{"id":8692,"date":"2025-05-03T16:29:12","date_gmt":"2025-05-03T07:29:12","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8692"},"modified":"2025-05-03T16:30:59","modified_gmt":"2025-05-03T07:30:59","slug":"expressvecen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/cloningen\/expressvecen","title":{"rendered":"Expression vector"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<p style=\"text-align:center;\">| <a href=\"#mammalianvector\">Mammalian<\/a> | <a href=\"#shRNA\">shRNA expression<\/a> | <a href=\"#invitro_txn\">in vitro transcrioption<\/a> | <a href=\"#supernova\">Supernova series<\/a> | <a href=\"#gene_amp\">Gene amplification<\/a> | <a href=\"#insect\">Insect cell<\/a> | <a href=\"#plant\">Plant<\/a> |<br \/>\nSee also &#8220;<a href=\"\/en\/viralvecen\/\">Viral &#038; Transposon Vector Plasmids (backbone)<\/a>&#8220;.<\/p>\n<hr width=\"50%\" \/>\n<hr \/>\n<p><a name=\"mammalianvector\"><\/a><\/p>\n<h4>Mammalian expression<\/h4>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.&nbsp;Clone name<br \/>Description<\/th>\n<th>Epitope tag<\/th>\n<th>Antibiotic\/visual marker<\/th>\n<th>Promoter<\/th>\n<th>Cloning<\/th>\n<th>Antibiotics (bacteria)<\/th>\n<\/tr>\n<tr>\n<td>RDB07939&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07939\">pEF-BOS<\/a><br \/>Mammalian expression vector with human EF-1a.<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07940&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07940\">pEF-BOS-EX<\/a><br \/>Mammalian expression vector with human EF-1a.<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<td><script>newImage(2021,8,30)<\/script>RDB18970&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18970\">pNEF-BOS-EX<\/a><br \/>Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene.<\/td>\n<td>n.a.<\/td>\n<td>G418<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,8,30)<\/script>RDB18971&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18971\">pPEF-BOS-EX<\/a><br \/>Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene.<\/td>\n<td>n.a.<\/td>\n<td>puromycin<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,8,30)<\/script>RDB18972&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18972\">pEF-Flag-EX<\/a><br \/>Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein.<\/td>\n<td>FLAG\n<td>n.a.<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,8,30)<\/script>RDB18973&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18973\">pEF321-Flag-Ex<\/a><br \/>Mammalian expression vector based on human EF-1 alpha promoter.<\/td>\n<td>FLAG\n<td>n.a.<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,8,30)<\/script>RDB18974&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB18974\">pEF-Fc<\/a><br \/>Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region.<\/td>\n<td>n.a.\n<td>n.a.<\/td>\n<td>EF-1 alpha<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07939\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB07\/RDB07939_map.png\" alt=\"\" width=\"300\" \/><\/a><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07940\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB07\/RDB07940_map.png\" alt=\"\" width=\"300\" \/><\/a><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18970\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB18\/RDB18970_map.png\" alt=\"\" width=\"300\" \/><\/a><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18971\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB18\/RDB18971_map.png\" alt=\"\" width=\"300\" \/><\/a><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18972\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB18\/RDB18972_map.png\" alt=\"\" width=\"300\" \/><\/a><br \/>\n&nbsp;<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.&nbsp;Clone name<br \/>Description<\/th>\n<th>Epitope tag<\/th>\n<th>Antibiotic\/visual marker<\/th>\n<th>Promoter<\/th>\n<th>Cloning<\/th>\n<th>Antibiotics (bacteria)<\/th>\n<\/tr>\n<tr>\n<td>RDB14213&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14213\">pcDNA3.1-myc-GFP<\/a><br \/>Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.<\/td>\n<td>myc-GFP<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB14401&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14401\">pCIneoEGFPC2<\/a><br \/>Plasmid vector for constructing GFP-tagged expression vector.<\/td>\n<td>EGFP<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB05956&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB05956\">pCMV_S-FLAG<\/a><br \/>Expressing FLAG-tagged fusion protein, CMV promoter.