{"id":8752,"date":"2025-05-05T09:00:38","date_gmt":"2025-05-05T00:00:38","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8752"},"modified":"2025-06-02T08:13:08","modified_gmt":"2025-06-01T23:13:08","slug":"suicedegeneen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/researchen\/suicedegeneen","title":{"rendered":"suicedegeneen"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Suicide gene<\/h2>\n<h3>Diphtheria toxin (DT-A)<\/h3>\n<table border=\"1\">\n<tbody>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB10550\">RDB10550<\/a><\/td>\n<td>pBRblue-MC1DTApA<\/td>\n<td>Expression clone of diphtheria toxin (DT-A)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h3>HSV thymidine kinase (HSV-Tk)<\/h3>\n<p>Also refer <a href=\"https:\/\/dnaconda.riken.jp\/search\/KO\/M00\/M00002.html\">HSV-TK<\/a><\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19575\">RDB19575<\/a><\/td>\n<td>pUlrTK<\/td>\n<td>Genome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, &amp; 5&#8217;UAS<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19569\">RDB19569<\/a><\/td>\n<td>pUCtk2x2<\/td>\n<td>Universal donor vector for double-tk CRISPR\/Cas9 knock-in system. Kpn I\/Asp718I site is available for insertion of homology arm<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18670\">RDB18670<\/a><\/td>\n<td>pCRtk2x2NN<\/td>\n<td>Backbone vector for constructing donor vector with double HSV-thymidine kinase.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17468\">RDB17468<\/a><\/td>\n<td>pUC tk2<\/td>\n<td>Backbone vector for constructing donor vector with single HSV-thymidine kinase.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01027\">RDB01027<\/a><\/td>\n<td>pTK5<\/td>\n<td>HSV I thymidine kinase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01427\">RDB01427<\/a><\/td>\n<td>pCA HSVtK<\/td>\n<td>pCAcctk Simplex herpes virus; tk gene expression<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01692\">RDB01692<\/a><\/td>\n<td>pCM-TK<\/td>\n<td>CM-HSV-TK genomic DNA<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01465\">RDB01465<\/a><\/td>\n<td>pMFG HSVtk<\/td>\n<td>Recombinant retrovirus vector; HSV tk gene cDNA expression<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02050\">RDB02050<\/a><\/td>\n<td>AxCAUT<\/td>\n<td>E. coli + HSV uracil phosphoribosyl transferase (UPRTase) and HSV thymidine kinase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02203\">RDB02203<\/a><\/td>\n<td>AxCMTK\/R<\/td>\n<td>Recombinant adenovirus expressing HSV tk regulated by CM promoter.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02206\">RDB02206<\/a><\/td>\n<td>AxCAUT AseI(-)<\/td>\n<td>Expressing E. coli uracil phosphoribosyl transferase (UPRTase) and HSV tk regulated by CAG promoter.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02211\">RDB02211<\/a><\/td>\n<td>AxCAUP-F\/RGD<\/td>\n<td>Expressing E. coli uracil phosphoribosyl transferase (UPRTase) regulated by CAG promoter. Fiber-mutant, F\/RGD.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02213\">RDB02213<\/a><\/td>\n<td>AxCAUT-F\/RGD<\/td>\n<td>Expressing E. coli uracil phosphoribosyl transferase (UPRTase) and HSV tk. Fiber-mutant, F\/RGD.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02216\">RDB02216<\/a><\/td>\n<td>AxCMTKR-F\/RGD<\/td>\n<td>Recombinant adenovirus expressing HSV thymidine kinase.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02218\">RDB02218<\/a><\/td>\n<td>AxCMTKR-F\/gsMSH beta<\/td>\n<td>Recombinant adenovirus expressing HSV thymidine kinase.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01431\">RDB01431<\/a><\/td>\n<td>AxPGK-HSVtK<\/td>\n<td>Recombinant adenovirus expressing simplex herpes virus tk gene<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01429\">RDB01429<\/a><\/td>\n<td>AxCAHSVtk<\/td>\n<td>Recombinant adenovirus expressing herpes simplex virus tk gene<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01428\">RDB01428<\/a><\/td>\n<td>pAxCA HSVtK<\/td>\n<td>Simplex herpes virus; tk gene expression; Adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01430\">RDB01430<\/a><\/td>\n<td>pAxPGK HSVtK<\/td>\n<td>Simplex herpes virus; tk gene expression; Adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02028\">RDB02028<\/a><\/td>\n<td>pAxE1(AdB)-CdUT<\/td>\n<td>E. coli + HSV uracil phosphoribosyl transferase (UPRTase) and HSV thymidine kinase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02030\">RDB02030<\/a><\/td>\n<td>pAxE1CAUT<\/td>\n<td>E. coli + HSV uracil phosphoribosyl transferase (UPRTase) and HSV thymidine kinase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02051\">RDB02051<\/a><\/td>\n<td>pAxCAUT<\/td>\n<td>E. coli + HSV uracil phosphoribosyl transferase (UPRTase) and HSV thymidine kinase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01903\">RDB01903<\/a><\/td>\n<td>pCAUT<\/td>\n<td>E. coli UPRTase + HSV-TK, genomic DNA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Cytosine deaminase (CD)<\/h3>\n<table border=\"1\">\n<tbody>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01591\">RDB01591<\/a><\/td>\n<td>pAxCACD<\/td>\n<td>E. coli cytosine deaminase gene expression; Adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01592\">RDB01592<\/a><\/td>\n<td>pAxCEAprCD<\/td>\n<td>E. coli cytosine deaminase gene expression; Adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01714\">RDB01714<\/a><\/td>\n<td>pAx1-CM-CD<\/td>\n<td>E. coli CM-CD, genomic DNA, adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01725\">RDB01725<\/a><\/td>\n<td>pAx1-CEACD-CAUP<\/td>\n<td>E. coli Cytosine deaminase (CD), uracil phospho-ribosyltransferase (UP), adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01696\">RDB01696<\/a><\/td>\n<td>pCM-CD<\/td>\n<td>E. coli Cytosine deaminase (CD)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>Uracil phosphoribosyl transferase (UPRTase)<\/h3>\n<table border=\"1\">\n<tbody>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01743\">RDB01743<\/a><\/td>\n<td>Ax1 CAUP<\/td>\n<td>E.coli UPRT ase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02044\">RDB02044<\/a><\/td>\n<td>AxPSUP<\/td>\n<td>Expressing E. coli UPRT age regulated by human PSA promoter.