{"id":9127,"date":"2026-03-25T16:02:11","date_gmt":"2026-03-25T07:02:11","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=9127"},"modified":"2026-03-31T16:22:45","modified_gmt":"2026-03-31T07:22:45","slug":"rtum","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/rtum","title":{"rendered":"High-Quality, High-Yield Plasmid Supply Service  \u201cReady to Use Maxi\u201d"},"content":{"rendered":"<div id=\"rtum\">\n<div class=\"highlight-box\">\n   The Gene Engineering Division (RIKEN DNA Bank) has launched a new service providing high-quality plasmids purified using the QIAGEN Plasmid Plus Maxi Kit. No preparation is required\u2014experiments such as transfection for cells can be started immediately upon arrival.\n<\/div>\n<h3>Highlights of Ready to Use Maxi<\/h3>\n<table class=\"spec-table\">\n<tr>\n<th>Reliable Quality<\/th>\n<td>\n<p>Full-length sequence verification for each lot using HTS (High-Throughput Sequencing)<br \/>\n         Endotoxin-reduced purification allowing direct use in cultured cells\n      <\/p>\n<\/td>\n<\/tr>\n<tr>\n<th>Sufficient yield<\/th>\n<td>\n<p>\n     50 \u00b5g provided (estimated from OD values)<br \/>\n     If preparation from a single kit yields less than 50 \u00b5g, the entire amount obtained will be provided.\n      <\/p>\n<\/td>\n<\/tr>\n<tr>\n<th>Selectable Solvent<\/th>\n<td>Choose from TE Buffer \/ 10 mM Tris-Cl (pH 8.5) \/ Nuclease-free water<\/td>\n<\/tr>\n<\/table>\n<h3>Fee (per plasmid)<\/h3>\n<table class=\"spec-table\">\n<tr>\n<th><\/th>\n<th style=\"text-align: center;\">Domestic<\/th>\n<th style=\"text-align: center;\">International<\/th>\n<\/tr>\n<tr>\n<th>For use in non-profit academic research<\/th>\n<td style=\"text-align: center;\">24,750 JPY (tax included)<\/td>\n<td style=\"text-align: center;\">49,500 JPY<\/td>\n<\/tr>\n<tr>\n<th>For use in for-profit or commercial research<\/th>\n<td style=\"text-align: center;\">49,500 JPY (tax included)<\/td>\n<td style=\"text-align: center;\">99,000 JPY<\/td>\n<\/tr>\n<\/table>\n<div class=\"shipping-note\">\n<p>\u2022 Shipping and packaging costs for the resources will be charged.<\/p>\n<p>\n    \u2022 For expression vectors carrying mammalian cDNA generated using the \u201cGateway\u00ae Technology\u201d, a license-equivalent fee (2,090 JPY per plasmid) will be added. For use by commercial institutions, a separate agreement with Life Technologies is required.<\/p>\n<div class=\"btn-center-container\">\n    <a href=\"https:\/\/forms.office.com\/r\/LTuVHwhCEC\" class=\"btn\" target=\"_blank\">Order information entry form<\/a><\/p>\n<\/div>\n<\/div>\n<h3>Information Provided with the Resource <\/h3>\n<p>The following information will also be provided:<\/p>\n<ul>\n<li>DNA concentration<\/li>\n<li>Solution volume<\/li>\n<li>OD260\/280 ratio<\/li>\n<li>Agarose gel electrophoresis image<\/li>\n<\/ul>\n<h3>Available Plasmids<\/h3>\n<p>The following plasmids are immediately available.<\/p>\n<p>\nOther plasmids may also be prepared upon request.  Please feel free to contact us.<br \/>\nE-mail: dnabank.brc@riken.jp\n<\/p>\n<ul class=\"plasmid-list\">\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB04\/RDB04394.html\" target=\"_blank\">pCAG-HIVgp (RDB04394)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB04\/RDB04393.html\" target=\"_blank\">pCMV-VSV-G-RSV-Rev (RDB04393)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB04\/RDB04392.html\" target=\"_blank\">pCMV-VSV-G (RDB04392)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB08\/RDB08121.html\" target=\"_blank\">pRSV-Rev (RDB08121)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18223.html\" target=\"_blank\">mito-SRAI_pcDNA3 (RDB18223)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19694.html\" target=\"_blank\">pAAV-eSyn-RCaMP3-WPRE (RDB19694)<\/a><\/li>\n<li><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB15\/RDB15446.html\" target=\"_blank\">tFucci(CA)2\/pCSII-EF (RDB15446)<\/a><\/li>\n<\/ul>\n<div class=\"btn-center-container\">\n      <a href=\"https:\/\/forms.office.com\/r\/LTuVHwhCEC\" class=\"btn\" target=\"_blank\">Order information entry form<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Gene Engineering Division (RIKEN DNA Bank) has launched a new service providing high-quality plasmids purified using the QIAGEN Plasmid Plus Maxi Kit. No preparation is required\u2014experiments such as transfection for cells can be started immediately upon arrival. Highlights of Ready to Use Maxi Reliable Quality Full-length sequence verification for each lot using HTS (High-Throughput Sequencing) Endotoxin-reduced purification allowing direct use in cultured cells Sufficient yield 50 \u00b5g provided (estimated from OD values) If preparation from a single kit yields less than 50 \u00b5g, the entire amount obtained will be provided. Selectable Solvent Choose from TE Buffer \/ 10 mM Tris-Cl (pH 8.5) \/ Nuclease-free water Fee (per plasmid) Domestic International For use in non-profit academic research 24,750 JPY (tax included) 49,500 JPY For use in for-profit or commercial research 49,500 JPY (tax included) 99,000 JPY \u2022 Shipping and packaging costs for the resources will be charged. \u2022 For expression vectors carrying mammalian cDNA generated using the \u201cGateway\u00ae Technology\u201d, a license-equivalent fee (2,090 JPY per plasmid) will be added. For use by commercial institutions, a separate agreement with Life Technologies is required. Order information entry form Information Provided with the Resource The following information will also be provided: DNA concentration [&hellip;]<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-9127","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/9127","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=9127"}],"version-history":[{"count":4,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/9127\/revisions"}],"predecessor-version":[{"id":9160,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/9127\/revisions\/9160"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=9127"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}