\u83cc\u57f9\u990a\u6642\u9593\u306b\u9055\u3044\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u4e00\u756a\u9045\u304f\u690d\u3048\u4ed8\u3051\u305f\u5834\u5408\u3067\u30826\u6642\u9593\u306e\u57f9\u990a\u6642\u9593\u304c\u3042\u308a\u307e\u3059\u300212:00\u306b\u306f\u3058\u3081\u305f\u524d\u57f9\u990a\u306f\u3001\u672c\u57f9\u990a\u3068\u540c\u3058\u304f100 ul \u30922 mL \u306e\u57f9\u5730\u306b\u690d\u3048\u3066\u3044\u307e\u3059\u304c\u30013.5\u6642\u9593\u3067\u83cc\u306e\u5897\u6b96\u304c\u6700\u5927\u306b\u9054\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u30016\u6642\u9593\u306e\u57f9\u990a\u6642\u9593\u306f\u3001\u83cc\u306e\u5897\u6b96\u306b\u5341\u5206\u306a\u6642\u9593\u3092\u7d4c\u904e\u3057\u3066\u3044\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u5f93\u3063\u3066\u3001\u83cc\u306e\u5897\u6b96\u306e\u5dee\u304cDNA \u306e\u53ce\u91cf\u306b\u53cd\u6620\u3055\u308c\u305f\u308f\u3051\u3067\u306f\u306a\u3044\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<br \/>\n\u25a0\u3000\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5927\u8178\u83cc\u304b\u3089\u8abf\u88fd\u3059\u308b\u5834\u5408\u3001\u83cc\u306f\u5897\u6b96\u3055\u305b\u904e\u304e\u305a\u306bDNA \u3092\u8abf\u88fd\u3059\u308b\u3053\u3068\u304c\u5927\u5207\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u3082\u3061\u308d\u3093\u5168\u3066\u306e\u5834\u5408\u306b\u3042\u3066\u306f\u307e\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u3044\u3064\u3082\u3046\u307e\u304f\u884c\u304fDNA \u53d6\u308a\u304c\u6025\u306b\u3046\u307e\u304f\u884c\u304b\u306a\u304f\u306a\u3063\u305f\u5834\u5408\u306b\u306f\u3001\u3053\u306e\u304a\u8a71\u3092\u601d\u3044\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002(TM)<br \/>\n(Mail News 2005.07.30 \u63b2\u8f09\u8a18\u4e8b\u3088\u308a)<\/p>\n<h3>\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5897\u5e45\u306b\u3064\u3044\u3066 (2)<\/h3>\n<p>\u25a0 \u5165\u624b\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9 DNA \u3092\u5f62\u8cea\u8ee2\u63db\u3057\u305f\u83cc\u304c\u5897\u3048\u306a\u3044\u3001\u3068\u3044\u3046\u76f8\u8ac7\u3092\u53d7\u3051\u307e\u3057\u305f\u3002\u3053\u3046\u3044\u3046\u6642\u306b\u306f\u3001\u305f\u3044\u3066\u3044\u3001\u305d\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5bbf\u4e3b\u3068\u3057\u3066\u672c\u6765\u6307\u5b9a\u3055\u308c\u3066\u3044\u308b\u5927\u8178\u83cc\u7cfb\u7d71\u4ee5\u5916\u306e\u7cfb\u7d71\u306b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5c0e\u5165\u3057\u305f\u304b\u3001\u3042\u308b\u3044\u306f\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u85ac\u5264\u8010\u6027\u907a\u4f1d\u5b50\u3092\u9593\u9055\u3063\u3066\u672c\u6765\u3067\u306a\u3044\u6297\u751f\u7269\u8cea\u3092\u542b\u3080\u57f9\u5730\u3067\u57f9\u990a\u3057\u305f\u5834\u5408\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u672c\u6765\u306e\u6b63\u3057\u3044\u6297\u751f\u7269\u8cea\u3092\u542b\u3080\u57f9\u5730\u3067\u3042\u3063\u3066\u3082\u3001\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3068 