{"id":111,"date":"2013-02-25T15:59:44","date_gmt":"2013-02-25T06:59:44","guid":{"rendered":"http:\/\/vps36.rbrc.jp:8213\/?page_id=111"},"modified":"2024-01-23T08:36:26","modified_gmt":"2024-01-22T23:36:26","slug":"journalclub","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/ja\/news_archives\/journalclub","title":{"rendered":"Dnaconda\u306e\u304a\u3059\u3059\u3081"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<div style=\"text-align:right;\"><img loading=\"lazy\" decoding=\"async\" title=\"dnaconda\" src=\"https:\/\/dnaconda.riken.jp\/images\/dnaconda01.png\" alt=\"\" width=\"100\" height=\"68\" \/>\n<\/div>\n<p><a name=\"EXR0114j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> HIV-1\u611f\u67d3\u7d30\u80de\u3092\u7279\u5fb4\u3065\u3051\u308b\u907a\u4f1d\u5b50\u767a\u73fe (2021\/11\/01 T.M.)<script>newImage(2021,11,01)<\/script>\n<ul style=\"line-height: 1.5em;\">\n<li>\u514d\u75ab\u306e\u53f8\u4ee4\u5854\u306e\u5f79\u5272\u3092\u679c\u305f\u3059\u30d2\u30c8 CD4 T\u7d30\u80de\u306f\u3001HIV-1\u306e\u4e3b\u305f\u308b\u611f\u67d3\u6a19\u7684\u3067\u3059\u3002\u672c\u8ad6\u6587\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001CD4 T\u7d30\u80de\u3092\u542b\u3080\u4eba\u306e\u514d\u75ab\u30b7\u30b9\u30c6\u30e0\u3092\u518d\u69cb\u7bc9\u3057\u305f\u300c\u30d2\u30c8\u5316\u30de\u30a6\u30b9\u300d\u306b\u30ec\u30dd\u30fc\u30bf\u30fc\u30a6\u30a4\u30eb\u30b9HIV1-GFP\u3092\u611f\u67d3\u3055\u305b\u3001\u7d30\u80de\u306e\u591a\u89d2\u7684\u89e3\u6790\u624b\u6cd5\u306b\u3088\u308aHIV-1\u7523\u51fa\u7d30\u80de\u3092\u7279\u5fb4\u3065\u3051\u308b\u8981\u56e0\u3068\u3057\u3066\u3001CXCL13\u907a\u4f1d\u5b50\u9ad8\u767a\u73fe\u96c6\u56e3\u3068\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30ed\u30f3\u8a98\u5c0e\u907a\u4f1d\u5b50\u306e\u4f4e\u767a\u73fe\u96c6\u56e3\u304c\u3042\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u30a8\u30a4\u30ba\u6839\u6cbb\u7642\u6cd5\u306b\u5411\u3051\u3066\u5fc5\u9808\u306aHIV-1\u6f5c\u4f0f\u611f\u67d3\u7d30\u80de\u306e\u7279\u5fb4\u306e\u7406\u89e3\u3092\u6df1\u3081\u308b\u3053\u3068\u306b\u5f79\u7acb\u3064\u3068\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/li>\n<li>CXCL13\u306e\u53d7\u5bb9\u4f53\u3067\u3042\u308bCXCR5\u3092\u767a\u73fe\u3059\u308b\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u4f5c\u88fd\u306bCSII-CMV-MCS-IRES2-Bsd (cat# RDB04385)\u3001pCMV-VSV-G-RSV-Rev (cat# RDB04393)\u3001pCAG-HIVgp (cat# RDB04394)\u304c\u7528\u3044\u3089\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\n\tAso, H. et al., Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo. Cell Rep. 32 (2): 107887, 2020. PMID: 32668246.<\/li>\n<li>\u30d7\u30ec\u30b9\u767a\u8868<br \/>\n\t\u6771\u4eac\u5927\u5b66\u3001\u65e5\u672c\u533b\u7642\u7814\u7a76\u958b\u767a\u6a5f\u69cb\u300c\u751f\u4f53\u5185\u306b\u304a\u3051\u308bHIV-1\u611f\u67d3\u7d30\u80de\u306e\u30de\u30eb\u30c1\u30aa\u30df\u30af\u30b9\u89e3\u6790 &#8211; \u30a8\u30a4\u30ba\u6839\u6cbb\u6cd5\u306e\u624b\u304c\u304b\u308a\u63a2\u7d22\u306b\u9053 &#8211; \u300d<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB04385\">CSII-CMV-MCS-IRES2-Bsd (cat# RDB04385)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB04393\">pCMV-VSV-G-RSV-Rev (cat# RDB04393)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB04394\">pCAG-HIVgp (cat# RDB04394)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0116j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u65b0\u751f\u5150\u306e\u91cd\u7be4\u306a\u5fc3\u7b4b\u75c7\u306e\u539f\u56e0\u907a\u4f1d\u5b50\u3092\u5927\u898f\u6a21\u89e3\u6790 (2021\/10\/26 T.M.)<script>newImage(2021,10,26)<\/script>\n<ul style=\"line-height: 1.5em;\">\n<li>\u672c\u8ad6\u6587\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u65b0\u751f\u5150\u306e\u91cd\u7be4\u306a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u5fc3\u7b4b\u75c7\u3092\u5448\u3059\u308b\u65e5\u672c\u4eba\u75c7\u4f8b\u306e\u30b2\u30ce\u30e0\u89e3\u6790\u306b\u3088\u308a\u3001ATAD3\u907a\u4f1d\u5b50\u30af\u30e9\u30b9\u30bf\u30fc\u306e\u91cd\u8907\u304c\u767a\u75c7\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u56fd\u969b\u5171\u540c\u7814\u7a76\u306b\u3088\u308b\u5927\u898f\u6a21\u89e3\u6790\u3092\u884c\u3044\u300116\u5bb6\u7cfb\u3067\u540c\u69d8\u306e\u91cd\u8907\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002ATAD3\u907a\u4f1d\u5b50\u306e\u91cd\u8907\u306b\u95a2\u3057\u3066\u306f\u3001\u7570\u5e38\u306a\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u75be\u60a3\u767a\u75c7\u306b\u5bc4\u4e0e\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3053\u3068\u3092\u793a\u5506\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>ATAD3\u8907\u5408\u4f53\u5f62\u6210\u306e\u7570\u5e38\u3068\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u6a5f\u80fd\u7570\u5e38\u306e\u89e3\u6790\u3067\u306f\u3001ATAD3A, ATAD3C, \u30ad\u30e1\u30e9ATAD3A\/ATAD3C\u907a\u4f1d\u5b50\u3092\u904e\u5270\u767a\u73fe\u3059\u308b\u7d30\u80de\u682a\u3092CS-CA-MCS (cat# RDB05963)\u3092\u4f7f\u3063\u3066\u6a39\u7acb\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\n\tFrazier, A.E. et al., Fatal perinatal mitochondrial cardiac failure caused by recurrent de novo duplications in the ATAD3 locus. Med (N Y) 2 (1): 49-73, 2021. PMID: 33575671.<\/li>\n<li>\u30d7\u30ec\u30b9\u767a\u8868<br \/>\n\t\u5343\u8449\u770c\u3053\u3069\u3082\u75c5\u9662\u307b\u304b \u300c\u65b0\u751f\u5150\u306e\u91cd\u7be4\u306a\u5fc3\u7b4b\u75c7\u3092\u8d77\u3053\u3059\u907a\u4f1d\u5b50\u3092\u56fd\u969b\u9023\u643a\u3067\u5927\u898f\u6a21\u306a\u5831\u544a &#8211; \u5fc3\u7b4b\u75c7\u306e\u65e9\u671f\u8a3a\u65ad\u3068\u6cbb\u7642\u6cd5\u958b\u767a\u3078\u671f\u5f85 &#8211; \u300d 2020\u5e747\u670810\u65e5\u3002<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05963\">CS-CA-MCS (cat# RDB05963)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0115j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u8907\u6570\u306e\u30a6\u30a4\u30eb\u30b9\u306b\u6297\u30a6\u30a4\u30eb\u30b9\u6d3b\u6027\u3092\u793a\u3059\u85ac\u5264\u306e\u767a\u898b (2021\/10\/20 T.M.)<script>newImage(2021,10,20)<\/script>\n<ul style=\"line-height: 1.5em;\">\n<li>\u4e16\u754c\u3067\u306f\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u75c7\u304c\u6d41\u884c\u3057\u3066\u304a\u308a\u3001\u6cbb\u7642\u85ac\u306e\u958b\u767a\u304c\u55ab\u7dca\u306e\u8ab2\u984c\u3067\u3059\u3002\u672c\u8ad6\u6587\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u30015-Hydroxymethyltubercidin (HMTU) \u304c\u8907\u6570\u306e\u30a6\u30a4\u30eb\u30b9\u7a2e\u306b\u5bfe\u3057\u3066\u3001\u6297\u30a6\u30a4\u30eb\u30b9\u6d3b\u6027\u3092\u793a\u3059\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u884c\u3063\u305f\u89e3\u6790\u306e\u3046\u3061\u3001\u30d2\u30c8\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\u304c\u611f\u67d3\u3057\u305f\u7d30\u80de\u3067\u306e\u5897\u6b96\u3078\u306e\u5f71\u97ff\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u3001SARS-CoV-2\u3067\u306fVeroE6\u7d30\u80de\u682a\u3001HCoV-OC43\u3067\u306fA549\u7d30\u80de\u682a\u3092\u3082\u3068\u306b\u3057\u305fTMPRSS2\u3092\u6052\u5e38\u767a\u73fe\u3059\u308b\u7d30\u80de\u3092\u6a39\u7acb\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u7d30\u80de\u6a39\u7acb\u306e\u305f\u3081\u306b\u4f7f\u7528\u3057\u305fTMPRSS2\u3092\u767a\u73fe\u3059\u308b\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9\u306b\u3001CSII-CMV-MCS-IRES2-Bsd (cat# RDB04385)\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nUemura, K. et al., 5-Hydroxymethyltubercidin exhibits potent antiviral activity against flaviviruses and coronaviruses, including SARS-CoV-2. iScience. 24 (10): 103120, 2021. PMID: 34541466.<\/li>\n<li>\u30d7\u30ec\u30b9\u767a\u8868<br \/>\n\u5317\u6d77\u9053\u5927\u5b66\u3001\u65e5\u672c\u533b\u7642\u7814\u7a76\u958b\u767a\u6a5f\u69cb\u300c\u65b0\u578b\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u6b96\u3092\u6291\u3048\u308b\u6838\u9178\u4ee3\u8b1d\u62ee\u6297\u85ac\u306e\u767a\u898b\uff5e\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9\u3084\u30d5\u30e9\u30d3\u30a6\u30a4\u30eb\u30b9\u7b49\uff0c\u5e83\u57df\u7684\u6297\u30a6\u30a4\u30eb\u30b9\u85ac\u3068\u3057\u3066\u306e\u671f\u5f85\uff5e\u300d2021\u5e749\u670814\u65e5\u3002<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB04385\">CSII-CMV-MCS-IRES2-Bsd (cat# RDB04385)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0113j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u7d44\u63db\u3048\u30ac\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3068\u7cbe\u88fd (2021\/05\/13 N.N.)<script>newImage(2021,5,13)<\/script>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30ac\u30ec\u30af\u30c1\u30f3\u306f\u3001\u03b2\u30ac\u30e9\u30af\u30c8\u30b7\u30c9\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u30ec\u30af\u30c1\u30f3\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u7dcf\u79f0\u3067\u3042\u308a\u3001\u767a\u751f\u3001\u5206\u5316\u3001\u764c\u3001\u514d\u75ab\u306a\u3069\u5e45\u5e83\u3044\u751f\u547d\u73fe\u8c61\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u9577\u5e74\u30ac\u30ec\u30af\u30c1\u30f3\u7814\u7a76\u306b\u643a\u308f\u3063\u3066\u3053\u3089\u308c\u305f\u9999\u5ddd\u5927\u5b66\u306e\u897f \u671b \u5148\u751f\u306f\u3001\u3053\u308c\u307e\u3067\u306b\u5927\u8178\u83cc\u3092\u5229\u7528\u3057\u305f\u30d2\u30c8\u30ac\u30ec\u30af\u30c1\u30f3\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u767a\u73fe\u3068\u7cbe\u88fd\u65b9\u6cd5\u3092\u78ba\u7acb\u3055\u308c\u307e\u3057\u305f\u3002\u897f \u5148\u751f\u306f\u3001\u30ac\u30ec\u30af\u30c1\u30f3\u7814\u7a76\u3092\u3053\u308c\u304b\u3089\u59cb\u3081\u305f\u3044\u7814\u7a76\u8005\u306e\u4e00\u52a9\u3068\u306a\u308b\u3088\u3046\u306b\u3001\u7cd6\u9396\u5de5\u5b66\u5c02\u9580\u60c5\u5831\u30b5\u30a4\u30c8Glycoforum\uff08Glycoforum 23 (5), A15, 2000\uff09\u306b\u5927\u8178\u83cc\u3092\u7528\u3044\u3066\u767a\u73fe\u3055\u305b\u305f\u7d44\u63db\u3048\u30ac\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7cbe\u88fd\u306b\u95a2\u3059\u308b27\u30b9\u30c6\u30c3\u30d7\u304b\u3089\u306a\u308b\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u306f\u3001\u30b9\u30c6\u30c3\u30d7\u6bce\u306e\u64cd\u4f5c\u306e\u6ce8\u610f\u70b9\u3084\u5404\u30ac\u30ec\u30af\u30c1\u30f3\u306b\u95a2\u3059\u308b\u53d6\u308a\u6271\u3044\u306e\u6ce8\u610f\u70b9\u306a\u3069\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u30ac\u30ec\u30af\u30c1\u30f3\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u767a\u73fe\u306b\u7528\u3044\u308b\u5927\u8178\u83cc\u767a\u73fe\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u3001\u897f \u5148\u751f\u306e\u3054\u597d\u610f\u306b\u3088\u3063\u3066\u3001\u30d2\u30c8\u7d0460\u7a2e\u985e\u3001\u30de\u30a6\u30b9\u53ca\u3073\u30e9\u30c3\u30c8\u7d0420\u7a2e\u985e\u304c\u5f53\u5ba4\u306b\u5bc4\u8a17\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5bc4\u8a17\u3055\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u3001\u91ce\u751f\u578b\u306e\u4ed6\u3001\u7cd6\u9396\u7d50\u5408\u30b5\u30a4\u30c8\u306e\u4e00\u90e8\u3067Arg\u3092His\u306b\u7f6e\u63db\u3057\u305f\u5909\u7570\u4f53\u3001\u30ea\u30f3\u30ab\u30fc\u30da\u30d7\u30c1\u30c9\u306e\u4e00\u90e8\u3092\u9664\u53bb\u3057\u305f\u5909\u7570\u4f53\u306a\u3069\u6709\u7528\u306a\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3059\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u3001\u30ac\u30ec\u30af\u30c1\u30f3\u304c\u95a2\u4e0e\u3059\u308b\u751f\u547d\u73fe\u8c61\u306e\u7814\u7a76\u306e\u4e00\u52a9\u306b\u306a\u308c\u3070\u5e78\u3044\u3067\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\n<a href=\"https:\/\/www.glycoforum.gr.jp\/article\/23A15J.html\">\u897f \u671b\u3000\u7d44\u63db\u3048\u30ac\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3068\u7cbe\u88fd\u306b\u95a2\u3059\u308b\u30ce\u30fc\u30c8\u3000Glycoforum 23 (5), A15, 2000.<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GSB0048e\">Galectin family plasmid<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0112j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u30de\u30a4\u30c8\u30d5\u30a1\u30b8\u30fc\u53ef\u8996\u5316\u86cd\u5149\u30bb\u30f3\u30b5\u30fc mito-SRAI (2021\/05\/13 N.N.)<script>newImage(2021,5,13)<\/script>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30de\u30a4\u30c8\u30d5\u30a1\u30b8\u30fc\u306f\u3001\u640d\u50b7\u3057\u305f\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3092\u9078\u629e\u7684\u306b\u5206\u89e3\u3059\u308b\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3067\u3042\u308a\u3001\u4e0d\u826f\u306a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3092\u9664\u53bb\u3059\u308b\u7d30\u80de\u5185\u6a5f\u69cb\u3067\u3059\u3002<\/li>\n<li>\u3053\u306e\u5ea6\u3001\u7406\u5316\u5b66\u7814\u7a76\u6240\u8133\u795e\u7d4c\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u5bae\u8107\u6566\u53f2\u5148\u751f\u3001\u7247\u5c71\u535a\u5e78\u5148\u751f\u3068\u9806\u5929\u5802\u5927\u5b66\u306e\u65e5\u7f6e\u5bdb\u4e4b\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u751f\u304d\u305f\u7d30\u80de\u306e\u307f\u306a\u3089\u305a\u3001\u56fa\u5b9a\u6a19\u672c\u4e0a\u306b\u304a\u3044\u3066\u3082\u30de\u30a4\u30c8\u30d5\u30a1\u30b8\u30fc\u3092\u5b9a\u91cf\u7684\u306b\u53ef\u8996\u5316\u3067\u304d\u308b\u86cd\u5149\u30bb\u30f3\u30b5\u30fc\u3067\u3042\u308bmito-SRAI\u3092\u5171\u540c\u958b\u767a\u3057\u307e\u3057\u305f\u3002mito-SRAI\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u3068\u30ea\u30bd\u30bd\u30fc\u30e0\u5185\u306e\u9178\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306b\u8010\u6027\u3092\u6301\u3064\u30b7\u30a2\u30f3\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaTOLLES\u3068pH\u306b\u5fdc\u3058\u3066\u86cd\u5149\u5f37\u5ea6\u304c\u5909\u308f\u308b\u9ec4\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaYPet\u304b\u3089\u69cb\u6210\u3055\u308c\u307e\u3059\u3002mito-SRAI\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u5185\u90e8\u306b\u7279\u7570\u7684\u306b\u5c40\u5728\u3057\u3001\u304b\u3064\u56fa\u5b9a\u6a19\u672c\u3067\u306e\u89b3\u5bdf\u3067\u3082\u30b7\u30b0\u30ca\u30eb\u304c\u6d88\u5931\u3057\u306a\u3044\u3088\u3046\u306b\u5de5\u592b\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n<img decoding=\"async\" alt=\"\" width=\"600\" src=\"https:\/\/dnaconda.riken.jp\/images\/exr0112_900.png\" \/><\/li>\n<li>\u4eca\u56de\u958b\u767a\u3055\u308c\u305fmito-SRAI\u306f\u3001\u5275\u85ac\u30a2\u30c3\u30bb\u30a4\u306a\u3069\u81a8\u5927\u306a\u91cf\u306e\u56fa\u5b9a\u7d30\u80de\u6a19\u672c\u3092\u89b3\u5bdf\u3059\u308b\u5b9f\u9a13\u3084\u3001\u56fa\u5b9a\u7d44\u7e54\u6a19\u672c\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u52d5\u7269\u5b9f\u9a13\u306b\u3082\u5bfe\u5fdc\u3067\u304d\u307e\u3059\u3002\u57f9\u990a\u7d30\u80de\u306a\u3069\u306b\u767a\u73fe\u3055\u305b\u308b\u3053\u3068\u3067\u3001TOLLES \u3068YPet\u306e\uff12\u6ce2\u9577\u306e\u86cd\u5149\u5f37\u5ea6\u6bd4\u306b\u3088\u3063\u3066\u30de\u30a4\u30c8\u30d5\u30a1\u30b8\u30fc\u3092\u89b3\u5bdf\u3067\u304d\u307e\u3059 (Katayama, H. et al., Cell, 181 (5): 1176-1187, 2020. PMID: 32437660)\u3002<\/li>\n<li>\u8ad6\u6587\u3001\u95a2\u9023\u60c5\u5831<br \/>\nKatayama, H. et al. Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration.  Cell 181 (5): 1176-1187.e16 (2020). PubMed PMID 32437660<\/li>\n<li><a href=\"https:\/\/dna.brc.riken.jp\/en\/gsb0000en\/gsb0021en\">Resarch Tools of Autophagy and Mitophagy<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18222\">SRAI_pcDNA3 (cat# RDB18222)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18223\">mito-SRAI_pcDNA3 (cat# RDB18223)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18684\">pAAV2-TRE-mito-SRAI-BGHpA (cat# RDB18684)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0111j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u30b0\u30ea\u30aa\u30fc\u30de\u5f62\u6210\u306b\u5bfe\u3059\u308b\u9032\u5316\u7684\u306b\u4fdd\u5b58\u3055\u308c\u305f\u975e\u30b3\u30fc\u30c9RNA &#8220;ECONEXIN&#8221;\u306e\u767a\u304c\u3093\u306e\u52b9\u679c (2020\/11\/10 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u8fd1\u5e74\u3001lncRNA\u3068\u7d30\u80de\u304c\u3093\u5316\u306e\u95a2\u9023\u306b\u3064\u3044\u3066\u6ce8\u76ee\u3055\u308c\u59cb\u3081\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u307b\u3068\u3093\u3069\u306elncRNA\u306e\u6a5f\u80fd\u306f\u672a\u89e3\u6790\u3067\u3042\u308a\u3001\u65b0\u898f\u306e\u304c\u3093\u95a2\u9023lncRNA\u306e\u767a\u898b\u306f\u5bb9\u6613\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/li>\n<li>\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u6301\u3064\u907a\u4f1d\u5b50\u306e\u914d\u5217\u306f\u9032\u5316\u306e\u904e\u7a0b\u3067\u9ad8\u5ea6\u306b\u4fdd\u5b58\u6027\u3055\u308c\u3066\u3044\u308b\u3068\u306e\u4eee\u8aac\u306e\u3082\u3068\u3001\u7b46\u8005\u3089\u306f\u30d2\u30c8\u3068\u306e\u76f8\u540c\u6027\u304c\u9ad8\u3044\u30de\u30a6\u30b9\u306eC130071C03RIK\u907a\u4f1d\u5b50\u3068\u30d2\u30c8lncRNA\u907a\u4f1d\u5b50 &#8220;ECONEXIN&#8221; (LINC00461) \u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002CONEXIN\u306f\u3001\u8133\u3067\u3001\u3057\u304b\u3082\u30b0\u30ea\u30aa\u30fc\u30de\u304c\u3042\u308b\u6642\u306b\u7279\u306b\u767a\u73fe\u304c\u9ad8\u304f\u3001miR-411-5p\u3068\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u3001Argonaute (AGO2) \u3068\u8907\u5408\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002AGO2\u306fRNA\u4f9d\u5b58\u7684\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u306b\u95a2\u308f\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u3053\u306e\u8907\u5408\u4f53\u306f\u30b0\u30ea\u30aa\u30fc\u30de\u5f62\u6210\u3092\u6291\u3048\u308b\u52b9\u679c\u304c\u3042\u308b\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc2a (TOP2A) \u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u59a8\u3052\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<\/li>\n<li>\u672c\u7814\u7a76\u306b\u4f7f\u7528\u3055\u308c\u305fAGO2 wt\u306a\u3089\u3073\u306bPAZ\u30c9\u30e1\u30a4\u30f3\u5909\u7570\u4f53\u767a\u73fe\u30d9\u30af\u30bf\u30fc(RDB15797-15799)\u3001TOP2A-3&#8217;UTR\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30a2\u30c3\u30bb\u30a4\u7528\u30d9\u30af\u30bf\u30fc (RDB15800)\u3001TOP2A\u767a\u73fe\u30d9\u30af\u30bf\u30fc (RDB15801) \u3092\u5f53\u5ba4\u306b\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nDeguchi, S. et al. Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640, 2017. PMID: 28368417.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15797\">p3xFLAG-Myc-CMV-AGO2-WT (cat# RDB15797)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15798\">p3xFLAG-Myc-CMV-AGO2-PAZ-mut (cat# RDB15798)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15799\">p3xFLAG-Myc-CMV-AGO2-PAZ-del (cat# RDB15799)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15800\">pmirGLO-TOP2A-3&#8242;-UTR (cat# RDB15800)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15801\">pcDNA-DEST47-TOP2A (cat# RDB15801)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0110j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Lys48\u306b\u30ea\u30f3\u30af\u3057\u305f\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u9396\u3092\u5207\u65ad\u3059\u308b\u8131\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u9175\u7d20&#8221;USP-25&#8243;\u306e\u57fa\u8cea\u7279\u7570\u6027\u306f\u4e8c\u9023\u30e6\u30d3\u30ad\u30c1\u30f3\u7d50\u5408\u30e2\u30c1\u30fc\u30d5(UIM)\u9818\u57df\u304c\u6c7a\u3081\u3066\u3044\u308b (2020\/11\/10 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30e6\u30d3\u30ad\u30c1\u30f3\u306f\u3001\u4ed6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4fee\u98fe\u3057\u3066\u30d7\u30ed\u30c6\u30aa\u30bd\u30fc\u30e0\u3067\u306e\u5206\u89e3\u3092\u4fc3\u3059\u4ed6\u3001\u69d8\u3005\u306a\u5f79\u5272\u3092\u62c5\u3044\u307e\u3059\u3002\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u9396\u306b\u306f\u7d50\u5408\u306b\u4f7f\u308f\u308c\u308b\u30a2\u30df\u30ce\u9178\u306e\u4f4d\u7f6e\u306e\u9055\u3044\u304b\u3089\u3001Lys48\u30ea\u30f3\u30af\u3068Lys63\u30ea\u30f3\u30af\u306e2\u7a2e\u985e\u304c\u3042\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u9396\u306e\u5206\u89e3\u53cd\u5fdc\u306f\u3001Lys63\u30ea\u30f3\u30af\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u9396\u306b\u6bd4\u3079\u3001Lys 48\u30ea\u30f3\u30af\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u9396\u306e\u5206\u89e3\u6a5f\u5e8f\u306b\u3064\u3044\u3066\u306f\u7814\u7a76\u304c\u9032\u3093\u3067\u3044\u307e\u305b\u3093\u3002\u7b46\u8005\u3089\u306f\u3001\u8131\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u9175\u7d20&#8221;USP-25&#8243;\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u7d50\u5408\u90e8\u4f4d\u304c\u4e8c\u3064\u4e26\u3093\u3060\u4e8c\u9023UIM\u9818\u57df\u306e\u5909\u7570\u4f53\u3092\u4f5c\u6210\u3057\u3001\u7d50\u5408\u304c\u9175\u7d20\u6d3b\u6027\u306b\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u305f\u3046\u3048\u3067\u3001\u4e8c\u9023UIM\u9818\u57df\u304cLys 48\u30ea\u30f3\u30af\u30dd\u30ea\u30e6\u30d3\u30ad\u30c1\u30f3\u9396\u306b\u5bfe\u3059\u308b\u57fa\u8cea\u7279\u7570\u6027\u3092\u898f\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002USP-25\u306ewt\u306a\u3089\u3073\u306bmutant\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306f\u3001\u7406\u7814BRC\u3088\u308a\u63d0\u4f9b\u3057\u305f\u30d2\u30c8USP-25 cDNA \u30af\u30ed\u30fc\u30f3\u3092\u3082\u3068\u306b\u4f5c\u88fd\u3055\u308c\u307e\u3057\u305f\u3002\u7406\u7814BRC\u306b\u306f\u3001&#8221;\u30b2\u30ce\u30e0\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d7\u30ed\u30b8\u30a7\u30af\u30c8&#8221;\u306e\u30d2\u30c8cDNA\u30af\u30ed\u30fc\u30f3\u304c\u5bc4\u8a17\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u306e\u3046\u3061\u306e\u3072\u3068\u3064\u3064\u3067\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\n\tKawaguchi, K. et al. Tandem UIMs confer Lys48 ubiquitin chain substrate preference to deubiquitinase USP25. Sci. Rep. 7: 45037, 2017. PMID 28327663.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGY067544\">IRAK168O08 (cat# HGY067544)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0109j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u72d9\u3044\u901a\u308a\u306e\u5de8\u5927\u30b2\u30ce\u30e0\u9818\u57df\u3092\u6b20\u640d\u3055\u305b\u308bCRISPR-Cas9\u3092\u5229\u7528\u3057\u305f\u624b\u6cd5\u306e\u958b\u767a (2020\/11\/10 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>CRISPR-Cas9\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3067\u306f\u3001\u72d9\u3063\u305f\u30b2\u30ce\u30e0\u9818\u57df\u3001\u4f8b\u3048\u3070\u30a8\u30af\u30bd\u30f3\u306a\u3069\u3092\u6b20\u640d\u3055\u305b\u305f\u30de\u30a6\u30b9\u3092\u6a39\u7acb\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30e1\u30ac\u30d9\u30fc\u30b9\u3092\u8d85\u3048\u304b\u3064\u72d9\u3044\u901a\u308a\u306e\u30d6\u30ec\u30fc\u30af\u30dd\u30a4\u30f3\u30c8\u3092\u6301\u3064\u9818\u57df\u306e\u6b20\u640d\u3055\u305b\u308b\u52b9\u7387\u306f\u307e\u3060\u9ad8\u304f\u3042\u308a\u307e\u305b\u3093<\/li>\n<li>\u6570\u30e1\u30ac\u30d9\u30fc\u30b9\u5358\u4f4d\u306e\u9818\u57df\u6b20\u640d\u304c\u95a2\u4e0e\u3059\u308b\u30d2\u30c8\u75be\u60a3\u3092\u6a21\u5023\u3057\u305f\u30e2\u30c7\u30eb\u30de\u30a6\u30b9\u306e\u6a39\u7acb\u306b\u306f\u3053\u306e\u96e3\u984c\u3092\u514b\u670d\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002\u7b46\u8005\u3089\u306f\u3001\u30de\u30a6\u30b9Sox9\u907a\u4f1d\u5b50\u4e0a\u6d41\u306b\u30d6\u30ec\u30fc\u30af\u30dd\u30a4\u30f3\u30c8\u3092\u8a2d\u8a08\u3057\u305f\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u30de\u30a6\u30b9\u53d7\u7cbe\u5375\u306b\u5bfe\u3059\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u3088\u308a\u3001\u72d9\u3044\u901a\u308a\u306e\u30d6\u30ec\u30fc\u30af\u30dd\u30a4\u30f3\u30c8\u3092\u6301\u3064\u7d04\uff15\u30e1\u30ac\u30d9\u30fc\u30b9\u306e\u6b20\u640d\u306e\u4f5c\u6210\u3057\u305f\u4f8b\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002\u30d6\u30ec\u30fc\u30af\u30dd\u30a4\u30f3\u30c8\u306e\u78ba\u8a8d\u306b\u306f\u3001\u30de\u30a6\u30b9Sox9\u4e0a\u6d41\u914d\u5217\u306b\u4f4d\u7f6e\u3059\u308b&#8221;B6N mouse BAC clone&#8221;\u3092\u7528\u3044\u305fDNA FISH\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\n\tKato, T. et al. Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR\/Cas9 system. Sci. Rep. 7 (1): 59, 2017. PMID: 28246396.