{"id":8936,"date":"2025-05-06T10:11:16","date_gmt":"2025-05-06T01:11:16","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8936"},"modified":"2025-05-06T10:12:39","modified_gmt":"2025-05-06T01:12:39","slug":"promoter01en","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/promoter01en","title":{"rendered":"Promoter Collection of DNA Bank"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h3>Plasmid constructed by the Gene Engineering Division<\/h3>\n<ul style=\"line-height: 1.5em;\">\n<li>We amplified upstream region of genes by PCR, cloned the amplified fragment into Renilla luciferase<img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/> and firefly luciferase<img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/> vectors, respectively, and then their terminal sequences were confirmed.<\/li>\n<li><a href=\"\/en\/promoter01en\/pr_renillalucen\">Renilla luciferase collection<img decoding=\"async\" alt=\"Renilla\" src=\"https:\/\/dnaconda.riken.jp\/images\/Renilla.png\" \/><\/a> were analysed by using 18 cell lines.<\/li>\n<li><a href=\"\/en\/promoter01en\/pr_fireflylucen\">Firefly luciferase collection<img decoding=\"async\" alt=\"firefly\" src=\"https:\/\/dnaconda.riken.jp\/images\/firefly.png\" \/><\/a> were analysed by using HeLa, HepG2 and Hep3B cells.<\/li>\n<\/ul>\n<h3>Miscellaneous<\/h3>\n<ul style=\"line-height: 1.5em;\">\n<li><a href=\"\/en\/promoter01en\/pr_elementen\">Binding Element<\/a><\/li>\n<\/ul>\n<p style=\"text-align:left;\">2025.05.06 (T.M. GRP0013e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Plasmid constructed by the Gene Engineering Division We amplified upstream region of genes by PCR, cloned the amplified fragment into Renilla luciferase and firefly luciferase vectors, respectively, and then their terminal sequences were confirmed. Renilla luciferase collection were analysed by using 18 cell lines. Firefly luciferase collection were analysed by using HeLa, HepG2 and Hep3B cells. Miscellaneous Binding Element 2025.05.06 (T.M. GRP0013e)<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8936","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8936","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8936"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8936\/revisions"}],"predecessor-version":[{"id":8938,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8936\/revisions\/8938"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8936"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}