<\/td>\n<td>FLAG<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB06083&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB06083\">pRSV_S-FLAG<\/a><br \/>Expressing FLAG-tagged fusion protein, RSV promoter.<\/td>\n<td>FLAG<\/td>\n<td>Neo<\/td>\n<td>RSV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07396&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07396\">pCMV_S-FLAGc<\/a><br \/>Destination vector to generate eukaryotic expression vector, with FLAG-tag.<\/td>\n<td>FLAG<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Gateway(R)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07397&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07397\">pRSV_s-FLAGc<\/a><br \/>Destination vector to generate eukaryotic expression vector, with FLAG-tag.<\/td>\n<td>FLAG<\/td>\n<td>Neo<\/td>\n<td>RSV<\/td>\n<td>Gateway(R)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<td>RDB14218&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14218\">pcDNA5-FLAG\/HA\/SBP<\/a><br \/>Expression vector of N-terminally FLAG\/HA\/SBP tagged proteins in mammalian cells.<\/td>\n<td>FLAG\/HA\/SBP<\/td>\n<td>Hygro<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB10461&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB10461\">pBRHCAG-cHA-pA<\/a><br \/>Expression vector with CAG promoter, HA-tag, hygromycin selection (hph).<\/td>\n<td>HA<\/td>\n<td>Hygro<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB02136&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02136\">S-HA-pRc\/CMV<\/a><br \/>Expressing HA-tagged fusion protein, CMV promoter.<\/td>\n<td>HA<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB02139&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02139\">D-HA-pRc\/CMV<\/a><br \/>Expressing a fusion protein tagged with duplicated HA, CMV promoter.<\/td>\n<td>2xHA<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07105&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07105\">pBRPyCAG-cHA-IN<\/a><br \/>Expression vector with CAG promoter, HA-tag, Neoycin selection.<\/td>\n<td>HA<\/td>\n<td>Neo<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB11391&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB11391\">pPyCAG-cHA-IN<\/a><br \/>Expression vector with CAG promoter, HA-tag, Neomycin selection.<\/td>\n<td>HA<\/td>\n<td>Neo<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB12114&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12114\">pPyCAG-cHA-IpacflexEGFP<\/a><br \/>Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker.<\/td>\n<td>HA<\/td>\n<td>Puro &amp; EGFP<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07101&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07101\">pBRPyCAG-cHA-IP<\/a><br \/>Expression vector with CAG promoter, HA-tag, Puromycin selection.<\/td>\n<td>HA<\/td>\n<td>Puro<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07100&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07100\">pPyCAG-cHA-IP<\/a><br \/>Expression vector with CAG promoter, HA-tag, Puromycin selection.<\/td>\n<td>HA<\/td>\n<td>Puro<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB10479&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB10479\">pPyPGK-cHA-IP<\/a><br \/>Expression vector with mouse PGK promoter, HA-tag, IRESpuro.<\/td>\n<td>HA<\/td>\n<td>Puro<\/td>\n<td>PGK<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB10460&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB10460\">pBR-PyCAG-cHA-IP SVOri<\/a><br \/>Expression vector with CAG promoter, HA-tag, Puromycin selection.<\/td>\n<td>HA<\/td>\n<td>Puro<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB10435&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB10435\">pBR-CAG-cHA-pA<\/a><br \/>Expression vector with CAG promoter, HA-tag.<\/td>\n<td>HA<\/td>\n<td>n.a.<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB02140&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02140\">S-Myc-pRc\/CMV<\/a><br \/>Expressing Myc-tagged fusion protein, CMV promoter.<\/td>\n<td>Myc<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB02141&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02141\">D-Myc-pRc\/CMV<\/a><br \/>Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter.<\/td>\n<td>2xMyc<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB02137&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02137\">S-T7-pRc\/CMV<\/a><br \/>Expressing T7-tagged fusion protein, CMV promoter.<\/td>\n<td>T7<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB02138&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB02138\">D-T7-pRc\/CMV<\/a><br \/>Expressing a fusion protein tagged with duplicated T7, CMV promoter.