{"id":8687,"date":"2025-05-03T16:27:50","date_gmt":"2025-05-03T07:27:50","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8687"},"modified":"2025-05-03T16:28:31","modified_gmt":"2025-05-03T07:28:31","slug":"cloningvecen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/cloningen\/cloningvecen","title":{"rendered":"Cloning vectors"},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h3>Empty Backbone Vector<\/h3>\n<hr width=\"50%\" \/>\n<a name=\"cloningvector\"><\/a><\/p>\n<h4>Cloning vector<\/h4>\n<ul>\n<li>Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli.<br \/>\nMiyazaki, K.<br \/>\nBiotechniques 58 (2): 86-88 (2015). PubMed PMID 25652032. <\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<th>Catalog#<\/th>\n<th>Resource name<\/th>\n<th>Description<\/th>\n<\/td>\n<\/tr>\n<tr>\n<td>RDB17947<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17947\">pUC18K_ePAG2<\/a><\/td>\n<td>Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).<\/td>\n<\/tr>\n<tr>\n<td>RDB17948<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17948\">pUC18Z_ePAG2<\/a><\/td>\n<td>Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,1,10)<\/script>RDB18372<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18372\">pUC18K_mPAG2<\/a><\/td>\n<td>Cloning vector harboring PheS counterselection marker. Kanamycin resistance.<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,1,10)<\/script>RDB18373<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18373\">pUC18Z_mPAG2<\/a><\/td>\n<td>Cloning vector harboring PheS counterselection marker. Zeocin resistance.<\/td>\n<\/tr>\n<tr>\n<td><script>newImage(2021,1,10)<\/script>RDB18374<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18374\">pUC18C_mPAG2<\/a><\/td>\n<td>Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.<br \/>\nMotohashi, K.<br \/>\nSci. Rep. 9 (1): 6417 (2019). PubMed PMID 31015513.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB17479<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17479\">pCRT<\/a><\/td>\n<td>Plasmid vector for TA-cloning of PCR-products.<\/td>\n<\/tr>\n<tr>\n<td>RDB17481<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17481\">pCRZero<\/a><\/td>\n<td>Plasmid vector for blunt-end cloning of PCR-products.<\/td>\n<\/tr>\n<tr>\n<td>RDB17480<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17480\">pCRZeroT<\/a><\/td>\n<td>Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17479\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB17\/RDB17479z.png\" alt=\"\" width=\"300\" \/><\/a><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17481\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB17\/RDB17481z.png\" alt=\"\" width=\"300\" \/><\/a><a href=\"https:\/\/brc.riken.jp\/dna\/RDB17480\"><img decoding=\"async\" src=\"https:\/\/dnaconda.riken.jp\/png1\/RDB17\/RDB17480z.png\" alt=\"\" width=\"300\" \/><\/a>\n<\/li>\n<hr width=\"50%\" \/>\n<li>Engineering FRET constructs using CFP and YFP.<br \/>\nShimozono, S., Miyawaki, A.<br \/>\nMethods Cell Biol. 85: 381-393 (2008). PubMed PMID 18155471.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB15126<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15126\">pBS coupler 1<\/a><\/td>\n<td>Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].<\/td>\n<\/tr>\n<tr>\n<td>RDB15127<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15127\">pBS coupler 2<\/a><\/td>\n<td>Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].<\/td>\n<\/tr>\n<tr>\n<td>RDB15128<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15128\">pBS coupler 3<\/a><\/td>\n<td>Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].<\/td>\n<\/tr>\n<tr>\n<td>RDB15129<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15129\">pBS coupler 4<\/a><\/td>\n<td>Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].<\/td>\n<\/tr>\n<tr>\n<td>RDB15130<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15130\">pBS coupler 5<\/a><\/td>\n<td>Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].<\/td>\n<\/tr>\n<tr>\n<td>RDB15131<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15131\">pBS coupler 6<\/a><\/td>\n<td>Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<hr width=\"50%\" \/>\n<li>&nbsp;<br \/>\n<table>\n<tbody>\n<tr>\n<th>Catalog num.<\/th>\n<th>Clone name<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td>RDB03699<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB03699\">pURV19-10<\/a><\/td>\n<td>Cloning vector based on pUC19 with EcoRV site.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.03 (T.M.)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Empty Backbone Vector Cloning vector Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Miyazaki, K. Biotechniques 58 (2): 86-88 (2015). PubMed PMID 25652032. Catalog# Resource name Description RDB17947 pUC18K_ePAG2 Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS). RDB17948 pUC18Z_ePAG2 Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS). RDB18372 pUC18K_mPAG2 Cloning vector harboring PheS counterselection marker. Kanamycin resistance. RDB18373 pUC18Z_mPAG2 Cloning vector harboring PheS counterselection marker. Zeocin resistance. RDB18374 pUC18C_mPAG2 Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance. A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Motohashi, K. Sci. Rep. 9 (1): 6417 (2019). PubMed PMID 31015513. Catalog num. Clone name Description RDB17479 pCRT Plasmid vector for TA-cloning of PCR-products. RDB17481 pCRZero Plasmid vector for blunt-end cloning of PCR-products. RDB17480 pCRZeroT Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products. Engineering FRET constructs using CFP and YFP. Shimozono, S., Miyawaki, A. Methods Cell Biol. 85: 381-393 (2008). PubMed PMID 18155471. Catalog num. Clone name Description RDB15126 pBS coupler 1 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15127 pBS coupler 2 Cloning vector to add [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8681,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8687","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8687","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8687"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8687\/revisions"}],"predecessor-version":[{"id":8691,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8687\/revisions\/8691"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8681"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}