<\/li>\n<li>\u5229\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0031j\">B6N mouse BAC clones<\/a><br \/>\nB6Ng01-332K06, B6Ng01-295D01\u3001 B6Ng01-223B06, B6Ng01-111E12, B6Ng01-299I16, B6Ng01-234K06<\/li>\n<\/ul>\n<p><a name=\"EXR0108j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> Notch\u30b7\u30b0\u30ca\u30eb\u3067\u5236\u5fa1\u3055\u308c\u3066\u3044\u308b\u9577\u9396\u975e\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0RNA&#8221;TUG1&#8243;\u306f\u8133\u3053\u3046\u816b(\u30b0\u30ea\u30aa\u30fc\u30de)\u6cbb\u7642\u306e\u6a19\u7684\u3068\u306a\u308b (2020\/11\/10 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>Notch\u30b7\u30b0\u30ca\u30eb\u306f\u3001\u30b0\u30ea\u30aa\u30fc\u30de\u306e\u304c\u3093\u5e79\u7d30\u80de (GSC) \u306e\u81ea\u5df1\u8907\u88fd\u3092\u4fc3\u9032\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u81ea\u5df1\u8907\u88fd\u3092\u62c5\u3046SOX2\u3001MYC\u3001NESTIN\u3089\u306b\u3069\u306e\u3088\u3046\u306b\u95a2\u4e0e\u3059\u308b\u304b\u306f\u672a\u89e3\u660e\u3067\u3059\u3002\u7b46\u8005\u3089\u306f\u3001\u30b2\u30ce\u30e0\u60c5\u5831\u89e3\u6790\u3068lncRNA\u767a\u73fe\u91cf\u89e3\u6790\u306e\u7d50\u679c\u3001Notch\u30b7\u30b0\u30ca\u30eb\u4f9d\u5b58\u7684\u306b\u767a\u73fe\u8a98\u5c0e\u3055\u308c\u308b\u7d047kb\u306e\u9577\u9396\u975e\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0RNA &#8220;TUG1&#8243;\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u3055\u3089\u306b\u3001TUG1\u304cmiR-145\u3068\u7d50\u5408\u3057\u30a2\u30f3\u30bf\u30b4\u30cb\u30b9\u30c8\u3068\u3057\u3066\u50cd\u304f\u3053\u3068\u3067SOX2\u3084MYC\u306e\u767a\u73fe\u91cf\u3092\u4e0a\u6607\u3055\u305b\u308b\u3053\u3068\u3001\u30d2\u30b9\u30c8\u30f3H3K27\u306e\u30e1\u30c1\u30eb\u5316\u3092\u4fc3\u9032\u3059\u308b\u3053\u3068\u3067\u4e0b\u6d41\u306eBDNF\u306a\u3069\u306e\u795e\u7d4c\u6210\u9577\u56e0\u5b50\u306e\u767a\u73fe\u3092\u6291\u5236\u3057\u3001\u5e79\u7d30\u80de\u7dad\u6301\u306b\u95a2\u308f\u3063\u3066\u3044\u308b\u3053\u3068\u3001TUG1\u306e\u767a\u73fe\u6291\u5236\u306b\u3088\u308a\u751f\u4f53\u5185\u3067\u30b0\u30ea\u30aa\u30fc\u30de\u5f62\u6210\u304c\u6291\u3048\u3089\u308c\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001TUG1\u306e\u30a8\u30af\u30bd\u30f3\u6bce\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u3001\u30a8\u30af\u30bd\u30f3\uff11\u306b\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u304c\u3042\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u7814\u7a76\u306b\u4f7f\u7528\u3055\u308c\u305fTUG1\u907a\u4f1d\u5b50\u306e\uff13\u30a8\u30af\u30bd\u30f3\u5404\u3005\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306f\u3001\u7406\u7814BRC\u304b\u3089\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKatsushima, K., Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat. Commun. 7: 13616 (2016). PMID 27922002.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15794\">pTnT-TUG1 exon1 (RDB15794)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15795\">pTnT-TUG1 exon2 (RDB15795)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15796\">pTnT-TUG1 exon3 (RDB15796)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0091j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u65b0\u898f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaAchilles (2020\/3\/24 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u305f\u30e9\u30a4\u30d6\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u306f\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6210\u719f\u6642\u9593 (\u86cd\u5149\u3092\u767a\u3059\u308b\u307e\u3067\u306e\u6642\u9593)\u304c\u77ed\u3044\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u3053\u306e\u5ea6\u3001\u7406\u7814CBS\u7d30\u80de\u6a5f\u80fd\u63a2\u7d22\u6280\u8853\u7814\u7a76\u30c1\u30fc\u30e0\u306e\u65b0\u91ce \u7950\u4ecb\u5148\u751f\u3001\u5bae\u8107 \u6566\u53f2\u5148\u751f\u306f\u3001Venus\u3092\u6539\u826f\u3057\u305f\u3001\u3088\u308a\u6210\u719f\u6642\u9593\u306e\u77ed\u3044Achilles\u3092\u958b\u767a\u3055\u308c\u307e\u3057\u305f\u3002\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u4eac\u90fd\u5927\u5b66\u306e\u5f71\u5c71\u9f8d\u4e00\u90ce\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u5206\u7bc0\u6642\u8a08\u907a\u4f1d\u5b50Hes7\u3068Venus\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u3066Hes7\u306e\u52d5\u614b\u306e\u89b3\u5bdf\u3092\u8a66\u307f\u307e\u3057\u305f\u304c\u3001Hes7\u306e\u5206\u89e3\u901f\u5ea6\u304c\u901f\u304f\u3001\u86cd\u5149\u89b3\u5bdf\u304c\u56f0\u96e3\u3067\u3057\u305f\u3002\u305d\u3053\u3067\u3001Achilles\u3068Hes7\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u3066\u3001\u540c\u5b9f\u9a13\u3092\u884c\u3046\u3053\u3068\u3067Hes7\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u52d5\u614b\u89e3\u6790\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u3059\uff08Yoshioka-Kobayashi, K. et al. Nature, 2020\uff09\u3002<\/li>\n<li>\u5206\u7bc0\u6642\u8a08\u907a\u4f1d\u5b50Hes7\u306e\u5468\u671f\u7684\u306a\u8ee2\u5199\u5909\u52d5\u306f\u3001\u57f9\u990a\u7d30\u80de\u3067\u518d\u73fe\u3067\u304d\u307e\u3059\u3002Harvard Medical School \u306eDr. Olivier Pourquie\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u30b7\u30b0\u30ca\u30eb\u3092\u4ed8\u4e0e\u3057\u3066\u4e0d\u5b89\u5b9a\u5316\u3055\u305b\u305fAchilles\u3092Hes7\u907a\u4f1d\u5b50\u306e3&#8217;\u672b\u7aef\u5074\u306b\u633f\u5165\u3057\u305f\u7d30\u80de\u682a\u3092\u6a39\u7acb\u3057\u3001\u3053\u306e\u7d30\u80de\u3067Hes7-Achilles\u907a\u4f1d\u5b50\u306e\u5468\u671f\u7684\u306a\u8ee2\u5199\u5909\u52d5\u3092\u5358\u4e00\u7d30\u80de\u30ec\u30d9\u30eb\u3067\u691c\u51fa\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u3059\u3002\uff08Diaz-Cuadros, M. et al. Nature, 2020\uff09\u3002<\/li>\n<li>\u8ad6\u6587\u3001\u95a2\u9023\u8a18\u4e8b<br \/>\nYoshioka-Kobayashi, K. et al. Coupling delay controls synchronized oscillation in the segmentation clock. Nature, 2020. PMID: 31915376<br \/>\nDiaz-Cuadros, M. et al. In vitro characterization of the human segmentation clock. Nature, 2020. PMID: 31915384<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15982\">Achilles_pRSETB (cat# RDB15982)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0107j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30b0\u30eb\u30b3\u30b3\u30eb\u30c1\u30b3\u30a4\u30c9\u306f\u3001T\u7d30\u80de\u4e0a\u306ePD-1\u3092\u30a2\u30c3\u30d7\u30ec\u30ae\u30e5\u30ec\u30fc\u30c8\u3059\u308b(2020\/2\/18 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>T\u7d30\u80de\u306f\u3001\u8a98\u5c0e\u7684\u306b\u767a\u73fe\u3059\u308bPD-1\u3084\u514d\u75ab\u6291\u5236\u5264\u306e\u30b0\u30eb\u30b3\u30b3\u30eb\u30c1\u30b3\u30a4\u30c9(GC)\u306b\u3088\u308a\u6d3b\u6027\u5316\u304c\u6291\u5236\u3055\u308c\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059<\/li>\n<li>\u524d\u7530\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u5404\u7a2e\u514d\u75ab\u6291\u5236\u5264\u306ePD-1\u767a\u73fe\u3078\u306e\u5f71\u97ff\u3092\u8abf\u3079\u308b\u7814\u7a76\u3067\u3001GC\u304cPD-1\u306e\u767a\u73fe\u3092\u5897\u5f37\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001PD-1\u306e\u767a\u73fe\u5897\u5f37\u306f\u3001PD-1\u907a\u4f1d\u5b50\u4e0a\u6d41\u306e\u8ee2\u5199\u8abf\u7bc0\u9818\u57df\u306b\u3042\u308bGC\u5fdc\u7b54\u914d\u5217\u306b\u4f9d\u5b58\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u3053\u306e\u7814\u7a76\u3067\u7528\u3044\u3089\u308c\u305fPD-1\u306e\u8ee2\u5199\u8abf\u7bc0\u9818\u57df\u3092\u542b\u3080\u30b2\u30ce\u30e0DNA\u306f\u3001\u5f53\u5ba4\u306eB6N BAC\u30af\u30ed\u30fc\u30f3(B6Ng01-240G08)\u304b\u3089\u3001PCR\u306b\u3088\u3063\u3066\u5897\u5e45\u3055\u308c\u305f\u3082\u306e\u3067\u3059\u3002<\/li>\n<li>\u8ad6\u6587\u3001\u95a2\u9023\u8a18\u4e8b<br \/>\nMaeda, N. et al. Glucocorticoids potentiate the inhibitory capacity of programmed cell death 1 by up-regulating its expression on T cells. J. Biol. Chem., 294 (52): 19896-19906, 2019. PMID: 31723031<\/li>\n<li>\u30ea\u30bd\u30fc\u30b9\u60c5\u5831<br \/>\n<a href=\"https:\/\/dna.brc.riken.jp\/ja\/clonesetja\">\u30af\u30ed\u30fc\u30f3\u30bb\u30c3\u30c8&#038;\u30b2\u30ce\u30e0DNA -Genomic clone-<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0106j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> BAC\u30af\u30ed\u30fc\u30f3 (2020\/2\/18 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> \u7406\u7814BRC DNA Bank\u306f\u3001\u30de\u30a6\u30b9 (C57BL\/6N\u53ca\u3073MSM\/Ms\u7cfb\u7d71)\u3001\u30e9\u30c3\u30c8\uff08F344\/Stm\u53ca\u3073LE\/Stm\u7cfb\u7d71\uff09\u3001\u30cb\u30db\u30f3\u30b6\u30eb\u3001\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\uff08D. melanogaster\u306a\u30695\u7a2e\uff09\u306eBAC\uff08\u30d0\u30af\u30c6\u30ea\u30a2\u4eba\u5de5\u67d3\u8272\u4f53\uff09\u30af\u30ed\u30fc\u30f3\u3092\u6574\u5099\u3001\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u3054\u5e0c\u671b\u306eBAC\u30af\u30ed\u30fc\u30f3\u306f\u3001\u5c02\u7528\u306e\u30d6\u30e9\u30a6\u30b6\u30fc\u3092\u7528\u3044\u3066\u907a\u4f1d\u5b50\u540d\u3067\u691c\u7d22\u3067\u304d\u3001\u307e\u305f\u30b2\u30ce\u30e0\u9818\u57df\u4e0a\u306e\u30af\u30ed\u30fc\u30f3\u306e\u4f4d\u7f6e\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>BAC\u30af\u30ed\u30fc\u30f3\u306f\u3001\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u52d5\u7269\u6a39\u7acb\u7528\u306e\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3084LacZ\u306b\u3088\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u30ec\u30dd\u30fc\u30bf\u30fc\u3092\u5c0e\u5165\u3057\u305f\u52d5\u7269\u306e\u4f5c\u88fd\u306a\u3069\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>BAC\u30af\u30ed\u30fc\u30f3\u306f\u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u8abf\u7bc0\u9818\u57df\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306b\u3082\u5229\u7528\u3055\u308c\u3066\u304a\u308a\u3001\u300c\u30b0\u30eb\u30b3\u30b3\u30eb\u30c1\u30b3\u30a4\u30c9\u306f\u3001T\u7d30\u80de\u4e0a\u306ePD-1\u3092\u30a2\u30c3\u30d7\u30ec\u30ae\u30e5\u30ec\u30fc\u30c8\u3059\u308b (2020\/2\/18 N.N.)\u300d\u3067\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u30ea\u30bd\u30fc\u30b9\u60c5\u5831<br \/>\n<a href=\"https:\/\/dna.brc.riken.jp\/ja\/clonesetja\">\u30af\u30ed\u30fc\u30f3\u30bb\u30c3\u30c8&#038;\u30b2\u30ce\u30e0DNA -Genomic clone-<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0102j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Atg2\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b4\u30bd\u30fc\u30e0\u819c\u306e\u5f62\u6210\u306b\u5fc5\u8981\u306a\u8102\u8cea\u5206\u5b50\u306e\u8f38\u9001\u306b\u76f4\u63a5\u95a2\u4e0e\u3059\u308b\u3002(2019\/12\/16 N.K.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306f\u3001\u7d30\u80de\u5185\u3067\u4e0d\u8981\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u89e3\u30fb\u518d\u5229\u7528\u3059\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u306e1\u3064\u3067\u3059\u3002\u4e0d\u8981\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u307e\u305a\u30ea\u30f3\u8102\u8cea\u819c\u69cb\u9020\u306e\u30aa\u30fc\u30c8\u30d5\u30a1\u30b4\u30bd\u30fc\u30e0\u5185\u306b\u9694\u96e2\u3055\u308c\u307e\u3059\u3002\u30aa\u30fc\u30c8\u30d5\u30a1\u30b4\u30bd\u30fc\u30e0\u751f\u5408\u6210\u306b\u306f\u6570\u7a2e\u306eAtg\u30bf\u30f3\u30d1\u30af\u8cea\u7fa4\u304c\u95a2\u308f\u3063\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u672c\u7814\u7a76\u306b\u304a\u3044\u3066\u3001\u7b46\u8005\u3089\u306f\u3001Atg2\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u3001ER\u3068\u30aa\u30fc\u30c8\u30d5\u30a1\u30b4\u30bd\u30fc\u30e0\u3092\u3064\u306a\u3050\u30c6\u30b6\u30ea\u30f3\u30b0\u6d3b\u6027\u3092\u6301\u3061\u3001\u304b\u3064ER\u819c\u304b\u3089\u30aa\u30fc\u30c8\u30d5\u30a1\u30b4\u30bd\u30fc\u30e0\u819c\u3078\u306e\u30ea\u30f3\u8102\u8cea\u5206\u5b50\u306e\u8f38\u9001\u306b\u76f4\u63a5\u7684\u306b\u304b\u304b\u308f\u308b\u3053\u3068\u3092\u3001\uff38\u7dda\u7d50\u6676\u69cb\u9020\u89e3\u6790\u3001\u304a\u3088\u3073FRET\u3092\u5fdc\u7528\u3057\u3066\u958b\u767a\u3057\u305f\u65b0\u898f\u306e\u30ea\u30d4\u30c9\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u30a2\u30c3\u30bb\u30a4\u6cd5\u3092\u7528\u3044\u3066\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u306b\u306f\u3001\u51fa\u82bd\u9175\u6bcd\u3068\u5206\u88c2\u9175\u6bcd\u7531\u6765\u306eAtg2\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7528\u3044\u3089\u308c\u3066\u304a\u308a\u3001\u5206\u88c2\u9175\u6bcdAtg2\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092\u69cb\u7bc9\u3059\u308b\u969b\u306b\u5f53\u5ba4\u304c\u63d0\u4f9b\u3057\u305fSpFFH31A11 (SPW052411)\u304c\u7528\u3044\u3089\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nOsawa, T. et al. Atg2 mediates direct lipid transfer between membranes for autophagosome formation. PMID 30911189.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/SPW052411\">SpFFH31A11 (SPW052411)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0101j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>\u52b9\u7387\u7684\u306aPCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u305f\u3081\u306e\u65b0\u898f\u958b\u767a\u30d9\u30af\u30bf\u30fc (2019\/10\/11 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> PCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u65b0\u898f\u30d9\u30af\u30bf\u30fc\u304c\u3001\u4eac\u90fd\u7523\u696d\u5927\u5b66\u751f\u547d\u79d1\u5b66\u90e8\u306e\u672c\u6a4b \u5065 \u5148\u751f\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u3001\u672c\u5e744\u6708\u306b\u8ad6\u6587\u3068\u3057\u3066\u767a\u8868\u3055\u308c\u307e\u3057\u305f(\u4e0b\u8a18\u6587\u732e\u7b49\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044)\u3002TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u7528\u3001\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u7528\u3001\u4e26\u3073\u306b\u517c\u7528\u306e3\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9\n<ul style=\"line-height: 1.5em;\">\n<li>TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc; <a href=\"https:\/\/brc.riken.jp\/dna\/RDB17479\">pCRT (cat# RDB17479)<\/a><br \/>\n\u5236\u9650\u9175\u7d20Xcm I\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u51e6\u7406\u3059\u308b\u3053\u3068\u3067TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc; <a href=\"https:\/\/brc.riken.jp\/dna\/RDB17481\">pCRZero (cat# RDB17481)<\/a><br \/>\n\u5236\u9650\u9175\u7d20EcoR V\u3067\u672c\u30d9\u30af\u30bf\u30fc\u3092\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>TA-\u3001\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u517c\u7528\u30d9\u30af\u30bf\u30fc; <a href=\"https:\/\/brc.riken.jp\/dna\/RDB17480\">pCRZeroT (cat# RDB17480)<\/a><br \/>\n\u5236\u9650\u9175\u7d20Xcm I\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u3001\u5236\u9650\u9175\u7d20Sma I\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u3067\u5e73\u6ed1\u672b\u7aef\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306b\u305d\u308c\u305e\u308c\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<p><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/exr0101map.png\" alt=\"\" width=\"100%\" \/><\/p>\n<li>\u8ad6\u6587<br \/>\nMotohashi, K. A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417, 2019. PMID: 31015513.<\/li>\n<li>\u53c2\u8003web\u30b5\u30a4\u30c8<br \/>\n<a href=\"https:\/\/www.kyoto-su.ac.jp\/news\/20190424_400a_news.html\">\u751f\u547d\u79d1\u5b66\u90e8 \u672c\u6a4b \u5065 \u6559\u6388\u304cPCR\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u305f\u3081\u306e\u65b0\u3057\u3044\u30d9\u30af\u30bf\u30fc\u3092\u958b\u767a\u3057\u307e\u3057\u305f (\u4eac\u90fd\u7523\u696d\u5927\u5b662019\/4\/24)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0100j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u7d44\u7e54\u3092\u69cb\u6210\u3059\u308b\u7d30\u80de\u306e\u5f62\u614b\u3084\u914d\u5217\u3092\u8abf\u7bc0\u3059\u308b\u5236\u5fa1\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u89e3\u660e (2019\/9\/6 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7d44\u7e54\u3092\u69cb\u6210\u3059\u308b\u7d30\u80de\u306e\u5f62\u614b\u3068\u914d\u5411\u306f\u7d30\u80de\u9aa8\u683c\u306e\u52d5\u614b\u3084\u7d30\u80de\u6975\u6027\u306b\u3088\u3063\u3066\u6c7a\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7d30\u80de\u9aa8\u683c\u306e\u52d5\u614b\u5909\u5316\u306f\u3001\u60c5\u5831\u4f1d\u9054\u7269\u8cea\u306e\u3084\u308a\u3068\u308a\u306b\u3088\u3063\u3066\u8a98\u5c0e\u3055\u308c\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u3069\u306e\u69d8\u306b\u3057\u3066\u7d30\u80de\u9aa8\u683c\u306e\u52d5\u614b\u306b\u53cd\u6620\u3055\u308c\u308b\u304b\u3001\u305d\u306e\u5236\u5fa1\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u672a\u89e3\u660e\u3067\u3057\u305f\u3002<\/li>\n<li>\u30b2\u30ce\u30e0\u7de8\u96c6\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u304c\u81f4\u6b7b\u3001\u3042\u308b\u3044\u306f\u691c\u51fa\u304c\u5bb9\u6613\u306a\u8868\u73fe\u578b\u3092\u793a\u3055\u306a\u3044\u3088\u3046\u306a\u5834\u5408\u3001\u6a19\u7684\u5916\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3092\u6291\u3048\u308b\u3053\u3068\u3067\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3068\u81f4\u6b7b\u907a\u4f1d\u5b50\u540c\u5b9a\u306b\u5fc5\u8981\u306a\u52b4\u529b\u3092\u5c11\u3057\u3067\u3082\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30e9\u30f3\u30c0\u30e0\u306b\u30b2\u30ce\u30e0\u306b\u633f\u5165\u3055\u308c\u308b\u30d9\u30af\u30bf\u30fc\u6570\u304c\u591a\u3044\u3068\u30b2\u30ce\u30e0\u3078\u306e\u904e\u5270\u306aCas9\u9175\u7d20\u306e\u30a2\u30bf\u30c3\u30af\u304c\u8d77\u304d\u3001Off-target\u306e\u539f\u56e0\u3068\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u718a\u672c\u5927\u5b66\u5927\u5b66\u9662\u751f\u547d\u79d1\u5b66\u7814\u7a76\u90e8\u306e\u83ca\u6c60 \u6d69\u4e8c\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001Wnt5a\u306e\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u304c\u7d30\u80de\u9aa8\u683c\u3092\u69cb\u6210\u3059\u308b\u5fae\u5c0f\u7ba1\u306e\u52d5\u614b\u5236\u5fa1\u306b\u95a2\u308f\u308b\u3053\u3068\u306b\u7740\u76ee\u3057\u3001\u9593\u3092\u3068\u308a\u3082\u3064\u5206\u5b50\u3092\u63a2\u7d22\u3059\u308b\u305f\u3081\u3001siRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306b\u3088\u308b\u8868\u73fe\u578b\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3044\u3001Map7\/7D1\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002\u7d9a\u3044\u3066\u3001\u57f9\u990a\u7d30\u80de\u3084\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u500b\u4f53\u3092\u7528\u3044\u305fMap7\/7D1\u306e\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u5b9f\u9a13\u306a\u3069\u3092\u7d4c\u3066\u3001Map7\/7D1\u304cWnt5a\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u306b\u304a\u3051\u308b\u4f1d\u9054\u7269\u8cea\u306e\u4e00\u3064\u3067\u3042\u308bDvl\u3068\u7d50\u5408\u3059\u308b\u3053\u3068\u3001Map7\/7D1\u304cDvl\u306e\u5c40\u5728\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u3001Wnt5a\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u3068\u5fae\u5c0f\u7ba1\u306e\u52d5\u614b\u5909\u5316\u3092\u7d50\u3073\u3064\u3051\u308b\u5236\u5fa1\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u672c\u7814\u7a76\u306b\u306f\u5f53\u5ba4\u3088\u308a\u63d0\u4f9b\u3057\u305fMAP7\u3092\u30b3\u30fc\u30c9\u3059\u308b\u30af\u30ed\u30fc\u30f3(IRAK049L15)\u304c\u7528\u3044\u3089\u308c\u3066\u304a\u308a\u3001\u86cd\u5149\u30e9\u30a4\u30d6\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306e\u305f\u3081\u306b\u4f5c\u88fd\u3057\u305fpEGFP-N3-hMap7\u306b\u3088\u308aMap7\/7D1\u306e\u7d30\u80de\u5185\u5c40\u5728\u3092\u89e3\u6790\u3057\u3066\u3044\u307e\u3059\u3002pEGFP-N3-hMap7\u306e\u4ed6\u3001\u8a083\u7a2e\u985e\u306e\u30af\u30ed\u30fc\u30f3\u306f\u3001\u83ca\u6c60\u5148\u751f\u306e\u3054\u539a\u610f\u306b\u3088\u308a\u5f53\u5ba4\u306b\u5bc4\u8a17\u3057\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\u7686\u69d8\u306e\u7a4d\u6975\u7684\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059<\/li>\n<li>\u8ad6\u6587<br \/>\nKikuchi, K. et al. Map7\/7D1 and Dvl form a feedback loop that facilitates microtubule remodeling and Wnt5a signaling.  EMBO Rep. 19 (7): e45471, 2018. PMID: 29880710.<\/li>\n<li>\u30d7\u30ec\u30b9<br \/>\n<a href=\"https:\/\/www.kumamoto-u.ac.jp\/daigakujouhou\/kouhou\/pressrelease\/2018-file\/release180607.pdf\">\u7d44\u7e54\u306e\u5f62\u6210\u306b\u95a2\u308f\u308b\u3001\u7d30\u80de\u306e\u300c\u304b\u305f\u3061\u300d\u3084\u300c\u306a\u3089\u3073\u300d\u3092\u8abf\u7bc0\u3059\u308b\u65b0\u3057\u3044\u4ed5\u7d44\u307f\u306e\u89e3\u660e (\u718a\u672c\u5927\u5b662018\/6\/7)<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9\u3001\u63d0\u4f9b\u3057\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16943\">pEGFP-N3-hMap7 (cat# RDB16943)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16944\">pcDNA3.1-V5His6-hMap7 (cat# RDB16944)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16945\">pEGFP-N3-mMap7D1 (cat# RDB16945)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGX019879\">IRAK049L15 (cat# HGX019879)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0099j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u9ad8\u52b9\u7387\u30b2\u30ce\u30e0\u7de8\u96c6\u7528\u30d9\u30af\u30bf\u30fc pGedit (2019\/7\/26 H.S.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7523\u696d\u6280\u8853\u7dcf\u5408\u7814\u7a76\u6240 \u9577\u5d0e \u6643\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u305f\u3001\u9ad8\u52b9\u7387\u30b2\u30ce\u30e0\u7de8\u96c6\u7528\u30d9\u30af\u30bf\u30fc pGedit \u304c\u5230\u7740\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u30b2\u30ce\u30e0\u7de8\u96c6\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u304c\u81f4\u6b7b\u3001\u3042\u308b\u3044\u306f\u691c\u51fa\u304c\u5bb9\u6613\u306a\u8868\u73fe\u578b\u3092\u793a\u3055\u306a\u3044\u3088\u3046\u306a\u5834\u5408\u3001\u6a19\u7684\u5916\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3092\u6291\u3048\u308b\u3053\u3068\u3067\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3068\u81f4\u6b7b\u907a\u4f1d\u5b50\u540c\u5b9a\u306b\u5fc5\u8981\u306a\u52b4\u529b\u3092\u5c11\u3057\u3067\u3082\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30e9\u30f3\u30c0\u30e0\u306b\u30b2\u30ce\u30e0\u306b\u633f\u5165\u3055\u308c\u308b\u30d9\u30af\u30bf\u30fc\u6570\u304c\u591a\u3044\u3068\u30b2\u30ce\u30e0\u3078\u306e\u904e\u5270\u306aCas9\u9175\u7d20\u306e\u30a2\u30bf\u30c3\u30af\u304c\u8d77\u304d\u3001Off-target\u306e\u539f\u56e0\u3068\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u9577\u5d0e\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304c\u958b\u767a\u3057\u305fpGedit (cat# RDB16763)\u306fblasticidin S \u8010\u6027\u907a\u4f1d\u5b50 (Bsr) \u3092\u6301\u3064Cas9\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3067\u3059\u3002\u3088\u308a\u77ed\u6642\u9593\u3067\u7d30\u80de\u3092\u9078\u629e\u3067\u304d\u308bblasticidin S \u3092\u4f7f\u3044\u3001blasticidin S\u306e\u57f9\u5730\u6dfb\u52a0\u309224\uff5e48\u6642\u9593\u306e\u307f\u306b\u9650\u5b9a\u3059\u308b\u3053\u3068\u3067\u30d9\u30af\u30bf\u30fc\u306e\u30b2\u30ce\u30e0\u3078\u306e\u30a4\u30f3\u30c6\u30b0\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u6291\u3048\u3001\u3055\u3089\u306bEGFP\u3092Bsr\u3068\u878d\u5408\u3057\u3066\u767a\u73fe\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30d9\u30af\u30bf\u30fc\u306e\u6b8b\u5b58\u3092\u30e2\u30cb\u30bf\u30fc\u3067\u304d\u308b\u3088\u3046\u306a\u5de5\u592b\u3092\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001sgRNA\u30b9\u30ab\u30d5\u30a9\u30fc\u30eb\u30c9\u5185\u306b\u3042\u308bRNA polymerase III terminal signal \uff08TTTT\uff09\u3092\u53d6\u308a\u9664\u304f\u3053\u3068\u3067sgRNA\u91cf\u3092\u5897\u52a0\u3055\u305b\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306e\u5897\u52a0\u3092\u671f\u5f85\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u3053\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u6709\u7528\u6027\u3092\u307f\u308b\u305f\u3081\u3001\u30d2\u30c8\u9aa8\u8089\u816b\u4e0a\u76ae\u7d30\u80deU2OS\u3092\u7528\u3044\u30662\u7a2e\u985e\u306e\u30d2\u30c8\u7d30\u80de\u8cea\u30a2\u30af\u30c1\u30f3\u907a\u4f1d\u5b50\u3001ACTB\u3068ACTG1\u306b\u5bfe\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u3092pGedit\u306b\u633f\u5165\u3057\u3001\u3053\u306e\uff12\u3064\u306e\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7d30\u80de\u682a\u3092\u53d6\u5f97\u3059\u308b\u5b9f\u9a13\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u305d\u308c\u305d\u308c\u306e\u907a\u4f1d\u5b50\u306b\u3064\u3044\u3066\u7d0420\u306e\u5019\u88dc\u7d30\u80de\u304b\u3089\u5c11\u306a\u304f\u3068\u30825\u500b\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7d30\u80de\u682a\u3092\u5f97\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nNagasaki, A. et al. A genome editing vector that enable easy selection and identification of knockout cells. Plasmid, 98: 37-44, 2018. PMID : 30196057.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16763\">pGedit (cat# RDB16763)<\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB16763.png\" width=\"100%\" \/><\/li>\n<\/ul>\n<p><a name=\"EXR0098j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8d77\u3053\u3063\u305f\u7d30\u80de\u3092\u52b9\u7387\u826f\u304f\u9078\u629e\u3059\u308b\u305f\u3081\u306e\u30d7\u30e9\u30b9\u30df\u30c9 pCAG-NexxoR (2019\/6\/21 H.S.