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02050\">RDB02050<\/a><\/td>\n<td>AxCAUT<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02206\">RDB02206<\/a><\/td>\n<td>AxCAUT AseI(-)<\/td>\n<td>Expressing E. coli UPRTase and HSV tk regulated by CAG promoter.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02207\">RDB02207<\/a><\/td>\n<td>AxCAUP AseI(-)<\/td>\n<td>Expressing E. coli UPRTase regulated by CAG promoter.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02213\">RDB02213<\/a><\/td>\n<td>AxCAUT-F\/RGD<\/td>\n<td>Expressing E. coli UPRTase and HSV tk. Fiber-mutant, F\/RGD.<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01721\">RDB01721<\/a><\/td>\n<td>pAx1-CAUP<\/td>\n<td>E. coli urasil phosphoribosyl transferase (UP) gene, adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01722\">RDB01722<\/a><\/td>\n<td>pAx1-CEAZ-CAUP<\/td>\n<td>E. coli lacZ gene and urasil phosphoribosyl transferase (UP) gene, adenovirus vector<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01725\">RDB01725<\/a><\/td>\n<td>pAx1-CEACD-CAUP<\/td>\n<td>E. coli Cytosine deaminase (CD) and uracil phosphoribosyl transferase (UPRTase)<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02028\">RDB02028<\/a><\/td>\n<td>pAxE1(AdB)-CdUT<\/td>\n<td>UPRTase, HSVtk<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02030\">RDB02030<\/a><\/td>\n<td>pAxE1CAUT<\/td>\n<td>UPRTase, HSVtk<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02032\">RDB02032<\/a><\/td>\n<td>pAxE1CAUP<\/td>\n<td>Uracil phosphoribosyl transferase (UPRTase)<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02045\">RDB02045<\/a><\/td>\n<td>pAxPSUP<\/td>\n<td>Shuttle vector to generate recombinant adenovirus expressing E. coli UPRTase<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02051\">RDB02051<\/a><\/td>\n<td>pAxCAUT<\/td>\n<td>UPRTase, HSVtk<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01701\">RDB01701<\/a><\/td>\n<td>pSK II+UP<\/td>\n<td>pSK II+ upp, E. coli phosphoribosyl transferaze genomic DNA<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01879\">RDB01879<\/a><\/td>\n<td>pCEA CD CAUP<\/td>\n<td>Expression vector of E. coli Cytosine deaminase (CD) and uracil phosphoribosyl transferase (UPRTase)<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01901\">RDB01901<\/a><\/td>\n<td>pPSUP<\/td>\n<td>Human PSA promoter + E. coli UPRTase genomic DNA<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01903\">RDB01903<\/a><\/td>\n<td>pCAUT<\/td>\n<td>E. coli UPRTase + HSV-TK, genomic DNA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div align=\"Center\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/GSB\/suicide.png\" border=\"0\" \/>\n<\/div>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.31 (T.M.)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Suicide gene Diphtheria toxin (DT-A) RDB10550 pBRblue-MC1DTApA Expression clone of diphtheria toxin (DT-A) HSV thymidine kinase (HSV-Tk) Also refer HSV-TK RDB19575 pUlrTK Genome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, &amp; 5&#8217;UAS RDB19569 pUCtk2x2 Universal donor vector for double-tk CRISPR\/Cas9 knock-in system. Kpn I\/Asp718I site is available for insertion of homology arm RDB18670 pCRtk2x2NN Backbone vector for constructing donor vector with double HSV-thymidine kinase. RDB17468 pUC tk2 Backbone vector for constructing donor vector with single HSV-thymidine kinase. RDB01027 pTK5 HSV I thymidine kinase RDB01427 pCA HSVtK pCAcctk Simplex herpes virus; tk gene expression RDB01692 pCM-TK CM-HSV-TK genomic DNA RDB01465 pMFG HSVtk Recombinant retrovirus vector; HSV tk gene cDNA expression RDB02050 AxCAUT E. coli + HSV uracil phosphoribosyl transferase (UPRTase) and HSV thymidine kinase RDB02203 AxCMTK\/R Recombinant adenovirus expressing HSV tk regulated by CM promoter. RDB02206 AxCAUT AseI(-) Expressing E. coli uracil phosphoribosyl transferase (UPRTase) and HSV tk regulated by CAG promoter. RDB02211 AxCAUP-F\/RGD Expressing E. coli uracil phosphoribosyl transferase (UPRTase) regulated by CAG promoter. Fiber-mutant, F\/RGD. RDB02213 AxCAUT-F\/RGD Expressing E. coli uracil phosphoribosyl transferase (UPRTase) and HSV tk. Fiber-mutant, F\/RGD. RDB02216 AxCMTKR-F\/RGD Recombinant adenovirus expressing HSV thymidine kinase. [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8163,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8752","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8752","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8752"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8752\/revisions"}],"predecessor-version":[{"id":8997,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8752\/revisions\/8997"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8163"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8752"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}