{"id":8698,"date":"2025-05-03T16:31:50","date_gmt":"2025-05-03T07:31:50","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8698"},"modified":"2025-05-03T16:32:31","modified_gmt":"2025-05-03T07:32:31","slug":"hostbacten","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/cloningen\/hostbacten","title":{"rendered":"Host strain &amp; Bacteria expression"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><br \/>\n<a name=\"grp0028e\"><\/a><\/p>\n<h3>E. coli strain for producing recombinant proteins incorporating synthetic amino acids<\/h3>\n<ul>\n<li>Dr. Kensaku Sakamoto and his colleagues at RIKEN Center for Biosystems Dynamics Research (RIKEN BDR) developed host E. coli strains that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.<\/li>\n<li>The RFzero-iy strains are designated to incorporate 3-iodotyrosine into produced proteins. For the incorporation, the strain is transformed with the expression plasmid in which a codon for the target tyrosine is replaced with UAG codon and cultured in 3-iodotyrosine containing medium (Mukai, T. et al., 2011). Two strains based on the BW25113 and BL-21(DE3) are available.<\/li>\n<li>The B-95.deltaA strains can be assigned their UAG codon to synthetic amino acids by the introduction of plasmids carrying specific pair of UAG-reading tRNA and an aminoacyl-tRNA synthetase (aaRS) variant*. Increased productivity of sulfonated hirudin which is expected to improve the inhibition of blood coagulation was reported (Mukai, T. et al., 2015). The original and the growth improved derivative strains are available.<\/li>\n<li>Web site<br \/>\n<a href=\"http:\/\/www.clst.riken.jp\/en\/science\/tech\/synthetic_amino_acid\/\">Incorporation of synthetic amino acids into proteins at specific sites<\/a><\/li>\n<li><span style=\"text-decoration: underline;\">Genetic-code evolution for protein synthesis with non-natural amino acids.<\/span><br \/>\nMukai T, Yanagisawa T, Ohtake K, Wakamori M, Adachi J, Hino N, Sato A, Kobayashi T, Hayashi A, Shirouzu M, Umehara T, Yokoyama S, Sakamoto K.<br \/>\nBiochem. Biophys. Res. Commun. 411 (4): 757-761, 2011. [PMID 21782790]<br \/>\n<span style=\"text-decoration: underline;\">Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon.<\/span><br \/>\nMukai T, Hoshi H, Ohtake K, Takahashi M, Yamaguchi A, Hayashi A, Yokoyama S, Sakamoto K.<br \/>\nSci. Rep. 5: 9699, 2015. [PMID 25982672]<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td>RDB13711<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13711\">B95. delta A<\/a><\/td>\n<td>Escherichia coli BL-21(DE3)-based host strain with no specific assignment of the UAG codon.<\/td>\n<\/tr>\n<tr>\n<td>RDB13712<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13712\">B95. delta A delta fabR<\/a><\/td>\n<td>Escherichia coli BL21(DE3)-based host strain with no specific assignment of the UAG codon.<\/td>\n<\/tr>\n<tr>\n<td>RDB14427<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14427\">BW25113-based RFzero-iy<\/a><\/td>\n<td>Escherichia coli host strain to produce non-natural amino acids containing proteins.<\/td>\n<\/tr>\n<tr>\n<td>RDB14428<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14428\">BL21(DE3)-based RFzero-iy<\/a><\/td>\n<td>Escherichia coli host strain to produce non-natural amino acids containing proteins.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><span style=\"text-decoration: underline;\">Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates.<\/span><br \/>\nYamaguchi A, Matsuda T, Ohtake K, Yanagisawa T, Yokoyama S, Fujiwara Y, Watanabe T, Hohsaka T, Sakamoto K.<br \/>\nBioconjug. Chem. 27 (1): 198-206, 2015. [PMID 26625213]<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td>RDB20115<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB20115\">pCDF-Az<\/a><\/td>\n<td>Expression vector of the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA.<\/td>\n<\/tr>\n<tr>\n<td>RDB20116<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB20116\">pCDF-Mm2<\/a><\/td>\n<td>Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr \/>\n<h3>E. coli auxotroph host strain used for selective isotope labeling<\/h3>\n<ul>\n<li>All ML strains werre engineered by Dr. Gennis research group at University of Illinois at Urbana-Champaign, U.S.A., and YM, MS, EH, and RF strains were engineered by Dr. Iwasaki research group at Nippon Medical School, Japan. This work was supported in part by the Internaitonal Collaborations in Chemistry Grant from JSPS and NSF, the Nagase Science and Technology Foundation Research Grant, and JSPS grants-in-aid.