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u56fd\u7acb\u7cbe\u795e\u30fb\u795e\u7d4c\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc \u7167\u5149\u5b9f\u52a0\u5148\u751f\u3001\u6a4b\u6238\u548c\u592b\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u305f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8a2d\u8a08\u901a\u308a\u306b\u8d77\u304d\u305f\u7d30\u80de\u3092\u85ac\u5264\u8010\u6027\u306b\u3088\u308a\u52b9\u7387\u7684\u306b\u6fc3\u7e2e\u3067\u304d\u308b\u30d7\u30e9\u30b9\u30df\u30c9 pCAG-NexxoR \u304c\u5230\u7740\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u307e\u305a\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u3092\u542b\u3080\u30b2\u30ce\u30e0\u65ad\u7247\u3092\u633f\u5165\u3057\u305fpCAG-NexxoR \u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u3068\u3057\u3066\u4f5c\u88fd\u3057\u307e\u3059\u3002\u6b21\u306b\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u5fc5\u8981\u306a\u9175\u7d20\u304a\u3088\u3073\u30ac\u30a4\u30c9RNA\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u3068\u5171\u306b\u7d30\u80de\u306b\u5c0e\u5165\u3057\u307e\u3059\u3002\u305d\u306e\u5f8cG418\u3092\u52a0\u3048\u305f\u57f9\u5730\u3067\u8010\u6027\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u4e0a\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8d77\u304d\u305f\u7d30\u80de\u306e\u307f\u304c\u751f\u80b2\u3057\u9ad8\u52b9\u7387\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u304c\u8d77\u304d\u305f\u7d30\u80de\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n\u7167\u5149\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u308bC2C12\u57f9\u990a\u7d30\u80de\u3092\u7528\u3044\u305f\u30d1\u30a4\u30ed\u30c3\u30c8\u5b9f\u9a13\u3067\u306f\u3001\u7d0498%\u306e\u52b9\u7387\u3067\u5909\u7570\u4f53\u304c\u5f97\u3089\u308c\u3001\u5909\u7570\u4f53\u4e2d\u306e\u7d04\uff12\uff05\u304c\u30db\u30e2\u5909\u7570\u4f53\u3067\u3057\u305f\u3002\uff08\u56f3\uff11\uff09<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16803\">pCAG-NexxoR (cat# RDB16803)<\/a><br \/>\npCAG-EGxxFP (RDB13532)\u306eEGFP\u3092\u30cd\u30aa\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u306b\u7f6e\u304d\u63db\u3048\u6539\u5909\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002<\/li>\n<li>\u5b9f\u9a13\u7d50\u679c\u306e\u4e00\u4f8b<br \/>\n\u30b2\u30ce\u30e0\u7de8\u96c6\u7528\u30d7\u30e9\u30b9\u30df\u30c9pX330-\u30ac\u30a4\u30c9RNA\uff08pX330\u306b\u30ac\u30a4\u30c9RNA\u914d\u5217\u3092\u633f\u5165)\u3001pCAG-EGxxFP-target (pCAG-EGxxFP\u306btarget\u914d\u5217\u3092\u633f\u5165)\u3092C2C12\u7d30\u80de\u306bCotransfection\u3057\u300124\u6642\u9593\u5f8c\u304b\u3089G418\u6dfb\u52a0\u57f9\u5730\u3067\u57f9\u990a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3068\u304d\u3001pCAG-NexxoR-target\uff08pCAG-NexxoR\u306btarget\u914d\u5217\u3092\u633f\u5165\uff09\u3001\u3042\u308b\u3044\u306fpCAG-NeoR (G418\u8010\u6027\u907a\u4f1d\u5b50\u767a\u73fe\u30d9\u30af\u30bf\u30fc) \u3082Transfection\u3057\u307e\u3057\u305f\u3002<br \/>\n\u3053\u306e\u7d50\u679c\u304b\u3089\u3001\u85ac\u5264\u8010\u6027\u907a\u4f1d\u5b50\u306e\u5171\u767a\u73fe\u3060\u3051\u3067\u306f\u9078\u5225\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u793a\u3055\u308cNexxoR\u306e\u6709\u7528\u6027\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\uff08\u5b9f\u9a13\u7d50\u679c\u306f\u3001\u56fd\u7acb\u7cbe\u795e\u30fb\u795e\u7d4c\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u7167\u5149\u5148\u751f\u306b\u63d0\u4f9b\u3057\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\uff09<br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/RDB16803_1en.png\" width=\"100%\" \/><br \/>\n\u56f3\uff11\uff0e\u5de6\uff1a G418\u6dfb\u52a048\u6642\u9593\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u751f\u3058\u305f\u7d30\u80de\uff08GFP\u967d\u6027\uff09\u304c\u9078\u5225\u3055\u308c\u3001GFP\u9670\u6027\u7d30\u80de\u306f\u307b\u307c\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<br \/>\n\u4e2d\uff1a G418\u6dfb\u52a0\u7121\u3067\u306fGFP\u9670\u6027\u7d30\u80de\u304c\u591a\u304f\u898b\u3089\u308c\u307e\u3057\u305f\u3002<br \/>\n\u53f3\uff1a pCAG-NeoR\u306eCotransfection\u3067\u306f\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u751f\u3058\u305f\u7d30\u80de\uff08GFP\u967d\u6027\uff09\u304c\u9078\u5225\u3055\u308c\u305aGFP\u9670\u6027\u7d30\u80de\u304c\u591a\u304f\u898b\u3089\u308c\u307e\u3057\u305f\u3002\n<\/li>\n<\/ul>\n<p><a name=\"EXR0092j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/>  IL1B\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u306b\u304a\u3051\u308b\u8ee2\u5199\u30b3\u30a2\u30af\u30c1\u30d9\u30fc\u30bf\u30fcTAZ\u306e\u6a5f\u80fd (2019\/05\/13 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> \u60aa\u6027\u4e2d\u76ae\u816b\u306e\u8868\u73fe\u578b\u306bYAP\u907a\u4f1d\u5b50\u304c\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304c\u3053\u308c\u307e\u3067\u793a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001YAP\u306e\u30d1\u30e9\u30ed\u30b0\u3067\u3042\u308bTAZ\u306e\u60aa\u6027\u4e2d\u76ae\u816b\u3078\u306e\u95a2\u4e0e\u306f\u5341\u5206\u306b\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/li>\n<li>\u672c\u8ad6\u6587\u3067\u306f\u3001\u6d3b\u6027\u578bTAZ (S89A)\u306b\u3088\u308a\u3001\u4e0d\u6b7b\u5316\u4e2d\u76ae\u7d30\u80de\u304c\u5f62\u8cea\u8ee2\u63db\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308bmRNA\u767a\u73fe\u89e3\u6790\u3068\u907a\u4f1d\u5b50\u30aa\u30f3\u30c8\u30ed\u30b8\u30fc\u89e3\u6790\u306e\u7d50\u679c\u3001TAZ\u304c\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u5236\u5fa1\u306b\u95a2\u308f\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\u60aa\u6027\u8868\u73fe\u578b\u306b\u304a\u3051\u308bTAZ\u306e\u6a5f\u80fd\u306f\u3001\u6d3b\u6027\u578bTAZ\u306e\u767a\u73fe\u5f8c\u306eIL1A, IL1B, IL6\u306a\u3089\u3073\u306bIL24\u306emRNA\u767a\u73fe\u304a\u3088\u3073\u5404\u907a\u4f1d\u5b50\u306e\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u306b\u3088\u308b\u7d30\u80de\u5897\u6b96\u3078\u306e\u5f71\u97ff\u306b\u3088\u308a\u8abf\u3079\u3089\u308c\u3001IL1B\u304cTAZ\u306e\u4e0b\u6d41\u907a\u4f1d\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002<\/li>\n<li>TAZ\u306b\u3088\u308bIL1B\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u6d3b\u6027\u5316\u306e\u691c\u5b9a\u306b\u3001pKM2L-phIL1B\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30ec\u30dd\u30fc\u30bf\u30fc\u304c\u4f7f\u308f\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nMatsushita, A., Sato, T., Mukai, S., Fujishita, T., Mishiro-Sato, E., Okuda, M., Aoki, M., Hasegawa, Y., Sekido, Y.. TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation. Oncogene 38: 1966-1978 (2019). PMID 30401981.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05526\">pKM2L-phIL1B (cat# RDB05526)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0096j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> HMGA1\u306fPLAU\u3068SERPINE1\u907a\u4f1d\u5b50\u306e\u5236\u5fa1\u3092\u901a\u3058\u3066urokinase plasminogen activator\u30b7\u30b9\u30c6\u30e0\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b (2019\/04\/12 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> HMGA1\u306f\u3001\u304c\u3093\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e1\u30fc\u30b7\u30e7\u30f3\u306e\u69d8\u3005\u306a\u5074\u9762\u306e\u907a\u4f1d\u5b50\u5236\u5fa1\u306b\u95a2\u308f\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u7b46\u8005\u3089\u306f\u59cb\u3081\u306b\u3001HMGA1\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306b\u5bfe\u5fdc\u3059\u308b\u5206\u6ccc\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u7fa4\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u3046\u3061\u3001\u6700\u3082\u3088\u304f\u89e3\u6790\u3055\u308c\u305f\u304c\u3093\u306e\u6d78\u6f64\u3068\u62e1\u6563\u306b\u95a2\u308f\u308b\u7d4c\u8def\u3067\u3042\u308burokinase plasminogen activator\u30b7\u30b9\u30c6\u30e0\u306b\u5f71\u97ff\u3059\u308bPLAU\u3068SERPINE1\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u8abf\u7bc0\u9818\u57df\u306b\u5bfe\u3057\u3066\u6b63\u306e\u8abf\u7bc0\u6a5f\u80fd\u304c\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u4e21\u907a\u4f1d\u5b50\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u89e3\u6790\u306bpGL4-phPLAU (RDB07487) \u3068pGL4-phSERPINE1(PAI-1) (RDB07461) \u304c\u4f7f\u308f\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nResmini, G., Rizzo, S., Franchin, C., Zanin, R., Penzo, C., Pegoraro, S., Ciani, Y., Piazza, S., Arrigoni, G., Sgarra, R., Manfioletti, G. HMGA1 regulates the Plasminogen activation system in the secretome of breast cancer cells. Sci. Rep. 7 (1): 11768 (2017). PMID: 28924209.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07487\">pGL4-phPLAU (cat# RDB07487)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07461\">pGL4-phSERPINE1(PAI-1) (cat# RDB07461)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0090j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u7d30\u80de\u5185\u306e\u7570\u7a2e\u30aa\u30eb\u30ac\u30cd\u30e9\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u90e8\u4f4d\u3092\u53ef\u8996\u5316\u3067\u304d\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u30d7\u30ed\u30fc\u30d6(2019\/3\/29 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u5206\u65ad\u3055\u308c\u305fGFP\u306f\u3001\u81f3\u8fd1\u8ddd\u96e2\u3067\u5b58\u5728\u3059\u308b\u3068\u518d\u69cb\u6210\u3055\u308c\u3001\u518d\u3073\u86cd\u5149\u3092\u767a\u5149\u3059\u308b\u3068\u3044\u3046\u7279\u5fb4\u3092\u6709\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3084\u5c0f\u80de\u4f53\u7b49\u306e\u30aa\u30eb\u30ac\u30cd\u30e9\u3092\u305d\u308c\u305e\u308c\u30b9\u30d7\u30ea\u30c3\u30c8GFP\u3067\u30bf\u30b0\u4ed8\u3051\u3057\u3066\u304a\u304f\u3068\u3001\u30aa\u30eb\u30ac\u30cd\u30e9\u304c\u7d30\u80de\u5185\u3067\u63a5\u89e6\u3057\u3066\u3044\u308b\u5834\u6240\u3067GFP\u306e\u518d\u69cb\u6210\u304c\u8d77\u304d\u3001\u305d\u306e\u4f4d\u7f6e\u304c\u86cd\u5149\u306b\u3088\u308a\u53ef\u8996\u5316\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u539f\u7406\u3092\u5229\u7528\u3057\u305f\u30d7\u30ed\u30fc\u30d6\u30d9\u30af\u30bf\u30fc\u30b7\u30ea\u30fc\u30ba\u304c\u5c71\u5f62\u5927\u5b66\u306e\u7530\u6751 \u5eb7\u5148\u751f\u3001\u4eac\u90fd\u7523\u696d\u5927\u5b66\u306e\u9060\u85e4 \u6597\u5fd7\u4e5f\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u3001\u5f53\u5ba4\u304b\u3089\u3054\u5229\u7528\u3067\u304d\u307e\u3059\u3002<br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/grp0061_splitGFP_300.png\" alt=\"\" \/><\/li>\n<li>\u8ad6\u6587<br \/>\nKakimoto, Y et al. Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep., 8 (1): 6175, 2018. PMID : 29670150.<\/li>\n<p><a href=\"https:\/\/www.kyoto-su.ac.jp\/news\/20180419_345_release_ka01.html\">\u30aa\u30eb\u30ac\u30cd\u30e9\uff08\u7d30\u80de\u5c0f\u5668\u5b98\uff09\u9593\u76f8\u4e92\u4f5c\u7528\u306e\u53ef\u8996\u5316\u306b\u6210\u529f\uff5e\u7d30\u80de\u5185\u69cb\u9020\u306e\u3053\u308c\u307e\u3067\u306e\u6982\u5ff5\u3092\u4e00\u65b0\uff5e [\u4eac\u90fd\u7523\u696d\u5927\u5b66\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9, 2018\/4\/19] [link]<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0061j\">\u7530\u6751\u5148\u751f\u304b\u3089\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9[link]<\/a>\u306e\u30da\u30fc\u30b8\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<p><a name=\"EXR0047j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> DNA\u30df\u30b9\u30de\u30c3\u30c1\u4fee\u5fa9\u306e\u904e\u7a0b\u306b\u304a\u3051\u308bATP\u306e\u52a0\u6c34\u5206\u89e3\u306b\u3088\u308a\u3001MutS\u306fMutL\u306e\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u3092\u4ea2\u9032\u3059\u308b (2019\/03\/15 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>DNA\u30df\u30b9\u30de\u30c3\u30c1\u4fee\u5fa9\u306fMutS\u306b\u3088\u308b\u30df\u30b9\u30de\u30c3\u30c1\u5869\u57fa\u5bfe\u306e\u8a8d\u8b58\u3068MutL\u53ca\u3073\u4fee\u5fa9\u306b\u304b\u304b\u308f\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30ea\u30af\u30eb\u30fc\u30c8\u306b\u3088\u3063\u3066\u884c\u308f\u308c\u307e\u3059\u3002<\/li>\n<li>MutL\u306e\u975e\u7279\u7570\u7684DNA\u5207\u65ad\u306fATP\u7d50\u5408\u306b\u3088\u308a\u6291\u5236\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u306e\u6d3b\u6027\u5316\u6a5f\u69cb\u306f\u4e0d\u660e\u3067\u3057\u305f\u3002\u672c\u7814\u7a76\u306b\u304a\u3044\u3066\u7b46\u8005\u3089\u306fMutL\u306e\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u306eATP\u7d50\u5408\u4f9d\u5b58\u7684\u306a\u6291\u5236\u304cATP\u306e\u52a0\u6c34\u5206\u89e3\u306b\u3088\u3063\u3066\u89e3\u9664\u3055\u308c\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002<br \/>\n\u672c\u7814\u7a76\u3067\u306f\u3001\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305f MutS\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u30af\u30ed\u30fc\u30f3TEx18D08 (cat.# THR007280)<br \/>\n\u53ca\u3073 MutL\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u30af\u30ed\u30fc\u30f3TEx18C04 (cat.# THR007252) \u304cATPase assay\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nShimada A, Kawasoe Y, Hata Y, Takahashi TS, Masui R, Kuramitsu S, Fukui K. MutS stimulates the endonuclease activity of MutL in an ATP-hydrolysis-dependent manner. FEBS J. 280(14):3467-3479, 2013.  PMID : 23679952.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/THR007280\">TEx18D08 (Thermus thermophilus MutS)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/THR007252\">TEx18C04 (Thermus thermophilus MutL)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0056j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/> \u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u57f9\u990a\u7d30\u80de\u3067\u306e\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u7528\u30d7\u30e9\u30b9\u30df\u30c9(2019\/2\/22 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8S2\u7d30\u80de\u3092\u4f7f\u3063\u305f\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u7cfb\u306f\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u3053\u308c\u307e\u3067\u3082\u7b46\u8005\u3089\u304c\u69cb\u7bc9\u3057\u305fpMT-PURO (cat# RDB08532) \u304c\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u672c\u8a8c\u306b\u304a\u3044\u3066\u306f\u5b89\u5b9a\u767a\u73fe\u682a\u306e\u4f5c\u88fd\u3092\u3055\u3089\u306b\u52b9\u679c\u7684\u306b\u884c\u3046\u305f\u3081\u306b\u4ee5\u4e0b\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u69cb\u7bc9\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>1. pMT-PURO2 (cat# RDB12152): \u30d4\u30e5\u30fc\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u30de\u30fc\u30ab\u30fc\u306epuromycin N-acetyl-transferase (pac)\u3092\u5b89\u5b9a\u3057\u3066\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u306bpac\u907a\u4f1d\u5b50\u4e0a\u6d41\u306bkozak\u914d\u5217\u3092\u5c0e\u5165\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u3002<\/li>\n<li>2. pMT-PURO2G (cat# RDB09004): \u30d4\u30e5\u30fc\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u30de\u30fc\u30ab\u30fcpac\u306b\u878d\u5408\u3057\u305fEGFP\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u3067\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u3084\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u306e\u8a55\u4fa1\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>3. pMT-PURO2R (cat# RDB09005): \u30d4\u30e5\u30fc\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u30de\u30fc\u30ab\u30fcpac\u306b\u878d\u5408\u3057\u305fDsRED2\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u3067\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u3084\u907a\u4f1d\u5b50\u5c0e\u5165\u52b9\u7387\u306e\u8a55\u4fa1\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u76ee\u7684\u907a\u4f1d\u5b50\u306fmetallothionein \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u4e0b\u6d41\u306eMCS\u306b\u633f\u5165\u3057\u307e\u3059\u3002\u4f5c\u88fd\u3057\u305f\u5b89\u5b9a\u767a\u73fe\u682a\u306fCuSO4\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nNagahashi, K. et al. Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology. 65 (2): 173-178, 2013. PMID : 22706964.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08532\">pMT-PURO (cat# RDB08532)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12152\">pMT-PURO2 (cat# RDB12152)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09004\">pMT-PURO2G (cat# RDB09004)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09005\">pMT-PURO2R (cat# RDB09005)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0088j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u6d3b\u6027\u3092\u5b9a\u91cf\u7684\u306b\u8a55\u4fa1\u3067\u304d\u308b\u30d7\u30ed\u30fc\u30d6(2019\/2\/1 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30aa\u30fc\u30c8\u30d5\u30a1\u30b4\u30bd\u30fc\u30e0\u3092\u53ef\u8996\u5316\u3057\u3066\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3092\u89b3\u5bdf\u3059\u308b\u305f\u3081\u306bMAP1LC3 (LC3)\u3068\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u878d\u5408\u3055\u305b\u305f\u30d7\u30ed\u30fc\u30d6\u304c\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u9032\u884c\u306b\u4f34\u3044\u30d7\u30ed\u30fc\u30d6\u306e\u86cd\u5149\u304c\u6e1b\u8870\u3059\u308b\u305f\u3081\u3001\u5b9a\u91cf\u7684\u306a\u6d3b\u6027\u8a55\u4fa1\u306b\u306f\u4e0d\u5411\u304d\u3067\u3057\u305f\u3002<\/li>\n<li>\u6771\u4eac\u5927\u5b66\u306e\u6c34\u5cf6\u6607\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001LC3\u3068GFP\u3092\u878d\u5408\u3057\u305fGFP-LC3\u3068\u3001\u672b\u7aef\u306e\u30b0\u30ea\u30b7\u30f3 (G)\u3092\u6b20\u304fLC3\u3068RFP\u3092\u878d\u5408\u3057\u305fRFP-LC3\u0394G\u3092\u540c\u6642\u306b\u5f53\u30e2\u30eb\u91cf\u3067\u767a\u73fe\u3059\u308b\u30d7\u30ed\u30fc\u30d6\u3092\u958b\u767a\u3057\u307e\u3057\u305f<\/li>\n<li>\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u9032\u884c\u3088\u3063\u3066GFP-LC3\u306f\u86cd\u5149\u304c\u6e1b\u8870\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001RFP-LC3\u0394G\u306f\u7d30\u80de\u5185\u306b\u7559\u307e\u308b\u305f\u3081\u3001\u5185\u90e8\u6a19\u6e96\u3068\u3057\u3066\u6a5f\u80fd\u3057\u307e\u3059\u3002\u5373\u3061\u3001RFP\u306b\u5bfe\u3059\u308bGFP\u306e\u86cd\u5149\u5f37\u5ea6\u6bd4\u3068\u3057\u3066\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u6d3b\u6027\u3092\u5b9a\u91cf\u7684\u306b\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/rdb14600_300.png\" alt=\"\" \/><\/li>\n<li>\u672c\u30d7\u30ed\u30fc\u30d6\u306f\u3001\u57f9\u990a\u7d30\u80de\u3060\u3051\u3067\u306a\u304f\u3001\u30de\u30a6\u30b9\u3084\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u306e\u53d7\u7cbe\u5375\u3084\u7d44\u7e54\u3067\u306e\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u6d3b\u6027\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u591a\u691c\u4f53\u89e3\u6790\u306b\u3082\u9069\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKaizuka, T. et al. An Autophagic Flux Probe that Releases an Internal Control. Mol. Cell, 64 (4): 835-849, 2016. PMID: 27818143<\/li>\n<p><a href=\"http:\/\/www.m.u-tokyo.ac.jp\/news\/admin\/release_20161107.pdf\">\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u6d3b\u6027\u3092\u7c21\u4fbf\u304b\u3064\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3067\u304d\u308b\u65b0\u898f\u30d7\u30ed\u30fc\u30d6\u306e\u958b\u767a [\u6771\u4eac\u5927\u5b66\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9, 2016\/11\/7] [link]<\/a><\/li>\n<li>\u63d0\u4f9b\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14600\">pMRX-IP-GFP-LC3-RFP-LC3 delta G (cat# RDB14600)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0087j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>\u30aa\u30d7\u30c8\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\uff08\u5149\u907a\u4f1d\u5b66\uff09\u624b\u6cd5\u7528\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc(2019\/1\/11 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30c1\u30e3\u30cd\u30eb\u30ed\u30c9\u30d7\u30b7\u30f3\u30fb\u30b0\u30ea\u30fc\u30f3\u30ec\u30b7\u30fc\u30d0\u30fc\uff08ChRGR\uff09(cat# RDB16748)\u306f\u3001\u7dd1\u8272\u5149\u7167\u5c04\u306b\u3088\u308a\u819c\u96fb\u4f4d\u632f\u52d5\u304c\u8a98\u5c0e\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>ChRGR(ER) (cat# RDB16750)\u306f\u3001ChRGR\u3092\u5c0f\u80de\u4f53\/\u7b4b\u5c0f\u80de\u4f53\uff08ER\/SR\uff09\u306b\u5c40\u5728\u3055\u305b\u308b\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3067\u3042\u308a\u3001\u539f\u8457\u8ad6\u6587\u3067\u306f\u7d30\u80de\u5185\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u653e\u51fa\u306e\u64cd\u4f5c\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nAsano, T. Igarashi, H. Ishizuka, T. Yawo, H. Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12 :561, 2018. PMID: 30174581.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16748\">pCAGGS-ChRGR-Venus (cat# RDB16748)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16750\">pCAGGS-ChRGR(ER)-Venus (cat# RDB16750)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0057e\">Further Genetic Resources for Optgenetics Research<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0086j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>Cre\/loxP\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308amCherry\u767a\u73fe\u3092\u8a98\u5c0e\u3059\u308b\u30d9\u30af\u30bf\u30fc (2018\/12\/07 S.H.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u5927\u962a\u5927\u5b66\u7560\u4e2d\u7531\u7f8e\u5b50\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u3088\u308a\u3001Cre\/loxP\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308a\u8a98\u5c0e\u53ef\u80fd\u306amCherry\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u304c\u5230\u7740\u3057\u307e\u3057\u305f\u3002\u767a\u8868\u8ad6\u6587\u3067\u306f\u3001pCALNL5:mCherry\u3092\u4f4e\u6fc3\u5ea6\u306eCre\u7d44\u63db\u3048\u9175\u7d20\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3068\u5171\u306b\u30de\u30a6\u30b9\u306e\u767a\u751fE12.5\u9f62\u306eVZ\u9818\u57df\u3078\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u795e\u7d4c\u524d\u99c6\u7d30\u80de\u3092\u30af\u30ed\u30fc\u30ca\u30eb\u306b\u6a19\u8b58\u3057\u307e\u3057\u305f\u3002\u795e\u7d4c\u524d\u99c6\u7d30\u80de\u3068\u8ef8\u7d22\u69d8\u7a81\u8d77\u306fmCherry\u3067\u6a19\u8b58\u3055\u308c\u3066\u304a\u308a\u3001\u7d4c\u6642\u7684\u306a\u89b3\u5bdf\u306b\u3088\u308a\u3001\u8ef8\u7d22\u69d8\u7a81\u8d77\u306e\u6570\u3084\u9577\u3055\u306b\u3088\u308a\u7d30\u80de\u3092\u5206\u985e\u3057\u305d\u306e\u52d5\u614b\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nHatanaka, Y. and Yamauchi, K. Excitatory Cortical Neurons with Multipolar Shape Establish Neuronal Polarity by Forming a Tangentially Oriented Axon in the Intermediate Zone. Cerebral Cortex J., 23: 105, 2013. PMID 22267309.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB16747\">pCALNL5:mCherry (cat# RDB16747)<\/a><\/li>\n<li>Cre\u7d44\u63db\u3048\u9175\u7d20\u767a\u73fe\u30d9\u30af\u30bf\u30fc<br \/>\n\u7406\u7814DNA BANK\u3067\u306f<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0073e\">\u5404\u7a2eCre\u7d44\u63db\u3048\u9175\u7d20\u767a\u73fe\u30d9\u30af\u30bf\u30fc[link]<\/a>\u3092\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n<p><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>\u7d30\u80de\u5185\u5c0f\u5668\u5b98\u53ef\u8996\u5316\u30af\u30ed\u30fc\u30f3\u306e\u3054\u7d39\u4ecb (2018\/11\/14 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u5f53\u5ba4\u3067\u306f\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3084\u6838\u306a\u306915\u7a2e\u985e\u306e\u7d30\u80de\u5185\u5c0f\u5668\u5b98\uff08\u30aa\u30eb\u30ac\u30cd\u30e9\uff09\u3092\u53ef\u8996\u5316\u3067\u304d\u308b\u30af\u30ed\u30fc\u30f3\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u5404\u3005\u306e\u30af\u30ed\u30fc\u30f3\u306b\u306f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u30de\u30fc\u30ab\u30fc\u3084\u30a8\u30d4\u30c8\u30fc\u30d7\u30bf\u30b0\u3092\u878d\u5408\u3057\u305f\u30aa\u30eb\u30ac\u30cd\u30e9\u5c40\u5728\u30b7\u30b0\u30ca\u30eb\u914d\u5217\u304c\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u3066\u304a\u308a\u3001\u7d30\u80de\u5185\u3067\u767a\u73fe\u3055\u305b\u305f\u30de\u30fc\u30ab\u30fc\u306e\u86cd\u5149\u306b\u3088\u308b\u691c\u51fa\u3001\u3042\u308b\u3044\u306f\u3001\u6297\u4f53\u306b\u3088\u308b\u30bf\u30b0\u306e\u691c\u51fa\u306b\u3088\u3063\u3066\u30aa\u30eb\u30ac\u30cd\u30e9\u3092\u89b3\u5bdf\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u3053\u308c\u3089\u306e\u518512\u7a2e\u985e\u306f\u3001\u7406\u5316\u5b66\u7814\u7a76\u6240 \u751f\u547d\u6a5f\u80fd\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc (BDR) \u306e\u5ca1\u7530\u5eb7\u5fd7\u5148\u751f\u3001\u9ad8\u4e95\u5553\u5148\u751f\u3001\u5927\u962a\u5927\u5b66\u7523\u696d\u79d1\u5b66\u7814\u7a76\u6240\u306e\u6c38\u4e95\u5065\u6cbb\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u3063\u3066\u958b\u767a\u3055\u308c\u305fNano-lantern \u306b\u3088\u308b\u89b3\u5bdf\u304c\u53ef\u80fd\u3067\u3059 [Takai, A. et al., Proc Natl Acad Sci U S A. 112 (14): 4352-4356, 2015]\u3002Nano-lantern\u306f\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\uff0d\u30bc\u3068\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u878d\u5408\u3057\u305f\u3001\u5f93\u6765\u3088\u308a\u3082\u660e\u308b\u3044\u767a\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002\u30aa\u30eb\u30ac\u30cd\u30e9\u5c40\u5728\u30b7\u30b0\u30ca\u30eb\u6bce\u306b\u9ec4\u7dd1\u8272\u3001\u9752\u7dd1\u8272\u3001\u6a59\u8272\u306e3\u8272\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u7a4d\u6975\u7684\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0053e\">\u53ef\u8996\u5316\u30af\u30ed\u30fc\u30f3\u30ea\u30b9\u30c8<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0043j\">Nano-lantern \u30ea\u30b9\u30c8<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0081j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>Akaluc \u65b0\u898f\u958b\u767a\u306e\u8d64\u8272\u767a\u5149\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc (2018\/5\/7 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30db\u30bf\u30eb\u306e\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u751f\u547d\u73fe\u8c61\u306e\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u767a\u5149\u57fa\u8cea\u306e\u7d44\u7e54\u900f\u904e\u6027\u304c\u4f4e\u3044\u3068\u3044\u3046\u6b20\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u7406\u7814\u8133\u795e\u7d4c\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u5bae\u8107 \u6566\u53f2\u5148\u751f\u3001\u5ca9\u91ce \u667a\u5148\u751f\u3001\u96fb\u6c17\u901a\u4fe1\u5927\u5b66\u5927\u5b66\u306e\u7267 \u660c\u6b21\u90ce\u5148\u751f\u306e\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u751f\u304d\u305f\u52d5\u7269\u500b\u4f53\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u4eba\u5de5\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0AkaBLI\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>AkaBLI\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u7d44\u7e54\u900f\u904e\u6027\u306e\u5411\u4e0a\u3057\u305f\u4eba\u5de5\u57fa\u8ceaAkaLumine\u3068AkaLumine\u306b\u5408\u308f\u305b\u3066\u958b\u767a\u3057\u305f\u4eba\u5de5\u9175\u7d20Akaluc\u3067\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002AkaBLI\u306e\u767a\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5f37\u5ea6\u306f\u3001\u5f93\u6765\u3068\u6bd4\u3079100\uff5e1000\u500d\u3067\u3059\u3002<\/li>\n<li>\u5bae\u8107\u7814\u7a76\u5ba4\u3088\u308a\u3001\u4eba\u5de5\u9175\u7d20Akaluc\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u304c\u5f53\u907a\u4f1d\u5b50\u6750\u6599\u958b\u767a\u5ba4\u306b\u5bc4\u8a17\u3055\u308c\u3001\u63d0\u4f9b\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u7686\u69d8\u306e\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nIwano, S. et al., Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science 359 (6378): 935-939 (2018). PMID 29472486.