{"id":8709,"date":"2025-05-03T16:35:12","date_gmt":"2025-05-03T07:35:12","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8709"},"modified":"2025-05-03T16:35:48","modified_gmt":"2025-05-03T07:35:48","slug":"cerevisiaeexpen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/cloningen\/cerevisiaeexpen","title":{"rendered":"Expression Vector Backbone of <i>Saccharomyces cerevisiae<\/i>"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h4>Vectors with fluorescent protein marker<script>newImage(2022,02,03)<\/script><\/h4>\n<p style=\"text-decoration: underline;\">Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells.<\/p>\n<p>Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y.<br \/>\nFront. Cell Dev. Biol. 8: 571388 (2020). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33330450\">PubMed PMID 33330450<\/a>.<br \/>\n(including unpublished bioresource)<\/p>\n<table width=\"800\" border=\"1\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name<\/th>\n<th>Running title<\/th>\n<\/tr>\n<tr>\n<td>RDB19364<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19364.html\">pYU68 pRS313-ADH1p-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. HIS3 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19365<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19365.html\">pYU69 pRS314-ADH1p-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. TRP1 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19366<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19366.html\">pYU70 pRS315-ADH1p-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. LEU2 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19367<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19367.html\">pYU71 pRS316-ADH1p-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19368<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19368.html\">pYU72 pRS313-ADH1p-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. HIS3 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19369<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19369.html\">pYU73 pRS314-ADH1p-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. TRP1 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19370<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19370.html\">pYU74 pRS315-ADH1p-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. LEU2 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19371<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19371.html\">pYU75 pRS316-ADH1p-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19372<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19372.html\">pYU76 pRS313-GPDp-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. HIS3 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19373<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19373.html\">pYU77 pRS314-GPDp-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. TRP1 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19374<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19374.html\">pYU78 pRS315-GPDp-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. LEU2 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19375<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19375.html\">pYU79 pRS316-GPDp-MCS-GFP(S65T)-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. URA3 marker. GFP(S65T)-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19376<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19376.html\">pYU80 pRS313-GPDp-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. HIS3 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19377<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19377.html\">pYU81 pRS314-GPDp-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. TRP1 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19378<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19378.html\">pYU82 pRS315-GPDp-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. LEU2 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19379<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19379.html\">pYU83 pRS316-GPDp-MCS-mCherry-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at C-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19380<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19380.html\">pYU84 pRS313-GPDp-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. HIS3 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19381<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19381.html\">pYU85 pRS314-GPDp-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. TRP1 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19382<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19382.html\">pYU86 pRS315-GPDp-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. LEU2 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19383<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19383.html\">pYU87 pRS316-GPDp-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. URA3 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19384<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19384.html\">pYU88 pRS313-ADH1p-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. HIS3 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19385<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19385.html\">pYU89 pRS314-ADH1p-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. TRP1 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19386<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19386.html\">pYU90 pRS315-ADH1p-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. LEU2 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19387<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19387.html\">pYU91 pRS316-ADH1p-GFP(S65T)-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. GFP(S65T)-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19388<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19388.html\">pYU92 pRS313-GPDp-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. HIS3 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19389<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19389.html\">pYU93 pRS314-GPDp-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. TRP1 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19390<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19390.html\">pYU94 pRS315-GPDp-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. LEU2 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19391<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19391.html\">pYU95 pRS316-GPDp-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the GPD1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19392<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19392.html\">pYU96 pRS313-ADH1p-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. HIS3 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19393<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19393.html\">pYU97 pRS314-ADH1p-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. TRP1 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19394<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19394.html\">pYU98 pRS315-ADH1p-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. LEU2 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<tr>\n<td>RDB19395<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB19\/RDB19395.html\">pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter<\/a><\/td>\n<td>Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus. <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>CRITGI, a new tool to control gene induction in budding yeast<\/h4>\n<p style=\"text-decoration: underline;\">CRISPR\/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner.<\/p>\n<p>Hanasaki M, Masumoto H.<br \/>\nSci. Rep. 9 (1): 15300 (2019). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31653950\">PubMed PMID 31653950<\/a>.<\/p>\n<table width=\"800\" border=\"1\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name<\/th>\n<th>Running title<\/th>\n<\/tr>\n<tr>\n<td>RDB18135<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18135\">PHM661<\/a><\/td>\n<td>Integration plasmid to Ty1 loci, YIplac128 back bone (pTy1). CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone. <\/td>\n<\/tr>\n<tr>\n<td>RDB18136<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18136\">PHM688<\/a><\/td>\n<td>Integration plasmid to Ty1 loci, pRS403 back bone. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18137<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18137\">PHM663<\/a><\/td>\n<td>Cas9 and sgRNA plasmid helping pTy1 integration into Ty1 loci. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18138<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18138\">PHM664<\/a><\/td>\n<td>Cas9 and sgRNA #4 plasmid helping pTy1 integration into Ty1 loci. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18139<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18139\">PHM740<\/a><\/td>\n<td>Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18140<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18140\">PHM760<\/a><\/td>\n<td>Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18141<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18141\">PHM745<\/a><\/td>\n<td>Integration plasmid with TDH3 promoter to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18142<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18142\">PHM757<\/a><\/td>\n<td>Integration plasmid expressing histone H3 (HHT1) with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18143<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18143\">PHM829<\/a><\/td>\n<td>Integration plasmid of histone H3 (HHT1) with a FLAG-tag to Ty1 loci.  Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18144<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18144\">PHM820<\/a><\/td>\n<td>Integration plasmid expressing Venus with a FLAG-tag to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18145<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18145\">PHM833<\/a><\/td>\n<td>Integration plasmid expressing indolepyruvate decarboxylase 1 (PDC1) to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18146<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18146\">PHM821<\/a><\/td>\n<td>Integration plasmid of histone H3 (HHT1) and Venus with a FLAG-tag to Ty1 loci.  Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<tr>\n<td>RDB18147<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18147\">PHM802<\/a><\/td>\n<td>Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR\/Transposon gene integration (CRITGI) gene expression technology clone.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h4><i>Saccharomyces cerevisiae<\/i>-E. coli shuttle vector, replicating with a low copy number in E. coli<\/h4>\n<p style=\"text-decoration: underline;\">Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals.<\/p>\n<p>Iida, K., Teng, J., Tada, T., Saka, A., Tamai, M., Izumi-Nakaseko, H., Adachi-Akahane, S., Iida, H.<br \/>\nJ. Biol. Chem. 282 (35): 25659-25667 (2007). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/17569661\">PubMed PMID 17569661<\/a>.<\/p>\n<table width=\"800\" border=\"1\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name<\/th>\n<th>Running title<\/th>\n<\/tr>\n<tr>\n<td>RDB15296<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15296\">pBC111<\/a><\/td>\n<td>S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h4>Empty expression vector designed to produce GFP-tagged protein<\/h4>\n<p style=\"text-decoration: underline;\">Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1<\/p>\n<p>Sasaki, T., Takagi, H.<br \/>\nGenes Cells, 18 (6): 459-475 (2013). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23517290\">PubMed PMID 23517290<\/a>.<\/p>\n<table width=\"800\" border=\"1\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name<\/th>\n<th>Running title<\/th>\n<\/tr>\n<tr>\n<td>RDB08380<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08380\">pRS416-PADH1-ccdB-yEGFP-TTEF1<\/a><\/td>\n<td>Empty expression vector backbone of S. cerevisiae.Gateway(R) destination vector.Contains ccdB and use DB3.1 or an equivalent host bacteria.<\/td>\n<\/tr>\n<tr>\n<td>RDB08386<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08386\">pRS416-PADH1-yEGFP-TTEF1<\/td>\n<td>Empty expression vector backbone of S. cerevisiae<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h4>Expression vector of S. cerevisiae Singlecopy plasmid<\/h4>\n<p style=\"text-decoration: underline;\">Overexpression of PRA2, a Rab\/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants.<\/p>\n<p>Matsuda, N., Ueda, T., Sasaki, Y., Nakano, A.<br \/>\nCell. Struct. Funct. 25 (1): 11-20 (2000). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/10791890\">PubMed PMID 10791890<\/a>.<\/p>\n<table width=\"800\" border=\"1\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name<\/th>\n<th>Running title<\/th>\n<\/tr>\n<tr>\n<td>RDB08655<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08655\">pSKY5<\/a><\/td>\n<td>Expression vector of S. cerevisiae. Single copy plasmid harboring the TDH3 promoter, EGFP, CMK1 terminator and the URA3 marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB08752<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08752\">pYO773<\/a><\/td>\n<td>Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the URA3 marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB08753<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08753\">pNMV3<\/td>\n<td>Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the HIS3 marker.<\/td>\n<\/tr>\n<tr>\n<td>RDB08754<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08754\">pNMV4<\/td>\n<td>Expression vector of S. cerevisiae. Singlecopy plasmid harboring the GAL1 promoter and the TRP1 marker.