{"id":8653,"date":"2025-05-03T16:17:12","date_gmt":"2025-05-03T07:17:12","guid":{"rendered":"http:\/\/dna.brc.riken.jp\/en\/?page_id=8653"},"modified":"2025-05-03T16:17:49","modified_gmt":"2025-05-03T07:17:49","slug":"sensormiscen","status":"publish","type":"page","link":"http:\/\/dna.brc.riken.jp\/en\/biosensor01en\/sensormiscen","title":{"rendered":"Biosensor misc."},"content":{"rendered":"<p><!-- js calling begin --><code><script type=\"text\/javascript\" src=\"\/en\/wp-content\/uploads\/js\/functions_list.js\"><\/script><\/code><!-- js calling end --><\/p>\n<h2>Biosensor &amp; Indicator<\/h2>\n<h3>Curated topics<\/h3>\n<h4>Bilirubin indicator UnaG, BReleaCa<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin.<br \/>\nShitashima, Y., Shimozawa, T., Asahi, T., Miyawaki, A.<br \/>\nBiochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PubMed PMID 29395087.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15908<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15908\">pRSETB-BReleaCa<\/td>\n<td>Bacteria expression vector of UnaG\/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.<\/td>\n<\/tr>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15909<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15909\">pcDNA3-BReleaCa<\/a><\/td>\n<td>Expression vector of UnaG\/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage.<br \/>\nTakeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S.<br \/>\nSci. Rep. 5: 10488 (2015). PubMed PMID 25990790.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB13504<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB13504\">pET-His UNAG<\/a><\/td>\n<td>Expression clone of eel UnaG in E.coli.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>A bilirubin-inducible fluorescent protein from eel muscle.<br \/>\nKumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A.<br \/>\nCell 153 (7): 1602-1611(2013). PubMed PMID 23768684.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15703<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15703\">UnaG\/pcDNA3-FLAG<\/a><\/td>\n<td>Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.<\/td>\n<\/tr>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15704<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15704\">UnaG\/pGEX-2T<\/a><\/td>\n<td>xpression vector of eel UnaG, bilirubin-inducible fluorescent protein.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<h4>Caspase activity indicator<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>A high-throughput method for development of FRET-based indicators for proteolysis.<br \/>\nNagai, T., Miyawaki, A.<br \/>\nBiochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PubMed PMID 15158444. <\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15705<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15705\">SCAT3.1\/pcDNA4 HMB<\/a><\/td>\n<td>Expression vector of FRET based apoptosis probe.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Cyan-emitting and orange-emitting fluorescent proteins as a donor\/acceptor pair for fluorescence resonance energy transfer.<br \/>\nKarasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A.<br \/>\nBiochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15240<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15240\">MiCy-DEVD-mKO1\/pCS2<\/a><\/td>\n<td>Expression vector of FRET based caspase3 activity monitoring probe.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<h4>Concentration of Protein in cells<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.<br \/>\nMorikawa, J.T., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, M.T.<br \/>\nSci. Rep. 6: 22342, 2016. PubMed PMID: 26956628<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB14203<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14203\">pPAL7_GimRET (CFP-YFP1G\/pPAL7) <\/a><\/td>\n<td>Expression vector of GimRET (Glycine inserted mutant FRET probe) for E. coli.<\/td>\n<\/tr>\n<tr>\n<td>RDB14204<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB14204\">pGimRET (CFP-YFP1G\/pECFP) <\/a><\/td>\n<td>Expression vector of GimRET (Glycine inserted mutant FRET probe) for mammalian cells.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<h4>RA indicator<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Visualization of an endogenous retinoic acid gradient across embryonic development.<br \/>\nShimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A.<br \/>\nNature 496 (7445): 363-366 (2013). PubMed PMID 23563268.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15249<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15249\">GEPRA-B<\/a><\/td>\n<td>Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.<\/td>\n<\/tr>\n<tr>\n<td>RDB15250<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15250\">GEPRA-G<\/a><\/td>\n<td>Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.<\/td>\n<\/tr>\n<tr>\n<td>RDB15251<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15251\">GEPRA-AA<\/a><\/td>\n<td>Genetically encoded indicator for RA, GEPRA. Low-affinity verson of the GEPRA-B.<\/td>\n<\/tr>\n<tr>\n<td>RDB15252<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15252\">GEPRA-B\/ pT2KXIGdeltain<\/a><\/td>\n<td>Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.<\/td>\n<\/tr>\n<tr>\n<td>RDB15253<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15253\">GEPRA-G\/ pT2KXIGdeltain<\/a><\/td>\n<td>Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.<\/td>\n<\/tr>\n<tr>\n<td>RDB15254<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15254\">GEPRA-AA\/ pT2KXIGdeltain<\/a><\/td>\n<td>Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish. Low-affinity verson of the GEPRA-B.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<h4>Rab11: active Rab11 visualization<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11.<br \/>\nOsaki F, Matsui T, Hiragi S, Homma Y, Fukuda M.<br \/>\nJ. Cell Sci. 134 (7): jcs257311 (2021). PubMed PMID 33712449.