{"id":8921,"date":"2025-05-06T10:03:58","date_gmt":"2025-05-06T01:03:58","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8921"},"modified":"2025-05-06T10:04:47","modified_gmt":"2025-05-06T01:04:47","slug":"mblfp01en","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/curated_topicsen\/mblfp01en","title":{"rendered":"<i>CoralHue<\/i>&trade; Fluorescent Protein Resources"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h3>Distribution of <i>CoralHue<\/i>&trade; Fluorescent Protein Resources<\/h3>\n<p>With the generosity of <a href=\"https:\/\/www.mblbio.com\/e\/\">Medical &#038; Biological Laboratories Co., Ltd. (MBL)<\/a>, 88 items of <i>CoralHue<\/i>&trade; fluorescent protein resources were deposited to RIKEN BioResource Research Center (BRC). On September 28, 2020, we will start distributing these resources as they are ready to be shipped. For details of each resource, please refer the attached table. These resources will be distributed to not-for-profit academic research conducted by universities and national\/public research institutions at normal fees <strong>without license fee to MBL<\/strong>. In addition, we will distribute these resources to commercial entities and to for-profit-use at non-profit organizations after these users obtain an approval from MBL in advance. <\/p>\n<p>These resources include large numbers of functional fluorescent protein resources developed by Dr. Atsushi Miyawaki Laboratory, RIKEN Center for Brain Science (CBS), such as (1) fluorescent proteins with various wavelength characteristics, (2) photoconversion type that changes color by light irradiation, and (3) photochromism type that can control light emission and extinction and can be used for super-resolution imaging. There are also a series of resources that can visualize various subcellular locations.<\/p>\n<p>We are looking forward to receiving many requests for these resources from you. <\/p>\n<p>For questions regarding the fluorescent protein resources, please refer at the attached table, and contact the Gene Engineering Division.<\/p>\n<h3>Ordering <i>CoralHue<\/i>&trade; Fluorescent Protein Resources<\/h3>\n<div class=\"t_left\"><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0067j#order\">in Japanese<img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/jpn.gif\" alt=\"\" \/><\/a>\n<\/div>\n<p><span style=\"text-decoration: underline;\">Terms and Conditions for Distribution<\/span>:<\/p>\n<ol>\n<li>In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR is requested.<\/li>\n<li>A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.<\/li>\n<li>The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. <\/li>\n<\/ol>\n<p>&nbsp;<br \/>\n\n<table id=\"tablepress-21\" class=\"tablepress tablepress-id-21\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><u>Distribution fee per clone<\/u>:<br \/>Please refer <a href=\"https:\/\/dna.brc.riken.jp\/en\/orderingen\/order_feeen\">Fee and Payment<\/a><ul><li>The cost of shipping containers, dry ice and freight will be charged separately. These are not included in the fee.<\/li><li>For details for fee and payment, please visit <a href=\"\/en\/orderingen\">Information of Request for Distribution<\/a>.<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-21 from cache --><br \/>\n&nbsp;<\/p>\n<h4>Order Forms<\/h4>\n<p>Order form and Material Transfer Agreement (MTA) are available at individual catalog page of the plasmids.<\/p>\n<h4>Address for orders<\/h4>\n<p>Please complete one copy of Form A and two copies of Form C and send them to the Gene Engineering Division by post or e-mail.<br \/>\nWe look forward to receiving your order.<br \/>\n\n<table id=\"tablepress-1\" class=\"tablepress tablepress-id-1\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\">The DNA Bank, RIKEN BioResource Research Center (BRC),<br \/>\n3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan<br \/>\n E-mail: &#100;&#110;&#97;&#95;&#115;&#101;&#99;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-1 from cache --><br \/>\n&nbsp;<\/p>\n<h4>Payment and charges<\/h4>\n<p>An INVOICE will be sent to you once you have received the requested materials.<br \/>\nPlease refer to the following page for more details: <a href=\"\/en\/orderingen\">Information of Request for Distribution<\/a><br \/>\nRIKEN is a non-profit organization and therefore requires all applicants to cover all bank charges, (including exchange, commission and handling fees) incurred in sending payment for resources they have ordered.<br \/>\n<b>To contact us<\/b>:<br \/>\n\n<table id=\"tablepress-1-no-2\" class=\"tablepress tablepress-id-1\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\">The DNA Bank, RIKEN BioResource Research Center (BRC),<br \/>\n3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan<br \/>\n E-mail: &#100;&#110;&#97;&#95;&#115;&#101;&#99;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/p>\n<h4>Shipping<\/h4>\n<p><span style=\"text-decoration: underline;\">Form<\/span>:<br \/>\nDNA solution with TE buffer (approx. 1 microgram) per clone.<br \/>\n&nbsp;<br \/>\n<span style=\"text-decoration: underline;\">Remarks<\/span>:<br \/>\nIf you have any questions regarding our DNA resources or related matters, please feel free to contact the Gene Engineering Division.