<\/td>\n<td>2xT7<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB12113&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12113\">pPyCAG-BstXI-ZeoP2AEGFP<\/a><br \/>Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker.<\/td>\n<td>n.a.<\/td>\n<td>Bleo(Zeo) &amp; EGFP<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme(BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09315&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09315\">pPyCAG-IRESzeocinpA (+SVori)<\/a><br \/>Expression vector with CAG promoter, IRES-zeo.<\/td>\n<td>n.a.<\/td>\n<td>Bleo(Zeo)<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme (BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09297&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09297\">pZPCAGGS<\/a><br \/>Expression vector with CAG promoter, Zeo marker.<\/td>\n<td>n.a.<\/td>\n<td>Bleo(Zeo)<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme (BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB12107&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12107\">pPyCAG-BstXI-IB<\/a><br \/>Mammalian expression vector. Blasticidin resistance.<\/td>\n<td>n.a.<\/td>\n<td>BSD<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme(BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB12112&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12112\">pPyCAG-BstXI-IHisDflexEGFP<\/a><br \/>Expression vector with CAG promoter, HA-tag, HisD selection. GFP marker.<\/td>\n<td>n.a.<\/td>\n<td>HisD &amp; EGFP<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme(BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB10322&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB10322\">pPyCAG-BstXI-IRSHisDpA<\/a><br \/>Expression vector of IRES-HisD with CAG promoter.<\/td>\n<td>n.a.<\/td>\n<td>HisD<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme (BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB12108&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12108\">pPyCAG-BstXI-HygP2AEGFP<\/a><br \/>Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker.<\/td>\n<td>n.a.<\/td>\n<td>Hygro &amp; EGFP<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme(BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09233&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09233\">pPyCAG-BstXI-IREShygropA<\/a><br \/>Expression vector of CAG promoter, IRES-hygromycin resistance.<\/td>\n<td>n.a.<\/td>\n<td>Hygro<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme(BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09694&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09694\">pCAGGSneo-delta-EcoRI<\/a><br \/>Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker.<\/td>\n<td>n.a.<\/td>\n<td>Neo<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB07099&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB07099\">pPyCAG-BstXI-IP<\/a><br \/>Mammalian expression vector. Blasticidin resistance.<\/td>\n<td>n.a.<\/td>\n<td>Puro<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme(BstXI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB01968&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB01968\">pBMSA<\/a><br \/>Expression vector, inducible MMTV-LTR promoter.<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>MMTV-LTR<\/td>\n<td>Restriction enzyme (XbaI)<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB08938&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08938\">pCAGGS<\/a><br \/>Mammalian expression vector with CAG promoter.<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>CAG<\/td>\n<td>Restriction enzyme<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB14136&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB14136\">pIDT-SMART(C-TSC)<\/a><br \/>Mammalian xpression vector with the improved high expression cassette C-TSC.<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>CMV-TSC<\/td>\n<td>Restriction enzyme<\/td>\n<td>Kan<\/td>\n<\/tr>\n<tr>\n<td>RDB19684&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19684\">pMWcmvT7-KpnI-pA<\/a><br \/>Infusion\/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial strictly low copy number plasmid (pMW219 backbone).<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>CMV<\/td>\n<td>Infusion\/Gibson assembly<\/td>\n<td>Kan<\/td>\n<\/tr>\n<tr>\n<td>RDB19683&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19683\">pBRcmvT7-KpnI-pA<\/a><br \/>Infusion\/Gibson assembly platform vectorfor dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial relatively low copy number plasmid (pBR322 backbone). Tetracyclin resistance gene has been truncated.<\/td>\n<td>n.a.<\/td>\n<td>n.a.