<br \/>\n<table>\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Strain name<\/th>\n<th>Genotype<\/th>\n<th>Amino acid requirement and\/or labeling<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td>RDB15927<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15927\">ML2<\/a><\/td>\n<td>cyo::kan ilvE;<\/td>\n<td>Ile, Leu.<\/td>\n<\/tr>\n<tr>\n<td>RDB15928<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15928\">ML3<\/a><\/td>\n<td>cyo::kan hisG<\/td>\n<td>His.<\/td>\n<\/tr>\n<tr>\n<td>RDB15929<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15929\">ML6<\/a><\/td>\n<td>cyo::kan ilvE avtA<\/td>\n<td>Ile, Leu, Val.<\/td>\n<\/tr>\n<tr>\n<td>RDB15930<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15930\">ML8<\/a><\/td>\n<td>cyo::kan argH<\/td>\n<td>Arg.<\/td>\n<\/tr>\n<tr>\n<td>RDB15931<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15931\">ML12<\/a><\/td>\n<td>cyo::kan ilvE avtA aspC<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val.<\/td>\n<\/tr>\n<tr>\n<td>RDB15932<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15932\">ML14<\/a><\/td>\n<td>tyrA<\/td>\n<td>(Tyr).<\/td>\n<\/tr>\n<tr>\n<td>RDB15933<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15933\">ML17<\/a><\/td>\n<td>glnA<\/td>\n<td>Gln.<\/td>\n<\/tr>\n<tr>\n<td>RDB15934<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15934\">ML21<\/a><\/td>\n<td>tyrA hisG<\/td>\n<td>(Tyr), His.<\/td>\n<\/tr>\n<tr>\n<td>RDB15935<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15935\">ML24<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG asnA<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His.<\/td>\n<\/tr>\n<tr>\n<td>RDB15936<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15936\">ML25<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG asnA asnB<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Asn.<\/td>\n<\/tr>\n<tr>\n<td>RDB15937<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15937\">ML26<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG argH<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Arg.<\/td>\n<\/tr>\n<tr>\n<td>RDB15938<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15938\">ML31<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG argH metA<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Arg, Met.<\/td>\n<\/tr>\n<tr>\n<td>RDB15939<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15939\">ML36<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG metA<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Met.<\/td>\n<\/tr>\n<tr>\n<td>RDB15940<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15940\">ML40<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG argH metA lysA<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Arg, Met, Lys.<\/td>\n<\/tr>\n<tr>\n<td>RDB15941<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15941\">ML42<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG argH metA lysA thrC asnB<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Arg, Met, Lys, Thr.<\/td>\n<\/tr>\n<tr>\n<td>RDB15942<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15942\">ML43<\/a><\/td>\n<td>cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB<\/td>\n<td>(Ala), Ile, Leu, Tyr, Val, His, Arg, Met, Lys, Thr, Asn.<\/td>\n<\/tr>\n<tr>\n<td>RDB15943<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15943\">YM138<\/a><\/td>\n<td>cysE<\/td>\n<td>Cys.<\/td>\n<\/tr>\n<tr>\n<td>RDB15944<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15944\">MS1<\/a><\/td>\n<td>cysE hisG<\/td>\n<td>Cys, His.<\/td>\n<\/tr>\n<tr>\n<td>RDB15952<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15952\">RF11<\/a><\/td>\n<td>metA<\/td>\n<td>Met.<\/td>\n<\/tr>\n<tr>\n<td>RDB15945<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15945\">RF1<\/a><\/td>\n<td>glyA<\/td>\n<td>Gly.<\/td>\n<\/tr>\n<tr>\n<td>RDB15946<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15946\">RF2<\/a><\/td>\n<td>thrC<\/td>\n<td>Thr.<\/td>\n<\/tr>\n<tr>\n<td>RDB15947<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15947\">RF4<\/a><\/td>\n<td>aspC tyrB<\/td>\n<td>Asp, Tyr, (Phe).<\/td>\n<\/tr>\n<tr>\n<td>RDB15948<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15948\">RF5<\/a><\/td>\n<td>aspC tyrB hisG<\/td>\n<td>Asp, Tyr, (Phe), His.