<\/p>\n<p><a href=\"#top\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/images\/goup.gif\" alt=\"\" \/>go to top<\/a><\/p>\n<h3><i>CoralHue<\/i>&trade; Basic Fluorescent Proteins<\/h3>\n<table>\n<tbody>\n<tr>\n<td colspan=\"9\" style=\"background-color:powderblue;\" \/*#B4F4FF*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Kusabira-Cyan<\/span><br \/>\nKikuchi A et al. Crystal structure of a new cyan fluorescent protein and its hue-shifted variants. Biochemistry 48, 5276-83 (2009) (PMID: 19402703)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>KCy1<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>453<\/a><\/td>\n<td>486<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18310\">RDB18310<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background-color:paleturquoise;\" \/*#C4FFF8*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Midoriishi-Cyan<\/span><br \/>\nKarasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor\/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>MiCy1<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>472<\/a><\/td>\n<td>495<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18265\">RDB18265<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>mMiCy1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>470<\/a><\/td>\n<td>496<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18289\">RDB18289<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hmMiCy1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>470<\/a><\/td>\n<td>496<\/a><\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18290\">RDB18290<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18291\">RDB18291<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18292\">RDB18292<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18288\">RDB18288<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background-color:palegreen;\" \/*#C8EBDA*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Umikinoko-Green<\/span><br \/>\nTsutsui H et al. Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods. 5, 683-685 (2008) (PMID: 18622396)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>mUKG1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>483<\/a><\/td>\n<td>499<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18306\">RDB18306<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hmUKG1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>483<\/a><\/td>\n<td>499<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18307\">RDB18307<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18308\">RDB18308<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18309\">RDB18309<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background-color:lightgreen;\" \/*#BBECBE*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Azami-Green<\/span><br \/>\nKarasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>AG<\/a><\/td>\n<td>Tetramer<\/a><\/td>\n<td>492<\/a><\/td>\n<td>505<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18244\">RDB18244<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>mAG1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>492<\/a><\/td>\n<td>505<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18246\">RDB18246<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18247\">RDB18247<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18248\">RDB18248<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hmAG1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>492<\/a><\/td>\n<td>505<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18249\">RDB18249<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18250\">RDB18250<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18251\">RDB18251<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18252\">RDB18252<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18245\">RDB18245<\/a><\/td>\n<\/tr>\n<tr>\n<td>hmAG407<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>407<\/a><\/td>\n<td>498<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18328\">RDB18328<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background-color:peachpuff;\" \/*#F9D5BB*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Kusabira-Orange<\/span><br \/>\nKarasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor\/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>KO1<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>548<\/a><\/td>\n<td>561<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18253\">RDB18253<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>mKO1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>548<\/a><\/td>\n<td>559<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18258\">RDB18258<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18259\">RDB18259<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18260\">RDB18260<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>mKO2<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>551<\/a><\/td>\n<td>565<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18299\">RDB18299<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hKO1<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>548<\/a><\/td>\n<td>561<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18254\">RDB18254<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18255\">RDB18255<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18256\">RDB18256<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hmKO1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>548<\/a><\/td>\n<td>559<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18261\">RDB18261<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18262\">RDB18262<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18263\">RDB18263<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18264\">RDB18264<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18257\">RDB18257<\/a><\/td>\n<\/tr>\n<tr>\n<td>hmKO2<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>551<\/a><\/td>\n<td>565<\/a><\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18300\">RDB18300<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18301\">RDB18301<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18302\">RDB18302<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18298\">RDB18298<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background-color:lightpink;\" \/*#FAC5C8*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Keima570<\/span><br \/>\nKogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>dKeima570<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>440<\/a><\/td>\n<td>570<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18294\">RDB18294<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hdKeima570<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>440<\/a><\/td>\n<td>570<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18295\">RDB18295<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18296\">RDB18296<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18293\">RDB18293<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background-color:lightcoral;\" \/*#F5A9A9*\/ ><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Keima-Red<\/span><br \/>\nKogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>dKeima-Red<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>440<\/a><\/td>\n<td>616<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18285\">RDB18285<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>mKeima-Red<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>440<\/a><\/td>\n<td>620<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18280\">RDB18280<\/a><\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18281\">RDB18281<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hdKeima-Red<\/a><\/td>\n<td>Dimer<\/a><\/td>\n<td>440<\/a><\/td>\n<td>616<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18286\">RDB18286<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18287\">RDB18287<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18284\">RDB18284<\/a><\/td>\n<\/tr>\n<tr>\n<td>hmKeima-Red<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>440<\/a><\/td>\n<td>620<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18282\">RDB18282<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18283\">RDB18283<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18279\">RDB18279<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background:linear-gradient(to right, lightgreen, lightcoral)\"><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Kaede (photoconversion type)<\/span><br \/>\nAndo R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>Kaede<\/a><\/td>\n<td>Tetramer<\/a><\/td>\n<td>508\/572<\/a><\/td>\n<td>518\/580<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18241\">RDB18241<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18242\">RDB18242<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18243\">RDB18243<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background:linear-gradient(to right, lightgreen, lightcoral)\"><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Kikume Green-Red (photoconversion type)<\/span><br \/>\nHabuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591)<br \/>\nTsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>KikGR1<\/a><\/td>\n<td>Tetramer<\/a><\/td>\n<td>507\/583<\/a><\/td>\n<td>517\/593<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18272\">RDB18272<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18273\">RDB18273<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18274\">RDB18274<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>mKikGR1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>505\/580<\/a><\/td>\n<td>517\/591<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18304\">RDB18304<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hKikGR1<\/a><\/td>\n<td>Tetramer<\/a><\/td>\n<td>507\/583<\/a><\/td>\n<td>517\/593<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18275\">RDB18275<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18276\">RDB18276<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18277\">RDB18277<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18278\">RDB18278<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18271\">RDB18271<\/a><\/td>\n<\/tr>\n<tr>\n<td>hmKikGR1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>505\/580<\/a><\/td>\n<td>517\/591<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18305\">RDB18305<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18303\">RDB18303<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" class=\"t0\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"9\" style=\"background:linear-gradient(to right, lightgreen, dimgray)\"><span style=\"color: mediumblue; font-weight:bold; font-size:large\">Dronpa Green (photochromism type)<\/span><br \/>\nAndo R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670)<\/td>\n<\/tr>\n<tr>\n<th>Abbr.<\/a><\/th>\n<th>Form<\/a><\/th>\n<th>Excitation maxima (nm)<\/a><\/th>\n<th>Emission maxima (nm)<\/a><\/th>\n<th>S1 vector<sup>#<\/sup><\/a><\/th>\n<th>MC1 vector<sup>$<\/sup><\/a><\/th>\n<th>MN1 vector<sup>$<\/sup><\/a><\/th>\n<th>MCLinker vector<sup>*<\/sup><\/a><\/th>\n<th>MNLinker vector<sup>*<\/sup><\/th>\n<\/tr>\n<tr>\n<td>DG1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>503<\/a><\/td>\n<td>518<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18267\">RDB18267<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18268\">RDB18268<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18269\">RDB18269<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td>hDG1<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>503<\/a><\/td>\n<td>518<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18270\">RDB18270<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18266\">RDB18266<\/a><\/td>\n<\/tr>\n<tr>\n<td>DG3<\/a><\/td>\n<td>Monomer<\/a><\/td>\n<td>491<\/a><\/td>\n<td>514<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18297\">RDB18297<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table>\n<tbody>\n<tr>\n<td class=\"t0\" valign=\"top\">#<\/td>\n<td class=\"t0\">S1 vector: Vectors for subcloning<\/td>\n<\/tr>\n<tr>\n<td class=\"t0\" valign=\"top\">$<\/td>\n<td class=\"t0\">MC1 vector, MN1 vector: Expression vectors<\/td>\n<\/tr>\n<tr>\n<td class=\"t0\" valign=\"top\">*<\/td>\n<td class=\"t0\">MCLinker vector, MNLinker vector: Expression vectors with flexible linker (The linker sequence: TGNSADGGGGSGGSGGSGGGSTQG)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Organelle Targeting Vector Resources<\/h3>\n<p>Visualizing intracellular organs with fluorescent proteins in expression vector.