<\/td>\n<td>CMV<\/td>\n<td>Infusion\/Gibson assembly<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB19682&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB19682\">pcmvT7-KpnI-polyA<\/a><br \/>Infusion\/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial high copy number plasmid (pCDNA3 backbone).<\/td>\n<td>n.a.<\/td>\n<td>Neo<\/td>\n<td>CMV<\/td>\n<td>Infusion\/Gibson assembly<\/td>\n<td>Amp<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul>\n<li><a href=\"https:\/\/brc.riken.jp\/dna\/GRP0049j\">pCMV_tag and pRSV_tag vectors (ja)<\/a>&nbsp;|&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/GRP0049e\">pCMV_tag and pRSV_tag vectors (en)<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB01379<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01379\">pH2Rneo<\/a><\/td>\n<td>Expression vector with simian virus 40 (SV40) early promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB01679<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01679\">pCALwL<\/a><\/td>\n<td>A cassette plasmid to insert cDNA into downstream CAG promoter<\/td>\n<\/tr>\n<tr>\n<td>RDB01862<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB01862\">pCALNL5<\/a><\/td>\n<td>A cassette to introduce cDNA into mammalian chromosome<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h4>Vector for marker knock-in (genome editing)<\/h4>\n<ul>\n<li>Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.<br \/>\nNatsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.<br \/>\nCell Rep. 15 (1): 210-218 (2016). PubMed PMID 27052166.<\/p>\n<table>\n<tbody>\n<tr>\n<th rowspan=\"2\">Selection marker<\/th>\n<th colspan=\"3\">Attached tag at the N-terminus<\/th>\n<\/tr>\n<tr>\n<th>mClover<\/th>\n<th>mCherry2<\/th>\n<th>S tag-3FLAG<\/th>\n<\/tr>\n<tr>\n<th>Hygro<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16814\">pMK376<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16815\">pMK377<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16813\">pMK375<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<th rowspan=\"2\">Selection marker<\/th>\n<th colspan=\"3\">Attached tag at the C-terminus<\/th>\n<\/tr>\n<tr>\n<th>mClover<\/th>\n<th>mCherry2<\/th>\n<th>S tag-3FLAG<\/th>\n<\/tr>\n<tr>\n<th>Neo<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13920\">pMK277<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13923\">pMK280<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13926\">pMK283<\/a><\/td>\n<\/tr>\n<tr>\n<th>Hygro<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13921\">pMK278<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13924\">pMK281<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13927\">pMK284<\/a><\/td>\n<\/tr>\n<tr>\n<th>BSD<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16816\">pMK388<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16817\">pMK389<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB16818\">pMK390<\/a><\/td>\n<\/tr>\n<tr>\n<th>Bsr<\/th>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13922\">pMK279<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13925\">pMK282<\/a><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13928\">pMK285<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use these clones.<\/p><\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"shRNA\"><\/a><\/p>\n<h4>shRNA expression<\/h4>\n<table>\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB04395<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB04395\">pENTR4-H1<\/a><\/td>\n<td>Expression vector of RNAi driven by human H1 promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB07916<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB07916\">pENTR4-H1tetOx1<\/a><\/td>\n<td>Expression vector of RNAi driven by human H1 promoter.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>See also Gateway&reg; destination vector in &#8220;<a href=\"\/en\/viralvecen\/lentien\/lenti_listen#shRNA\">Lentivirus Vector Plasmid<\/a>&#8220;. <\/p>\n<hr \/>\n<p><a name=\"invitro_txn\"><\/a><\/p>\n<h4>in vitro transcrioption<\/h4>\n<ul>\n<li>Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds.<br \/>\nWatanabe, T., Yamazaki, S., Yoneda, N., Shinohara, H., Tomioka, I., Higuchi, Y., Yagoto, M., Ema, M., Suemizu, H., Kawai, K., Sasaki, E.<br \/>\nGenes Cells 24 (7): 473-484 (2019). PubMed PMID 31099158.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB18330<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18330\">phBG<\/a><\/td>\n<td>Plasmid vector for in-vitro transcrioption, having two copies of 3&#8242; UTR DNA fragment of human hemoglobin subunit beta (HBB)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"supernova\"><\/a><\/p>\n<h4>Supernova series<\/h4>\n<p style=\"text-decoration: underline;\">\nSupernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.