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB18788<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18788\">pMRX-puro-EGFP-RBD11<\/a><\/td>\n<td>Retroviral expression vector for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) tagged with EGFP.<\/td>\n<\/tr>\n<tr>\n<td>RDB18789<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18789\">pMRX-puro-EGFP-RBD11-mut<\/a><\/td>\n<td>Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.<\/td>\n<\/tr>\n<tr>\n<td>RDB18790<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18790\">pMRX-puro-EGFP-2xRBD11<\/a><\/td>\n<td>Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.<\/td>\n<\/tr>\n<tr>\n<td>RDB18791<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18791\">pMRX-puro-EGFP-2xRBD11-mut<\/a><\/td>\n<td>Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.<\/td>\n<\/tr>\n<tr>\n<td>RDB18792<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18792\">pMRX-puro-EGFP-FM-RBD11<\/a><\/td>\n<td>Retroviral expression vector of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) tagged with EGFP.<\/td>\n<\/tr>\n<tr>\n<td>RDB18793<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB18793\">pMRX-puro-EGFP-FM-RBD11-mut<\/a><\/td>\n<td>Retroviral expression vector of negative control of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) mutant tagged with EGFP.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<h4>Temperature indicator<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide.<br \/>\nVu, C.Q., Fukushima, S.I., Wazawa, T., Nagai, T.<br \/>\nSci. Rep. 11 (1): 16519 (2021). PubMed PMID 34389773.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB19861<script>newImage(2023,5,20)<\/script><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19861\">ELP-TEMP\/pcDNA3.1(-)<\/td>\n<td>Vector of ELP-TEMP, a genetically encoded highly-sensitive temperature indicator, for mammalian expression.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response.<br \/>\nNakano, M., Arai, Y., Kotera, I., Okabe, K., Kamei, Y., Nagai, T.<br \/>\nPLoS One 12 (2): e0172344 (2017). PubMed PMID 28212432.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB19869<script>newImage(2023,5,20)<\/script><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19869\">gTEMP\/pcDNA3<\/td>\n<td>Vector of gTEMP, a genetically-encoded temperature indicator, for mammalian expression.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<h4>Voltage indicator<\/h4>\n<ul style=\"line-height: 1.5em;\">\n<li>Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.<br \/>\nInagaki, S., Tsutsui, H., Suzuki, K., Agetsuma, M., Arai, Y., Jinno, Y., Bai, G., Daniels, M.J., Okamura, Y., Matsuda, T., Nagai, T.<br \/>\nSci. Rep. 7: 42398 (2017). PubMed PMID 28205521.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB19881<script>newImage(2023,5,20)<\/script><\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB19881\">LOTUS-V\/pcDNA3<\/td>\n<td>Vector of LOTUS-V, a fluorescent protein, for mammalian expression.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<li>Improving membrane voltage measurements using FRET with new fluorescent proteins.<br \/>\nTsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A.<br \/>\nNat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15262<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15262\">Mermaid<\/td>\n<td>Voltage indicator Mermaid.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<li>Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase.<br \/>\nTsutsui, H., Jinno, Y., Tomita, A., Niino, Y., Yamada, Y., Mikoshiba, K., Miyawaki, A., Okamura, Y.<br \/>\nJ. Physiol. 591 (18): 4427-4437 (2013). PubMed PMID 23836686.<\/p>\n<table>\n<tbody>\n<tr>\n<th>Catalog no.<\/td>\n<th>Name of resource<\/td>\n<th>Descriptiion<\/td>\n<\/tr>\n<tr>\n<td>RDB15263<\/td>\n<td><a href=\"https:\/\/brc.riken.jp\/dna\/RDB15263\">Mermaid2<\/td>\n<td>Voltage probe Mermaid2.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<hr \/>\n<p style=\"text-align:left;\">2025.05.03 (T.M.)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biosensor &amp; Indicator Curated topics Bilirubin indicator UnaG, BReleaCa A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Shitashima, Y., Shimozawa, T., Asahi, T., Miyawaki, A. Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PubMed PMID 29395087. Catalog no. Name of resource Descriptiion RDB15908 pRSETB-BReleaCa Bacteria expression vector of UnaG\/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium. Catalog no. Name of resource Descriptiion RDB15909 pcDNA3-BReleaCa Expression vector of UnaG\/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium. Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Takeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S. Sci. Rep. 5: 10488 (2015). PubMed PMID 25990790. Catalog no. Name of resource Descriptiion RDB13504 pET-His UNAG Expression clone of eel UnaG in E.coli. A bilirubin-inducible fluorescent protein from eel muscle. Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A. Cell 153 (7): 1602-1611(2013). PubMed PMID 23768684. Catalog no. Name of resource Descriptiion RDB15703 UnaG\/pcDNA3-FLAG Expression vector of eel UnaG, bilirubin-inducible fluorescent protein. Catalog no. Name of resource Descriptiion RDB15704 UnaG\/pGEX-2T xpression vector of eel UnaG, bilirubin-inducible fluorescent protein. Caspase activity indicator [&hellip;]<\/p>\n","protected":false},"author":13,"featured_media":0,"parent":8590,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8653","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8653","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8653"}],"version-history":[{"count":2,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8653\/revisions"}],"predecessor-version":[{"id":8657,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8653\/revisions\/8657"}],"up":[{"embeddable":true,"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8590"}],"wp:attachment":[{"href":"http:\/\/dna.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8653"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}