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<h4>Expression vector designed to produce C-terminal-Myc-tagged fusion proteins<\/h4>\n<p style=\"text-decoration: underline;\">Shuttle-vector system for <i>Saccharomyces cerevisiae<\/i> designed to produce C-terminal-Myc-tagged fusion proteins<\/p>\n<p>Iha, H., Tsurugi, K.<br \/>\nBiotechniques, 25, 936-938 (1998). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/9863043\">PubMed PMID 9863043<\/a>.<\/p>\n<table width=\"800\" border=\"1\">\n<tbody>\n<tr>\n<th>Catalog no.<\/th>\n<th>Name<\/th>\n<th>Tag<\/th>\n<th>Ori<\/th>\n<th>Marker<\/th>\n<th>Promoter<\/th>\n<th>Size (kb)<\/th>\n<th>Usable sites<\/th>\n<\/tr>\n<tr>\n<td>RDB01947<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01947\">pAMH10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td>HIS3<\/td>\n<td>ADH1<\/td>\n<td>4.5<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01948<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01948\">pAMH20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>HIS3<\/td>\n<td>ADH1<\/td>\n<td>5.3<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01949<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01949\">pAML10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td>LEU2<\/td>\n<td>ADH1<\/td>\n<td>5.1<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01950<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01950\">pAML20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>LEU2<\/td>\n<td>ADH1<\/td>\n<td>5.9<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01951<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01951\">pAMT10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td>TRP1<\/td>\n<td>ADH1<\/td>\n<td>4.3<\/td>\n<td>5<\/td>\n<\/tr>\n<tr>\n<td>RDB01952<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01952\">pAMT20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>TRP1<\/td>\n<td>ADH1<\/td>\n<td>5.1<\/td>\n<td>5<\/td>\n<\/tr>\n<tr>\n<td>RDB01953<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01953\">pAMU10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td>URA3<\/td>\n<td>ADH1<\/td>\n<td>4.6<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01954<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01954\">pAMU20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>URA3<\/td>\n<td>ADH1<\/td>\n<td>5.4<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01955<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01955\">pGMH10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td> HIS3<\/td>\n<td>GAL1-10<\/td>\n<td>4.8<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01956<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01956\">pGMH20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>HIS3<\/td>\n<td>GAL1-10<\/td>\n<td>5.6<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01957<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01957\">pGML10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td>LEU2<\/td>\n<td>GAL1-10<\/td>\n<td>5.4<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01958<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01958\">pGML20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td> LEU2<\/td>\n<td>GAL1-10<\/td>\n<td>6.2<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01959<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01959\">pGMT10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td>TRP1<\/td>\n<td>GAL1-10<\/td>\n<td>4.6<\/td>\n<td>5<\/td>\n<\/tr>\n<tr>\n<td>RDB01960<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01960\">pGMT20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>TRP1<\/td>\n<td>GAL1-10<\/td>\n<td>5.4<\/td>\n<td>5<\/td>\n<\/tr>\n<tr>\n<td>RDB01961<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01961\">pGMU10<\/a><\/td>\n<td>Myc<\/td>\n<td>centromere-type<\/td>\n<td> URA3<\/td>\n<td>GAL1-10<\/td>\n<td>4.9<\/td>\n<td>6<\/td>\n<\/tr>\n<tr>\n<td>RDB01962<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01962\">pGMU20<\/a><\/td>\n<td>Myc<\/td>\n<td>2micron<\/td>\n<td>URA3<\/td>\n<td>GAL1-10<\/td>\n<td>5.7<\/td>\n<td>6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Myc-tag (EQKLISEEDL) can be recognized by mouse monoclonal anitbody 9E10.<\/p>\n<hr \/>\n<p><Img Src=\"https:\/\/dna.brc.riken.jp\/en\/wp-content\/uploads\/images\/nbrp_s.png\" Width=\"64\" Border=\"0\" Align=\"AbsMiddle\"><a href=\"https:\/\/yeast.nig.ac.jp\/yeast\/\">National BioResource Project &#8211; Yeast <\/a><br \/>\nStrains and plasmids of budding yeast (<I>Saccharomyces cerevisiae<\/I>) is available.<\/p>\n<p><a href=\"#Head\"><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/goup.gif\" border=\"0\" \/> go to Head<\/a> <!-- - - - - - - - - - - End of Lower side - - - - - - - - - - - - --><\/p>\n<hr \/>\n<p style=\"text-align: left;\">2025.05.03 (T.M. GRP0047e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Vectors with fluorescent protein marker Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y. Front. Cell Dev. Biol. 8: 571388 (2020). PubMed PMID 33330450. (including unpublished bioresource) Catalog no. Name Running title RDB19364 pYU68 pRS313-ADH1p-MCS-GFP(S65T)-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. HIS3 marker. GFP(S65T)-tag at C-terminus. RDB19365 pYU69 pRS314-ADH1p-MCS-GFP(S65T)-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. TRP1 marker. GFP(S65T)-tag at C-terminus. RDB19366 pYU70 pRS315-ADH1p-MCS-GFP(S65T)-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. LEU2 marker. GFP(S65T)-tag at C-terminus. RDB19367 pYU71 pRS316-ADH1p-MCS-GFP(S65T)-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. GFP(S65T)-tag at C-terminus. RDB19368 pYU72 pRS313-ADH1p-MCS-mCherry-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. HIS3 marker. mCherry-tag at C-terminus. RDB19369 pYU73 pRS314-ADH1p-MCS-mCherry-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. TRP1 marker. mCherry-tag at C-terminus. RDB19370 pYU74 pRS315-ADH1p-MCS-mCherry-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. LEU2 marker. mCherry-tag at C-terminus. RDB19371 pYU75 pRS316-ADH1p-MCS-mCherry-CYC1ter Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at C-terminus. RDB19372 pYU76 pRS313-GPDp-MCS-GFP(S65T)-CYC1ter Yeast expression vector with the GPD1 promoter [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8681,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8709","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8709","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8709"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8709\/revisions"}],"predecessor-version":[{"id":8713,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8709\/revisions\/8713"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8681"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8709"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}