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Abbr.<\/th>\n<th>Plasma membrane<\/th>\n<th>Mitochondria<\/th>\n<th>Endoplasmic reticulum<\/th>\n<th>Nucleoplasm<\/th>\n<th>Beta-actin<\/th>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:paleturquoise;\" \/*#C4FFF8*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">mMiCy1 <sup>(1)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18323\">RDB18323<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:powderblue;\" \/*#B4F4FF*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">hKCy1 <sup>(1)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18325\">RDB18325<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:lightgreen;\" \/*#BBECBE*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">mAG1 <sup>(2)<\/sup><\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18313\">RDB18313<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18311\">RDB18311<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18312\">RDB18312<\/a><\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18314\">RDB18314<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:lightgreen;\" \/*#BBECBE*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">AG <sup>(2)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18315\">RDB18315<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:lightgreen;\" \/*#BBECBE*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">mAG407 <sup>(2)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18326\">RDB18326<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:peachpuff;\" \/*#F9D5BB*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">mKO1 <sup>(3)<\/sup><\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18318\">RDB18318<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18316\">RDB18316<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18317\">RDB18317<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:peachpuff;\" \/*#F9D5BB*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">hmKO1 <sup>(3)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18319\">RDB18319<\/a><\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:peachpuff;\" \/*#F9D5BB*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">KO1 <sup>(3)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18320\">RDB18320<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:lightpink;\" \/*#FAC5C8*\/><span style=\"color: mediumblue; font-weight:bold; font-size:large\">hdKeima570 <sup>(1)<\/sup><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18327\">RDB18327<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:lightcoral;\" \/*#F5A9A9*\/ ><span style=\"color: mediumblue; font-weight:bold; font-size:large\">mKeima-Red <sup>(1)<\/sup><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18322\">RDB18322<\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td colspan=\"1\" style=\"background-color:lightcoral;\" \/*#F5A9A9*\/ ><span style=\"color: mediumblue; font-weight:bold; font-size:large\">hdKeima-Red <sup>(1)<\/sup><\/td>\n<td><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18324\">RDB18324<\/a><\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>References<\/p>\n<table>\n<tbody>\n<tr>\n<td class=\"t0\" valign=\"top\">(1)<\/td>\n<td class=\"t0\">Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)<\/td>\n<\/tr>\n<tr>\n<td class=\"t0\" valign=\"top\">(2)<\/td>\n<td class=\"t0\">Karasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)<\/td>\n<\/tr>\n<tr>\n<td class=\"t0\" valign=\"top\">(3)<\/td>\n<td class=\"t0\">Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor\/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"#top\">go to top<\/a><\/p>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.06 (T.M. GRP0067e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Distribution of CoralHue&trade; Fluorescent Protein Resources With the generosity of Medical &#038; Biological Laboratories Co., Ltd. (MBL), 88 items of CoralHue&trade; fluorescent protein resources were deposited to RIKEN BioResource Research Center (BRC). On September 28, 2020, we will start distributing these resources as they are ready to be shipped. For details of each resource, please refer the attached table. These resources will be distributed to not-for-profit academic research conducted by universities and national\/public research institutions at normal fees without license fee to MBL. In addition, we will distribute these resources to commercial entities and to for-profit-use at non-profit organizations after these users obtain an approval from MBL in advance. These resources include large numbers of functional fluorescent protein resources developed by Dr. Atsushi Miyawaki Laboratory, RIKEN Center for Brain Science (CBS), such as (1) fluorescent proteins with various wavelength characteristics, (2) photoconversion type that changes color by light irradiation, and (3) photochromism type that can control light emission and extinction and can be used for super-resolution imaging. There are also a series of resources that can visualize various subcellular locations. We are looking forward to receiving many requests for these resources from you. For questions regarding the fluorescent protein resources, [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8918,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8921","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8921"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8921\/revisions"}],"predecessor-version":[{"id":8925,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8921\/revisions\/8925"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8918"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}