<\/td>\n<\/tr>\n<tr>\n<td>RDB15949<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15949\">RF6<\/a><\/td>\n<td>proC<\/td>\n<td>Pro.<\/td>\n<\/tr>\n<tr>\n<td>RDB15950<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15950\">RF8<\/a><\/td>\n<td>asnA asnB<\/td>\n<td>Asn.<\/td>\n<\/tr>\n<tr>\n<td>RDB15951<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15951\">RF10<\/a><\/td>\n<td>lysA<\/td>\n<td>Lys.<\/td>\n<\/tr>\n<tr>\n<td>RDB15953<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15953\">RF12<\/a><\/td>\n<td>trpA trpB<\/td>\n<td>Trp.<\/td>\n<\/tr>\n<tr>\n<td>RDB15954<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15954\">RF13<\/a><\/td>\n<td>aspC tyrB trpA trpB<\/td>\n<td>Asp, Tyr, Trp, (Phe).<\/td>\n<\/tr>\n<tr>\n<td>RDB15955<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15955\">RF15<\/a><\/td>\n<td>aspC tyrB trpA trpB glyA serB<\/td>\n<td>Asp, Tyr, Trp, (Phe), Gly, Ser.<\/td>\n<\/tr>\n<tr>\n<td>RDB15956<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15956\">RF16<\/a><\/td>\n<td>aspC tyrB trpA trpB glyA serB cysE<\/td>\n<td>Asp, Tyr, Trp, (Phe), Gly, Ser, Cys, Ala.<\/td>\n<\/tr>\n<tr>\n<td>RDB15957<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15957\">RF17<\/a><\/td>\n<td>aspC tyrB ilvE<\/td>\n<td>Asp, Tyr, Phe, Ile, Leu.<\/td>\n<\/tr>\n<tr>\n<td>RDB15958<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15958\">RF18<\/a><\/td>\n<td>aspC tyrB ilvE avtA<\/td>\n<td>Asp, Tyr, Phe, Ile, Leu, Val.<\/td>\n<\/tr>\n<tr>\n<td>RDB15959<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15959\">RF21<\/a><\/td>\n<td>aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC)<\/td>\n<td>Asp, Tyr, Phe, Ile, Leu, Val.<\/td>\n<\/tr>\n<tr>\n<td>RDB15960<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15960\">EH1<\/a><\/td>\n<td>thrC ilvA<\/td>\n<td>Thr, Ile.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Please refer web site of <a href=\"https:\/\/fesworld.jp\/EcoliStrains.html\">Escherichia coli auxotrophic expression strains (by the depositor)<\/a>, Lin et al., Methods 55 (4): 370-378 (2011), Iwasaki et al., J. Am. Chem. Soc. 134 (48): 19731-19738 (2012), and\/or Lin et al., Methods Enzymol. 565: 45-66 (2015). <\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<h3>Escherichia coli host and vectors<\/h3>\n<ul style=\"line-height: 2em;\">\n<li>Expression, purification and characterization of a photoprotein, clytin, from Clytia gregarium.<br \/>\nInouye, S., Sahara, Y.<br \/>\nProtein Expr. Purif. 53 (2): 384-389 (2007). PMID 17275329.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2023,5,2)<\/script>RDB19856<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19856\">piP-H-XEL<\/a><\/td>\n<td>Bacterial expression vector. Secretion vector with OmpA signal peptide sequence and His-Tag under the control of Ipp promoter in E. coli.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli.<br \/>\nMiyazaki, K.<br \/>\nPLoS One 13 (4): e0194090 (2018). PubMed PMID 29641575.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td>RDB17946<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17946\">pSAL7A35VS<\/a><\/td>\n<td>Bacterial expression vector for the salicylate-inducible high expression system in E. coli.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli.<br \/>\nIhara, K., Sato, K., Hori, H., Makino, Y., Shigenobu, S., Ando, T., Isogai, E., Yoneyama, H.<br \/>\nJ. Biosci. Bioeng. 123 (4): 444-450 (2017). PubMed PMID 28057466.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td>RDB15921<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15921\">MG1655 delta lrp<\/a><\/td>\n<td>Escherichia coli host strain. MG1655 with a deletion in lrp gene.<\/td>\n<\/tr>\n<tr>\n<td>RDB15922<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15922\">MG1655 delta lacZ<\/a><\/td>\n<td>Escherichia coli host strain. MG1655 with a deletion in lacZ.<\/td>\n<\/tr>\n<tr>\n<td>RDB15923<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15923\">MG1655 delta lacZ delta lrp<\/a><\/td>\n<td>Escherichia coli host strain. MG1655 with deletion in lrp and lacZ genes<\/td>\n<\/tr>\n<td>RDB15924<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15924\">pQE-Lrp<\/a><\/td>\n<td>Expression vector of Escherichia coli K-12 MG1655 leucine-responsive regulatory protein (Lrp).