<\/li>\n<li>\u30d7\u30ec\u30b9<br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2018\/20180223_1\/\">\u300c\u8133\u306e\u6df1\u90e8\u3092\u975e\u4fb5\u8972\u7684\u306b\u89b3\u5bdf\u3067\u304d\u308b\u4eba\u5de5\u751f\u7269\u767a\u5149\u30b7\u30b9\u30c6\u30e0AkaBLI\u300d<\/a> (2018\/2\/23 \u7406\u7814)<\/li>\n<li>DNA resource<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15781\">pcDNA3 Venus-Akaluc (cat# RDB15781)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15782\">pAAV2 SynTetOff Venus-Akaluc (cat# RDB15782)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15783\">pAAV2 TRE Venus-Akaluc (cat# RDB15783)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0082j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u5171\u751f\u5fae\u751f\u7269\u9593\u306e\u4ee3\u8b1d\u7d4c\u8def\u306e\u30b2\u30ce\u30e0\u89e3\u6790 (2018\/3\/2 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u5206\u679d\u9396\u8102\u80aa\u9178 (Branched-Chain Fatty Acid, BCFA) \u306e\u5171\u751f\u5206\u89e3\u306f\u3001\u5acc\u6c17\u74b0\u5883\u4e0b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u30a2\u30df\u30ce\u9178\u304b\u3089\u306e\u30e1\u30bf\u30f3\u7523\u751f\u306e\u4e0d\u53ef\u6b20\u306a\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002BCFA\u306e\u4ee3\u8b1d\u7d4c\u8def\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u3001\u5f53\u30bb\u30f3\u30bf\u30fc\u304b\u3089\u63d0\u4f9b\u3057\u305fBCFA\u4ee3\u8b1d\u53ef\u80fd\u306aJCM 14075T\u682a\u306e\u30b2\u30ce\u30e0DNA\u306b\u3064\u3044\u3066\u89e3\u6790\u3055\u308c\u3001\u3055\u3089\u306bBCFA\u4ee3\u8b1d\u4e0d\u53ef\u80fd\u306a\u83cc\u682a\u306e\u30c7\u30fc\u30bf\u3068\u306e\u6bd4\u8f03\u89e3\u6790\u304c\u884c\u308f\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nNarihiro, T. et al. Comparative Genomics of Syntrophic Branched-Chain Fatty Acid Degrading Bacteria. Microbes. Environ. 31 (3): 288-922 (2016). PMID 27431485.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/JGD12758\">Genomic DNA of Syntrophomonas wolfei subsp. methylbutyratica JCM 14075T (cat# JGD12758)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0080j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>\u30d3\u30aa\u30c1\u30f3\u5316\u30bf\u30b0\u4ed8\u52a0\u8ee2\u5199\u56e0\u5b50\u306b\u3088\u308b\u5b89\u5b9a\u3057\u305fChIP-seq\u89e3\u6790 (2018\/2\/16 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>ChIP-seq\u89e3\u6790\u306f\u3001\u7db2\u7f85\u7684\u306a\u8ee2\u5199\u56e0\u5b50\u7d50\u5408\u30b5\u30a4\u30c8\u89e3\u6790\u306e\u6709\u52b9\u306a\u624b\u6cd5\u306e\u4e00\u3064\u3067\u3059\u3002\u3057\u304b\u3057\u3001ChIP-seq\u89e3\u6790\u306e\u7cbe\u5ea6\u306f\u6297\u4f53\u306e\u54c1\u8cea\u306b\u4f9d\u5b58\u3057\u3066\u3044\u308b\u554f\u984c\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u672c\u8ad6\u6587\u3067\u306f\u3001\u30d3\u30aa\u30c1\u30f3\u5316\u30bf\u30b0\u3092\u8ee2\u5199\u56e0\u5b50\u306b\u4ed8\u52a0\u3057\u3066\u767a\u73fe\u3055\u305b\u3001\u30a2\u30d3\u30b8\u30f3\u30d3\u30fc\u30ba\u306b\u3088\u308a\u56de\u53ce\u3059\u308b\u3053\u3068\u3067\u3001ChIP-seq\u89e3\u6790\u306b\u9069\u3057\u305f\u6297\u4f53\u304c\u5f97\u3089\u308c\u306a\u3044\u5834\u5408\u3084\u591a\u7a2e\u985e\u306e\u8ee2\u5199\u56e0\u5b50\u3092\u5bfe\u8c61\u3068\u3059\u308b\u5834\u5408\u306b\u3001\u3088\u308a\u7c21\u4fbf\u3067\u5b89\u5b9a\u3057\u305fChIP-seq\u89e3\u6790\u304c\u3067\u304d\u308b\u3053\u3068\u3092\u5831\u544a\u3057\u3066\u3044\u307e\u3059<\/li>\n<li>\u8ad6\u6587<br \/>\nMatsuda, K. et al., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958, 2017. [PMID 28455373]<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15774\">pCAGGS-BLRP-BirA (cat# RDB15774)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0079j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>\u8102\u8cea\u30e9\u30d5\u30c8\u306e\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3059\u308b\u86cd\u5149\u6a19\u8b58\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u3054\u7d39\u4ecb (2018\/2\/6 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u8102\u8cea\u30e9\u30d5\u30c8\u306f\u3001\u7d30\u80de\u819c\u4e0a\u306e\u5fae\u5c0f\u9818\u57df\u3067\u3042\u308a\u3001\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3084\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u2015\u30b7\u30b9\u306a\u3069\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u3001\u8102\u8cea\u30e9\u30d5\u30c8\u3092\u69cb\u6210\u3059\u308b\u30b9\u30d5\u30a3\u30f3\u30b4\u30df\u30a8\u30ea\u30f3\u3084\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u3092\u500b\u3005\u306b\u6a19\u8b58\u3059\u308b\u624b\u6bb5\u304c\u4e4f\u3057\u304f\u3001\u8a73\u7d30\u306a\u89e3\u6790\u304c\u56f0\u96e3\u3067\u3057\u305f\u3002<\/li>\n<li>\u7406\u5316\u5b66\u7814\u7a76\u6240 \u5c0f\u6797\u8102\u8cea\u751f\u7269\u5b66\u7814\u7a76\u5ba4\u306e\u5c0f\u6797\u4fca\u79c0\u4e3b\u4efb\u7814\u7a76\u54e1\uff08\u7814\u7a76\u5f53\u6642\uff09\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u8102\u8cea\u306b\u7d50\u5408\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u878d\u5408\u3057\u3001\u30d7\u30ed\u30fc\u30d6\u3068\u3059\u308b\u3053\u3068\u3067\u8102\u8cea\u30e9\u30d5\u30c8\u3092\u53ef\u8996\u5316\u3059\u308b\u624b\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001\u3053\u308c\u3089\u306e\u8102\u8cea\u30e9\u30d5\u30c8\u3001\u30b9\u30d5\u30a3\u30f3\u30b4\u30df\u30a8\u30ea\u30f3\u3001\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u3092\u6a19\u8b58\u3067\u304d\u308bmCherry\u3042\u308b\u3044\u306fGFP\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<li>\u53c2\u8003Web\u30b5\u30a4\u30c8\u3001\u53c2\u8003\u6587\u732e<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GSB0027e\">\u8102\u8cea\u30e9\u30d5\u30c8\u6848\u5185\u30b5\u30a4\u30c8 [link]<\/a><br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2016\/20160822_2\/\">\u30de\u30a4\u30bf\u30b1\u7531\u6765\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u6b96\u3092\u6291\u5236 (RIKEN, 2016.8) [link]<\/a><br \/>\nMakino, A. et al. FASEB J., 31 (4): 1301-1322, 2017. [PMID: 27492925]<br \/>\nMakino, A. et al. FASEB J., 29 (2): 477-493, 2015. [PMID: 25389132]<br \/>\nShimada, Y. et al. Eur. J. Biochem., 269 (24): 6195-6203, 2002. [PMID: 12473115]<\/li>\n<\/ul>\n<p><a name=\"EXR0073j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> BAC\u30af\u30ed\u30fc\u30f3\u3092\u7528\u3044\u305fGpr151-Cre\u30de\u30a6\u30b9\u306e\u6a39\u7acb\u3068\u795e\u7d4c\u7d30\u80de\u306e\u7834\u58ca (2018\/02\/06 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> Gpr151\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u5236\u5fa1\u4e0b\u3067NLS-Cre\u3092\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u306eBAC\u30af\u30ed\u30fc\u30f3\u306e\u4f5c\u88fd\u306bMSMg01-081G04\u304c\u4f7f\u7528\u3055\u308c\u3001\u3053\u306e\u30af\u30ed\u30fc\u30f3\u3092\u7528\u3044\u3066Gpr151-Cre\u30de\u30a6\u30b9\u3092\u6a39\u7acb\u3057\u307e\u3057\u305f\u3002Cre\u7279\u7570\u7684lacZ\u30ec\u30dd\u30fc\u30bf\u30fc\u30de\u30a6\u30b9\u3068\u306e\u4ea4\u914d\u306b\u3088\u308a\u3001interpeduncular nucleus (IPN)\u306b\u6295\u5c04\u3059\u308b\u5185\u5074\u624b\u7db1\u6838\uff08MHb\uff09medial nucleus of habenular complex (mHb) \u30b3\u30ea\u30f3\u4f5c\u52d5\u6027\u795e\u7d4c\u7d30\u80de\u3092\u6a19\u7684\u3068\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001Eno2-Diphtheria toxin A \u30de\u30a6\u30b9\u3068\u306e\u639b\u3051\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u7279\u5b9a\u306e\u795e\u7d4c\u7d30\u80de\u306e\u7834\u58ca\u306b\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKobayashi Y. et al., Genetic dissection of medial habenula-interpeduncular nucleus pathway function in mice. Front Behav. Neurosci. 7: 17 (2013). PMID: 23487260.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0039e\">MSM\/Ms Mouse BAC clone<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB04214\">MSM\/Ms mouse BAC references<\/a><br \/>\n<a href=\"http:\/\/analysis2.lab.nig.ac.jp\/mouseBrowser\/cgi-bin\/bacendInfo.cgi?&#038;org=mm&#038;id=MSMg01-081G04\">MSMg01-081G04 (searching result)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0075j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u5206\u88c2\u9175\u6bcd\u3092\u7528\u3044\u305f\u70ad\u75bd\u83cc\u7d30\u80de\u9aa8\u683c\u306e\u89e3\u6790 (2018\/02\/06 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> \u30d0\u30af\u30c6\u30ea\u30a2\u7d30\u80de\u9aa8\u683c\u306f\u75c5\u539f\u539f\u6838\u751f\u7269\u304c\u3082\u3064\u75c5\u539f\u6027\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5206\u914d\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u70ad\u75bd\u83ccBacillus anthracis\u306eTubZ (Ba-TubZ)\u306fpXO1\u306e\u5206\u914d\u306b\u95a2\u308f\u3063\u3066\u3044\u308b\u304c\u3001\u751f\u7269\u7269\u7406\u5b66\u7684\u7279\u6027\u306f\u8a73\u7d30\u306b\u89e3\u6790\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u305d\u306e\u7279\u6027\u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u3001GFP\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u88c2\u9175\u6bcd\u306b\u767a\u73fe\u3055\u305b\u3001\u5171\u7126\u70b9\u9855\u5fae\u93e1\u753b\u50cf\u304c\u89e3\u6790\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nSrinivasan, R., Mishra, M., Leong, F.Y., Chiam, K.H., Balasubramanian, M. Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers. Cytoskeleton (Hoboken) 68 (9): 501-511 (2011). PMID 21780309.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06133\">pDUAL-HFG1c (cat# RDB06133)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06134\">pDUAL-HFG41c (cat# RDB06134)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06526\">pHFG41-ccdB2 (cat# RDB06526)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0074j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Gli\u8a8d\u8b58\u914d\u5217\u30ec\u30dd\u30fc\u30bf\u30fc\u3092\u7528\u3044\u305fHedgehog\u30b7\u30b0\u30ca\u30eb\u306e\u89e3\u6790 (2018\/02\/06 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> \u9aa8\u7c97\u3057\u3087\u3046\u75c7\u306e\u8a98\u767a\u3068Hedgehog\u30b7\u30b0\u30ca\u30eb\u306e\u6291\u5236\u306b\u304a\u3051\u308b\u9178\u5316\u30b9\u30c8\u30ec\u30b9\u306e\u5f71\u97ff\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u306b\u3001\u30de\u30a6\u30b9\u80da\u7531\u6765\u9593\u8449\u7cfbC3H10T1\/2\u7d30\u80de\u306e\u9aa8\u82bd\u7d30\u80de\u5206\u5316 \u306b\u3064\u3044\u3066\u3001\u4ecb\u5728\u56e0\u5b50\u3068\u60f3\u5b9a\u3055\u308c\u3066\u3044\u308bMAPK\u306b\u6ce8\u76ee\u3057\u3066\u89e3\u6790\u3057\u307e\u3057\u305f\u3002C3H10T1\/2\u7d30\u80de\u306e\u9178\u5316\u30b9\u30c8\u30ec\u30b9\u3068MAPK\u30a4\u30f3\u30d2\u30d3\u30bf\u30fc\u306e\u5f71\u97ff\u4e0b\u3067Gli\u3092\u4ecb\u3057\u305f\u8ee2\u5199\u6d3b\u6027\u5316\u3092\u89b3\u5bdf\u3059\u308b\u305f\u3081\u306b\u3001Gli\u8ee2\u5199\u56e0\u5b50\u8a8d\u8b58\u914d\u5217\u3092\u6301\u3064\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30ec\u30dd\u30fc\u30bf\u30fc\u304c\u4f7f\u7528\u3055\u308c\u3001JNK1\u306b\u3088\u308bGli\u3092\u4ecb\u3057\u305f\u8ee2\u5199\u6d3b\u6027\u5316\u306e\u6291\u5236\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nShiohama, T., Fujii, K., Uchikawa, H., Takatani, T., Mizuochi, H., Kohno, Y. Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2): 53-62 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08061\">8&#215;3&#8217;Gli-BS-delta51-LucII (cat# RDB08061)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08062\">8xm3&#8217;Gli-BS-delta51-LucII (cat# RDB08062)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08064\">pcDNA3.1-His-Gli1 (cat# RDB08064)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0077j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30d2\u30c8iPS\u7d30\u80de\u3092\u809d\u7d30\u80de\u306b\u5206\u5316\u8a98\u5c0e\u3059\u308b\u907a\u4f1d\u5b50 (2018\/02\/06 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> iPS\u7d30\u80de\u3092\u809d\u7d30\u80de\u306b\u5206\u5316\u8a98\u5c0e\u3059\u308b\u5019\u88dc\u907a\u4f1d\u5b50\u3092\u898b\u3064\u3051\u308b\u305f\u3081\u3001\u30d2\u30c8\u6210\u4eba\u809d\u7d30\u80de\u3068iPS\u7d30\u80de201B7\u7d30\u80de\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u6bd4\u8f03\u306b\u3088\u308a\u8ee2\u5199\u56e0\u5b50\u3092\u7d5e\u308a\u8fbc\u307f\u300116\u306e\u907a\u4f1d\u5b50\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u308916\u7a2e\u985e\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092\u305d\u308c\u305e\u308c\u69cb\u7bc9\u3001iPS\u7d30\u80de\u304b\u3089\u809d\u7d30\u80de\u5206\u5316\u3092\u8a98\u5c0e\u3059\u308b\u5019\u88dc\u907a\u4f1d\u5b50\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u3001201B7\u7d30\u80de\u306b\u5404\u30d9\u30af\u30bf\u30fc\u3092\u907a\u4f1d\u5b50\u5c0e\u5165\u3057\u3001\u672a\u6210\u719f\u809d\u7d30\u80de\u306e\u30de\u30fc\u30ab\u30fc\u3067\u3042\u308bAFP\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u8a98\u5c0e\u3057\u3001\u304b\u3064Nanog\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u6291\u5236\u3059\u308b\u907a\u4f1d\u5b50\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u3001CEBPA\u3001CEBPB\u3001FOXA1\u3001FOXA3\u306e4\u907a\u4f1d\u5b50\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u306e\u3046\u3061\u3001CEBPA\u3042\u308b\u3044\u306fCEBPB\u3042\u308b\u3044\u306f4\u907a\u4f1d\u5b50\u3092\u540c\u6642\u306b\u3092\u907a\u4f1d\u5b50\u5c0e\u5165\u3057\u305f\u7d30\u80de\u3067\u306fAFP\u304a\u3088\u3073\u30a2\u30eb\u30d6\u30df\u30f3\u306e\u6700\u3082\u9ad8\u3044\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u3001\u3053\u308c\u30894\u907a\u4f1d\u5b50\u304ciPS\u7d30\u80de\u306e\u809d\u7d30\u80de\u5206\u5316\u306b\u9069\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nTomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., Ishige, N. Transcription Factors and Medium Suitable for Initiating the Differentiation of Human-Induced Pluripotent Stem Cells to the Hepatocyte Lineage. J. Cell. Biochem. 117 (9): 2001-2009 (2016). Pubmed ID: 26773721.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\nGenome Network Project Clone<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGY121558\">IRCB003O22 (cat# HGY121558)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGY019155\">IRAK047O19 (cat# HGY019155)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGX006064\">IRAK015C16 (cat# HGX006064)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGY092741\">IRAL031O05 (cat# HGY092741)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGY019431\">IRAK048J15 (cat# HGY019431)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGX010994\">IRAK027I02 (cat# HGX010994)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGE029522\">W01A073N10 (cat# HGE029522)<\/a><br \/>\nNRCD Human cDNA Clone<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HKR042945\">Are07G01 (cat# HKR042945).<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0078j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> SF-1\u5f37\u5236\u767a\u73fe\u306b\u3088\u308b\u30b9\u30c6\u30ed\u30a4\u30c9\u7523\u751f\u7d30\u80de\u306e\u5206\u5316\u8a98\u5c0e (2018\/02\/06 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> 2012\u5e74\u306b\u3001SF-1\u767a\u73fe\u30af\u30ed\u30fc\u30f3pCMFlag_hsNR5A1 (RDB06299)\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u30d2\u30c8ES\u306a\u3089\u3073\u306biPS\u7d30\u80de\u304b\u3089\u4e2d\u80da\u8449\u5206\u5316\u3092\u7d4c\u3066\u30b9\u30c6\u30ed\u30a4\u30c9\u7523\u751f\u7d30\u80de\u306b\u5206\u5316\u3055\u305b\u308b\u624b\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f (Sonoyama et al., 2012)\u3002\u4eca\u56de\u3001\u30d2\u30c8iPS\u7d30\u80de\u7531\u6765\u306e\u521d\u671f\u4e2d\u9593\u4e2d\u80da\u8449\u306b\u540c\u767a\u73fe\u30af\u30ed\u30fc\u30f3\u3092\u5c0e\u5165\u3001dopamine D1 \u53d7\u5bb9\u4f53\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u306b\u3088\u308b\u30b9\u30c6\u30ed\u30a4\u30c9\u7523\u751f\u7d30\u80de\u306e\u8a98\u5c0e\u3092\u691c\u8a3c\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nMatsuo, K. et al., Significance of dopamine D1 receptor signaling for steroidogenic differentiation of human induced pluripotent stem cells. Sci. Rep. 7 (1): 15120 (2017). PMID: 29123220.<br \/>\nSonoyama, T. et al., Differentiation of human embryonic stem cells and human induced pluripotent stem cells into steroid-producing cells. Endocrinology 153 (9): 4336-4345 (2012). PMID: 22778223.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06299\">pCMFlag_hsNR5A1 (cat# RDB06299)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0076j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> ER\u30b9\u30c8\u30ec\u30b9\u306b\u3088\u308bATF6\u306b\u4f9d\u5b58\u3057\u305fSESN2\u306e\u767a\u73fe\u8a98\u5c0e (2018\/02\/06 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> SESN2\u306f\u69d8\u3005\u306a\u30b9\u30c8\u30ec\u30b9\u306b\u3088\u308a\u8a98\u5c0e\u3055\u308c\u3001\u7d30\u80de\u306e\u30b9\u30c8\u30ec\u30b9\u306b\u5bfe\u3059\u308b\u9069\u5fdc\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002ER\u30b9\u30c8\u30ec\u30b9\u306b\u3088\u308a\u8a98\u5c0e\u3055\u308c\u308bSESN2\u306eATF6\u306b\u4f9d\u5b58\u3057\u305f\u767a\u73fe\u306e\u89e3\u6790\u306e\u305f\u3081\u3001\u30c9\u30df\u30ca\u30f3\u30c8\u30cd\u30ac\u30c6\u30a3\u30d6\u578b\u3042\u308b\u3044\u306fsiRNA\u306b\u3088\u308bATF6\u306e\u6a5f\u80fd\u6291\u5236\u4e0b\u3067pGL4-phSESN2 (RDB07413) \u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nJegal, K.H. et al., Activating transcription factor 6-dependent sestrin 2 induction ameliorates ER stress-mediated liver injury. Biochim. Biophys. Acta 1864 (7): 1295-1307 (2017). PMID 28433684.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07413\">pGL4-phSESN2 (cat# RDB07413)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0079j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>&nbsp;\u86cd\u5149\u7d30\u80de\u5468\u671f\u30a4\u30f3\u30b8\u30b1\u30fc\u30bf\u30fcFucci\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u63d0\u4f9b\u306e\u3054\u6848\u5185 (2017\/11\/15 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u7814 \u8133\u795e\u7d4c\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08\u7406\u7814CBS\uff09\u306e\u5bae\u8107\u6566\u53f2\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u7d30\u80de\u5468\u671f\u3092\u751f\u304d\u305f\u307e\u307e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u89b3\u5bdf\u3067\u304d\u308b\u86cd\u5149\u7d30\u80de\u5468\u671f\u30a4\u30f3\u30b8\u30b1\u30fc\u30bf\u30fcFucci (Fluorescent Ubiquitination-based Cell Cycle Indicator)\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u5f53\u958b\u767a\u5ba4\u3067\u306f\u3001\u7d30\u80de\u5c0e\u5165\u7528\u3001\u30de\u30a6\u30b9R26\u907a\u4f1d\u5b50\u5ea7\u30bf\u30fc\u30b2\u30c3\u30c8\u7528\u3001\u4e26\u3073\u306b\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u7cfb\u7d71\u4f5c\u88fd\u7528\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002Fucci\u3092\u5c0e\u5165\u3057\u305f\u30de\u30a6\u30b9\u7cfb\u7d71\u3001\u7d30\u80de\u682a\u3082\u4f75\u305b\u3066\u3054\u5229\u7528\u304f\u3060\u3055\u3044\u3002\u6d3b\u767a\u306a\u3054\u5229\u7528\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002\n<li>\u53c2\u8003Web\u30b5\u30a4\u30c8\u3001\u53c2\u8003\u6587\u732e<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0044j\">Fucci \u6848\u5185\u30b5\u30a4\u30c8 [link]<\/a><br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2017\/20171027_1\/\">\u7d30\u80de\u5468\u671f\u306e\u9593\u671f\uff08G1\u30fbS\u30fbG2\uff09\u30923\u8272\u3067\u8b58\u5225\u3059\u308b\u6280\u8853\u306e\u958b\u767a (RIKEN, 2017) [link]<\/a><br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2011\/20110125\/\">\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u6280\u8853\u306b\u3088\u3063\u3066\u6297\u304c\u3093\u5264\u306e\u4f5c\u7528\u3092\u518d\u8a55\u4fa1 (RIKEN, 2011) [link]<\/a><br \/>\n<a href=\"http:\/\/www.jst.go.jp\/pr\/announce\/20080208\/index.html\">DNA\u8907\u88fd\u3084\u7d30\u80de\u5206\u88c2\u306e\u69d8\u5b50\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u89b3\u5bdf\u3059\u308b\u65b0\u6280\u8853 (JST, 2008) [link]<\/a><br \/>\nSakaue-Sawano, A. et al. Mol. Cell 68 (3): 626-640.e5, 2017. [PMID: 29107535]<br \/>\nMort, R.L. et al. Cell Cycle 13 (17): 2681-2696 ,2014. [PMID: 25486356]<br \/>\nSakaue-Sawano, A. et al. BMC. Cell Biol. 12: 2, 2011. [PMID: 21226962]<br \/>\nSugiyama, M. et al. Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817, 2009. [PMID: 19923430]\n<\/li>\n<\/ul>\n<p><a name=\"EXR0072j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u7523\u751f\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u3092\u5c0e\u5165\u3067\u304d\u308b\u5bbf\u4e3b\u5927\u8178\u83cc\u682a (2017\/07\/25 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li> \u5bbf\u4e3b\u5927\u8178\u83cc\u682aRFzero-iy\u682a\u4e26\u3073\u306bB-95.\u0394A\u682a\u306f\u3001\u5927\u8178\u83cc\u306eUAG\u30b3\u30c9\u30f3\u306b\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u3092\u5272\u308a\u5f53\u3066\u308b\u3053\u3068\u3067\u3001\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u3092\u53d6\u308a\u8fbc\u3093\u3060\u975e\u5929\u7136\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7523\u751f\u3067\u304d\u308b\u5bbf\u4e3b\u5927\u8178\u83cc\u682a\u3067\u3059\u3002<\/li>\n<li>\u5927\u8178\u83ccRFzero-iy \u682a\u306f\u3001\u7523\u751f\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u30013-\u30e8\u30fc\u30c9\u30c1\u30ed\u30b7\u30f3\u304c\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u305f\u5927\u8178\u83cc\u682a\u3067\u3059\u3002\u53d6\u308a\u8fbc\u307f\u305f\u3044\u90e8\u4f4d\u306bUGA\u30b3\u30c9\u30f3\u3092\u633f\u5165\u3057\u305f\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u672c\u682a\u306b\u5c0e\u5165\u3057\u30013-\u30e8\u30fc\u30c9\u30c1\u30ed\u30b7\u30f3\u3092\u6dfb\u52a0\u3057\u305f\u57f9\u5730\u3067\u57f9\u990a\u3057\u307e\u3059 (Mukai, T. et al., 2011) \u3002 \u5927\u8178\u83ccBW25113\u682a\u4e26\u3073\u306b\u5927\u8178\u83ccBL21(DE3)\u682a\u3092\u57fa\u306b\u3057\u305f\u682a\u304c\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u5927\u8178\u83ccB-95. \u0394A \u682a\u306f\u3001\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u7528tRNA\u3068\u30a2\u30df\u30ce\u30a2\u30b7\u30eb\u5408\u6210\u9175\u7d20\u3092\u30b3\u30fc\u30c9\u3059\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u203b\u306e\u5c0e\u5165\u306b\u3088\u308a\u3001UAG\u30b3\u30c9\u30f3\u306b\u4efb\u610f\u306e\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u3092\u5272\u308a\u5f53\u3066\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u672c\u682a\u3092\u7528\u3044\u3066\u3001\u6297\u8840\u6db2\u51dd\u56fa\u6d3b\u6027\u306e\u52b9\u679c\u5411\u4e0a\u304c\u671f\u5f85\u3055\u308c\u308b\u786b\u9178\u30c1\u30ed\u30b7\u30f3\u3092\u5c0e\u5165\u3057\u305f\u786b\u9178\u5316\u30d2\u30eb\u30b8\u30f3\u306e\u5408\u6210\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\uff08Mukai, T. et al., 2015\uff09\u3002\u30aa\u30ea\u30b8\u30ca\u30eb\u682a\u3068\u5897\u6b96\u80fd\u3092\u6539\u5584\u3057\u305f\u6d3e\u751f\u682a\u304c\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nMukai, T. et al., Biochem. Biophys. Res. Commun. 411 (4): 757-761, 2011. [PMID 21782790]<br \/>\nMukai, T. et al., Sci. Rep. 5: 9699, 2015. [PMID 25982672]\n<\/li>\n<li>\u53c2\u8003Web\u30b5\u30a4\u30c8<br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2015\/20150518_1\/\">DNA\u60c5\u5831\u306e\u5909\u63db\u30eb\u30fc\u30eb\u3092\u4eba\u70ba\u7684\u306b\u6539\u5909<\/a><br \/>\n<a href=\"http:\/\/www.clst.riken.jp\/ja\/science\/tech\/synthetic_amino_acid\/\">\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3078\u306e\u90e8\u4f4d\u7279\u7570\u7684\u5c0e\u5165\u6280\u8853<\/a>\n<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14427\">BW25113-based RFzero-iy (cat # RDB14427)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14428\">BL21(DE3)-based RFzero-iy (cat # RDB14428)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB13711\">B-95.\u0394A (cat # RDB13711)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB13712\">B-95.\u0394A\u0394fabR (cat # RDB13712)<\/a><br \/>\n\u203b\u4eba\u5de5\u30a2\u30df\u30ce\u9178\u5c0e\u5165\u7528\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u3001\u7406\u7814CLST\u304b\u3089\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n<p><a name=\"EXR0071j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u300cGAPDH-HT\u30a4\u30f3\u30b8\u30b1\u30fc\u30bf\u30fc\u300d\u306b\u3088\u308b\u30b7\u30e3\u30da\u30ed\u30f3\u4ecb\u5728\u6027\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\uff08CMA\uff09\u6d3b\u6027\u8a55\u4fa1(2017\/06\/30 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u300cGAPDH-HT\u30a4\u30f3\u30b8\u30b1\u30fc\u30bf\u30fc\u300d\u306f\u3001CMA\u306e\u4ee3\u8868\u7684\u306a\u57fa\u8cea\u3067\u3042\u308bGAPDH\u306bHaloTag\u3092\u878d\u5408\u3055\u305b\u305fCMA\u30de\u30fc\u30ab\u30fc\u3067\u3059\u3002\u86cd\u5149\u6a19\u8b58\u3057\u305fHaloTag\u30ea\u30ac\u30f3\u30c9\u3068\u5171\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u86cd\u5149\u9855\u5fae\u93e1\u3067\u7d30\u80de\u5185\u306eCMA\u6d3b\u6027\u3092\u7c21\u4fbf\u306b\u8a55\u4fa1\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nSeki, T. et al. Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron. PLoS One 7 (2): e31232, 2012. PMID: 22363588<\/p>\n<p>Sato, M. et al. Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes Cells 21 (8): 861-873, 2016. PMID: 27377049\n<\/p><\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB15088\">GAPDH-HT\/pcDNA5\/FRT (cat # RDB15088)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0066j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Cre\u7d44\u63db\u3048\u9175\u7d20\u3068flox\u30de\u30a6\u30b9\u7531\u6765\u7d30\u80de\u3092\u7528\u3044\u305f\u907a\u4f1d\u5b50\u6b20\u640d\u30e2\u30c7\u30eb\u7d30\u80de\u306e\u6a39\u7acb(2017\/06\/23 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>Has2\u907a\u4f1d\u5b50\u6b20\u640d\u30e2\u30c7\u30eb\u7d30\u80de\u3092\u4f5c\u88fd\u3059\u308b\u305f\u3081\u306b\u3001Cre recombinase \u767a\u73fe\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9AxCANCre\u304c\u5229\u7528\u3055\u308c\u307e\u3057\u305f\u3002Has2\u907a\u4f1d\u5b50\u306eexon 2\u3092loxP\u7d44\u63db\u3048\u914d\u5217\u3067\u631f\u3093\u3060flox\u30de\u30a6\u30b9\u304b\u3089\u7d30\u80de\u3092\u5206\u96e2\u3057\u3001\u3053\u306e\u7d30\u80de\u306b\u7d44\u63db\u3048\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9AxCANCre\u3092\u611f\u67d3\u3055\u305b\u308b\u3053\u3068\u3067exon 2\u6b20\u640d\u3055\u305b\u3001\u6b20\u640d\u30e2\u30c7\u30eb\u7d30\u80de\u3092\u6a39\u7acb\u3057\u3066\u3044\u307e\u3059<\/li>\n<li>\u8ad6\u6587<br \/>\nChanmee, T. et al. Hyaluronan production regulates metabolic and cancer stem-like properties of breast cancer cells via hexosamine biosynthetic pathway-coupled HIF-1 signaling. J. Biol. Chem. 291 (46): 24105-24120, 2016. PMID: 27758869.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01748\">AxCANCre (cat# RDB01748)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0070j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u53ef\u8996\u5316\u30c4\u30fc\u30eb\u300cM-INK\u300d(2017\/05\/02 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30e1\u30e9\u30ce\u30b5\u30a4\u30c8\u3084\u30b1\u30e9\u30c1\u30ce\u30b5\u30a4\u30c8\u5185\u306e\u30e1\u30e9\u30ce\u30bd\u30fc\u30e0\u3092\u53ef\u8996\u5316\u3067\u304d\u308b\u65b0\u898f\u30c4\u30fc\u30eb\u300cM-INK\u300d\u304c\u5230\u7740\u3057\u307e\u3057\u305f<\/li>\n<li>M-INK\u306e\u958b\u767a\u306b\u3088\u308a\u3001\u30e1\u30e9\u30ce\u30b5\u30a4\u30c8\u304b\u3089\u30b1\u30e9\u30c1\u30ce\u30b5\u30a4\u30c8\u306b\u53d7\u3051\u6e21\u3055\u308c\u308b\u30e1\u30e9\u30ce\u30bd\u30fc\u30e0\u3092\u52b9\u7387\u3088\u304f\u89b3\u5bdf\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nIshida, M. et al. M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem., 2017, in press.  PMID: 28096452<br \/>\nIshida, M. et al. Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep., 5: 8238, 2015.   PMID: 25649263<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"http:\/\/www.tohoku.ac.jp\/japanese\/newimg\/pressimg\/tohokuuniv-press20170119_01web.pdf\">\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u3092\u53ef\u8996\u5316\u3059\u308b\u65b0\u898f\u30c4\u30fc\u30eb\uff08M-INK(\u30a8\u30e0 \u30a4\u30f3\u30af)\uff09\u3092\u958b\u767a\uff08\u6771\u5317\u5927\u5b66\u5927\u5b66\u9662\u751f\u547d\u79d1\u5b66\u7814\u7a76\u6240 2017\u5e741\u670819 \u65e5\uff09<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14634\">pEF-T7-GST-Kif1c-tail-874-1100\/M-INK (cat.# RDB14634)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14632\">pEF-Myc-Kif1c-tail-365-843 (cat.# RDB14632)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14633\">pEF-Myc-Kif1c-tail-879-1100 (cat.# RDB14633)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14635\">pEF-Tyrosinase-FLAG (cat.# RDB14635)<\/a>\n<\/li>\n<\/ul>\n<p><a name=\"EXR0067j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u86cd\u5149\u30ab\u30eb\u30b7\u30a6\u30e0\u30bb\u30f3\u30b5\u30fc G-CaMP (2017\/03\/16 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7d30\u80de\u5185\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u306e\u86cd\u5149\u30bb\u30f3\u30b5\u30fc\u30bf\u30f3\u30d1\u30af\u8cea\u300cG-CaMP\u300d\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u304c\u5230\u7740\u3057\u307e\u3057\u305f\u3002\n<li>G-CaMP\u306f\u3001\u57fc\u7389\u5927\u5b66 \u8133\u672b\u68a2\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u4e2d\u4e95\u6df3\u4e00\u5148\u751f\u7b49\u306e\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u3063\u3066\u958b\u767a\u3055\u308c\u305f\u7d30\u80de\u5185\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u305f\u3081\u306e\u86cd\u5149\u30a4\u30f3\u30b8\u30b1\u30fc\u30bf\u30fc\u3067\u3059\u3002\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30ab\u30eb\u30e2\u30b8\u30e5\u30ea\u30f3\uff08Calmodulin; CaM\uff09\u30ab\u30eb\u30b7\u30a6\u30e0\u7d50\u5408\u9818\u57df\u3068\u30df\u30aa\u30b7\u30f3\u8efd\u9396\u30ad\u30ca\u30fc\u30bc\u306eM13\u65ad\u7247\u3092\u7d50\u5408\u3055\u305b\u305f\u30e6\u30cb\u30fc\u30af\u306a\u69cb\u9020\u3092\u3057\u3066\u304a\u308a\u3001\u30ab\u30eb\u30b7\u30a6\u30e0\u306e\u7d50\u5408\u306b\u3088\u308a\u8d77\u304d\u308b\u7acb\u4f53\u69cb\u9020\u5909\u5316\u306b\u3088\u308a\u86cd\u5149\u3092\u767a\u3057\u307e\u3059 (Nakai, J. et al., 2001)\u3002<\/li>\n<li>\u305d\u306e\u5f8c\u3001\u30aa\u30ea\u30b8\u30ca\u30eb\u306eG-CaMP\u306f\u3001\u81f3\u9069\u6e29\u5ea6\u306e\u62e1\u5145\u3084\u30b7\u30b0\u30ca\u30eb\/\u30ce\u30a4\u30ba\u6bd4\u3001\u611f\u53d7\u6027\u7b49\u306e\u5411\u4e0a\u306a\u3069\u306e\u6539\u826f\u304c\u52a0\u3048\u3089\u308c\u307e\u3057\u305f\u3002\u6539\u826f\u578bG-CaMP\u306f\u3001\u4ee3\u8868\u7684\u306a\u86cd\u5149\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30f3\u30b8\u30b1\u30fc\u30bf\u30fc\u3068\u3057\u3066\u4e16\u754c\u4e2d\u306e\u7814\u7a76\u8005\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002G-CaMP\u3092\u5229\u7528\u3057\u3066\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u3092\u89b3\u5bdf\u3057\u305f\u8ad6\u6587\u306f\u3001iPS\u7d30\u80de\u304b\u3089\u5206\u5316\u3055\u305b\u305f\u5fc3\u7b4b\u7d30\u80de\uff08Shiba, Y. et al., 2016; cat.no. RDB14612 G-CaMP7.09\uff09\u3084\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u306e\u795e\u7d4c\u7d30\u80de\uff08Muto, A. et al., 2011; cat.no. RDB14607 G-CaMP-HS\uff09\u7b49\u3001\u591a\u6570\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\n<\/li>\n<li>\u8ad6\u6587<br \/>\nShiba, Y. et al. Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature, 538 (7625): 388-391, 2016. PMID: 27723741<br \/>\nOhkura, M. et al. Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One, 7 (12): e51286, 2012. PMID: 23240011<br \/>\nOhkura, M. et al. An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One, 7 (7): e39933, 2012. PMID: 22808076<br \/>\nMuto, A. et al. Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U S A., 108 (13): 5425-5430, 2011.  PMID: 21383146<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14606\">G-CaMP4.1 (cat.# RDB14606)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14607\">G-CaMP-HS (cat.# RDB14607)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14609\">G-CaMP6 (cat.# RDB14609)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14610\">G-CaMP7 (cat.# RDB14610)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14611\">G-CaMP8 (cat.# RDB14611)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14612\">G-CaMP7.09 (cat.# RDB14612)<\/a><\/p>\n<p>Please also visit <a href=\"https:\/\/brc.riken.jp\/dna\/GRP0029e\">Calcium Ion Sensor Clones<\/a>.\n<\/li>\n<\/ul>\n<p><a name=\"EXR0065j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> BAC\u30af\u30ed\u30fc\u30f3\u3068recombineering \u624b\u6cd5\u306b\u3088\u308b\u9577\u3044\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u6301\u3064\u30ec\u30dd\u30fc\u30bf\u30fc\u306e\u69cb\u7bc9(2017\/02\/28 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3092\u4f7f\u7528\u3057\u305f\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4\u306f\u751f\u304d\u305f\u7d30\u80de\u3067\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u89b3\u5bdf\u306e\u5f37\u529b\u306a\u30c4\u30fc\u30eb\u3067\u3059\u3002\u3067\u304d\u308b\u3060\u3051\u9577\u3044\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u4f7f\u7528\u3057\u305f\u30ec\u30dd\u30fc\u30bf\u30fc\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u69cb\u7bc9\u3059\u308b\u3053\u3068\u304c\u671b\u307e\u308c\u307e\u3059\u3002\u4e00\u822c\u7684\u306a\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u65b9\u6cd5\u3067\u306f\u305d\u306e\u3088\u3046\u306a\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u69cb\u7bc9\u306f\u56f0\u96e3\u3067\u3059\u3002\u672c\u8ad6\u6587\u3067\u306f\u3001BAC\u30af\u30ed\u30fc\u30f3\u3068recombineering \u624b\u6cd5\u306b\u3088\u308aHprt\u907a\u4f1d\u5b50\u306e20 kb\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u6301\u3064\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30ec\u30dd\u30fc\u30bf\u30fc\u3092\u69cb\u7bc9\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nEndo, T. et al. Evaluation of an Hprt-luciferase reporter gene on a mammalian artificial chromosome in response to cytotoxicity. Yonago Acta Med. 59 (2): 174-182  PMID: 27493490.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"\/ja\/clonesetja\/nbrpb6nbacja\">C57BL\/6N (B6N) mouse BAC clone<\/a><br \/>\n<a href=\"http:\/\/analysis2.lab.nig.ac.jp\/mouseBrowser\/cgi-bin\/halDetail.cgi?org=mm&#038;chr=X&#038;level=6&#038;center=50441188&#038;keyword=B6Ng01-126E09\">Mouse B6N BAC clone B6Ng01-126E09 (\u30af\u30ed\u30fc\u30f3\u691c\u7d22\u7d50\u679c)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0064j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u6d3b\u6027\u3092\u5b9a\u91cf\u3067\u304d\u308b\u30d7\u30ed\u30fc\u30d6(2017\/02\/21 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>GFP\u3068RFP\u306e\u86cd\u5149\u5f37\u5ea6\u6bd4\u3092\u6307\u6a19\u3068\u3057\u3066\u3001\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u6d3b\u6027\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u30d7\u30ed\u30fc\u30d6\u304c\u5230\u7740\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u539f\u8457\u8ad6\u6587\u3067\u306f\u3001\u30de\u30a6\u30b9\u7e4a\u7dad\u82bd\u7d30\u80de\u3001HeLa\u7d30\u80de\u3001\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u80da\u3067\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u3092\u89b3\u5bdf\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKaizuka, T. et al. An Autophagic Flux Probe that Releases an Internal Control. Mol. Cell, 64 (4): 835-849, 2016.  PMID: 27818143.<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"http:\/\/www.m.u-tokyo.ac.jp\/news\/admin\/release_20161107.pdf\">\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u6d3b\u6027\u3092\u7c21\u4fbf\u304b\u3064\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3067\u304d\u308b\u65b0\u898f\u30d7\u30ed\u30fc\u30d6\u306e\u958b\u767a\uff08\u6771\u4eac\u5927\u5b66 2016\u5e7411\u670817 \u65e5\uff09<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14600\">pMRX-IP-GFP-LC3-RFP-LC3deltaG (catalog#RDB14600)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14601\">pMRX-IP-GFP-LC3-RFP (catalog#RDB14601)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0063j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u9ad8\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\uff06\u30a6\u30df\u30b5\u30dc\u30c6\u30f3\u7531\u6765GFP (2016\/12\/28 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u9ad8\u767a\u5149\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\n<ul style=\"line-height: 1.5em;\">\n<li>D-\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u306b\u3088\u308b\u9ad8\u3044\u5f37\u5ea6\u306e\u767a\u5149\u304c\u5f97\u3089\u308c\u307e\u3059<\/li>\n<li>\u9078\u3079\u308b 3 \u8272\u767a\u5149 \uff01\u591a\u8272\u89b3\u5bdf\u306b\u5fdc\u7528\u3067\u304d\u307e\u3059<\/li>\n<li>\u74b0\u5883\u8981\u56e0\u306b\u4f5c\u7528\u3055\u308c\u306b\u304f\u3044\u9ad8\u3044\u5b89\u5b9a\u6027\u3092\u8a87\u308a\u307e\u3059<\/li>\n<\/ul>\n<\/li>\n<li>\u30a6\u30df\u30b5\u30dc\u30c6\u30f3\u7531\u6765GFP\n<ul style=\"line-height: 1.5em;\">\n<li>\u8010\u71b1\u6027GFP<\/li>\n<li>pH\u611f\u53d7\u6027GFP<\/li>\n<\/ul>\n<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0025j\">\u4e00\u89a7\u306f\u3053\u3061\u3089<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><a name=\"EXR0062j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u751f\u304d\u305f\u52d5\u7269\u7d30\u80de\u3067\u306e\u8102\u8cea\u30e9\u30d5\u30c8\u306e\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3059\u308b\u7121\u6bd2\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u300c\u30ca\u30ab\u30ce\u30ea\u300d(2016\/08\/29 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7d30\u80de\u819c\u5fae\u5c0f\u9818\u57df\u306e\u4e00\u90e8\u3067\u3042\u308a\u3001\u30b9\u30d5\u30a3\u30f3\u30b4\u30df\u30a8\u30ea\u30f3\u3068\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u3092\u4e3b\u6210\u5206\u3068\u3059\u308b\u8102\u8cea\u30e9\u30d5\u30c8\u306f\u3001\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3084\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u306a\u3069\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u8102\u8cea\u30e9\u30d5\u30c8\u3092\u6a19\u8b58\u3059\u308b\u6e80\u8db3\u306e\u3044\u304f\u624b\u6cd5\u304c\u306a\u3044\u305f\u3081\u3001\u8a73\u7d30\u306a\u89e3\u6790\u304c\u56f0\u96e3\u3067\u3057\u305f\u3002\u7406\u5316\u5b66\u7814\u7a76\u6240\u3000\u5c0f\u6797\u8102\u8cea\u751f\u7269\u5b66\u7814\u7a76\u5ba4\u306e\u5c0f\u6797\u4fca\u79c0\u5148\u751f\uff08\u7814\u7a76\u5f53\u6642\uff09\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u4eba\u5de5\u7684\u306a\u8102\u8cea\u30e9\u30d5\u30c8\u3092\u4f5c\u88fd\u3057\u3001\u7d50\u5408\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u305f\u7d50\u679c\u3001\u30b9\u30d5\u30a3\u30f3\u30b4\u30df\u30a8\u30ea\u30f3\u3068\u30b3\u30ec\u30b9\u30c6\u30ed\u30fc\u30eb\u8907\u5408\u4f53\u306b\u306e\u307f\u306b\u7d50\u5408\u3059\u308b\u3001\u3059\u306a\u308f\u3061\u8102\u8cea\u30e9\u30d5\u30c8\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u30de\u30a4\u30bf\u30b1\u7531\u6765\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u300c\u30ca\u30ab\u30ce\u30ea\u300d\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u8102\u8cea\u30e9\u30d5\u30c8\u3092\u30ca\u30ab\u30ce\u30ea\u306b\u3088\u308a\u6a19\u8b58\u3057\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u67d3\u8272\u306b\u3088\u308a\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u3092\u691c\u51fa\u3057\u305f\u3068\u3053\u308d\u3001\u8102\u8cea\u30e9\u30d5\u30c8\u306e\u7e01\u304b\u3089\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u304c\u51fa\u82bd\u3059\u308b\u69d8\u304c\u89b3\u5bdf\u3055\u308c\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u30ca\u30ab\u30ce\u30ea\u306f\u7121\u6bd2\u6027\u3067\u3042\u308b\u305f\u3081\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u878d\u5408\u3057\u305f\u30ca\u30ab\u30ce\u30ea\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u751f\u304d\u305f\u52d5\u7269\u7d30\u80de\u3067\u306e\u8102\u8cea\u30e9\u30d5\u30c8\u306e\u6a19\u8b58\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u3092\u611f\u67d3\u3055\u305b\u305f\u57f9\u990a\u7d30\u80de\u306b\u30ca\u30ab\u30ce\u30ea\u3092\u52a0\u3048\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u6b96\u3092\u6291\u3048\u3089\u308c\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nMakino, A. et al. A novel sphingomyelin\/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB. J., 2016. Aug, 4. In press. PMID: 27492925.<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2016\/20160822_2\/\">\u30de\u30a4\u30bf\u30b1\u7531\u6765\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306e\u5897\u6b96\u3092\u6291\u5236\uff08\u7406\u5316\u5b66\u7814\u7a76\u6240 2016\u5e748\u670822\u65e5\uff09<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14300\">pET28\/His6-mCherry-D4 (catalog#RDB14300)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14301\">pET28\/His6-EGFP-Nakanori (catalog#RDB14301)<\/a>\n<\/li>\n<\/ul>\n<p><a name=\"EXR0059j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u8272\u3067\u5206\u5b50\u6df7\u96d1\u3092\u8a55\u4fa1\u3067\u304d\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u300cGimRET\u300d(2016\/07\/11 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7d30\u80de\u5185\u306f\u3001\u69d8\u3005\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u3063\u3066\u5145\u305f\u3055\u308c\u3066\u304a\u308a\u3001\u3053\u306e\u72b6\u614b\u3092\u5206\u5b50\u6df7\u96d1\u3068\u3044\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u5206\u5b50\u6df7\u96d1\u3092\u52b9\u679c\u7684\u306b\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u56f0\u96e3\u3067\u3057\u305f\u3002\u7406\u5316\u5b66\u7814\u7a76\u6240 \u751f\u547d\u30b7\u30b9\u30c6\u30e0\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u6e21\u9089\u670b\u4fe1\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u5206\u5b50\u6df7\u96d1\u3068\u7d30\u80de\u5185\u306e\u6c34\u5206\u5b50\u306e\u72b6\u614b\u306e\u95a2\u4fc2\u306b\u6ce8\u76ee\u3057\u3001\u6c34\u5206\u5b50\u306e\u72b6\u614b\u306b\u3088\u308a\u3001\u86cd\u5149\u5f37\u5ea6\u304c\u5909\u308f\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u86cd\u5149\u5171\u9cf4\u30a8\u30cd\u30eb\u30ae\u30fc\u79fb\u52d5\u6cd5\uff08FRET\u6cd5\uff09\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u5185\u306e\u5206\u5b50\u6df7\u96d1\u306e\u5ea6\u5408\u3044\u306b\u3088\u3063\u3066\u8272\u304c\u5909\u5316\u3059\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u300cGimRET\u300d\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nMorikawa, J.T., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, M.T. Sci. Rep. 6: 22342, 2016. PMID: 26956628<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"http:\/\/www.riken.jp\/pr\/press\/2016\/20160309_1\/\">\u5206\u5b50\u6df7\u96d1\u304c\u8a08\u6e2c\u3067\u304d\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u300cGimRET\u300d\u306e\u958b\u767a\uff08\u7406\u5316\u5b66\u7814\u7a76\u6240 2016\u5e743\u67089\u65e5\uff09<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14203\">pPAL7_GimRET (CFP-YFP1G\/pPAL7) (catalog#RDB14203)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14204\">pGimRET (CFP-YFP1G\/pECFP) (catalog#RDB14204)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0055j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u5358\u4e00\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u767a\u73fe\u53ef\u80fd\u306a\u7d30\u80de\u5468\u671f\u89b3\u5bdf\u7528\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaFucci2a (2016\/06\/17 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>Fucci2a\u306f\u30012\u7a2e\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea(mCherry \u304a\u3088\u3073mVenus)\u306b\u3001\u305d\u308c\u305e\u308cG1\u671f\u7279\u7570\u7684\u30bf\u30f3\u30d1\u30af\u8cea(Cdt1)\u304a\u3088\u3073S\/G2\/M\u671f\u7279\u7570\u7684\u30bf\u30f3\u30d1\u30af\u8cea(Geminin)\u306e\u7d30\u80de\u5468\u671f\u4f9d\u5b58\u7684\u306a\u30c7\u30b0\u30ed\u30f3\u914d\u5217\u3092\u878d\u5408\u3055\u305b\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002\u30d0\u30a4\u30b7\u30b9\u30c8\u30ed\u30cb\u30c3\u30af\u767a\u73fe\u7528\u914d\u5217 T2A\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u5358\u4e00\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30672\u7a2e\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u540c\u6642\u306b\u767a\u73fe\u3067\u304d\u307e\u3059\u3002Fucci2a\u3092\u642d\u8f09\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3068\u3001\u57f9\u990a\u7d30\u80de\u4e00\u3064\u4e00\u3064\u306e\u7d30\u80de\u5468\u671f\u306e\u72b6\u614b\u3092\u86cd\u5149\u9855\u5fae\u93e1\u3067\u691c\u77e5\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nMort, R.L., Ford, M.J., Sakaue-Sawano, A., Lindstrom, N.O., Casadio, A., Douglas, A.T., Keighren, M.A., Hohenstein, P., Miyawaki, A., Jackson, I.J. Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696, 2014.<\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9\n<ol style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13080\">pCAG-Fucci2a (catalog#RDB13080)<\/a> for transient expression of Fucci2a marker.<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13081\">pROSA-floxNeo-Fucci2a (catalog#RDB13081)<\/a> for targeted insertion of Fucci2a marker into mouse ROSA26 locus.<\/li>\n<\/ol>\n<p>\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9 B6;129-Gt(ROSA)26Sor&lt;tm1(Fucci2aR)Jkn&gt; (catalog # RBRC06511) \u306f\u5b9f\u9a13\u52d5\u7269\u958b\u767a\u5ba4\u304b\u3089\u63d0\u4f9b\u3044\u305f\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p><\/li>\n<\/ul>\n<p><a name=\"EXR0021j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> YidC\u306e\u7d50\u6676\u69cb\u9020\u89e3\u6790(2016\/5\/13 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>B. haloduran \u682a\u7531\u6765\u306e\u30b2\u30ce\u30e0DNA\u3092\u3082\u3068\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305fYidC\u907a\u4f1d\u5b50\u304b\u3089\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f5c\u88fd\u3057\u3001\u7d50\u6676\u69cb\u9020\u89e3\u6790\u306b\u5229\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKumazaki K, Tsukazaki T, Nishizawa T, Tanaka Y, Kato HE, Nakada-Nakura Y, Hirata K, Mori Y, Suga H, Dohmae N, Ishitani R, Nureki O. Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans. Acta Crystallogr F Struct Biol Commun. 70(Pt 8):1056-1060, 2014.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/JGD12232\">Genomic DNA of Bacillus halodurans, JCM9153 (Cat# JGD12232)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0037j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u82e5\u5e74\u6027\u30df\u30aa\u30af\u30ed\u30fc\u30cc\u30b9\u3066\u3093\u304b\u3093(JME) \u306b\u95a2\u4e0e\u3059\u308b\u9818\u57df( EJM1, 6p12-p11) \u306e\u89e3\u6790(2016\/4\/12 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7b46\u8005\u3089\u306f\u82e5\u5e74\u6027\u306e\u30df\u30aa\u30af\u30ed\u30fc\u30cc\u30b9\u3066\u3093\u304b\u3093 (JME) \u306b\u95a2\u4e0e\u3059\u308b\u30af\u30ed\u30e2\u30bd\u30fc\u30e0\u9818\u57df\u306e\uff11\u3064\u3068\u3057\u3066\u3001BF-HLA\u3068\u30ea\u30f3\u30af\u3059\u308b\uff16\u756a\u67d3\u8272\u4f53\u77ed\u8155\u4e0a6p12-p11\u306b\u30013.5 cM \u306e\u75be\u75c5\u539f\u56e0\u9818\u57df (EJM1) \u3092\u7d5e\u308a\u8fbc\u3093\u3067\u304d\u305f\u3002\u672c\u5831\u544a\u3067\u306f\u3001\u307e\u305a\u5f53\u8a72\u9818\u57df\u306e\u907a\u4f1d\u5b50\u30de\u30c3\u30d4\u30f3\u30b0\u3092\u884c\u3044\u3001\u305d\u306e\u30de\u30c3\u30d7\u3092\u57fa\u306b\u60a3\u8005\u3068\u5065\u5e38\u8005\u306e EJM1 \u9818\u57df\u4e2d\u306e14\u907a\u4f1d\u5b50\u306b\u3064\u3044\u3066\u30a8\u30af\u30bd\u30f3\u30a4\u30f3\u30c8\u30ed\u30f3\u89e3\u6790\u3092\u884c\u3044\u3001\u539f\u56e0\u907a\u4f1d\u5b50\u306e\u63a2\u7d22\u304c\u306a\u3055\u308c\u305f\u3002\u306a\u304a\u3001\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305f\u6570\u7a2e\u306eYAC\u30af\u30ed\u30fc\u30f3\u306f\u3001BAC\/PAC\u306e\u30a2\u30c3\u30bb\u30f3\u30d6\u30ea\u30b3\u30f3\u30c6\u30a3\u30b0\u306e\u9806\u5e8f\u3092\u6b63\u3057\u304f\u4e26\u3079\u308b\u305f\u3081\u306e\u53c2\u7167\u3068\u3057\u3066\u3001STS-PCR\u89e3\u6790\u306e\u92f3\u578b\u306b\u4f7f\u7528\u3055\u308c\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nSuzuki, T., Delgado-Escueta, A.V., Alonso, M.E., Morita, R., Okamura, N., Sugimoto, Y., Bai, D., Medina, M.T., Bailey, J.N., Rasmussen, A., Ramos-Peek, J., Cordova, S., Rubio-Donnadieu, F., Ochoa, A., Jara-Prado, A., Inazawa, J., Yamakawa, K. Mutation analyses of genes on 6p12-p11 in patients with juvenile myoclonic epilepsy. Neurosci. Lett. 405 (1-2): 126-131, 2006.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"\/ja\/cephyac_yac\">CEPH MEGA YAC clone<\/a><br \/>\n(771C3, 786E1, 834B12, 858B11, 918A11, 938C3, 961C3)<\/li>\n<\/ul>\n<p>&nbsp;<br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u7d30\u80de\u5185\u3067\u767a\u73fe\u3055\u305b\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4efb\u610f\u306b\u9664\u53bb (2016.03.25 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>Natsume, T., <i>et al<\/i>., 2016\u3067\u767a\u8868\u3057\u305f\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3\u6cd5 (AID\u6cd5) \u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9664\u53bb\u30b7\u30b9\u30c6\u30e0\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30af\u30ed\u30fc\u30f3\u304c\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nNatsume, T., Kiyomitsu, Y., Saga, Y., Kanemaki, M.T. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Reports 15: 210-218, 2016. PMID: 27052166.