{"id":8788,"date":"2025-05-05T09:13:11","date_gmt":"2025-05-05T00:13:11","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8788"},"modified":"2026-01-06T09:02:57","modified_gmt":"2026-01-06T00:02:57","slug":"nbrpcionaen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/clonedcdnaen\/nbrpcionaen","title":{"rendered":"Ciona intestinalis EST Clone"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>General Information<\/h2>\n<p><i>Ciona intestinalis<\/i> EST Clones, known as the &#8220;<i>Ciona intestinalis<\/i> Gene Collection Release I&#8221; (Satou, Y. et al., 2002, Genesis 33 (4): 153-154; PMID, 12203911), were deposited by Dr. Noriyuki Satoh, Facuity of Science Graduate School of Science, Kyoto University.<br \/>\nIn order to access wild type and transgenic animals, please visit <a href=\"https:\/\/marinebio.nbrp.jp\/marinebio\/\">NBRP Ciona intestinalis HP<\/a>.<\/p>\n<p><a name=\"order\"><\/a><\/p>\n<h2>Ordering <i>Ciona intestinalis<\/i> EST Clones<\/h2>\n<div class=\"t_right\"><a href=\"\/ja\/clonedcdnaja\/nbrpcionaja\">in Japanese<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/jpn.gif\" border=\"0\" \/><\/a>\n<\/div>\n<h3 class=\"purple\">Clone search<\/h3>\n<p>Please find ID of individual clone(s) using the following sites.<img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/finder01.gif\" border=\"0\" \/><\/p>\n<ul>\n<li><a href=\"https:\/\/ghost.zool.kyoto-u.ac.jp\/\">Ghost Database<\/a><\/li>\n<li><a href=\"https:\/\/aniseed.fr\/\">ANISEED Database<\/a><\/li>\n<\/ul>\n<div class=\"img_center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/arrow_down.png\" border=\"0\" \/>\n<\/div>\n<h3 class=\"purple\">Order Forms and Material Transfer Agreement (MTA)<\/h3>\n<div>\n<p>\n<table id=\"tablepress-13\" class=\"tablepress tablepress-id-13\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\">Order Form:<br \/>Please complete the form with your shipping information including your account number of an international courier<br \/>(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).<br \/>See detail in <a href=\"\/en\/orderingen\" target=\"_blank\" rel=\"noopener noreferrer\">Information of Request for Distribution<\/a><br \/><br \/>Note:<br \/>The resident of the European Economic Area (EEA) and China, please read <a href=\"\/en\/orderingen#sdirfc\">Special distribution Information to Residents of the Foreign Countries<\/a><\/td>\n<\/tr>\n<tr class=\"row-2\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmeacrd\">Order Form for Credit Card Payment. (Visa or Master Card only) [Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmeexord\">Example of order form<\/a>]<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmead\">Order Form for Bank Transfer Payment. [Word]<\/a>&nbsp;[<a href=\"https:\/\/brc.riken.jp\/dna\/frmeexord\">Example of order form<\/a>]<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-13 from cache --><br \/>\n\n<table id=\"tablepress-12\" class=\"tablepress tablepress-id-12\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><a href=\"https:\/\/brc.riken.jp\/dna\/frmecad\">Material Transfer Agreement (Category I MTA) [Word]<\/a><br \/><br \/>\nFor the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.<br \/><ul><li>Regarding Section 2(a):<br \/>Please write your research purpose in detail. We need description specifically how and for what purpose you are going to use the DNA Bank resource(s). If the information is considered insufficient, we may ask you to add more or rewrite it.<br \/>We can check whether the documents are filled out correctly or not in advance. Please email documents to us.<\/li><li>Regarding signature line:<br \/>\"Authorized Representative\" is a person who is responsible for intellectual property rights. We request that the candidate is in one of the following positions, he\/she can sign the MTA as the Authorized Representative. For anything unclear, please contact us by email.<br \/>\n<ul><li> College\/University\/Graduate School: President, Dean, Director or Head of Department<\/li><li>Research Institution: Director<\/li><li>Corporation: President, CEO, Director<\/li><li>Any officer appointed as Intellectual Property Administrator by the organization<\/li><\/ul>The MTA is a formal contract to be executed between institutions. Therefore, we ask your Authorized Representative to be an authority listed above.<\/li><li>Please ask us if applicable for the following use:<ul><li>For research to be conducted by for-profit organizations.<\/li><li>For collaborative research between for-profit organization and not-for-profit organization.<\/li><li>For research by not-for- organization outsourced and sponsored by for-profit organization.<\/li><li>For for-profit research by not-for-profit organization including R&amp;D with the aim of patent acquisition.