<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Improvement of (R)-1,3-butanediol production by engineered Escherichia coli.<br \/>\nKataoka, N., Vangnai, A.S., Tajima, T., Nakashimada, Y., Kato, J.<br \/>\nJ. Biosci. Bioeng. 115(5): 475-480 (2013). PubMed PMID 23290993.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15466<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15466\">pNK<\/a><\/td>\n<td>E. coli expression vector, PA1lacO-1 promoter, rrnBT1T2 terminator, p15A and pAM alpha1 origin.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates.<br \/>\nFukunaga, R., Hunter, T.<br \/>\nEMBO J., 16, 1921-1933 (1997). PubMed PMID 9155018.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB01911<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01911\">Lambda GEX5<\/a><\/td>\n<td>Lambda phage cloning vector producing GST fusion protein.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1.<br \/>\nMurata, T., Shinozuka, Y., Obata, Y., Yokoyama, K.K.<br \/>\nAnal. Biochem., 376, 115-121 (2008). PubMed PMID 18307971.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB06211<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06211\">pT7Tet1<\/a><\/td>\n<td>Expression vector, bacterial.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<ul>\n<li>Other vectors<br \/>\n<table border=\"1\">\n<tbody>\n<tr>\n<th rowspan=\"2\">Catalog num.<\/th>\n<th rowspan=\"2\">Resource name<\/th>\n<th rowspan=\"2\">ori<\/th>\n<th colspan=\"3\">Registance (E. coli)<\/th>\n<th rowspan=\"2\">Cloning<\/th>\n<th colspan=\"2\">Promoter<\/th>\n<th colspan=\"3\">Tag<\/th>\n<\/tr>\n<tr>\n<th>Amp<\/th>\n<th>Tet<\/th>\n<th>Cm<\/th>\n<th>lac<\/th>\n<th>T7<\/th>\n<th>FLAG<\/th>\n<th>His<\/th>\n<th>GST<\/th>\n<\/tr>\n<tr>\n<td>RDB06084<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06084\">pETEG5<\/a><\/td>\n<td>pBR322<\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><\/td>\n<td>Restriction Enzyme<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>RDB06211<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06211\">pT7Tet1<\/a><\/td>\n<td>p15A<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td>Restriction Enzyme<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>RDB06212<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB06212\">pT7Tet6<\/a><\/td>\n<td>p15A<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td>Restriction Enzyme<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>RDB07041<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07041\">pETHFFc<\/a><\/td>\n<td>pBR322<\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><\/td>\n<td>Gateway&reg;<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>RDB07050<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07050\">pETF<\/a><\/td>\n<td>pBR322<\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><\/td>\n<td>Restriction Enzyme<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>RDB07051<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07051\">pT7FCam<\/a><\/td>\n<td>p15A<\/td>\n<td><\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td>Restriction Enzyme<\/td>\n<td><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><img decoding=\"async\" alt=\"\" src=\"https:\/\/dnaconda.riken.jp\/images\/sumi02.gif\" \/><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr width=\"50%\" \/>\n<ul>\n<li><a href=\"https:\/\/shigen.nig.ac.jp\/ecoli\/strain\/\">DNA resources as well as E. coli strains [link]<\/a> are available at &#8220;E.coli Strains, National BioResource Project&#8221;, National Institute of Genetics.<\/li>\n<\/ul>\n<hr \/>\n<h3>Bifidobacterium longum bacterial host and vectors<\/h3>\n<ul style=\"line-height: 2em;\">\n<li>Construction of Escherichia coli-Bifidobacterium longum shuttle vector transforming B. longum 105-A and 108-A.<br \/>\nMatsumura, H., Takeuchi, A., Kano, Y.<br \/>\nBiosci. Biotechnol. Biochem. 61 (7): 1211-1212 (1997). PubMed PMID 9255988.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>JCM 31944<\/td>\n<td><a href=\"https:\/\/www.jcm.riken.jp\/cgi-bin\/jcm\/jcm_number?JCM=31944\">105-A<\/a><\/td>\n<td>Host strain of Bifidobacterium longum 105-A available from JCM.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the \u03b1-galactosidase gene for raffinose assimilation.<br \/>\nHirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A.<br \/>\nAppl. Environ. Microbiol. 