<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"https:\/\/www.nig.ac.jp\/nig\/ja\/2016\/03\/research-highlights_ja\/20160325.html\">\u53d6\u308a\u9664\u3051\u3070\u50cd\u304d\u304c\u308f\u304b\u308b\uff01\uff5e\u7279\u5b9a\u306e\u30d2\u30c8\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d20\u65e9\u304f\u53d6\u308a\u9664\u3044\u3066\u6a5f\u80fd\u3092\u63a2\u308b\u65b9\u6cd5\u3092\u958b\u767a\uff5e <\/a><br \/>\n<a href=\"https:\/\/www.nig.ac.jp\/nig\/images\/research_highlights\/PR20160325.pdf\">\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\u8cc7\u6599 [PDF]<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"\/ja\/gene_analysis\/rdb08468ja\">Figure 3\u306epMK232, PMK243\u3001AAVS1\u907a\u4f1d\u5b50\u5ea7\u3092\u6a19\u7684\u3068\u3059\u308b\u30ac\u30a4\u30c9RNA\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3068Figure S6a \u306e18\u30af\u30ed\u30fc\u30f3\u3002<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0045j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30d2\u30c8TRB3\u30b3\u30f3\u30c7\u30a3\u30b7\u30e7\u30ca\u30eb\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u3068TRB3\u306e\u7d44\u7e54\u7279\u7570\u7684\u306a\u5f62\u614b\u3078\u306e\u5f71\u97ff (2016\/3\/11 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7b46\u8005\u3089\u306e\u30de\u30a6\u30b9\u816b\u760d\u7d30\u80de\u3092\u7528\u3044\u305f\u306e\u7814\u7a76\u304b\u3089\u30d2\u30c8Tribbles related protein 3 (TRB3) pseudokinase \u306f\u816b\u760d\u7d30\u80de\u306e\u5897\u6b96\u306e\u4ea2\u9032\u3068\u6838\u306e\u5897\u5927\u306b\u5f71\u97ff\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u305f\u3002\u672c\u7814\u7a76\u3067\u306f\u30de\u30a6\u30b9\u6b63\u5e38\u809d\u7d44\u7e54\u3067\u306ede novo \u306e\u8868\u73fe\u578b\u3078\u306e\u5f71\u97ff\u3092\u63a2\u308b\u305f\u3081Cre \/ loxp \u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308a\u5c40\u6240\u7684\u306b\u30d2\u30c8TRB3 \u3092\u767a\u73fe\u3055\u305b\u809d\u7d44\u7e54\u3067\u306e\u6838\u306e\u5897\u5927\u3092\u78ba\u8a8d\u3057\u305f\u3002Cre \/ loxp \u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u305f\u30d2\u30c8TRB3 \u30b3\u30f3\u30c7\u30a3\u30b7\u30e7\u30ca\u30eb\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u306b\u306f\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305fpCALNL5 (RDB01862) \u3068pxCANCre (RDB01675) \u304c\u4f7f\u7528\u3055\u308c\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nSakai, Y., Fukamachi, K., Futakuchi, M., Miyoshi, I., Tsuda, H., Suzui, M., Hayashi, H. A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull. 37 (6): 1068-1074, 2014.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01862\">pCALNL5 (cat# RDB01862)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01675\">pxCANCre (cat# RDB01675)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0044j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u795e\u7d4c\u306eALS\u75c7\u306b\u95a2\u4e0e\u3059\u308bFUS\/TLS\u306e\u5909\u7570\u306fRNA\u9846\u7c92\u306e\u5f62\u6210\u80fd\u3092\u4f4e\u4e0b\u3059\u308b\u3002 (2016\/2\/5 M.O.)<\/p>\n<ul>\n<li>\u7b46\u8005\u3089\u306fFUS\/TLS\u306eC\u672b\u7aef\u306b\u3042\u308b\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u306e\u5909\u7570\u304c\u5f53\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u51dd\u96c6\u3092\u5f15\u304d\u8d77\u3053\u3059\u3053\u3068\u3001\u307e\u305f\u305d\u306e\u7d50\u679c\u3001\u7d30\u80de\u8ceaRNA\u9846\u7c92 (P-body) \u306e\u5f62\u6210\u304c\u7570\u5e38\u306b\u306a\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u305f\u3002\u672c\u7814\u7a76\u306b\u304a\u3044\u3066\u57f9\u990a\u7d30\u80de\u4e2d\u306eP-body\u3092\u53ef\u8996\u5316\u3059\u308b\u305f\u3081\u3001\u30de\u30fc\u30ab\u30fc\u3068\u306a\u308bGFP\u30bf\u30b0\u3092\u4ed8\u52a0\u3057\u305fDCP1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u69cb\u7bc9\u3059\u308b\u305f\u3081\u306b\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305fDCP1 \u907a\u4f1d\u5b50\u30af\u30ed\u30fc\u30f3(IRAL010J08\u3001cat# HGY084224) \u3092\u4f7f\u7528\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nTakanashi, K., Yamaguchi, A., Aggregation of ALS-linked FUS mutant sequesters RNA binding proteins and impairs RNA granules formation. Biochem. Biophys. Res. Commun. 452 (3): 600-607, 2014.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGY084224\">IRAL010J08 (cat# HGY084224)<\/a><\/li>\n<\/ul>\n<p>&nbsp;<br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/> \u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u3092\u5411\u4e0a\u3055\u305b\u305fCas9-poly(A)\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9 (2016.01.22 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>lsODN (\u9577\u9396\u4e00\u672c\u9396 DNA)\u6cd5 \u306a\u3089\u3073\u306b2H2OP (2\u30d2\u30c3\u30c82\u30aa\u30ea\u30b4)\u6cd5\u3068\u3068\u3082\u306bYoshimi, K. <i>et al<\/i>., 2016\u3067\u4f7f\u7528\u3055\u308c\u305f\u3001Cas9\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9 T7-NLS hCas9-pA (cat# RDB13130) \u304c\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nYoshimi, K., Kunihiro, Y., Kaneko, T., Nagahora, H., Voigt, B., Mashimo, T.<br \/>\nssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.<br \/>\nNat. Commun. 7: 10431, 2016.<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<br \/>\n<a href=\"http:\/\/resou.osaka-u.ac.jp\/ja\/research\/2016\/20160120_1\">\u30de\u30a6\u30b9\u30fb\u30e9\u30c3\u30c8\u7b49\u306e\u907a\u4f1d\u5b50\u6539\u5909\u52b9\u7387\u3092\u5411\u4e0a\u3055\u305b\u308b\u65b0\u3057\u3044\u6280\u8853\u3092\u958b\u767a<\/a><\/li>\n<li>\u5bc4\u8a17\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB13130\">T7-NLS hCas9-pA (cat# RDB13130)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0043j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u6d3b\u6027\u5316\u3057\u305f\u30d2\u30c8\u597d\u4e2d\u7403\u3067\u306e\u30a4\u30f3\u30bf\u30fc\u30ed\u30a4\u30ad\u30f36\u306e\u9069\u5207\u306a\u767a\u73fe\u306b\u306f\u30af\u30ed\u30de\u30c1\u30f3\u518d\u69cb\u6210\u3068TNF\u03b1\u306e\u81ea\u5df1\u5206\u6ccc\u304c\u5fc5\u8981\u3067\u3042\u308b (2015\/12\/18 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7b46\u8005\u3089\u306f\u30d2\u30c8\u597d\u4e2d\u7403\u3067\u306eIL6\u306e\u767a\u73fe\u306e\u8abf\u7bc0\u6a5f\u69cb\u3092\u8abf\u3079\u308b\u305f\u3081\u3001\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4\u6cd5\u3092\u7528\u3044\u3066IL6\u4e0a\u6d41\u306b\u3042\u308b\u8ee2\u5199\u8abf\u7bc0\u9818\u57df\u306e\u5f71\u97ff\u3092\u691c\u8a3c\u3057\u305f\u3002\u4f7f\u7528\u3057\u305f\u30ec\u30dd\u30fc\u30bf\u30fc\u30af\u30ed\u30fc\u30f3\u306f\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305fRDB07313 pGL4-phIL6\u3092\u3082\u3068\u306b\u69cb\u7bc9\u3055\u308c\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nZimmermann, M., Aguilera, F.B., Castellucci, M., Rossato, M., Costa, S., Lunardi, C., Ostuni, R., Girolomoni, G., Natoli, G., Bazzoni, F., Tamassia, N., Cassatella, M.A. Chromatin remodelling and autocrine TNF\u03b1 are required for optimal interleukin-6 expression in activated human neutrophils. Nat. Commun. 6: 6061, 2015.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07313\">pGL4-phIL6 (cat# RDB07313)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0030j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Wt1a\u3001Foxc1a\u304a\u3088\u3073Notch \u30e1\u30c7\u30a3\u30a8\u30fc\u30bf\u30fc\u3067\u3042\u308bRbpj\u306f\u76f8\u4e92\u4f5c\u7528\u3057\u3066\u7cf8\u7403\u4f53\u4e0a\u76ae\u7d30\u80de\u306e\u5f62\u6210\u3092\u5236\u5fa1\u3059\u308b (2015\/11\/12 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>Notch\u30b7\u30b0\u30ca\u30eb\u3068\u540c\u3058\u304fWt1a\u3001Foxc1a\u306f\u7cf8\u7403\u4f53\u4e0a\u76ae\u7d30\u80de\u306e\u767a\u751f\u904b\u547d\u3092\u8abf\u7bc0\u3059\u308b\u56e0\u5b50\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u7b46\u8005\u3089\u306f\u3053\u308c\u3089\u56e0\u5b50\u304c\u76f8\u4e92\u4f5c\u7528\u3057\u3001\u7cf8\u7403\u4f53\u4e0a\u76ae\u7d30\u80de\u7279\u7570\u7684\u306b\u50cd\u304f\u907a\u4f1d\u5b50\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u306a\u304a\u4eca\u56de\u884c\u308f\u308c\u305f\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306bpEF-BOSneo-mNotch1 RAMIC (RDB06771)\u3068pCMX-N\/RBP-J (RDB03021)\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4\u306b pGa981-6 (RDB06776)\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nO&#8217;Brien, L.L., Grimaldi, M., Kostun, Z., Wingert, R. A., Selleck, R., Davidson, A.J. Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol. 358 (2): 318-330, 2011.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03021\">pCMX-N\/RBP-J (cat# RDB03021)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06771\">pEF-BOSneo-mNotch1 RAMIC (cat# RDB06771)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06776\">pGa981-6 (cat# RDB06776)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0039j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Lin28a (RNA binding protein) \u306f\u3044\u304f\u3064\u304b\u306e\u5589\u982d\u304c\u3093\u5e79\u7d30\u80de\u3092\u4ea2\u9032\u3059\u308b\u56e0\u5b50\u3067\u3042\u308b(2015\/10\/23 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7b46\u8005\u3089\u306f\u3001\u9ad8\u3044\u5e79\u7d30\u80de\u6d3b\u6027\u3092\u6301\u3064\u7d30\u80de\u5206\u753b\u3067\u3042\u308bSP\u7d30\u80de\u3067\u306fLIN28\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u304c\u4ea2\u9032\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u305f\u3002LIN28\u907a\u4f1d\u5b50\u3068\u5e79\u7d30\u80de\u6d3b\u6027\u306e\u95a2\u4fc2\u3092\u8abf\u3079\u308b\u4e2d\u3067\u3001LIN28\u767a\u73fe\u30d9\u30af\u30bf\u30fc(RDB06602)\u3092\u5589\u982d\u304c\u3093\u7d30\u80de\u306b\u5c0e\u5165\u3057\u3001\u7d30\u80de\u5897\u6b96\u304c\u4fc3\u9032\u3055\u308c\u308b\u3053\u3068\u3001\u56fa\u76f8\u57f9\u5730\u3078\u306e\u6d78\u6f64\u80fd\u304c\u9ad8\u304f\u306a\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u305f\u3002\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u5b9f\u9a13\u306b\u306fRDB05956\u4f7f\u7528\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nHayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K. Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma. Exp. Cell Res. 319 (8): 1220-1228, 2013<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06602\">pCMFlag_hsLIN28 (cat# RDB06602)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05956\">pCMV_S-FLAG (cat# RDB05956)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0028j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> JNK\/AP-1\u306e\u30b7\u30b0\u30ca\u30eb\u306b\u3088\u308b\u8ee2\u5199\u6d3b\u6027\u306e\u6307\u6a19 (2015\/10\/6 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7b46\u8005\u3089\u306f\u3001\u30b1\u30e9\u30c1\u30ce\u30b5\u30a4\u30c8\u306e\u5206\u5316\u904e\u7a0b\u306b\u304a\u3051\u308bK1(\u30b1\u30e9\u30c1\u30f31) \u767a\u73fe\u304c\u5f8c\u671f\u306b\u7279\u7570\u7684\u306a\u539f\u56e0\u3092\u8abf\u3079\u308b\u305f\u3081\u3001\u8ee2\u5199\u5236\u5fa1\u6a5f\u69cb\u3092\u8abf\u3079\u305f\u3002K1\u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u5236\u5fa1\u9818\u57df\u306b\u306f\u3001p63\u306b\u4f34\u3046JNK\/AP-1\u30b7\u30b0\u30ca\u30eb\u306b\u3088\u3063\u3066\u6d3b\u6027\u5316\u3055\u308c\u308bAP-1\u8a8d\u8b58\u914d\u5217\u304c\u3042\u308b\u306e\u3067\u3001\u5f53\u8a72\u9818\u57df\u306e\u8ee2\u5199\u6d3b\u6027\u3092AP-1\u7279\u7570\u7684\u306a\u8ee2\u5199\u6d3b\u6027\u3068\u6bd4\u8f03\u3057\u3001\u691c\u8a0e\u3057\u305f\u3002\u306a\u304a\u3001AP-1\u7279\u7570\u7684\u306a\u8ee2\u5199\u6d3b\u6027\u306e\u6e2c\u5b9a\u306b\u306f\u3001\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305fp3AP1(PMA)RE-TK hRluc(F)\u304c\u4f7f\u7528\u3055\u308c\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nOgawa E, Okuyama R, Egawa T, Nagoshi H, Obinata M, Tagami H, Ikawa S, Aiba. p63\/p51-induced onset of keratinocyte differentiation via the c-Jun N-terminal kinase pathway is counteracted by keratinocyte growth factor. J Biol Chem. 283(49):34241-9, 2008<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03452\">p3AP1 (PMA) RE-TK hRluc(F) (cat# RDB03452)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0084j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>Histac \uff5e\u30d2\u30b9\u30c8\u30f3\u306e\u30a2\u30bb\u30c1\u30eb\u5316\u3092\u691c\u51fa\u3059\u308b\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\uff5e (2015\/9\/15 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30d2\u30b9\u30c8\u30f3\u4fee\u98fe\u306f\u3001\u907a\u4f1d\u5b50\u306e\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u306a\u767a\u73fe\u5236\u5fa1\u306b\u5927\u304d\u304f\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3044\u3064\u30fb\u3069\u3053\u3067\u30fb\u3069\u306e\u3088\u3046\u306b\u8d77\u304d\u308b\u306e\u304b\u3068\u3044\u3046\u52d5\u614b\u89e3\u6790\u306f\u307b\u3068\u3093\u3069\u9032\u3093\u3067\u3044\u307e\u305b\u3093\u3002\u751f\u304d\u305f\u307e\u307e\u89e3\u6790\u3059\u308b\u624b\u6bb5\u304c\u4e4f\u3057\u3044\u3053\u3068\u304c\u7406\u7531\u306e\u3072\u3068\u3064\u3068\u3057\u3066\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/li>\n<li>\u4eca\u56de\u306f\u3001\u4f50\u3005\u6728 \u548c\u6a39\u5148\u751f\uff08\u7406\u5316\u5b66\u7814\u7a76\u6240\u30fb\u8133\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff09\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304c\u958b\u767a\u3057\u305f\u300cHistac\u300d\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002\u300cHistac\u300d\u306f\u3001\u30d2\u30b9\u30c8\u30f3H4\u306e\u30a2\u30bb\u30c1\u30eb\u5316\u306e\u52d5\u614b\u3092\u751f\u7d30\u80de\u3067\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u89e3\u6790\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u305f\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u3067\u3059\u3002<\/li>\n<li>Histac\u306e\u7279\u5fb4\n<ul style=\"line-height: 1.5em;\">\n<li>Histac\u306f\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaVenus\u3001\u30a2\u30bb\u30c1\u30eb\u5316\u30d2\u30b9\u30c8\u30f3\u3068\u7d50\u5408\u3059\u308b\u30d6\u30ed\u30e2\u30c9\u30e1\u30a4\u30f3\u3001\u30d2\u30b9\u30c8\u30f3H4\u3001\u304a\u3088\u3073\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaCFP\u3092\u76f4\u5217\u306b\u3064\u306a\u3044\u3060\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002Histac\u5185\u306eH4\u306e\u30a2\u30bb\u30c1\u30eb\u5316\u524d\u306b\u306f\u3001CFP\u3068Venus\u306e\u8ddd\u96e2\u304c\u8fd1\u304f\u306a\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u3066\u304a\u308a\u3001\u86cd\u5149\u5171\u9cf4\u30a8\u30cd\u30eb\u30ae\u30fc\u79fb\u52d5\uff08FRET\uff09\u306b\u3088\u308b\u9ec4\u8272\u86cd\u5149\uff08535 nm\uff09\u3092\u767a\u8272\u3057\u307e\u3059\u3002\u4e00\u65b9\u3001Histac\u5185\u306eH4\u306e\u30a2\u30bb\u30c1\u30eb\u5316\u306b\u3088\u308a\u3001\u30d6\u30ed\u30e2\u30c9\u30e1\u30a4\u30f3\u304cH4\u306b\u5206\u5b50\u5185\u4f1a\u5408\u3057\u3001CFP\u3068Venus\u306e\u8ddd\u96e2\u304c\u9060\u304f\u306a\u308b\u3053\u3068\u3067\u9752\u8272\u86cd\u5149\uff08480 nm\uff09\u3092\u767a\u8272\u3057\u307e\u3059\u3002<\/li>\n<li>Histac\u3092\u7528\u3044\u305f\u86cd\u5149\u89b3\u5bdf\u306b\u306f\u3001\u6297\u4f53\u53cd\u5fdc\u3084\u7d30\u80de\u56fa\u5b9a\u306f\u4e0d\u8981\u3067\u3059\u3002Histac\u3092\u5c0e\u5165\u3057\u305f\u52d5\u7269\u7d30\u80de\u3067\u306f\u3001\u86cd\u5149\u9855\u5fae\u93e1\u3092\u7528\u3044\u3066\u30a2\u30bb\u30c1\u30eb\u5316\u306e\u52d5\u614b\u3092\u7d4c\u6642\u7684\u306b\u8ffd\u8de1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u539f\u8457\u8ad6\u6587\u3067\u306f\u3001Histac\u767a\u73fe\u7d30\u80de\u682a\u306b\u8131\u30a2\u30bb\u30c1\u30eb\u5316\u9175\u7d20\u963b\u5bb3\u5264\u3067\u3042\u308bTSA\u3092\u4f5c\u7528\u3055\u305b\u3001\u30d2\u30b9\u30c8\u30f3H4\u306e\u30a2\u30bb\u30c1\u30eb\u5316\u306e\u5fdc\u7b54\u30920\u5206\u304b\u3089180\u5206\u307e\u3067\u3092\u7d4c\u6642\u7684\u306b\u89b3\u5bdf\u3057\u305f\u7d50\u679c\u3001\u6838\u5185\u306eCFP\u3068Venus\u306e\u86cd\u5149\u5f37\u5ea6\u6bd4\uff08480 nm\/535 nm\uff09\u304c\u5909\u5316\u3059\u308b\u3053\u3068\u304b\u3089\u3001\u30a2\u30bb\u30c1\u30eb\u5316\u306e\u52d5\u614b\u3092\u53ef\u8996\u5316\u8ffd\u8de1\u3067\u304d\u308b\u7814\u7a76\u30c4\u30fc\u30eb\u3068\u3057\u3066\u300cHistac\u300d\u306e\u6709\u52b9\u6027\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u539f\u8457\u8ad6\u6587\n<ul style=\"line-height: 1.5em;\">\n<li>Sasaki, K., et al. Real-time imaging of histone H4 hyperacetylation in living cells. PNAS, 106(38):16257-62, 2009.<\/li>\n<li>Ito T, et al. Real-Time Imaging of Histone H4K12-Specific Acetylation Determines the Modes of Action of Histone Deacetylase and Bromodomain Inhibitors. Chemistry &#038; Biology, 18(4):495-507, 2011.<\/li>\n<\/ul>\n<\/li>\n<li>\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"http:\/\/www.riken.jp\/pr\/press\/2009\/20090907\/\">\u907a\u4f1d\u5b50\u306e\u50cd\u304d\u3092\u6c7a\u3081\u308b\u30d2\u30b9\u30c8\u30f3\u30a2\u30bb\u30c1\u30eb\u5316\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u89b3\u5bdf<\/a><\/li>\n<li><a href=\"http:\/\/www.riken.jp\/outreach\/ip\/21193\">\u30d2\u30b9\u30c8\u30f3\u30a2\u30bb\u30c1\u30eb\u5316\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u86cd\u5149\u30d7\u30ed\u30fc\u30d6<\/a><\/li>\n<\/ul>\n<\/li>\n<li>\u30ea\u30bd\u30fc\u30b9\u306b\u3064\u3044\u3066\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12840\">Histac pcDNA3.1<\/a> (cat# RDB12840)<br \/>\nBRDT\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d6\u30ed\u30e2\u30c9\u30e1\u30a4\u30f3\u306b\u3088\u308a\u3001Histac\u5185\u306e\u30d2\u30b9\u30c8\u30f3H4\u306e5\u756a\u76ee\u304a\u3088\u30738\u756a\u76ee\u306e\u30ea\u30b8\u30f3\u6b8b\u57fa\u306e\u30a2\u30bb\u30c1\u30eb\u5316\uff08H4K5\/K8\uff09\u3092\u691c\u51fa\u3057\u307e\u3059\u3002<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB12841\">Histac-K12 pcDNA3.1<\/a> (cat# RDB12841)<br \/>\nBRD2\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d6\u30ed\u30e2\u30c9\u30e1\u30a4\u30f3\u306b\u3088\u308aHistac\u5185\u306e\u30d2\u30b9\u30c8\u30f3H4\u306e12\u756a\u76ee\u306e\u30ea\u30b8\u30f3\u6b8b\u57fa\u306e\u30a2\u30bb\u30c1\u30eb\u5316\uff08H4\/K12\uff09\u3092\u691c\u51fa\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><a name=\"EXR0085j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/>\u30b0\u30ea\u30aa\u30d6\u30e9\u30b9\u30c8\u30fc\u30de\u306e\u6d78\u6f64\u306b\u304a\u3044\u3066PRRX1\u306fNotch\u30b7\u30b0\u30ca\u30eb\u3092\u6d3b\u6027\u5316\u3059\u308b (2015\/9\/15 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30b0\u30ea\u30aa\u30d6\u30e9\u30b9\u30c8\u30fc\u30de\u306e\u6d78\u6f64\u306b\u306fPRRX1\u304c\u95a2\u308f\u3063\u3066\u304a\u308a\u3001\u307e\u305fNotch\u3001Wnt\u3001Hedgehog\u306e\u30d1\u30b9\u30a6\u30a7\u30a4\u304c\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u5343\u8cc0 \u5a01 \u5148\u751f\uff08\u540d\u53e4\u5c4b\u5927\u5b66\u5927\u5b66\u9662\u30fb\u533b\u5b66\u7814\u7a76\u79d1\uff09\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306fPRRX1A\u306e\u767a\u73fe\u306b\u3088\u3063\u3066\u3053\u308c\u3089\u30d1\u30b9\u30a6\u30a7\u30a4\u304c\u6d3b\u6027\u5316\u3055\u308c\u308b\u304b\u691c\u8a3c\u3059\u308b\u305f\u3081\u306b\u3001Notch\u30b7\u30b0\u30ca\u30eb\u306fpGa981-6 (cat. #RDB06776)\u3001Hedgehog\u30b7\u30b0\u30ca\u30eb\u306b\u3064\u3044\u3066\u306f8&#215;3&#8217;Gli-BS-delta51-LucII (cat. #RDB08061)\u3092\u7528\u3044\u3066\u3001\u30ec\u30dd\u30fc\u30bf\u30fc\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/li>\n<li>\u539f\u8457\u8ad6\u6587\n<ul style=\"line-height: 1.5em;\">\n<li>Sugiyama, M., et al. Paired related homeobox 1 is associated with the invasive properties of glioblastoma cells. Oncol Rep. 33(3):1123-30, 2015.<\/li>\n<\/ul>\n<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06776\">pGa981-6 (cat# RDB06776)<\/a><\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08061\">8&#215;3&#8217;Gli-BS-delta51-LucII  (cat# RDB08061)<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><a name=\"EXR0025j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30b0\u30eb\u30b3\u30b7\u30c0\u30fc\u30bcII\u306e\u57fa\u8cea\u7279\u7570\u6027\u306e\u89e3\u660e\u3002(2015\/9\/4 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30b0\u30eb\u30b3\u30b7\u30c0\u30fc\u30bcII\u306e\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u5404\u7a2e\u30b0\u30eb\u30b3\u30b7\u30eb\u30de\u30f3\u30ce\u30fc\u30b9\u306b\u5bfe\u3059\u308b\u57fa\u8cea\u7279\u7570\u6027\u306b\u3064\u3044\u3066\u8abf\u3079\u308b\u305f\u3081\u3001\u90e8\u4f4d\u7279\u7570\u7684\u5909\u7570\u306e\u92f3\u578b\u3068\u3057\u3066SpEnt26D11\u304c\u7528\u3044\u3089\u308c\u307e\u3057\u305f\u3002\u91ce\u751f\u578b\u53ca\u3073\u4f5c\u88fd\u3057\u305f\u5909\u7570\u907a\u4f1d\u5b50\u306e\u9175\u6bcd\u3078\u306e\u5c0e\u5165\u306bpREP1-ccdb2 (RDB06519) \u53ca\u3073\u3001\u3000pDUAL-YFH1c (RDB06151 )\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nStigliano ID, Caramelo JJ, Labriola CA, Parodi AJ, D&#8217;Alessio C. Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol Biol Cell. 20 (17): 3974-3984, 2009<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06519\">pREP1-ccdb2 (cat# RDB06519)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06151\">pDUAL-YFH1c (cat# RDB06151)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/SPW010483\">S.pombe entry, SpEnt26D11 (cat# SPW010483)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0024j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> ABCC1\u3078\u306e\u5909\u7570\u5c0e\u5165\u306b\u3088\u308b\u4e0a\u76ae\u7d30\u80de\u5074\u5e95\u819c\u3078\u306e\u5c40\u5728\u5909\u5316\u89b3\u5bdf\u3002(2015\/8\/17 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>ABCC1\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u4e0a\u76ae\u7d30\u80de\u5074\u5e95\u819c\u306b\u5c40\u5728\u3059\u308b\u306e\u306b\u5fc5\u8981\u306a\u8981\u56e0\u3092\u91ce\u751f\u578b\u30bf\u30f3\u30d1\u30af\u8cea\u3068di-leucine motif\u5909\u7570\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u89e3\u6790\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nEmi Y, Harada Y, Sakaguchi M. Involvement of a di-leucine motif in targeting of ABCC1 to the basolateral plasma membrane of polarized epithelial cells. Biochem. Biophys. Res. Commun. 441 (1): 89-95, 2013.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HKR324434\">ABCC1 cDNA clone, RBb11B10 (cat# HKR324434)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0042j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u8840\u6e05\u306e\u523a\u6fc0\u306b\u3088\u308b\u9593\u8449\u7cfb\u5e79\u7d30\u80de\u306e\u30aa\u30ea\u30b4\u30c7\u30f3\u30c9\u30ed\u30b5\u30a4\u30c8\u3078\u306e\u5206\u5316\u306fNotch1\u306e\u6291\u5236\u306b\u3088\u308a\u4ea2\u9032\u3055\u308c\u308b(2015\/7\/31 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7b46\u8005\u3089\u306f\u9593\u8449\u7cfb\u5e79\u7d30\u80de (KP-hMSCs) \u306e\u30aa\u30ea\u30b4\u30c7\u30f3\u30c9\u30ed\u30b5\u30a4\u30c8\u3078\u306e\u5206\u5316\u306b\u304a\u3051\u308bNotch \u30b7\u30b0\u30ca\u30eb\u30d1\u30b9\u30a6\u30a7\u30a4\u306e\u5f79\u5272\u3092\u8abf\u3079\u308b\u305f\u3081\u3001Notch1\u306e\u6a5f\u80fd\u3092\u963b\u5bb3\u3059\u308b\u30c9\u30df\u30ca\u30f3\u30c8\u30cd\u30ac\u30c6\u30a3\u30d6RBP-J (R218H)\u3092\u9593\u8449\u7cfb\u5e79\u7d30\u80de\u306b\u5c0e\u5165\u3057\u3001RBP-J (WT)\u3092\u5c0e\u5165\u3057\u305f\u5834\u5408\u3068\u6bd4\u8f03\u3057\u3066\u3001\u8840\u6e05\u306e\u523a\u6fc0\u306b\u3088\u308b\u30aa\u30ea\u30b4\u30c7\u30f3\u30c9\u30ed\u30b5\u30a4\u30c8\u30de\u30fc\u30ab\u30fc\u306e\u767a\u73fe\u91cf\u306e\u5909\u5316\u3092\u8abf\u3079\u305f\u3002\u3053\u306e\u5b9f\u9a13\u306b\u306f\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305fRDB03021 pCMX-N\/RBP-J\u3068RDB03022 pCMX-N\/RBP-J (R218H)\u304c\u4f7f\u7528\u3055\u308c\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nLee YJ, Hung SC, Chu MS. Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells Cloning 3:165-173, 2010<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03021\">pCMX-N\/RBP-J (cat# RDB03021)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03022\">pCMX-N\/RBP-J (R218H) (cat# RDB03022)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0038j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Sir3\u306ewinged helix \u30c9\u30e1\u30a4\u30f3\u3092\u4ecb\u3057\u305f\u4e8c\u91cf\u4f53\u5f62\u6210\u306f\u9175\u6bcd\u3067\u306e\u30d8\u30c6\u30ed\u30af\u30ed\u30de\u30c1\u30f3\u306e\u5f62\u6210\u306b\u5fc5\u9808\u3067\u3042\u308b (2015\/7\/17 Y.K.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u51fa\u82bd\u9175\u6bcd\u306e\u907a\u4f1d\u5b50\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u306b\u95a2\u4e0e\u3059\u308bSir3\u306eC\u672b\u7aef\u306b\u306f\u9032\u5316\u5b66\u7684\u306b\u4fdd\u5b58\u3055\u308c\u305fwH domain (winged helix-turn-helix domain) \u304c\u3042\u308b\u3002\u7b46\u8005\u3089\u306fwH domain\u306b\u3064\u3044\u3066\u305d\u306e\u69cb\u9020\u3068\u6a5f\u80fd\u3092\u89e3\u6790\u3057\u305f\u3002\u5f53\u5ba4\u304b\u3089\u63d0\u4f9b\u3057\u305fRDB07566 IRAK013J04 \u306e\u30d2\u30c8Orc1 wH \u30c9\u30e1\u30a4\u30f3\u306f\u5927\u8178\u83cc\u7528\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u7d44\u8fbc\u307e\u308c\u3001\u51fa\u82bd\u9175\u6bcd\u306ewH \u30c9\u30e1\u30a4\u30f3\u3068\u306e\u6bd4\u8f03\u306b\u4f7f\u7528\u3055\u308c\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nOppikofer M, Kueng S, Keusch JJ, Hassler M, Ladurner AG, Gut H, Gasser SM. Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation. EMBO J. 32 (3): 437-449, 2013<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/HGX005420\">IRAK013J04 : ORC1L (cat# HGX005420)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0035j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> WDR81\u907a\u4f1d\u5b50\u306f\u30d7\u30eb\u30ad\u30f3\u30a8\u7d30\u80de\u304a\u3088\u3073\u5149\u53d7\u5bb9\u7d30\u80de\u306e\u751f\u5b58\u306b\u5fc5\u8981\u3067\u3042\u308b (2015\/7\/4 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>ENU\u30df\u30e5\u30fc\u30bf\u30b8\u30a7\u30cd\u30b7\u30b9\u3067\u4f5c\u6210\u3055\u308c\u305f\u5909\u7570\u30de\u30a6\u30b9nur5\u306f\u3001\u30d7\u30eb\u30ad\u30f3\u30a8\u7d30\u80de\u5909\u6027\u3084\u5149\u53d7\u5bb9\u7d30\u80de\u306e\u6b20\u640d\u306b\u52a0\u3048\u3001\u9707\u3048\u3001\u4e26\u3073\u306b\u7570\u5e38\u306a\u6b69\u884c\u3092\u793a\u3057\u307e\u3059\u3002\u7b46\u8005\u3089\u306f\u3001\u91ce\u751f\u578b\u306eWDR81\u907a\u4f1d\u5b50\u3092\u542b\u3080BAC\u30af\u30ed\u30fc\u30f3(MSMg01-261K04)\u3092\u4f7f\u7528\u3057\u305f\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u306b\u3088\u3063\u3066\u8868\u73fe\u578b\u304c\u30ec\u30b9\u30ad\u30e5\u30fc\u3055\u308c\u308b\u3053\u3068\u304b\u3089\u3001WDR81\u907a\u4f1d\u5b50\u304cnur5\u30de\u30a6\u30b9\u306e\u539f\u56e0\u907a\u4f1d\u5b50\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nTraka M, Millen KJ, Collins D, Elbaz B, Kidd GJ, Gomez CM, Popko B. WDR81 is necessary for purkinje and photoreceptor cell survival. J Neurosci. 33 (16): 6834-6844, 2013.<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"\/ja\/clonesetja\/msmbac\">MSM Mouse BAC clone<\/a><br \/>\n<a href=\"http:\/\/analysis2.lab.nig.ac.jp\/mouseBrowser\/cgi-bin\/halDetail.cgi?org=mm&amp;chr=11&amp;level=7&amp;center=75280000&amp;keyword=MSMg01-261K04\">MSMg01-261K04 (\u30af\u30ed\u30fc\u30f3\u691c\u7d22\u7d50\u679c)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0034j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u8868\u9762\u30d7\u30e9\u30ba\u30e2\u30f3\u5171\u9cf4\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306b\u3088\u308b\u30ac\u30f3\u7d30\u80de\u3067\u306eEGFR\u30b7\u30b0\u30ca\u30eb\u306e\u691c\u51fa (2015\/6\/19 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u8868\u9762\u30d7\u30e9\u30ba\u30e2\u30f3\u5171\u9cf4(SPR)\u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u306f\u7d30\u80de\u5185\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3092\u5171\u9cf4\u89d2\u306e\u5909\u5316\u3068\u3057\u3066\u691c\u51fa\u3057\u307e\u3059\u3002\u7b46\u8005\u3089\u306fEGFR\u306e\u6d3b\u6027\u5316\u306b\u3068\u3082\u306a\u3046\u5171\u9cf4\u89d2\u306e\u5909\u5316\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u8abf\u3079\u308b\u305f\u3081\u3001\u91ce\u751f\u578bEGFR\u3068\u5909\u7570\u578bEGFR(kinase-dead)\u3092\u57f9\u990a\u7d30\u80de\u3067\u767a\u73fe\u3055\u305b\u89e3\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9\u306b\u306f\u5f53\u5ba4\u3067\u63d0\u4f9b\u3057\u305fpco12 EGFR (RDB01276)\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nHiragun T, Yanase Y, Kose K, Kawaguchi T, Uchida K, Tanaka S, Hide M. Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. Biosens Bioelectron. 32 (1) : 202 &#8211; 207 (2012).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01276\">pco12 EGFR (cat# RDB01276)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0020j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u9175\u6bcdS. pombe \u6838\u5c40\u5728\u30de\u30fc\u30ab\u30fc (2015\/6\/05 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>S. pombe \u306e\u6838\u5c0f\u4f53\u306b\u5c40\u5728\u3059\u308b\u30ea\u30dc\u30bd\u30fc\u30e0\u751f\u5408\u6210\u30bf\u30f3\u30d1\u30af\u8ceaRrp14-C\u306e\u30af\u30ed\u30fc\u30f3\u3067\u3059\u3002\u5bc4\u8a17\u3055\u308c\u305f\u30af\u30ed\u30fc\u30f3\u306fYFP\u30bf\u30b0\u4ed8\u304d\u3067\u3059\u304c\u3001CFP\u30bf\u30b0\u306b\u4ed8\u3051\u76f4\u3057\u3066\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u6838-\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d30\u80de\u5185\u8ddd\u96e2\u306e\u6e2c\u5b9a\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nJakociunas T, Domange Jordo M, Ait Mebarek M, Bunner CM, Verhein-Hansen J, Oddershede LB, Thon G. Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat. Proc Natl Acad Sci U S A. 110 (47): E4465-73 (2013).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/SPW083251\">SpYFH08C03 : orthlog of RRP14(Sc), SURF6(Hs) (cat# SPW083251)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0019j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30d5\u30a7\u30cb\u30eb\u30a2\u30e9\u30cb\u30f3\u5408\u6210\u7d4c\u8def\u306e\u9032\u5316\u5b66\u3078\u306e\u6d1e\u5bdf (2015\/5\/21 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30d0\u30af\u30c6\u30ea\u30a2\u304b\u3089\u690d\u7269\u3078\u306e\u30d5\u30a7\u30cb\u30eb\u30a2\u30e9\u30cb\u30f3\u5408\u6210\u7d4c\u8def\u306e\u9032\u5316\u3092\u751f\u5316\u5b66\u7684\u306b\u89e3\u660e\u3059\u308b\u305f\u3081\u69d8\u3005\u306a\u751f\u7269\u306e\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u9178-\u30d7\u30ec\u30d5\u30a7\u30f3\u9178\u8ee2\u79fb\u9175\u7d20\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3001\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7523\u751f\u3057\u307e\u3057\u305f\u3002\u4e00\u9023\u306e\u89e3\u6790\u3067Thermus thermophiles\u306e\u30b2\u30ce\u30e0\u3082\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nDornfeld C, Weisberg AJ, K C R, Dudareva N, Jelesko JG, Maeda HA. Phylobiochemical characterization of class-Ib aspartate\/prephenate aminotransferases reveals evolution of the plant arogenate phenylalanine pathway. Plant Cell 26 (7): 3101-3114 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/JGD05989\">Genomic DNA of Thermus thermophiles JCM 10941T (cat# JGD05989)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0017j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u75be\u75c5\u306e\u907a\u4f1d\u5b50\u6cbb\u7642\u306b\u5411\u3051\u305f\u30db\u30e4\u306eNADH dehydrogenase\u306e\u30af\u30ed\u30fc\u30f3\u4f5c\u88fd (2015\/5\/8 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>EST\u30af\u30ed\u30fc\u30f33\u682a\u304b\u3089\u69cb\u6210\u3057\u305f\u30db\u30e4\u306eNADH dehydrogenase\u5168\u9577\u914d\u5217\u3092\u7528\u3044\u3001\u57f9\u990a\u7d30\u80de\u767a\u73fe\u7528\u306a\u3089\u3073\u306b\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u500b\u4f53\u767a\u73fe\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f5c\u88fd\u3057\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u75c5\u30e2\u30c7\u30eb\u3068\u3057\u3066\u4f7f\u7528\u3055\u308c\u308b\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u5909\u7570\u4f53\u3067\u6a5f\u80fd\u3059\u308b\u304b\u89e3\u6790\u3055\u308c\u307e\u3057\u305f<\/li>\n<li>\u8ad6\u6587<br \/>\nGospodaryov DV, Lushchak OV, Rovenko BM, Perkhulyn NV, Gerards M, Tuomela T, Jacobs HT. Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila. Biochim Biophys Acta. 1837 (11): 1861-1869 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/dnaconda.riken.jp\/search\/ciona\/ciad062k07.html\">Ciona intestinalis EST Clones (ciad062k07)<\/a><br \/>\n<a href=\"https:\/\/dnaconda.riken.jp\/search\/ciona\/cibd016c12.html\">Ciona intestinalis EST Clones (cibd016c12)<\/a><br \/>\n<a href=\"https:\/\/dnaconda.riken.jp\/search\/ciona\/ciem809l11.html\">Ciona intestinalis EST Clones (ciem809l11)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0016j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> c-kit\u30ad\u30e1\u30e9\u53d7\u5bb9\u4f53\u3092\u7528\u3044\u305f\u4eba\u70ba\u7684\u306a\u7d30\u80de\u5897\u6b96\u306e\u5236\u5fa1 (2015\/4\/17 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u57f9\u990a\u6db2\u4e2d\u306b\u6297\u539f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u57f9\u990a\u7d30\u80de\u306e\u5897\u6b96\u3092\u4fc3\u9032\u3059\u308b\u6280\u8853\u3092\u958b\u767a\u3059\u308b\u305f\u3081\u306bc-kit\u306e\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u7d50\u5408\u90e8\u4f4d\u3092\u6297\u539f\u7d50\u5408\u90e8\u4f4d\u306b\u7f6e\u63db\u3057\u305f\u30ad\u30e1\u30e9\u53d7\u5bb9\u4f53\u3092\u4f5c\u88fd\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKaneko E, Kawahara M, Ueda H, Nagamune T. Growth control of genetically modified cells using an antibody\/c-Kit chimera. J Biosci Bioeng. 113 (5): 641-6 (2012).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01344\">pUC kit EH1 (cat# RDB01344)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0015j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Keratin 5 (K5)-Gsdma \u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u306e\u4f5c\u88fd (2015\/4\/3 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u4e0a\u76ae\u306e\u7dad\u6301\u3001\u6052\u5e38\u6027\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50GSDMA3 (wt\u3001A339T\u5909\u7570\u4f53)\u3092\u76ae\u819a\u3067\u904e\u5270\u767a\u73fe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u4f5c\u88fd\u7528\u30d9\u30af\u30bf\u30fc\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3057\u3066human K5 \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nTanaka S, Mizushina Y, Kato Y, Tamura M, Shiroishi T. Functional conservation of Gsdma cluster genes specifically duplicated in the mouse genome. G3 (Bethesda). 3 (10): 1843-50 (2013).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05886\">pKM2L-phK5 (cat# RDB05886)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0083j\"><\/a><br \/>\n<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" alt=\"\" \/>AID system (2015\/3\/18 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u3001\u85ac\u5264\u306b\u3088\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u3084\u907a\u4f1d\u5b50\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30fb\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u306f\u3001\u5e83\u304f\u5b9f\u65bd\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\u306e\u9418\u5dfb \u5c06\u4eba\u5148\u751f\u306e\u958b\u767a\u3057\u305f\u65b0\u305f\u306a\u5236\u5fa1\u65b9\u6cd5\u300cAuxin Inducible Degron\uff08AID\uff09system\u300d\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/li>\n<li>AID system\u306e\u7279\u5fb4\u306f\u3001\u690d\u7269\u30db\u30eb\u30e2\u30f3\u300c\u30aa\u30fc\u30ad\u30b7\u30f3\u300d\u306e\u6dfb\u52a0\u3092\u5f15\u304d\u91d1\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u3067\u3059\u3002<\/li>\n<li>\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u3088\u308aDegron\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u3092\u8a98\u767a\u3059\u308b\u9818\u57df\uff09\u3068\u878d\u5408\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3055\u305b\u3066\u304a\u304d\u3001\u30aa\u30fc\u30ad\u30b7\u30f3\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u308f\u305a\u304b15 &#8211; 30\u5206\u3067\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u306e\u767a\u73fe\u91cf\u306e\u5909\u5316\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u958b\u767a\u8005\u3067\u3042\u308b\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\u306e\u9418\u5dfb \u5c06\u4eba\u5148\u751f\u306b\u3088\u308b\u958b\u767a\u79d8\u8a71\u3084\u8a73\u3057\u3044\u8aac\u660e\u306f\u3001<a href=\"http:\/\/www.nig.ac.jp\/section\/k-interview\/kanemaki.html\">\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\u3000\u6559\u54e1\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc<\/a>\u304b\u3089\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u63d0\u4f9b\u30ea\u30bd\u30fc\u30b9\u306b\u3064\u3044\u3066\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08468\">pNHK60<\/a> (cat# RDB08468)<br \/>\nAID\u3068\u878d\u5408\u3057\u305fGFP\u3092\u767a\u73fe\u3057\u307e\u3059\u3002\u539f\u8457\u8ad6\u6587(1)\u3067\u306f\u30aa\u30fc\u30ad\u30b7\u30f3\u6dfb\u52a0\u306b\u3088\u308bGFP\u86cd\u5149\u306e\u6e1b\u8870\u3092\u793a\u5a01\u3059\u308b\u305f\u3081\u306b\u4f7f\u3063\u3066\u3044\u307e\u3059\u3002<\/li>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08469\">pMK106<\/a> (cat# RDB08469)<br \/>\nN\u672b\u7aef\u5074\u306bAID\u3092\u878d\u5408\u3057\u305f\u67d3\u8272\u4f53\u5236\u5fa1\u56e0\u5b50CENP-H\u3092\u767a\u73fe\u3057\u307e\u3059\u3002<\/p>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08470\">pMK107<\/a> (cat# RDB08470)<br \/>\nC\u672b\u7aef\u5074\u306bAID\u3092\u878d\u5408\u3057\u305fCENP-H\u3092\u767a\u73fe\u3057\u307e\u3059\u3002<\/p>\n<li>\u539f\u8457\u8ad6\u6587\u3067\u306f\u3001\u30cb\u30ef\u30c8\u30ea\u7531\u6765\u306eDT40\u7d30\u80de\u3078\u306eAID system\u306e\u5c0e\u5165\u306b\u3088\u3063\u3066\u3001\u30aa\u30fc\u30ad\u30b7\u30f3\u5b58\u5728\u4e0b\u306b\u304a\u3044\u3066CENP-H\u306e\u767a\u73fe\u6291\u5236\u304c\u7d0430\u5206\u3067\u78ba\u8a8d\u3055\u308c\u3001FACS\u89e3\u6790\u306b\u3088\u3063\u30663\u6642\u9593\u4ee5\u5185\u306b\u7d30\u80de\u5468\u671f\u306e\u505c\u6b62\u304c\u958b\u59cb\u3055\u308c\u308b\u306e\u306b\u5bfe\u3057\u3066\u3001\u5f93\u6765\u306e\u65b9\u6cd5\uff08Tet-Offsystem\uff09\u3060\u3068\u7d30\u80de\u5468\u671f\u306e\u505c\u6b62\u304c\u958b\u59cb\u3055\u308c\u308b\u307e\u306736\u6642\u9593\u7a0b\u5ea6\u304b\u304b\u308b\u3053\u3068\u304b\u3089\u77ed\u6642\u9593\u306b\u52b9\u7387\u3088\u304f\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u9664\u53bb\u3067\u304d\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u539f\u8457\u8ad6\u6587\n<ul style=\"line-height: 1.5em;\">\n<li>Nishimura, K., et al. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat. Methods., 6, 917-922, 2009.<\/li>\n<li>Kanemaki, M.T. Frontiers of protein expression control with conditional degrons. Eur. J. Physiol., 465, 419-425, 2013.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><a name=\"EXR0013j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> YFP-FLAG-His\u30bf\u30b0\u4ed8\u304d\u767a\u73fe\u7528\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u7528\u3044\u305f\u5b9f\u9a13 (2015\/3\/13 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u306b\u304a\u3051\u308bGea1p\u306e\u6a5f\u80fd\u3092gea1p\u5909\u7570\u4f53\u3092\u7528\u3044\u3066\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u5f53\u958b\u767a\u5ba4\u304c\u63d0\u4f9b\u3057\u305f\u30ea\u30bd\u30fc\u30b9\u304c\u4f7f\u308f\u308c\u307e\u3057\u305f\u3002\n<ul style=\"line-height: 1.5em;\">\n<li>Gea1p\u306e\u7d30\u80de\u5185\u5c40\u5728\u3068\u904e\u5270\u767a\u73fe\u306b\u3088\u308b\u8868\u73fe\u578b\u306e\u8a55\u4fa1\uff08SpYFH30G11\u304a\u3088\u3073pDualYFH1c\u30d9\u30af\u30bf\u30fc\uff09\u3002<\/li>\n<li>\u30a8\u30f3\u30c9\u30b5\u30a4\u30c8\u30fc\u30b7\u30b9\u6d3b\u6027\u5316\u56e0\u5b50\u3067\u3042\u308bGolgi-endosome\u30bf\u30f3\u30d1\u30af\u8ceaArf1p\u3068\u3001\u7d30\u80de\u819c\u30bf\u30f3\u30d1\u30af\u8ceaArf6p\u306e\u7d30\u80de\u5185\u5c40\u5728\u306e\u89b3\u5bdf\uff08SpYFH39H12,SpYFH13E01\uff09\u3002<\/li>\n<li>ER\u30de\u30fc\u30ab\u30fc\uff08Sac11p\uff09\u304a\u3088\u3073Golgi\u30de\u30fc\u30ab\u30fc\uff08Sac12p\uff09\u306e\u7d30\u80de\u5185\u5c40\u5728\u306e\u89b3\u5bdf\uff08SpYFH26D10\u3001SpYFH36G05)\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u8ad6\u6587<br \/>\nEckler AM, Wilder C, Castanon A, Ferris VM, Lamere RA, Perrin BA, Pearlman R, White B, Byrd C, Ludvik N, Nichols N, Poole-Sumrall K, Sztul E, Styers ML. Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One. 8 (2): e56807 (2013).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06151\">pDUAL-YFH1c (cat# RDB06151)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0054j\">S.pombe ORFeome clones. YFP-FLAG-His6-tagged ORF clones (SpYFH)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0012j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u5909\u7570\u578bJAK2\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u305f\u3081\u306e\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u69cb\u7bc9 (2015\/2\/27 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>JAK2\u907a\u4f1d\u5b50\u306e\u5909\u7570\u4f53JAK2-V614F\u3092BaF3\/E\u7d30\u80de\u3078\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066pRx nZ ires Neo\u30d9\u30af\u30bf\u30fc\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nNagao T, Kurosu T, Umezawa Y, Nogami A, Oshikawa G, Tohda S, Yamamoto M, Miura O., Proliferation and survival signaling from both Jak2-V617F and Lyn involving GSK3 and mTOR\/p70S6K\/4EBP1 in PVTL-1 cell line newly established from acute myeloid leukemia transformed from polycythemia vera. PLoS One 9 (1): e84746 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01699\">pRx nZ ires Neo (cat# RDB01699)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0011j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> RepSTK1\u306e\u7d50\u6676\u69cb\u9020\u89e3\u6790 (2015\/2\/6 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>pSET33\u30d9\u30af\u30bf\u30fc\u5185\u306eRepSTK1\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u304c\u7d50\u6676\u69cb\u9020\u89e3\u6790\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nCarr SB, Mecia LB, Phillips SE, Thomas CD. Dentification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69 (Pt 10):1123-6 (2013).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB00920\">pSTE33 (cat# RDB00920)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0018j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u7cd6\u8f38\u9001\u4f53\u30bf\u30f3\u30d1\u30af\u8ceaSemiSWEET\u306e\u7acb\u4f53\u69cb\u9020 (2015\/1\/26 N.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662\u7406\u5b66\u7cfb\u7814\u7a76\u79d1\u306e\u6fe1\u6728 \u7406\u6559\u6388\u3001\u77f3\u8c37 \u9686\u4e00\u90ce\u51c6\u6559\u6388\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u7d30\u80de\u819c\u3092\u901a\u904e\u3055\u305b\u3066\u7cd6\u5206\u5b50\u3092\u8f38\u9001\u3059\u308b\u5f79\u5272\u3092\u62c5\u3046SemiSWEET\u30bf\u30f3\u30d1\u30af\u8cea\u306einward-open\u3001\u306a\u3089\u3073\u306boutward-open\u306e\u4e21\u72b6\u614b\u306e\u7acb\u4f53\u69cb\u9020\u3092\u7d50\u6676\u69cb\u9020\u89e3\u6790\u306b\u3088\u308a\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.s.u-tokyo.ac.jp\/ja\/press\/2015\/01.html\">\u300c\u8f38\u9001\u4f53\u30bf\u30f3\u30d1\u30af\u8ceaSemiSWEET\u304c\u7cd6\u5206\u5b50\u3092\u900f\u904e\u3055\u305b\u308b\u3057\u304f\u307f\u300d2015\u5e741\u670819\u65e5\u4ed8\u3000\u6771\u4eac\u5927\u5b66\u7406\u5b66\u90e8\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u8ad6\u6587<br \/>\nLee, Y., Nishizawa, T., Yamashita, K., Ishitani, R., Nureki, O. Structural basis for the facilitative diffusion mechanism by SemiSWEET transporter. Nat. Commun. 6: 6112 (2015).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/JGD07547\">Genomic DNA of Escherichia coli K-12, JCM20135 (Cat# JGD07547)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0010j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u304c\u3093\u7d30\u80de\u7279\u7570\u7684\u306aHSV-TK\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9 (2015\/1\/23 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7d30\u80de\u6bd2\u6027\u3092\u6301\u3064HSV-TK\u907a\u4f1d\u5b50\u3092\u304c\u3093\u7d30\u80de\u7279\u7570\u7684\u306b\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u3001\u672c\u30d7\u30e9\u30b9\u30df\u30c9\u306e HSV-TK \u907a\u4f1d\u5b50\u3092AFR\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u4e0b\u6d41\u306b\u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3001\u3055\u3089\u306b\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u306b\u7d44\u307f\u8fbc\u3093\u3067\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066E2F1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u4e0b\u6d41\u306b\u3082HSV-TK \u907a\u4f1d\u5b50\u3092\u30b5\u30d6\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3066\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKurayoshi K, Ozono E, Iwanaga R, Bradford AP, Komori H, Ohtani K. Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochem Biophys Res Commun. 450(1):240-246 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01027\">pTK5 (cat# RDB01027)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0005j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Sumoylation assay (2015\/1\/9 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>HEK293T\u7d30\u80de\u3067\u306eSTAT1\u53ca\u3073PML\u306eSumoylation assay\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nGur I, Fujiwara K, Hasegawa K, Yoshikawa K. Necdin promotes ubiquitin-dependent degradation of PIAS1 SUMO E3 ligase. PLoS One, 9 (6): e99503 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06126\">pRSFlag_mmSumo1 (cat# RDB06126)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0004j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u9175\u6bcdS. cerevisiae\u7528\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc(2014\/12\/12 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>S.cerevisiae\u3067Gal1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u5236\u5fa1\u4e0b\u3067\u306e\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u306b\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u672c\u7814\u7a76\u3067\u306f\u30a2\u30b4\u30cb\u30b9\u30c8\u63a2\u7d22\u306e\u305f\u3081\u306bEGFR\u3092\u9175\u6bcd\u3067\u767a\u73fe\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nYoshimoto N, Tatematsu K, Iijima M, Niimi T, Maturana AD, Fujii I, Kondo A, Tanizawa K, Kuroda S. High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system. Sci Rep., 4: 4242 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01956\">pGMH20 (cat# RDB01956)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0003j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> STAT3\u306e\u5f37\u5236\u767a\u73fe(2014\/11\/29 K.N.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>HEK293T\u7d30\u80de\u306b\u767a\u73fe\u3055\u305b\u3001IL6\u523a\u6fc0\u306b\u3088\u3063\u3066\u5f15\u304d\u8d77\u3053\u3055\u308c\u308bSTAT3\u3068IL6\u30b7\u30b0\u30ca\u30eb\u53d7\u5bb9\u4f53gp130\u3068\u306e\u7d50\u5408\u3001\u3055\u3089\u306bTRAF5\u306b\u3088\u308b\u963b\u5bb3\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nNagashima H, Okuyama Y, Asao A, Kawabe T, Yamaki S, Nakano H, Croft M, Ishii N, So T. The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6. Nat. Immunol., 15 (5): 449-456 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02353\">pEF-Flag-mSTAT3 (wild) (cat# RDB02353)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0007j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> WEE1\u5909\u7570\u4f53\u30b7\u30ea\u30fc\u30ba\u306e\u69cb\u7bc9 (2014\/11\/19 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>AKT\u306b\u3088\u308bWEE1\u306e\u30ea\u30f3\u9178\u5316\u3068\u305d\u306e\u5f71\u97ff\u3092\u89e3\u6790\u3059\u308b\u305f\u3081\u306e\u30d2\u30c8WEE1\u306e\u90e8\u5206\u6b20\u5931\u578b\u53ca\u3073\u30a2\u30df\u30ce\u9178\u7f6e\u63db\u578b\u5909\u7570\u4f53\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u4f5c\u88fd\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nKatayama K., Fujita N., Tsuruo T. Akt\/protein kinase B-dependent phosphorylation and inactivation of WEE1Hu promote cell cycle progression at G2\/M transition.\u3000Mol. Cell. Biol., 25, 5725-5237 (2005).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01204\"> WEE1Hu (cat# RDB01204)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0008j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> IL-1\u03b1\u767a\u73fe\u691c\u51fa\u306e\u30d7\u30ed\u30fc\u30d6 (2014\/11\/19 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>in situ\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u3092\u7528\u3044\u305fIL-1\u03b1 \u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u89e3\u6790\u3092\u884c\u3046\u305f\u3081\u306e\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u306e\u4f5c\u88fd\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nShayakhmetov D.M., Li Z.Y., Ni S., Lieber A. Interference with the IL-1-signaling pathway improves the toxicity profile of systemically applied adenovirus vectors. J. Immunol., 174, 7310-7319 (2005).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01516\"> pCAmsIL1 alpha (cat# RDB01516)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0009j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> RBPJ\u306b\u3088\u308bHES1\u907a\u4f1d\u5b50\u8ee2\u5199\u6291\u5236 (2014\/11\/19 T.M.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>Notch intracellular domain\u306b\u3088\u308bHES1\u8ee2\u5199\u6d3b\u6027\u5316\u304cRBPJ\uff08\u8ad6\u6587\u4e2d\u3067\u306fCBF1)\u306b\u4f9d\u5b58\u3057\u305f\u30d1\u30b9\u30a6\u30a7\u30a4\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3059\u305f\u3081\u306eRBPJ\u306e\u5f37\u5236\u767a\u73fe\u306bpCMX-N\/RBP-J \u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nSakamoto K, Chao WS, Katsube K, Yamaguchi A. Distinct roles of EGF repeats for the Notch signaling system. Exp. Cell Res. 302 (2): 281-291 (2005).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03021\"> pCMX-N\/RBP-J (cat# RDB03021)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0002j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u6f22\u65b9\u85ac\u306e\u8ee2\u5199\u3078\u306e\u5f71\u97ff\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0(2014\/11\/14 M.O.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u6f22\u65b9\u85ac\u3092\u4f5c\u7528\u3055\u305b\u305f\u30d2\u30c8\u4e73\u304c\u3093\u7d30\u80de\u682aMCF-7\u7d30\u80de\u3067\u306e\u304c\u3093\u95a2\u9023\u907a\u4f1d\u5b50 ERBB2\u3068ESR1\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u6d3b\u6027\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\nChiu JH, Chang CJ, Wu JC, Liu HJ, Wen CS, Hsu CH, Chen JL, Tseng LM, Chen WS, Shyr YM. Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF-7 Cell Line. Evid. Based Complement. Alternat. Med., 965486 (2014).<\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02839\">c-erbB2 promoter(533)-pGL2-basic (cat# RDB02839)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07528\">pGL4-phESR1 (cat# RDB07528)<\/a><\/li>\n<\/ul>\n<p><a name=\"EXR0001j\"><\/a><br \/>\n<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> Gli1 \u306b\u3088\u308b\u8ee2\u5199\u6d3b\u6027\u5316\u306e\u30ec\u30dd\u30fc\u30bf\u30fc (2014\/11\/07 T.Y.)