DNA \u3092\u6df7\u305c\u305f\u5f8c\u3001\u5bd2\u5929\u57f9\u5730\u306b\u64ad\u304f\u524d\u306b\u6297\u751f\u7269\u8cea\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u4e2d\u3067 30 \u5206\u7a0b\u5ea6\u57f9\u990a\u3057\u306a\u3044\u3068\u3001\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u304c\u4f4e\u4e0b\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u3088\u3046\u306a\u53ef\u80fd\u6027\u304c\u306a\u3044\u304b\u305f\u305a\u306d\u307e\u3057\u305f\u3068\u3053\u308d\u3001\u6297\u751f\u7269\u8cea\u306f\u6b63\u3057\u304f\u4f7f\u3063\u3066\u3044\u308b\u3057\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u305f\u3081\u306e\u7279\u6b8a\u306a\u83cc\u682a\u306e\u6307\u5b9a\u3082\u306a\u3044\u3002\u83cc\u306f\u3061\u3083\u3093\u3068\u751f\u3048\u3066\u3044\u308b\u3001\u3068\u3044\u3046\u7b54\u3048\u3067\u3059\u3002<br \/>\n\u25a0 \u3042\u308c\u3042\u308c\uff1f\u6700\u521d\u306e\u8cea\u554f\u306f\u83cc\u304c\u5897\u3048\u306a\u3044\u3001\u3068\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u3067\u3001\u83cc\u304c\u751f\u3048\u306a\u3044\u306e\u304b\u3068\u601d\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u83cc\u306f\u751f\u3048\u3066\u3044\u308b\u3001\u3068\u8a00\u3063\u3066\u3044\u308b\u306e\u3060\u304b\u3089\u3001\u3082\u3057\u304b\u3057\u305f\u3089\u3001\u83cc\u306f\u5897\u3048\u308b\u306e\u306b\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u53ce\u91cf\u304c\u5c11\u306a\u3044\u3001\u3068\u8a00\u3044\u305f\u3044\u306e\u3060\u308d\u3046\u304b\u3002\u305d\u3046\u601d\u3044\u3001\u30d0\u30f3\u30af\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u3001\u30e9\u30dc\u30de\u30cb\u30e5\u30a2\u30eb\u306e Technical notes #01 \u306e\u300c\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u57f9\u990a\u306b\u3064\u3044\u3066\u300d\u3092\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u305d\u3053\u306b\u306f\u3001\u300c\u524d\u57f9\u990a\u304c\u9577\u3044\u3068\u3001\u83cc\u306f\u5897\u6b96\u3057\u3066\u3082\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5897\u5e45\u3055\u308c\u306a\u3044\u300d\u3068\u3044\u3046\u3053\u3068\u304c\u66f8\u304b\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u25a0 \u3068\u3053\u308d\u304c\u3001\u3053\u308c\u3082\u9055\u3046\u3068\u3044\u3046\u3053\u3068\u3067\u3057\u305f\u3002\u3064\u307e\u308a\u3001\u5165\u624b\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9 DNA \u3067\u5f62\u8cea\u8ee2\u63db\u3057\u305f\u76f4\u5f8c\u306e\u5927\u8178\u83cc\u306f\u3001\u5bd2\u5929\u57f9\u5730\u306e\u4e0a\u3067\u30b3\u30ed\u30cb\u30fc\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u3001\u3068\u3044\u3046\u3053\u3068\u3002\u554f\u984c\u306f\u305d\u306e\u3042\u3068\u3067\u3001\u30b3\u30ed\u30cb\u30fc\u3092\u30d4\u30c3\u30af\u30a2\u30c3\u30d7\u3057\u3001\u6db2\u4f53\u57f9\u990a\u3067\u5927\u8178\u83cc\u3092\u5927\u91cf\u306b\u5f97\u3088\u3046\u3068\u3057\u3066\u3082\u3001\u83cc\u304c\u5168\u304f\u5897\u3048\u3066\u3044\u306a\u3044\u3001\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u305d\u308c\u306a\u3089\u3070\u3001\u6297\u751f\u7269\u8cea\u3092\u9593\u9055\u3048\u3066\u3044\u308b\u304b\u3001\u6297\u751f\u7269\u8cea\u306e\u6fc3\u5ea6\u304c\u6fc3\u3059\u304e\u308b\u304b\uff1f\u3042\u308b\u3044\u306f\u3001\u5bd2\u5929\u57f9\u5730\u4e0a\u3067\u306f\u751f\u3048\u3066\u6db2\u4f53\u57f9\u5730\u3067\u306f\u751f\u3048\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u306f\u3001\u6db2\u4f53\u57f9\u990a\u3067\u30a8\u30a2\u30fc\u30ec\u30fc\u30b7\u30e7\u30f3\u304c\u4e0a\u624b\u304f\u3044\u3063\u3066\u3044\u306a\u3044\u306e\u304b\uff1f\u305d\u3046\u3044\u3046\u53ef\u80fd\u6027\u3092\u8003\u3048\u3066\u30012 mL\u57f9\u990a\u3067\u3001\u6297\u751f\u7269\u8cea\u306e\u6fc3\u5ea6\u3092\u5909\u3048\u3066\u4f55\u672c\u304b\u57f9\u990a\u3057\u3066\u306f\u3069\u3046\u304b\u3001\u3068\u52e7\u3081\u307e\u3057\u305f\u3002<br \/>\n\u25a0 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\/>\n\uff11\uff0e\u5165\u624b\u3057\u305f\uff24\uff2e\uff21\u3067\u5927\u8178\u83cc\u3092\u5f62\u8cea\u8ee2\u63db\u3057\u3001\u5bd2\u5929\u57f9\u5730\u4e0a\u306b\u751f\u3084\u3059\u3002<br \/>\n\uff12\uff0e\u305d\u3053\u306b TE \u3042\u308b\u3044\u306f PBS \u3092\u305f\u3089\u3057\u3001\u5bd2\u5929\u57f9\u5730\u4e0a\u306e\u83cc\u3092\u56de\u53ce\u3059\u308b\u3002<br \/>\n\uff13\uff0e\u56de\u53ce\u3057\u305f\u83cc\u304b\u3089\u3001\u901a\u5e38\u901a\u308a\u30d7\u30e9\u30b9\u30df\u30c9\uff24\uff2e\uff21\u3092\u8abf\u88fd\u3059\u308b\u3002<br \/>\n\uff14\uff0e\u305d\u306e\u8abf\u88fd\u3057\u305f\uff24\uff2e\uff21\u3092\u3055\u3089\u306b\u30d5\u30a7\u30ce\u30fc\u30eb\u62bd\u51fa\u3057\u3066\u7cbe\u88fd\u3059\u308b\u3002<br \/>\n\uff15\uff0e\u305d\u306e\u7cbe\u88fd\u3057\u305f\uff24\uff2e\uff21\u3067\u65b0\u305f\u306b\u5f62\u8cea\u8ee2\u63db\u3057\u305f\u5927\u8178\u83cc\u3092\u5bd2\u5929\u57f9\u5730\u4e0a\u306b\u751f\u3084\u3059\u3002<br \/>\n\uff16\uff0e\u5bd2\u5929\u57f9\u5730\u4e0a\u306e\u30b3\u30ed\u30cb\u30fc\u304b\u3089\u6db2\u4f53\u57f9\u990a\u3059\u308b\u3002<br \/>\n\u25a0 \u3053\u306e\u3084\u308a\u65b9\u306f\u3001\u898b\u4e8b\u306b\u7684\u3092\u5f97\u3001\u7121\u4e8b\u3001\u30d7\u30e9\u30b9\u30df\u30c9\uff24\uff2e\uff21\u3092\u5927\u91cf\u8abf\u88fd\u3067\u304d\u305f\u305d\u3046\u3067\u3059\u3002(T.M.)<br \/>\n(Mail News 2007.08.04 \u63b2\u8f09\u8a18\u4e8b\u3088\u308a)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5897\u5e45\u306b\u3064\u3044\u3066 \u25a0\u3000\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5897\u3048\u305f\u308a\u5897\u3048\u306a\u304b\u3063\u305f\u308a\u3068\u3044\u3046\u7d4c\u9a13\u306f\u3042\u308a\u307e\u305b\u3093\u304b\uff1f3 mL \u306e\u5c11\u91cf\u57f9\u990a\u306a\u3089DNA 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