78 (14): 4984-4994 (2012). PubMed PMID 22582061. <\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15972<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15972\">pTBR101-CM<\/a><\/td>\n<td>Bifidobacterium longum shuttle vector<\/td>\n<\/tr>\n<tr>\n<td>RDB15973<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15973\">pBS423 delta repA<\/a><\/td>\n<td>Bifidobacterium longum shuttle vector<\/td>\n<\/tr>\n<td>RDB15974<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15974\">pBS423<\/a><\/td>\n<td>Bifidobacterium longum shuttle vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr \/>\n<h3>Bacillus stearothermophilus bacterial host and vectors<\/h3>\n<ul style=\"line-height: 2em;\">\n<li>A newly isolated Bacillus stearothermophilus K1041 and its transformation by electroporation.<br \/>\nNarumi, I., Sawakami, K., Nakamoto, S., Nakayama, N., Yanagisawa, T., Takahashi, N., Kihara, H.<br \/>\nBiotechnol. Tech. 6, 83-86 (1992). <\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB00139<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB00139\">K1041<\/a><\/td>\n<td>Host strain of Bacillus stearothermophilus for pSTE33.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Nakayama, N., A new shuttle vector for Bacillus stearothermophilus and Escherichia coli.<br \/>\nNakayama, N., Narumi, I., Nakamoto, S., Kihara, H.<br \/>\nBiotechnol. letters, 14, 649-652 (1992). <\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB00919<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB00919\">pSTE12<\/a><\/td>\n<td>Bacillus stearothermophilus shuttle vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Construction of a new shuttle vector pSTE33 and its stabilities in Bacillus stearothermophilus, Baciluss subtilis, and Escherichia coli.<br \/>\nNarumi, I., Nakayama, N., Makamoto, S., Kimura, T., Yanagisawa, T., Kihara, H.<br \/>\nBiotechnol. letters, 15, 815-820 (1993). <\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB00920<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB00920\">pSTE33<\/a><\/td>\n<td>Bacillus stearothermophilus shuttle vector<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li><a href=\"https:\/\/shigen.nig.ac.jp\/bsub\/\">DNA resources as well as Bacillus subtilis strains [link]<\/a> are available at &#8220;Bacillus subtilis, National BioResource Project&#8221;, National Institute of Genetics.<\/li>\n<\/ul>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.03 T.M. GRP0028e)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>E. coli strain for producing recombinant proteins incorporating synthetic amino acids Dr. Kensaku Sakamoto and his colleagues at RIKEN Center for Biosystems Dynamics Research (RIKEN BDR) developed host E. coli strains that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon. The RFzero-iy strains are designated to incorporate 3-iodotyrosine into produced proteins. For the incorporation, the strain is transformed with the expression plasmid in which a codon for the target tyrosine is replaced with UAG codon and cultured in 3-iodotyrosine containing medium (Mukai, T. et al., 2011). Two strains based on the BW25113 and BL-21(DE3) are available. The B-95.deltaA strains can be assigned their UAG codon to synthetic amino acids by the introduction of plasmids carrying specific pair of UAG-reading tRNA and an aminoacyl-tRNA synthetase (aaRS) variant*. Increased productivity of sulfonated hirudin which is expected to improve the inhibition of blood coagulation was reported (Mukai, T. et al., 2015). The original and the growth improved derivative strains are available. Web site Incorporation of synthetic amino acids into proteins at specific sites Genetic-code evolution for protein synthesis with non-natural amino acids. Mukai T, Yanagisawa T, Ohtake K, Wakamori M, Adachi J, Hino N, Sato A, Kobayashi [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8681,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8698","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8698","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8698"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8698\/revisions"}],"predecessor-version":[{"id":8702,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8698\/revisions\/8702"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8681"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8698"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}