<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30d2\u30c8\u30d0\u30ec\u30c3\u30c8\u98df\u9053\u8868\u76ae\u7d30\u80de\u3092\u7528\u3044Gli1\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30b7\u30e7\u30f3\u30a2\u30af\u30c1\u30d9\u30fc\u30bf\u30fc\u304cGli\u7d50\u5408\u9818\u57df\u3092\u6d3b\u6027\u5316\u3059\u308b\u304b\u8abf\u3079\u308b\u305f\u3081\u306b8&#215;3&#8217;Gli-BS-delta51-LucII\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<br \/>\n\u307e\u305f\u3001\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u30668xm3&#8217;Gli-BS-delta51-LucII\u304c\u4f7f\u7528\u3055\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u8ad6\u6587<br \/>\n<span style=\"color: #000000;\">Wang DH, Tiwari A, Kim ME, Clemons NJ, Regmi NL, Hodges WA, Berman DM, Montgomery EA, Watkins DN, Zhang X, Zhang Q, Jie C, Spechler SJ, Souza RF. Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett&#8217;s metaplasia.\u3000J. Clin. Invest. 124 (9): 3767-3780 (2014).<\/span><\/li>\n<li>\u4f7f\u7528\u3055\u308c\u305f\u30ea\u30bd\u30fc\u30b9<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08061\"> 8&#215;3&#8217;Gli-BS-delta51-LucII (cat# RDB08061)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08062\"> 8xm3&#8217;Gli-BS-delta51-LucII (cat# RDB08062)<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u5b9f\u9a13\u7528\u30de\u30a6\u30b9\u306f\u30db\u30eb\u30e2\u30f3\u300c\u30e1\u30e9\u30c8\u30cb\u30f3\u300d\u3092\u4f5c\u3089\u305a\u65e9\u719f\u306b<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u30de\u30a6\u30b9\u3067\u306f\u898b\u3064\u304b\u3063\u3066\u3044\u306a\u304b\u3063\u305f\u30e1\u30e9\u30c8\u30cb\u30f3\u5408\u6210\u7d4c\u8def\u306e\u9175\u7d20\u907a\u4f1d\u5b50\u306e\u4e00\u3064\u3001Hiomt\u907a\u4f1d\u5b50\u3092\u767a\u898b\u3057\u3001\u7406\u7814\uff22\uff32\uff23\u304c\u4f53\u7cfb\u7684\u306b\u53ce\u96c6\u30fb\u4fdd\u5b58\u3092\u884c\u3063\u3066\u304d\u305f\u30de\u30a6\u30b9\u7cfb\u7d71\u3084MSM\/Ms BAC\u30af\u30ed\u30fc\u30f3\u306a\u3069\u306e\u7814\u7a76\u30ea\u30bd\u30fc\u30b9\u3092\u7528\u3044\u3001\u591a\u304f\u306e\u5b9f\u9a13\u7528\u30de\u30a6\u30b9\u7cfb\u7d71\u304c\u30e1\u30e9\u30c8\u30cb\u30f3\u3092\u4f5c\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u304f\u306a\u3063\u305f\u7406\u7531\u3092\u3001\u30b2\u30ce\u30e0\u306e\u69cb\u9020\u3068\u751f\u7406\u5b66\u7684\u306a\u6a5f\u80fd\u306e\u4e21\u9762\u304b\u3089\u660e\u3089\u304b\u306b\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u7406\u7814\u8133\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08\u5229\u6839\u5ddd\u9032\u30bb\u30f3\u30bf\u30fc\u9577\uff09\u7cbe\u795e\u75be\u60a3\u52d5\u614b\u7814\u7a76\u30c1\u30fc\u30e0\u306e\u7b20\u539f\u548c\u8d77\u526f\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3001\u52a0\u85e4\u5fe0\u53f2\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3001\u7406\u7814\uff22\uff32\uff23\u52d5\u7269\u5909\u7570\u52d5\u614b\u89e3\u6790\u6280\u8853\u958b\u767a\u30c1\u30fc\u30e0\u306e\u963f\u90e8\u8a13\u4e5f\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3001\u5b9f\u9a13\u52d5\u7269\u958b\u767a\u5ba4\u306e\u76ee\u52a0\u7530\u548c\u4e4b\u7814\u7a76\u54e1\u3001\u5409\u6728\u6df3\u5ba4\u9577\u306b\u3088\u308b\u5171\u540c\u7814\u7a76\u6210\u679c\u3067\u3059\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/press\/2010\/20100323\/index.html\">\u300c\u5b9f\u9a13\u7528\u30de\u30a6\u30b9\u306f\u98fc\u80b2\u820e\u3067\u9032\u5316\u3001\u30db\u30eb\u30e2\u30f3\u300c\u30e1\u30e9\u30c8\u30cb\u30f3\u300d\u3092\u4f5c\u3089\u305a\u65e9\u719f\u306b &#8211; \u7406\u7814\u304c\u4fdd\u6709\u3059\u308b\u4e16\u754c\u4e2d\u306e\u30de\u30a6\u30b9\u7cfb\u7d71\u306e\u7814\u7a76\u30ea\u30bd\u30fc\u30b9\u3092\u99c6\u4f7f\u3057\u3066\u767a\u898b -\u300d2010\u5e743\u670823\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/clonesetja\/msmbac\">MSM\/Ms \u30de\u30a6\u30b9 BAC \u30af\u30ed\u30fc\u30f3 &amp; \u30e9\u30a4\u30d6\u30e9\u30ea\u30fc<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/userpbl01_16.png\" \/> \u30de\u30a6\u30b9\u306e\u7d76\u671b\u884c\u52d5\u3092\u5236\u5fa1\u3059\u308b\u907a\u4f1d\u5b50\u3092\u767a\u898b<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u540d\u53e4\u5c4b\u5927\u5b66\u5927\u5b66\u9662\u751f\u547d\u8fb2\u5b66\u7814\u7a76\u79d1\u306e\u6d77\u8001\u539f\u53f2\u6a39\u6587\u6559\u6388\u304a\u3088\u3073\u5bcc\u7530\u6ecb\u5148\u751f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u540d\u57ce\u5927\u5b66\u85ac\u5b66\u90e8\u3001\u6771\u4eac\u90fd\u8001\u4eba\u7dcf\u5408\u7814\u7a76\u6240\u3001\u7406\u7814\uff22\uff32\uff23\u3001\u30b8\u30e3\u30d1\u30f3\u7cbe\u795e\u85ac\u7406\u7814\u7a76\u6240\u3068\u306e\u5171\u540c\u7814\u7a76\u3067\u3001\u30de\u30a6\u30b9\u306e\u300c\u7d76\u671b\u884c\u52d5\u300d\u3092\u5236\u5fa1\u3059\u308b\u907a\u4f1d\u5b50\u3092\u7279\u5b9a\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\nTomida, S. et al., Nat. Genet. 41: 688 &#8211; 695 (2009).<br \/>\n<a href=\"http:\/\/jc-sh.com\/2012website\/research\/pdf\/activities\/20090525_agr.pdf\">\u300c\u30de\u30a6\u30b9\u306e\u7d76\u671b\u884c\u52d5\u3092\u5236\u5fa1\u3059\u308b\u907a\u4f1d\u5b50\u3092\u767a\u898b\u300d2009\u5e7405\u670825\u65e5\u4ed8\u540d\u53e4\u5c4b\u5927\u5b66\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a>\u3001\u8aad\u58f2\u65b0\u805e\u3001\u4e2d\u65e5\u65b0\u805e\u3001\u65e5\u520a\u5de5\u696d\u65b0\u805e\u3001\u65e5\u672c\u7d4c\u6e08\u65b0\u805e<\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/clonesetja\/msmbac\">MSM\/Ms \u30de\u30a6\u30b9 BAC \u30af\u30ed\u30fc\u30f3 &amp; \u30e9\u30a4\u30d6\u30e9\u30ea\u30fc<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u300c\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u300d\u306e\u8f38\u9001\u306b\u5fc5\u9808\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u3092\u69cb\u9020\u6c7a\u5b9a<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u7814\u751f\u547d\u5206\u5b50\u30b7\u30b9\u30c6\u30e0\u57fa\u76e4\u7814\u7a76\u9818\u57df\u306f\u3001\u9ad8\u30a8\u30cd\u30eb\u30ae\u30fc\u52a0\u901f\u5668\u7814\u7a76\u6a5f\u69cb\u3068\u3068\u3082\u306b\u3001\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u306e\u8f38\u9001\u306b\u5fc5\u9808\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u300cRab27\u300d\u306e2\u3064\u306e\u8907\u5408\u4f53\u306e\u69cb\u9020\u3092\u4e16\u754c\u3067\u521d\u3081\u3066\u89e3\u660e\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.tohoku.ac.jp\/japanese\/press_release\/pdf2008\/20081008.pdf\">\u300c\u300e\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u300f\u306e\u8f38\u9001\u306b\u5fc5\u9808\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u3092\u69cb\u9020\u6c7a\u5b9a &#8211; \u808c\u306e\u7f8e\u767d\u7dad\u6301\u3084\u767d\u9aea\u6291\u5236\u306a\u3069\u306e\u85ac\u5264\u958b\u767a\u306b\u671f\u5f85 -\u300d2008\u5e7410\u670803\u65e5\u4ed8\u6771\u5317\u5927\u5b66\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2008\/20081008_1\/20081008_1.pdf\">2008\u5e7410\u670808\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03423\">Rab27A (RDB03423)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03422\">Slac2-a (RDB03422)<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u751f\u547d\u306b\u5371\u6a5f\u304c\u8feb\u308b\u3068\u6a5f\u80fd\u3059\u308b\u3001\u9ad8\u5ea6\u597d\u71b1\u83cc\u306e\u65b0\u898f\u8ee2\u5199\u56e0\u5b50\u3092\u767a\u898b<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u7814\u653e\u5c04\u5149\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u653e\u5c04\u5149\u30b7\u30b9\u30c6\u30e0\u751f\u7269\u5b66\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\uff08\u5009\u5149\u6210\u7d00\u30b0\u30eb\u30fc\u30d7\u30c7\u30a3\u30ec\u30af\u30bf\u30fc\uff09\u306f\u3001\u5897\u6b96\u306e\u5b9a\u5e38\u671f\u306b\u767a\u73fe\u91cf\u304c\u5897\u52a0\u3057\u3001\u98e2\u9913\u3084\u9178\u5316\u30b9\u30c8\u30ec\u30b9\u306a\u3069\u306b\u5bfe\u6297\u3059\u308b\u305f\u3081\u306e14\u500b\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u5236\u5fa1\u3059\u308b\u65b0\u898f\u8ee2\u5199\u56e0\u5b50\u300cSdrP(Stationary-phase Dependent Regulatory Protein)\u300d\u3092\u767a\u898b\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u4e0a\u5229\u4f73\u5f18\u30ea\u30b5\u30fc\u30c1\u30a2\u30bd\u30b7\u30a8\u30a4\u30c8\u3001\u65b0\u6d77\u6681\u7537\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304c\u300c\u9ad8\u5ea6\u597d\u71b1\u83cc\u4e38\u3054\u3068\u4e00\u5339\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u300d\u3067\u884c\u3063\u305f\u7814\u7a76\u6210\u679c\u3067\u3059\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2008\/20080828_1\/20080828_1.pdf\">\u300c\u751f\u547d\u306b\u5371\u6a5f\u304c\u8feb\u308b\u3068\u6a5f\u80fd\u3059\u308b\u3001\u9ad8\u5ea6\u597d\u71b1\u83cc\u306e\u65b0\u898f\u8ee2\u5199\u56e0\u5b50\u3092\u767a\u898b &#8211; \u98e2\u9913\u3084\u9178\u5316\u30b9\u30c8\u30ec\u30b9\u306b\u5bfe\u6297\u3059\u308b\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u4fc3\u9032 -\u300d2008\u5e7408\u670828\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/thermus\">Thermus thermophilus plasmid<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u300e\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u300f\u306e\u8f38\u9001\u3092\u963b\u5bb3\u3059\u308b\u65b0\u9175\u7d20\u767a\u898b<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u7814\u798f\u7530\u72ec\u7acb\u4e3b\u5e79\u30e6\u30cb\u30c3\u30c8\u3068\u6771\u5317\u5927\u5b66\u5927\u5b66\u9662\u751f\u547d\u79d1\u5b66\u7814\u7a76\u79d1\u819c\u8f38\u9001\u6a5f\u69cb\u89e3\u6790\u5206\u91ce\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u9175\u7d20\u300cRab27A-GAP\u300d\u304c\u3001\u30e1\u30e9\u30ce\u30bd\u30fc\u30e0\u8f38\u9001\u306b\u304a\u3044\u3066\u8377\u672d\u306e\u3088\u3046\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u300cRab27A\u300d\u3092\u7279\u7570\u7684\u306b\u4e0d\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u3067\u3001\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u306e\u8f38\u9001\u3092\u963b\u5bb3\u3059\u308b\u6a5f\u80fd\u304c\u3042\u308b\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2006\/20060914_1\/220060914_1pdf.pdf\">\u300c\u300e\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u300f\u306e\u8f38\u9001\u3092\u963b\u5bb3\u3059\u308b\u65b0\u9175\u7d20\u767a\u898b &#8211; \u76ae\u819a\u306e\u6697\u8272\u5316\u5236\u5fa1\u3092\u884c\u3046\u5206\u5b50\u6a19\u7684\u3068\u3057\u3066\u671f\u5f85 -\u300d2006\u5e7409\u670814\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><br \/>\n<a href=\"http:\/\/www.lifesci.tohoku.ac.jp\/research_ja\/1126\/\">2006\u5e7409\u670814\u65e5\u4ed8\u6771\u5317\u5927\u5b66\u5927\u5b66\u9662\u751f\u547d\u79d1\u5b66\u7814\u7a76\u79d1\u30c8\u30d4\u30c3\u30af\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03423\">Rab27A (RDB03423)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03422\">Slac2-a (RDB03422)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03421\">Slp4-a (RDB03421)<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u5206\u88c2\u9175\u6bcd\u4e38\u3054\u3068\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6271\u3046\u89e3\u6790\u7cfb\u3092\u78ba\u7acb<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u4e2d\u592e\u7814\u7a76\u6240\u306e\u5409\u7530\u5316\u5b66\u907a\u4f1d\u5b66\u7814\u7a76\u5ba4\u306f\u3001\u4eba\u9593\u3068\u540c\u3058\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6570\u591a\u304f\u6301\u3061\u3001\u5358\u7d14\u3067\u6271\u3044\u3084\u3059\u3044\u201c\u5206\u88c2\u9175\u6bcd\u201d\u306b\u3064\u3044\u3066\u3001DNA\u914d\u5217\u304b\u3089\u4e88\u6e2c\u3055\u308c\u308b\u907a\u4f1d\u5b50\u306e\u307b\u3068\u3093\u3069\u3092\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u7d44\u307f\u8fbc\u3093\u3060\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u69cb\u7bc9\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2006\/20060626_1\/20060626_1.pdf\">\u300c\u5206\u88c2\u9175\u6bcd\u4e38\u3054\u3068\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6271\u3046\u89e3\u6790\u7cfb\u3092\u78ba\u7acb &#8211; \u30b2\u30ce\u30e0\u89e3\u6790\u306f\u300c\u60c5\u5831\u300d\u304b\u3089\u300c\u30e2\u30ce\u300d\u3078  -\u300d2006\u5e7406\u670826\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0054j\">S. pombe ORFeome Clones<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u30d2\u30c811\u756a\u67d3\u8272\u4f53\u306e\u907a\u4f1d\u5b50\u30ab\u30bf\u30ed\u30b0\u3092\u4f5c\u6210<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u56fd\u969b\u30c1\u30fc\u30e0\u306e\u4e2d\u5fc3\u7684\u5f79\u5272\u3092\u679c\u305f\u3057\u305f\u7406\u7814\u30b2\u30ce\u30e0\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306f\u3001\u7cd6\u5c3f\u75c5\u3084\u767d\u8840\u75c5\u306a\u3069\u75be\u60a3\u95a2\u9023\u6027\u304c\u9ad8\u3044\u30d2\u30c811\u756a\u67d3\u8272\u4f53\u306b\u3064\u3044\u3066\u3001\u3055\u3089\u306b\u9ad8\u7cbe\u5ea6\u306e\u89e3\u6790\u3092\u7d9a\u3051\u30011,524\u500b\u306e\u907a\u4f1d\u5b50\u7523\u7269\u306e\u69cb\u9020\u3068\u7a2e\u985e\u306a\u3069\u306e\u60c5\u5831\u3092\u7db2\u7f85\u3057\u305f\u30ab\u30bf\u30ed\u30b0\u3092\u4f5c\u6210\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2006\/20060323_1\/20060323_1.pdf\">\u300c\u30d2\u30c8 11 \u756a\u67d3\u8272\u4f53\u306e\u907a\u4f1d\u5b50\u30ab\u30bf\u30ed\u30b0\u3092\u4f5c\u6210 &#8211; \u75be\u60a3\u95a2\u9023\u6027\u304c\u9ad8\u3044\u30d2\u30c8 11 \u756a\u67d3\u8272\u4f53\u7814\u7a76\u306b\u52a0\u901f -\u300d2006\u5e7403\u670823\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/hum21ch\">Human chromosome genomic clones<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u300e\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u300f\u306e\u8f38\u9001\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u660e<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u7814\u798f\u7530\u72ec\u7acb\u4e3b\u5e79\u7814\u7a76\u30e6\u30cb\u30c3\u30c8\u306e\u9ed2\u7530\u5782\u6b69\u57fa\u790e\u79d1\u5b66\u7279\u5225\u7814\u7a76\u54e1\u3068\u798f\u7530\u5149\u5247\u30e6\u30cb\u30c3\u30c8\u30ea\u30fc\u30c0\u30fc\u306f\u3001\u30e1\u30e9\u30ce\u30b5\u30a4\u30c8\uff08\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u7523\u751f\u7d30\u80de\uff09\u304c\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u3092\u8f38\u9001\u306b\u3064\u3044\u3066\u3001\u819c\u8f38\u9001\u306e\u5236\u5fa1\u306b\u95a2\u308f\u308b\u4f4e\u5206\u5b50\u91cfG\u30bf\u30f3\u30d1\u30af\u8ceaRab27A\u306b\u7d50\u5408\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u5206\u5b50Slac2-a\u53ca\u3073Slp2-a\u306e\u6a5f\u80fd\u963b\u5bb3\u5b9f\u9a13\u306b\u3088\u308a\u3001\u30e1\u30e9\u30cb\u30f3\u8f38\u9001\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2004\/20041115_1\/20041115_1.pdf\">\u300c\u300e\u30e1\u30e9\u30cb\u30f3\u8272\u7d20\u300f\u306e\u8f38\u9001\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u660e &#8211; \u808c\u3084\u9aea\u306e\u6bdb\u304c\u9ed2\u304f\u306a\u308b\u4ed5\u7d44\u307f -\u300d2004\u5e7411\u670815\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03423\">Rab27A (RDB03423)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03422\">Slac2-a (RDB03422)<\/a><br \/>\n<a href=\"https:\/\/brc.riken.jp\/dna\/RDB03421\">Slp4-a (RDB03421)<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u9ad8\u7cbe\u5ea6\u30d2\u30c8\u30b2\u30ce\u30e0\u306b\u95a2\u3059\u308b\u5b66\u8853\u8ad6\u6587\u306e\u767a\u8868<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u65e5\u3001\u7c73\u3001\u82f1\u3001\u4ecf\u3001\u72ec\u3001\u4e2d\u306e6\u304b\u56fd18\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7b49\u304b\u3089\u69cb\u6210\u3055\u308c\u308b\u3001\u56fd\u969b\u30d2\u30c8\u30b2\u30ce\u30e0\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\u30b3\u30f3\u30bd\u30fc\u30b7\u30a2\u30e0(IHGSC\uff1a International Human Genome Sequencing Consortium)\u306f\u3001\u30d2\u30c8\u30b2\u30ce\u30e0\u306e\u9ad8\u7cbe\u5ea6\u914d\u5217\u306e\u6700\u7d42\u7684\u306a\u691c\u8a3c\u3068\u89e3\u6790\u3092\u884c\u3044\u3001\u305d\u306e\u6210\u679c\u3092\u5b66\u8853\u8ad6\u6587\u3068\u3057\u3066\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u6211\u304c\u56fd\u3067\u306f\u3001\u72ec\u7acb\u884c\u653f\u6cd5\u4eba\u7406\u5316\u5b66\u7814\u7a76\u6240\u30b2\u30ce\u30e0\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08GSC\uff09\u306e\u698a\u4f73\u4e4b\u30bb\u30f3\u30bf\u30fc\u9577\uff08\u56fd\u969b\u30d2\u30c8\u30b2\u30ce\u30e0\u6a5f\u69cb\u524d\u4f1a\u9577\uff09\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304c\u4e2d\u5fc3\u3068\u306a\u308a\u3001\u914d\u5217\u306e\u5b8c\u6210\u53ca\u3073\u89e3\u6790\u306a\u3069\u4eca\u56de\u306e\u6210\u679c\u306b\u5927\u304d\u304f\u8ca2\u732e\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2004\/20041021_1\/20041021_1.pdf\">\u300c\u9ad8\u7cbe\u5ea6\u30d2\u30c8\u30b2\u30ce\u30e0\u306b\u95a2\u3059\u308b\u5b66\u8853\u8ad6\u6587\u306e\u767a\u8868\u300d2004\u5e7410\u670821\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/hum21ch\">Human chromosome genomic clones<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u9ad8\u5ea6\u597d\u71b1\u83cc\u30ea\u30f3\u9178\u30de\u30f3\u30ce\u30fc\u30b9\u8ee2\u79fb\u9175\u7d20\u306e\u69cb\u9020\u89e3\u6790\u306b\u6210\u529f<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u7814\u64ad\u78e8\u7814\u7a76\u6240\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u30d5\u30a1\u30af\u30c8\u30ea\u30fc\u69cb\u9020\u89e3\u6790\u7b2c\uff12\u7814\u7a76\u30c1\u30fc\u30e0\u306e\u56fd\u5cf6\u76f4\u6a39\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3068\u86cb\u767d\u8cea\u69cb\u9020\u89e3\u6790\u30b3\u30f3\u30bd\u30fc\u30b7\u30a2\u30e0\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u9ad8\u5ea6\u597d\u71b1\u83cc <i>Thermus thermophilus<\/i>HB8 \u304c\u7523\u751f\u3059\u308b\u8010\u71b1\u6027\u306e\u30ea\u30f3\u9178\u30de\u30f3\u30ce\u30fc\u30b9\u8ee2\u79fb\u9175\u7d20(Phosphomannomutase)\u306e\u7acb\u4f53\u69cb\u9020\u3092\u6c7a\u5b9a\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2004\/20040709_1\/20040709_1.pdf\">\u300c\u9ad8\u5ea6\u597d\u71b1\u83cc\u30ea\u30f3\u9178\u30de\u30f3\u30ce\u30fc\u30b9\u8ee2\u79fb\u9175\u7d20\u306e\u69cb\u9020\u89e3\u6790\u306b\u6210\u529f  &#8211; \u86cb\u767d\u30b3\u30f3\u30bd\u3068\u306e\u521d\u306e\u5171\u540c\u7814\u7a76\u6210\u679c -\u300d2004\u5e7407\u670809\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/thermus\">Thermus thermophilus Expression plasmid<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u30d2\u30c8\u30b2\u30ce\u30e0\u306e\u30c9\u30e9\u30d5\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c\u3092\u516c\u8868<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u5316\u5b66\u7814\u7a76\u6240\u6a2a\u6d5c\u7814\u7a76\u6240\u30b2\u30ce\u30e0\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08\uff27\uff33\uff23\uff09\u306e\u30b2\u30ce\u30e0\u69cb\u9020\u60c5\u5831\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\uff08\u698a\u4f73\u4e4b\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u30c7\u30a3\u30ec\u30af\u30bf\u30fc\uff09\u306f\u3001\u6771\u4eac\u5927\u5b66\u3068\u5171\u540c\u3067\u3001\u30b2\u30ce\u30e0\u60c5\u5831\u3092\u52b9\u679c\u7684\u306b\u5229\u7528\u3059\u308b\u305f\u3081\u306e\u300c\u30d2\u30c8\u30b2\u30ce\u30e0\u30c9\u30e9\u30d5\u30c8\u914d\u5217\u30c7\u30fc\u30bf\u30fc\u30d9\u30fc\u30b9\u300d\u3092\u4e16\u754c\u306b\u5148\u99c6\u3051\u3066\u958b\u767a\u3057\u3001\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u3067\u516c\u958b\u3057\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u30d2\u30c8\u30b2\u30ce\u30e0\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u6c7a\u5b9a\u306b\u5927\u304d\u304f\u8ca2\u732e\u3057\u3001\u7d04\uff12\uff10\uff13\uff2d\uff42\u306e\u30c7\u30fc\u30bf\u3092\u53d6\u5f97\u3057\u3001\uff11\uff10\uff10\uff2d\uff42\u4ee5\u4e0a\u306e\u30c7\u30fc\u30bf\u89e3\u6790\u3092\u884c\u3063\u305f\uff16\u7814\u7a76\u6a5f\u95a2\u306e\u4e00\u3064\u306b\u6570\u3048\u3089\u308c\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2001\/20010212_1\/20010212_1.pdf\">\u300c\u30d2\u30c8\u30b2\u30ce\u30e0\u306e\u30c9\u30e9\u30d5\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c\u3092\u516c\u8868\u300d2001\u5e7402\u670812\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/hum21ch\">Human chromosome genomic clones<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u30d2\u30c8\u30b2\u30ce\u30e0\u30c9\u30e9\u30d5\u30c8\u914d\u5217\u7d71\u5408\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u4f5c\u6210\u30fb\u516c\u958b<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u5316\u5b66\u7814\u7a76\u6240\u30b2\u30ce\u30e0\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08\uff27\uff33\uff23\uff09\u30fb\u30b2\u30ce\u30e0\u69cb\u9020\u60c5\u5831\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\uff08\u698a\u4f73\u4e4b\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u30c7\u30a3\u30ec\u30af\u30bf\u30fc\u3001\u77e2\u7530\u54f2\u58eb\u7814\u7a76\u54e1\u307b\u304b\uff09\u3068\u3001\u6771\u4eac\u5927\u5b66\u533b\u79d1\u5b66\u7814\u7a76\u6240\u30d2\u30c8\u30b2\u30ce\u30e0\u89e3\u6790\u30bb\u30f3\u30bf\u30fc\u30fb\u30b2\u30ce\u30e0\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u5206\u91ce\uff08\u9ad8\u6728\u5229\u4e45\u6559\u6388\uff09\u306f\u5171\u540c\u3067\u3001\u30c9\u30e9\u30d5\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u304c\u7d42\u4e86\u3057\u305f\u300c\u30d2\u30c8\u30b2\u30ce\u30e0\u300d\u306e\u914d\u5217\u60c5\u5831\u3092\u30b2\u30ce\u30e0\u7814\u7a76\u306b\u5229\u7528\u3057\u3084\u3059\u3044\u5f62\u5f0f\u3067\u516c\u958b\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u306b\u3001\u65b0\u3057\u3044\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u4f5c\u6210\u3057\u3001\u4e21\u30bb\u30f3\u30bf\u30fc\u306e\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u4e0a\u3067\u516c\u958b\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"http:\/\/www.riken.jp\/~\/media\/riken\/pr\/topics\/2000\/20000717_1\/20000717_1.pdf\">2000\u5e7407\u670817\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/hum21ch\">Human chromosome genomic clones<\/a><\/li>\n<\/ul>\n<p><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/orgpaper01_16.png\" \/>\u30d2\u30c8\uff12\uff11\u756a\u67d3\u8272\u4f53\u306e\u89e3\u8aad\u5b8c\u4e86\u306b\u3064\u3044\u3066<\/p>\n<ul style=\"line-height: 1.5em;\">\n<li>\u7406\u5316\u5b66\u7814\u7a76\u6240\u306f\u3001\u56fd\u969b\u30d2\u30c8\u30b2\u30ce\u30e0\u8a08\u753b\u306e\u4e00\u74b0\u3068\u3057\u3066\u3001\u6176\u5fdc\u5927\u5b66\u533b\u5b66\u90e8\u53ca\u3073\u30c9\u30a4\u30c4\u306e\uff13\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u3068\u5408\u540c\u3067\u300121\u756a\u67d3\u8272\u4f53\u306e\uff24\uff2e\uff21\u5869\u57fa\u914d\u5217\u306e\u89e3\u8aad\u3092\u7d42\u3048\u305f\u3053\u3068\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u7406\u5316\u5b66\u7814\u7a76\u6240\u3067\u306f\u30b2\u30ce\u30e0\u79d1\u5b66\u7dcf\u5408\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\uff08\u548c\u7530\u662d\u5141\u30bb\u30f3\u30bf\u30fc\u6240\u9577\uff09\u30fb\u30b2\u30ce\u30e0\u69cb\u9020\u60c5\u5831\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u698a\u4f73\u4e4b\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u30c7\u30a3\u30ec\u30af\u30bf\u30fc\u304c\u4e2d\u5fc3\u3068\u306a\u308a\u3001\u7d04\uff13\u5343\uff14\u767e\u4e07\u5869\u57fa\u3067\u69cb\u6210\u3055\u308c\u308b21\u756a\u67d3\u8272\u4f53\u306e\u3046\u3061\u300150\uff05\u3092\u62c5\u5f53\u3057\u307e\u3057\u305f\u3002<\/li>\n<li>\u51fa\u5178<br \/>\n<a href=\"https:\/\/www.riken.jp\/medialibrary\/riken\/pr\/press\/2000\/20000508_1\/20000508_1.pdf\">\u300c\u30d2\u30c8 21 \u756a\u67d3\u8272\u4f53\u306e\u89e3\u8aad\u5b8c\u4e86\u306b\u3064\u3044\u3066\u300d2000\u5e7405\u670808\u65e5\u4ed8\u7406\u7814\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a><\/li>\n<li>\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u6750\u6599<br \/>\n<a href=\"\/ja\/hum21ch\">Human chromosome genomic clones<\/a><\/li>\n<\/ul>\n<p><a href=\"#top\">go to top<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>HIV-1\u611f\u67d3\u7d30\u80de\u3092\u7279\u5fb4\u3065\u3051\u308b\u907a\u4f1d\u5b50\u767a\u73fe (2021\/11\/01 T.M.) \u514d\u75ab\u306e\u53f8\u4ee4\u5854\u306e\u5f79\u5272\u3092\u679c\u305f\u3059\u30d2\u30c8 CD4 T\u7d30\u80de\u306f\u3001HIV-1\u306e\u4e3b\u305f\u308b\u611f\u67d3\u6a19\u7684\u3067\u3059\u3002\u672c\u8ad6\u6587\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001CD4 T\u7d30\u80de\u3092\u542b\u3080\u4eba\u306e\u514d\u75ab\u30b7\u30b9\u30c6\u30e0\u3092\u518d\u69cb\u7bc9\u3057\u305f\u300c\u30d2\u30c8\u5316\u30de\u30a6\u30b9\u300d\u306b\u30ec\u30dd\u30fc\u30bf\u30fc\u30a6\u30a4\u30eb\u30b9HIV1-GFP\u3092\u611f\u67d3\u3055\u305b\u3001\u7d30\u80de\u306e\u591a\u89d2\u7684\u89e3\u6790\u624b\u6cd5\u306b\u3088\u308aHIV-1\u7523\u51fa\u7d30\u80de\u3092\u7279\u5fb4\u3065\u3051\u308b\u8981\u56e0\u3068\u3057\u3066\u3001CXCL13\u907a\u4f1d\u5b50\u9ad8\u767a\u73fe\u96c6\u56e3\u3068\u30a4\u30f3\u30bf [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":1919,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-111","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/111","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=111"}],"version-history":[{"count":20,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/111\/revisions"}],"predecessor-version":[{"id":5790,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/111\/revisions\/5790"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1919"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=111"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}