<\/li><\/ul><\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-12 from cache --><\/p>\n<\/div>\n<div class=\"img_center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/arrow_down.png\" border=\"0\" \/>\n<\/div>\n<p><span style=\"text-decoration: underline;\">Terms and Conditions for Distribution<\/span><\/p>\n<ol>\n<li>In publishing their resuits in any kind of publication types, RECIPIENT refers to the Ghost Home ( genomic and cDNA resources ) web site<br \/>\n(https:\/\/ghost.zool.kyoto-u.ac.jp\/) and DEPOSITOR&#8217;s related publications,<\/li>\n<li>Sending the DEPOSITOR a reprint or equivalent of RECIPIENT&#8217;s publication after publishing their resuits,<\/li>\n<li>Using the clones only for academic purpose, and<\/li>\n<li>When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT&#8217;s name and their affiliation.<\/li>\n<\/ol>\n<p>\n&nbsp;&nbsp;&nbsp;Uses other than for academic research by non-profit institutions:<br \/>\n&nbsp;&nbsp;&nbsp;Please contact the Gene Engineering Division.<\/p>\n<p><!-- Begin of Fee Menu --><br \/>\n\n<table id=\"tablepress-20\" class=\"tablepress tablepress-id-20\">\n<tbody>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><u>Distribution fee per clone<\/u>:<br \/>Please refer <a href=\"https:\/\/dna.brc.riken.jp\/en\/orderingen\/order_feeen\">Fee and Payment<\/a><ul><li>The cost of shipping containers, dry ice and freight will be charged separately. These are not included in the fee.<\/li><li>For details for fee and payment, please visit <a href=\"\/en\/orderingen\">Information of Request for Distribution<\/a>.<\/li><\/ul><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-20 from cache --><\/p>\n<p><!-- End of Fee Menu --><\/p>\n<p><span style=\"text-decoration: underline;\">Form<\/span>:<br \/>\nDNA solution with TE buffer (approx. 1 microgram) per clone.<\/p>\n<p><span style=\"text-decoration: underline;\">Remarks<\/span>:<br \/>\n&diams; Entire DNA sequence of cDNA clone is checked prior to delivery.<\/p>\n<p>If you have any questions regarding our DNA resources or related matters, please feel free to contact the Gene Engineering Division.<\/p>\n<div class=\"img_center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/arrow_down.png\" border=\"0\" \/>\n<\/div>\n<h3 class=\"purple\">Address for orders<\/h3>\n<p>Please complete one copy of Form A and Form C and send them to the Gene Engineering Division by e-mail or post. In case sending them by e-mail, please send us the originals by post afterwards.<br \/>\nWe look forward to receiving your order.<br \/>\n\n<table id=\"tablepress-1\" class=\"tablepress tablepress-id-1\">\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-1\">\n\t<td class=\"column-1\">The DNA Bank, RIKEN BioResource Research Center (BRC),<br \/>\n3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan<br \/>\n E-mail: &#100;&#110;&#97;&#95;&#115;&#101;&#99;&#46;&#98;&#114;&#99;&#64;&#114;&#105;&#107;&#101;&#110;&#46;&#106;&#112;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-1 from cache --><\/p>\n<div class=\"img_center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/arrow_down.png\" border=\"0\" \/>\n<\/div>\n<h3 class=\"purple\">Payment and charges<\/h3>\n<p>An INVOICE will be sent to you once you have received the requested materials.<br \/>\nPlease refer to the following page for more details:<br \/>\n<a href=\"\/en\/orderingen\">Information of Request for Distribution<\/a>.<br \/>\nRIKEN is a non-profit organization and therefore requires all applicants to cover all bank charges, (including exchange, commission and handling fees) incurred in sending payment for resources they have ordered.<\/p>\n<hr \/>\n<p><a name=\"information\"><\/a><\/p>\n<h2 class=\"purple\">Information<\/h2>\n<p><span style=\"text-decoration: underline;\">Library information:<\/span><\/p>\n<table>\n<tbody>\n<tr>\n<th width=\"14%\">Library ID<\/th>\n<th width=\"21%\">Library<\/th>\n<th width=\"30%\">Clone ID<\/th>\n<th width=\"21%\">vector<\/th>\n<\/tr>\n<tr>\n<td>CiEG<\/td>\n<td>Egg<\/td>\n<td>cieg001a01-cieg101p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiCL<\/td>\n<td>Cleaving embryo<\/td>\n<td>cicl001a01-cicl022p24<br \/>\ncicl024a01-cicl105p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiGN<\/td>\n<td>Gastruia\/Neuruia<\/td>\n<td>cign001a01-cign081p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiTB<\/td>\n<td>Tailbud embryo<\/td>\n<td>citb001a01-citb105p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiLV<\/td>\n<td>Larva<\/td>\n<td>cilv001a01-cilv090p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiAD<\/td>\n<td>Young aduit<\/td>\n<td>ciad001a01-ciad020p24<br \/>\nciad024a01-ciad103p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiGD<\/td>\n<td>Gonad<\/td>\n<td>cigd001a01-cigd049p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiTS<\/td>\n<td>Testis<\/td>\n<td>cits001a01-cits050p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiES<\/td>\n<td>Endostyle<\/td>\n<td>cies001a01-cies009p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiNC<\/td>\n<td>Neural