<\/p>\n<p>Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S,, Iwasato, T.<br \/>\n Sci. Rep. 6: 35747 (2016). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27775045\">PubMed PMID 27775045<\/a>.<\/p>\n<p style=\"text-decoration: underline;\">\nNMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates.<\/p>\n<p>Mizuno, H., Luo, W., Tarusawa, E., Saito, Y.M., Sato, T., Yoshimura, Y., Itohara, S., Iwasato, T.<br \/>\nNeuron 82 (2): 365-379 (2014). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24685175\">PubMed PMID 24685175<\/a>.<\/p>\n<table>\n<tbody>\n<tr>\n<td Align=\"Left\">Catalog #<\/td>\n<td Align=\"Left\">Name of clone<\/td>\n<td Align=\"Left\">Running title<\/td>\n<\/tr>\n<tr>\n<td>RDB15043<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15043\">pK031.TRE-Cre (Supernova)<\/a><\/td>\n<td>Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB15045<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15045\">pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)<\/a><\/td>\n<td>Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.<\/td>\n<\/tr>\n<tr>\n<td>RDB15046<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15046\">pK036.TRE-Flpe-WPRE (Supernova)<\/a><\/td>\n<td>Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB15047<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15047\">pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)<\/a><\/td>\n<td>Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter. <\/td>\n<\/tr>\n<tr>\n<td>RDB15050<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15050\">pK170.AAV-TRE-Cre-WPRE (Supernova)<\/a><\/td>\n<td>Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB15051<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15051\">pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)<\/a><\/td>\n<td>Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of loxP-stop-loxP-Cas9 under the CAG promoter. <\/td>\n<\/tr>\n<tr>\n<td>RDB15053<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15053\">pK073.TRE-Dre-WPRE (Supernova)<\/a><\/td>\n<td>Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase. <\/td>\n<\/tr>\n<tr>\n<td>RDB15055<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15055\">pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)<\/a><\/td>\n<td>Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter. <\/td>\n<\/tr>\n<tr>\n<td>RDB15056<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15056\">pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)<\/a><\/td>\n<td>Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed.<\/td>\n<\/tr>\n<tr>\n<td>RDB15057<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15057\">pK238.U6-gRNACreb1-CAG-loxP-stop-loxP-hspCas9 (Supernova)<\/a><\/td>\n<td>Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of both loxP-stop-loxP-Cas9 and gRNA (Creb1 as the target gene) under the CAG promoter. <\/td>\n<\/tr>\n<tr>\n<td>RDB15058<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15058\">pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova)  <\/a><\/td>\n<td>Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice.<\/td>\n<\/tr>\n<tr>\n<td>RDB15059<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15059\">pK178.CAG-loxP-stop-loxP-mir30 (GFP RNAi scramble control) (Supernova)  <\/a><\/td>\n<td>Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice.<\/td>\n<\/tr>\n<tr>\n<td>RDB15061<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15061\">pK229.AAV-EF1alpha-EGFP-WPRE (Supernova)<\/a><\/td>\n<td>Adeno-associated virus (AAV)-based Supernova series. This clone will be used as a control in AAV-based Supernova experiments.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p><a name=\"gene_amp\"><\/a><\/p>\n<h4>Plasmid for IR\/MAR gene amplification method and related plasmid.<\/h4>\n<table class=\"table_width\" id=\"header_table\">\n<tbody>\n<tr>\n<th>Catalog #<\/th>\n<th>Name of resource<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB18704<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18704.html\">pSFVdhfr<\/a><\/td>\n<td>A clone for the IR\/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells. <\/td>\n<\/tr>\n<tr>\n<td>RDB18705<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18705.html\">pdeltaBN.AR1<\/a><\/td>\n<td>A clone for the IR\/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells.<\/td>\n<\/tr>\n<tr>\n<td>RDB18706<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18706.html\">pdeltaBM.AR1.MCS<\/a><\/td>\n<td>A clone for the IR\/MAR gene amplification method. By cloning an expression unit of interested gene into the MCS, amplified gene expression can be obtained.<\/td>\n<\/tr>\n<tr>\n<td>RDB18707<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18707.html\">pdeltaBM.AR1.d2EGFP<\/a><\/td>\n<td>A clone for the IR\/MAR gene amplification method. This clone is used for monitoring the gene amplification by observation of GFP fluorescence.<\/td>\n<\/tr>\n<tr>\n<td>RDB18708<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18708.html\">pB-3-31<\/a><\/td>\n<td>Plasmid clone of 3,271 bp human genomic sequence, B-3-31, which is known to enhance the expression of co-transfected expression vectors.<\/td>\n<\/tr>\n<tr>\n<td>RDB18709<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18709.html\">pG5<\/a><\/td>\n<td>Plasmid clone of 972 bp human G5 sequence, which was isolated from beta-globin replication origin as a minimum sequence required for gene amplification.<\/td>\n<\/tr>\n<tr>\n<td>RDB18710<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18710.html\">pC12<\/a><\/td>\n<td>Plasmid clone of 798 bp human C12 sequence, which was isolated from MYC replication origin as a minimum sequence required for gene amplification.<\/td>\n<\/tr>\n<tr>\n<td>RDB18711<\/td>\n<td><a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB18\/RDB18711.html\">pD8<\/a><\/td>\n<td>Plasmid clone of 2,347 bp Chinese hamster D8 sequence, which was isolated from DHFR replication origin as a minimum sequence required for gene amplification.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul style=\"line-height: 2em;\">\n<li>Araki, Y., Efficient recombinant production in mammalian cells using a novel IR\/MAR gene amplification method. PLoS One, 7 (7): e41787 (2012). PMID 22844523. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=21536\">[link to RRC of NBRP]<\/a><\/li>\n<li>Fukuma, M., Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing. PLoS One 11 (4): e0153338 (2016). PMID 27078685. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=67371\">[link to RRC of NBRP]<\/a><\/li>\n<li>Harada, S., Episomal high copy number maintenance of hairpin-capped DNA bearing a replication initiation region in human cells. J. Biol. Chem. 284 (36): 24320-24327 (2009). PMID 19617622. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=67372\">[link to RRC of NBRP]<\/a><\/li>\n<li>Hashizume, T., Dissection of mammalian replicators by a novel plasmid stability assay. J. Cell Biochem. 101 (3): 552-565 (2007). PMID 17226771. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=67369\">[link to RRC of NBRP]<\/a><\/li>\n<li>Okada, N., Dissection of the beta-globin replication-initiation region reveals specific requirements for replicator elements during gene amplification. PLoS One 8 (10): e77350 (2013).  PMID 24124615. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=67370\">[link to RRC of NBRP]<\/a><\/li>\n<li>Shimizu, N., Plasmids with a mammalian replication origin and a matrix attachment region initiate the event similar to gene amplification. Cancer Res. 61 (19): 6987-6990 (2001). PMID 11585721. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=67374\">[link to RRC of NBRP]<\/a><\/li>\n<li>Shimizu, N., Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription. Cancer Res. 63 (17): 5281-5290 (2003). PMID 14500359. (original). <a href=\"https:\/\/rrc.nbrp.jp\/referenceDetailAction.do?referenceId=67373\">[link to RRC of NBRP]<\/a><\/li>\n<\/ul>\n<hr \/>\n<p><a name=\"insect\"><\/a><\/p>\n<h4 class=\"green\">Insect Cell Experiment<\/h4>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.&nbsp;Clone name<br \/>Description<\/th>\n<th>Epitope tag<\/th>\n<th>Antibiotic\/visual marker<\/th>\n<th>Cloning<\/th>\n<th>Promoter<\/th>\n<th>Bacterial resistance<\/th>\n<\/tr>\n<tr>\n<td>RDB08532&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08532\">pMT-PURO<\/a><br \/>Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.<\/td>\n<td>His-V5<\/td>\n<td>Puro<\/td>\n<td>Restriction enzyme<\/td>\n<td>MT<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB12152&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB12152\">pMT-PURO2<\/a><br \/>Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells<\/td>\n<td>His-V5<\/td>\n<td>Puro<\/td>\n<td>Restriction enzyme<\/td>\n<td>MT<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09002&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09002\">pMAK17<\/a><br \/>Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable.<\/td>\n<td>&#8211;<\/td>\n<td>Puro &amp; DsRed2<\/td>\n<td>&#8211;<\/td>\n<td>tight TRE<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09003&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09003\">pMAK42<\/a><br \/>Expression vector under the control of tet inducible promoter. Puromycin serectable.<\/td>\n<td>&#8211;<\/td>\n<td>Puro &amp; DsRed2<\/td>\n<td>Restriction enzyme<\/td>\n<td>tight TRE<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09004&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09004\">pMT-PURO2G<\/a><br \/>Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells<\/td>\n<td>His-V5<\/td>\n<td>Puro &amp; EGFP<\/td>\n<td>Restriction enzyme<\/td>\n<td>MT<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB09005&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB09005\">pMT-PURO2R<\/a><br \/>Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells<\/td>\n<td>His-V5<\/td>\n<td>Puro&amp; DsRed<\/td>\n<td>Restriction enzyme<\/td>\n<td>MT<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB08531&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08531\">pCoPURO<\/a><br \/>Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.<\/td>\n<td>n.a.<\/td>\n<td>Puro<\/td>\n<td>n.a.<\/td>\n<td>copia<\/td>\n<td>Amp<\/td>\n<\/tr>\n<tr>\n<td>RDB08035&nbsp;<a href=\"https:\/\/brc.riken.jp\/dna\/RDB08035\">pUAST-GFPN-lacZ<\/a><br \/>P-element mediated gene transfer vector of GFP-N and lacZ.<\/td>\n<td>n.a.<\/td>\n<td>GFP-lacZ<\/td>\n<td>n.a.<\/td>\n<td>Gal4-UAS<\/td>\n<td>Amp<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<hr \/>\n<p><a name=\"plant\"><\/a><\/p>\n<h4 class=\"green\">Plant Experiment<\/h4>\n<p>&nbsp;<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB02933<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB02933\">pBAc3<\/a><\/td>\n<td>pBAc3 enhancer trap vector in rice.<\/td>\n<\/tr>\n<tr>\n<td>RDB08246<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB08246\">pCIYVV-Pst\/CP<\/a><\/td>\n<td>Plant expression vector constructed from clover yellow vein virus.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Please visit <a href=\"https:\/\/epd.brc.riken.jp\/en\/\">the Experimental Plant Division<\/a> for more plant DNA clones such as <a href=\"https:\/\/epd.brc.riken.jp\/en\/archives\/4813\">pGWB vectors developed by Prof. Nakagawa Lab<\/a>.<\/p>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.03 (T.M.)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>| Mammalian | shRNA expression | in vitro transcrioption | Supernova series | Gene amplification | Insect cell | Plant | See also &#8220;Viral &#038; Transposon Vector Plasmids (backbone)&#8220;. Mammalian expression Catalog num.&nbsp;Clone nameDescription Epitope tag Antibiotic\/visual marker Promoter Cloning Antibiotics (bacteria) RDB07939&nbsp;pEF-BOSMammalian expression vector with human EF-1a. n.a. n.a. EF-1 alpha Restriction enzyme Amp RDB07940&nbsp;pEF-BOS-EXMammalian expression vector with human EF-1a. n.a. n.a. EF-1 alpha Restriction enzyme Amp RDB18970&nbsp;pNEF-BOS-EXMammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene. n.a. G418 EF-1 alpha Restriction enzyme Amp RDB18971&nbsp;pPEF-BOS-EXMammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene. n.a. puromycin EF-1 alpha Restriction enzyme Amp RDB18972&nbsp;pEF-Flag-EXMammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein. FLAG n.a. EF-1 alpha Restriction enzyme Amp RDB18973&nbsp;pEF321-Flag-ExMammalian expression vector based on human EF-1 alpha promoter. FLAG n.a. EF-1 alpha Restriction enzyme Amp RDB18974&nbsp;pEF-FcExpression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region. n.a. n.a. EF-1 alpha Restriction enzyme Amp &nbsp; Catalog num.&nbsp;Clone nameDescription Epitope tag Antibiotic\/visual marker Promoter Cloning Antibiotics (bacteria) RDB14213&nbsp;pcDNA3.1-myc-GFPExpression vector of N-terminally myc-GFP-tagged proteins in mammalian cells. myc-GFP Neo CMV Restriction enzyme Amp [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8681,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8692","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8692","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8692"}],"version-history":[{"count":3,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8692\/revisions"}],"predecessor-version":[{"id":8697,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8692\/revisions\/8697"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8681"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8692"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}