complex<\/td>\n<td>cinc001a01-cinc034p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiHT<\/td>\n<td>Heart<\/td>\n<td>ciht001a01-ciht042p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiBD<\/td>\n<td>Blood cells<\/td>\n<td>cibd001a01-cibd085p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiEM<\/td>\n<td>Embryo mix<\/td>\n<td>ciem801a01-ciem854p24<\/td>\n<td>pDONR222<\/td>\n<\/tr>\n<tr>\n<td>CiJV<\/td>\n<td>Juvenile<\/td>\n<td>cijv001a01-cijv051p24<br \/>\ncijv201a01-cijv227p24<br \/>\ncijv401a01-cijv430p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiDG<\/td>\n<td>Digestive Gland<\/td>\n<td>cidg001a01-cidg007p24<br \/>\ncidg801a01-cidg851p24<\/td>\n<td>pDONR222<\/td>\n<\/tr>\n<tr>\n<td>CiMA<\/td>\n<td>Mature aduit<\/td>\n<td>cima001a01-cima041p24<br \/>\ncima044a01-cima056p24<\/td>\n<td>pBluescriptII SK(-)<\/td>\n<\/tr>\n<tr>\n<td>CiMA<\/td>\n<td>Mature aduit<\/td>\n<td>cima801a01-cima854p24<\/td>\n<td>pDONR222<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"text-decoration: underline;\">References:<\/span><br \/>\nThe papers describing these libraries have been published in journals indicated below:<\/p>\n<ol>\n<li>Jeffery, W.R., Chiba, T., Krajka, F.R., Deyts, C., Satoh, N., Joly, J.S., 2008, Trunk lateral cells are neural crest-like cells in the ascidian Ciona intestinalis: insights into the ancestry and evolution of the neural crest. Dev. Biol., 324 (1): 152-160. PMID: 18801357.<\/li>\n<li>Imai, K.S., Hino, K., Yagi, K., Satoh, N., Satou, Y., 2004, Gene expression profiles of transcription factors and signaling molecuies in the ascidian embryo: towards a comprehensive understanding of gene networks. Development, 131 (16): 4047-4058. PMID: 15269171.<\/li>\n<li>Azumi, K., Takahashi, H., Miki, Y., Fujie, M., Usami, T., Ishikawa, H., Kitayama, A., Satou, Y., Ueno, N., Satoh, N., 2003, Construction of a cDNA microarray derived from the ascidian Ciona intestinalis. Zoolog. Sci. 20 (10): 1223-1229. PMID: 14569145.<\/li>\n<li>Satou, Y., Yamada, L., Mochizuki, Y., Takatori, N., Kawashima, T., Sasaki, A., Hamaguchi, M., Awazu, S., Yagi, K., Sasakura, Y., Nakayama, A., Ishikawa, H., Inaba, K., Satoh, N., 2002, A cDNA resource from the basal chordate Ciona intestinalis. Genesis, 33 (4): 153-154. PMID: 12203911.<\/li>\n<li><a href=\"https:\/\/www.cc.kochi-u.ac.jp\/~tatataa\/index2.html\">Embryonic retinoic acid target genes in the ascidian Ciona intestinalis<\/a> web site<\/li>\n<li><a href=\"https:\/\/ghost.zool.kyoto-u.ac.jp\/\">The Ghost Database<\/a> web site<\/li>\n<\/ol>\n<hr \/>\n<p style=\"text-align:left;\">2026.01.04 (GRP0088e T.M.)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>General Information Ciona intestinalis EST Clones, known as the &#8220;Ciona intestinalis Gene Collection Release I&#8221; (Satou, Y. et al., 2002, Genesis 33 (4): 153-154; PMID, 12203911), were deposited by Dr. Noriyuki Satoh, Facuity of Science Graduate School of Science, Kyoto University. In order to access wild type and transgenic animals, please visit NBRP Ciona intestinalis HP. Ordering Ciona intestinalis EST Clones in Japanese Clone search Please find ID of individual clone(s) using the following sites. Ghost Database ANISEED Database Order Forms and Material Transfer Agreement (MTA) Terms and Conditions for Distribution In publishing their resuits in any kind of publication types, RECIPIENT refers to the Ghost Home ( genomic and cDNA resources ) web site (https:\/\/ghost.zool.kyoto-u.ac.jp\/) and DEPOSITOR&#8217;s related publications, Sending the DEPOSITOR a reprint or equivalent of RECIPIENT&#8217;s publication after publishing their resuits, Using the clones only for academic purpose, and When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT&#8217;s name and their affiliation. &nbsp;&nbsp;&nbsp;Uses other than for academic research by non-profit institutions: &nbsp;&nbsp;&nbsp;Please contact the Gene Engineering Division. Form: DNA solution with TE buffer (approx. 1 microgram) per clone. Remarks: &diams; Entire DNA sequence of cDNA clone is checked prior to delivery. If you [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8757,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8788","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8788","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8788"}],"version-history":[{"count":4,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8788\/revisions"}],"predecessor-version":[{"id":9090,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8788\/revisions\/9090"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8757"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}